MD15G1052700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0016386
Physical Location & Seq
Forward (+)
3610628 .. 3611648
1021 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1052700.v1.1.491

Sequence Viewer

Length: 681 bp
ATGGGAAGCGGCTTCTGCACTTTGAAGAGGAATATTATGGCCGGTGTTGCAACGACACTGGTTTGCGTCAAGCAAGTGAAGCAAGAGGAGTCGGAAGAGTGGGACGAGAGCATGCCATTGCCCGGAGACATCATTGAAGGGGTTTATAATTCCGAGAGCAGTGACAATATTGGTGACGAGTTTTTCGTGCCTACGAAAGCCAAGGCTGAGCTTATGTCGCAGCTTGCTAGGCTCAGCAATGTAGAGGTCATTTGGGTGAAGGTGAGGAGGGGTGATAGCACTCTCAAGCTCCGATCGCGCGTGGCTGAGAGGCCTTCGATGCTTCAGAAAAAGTATACGATCAAAGCGGCTACAGATGATAGACATGTTGCAGTTTTGGAGGACTTGACCTCGGATCAATGCACTGAGCTGCAAGAAATGAGCAGGAGTTTGGTGAACGTGGAATACAGGGGACACAACAAAGGGGGAGTTAAGTATGACTGGAAGATGAAAGTGGACAGCTACTTGCCCGATAAACGGTGCACGATCGTTAGCTCCATTCTGTTCATGCCGCTGCCGGGTGAGTACTGCACCGAGGCCACAACAGCCCGTTGCATGGCTTGGTTTATTGCAGCAGTTGCTTCCGGAGCTCCGCTAATTTTTGTTAACATCCAAACAGAACAGATCCTGTCGTCGGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

227

Amino Acids

25.26

Weight (kDa)

5.58

Isoelectric Point (pI)

72.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016386)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g08721
malus_domestica MD08G1069400.v1.1 MD15G1052700.v1.1
prunus_persica Prupe.1G406600_v2.0.a1
pyrus_communis pycom08g05720
rosa_chinensis RchiOBHm_Chr1g0343961
rosa_laevigata RLG00000028943
rosa_multiflora Rmu_ssc0000434.1_g000040
rosa_roxburghii Rroxscaffold_4G00310210
rosa_rugosa Rorug01G0170000
rosa_samantha Rh1AG186600 Rh1BG153000 Rh1CG172000 Rh1DG185300
rosa_wichuraiana Rw1G015390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 147
AccI GTMKAC 1 cut(s) 335
AccII CGCG 2 cut(s) 298, 300
AccIII TCCGGA 1 cut(s) 623
AciI CCGC 4 cut(s) 9, 347, 551, 632
AclWI GGATC 2 cut(s) 402, 658
AcoI YGGCCR 1 cut(s) 39
AcuI CTGAAG 1 cut(s) 308
AfaI GTAC 1 cut(s) 566
AfiI CCNNNNNNNGG 5 cut(s) 122, 516, 557, 595, 673
AflIII ACRYGT 1 cut(s) 364
AgsI TTSAA 2 cut(s) 25, 137
AluBI AGCT 7 cut(s) 211, 223, 289, 409, 501, 534, 629
AluI AGCT 7 cut(s) 211, 223, 289, 409, 501, 534, 629
Alw21I GWGCWC 2 cut(s) 524, 631
Alw26I GTCTC 1 cut(s) 120
Alw44I GTGCAC 1 cut(s) 520
AlwI GGATC 2 cut(s) 402, 658
AlwNI CAGNNNCTG 1 cut(s) 667
Aor13HI TCCGGA 1 cut(s) 623
AoxI GGCC 3 cut(s) 39, 311, 576
ApaLI GTGCAC 1 cut(s) 520
ApeKI GCWGC 4 cut(s) 220, 409, 553, 611
ArsI GACNNNNNNTTYG 2 cut(s) 167, 199
AspLEI GCGC 1 cut(s) 300
AsuC2I CCSGG 2 cut(s) 123, 558
AsuHPI GGTGA 6 cut(s) 185, 268, 274, 284, 445, 572
BaeGI GKGCMC 1 cut(s) 524
BanII GRGCYC 1 cut(s) 631
Bbv12I GWGCWC 2 cut(s) 524, 631
BbvI GCAGC 4 cut(s) 232, 396, 540, 623
BcnI CCSGG 2 cut(s) 123, 558
BcoDI GTCTC 1 cut(s) 120
BfaI CTAG 1 cut(s) 228
BfmI CTRYAG 1 cut(s) 351
BisI GCNGC 7 cut(s) 10, 221, 348, 410, 551, 554, 612
BlpI GCTNAGC 2 cut(s) 207, 233
BlsI GCNGC 7 cut(s) 11, 222, 349, 411, 552, 555, 613
BmcAI AGTACT 1 cut(s) 566
Bme1390I CCNGG 2 cut(s) 123, 558
BmrFI CCNGG 2 cut(s) 123, 558
BmsI GCATC 1 cut(s) 309
Bpu1102I GCTNAGC 2 cut(s) 207, 233
BpuEI CTTGAG 1 cut(s) 269
BpuMI CCSGG 2 cut(s) 123, 558
BsaJI CCNNGG 3 cut(s) 201, 390, 573
BsaWI WCCGGW 1 cut(s) 623
Bsc4I CCNNNNNNNGG 5 cut(s) 122, 516, 557, 595, 673
Bse118I RCCGGY 1 cut(s) 41
Bse1I ACTGG 2 cut(s) 63, 485
Bse3DI GCAATG 2 cut(s) 116, 244
BseAI TCCGGA 1 cut(s) 623
BseDI CCNNGG 3 cut(s) 201, 390, 573
BseGI GGATG 1 cut(s) 648
BseLI CCNNNNNNNGG 5 cut(s) 122, 516, 557, 595, 673
BseMI GCAATG 2 cut(s) 116, 244
BseMII CTCAG 4 cut(s) 198, 247, 297, 396
BseNI ACTGG 2 cut(s) 63, 485
BseRI GAGGAG 2 cut(s) 101, 280
BseSI GKGCMC 1 cut(s) 524
BseXI GCAGC 4 cut(s) 232, 396, 540, 623
BsgI GTGCAG 1 cut(s) 553
Bsh1236I CGCG 2 cut(s) 298, 300
Bsh1285I CGRYCG 2 cut(s) 296, 528
BshFI GGCC 3 cut(s) 41, 313, 578
BsiEI CGRYCG 2 cut(s) 296, 528
BsiHKAI GWGCWC 2 cut(s) 524, 631
BsiSI CCGG 4 cut(s) 42, 123, 557, 624
BslFI GGGAC 2 cut(s) 116, 465
BslI CCNNNNNNNGG 5 cut(s) 122, 516, 557, 595, 673
BsmAI GTCTC 1 cut(s) 120
BsmFI GGGAC 2 cut(s) 116, 465
BsnI GGCC 3 cut(s) 41, 313, 578
Bsp1286I GDGCHC 2 cut(s) 524, 631
Bsp13I TCCGGA 1 cut(s) 623
Bsp143I GATC 5 cut(s) 293, 339, 394, 525, 663
Bsp1720I GCTNAGC 2 cut(s) 207, 233
BspACI CCGC 4 cut(s) 9, 347, 551, 632
BspANI GGCC 3 cut(s) 41, 313, 578
BspCNI CTCAG 4 cut(s) 199, 246, 298, 397
BspEI TCCGGA 1 cut(s) 623
BspFNI CGCG 2 cut(s) 298, 300
BspPI GGATC 2 cut(s) 402, 658
BsrDI GCAATG 2 cut(s) 116, 244
BsrFI RCCGGY 1 cut(s) 41
BsrI ACTGG 2 cut(s) 63, 485
BssAI RCCGGY 1 cut(s) 41
BssECI CCNNGG 3 cut(s) 201, 390, 573
BssMI GATC 5 cut(s) 293, 339, 394, 525, 663
BssNAI GTATAC 1 cut(s) 336
BssT1I CCWWGG 1 cut(s) 201
Bst1107I GTATAC 1 cut(s) 336
Bst4CI ACNGT 1 cut(s) 519
Bst6I CTCTTC 2 cut(s) 20, 90
BstAPI GCANNNNNTGC 1 cut(s) 617
BstC8I GCNNGC 2 cut(s) 113, 225
BstDEI CTNAG 4 cut(s) 207, 233, 306, 405
BstF5I GGATG 1 cut(s) 648
BstFNI CGCG 2 cut(s) 298, 300
BstHHI GCGC 1 cut(s) 300
BstKTI GATC 5 cut(s) 296, 342, 397, 528, 666
BstMAI GTCTC 1 cut(s) 120
BstMBI GATC 5 cut(s) 293, 339, 394, 525, 663
BstMCI CGRYCG 2 cut(s) 296, 528
BstNSI RCATGY 2 cut(s) 115, 368
BstSCI CCNGG 2 cut(s) 121, 556
BstSFI CTRYAG 1 cut(s) 351
BstSLI GKGCMC 1 cut(s) 524
BstUI CGCG 2 cut(s) 298, 300
BstV1I GCAGC 4 cut(s) 232, 396, 540, 623
BstX2I RGATCY 1 cut(s) 663
BstYI RGATCY 1 cut(s) 663
BstZ17I GTATAC 1 cut(s) 336
BsuRI GGCC 3 cut(s) 41, 313, 578
BtsCI GGATG 1 cut(s) 648
BtsI GCAGTG 1 cut(s) 166
BtsIMutI CAGTG 3 cut(s) 56, 166, 402
Cac8I GCNNGC 2 cut(s) 113, 225
CaiI CAGNNNCTG 1 cut(s) 667
CfoI GCGC 1 cut(s) 300
Cfr10I RCCGGY 1 cut(s) 41
CseI GACGC 1 cut(s) 55
Csp6I GTAC 1 cut(s) 565
CviAII CATG 4 cut(s) 112, 365, 547, 595
CviQI GTAC 1 cut(s) 565
DdeI CTNAG 4 cut(s) 207, 233, 306, 405
DpnI GATC 5 cut(s) 295, 341, 396, 527, 665
DpnII GATC 5 cut(s) 293, 339, 394, 525, 663
EaeI YGGCCR 1 cut(s) 39
Eam1104I CTCTTC 2 cut(s) 20, 90
EarI CTCTTC 2 cut(s) 20, 90
Ecl136II GAGCTC 1 cut(s) 629
Eco130I CCWWGG 1 cut(s) 201
Eco147I AGGCCT 1 cut(s) 313
Eco24I GRGCYC 1 cut(s) 631
Eco53kI GAGCTC 1 cut(s) 629
Eco57I CTGAAG 1 cut(s) 308
EcoICRI GAGCTC 1 cut(s) 629
EcoT14I CCWWGG 1 cut(s) 201
EcoT38I GRGCYC 1 cut(s) 631
ErhI CCWWGG 1 cut(s) 201
FaeI CATG 4 cut(s) 115, 368, 550, 598
FaqI GGGAC 2 cut(s) 116, 465
FatI CATG 4 cut(s) 111, 364, 546, 594
FblI GTMKAC 1 cut(s) 335
Fnu4HI GCNGC 7 cut(s) 10, 221, 348, 410, 551, 554, 612
FokI GGATG 1 cut(s) 635
FriOI GRGCYC 1 cut(s) 631
Fsp4HI GCNGC 7 cut(s) 10, 221, 348, 410, 551, 554, 612
FspBI CTAG 1 cut(s) 228
GlaI GCGC 1 cut(s) 299
GluI GCNGC 7 cut(s) 10, 221, 348, 410, 551, 554, 612
HaeIII GGCC 3 cut(s) 41, 313, 578
HapII CCGG 4 cut(s) 42, 123, 557, 624
HgaI GACGC 1 cut(s) 55
HhaI GCGC 1 cut(s) 300
Hin1II CATG 4 cut(s) 115, 368, 550, 598
Hin6I GCGC 1 cut(s) 298
HinP1I GCGC 1 cut(s) 298
HincII GTYRAC 1 cut(s) 646
HindII GTYRAC 1 cut(s) 646
HinfI GANTC 1 cut(s) 89
HpaI GTTAAC 1 cut(s) 646
HpaII CCGG 4 cut(s) 42, 123, 557, 624
HphI GGTGA 6 cut(s) 185, 268, 274, 284, 445, 572
Hpy166II GTNNAC 5 cut(s) 336, 436, 496, 522, 646
Hpy188I TCNGA 5 cut(s) 94, 154, 293, 327, 394
Hpy188III TCNNGA 1 cut(s) 624
Hpy8I GTNNAC 5 cut(s) 336, 436, 496, 522, 646
Hpy99I CGWCG 1 cut(s) 676
HpyAV CCTTC 3 cut(s) 131, 253, 324
HpyCH4III ACNGT 1 cut(s) 519
HpyCH4IV ACGT 1 cut(s) 438
HpyCH4V TGCA 9 cut(s) 18, 50, 371, 402, 412, 522, 570, 594, 611
HpyF3I CTNAG 4 cut(s) 207, 233, 306, 405
HpySE526I ACGT 1 cut(s) 438
Hsp92II CATG 4 cut(s) 115, 368, 550, 598
HspAI GCGC 1 cut(s) 298
Kpn2I TCCGGA 1 cut(s) 623
KspAI GTTAAC 1 cut(s) 646
Kzo9I GATC 5 cut(s) 293, 339, 394, 525, 663
LmnI GCTCC 4 cut(s) 294, 539, 626, 634
LpnPI CCDG 8 cut(s) 44, 55, 136, 409, 433, 466, 570, 637
Lsp1109I GCAGC 4 cut(s) 232, 396, 540, 623
LweI GCATC 1 cut(s) 309
MaeI CTAG 1 cut(s) 228
MaeII ACGT 1 cut(s) 438
MaeIII GTNAC 2 cut(s) 161, 173
MalI GATC 5 cut(s) 295, 341, 396, 527, 665
MboI GATC 5 cut(s) 293, 339, 394, 525, 663
MboII GAAGA 3 cut(s) 37, 107, 496
MflI RGATCY 1 cut(s) 663
MhlI GDGCHC 2 cut(s) 524, 631
MluCI AATT 2 cut(s) 148, 636
MlyI GAGTC 1 cut(s) 98
MmeI TCCRAC 1 cut(s) 72
MnlI CCTC 9 cut(s) 21, 79, 238, 258, 261, 303, 373, 400, 568
MroI TCCGGA 1 cut(s) 623
MseI TTAA 2 cut(s) 471, 645
MslI CAYNNNNRTG 1 cut(s) 254
MspA1I CMGCKG 1 cut(s) 553
MspI CCGG 4 cut(s) 42, 123, 557, 624
MspR9I CCNGG 2 cut(s) 123, 558
MvnI CGCG 2 cut(s) 298, 300
NciI CCSGG 2 cut(s) 123, 558
NdeII GATC 5 cut(s) 293, 339, 394, 525, 663
NlaIII CATG 4 cut(s) 115, 368, 550, 598
NmuCI GTSAC 2 cut(s) 161, 173
NspI RCATGY 2 cut(s) 115, 368
PaeI GCATGC 1 cut(s) 115
PceI AGGCCT 1 cut(s) 313
PciI ACATGT 1 cut(s) 364
PcsI WCGNNNNNNNCGW 1 cut(s) 183
PkrI GCNGC 7 cut(s) 11, 222, 349, 411, 552, 555, 613
Ple19I CGATCG 2 cut(s) 296, 528
PleI GAGTC 1 cut(s) 97
PpsI GAGTC 1 cut(s) 97
PscI ACATGT 1 cut(s) 364
PsiI TTATAA 1 cut(s) 147
Psp124BI GAGCTC 1 cut(s) 631
PsrI GAACNNNNNNTAC 2 cut(s) 428, 460
PstNI CAGNNNCTG 1 cut(s) 667
PsuI RGATCY 1 cut(s) 663
PvuI CGATCG 2 cut(s) 296, 528
RsaI GTAC 1 cut(s) 566
RsaNI GTAC 1 cut(s) 565
RseI CAYNNNNRTG 1 cut(s) 254
SacI GAGCTC 1 cut(s) 631
SaqAI TTAA 2 cut(s) 471, 645
SatI GCNGC 7 cut(s) 10, 221, 348, 410, 551, 554, 612
Sau3AI GATC 5 cut(s) 293, 339, 394, 525, 663
ScaI AGTACT 1 cut(s) 566
SchI GAGTC 1 cut(s) 98
ScrFI CCNGG 2 cut(s) 123, 558
SduI GDGCHC 2 cut(s) 524, 631
SfaNI GCATC 1 cut(s) 309
SfcI CTRYAG 1 cut(s) 351
SmiMI CAYNNNNRTG 1 cut(s) 254
SmlI CTYRAG 1 cut(s) 284
SmoI CTYRAG 1 cut(s) 284
SphI GCATGC 1 cut(s) 115
Sse9I AATT 2 cut(s) 148, 636
SseBI AGGCCT 1 cut(s) 313
SsiI CCGC 4 cut(s) 9, 347, 551, 632
SspI AATATT 2 cut(s) 34, 169
SspMI CTAG 1 cut(s) 228
SstI GAGCTC 1 cut(s) 631
StuI AGGCCT 1 cut(s) 313
StyD4I CCNGG 2 cut(s) 121, 556
StyI CCWWGG 1 cut(s) 201
TaaI ACNGT 1 cut(s) 519
TaiI ACGT 1 cut(s) 441
TaqI TCGA 1 cut(s) 317
TasI AATT 2 cut(s) 148, 636
TatI WGTACW 1 cut(s) 564
TauI GCSGC 3 cut(s) 12, 350, 553
Tru1I TTAA 2 cut(s) 471, 645
Tru9I TTAA 2 cut(s) 471, 645
TscAI CASTG 3 cut(s) 63, 166, 409
TseFI GTSAC 2 cut(s) 161, 173
TseI GCWGC 4 cut(s) 220, 409, 553, 611
Tsp45I GTSAC 2 cut(s) 161, 173
TspDTI ATGAA 2 cut(s) 503, 535
TspRI CASTG 3 cut(s) 63, 166, 409
VneI GTGCAC 1 cut(s) 520
XceI RCATGY 2 cut(s) 115, 368
XmiI GTMKAC 1 cut(s) 335
XspI CTAG 1 cut(s) 228
ZrmI AGTACT 1 cut(s) 566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.