Rmu_ssc0000434.1_g000040

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000434.1
OG0016386
Physical Location & Seq
Reverse (-)
172940 .. 174395
1456 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000434.1_g000040.1.cds

Sequence Viewer

Length: 954 bp
atgtgcactgttcagacgcttgtgtgtgtgaaacaagtgaagcaagagattgcagaagagtggaatgagactatgccattgcctggagacatcatcgaaggcttttacaatggcgagagtgatgagttttttgtgcccaccaaagctaagtctgagcttagttcacagcttgctaagataagcagccaacatatagaggtcatatgggtgaaggtcagaaggggtgataccactctcaagctccgggcgcgtgttgcggtggagaggctttccatgcttcaaagaaagtatagcatcaaagcagccacagatgacagacatgttgcggtcataggggacttgacctcggatcaatgcaccgagctgcaagaaatgagcaggagtttggtaaatgtggaaaacagaggatataacaaaaggggagtaaaatatgactggaagatgaaagtgagcaagtatttacctgaccaacgttgcacggttgttagctctattctgttcatgccaatgcagggtgagtactgtactgaggccacaacagccaggtgcatggcttggttttctgcagcagtttcttcaggggctcctcttgtttttgttaatattcaaaccgaacagactgtgacctcggggttgatatgcatctactctgttgcaaaggaaacaccagcagaccgagcagggcttaggagtctccatgaggaagcatatgcaagtggttacctccttgtcatgtccagattagagggcaagagcctaatgccttcgcatgctgacatttcagggctcattcactgttgcgatcacaccgaaattaagaacactctattctcggcgatagaccagatggacagaattcaaattcatgttatggcgtggtctaaccaagcacatcctactactcccaataccacagcagttggtgccacaatgctacgcccaagaccatcgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

317

Amino Acids

35.43

Weight (kDa)

7.07

Isoelectric Point (pI)

54.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016386)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g08721
malus_domestica MD08G1069400.v1.1 MD15G1052700.v1.1
prunus_persica Prupe.1G406600_v2.0.a1
pyrus_communis pycom08g05720
rosa_chinensis RchiOBHm_Chr1g0343961
rosa_laevigata RLG00000028943
rosa_multiflora Rmu_ssc0000434.1_g000040
rosa_roxburghii Rroxscaffold_4G00310210
rosa_rugosa Rorug01G0170000
rosa_samantha Rh1AG186600 Rh1BG153000 Rh1CG172000 Rh1DG185300
rosa_wichuraiana Rw1G015390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 923
AccB7I CCANNNNNTGG 1 cut(s) 83
AccII CGCG 1 cut(s) 250
AciI CCGC 2 cut(s) 257, 326
AclI AACGTT 1 cut(s) 472
AclWI GGATC 1 cut(s) 357
AcsI RAATTY 2 cut(s) 855, 861
AcuI CTGAAG 1 cut(s) 561
AfaI GTAC 2 cut(s) 521, 526
AfiI CCNNNNNNNGG 2 cut(s) 83, 512
AflIII ACRYGT 1 cut(s) 319
AgsI TTSAA 3 cut(s) 281, 608, 860
AjnI CCWGG 2 cut(s) 82, 542
AluBI AGCT 6 cut(s) 146, 157, 169, 241, 364, 489
AluI AGCT 6 cut(s) 146, 157, 169, 241, 364, 489
Alw21I GWGCWC 1 cut(s) 8
Alw26I GTCTC 3 cut(s) 62, 81, 698
Alw44I GTGCAC 1 cut(s) 4
AlwI GGATC 1 cut(s) 357
Ama87I CYCGRG 1 cut(s) 628
AoxI GGCC 1 cut(s) 531
ApaLI GTGCAC 1 cut(s) 4
ApeKI GCWGC 4 cut(s) 183, 302, 364, 566
ApoI RAATTY 2 cut(s) 855, 861
AspLEI GCGC 1 cut(s) 250
AsuC2I CCSGG 1 cut(s) 245
AsuHPI GGTGA 3 cut(s) 220, 236, 527
AvaI CYCGRG 1 cut(s) 628
BaeGI GKGCMC 2 cut(s) 8, 138
BanI GGYRCC 1 cut(s) 923
BanII GRGCYC 2 cut(s) 586, 789
BarI GAAGNNNNNNTAC 2 cut(s) 211, 243
Bbv12I GWGCWC 1 cut(s) 8
BbvI GCAGC 4 cut(s) 195, 314, 351, 578
BccI CCATC 1 cut(s) 841
BciT130I CCWGG 2 cut(s) 84, 544
BcnI CCSGG 1 cut(s) 245
BcoDI GTCTC 3 cut(s) 62, 81, 698
BfmI CTRYAG 1 cut(s) 564
BisI GCNGC 4 cut(s) 184, 303, 365, 567
BlsI GCNGC 4 cut(s) 185, 304, 366, 568
BmcAI AGTACT 1 cut(s) 521
Bme1390I CCNGG 3 cut(s) 84, 245, 544
BmeT110I CYCGRG 1 cut(s) 628
BmiI GGNNCC 2 cut(s) 585, 925
BmrFI CCNGG 3 cut(s) 84, 245, 544
BmsI GCATC 2 cut(s) 303, 651
BpmI CTGGAG 1 cut(s) 105
Bpu10I CCTNAGC 1 cut(s) 686
BpuEI CTTGAG 1 cut(s) 221
BpuMI CCSGG 1 cut(s) 245
BsaBI GATNNNNATC 1 cut(s) 641
BsaJI CCNNGG 2 cut(s) 345, 627
Bsc4I CCNNNNNNNGG 2 cut(s) 83, 512
Bse1I ACTGG 1 cut(s) 440
Bse3DI GCAATG 1 cut(s) 77
Bse8I GATNNNNATC 1 cut(s) 641
BseBI CCWGG 2 cut(s) 84, 544
BseDI CCNNGG 2 cut(s) 345, 627
BseGI GGATG 1 cut(s) 892
BseJI GATNNNNATC 1 cut(s) 641
BseLI CCNNNNNNNGG 2 cut(s) 83, 512
BseMI GCAATG 1 cut(s) 77
BseMII CTCAG 2 cut(s) 144, 519
BseNI ACTGG 1 cut(s) 440
BseRI GAGGAG 1 cut(s) 576
BseSI GKGCMC 2 cut(s) 8, 138
BseXI GCAGC 4 cut(s) 195, 314, 351, 578
Bsh1236I CGCG 1 cut(s) 250
BshFI GGCC 1 cut(s) 533
BshNI GGYRCC 1 cut(s) 923
BsiHKAI GWGCWC 1 cut(s) 8
BsiHKCI CYCGRG 1 cut(s) 628
BsiSI CCGG 1 cut(s) 244
BslFI GGGAC 1 cut(s) 350
BslI CCNNNNNNNGG 2 cut(s) 83, 512
BsmAI GTCTC 3 cut(s) 62, 81, 698
BsmFI GGGAC 1 cut(s) 350
BsnI GGCC 1 cut(s) 533
BsoBI CYCGRG 1 cut(s) 628
Bsp1286I GDGCHC 4 cut(s) 8, 138, 586, 789
Bsp143I GATC 2 cut(s) 349, 802
BspACI CCGC 2 cut(s) 257, 326
BspANI GGCC 1 cut(s) 533
BspCNI CTCAG 2 cut(s) 145, 520
BspFNI CGCG 1 cut(s) 250
BspLI GGNNCC 2 cut(s) 585, 925
BspMAI CTGCAG 1 cut(s) 568
BspPI GGATC 1 cut(s) 357
BspT107I GGYRCC 1 cut(s) 923
BsrDI GCAATG 1 cut(s) 77
BsrI ACTGG 1 cut(s) 440
BssECI CCNNGG 2 cut(s) 345, 627
BssMI GATC 2 cut(s) 349, 802
Bst2UI CCWGG 2 cut(s) 84, 544
Bst4CI ACNGT 5 cut(s) 10, 481, 524, 622, 797
Bst6I CTCTTC 1 cut(s) 51
BstAPI GCANNNNNTGC 1 cut(s) 923
BstC8I GCNNGC 2 cut(s) 171, 771
BstDEI CTNAG 6 cut(s) 147, 153, 158, 174, 528, 686
BstEII GGTNACC 1 cut(s) 719
BstF5I GGATG 1 cut(s) 892
BstFNI CGCG 1 cut(s) 250
BstHHI GCGC 1 cut(s) 250
BstKTI GATC 2 cut(s) 352, 805
BstMAI GTCTC 3 cut(s) 62, 81, 698
BstMBI GATC 2 cut(s) 349, 802
BstMWI GCNNNNNNNGC 6 cut(s) 247, 254, 274, 539, 677, 923
BstNI CCWGG 2 cut(s) 84, 544
BstNSI RCATGY 2 cut(s) 323, 773
BstPI GGTNACC 1 cut(s) 719
BstSCI CCNGG 3 cut(s) 82, 243, 542
BstSFI CTRYAG 1 cut(s) 564
BstSLI GKGCMC 2 cut(s) 8, 138
BstUI CGCG 1 cut(s) 250
BstV1I GCAGC 4 cut(s) 195, 314, 351, 578
BstXI CCANNNNNNTGG 1 cut(s) 550
BsuRI GGCC 1 cut(s) 533
BtsCI GGATG 1 cut(s) 892
BtsIMutI CAGTG 2 cut(s) 6, 793
Cac8I GCNNGC 2 cut(s) 171, 771
CfoI GCGC 1 cut(s) 250
CseI GACGC 1 cut(s) 25
Csp6I GTAC 2 cut(s) 520, 525
CspCI CAANNNNNGTGG 2 cut(s) 901, 936
CviAII CATG 8 cut(s) 274, 320, 502, 550, 698, 733, 770, 866
CviQI GTAC 2 cut(s) 520, 525
DdeI CTNAG 6 cut(s) 147, 153, 158, 174, 528, 686
DpnI GATC 2 cut(s) 351, 804
DpnII GATC 2 cut(s) 349, 802
Eam1104I CTCTTC 1 cut(s) 51
EarI CTCTTC 1 cut(s) 51
Eco24I GRGCYC 2 cut(s) 586, 789
Eco57I CTGAAG 1 cut(s) 561
Eco88I CYCGRG 1 cut(s) 628
Eco91I GGTNACC 1 cut(s) 719
EcoO65I GGTNACC 1 cut(s) 719
EcoRI GAATTC 1 cut(s) 855
EcoRII CCWGG 2 cut(s) 82, 542
EcoT22I ATGCAT 1 cut(s) 644
EcoT38I GRGCYC 2 cut(s) 586, 789
FaeI CATG 8 cut(s) 277, 323, 505, 553, 701, 736, 773, 869
FaqI GGGAC 1 cut(s) 350
FatI CATG 8 cut(s) 273, 319, 501, 549, 697, 732, 769, 865
FauNDI CATATG 2 cut(s) 203, 709
Fnu4HI GCNGC 4 cut(s) 184, 303, 365, 567
FokI GGATG 1 cut(s) 879
FriOI GRGCYC 2 cut(s) 586, 789
Fsp4HI GCNGC 4 cut(s) 184, 303, 365, 567
GlaI GCGC 1 cut(s) 249
GluI GCNGC 4 cut(s) 184, 303, 365, 567
GsuI CTGGAG 1 cut(s) 105
HaeIII GGCC 1 cut(s) 533
HapII CCGG 1 cut(s) 244
HgaI GACGC 1 cut(s) 25
HhaI GCGC 1 cut(s) 250
Hin1II CATG 8 cut(s) 277, 323, 505, 553, 701, 736, 773, 869
Hin6I GCGC 1 cut(s) 248
HinP1I GCGC 1 cut(s) 248
HinfI GANTC 1 cut(s) 691
HpaII CCGG 1 cut(s) 244
HphI GGTGA 3 cut(s) 220, 236, 527
Hpy166II GTNNAC 2 cut(s) 6, 164
Hpy188I TCNGA 4 cut(s) 15, 154, 218, 349
Hpy188III TCNNGA 2 cut(s) 738, 951
Hpy8I GTNNAC 2 cut(s) 6, 164
HpyAV CCTTC 4 cut(s) 92, 205, 213, 774
HpyCH4III ACNGT 5 cut(s) 10, 481, 524, 622, 797
HpyCH4IV ACGT 1 cut(s) 472
HpyF10VI GCNNNNNNNGC 6 cut(s) 247, 254, 274, 539, 677, 923
HpyF3I CTNAG 6 cut(s) 147, 153, 158, 174, 528, 686
HpySE526I ACGT 1 cut(s) 472
Hsp92II CATG 8 cut(s) 277, 323, 505, 553, 701, 736, 773, 869
HspAI GCGC 1 cut(s) 248
Kzo9I GATC 2 cut(s) 349, 802
LmnI GCTCC 2 cut(s) 246, 589
Lsp1109I GCAGC 4 cut(s) 195, 314, 351, 578
LweI GCATC 2 cut(s) 303, 651
MaeII ACGT 1 cut(s) 472
MaeIII GTNAC 2 cut(s) 622, 719
MalI GATC 2 cut(s) 351, 804
MboI GATC 2 cut(s) 349, 802
MboII GAAGA 3 cut(s) 68, 451, 567
MhlI GDGCHC 4 cut(s) 8, 138, 586, 789
MluCI AATT 3 cut(s) 813, 855, 861
MlyI GAGTC 1 cut(s) 700
Mph1103I ATGCAT 1 cut(s) 644
MseI TTAA 2 cut(s) 600, 816
MslI CAYNNNNRTG 2 cut(s) 206, 506
MspI CCGG 1 cut(s) 244
MspR9I CCNGG 3 cut(s) 84, 245, 544
MvaI CCWGG 2 cut(s) 84, 544
MvnI CGCG 1 cut(s) 250
MwoI GCNNNNNNNGC 6 cut(s) 247, 254, 274, 539, 677, 923
NciI CCSGG 1 cut(s) 245
NdeI CATATG 2 cut(s) 203, 709
NdeII GATC 2 cut(s) 349, 802
NlaIII CATG 8 cut(s) 277, 323, 505, 553, 701, 736, 773, 869
NlaIV GGNNCC 2 cut(s) 585, 925
NmeAIII GCCGAG 1 cut(s) 812
NmuCI GTSAC 1 cut(s) 622
NsiI ATGCAT 1 cut(s) 644
NspI RCATGY 2 cut(s) 323, 773
PaeI GCATGC 1 cut(s) 773
PciI ACATGT 1 cut(s) 319
PflMI CCANNNNNTGG 1 cut(s) 83
PkrI GCNGC 4 cut(s) 185, 304, 366, 568
PleI GAGTC 1 cut(s) 699
PpsI GAGTC 1 cut(s) 699
PscI ACATGT 1 cut(s) 319
Psp1406I AACGTT 1 cut(s) 472
Psp6I CCWGG 2 cut(s) 82, 542
PspEI GGTNACC 1 cut(s) 719
PspGI CCWGG 2 cut(s) 82, 542
PspN4I GGNNCC 2 cut(s) 585, 925
PstI CTGCAG 1 cut(s) 568
RsaI GTAC 2 cut(s) 521, 526
RsaNI GTAC 2 cut(s) 520, 525
RseI CAYNNNNRTG 2 cut(s) 206, 506
SaqAI TTAA 2 cut(s) 600, 816
SatI GCNGC 4 cut(s) 184, 303, 365, 567
Sau3AI GATC 2 cut(s) 349, 802
ScaI AGTACT 1 cut(s) 521
SchI GAGTC 1 cut(s) 700
ScrFI CCNGG 3 cut(s) 84, 245, 544
SduI GDGCHC 4 cut(s) 8, 138, 586, 789
SfaNI GCATC 2 cut(s) 303, 651
SfcI CTRYAG 1 cut(s) 564
SmiMI CAYNNNNRTG 2 cut(s) 206, 506
SmlI CTYRAG 1 cut(s) 236
SmoI CTYRAG 1 cut(s) 236
SphI GCATGC 1 cut(s) 773
Sse9I AATT 3 cut(s) 813, 855, 861
SsiI CCGC 2 cut(s) 257, 326
SspI AATATT 1 cut(s) 604
StyD4I CCNGG 3 cut(s) 82, 243, 542
TaaI ACNGT 5 cut(s) 10, 481, 524, 622, 797
TaiI ACGT 1 cut(s) 475
TaqI TCGA 1 cut(s) 96
TaqII GACCGA 1 cut(s) 690
TasI AATT 3 cut(s) 813, 855, 861
TatI WGTACW 2 cut(s) 519, 524
Tru1I TTAA 2 cut(s) 600, 816
Tru9I TTAA 2 cut(s) 600, 816
TscAI CASTG 2 cut(s) 13, 800
TseFI GTSAC 1 cut(s) 622
TseI GCWGC 4 cut(s) 183, 302, 364, 566
Tsp45I GTSAC 1 cut(s) 622
TspDTI ATGAA 3 cut(s) 458, 490, 854
TspRI CASTG 2 cut(s) 13, 800
Van91I CCANNNNNTGG 1 cut(s) 83
VneI GTGCAC 1 cut(s) 4
XapI RAATTY 2 cut(s) 855, 861
XceI RCATGY 2 cut(s) 323, 773
ZrmI AGTACT 1 cut(s) 521
Zsp2I ATGCAT 1 cut(s) 644
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.