MD08G1070400.v1.1

Peroxisome biogenesis factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0005456
Physical Location & Seq
Reverse (-)
5561816 .. 5565368
3553 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1070400.v1.1.491

Sequence Viewer

Length: 1191 bp
ATGGGTTGGAACGAGGCAACTGGGTCGGGGCAGCAAACCGGCCCGAGCGGGGGAGAACCAGGTCCAAGCAGCAGCAGCAGCAGCGACCCGGGCGCGGCACTGCGGTTCCCACCGGCAGCTCAGCCGGAGATCATGAGAGCCGCCGAAAAGGACGACCAGTACGCCTCCTTCGTCTACGACGCTTGCCGTGACGCCGTCCGCCACCTCTTGGGTACGAGAGTTTCTGTAGCTTACCAGAACGAGACGAAGCTTCTTGGGCAAGTGCTTTATTACGTTTTGACAACAGGGTCCGGCCAGCAAACACTTGGGGAAGAGTATTGCGACATCACTCAGGTTGCAGGACCTTATGGACTGCCTCCAACCCCGGCAAGGCGGGCACTTTTCATTGTGTACCAGACAGCTCTTCCTTATATTGCAGAACGAATTAGCTCTAGAGCTGCTAACCGTGGCATAGCCCTCGCTGATTCCGAGGAGCTCTATGGCCACAGTGCTTCATTGAGCAATGATGCTCAATCTTCTTCCACTATTCATGCTTCAACTGCCTCTACATCTGGGCCATCTGTTTCAGCTTTGAGAAGATTAAAAGAGAAGTTCAACGGGTTATGGATTTCGTTAGTCCGGCGATGGCCTACGGTGCTTCCTATAGCTCGAGAATTTTTGCAATTGGTTATTCGTGCTAACCTCATGTTCTTTTACTTTGAAGGCCTTTTCTATCATATATCAAAACGAACAGCAGGTATTCGTTATGTGTTCATCGGAAAACCATCTAACCAAAGGCCCAGATACCAAATTTTAGGCGTATTTCTATTGATTCAGCTTTGTATCATAGCTGCTGAAGGACTCAGGCGCAGCAATTTATCCTCTATTTCAAGTTCAGCTCATCAGACTTTTGGTACACACCAAACTTCTGCAGGTCGAGATTTGCCTGTGTTAAATGAGGAAGGTAATTTGATATCTGCGGATGCTGCCAAGGGAAGTTGGGTCTCTGAATCTTCAGCTTCAGAGGCCAGCGGGACCAGCAAATGCACACTCTGTTTGAGTAATCGCCAATACCCAACAGCCACACCTTGCGGTCACGTATTTTGCTGGAGCTGCGTTATGGAATGGTGCAACGAAAAGCCAGAATGCCCTCTCTGCCGGACTCCCATAACTCATTCGAGCCTAGTTTGCTTGTACCATTCAGACTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005778 GO:0005829 GO:0006082 GO:0006464 GO:0006513 GO:0006605 GO:0006625 GO:0006629 GO:0006631 GO:0006635 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009062 GO:0009790 GO:0009791 GO:0009793 GO:0009853 GO:0009987 GO:0010154 GO:0010381 GO:0015031 GO:0015833 GO:0015919 GO:0016020 GO:0016042 GO:0016043 GO:0016054 GO:0016558 GO:0016567 GO:0016740 GO:0017038 GO:0019395 GO:0019538 GO:0019752 GO:0019787 GO:0022406 GO:0022414 GO:0030258 GO:0031090 GO:0031903 GO:0032446 GO:0032501 GO:0032502 GO:0032787 GO:0033036 GO:0033365 GO:0034440 GO:0034613 GO:0036211 GO:0042579 GO:0042886 GO:0043094 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0043574 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044282 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0044743 GO:0045184 GO:0046395 GO:0046907 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051649 GO:0055085 GO:0055114 GO:0061458 GO:0065002 GO:0070647 GO:0070727 GO:0071702 GO:0071704 GO:0071705 GO:0071806 GO:0071840 GO:0072329 GO:0072594 GO:0072662 GO:0072663 GO:0098588 GO:0098805 GO:0140056 GO:0140096 GO:1901564 GO:1901575
KEGG Pathways
Metabolic & Signaling
ko04146 map04146
Pfam Domains
Protein Families
Pex2_Pex12 zf-C3HC4_3 zf-RING_2

Protein Analysis

397

Amino Acids

43.1

Weight (kDa)

7.95

Isoelectric Point (pI)

50.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pex2_Pex12 PF04757 51 - 282 3.3e-41 Pex2 / Pex12 amino terminal region
zf-C3HC4_3 PF13920 340 - 385 4.7e-10 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 341 - 380 6.4e-10 Ring finger domain
zf-C3HC4_2 PF13923 341 - 379 7e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 342 - 379 9.2e-07 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 286
Acc36I ACCTGC 2 cut(s) 725, 902
AccB7I CCANNNNNTGG 1 cut(s) 208
AccBSI CCGCTC 1 cut(s) 48
AccI GTMKAC 1 cut(s) 174
AccII CGCG 1 cut(s) 95
AciI CCGC 9 cut(s) 48, 95, 103, 141, 199, 373, 959, 1011, 1071
AcoI YGGCCR 2 cut(s) 292, 481
AcsI RAATTY 2 cut(s) 653, 789
AcuI CTGAAG 3 cut(s) 855, 978, 984
AcyI GRCGYC 1 cut(s) 192
AfaI GTAC 5 cut(s) 161, 214, 392, 895, 1175
AfiI CCNNNNNNNGG 5 cut(s) 49, 50, 94, 208, 369
AgsI TTSAA 4 cut(s) 537, 595, 701, 870
AjnI CCWGG 1 cut(s) 58
Alw21I GWGCWC 1 cut(s) 477
Alw26I GTCTC 2 cut(s) 236, 988
Ama87I CYCGRG 3 cut(s) 43, 88, 648
AoxI GGCC 8 cut(s) 40, 292, 481, 554, 626, 703, 776, 1005
ApoI RAATTY 2 cut(s) 653, 789
AspLEI GCGC 2 cut(s) 95, 849
AspS9I GGNCC 7 cut(s) 41, 62, 288, 341, 554, 777, 1014
AsuC2I CCSGG 3 cut(s) 89, 90, 365
AvaI CYCGRG 3 cut(s) 43, 88, 648
AvaII GGWCC 4 cut(s) 62, 288, 341, 1014
BaeGI GKGCMC 1 cut(s) 379
BaeI ACNNNNGTAYC 2 cut(s) 204, 237
BalI TGGCCA 1 cut(s) 483
BanII GRGCYC 1 cut(s) 477
BarI GAAGNNNNNNTAC 2 cut(s) 152, 184
Bbv12I GWGCWC 1 cut(s) 477
BccI CCATC 3 cut(s) 565, 618, 772
BceAI ACGGC 2 cut(s) 171, 179
BcgI CGANNNNNNTGC 4 cut(s) 6, 40, 439, 473
BciT130I CCWGG 1 cut(s) 60
BcnI CCSGG 3 cut(s) 89, 90, 365
BcoDI GTCTC 2 cut(s) 236, 988
BfaI CTAG 3 cut(s) 432, 1163, 1189
BfmI CTRYAG 3 cut(s) 225, 642, 909
BfuAI ACCTGC 2 cut(s) 725, 902
BlpI GCTNAGC 1 cut(s) 120
Bme1390I CCNGG 4 cut(s) 60, 89, 90, 365
Bme18I GGWCC 4 cut(s) 62, 288, 341, 1014
BmeT110I CYCGRG 3 cut(s) 43, 88, 648
BmgT120I GGNCC 7 cut(s) 41, 62, 288, 341, 554, 777, 1014
BmiI GGNNCC 3 cut(s) 107, 289, 1015
BmrFI CCNGG 4 cut(s) 60, 89, 90, 365
BmrI ACTGGG 1 cut(s) 30
BmsI GCATC 2 cut(s) 496, 952
BmuI ACTGGG 1 cut(s) 30
BpmI CTGGAG 1 cut(s) 1108
Bpu1102I GCTNAGC 1 cut(s) 120
BpuMI CCSGG 3 cut(s) 89, 90, 365
BsaAI YACGTR 1 cut(s) 1078
BsaHI GRCGYC 1 cut(s) 192
BsaI GGTCTC 1 cut(s) 988
BsaJI CCNNGG 5 cut(s) 88, 363, 445, 468, 969
Bsc4I CCNNNNNNNGG 5 cut(s) 49, 50, 94, 208, 369
Bse118I RCCGGY 2 cut(s) 38, 112
Bse1I ACTGG 2 cut(s) 25, 157
Bse3DI GCAATG 1 cut(s) 508
BseBI CCWGG 1 cut(s) 60
BseDI CCNNGG 5 cut(s) 88, 363, 445, 468, 969
BseGI GGATG 1 cut(s) 967
BseLI CCNNNNNNNGG 5 cut(s) 49, 50, 94, 208, 369
BseMI GCAATG 1 cut(s) 508
BseMII CTCAG 3 cut(s) 134, 344, 856
BseNI ACTGG 2 cut(s) 25, 157
BseRI GAGGAG 1 cut(s) 485
BseSI GKGCMC 1 cut(s) 379
Bsh1236I CGCG 1 cut(s) 95
BshFI GGCC 8 cut(s) 42, 294, 483, 556, 628, 705, 778, 1007
BsiHKAI GWGCWC 1 cut(s) 477
BsiHKCI CYCGRG 3 cut(s) 43, 88, 648
BsiSI CCGG 8 cut(s) 39, 89, 113, 125, 291, 365, 619, 1138
BslFI GGGAC 1 cut(s) 1027
BslI CCNNNNNNNGG 5 cut(s) 49, 50, 94, 208, 369
BsmAI GTCTC 2 cut(s) 236, 988
BsmBI CGTCTC 1 cut(s) 236
BsmFI GGGAC 1 cut(s) 1027
BsmI GAATGC 1 cut(s) 1130
BsnI GGCC 8 cut(s) 42, 294, 483, 556, 628, 705, 778, 1007
Bso31I GGTCTC 1 cut(s) 988
BsoBI CYCGRG 3 cut(s) 43, 88, 648
Bsp1286I GDGCHC 2 cut(s) 379, 477
Bsp143I GATC 1 cut(s) 129
Bsp1720I GCTNAGC 1 cut(s) 120
BspACI CCGC 9 cut(s) 48, 95, 103, 141, 199, 373, 959, 1011, 1071
BspANI GGCC 8 cut(s) 42, 294, 483, 556, 628, 705, 778, 1007
BspCNI CTCAG 3 cut(s) 133, 343, 855
BspFNI CGCG 1 cut(s) 95
BspHI TCATGA 1 cut(s) 132
BspLI GGNNCC 3 cut(s) 107, 289, 1015
BspMAI CTGCAG 1 cut(s) 913
BspMI ACCTGC 2 cut(s) 725, 902
BspQI GCTCTTC 1 cut(s) 408
BspTNI GGTCTC 1 cut(s) 988
BsrBI CCGCTC 1 cut(s) 48
BsrDI GCAATG 1 cut(s) 508
BsrFI RCCGGY 2 cut(s) 38, 112
BsrI ACTGG 2 cut(s) 25, 157
BssAI RCCGGY 2 cut(s) 38, 112
BssECI CCNNGG 5 cut(s) 88, 363, 445, 468, 969
BssMI GATC 1 cut(s) 129
BssNI GRCGYC 1 cut(s) 192
BssT1I CCWWGG 1 cut(s) 969
Bst2UI CCWGG 1 cut(s) 60
Bst4CI ACNGT 3 cut(s) 446, 488, 634
Bst6I CTCTTC 2 cut(s) 306, 408
BstACI GRCGYC 1 cut(s) 192
BstBAI YACGTR 1 cut(s) 1078
BstC8I GCNNGC 4 cut(s) 184, 296, 375, 1009
BstDEI CTNAG 3 cut(s) 120, 330, 842
BstDSI CCRYGG 1 cut(s) 445
BstF5I GGATG 1 cut(s) 967
BstFNI CGCG 1 cut(s) 95
BstHHI GCGC 2 cut(s) 95, 849
BstKTI GATC 1 cut(s) 132
BstMAI GTCTC 2 cut(s) 236, 988
BstMBI GATC 1 cut(s) 129
BstNI CCWGG 1 cut(s) 60
BstSCI CCNGG 4 cut(s) 58, 87, 88, 363
BstSFI CTRYAG 3 cut(s) 225, 642, 909
BstSLI GKGCMC 1 cut(s) 379
BstUI CGCG 1 cut(s) 95
BsuRI GGCC 8 cut(s) 42, 294, 483, 556, 628, 705, 778, 1007
BtgI CCRYGG 1 cut(s) 445
BtgZI GCGATG 1 cut(s) 637
BtsCI GGATG 1 cut(s) 967
BtsI GCAGTG 1 cut(s) 98
BtsIMutI CAGTG 2 cut(s) 98, 493
BveI ACCTGC 2 cut(s) 725, 902
Cac8I GCNNGC 4 cut(s) 184, 296, 375, 1009
CciI TCATGA 1 cut(s) 132
CfoI GCGC 2 cut(s) 95, 849
Cfr10I RCCGGY 2 cut(s) 38, 112
Cfr13I GGNCC 7 cut(s) 41, 62, 288, 341, 554, 777, 1014
Cfr9I CCCGGG 1 cut(s) 88
CseI GACGC 2 cut(s) 188, 200
CsiI ACCWGGT 1 cut(s) 58
Csp6I GTAC 5 cut(s) 160, 213, 391, 894, 1174
CviAII CATG 3 cut(s) 133, 530, 685
CviQI GTAC 5 cut(s) 160, 213, 391, 894, 1174
DdeI CTNAG 3 cut(s) 120, 330, 842
DpnI GATC 1 cut(s) 131
DpnII GATC 1 cut(s) 129
DrdI GACNNNNNNGTC 1 cut(s) 286
DseDI GACNNNNNNGTC 1 cut(s) 286
EaeI YGGCCR 2 cut(s) 292, 481
Eam1104I CTCTTC 2 cut(s) 306, 408
EarI CTCTTC 2 cut(s) 306, 408
EciI GGCGGA 1 cut(s) 188
Ecl136II GAGCTC 1 cut(s) 475
Eco130I CCWWGG 1 cut(s) 969
Eco147I AGGCCT 1 cut(s) 705
Eco24I GRGCYC 1 cut(s) 477
Eco31I GGTCTC 1 cut(s) 988
Eco32I GATATC 1 cut(s) 954
Eco47I GGWCC 4 cut(s) 62, 288, 341, 1014
Eco53kI GAGCTC 1 cut(s) 475
Eco57I CTGAAG 3 cut(s) 855, 978, 984
Eco88I CYCGRG 3 cut(s) 43, 88, 648
EcoICRI GAGCTC 1 cut(s) 475
EcoO109I RGGNCCY 1 cut(s) 341
EcoRII CCWGG 1 cut(s) 58
EcoRV GATATC 1 cut(s) 954
EcoT14I CCWWGG 1 cut(s) 969
EcoT38I GRGCYC 1 cut(s) 477
ErhI CCWWGG 1 cut(s) 969
Esp3I CGTCTC 1 cut(s) 236
FaeI CATG 3 cut(s) 136, 533, 688
FaqI GGGAC 1 cut(s) 1027
FatI CATG 3 cut(s) 132, 529, 684
FauI CCCGC 3 cut(s) 41, 366, 1004
FblI GTMKAC 1 cut(s) 174
FokI GGATG 1 cut(s) 974
FriOI GRGCYC 1 cut(s) 477
FspBI CTAG 3 cut(s) 432, 1163, 1189
GlaI GCGC 2 cut(s) 94, 848
GsuI CTGGAG 1 cut(s) 1108
HaeIII GGCC 8 cut(s) 42, 294, 483, 556, 628, 705, 778, 1007
HapII CCGG 8 cut(s) 39, 89, 113, 125, 291, 365, 619, 1138
HgaI GACGC 2 cut(s) 188, 200
HhaI GCGC 2 cut(s) 95, 849
Hin1I GRCGYC 1 cut(s) 192
Hin1II CATG 3 cut(s) 136, 533, 688
Hin6I GCGC 2 cut(s) 93, 847
HinP1I GCGC 2 cut(s) 93, 847
HindIII AAGCTT 1 cut(s) 248
HinfI GANTC 5 cut(s) 464, 811, 840, 989, 1141
HpaII CCGG 8 cut(s) 39, 89, 113, 125, 291, 365, 619, 1138
Hpy166II GTNNAC 3 cut(s) 175, 391, 896
Hpy188I TCNGA 6 cut(s) 469, 758, 885, 988, 1003, 1183
Hpy188III TCNNGA 4 cut(s) 133, 432, 650, 917
Hpy8I GTNNAC 3 cut(s) 175, 391, 896
Hpy99I CGWCG 1 cut(s) 182
HpyAV CCTTC 4 cut(s) 178, 695, 830, 935
HpyCH4III ACNGT 3 cut(s) 446, 488, 634
HpyCH4IV ACGT 2 cut(s) 273, 1077
HpyCH4V TGCA 6 cut(s) 338, 416, 661, 911, 1026, 1110
HpyF3I CTNAG 3 cut(s) 120, 330, 842
HpySE526I ACGT 2 cut(s) 273, 1077
Hsp92I GRCGYC 1 cut(s) 192
Hsp92II CATG 3 cut(s) 136, 533, 688
HspAI GCGC 2 cut(s) 93, 847
Kzo9I GATC 1 cut(s) 129
LguI GCTCTTC 1 cut(s) 408
LmnI GCTCC 2 cut(s) 472, 1089
LweI GCATC 2 cut(s) 496, 952
MabI ACCWGGT 1 cut(s) 58
MaeI CTAG 3 cut(s) 432, 1163, 1189
MaeII ACGT 2 cut(s) 273, 1077
MaeIII GTNAC 2 cut(s) 188, 1073
MalI GATC 1 cut(s) 131
MbiI CCGCTC 1 cut(s) 48
MboI GATC 1 cut(s) 129
MboII GAAGA 6 cut(s) 323, 395, 507, 510, 588, 984
MfeI CAATTG 1 cut(s) 662
MhlI GDGCHC 2 cut(s) 379, 477
MlsI TGGCCA 1 cut(s) 483
MluCI AATT 6 cut(s) 423, 653, 662, 789, 853, 946
MluNI TGGCCA 1 cut(s) 483
MlyI GAGTC 2 cut(s) 834, 1135
MmeI TCCRAC 1 cut(s) 383
Mox20I TGGCCA 1 cut(s) 483
MscI TGGCCA 1 cut(s) 483
MseI TTAA 2 cut(s) 581, 932
Msp20I TGGCCA 1 cut(s) 483
MspA1I CMGCKG 1 cut(s) 1011
MspI CCGG 8 cut(s) 39, 89, 113, 125, 291, 365, 619, 1138
MspR9I CCNGG 4 cut(s) 60, 89, 90, 365
MunI CAATTG 1 cut(s) 662
Mva1269I GAATGC 1 cut(s) 1130
MvaI CCWGG 1 cut(s) 60
MvnI CGCG 1 cut(s) 95
NciI CCSGG 3 cut(s) 89, 90, 365
NdeII GATC 1 cut(s) 129
NlaIII CATG 3 cut(s) 136, 533, 688
NlaIV GGNNCC 3 cut(s) 107, 289, 1015
NmuCI GTSAC 2 cut(s) 188, 1073
PaeR7I CTCGAG 1 cut(s) 648
PagI TCATGA 1 cut(s) 132
PceI AGGCCT 1 cut(s) 705
PciSI GCTCTTC 1 cut(s) 408
PcsI WCGNNNNNNNCGW 2 cut(s) 168, 465
PctI GAATGC 1 cut(s) 1130
PfeI GAWTC 3 cut(s) 464, 811, 989
PflFI GACNNNGTC 1 cut(s) 194
PflMI CCANNNNNTGG 1 cut(s) 208
PleI GAGTC 2 cut(s) 834, 1135
PpsI GAGTC 2 cut(s) 834, 1135
Ppu21I YACGTR 1 cut(s) 1078
PpuMI RGGWCCY 1 cut(s) 341
Psp124BI GAGCTC 1 cut(s) 477
Psp5II RGGWCCY 1 cut(s) 341
Psp6I CCWGG 1 cut(s) 58
PspGI CCWGG 1 cut(s) 58
PspN4I GGNNCC 3 cut(s) 107, 289, 1015
PspPI GGNCC 7 cut(s) 41, 62, 288, 341, 554, 777, 1014
PspPPI RGGWCCY 1 cut(s) 341
PstI CTGCAG 1 cut(s) 913
PsyI GACNNNGTC 1 cut(s) 194
RsaI GTAC 5 cut(s) 161, 214, 392, 895, 1175
RsaNI GTAC 5 cut(s) 160, 213, 391, 894, 1174
SacI GAGCTC 1 cut(s) 477
SapI GCTCTTC 1 cut(s) 408
SaqAI TTAA 2 cut(s) 581, 932
Sau3AI GATC 1 cut(s) 129
Sau96I GGNCC 7 cut(s) 41, 62, 288, 341, 554, 777, 1014
SchI GAGTC 2 cut(s) 834, 1135
ScrFI CCNGG 4 cut(s) 60, 89, 90, 365
SduI GDGCHC 2 cut(s) 379, 477
SexAI ACCWGGT 1 cut(s) 58
SfaNI GCATC 2 cut(s) 496, 952
SfcI CTRYAG 3 cut(s) 225, 642, 909
Sfr274I CTCGAG 1 cut(s) 648
SinI GGWCC 4 cut(s) 62, 288, 341, 1014
SlaI CTCGAG 1 cut(s) 648
SmaI CCCGGG 1 cut(s) 90
SmlI CTYRAG 1 cut(s) 648
SmoI CTYRAG 1 cut(s) 648
Sse9I AATT 6 cut(s) 423, 653, 662, 789, 853, 946
SseBI AGGCCT 1 cut(s) 705
SsiI CCGC 9 cut(s) 48, 95, 103, 141, 199, 373, 959, 1011, 1071
SspMI CTAG 3 cut(s) 432, 1163, 1189
SstI GAGCTC 1 cut(s) 477
StuI AGGCCT 1 cut(s) 705
StyD4I CCNGG 4 cut(s) 58, 87, 88, 363
StyI CCWWGG 1 cut(s) 969
TaaI ACNGT 3 cut(s) 446, 488, 634
TaiI ACGT 2 cut(s) 276, 1080
TaqI TCGA 3 cut(s) 649, 916, 1157
TasI AATT 6 cut(s) 423, 653, 662, 789, 853, 946
TauI GCSGC 2 cut(s) 98, 143
TfiI GAWTC 3 cut(s) 464, 811, 989
Tru1I TTAA 2 cut(s) 581, 932
Tru9I TTAA 2 cut(s) 581, 932
TscAI CASTG 2 cut(s) 105, 493
TseFI GTSAC 2 cut(s) 188, 1073
Tsp45I GTSAC 2 cut(s) 188, 1073
TspDTI ATGAA 4 cut(s) 373, 483, 518, 742
TspMI CCCGGG 1 cut(s) 88
TspRI CASTG 2 cut(s) 105, 493
Tth111I GACNNNGTC 1 cut(s) 194
Van91I CCANNNNNTGG 1 cut(s) 208
VpaK11BI GGWCC 4 cut(s) 62, 288, 341, 1014
XapI RAATTY 2 cut(s) 653, 789
XbaI TCTAGA 1 cut(s) 431
XcmI CCANNNNNNNNNTGG 1 cut(s) 302
XhoI CTCGAG 1 cut(s) 648
XmaI CCCGGG 1 cut(s) 88
XmiI GTMKAC 1 cut(s) 174
XspI CTAG 3 cut(s) 432, 1163, 1189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.