MD15G1051800.v1.1

Peroxisome biogenesis factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0005456
Physical Location & Seq
Forward (+)
3560185 .. 3563544
3360 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1051800.v1.1.491

Sequence Viewer

Length: 1191 bp
ATGGGTTGGAACGAGGCAACCGGATCGGGGCAGCAAGCCGGCCCGAGCGGAGGAGAACCGGGTCCCAGCAGCAGCAGCGACCCGGGCGTGGCACGGCAGTTCCCACTTGCGGCTCAGCCGGAGATCATGAGAGCAGCCGAAAAGGACGACCAGTACGCCTCCTTCGTCTACGACGCTTGCCGAGACGCCGTCCGCCACCTCTTGGGTACGAGAGTTTCTGTAGCTTACCAGAACGAGACAAAGCTTCTTGGGCAAGTGCTTTATTACGTTTTGACAACAGGGTCCGGCCAGCAAACTCTCGGGGAAGAGTACTGCGACATCACTCAGGTTGCCGGACCTTATGGACTGCCTCCAATGCCAGCAAGGCGTGTGCTTTTCATTGTCTACCAGACAGCCCTTCCTTACATTGCCGAACGAATTAGCTCTAGAGCTGCTAACCGTGGCATAGCCCTAGCTGATTCCGAAGAGCTCTATGGCATCAGTGCTTCACTAAGCAATGATGCTCAATCTTCTTCCACTATTCATAATTCAGCTGGCTCTACATCTGGTCCATCTGTTTCAGCTTTGAGAAGATTAAAAGAGAAGTTCAACGGGTTATGGATTTCGTTAGCTCGGAGATGGCCTACGGTGCTTCCTATAGCTCGAGAATTTTTGCAATTGGTTATTCGTGCTAACCTCATGTTCTTTTACTTTGAAGGCCTCTTCTATCATATATCAAAACGAGCAGCAGGCATTCGTTATGTGTTCATCGGAAAACCATCTAACCAAAGGCCTCGATATCAAATTTTAGGCGTATTTCTATTGATTCAGCTTTGTATCATAGCTGCTGAAGGACTGAGGCGCAGCAATTTATCCTCTATTGCAAGTTCAGCTCAGCAGACTTTTGGTACGCACCAAACTTCTGCAGGTCGAGATTTGCCTGTGTTAAATGAGGAAGGTAATTTGATATCTGCGGACGCTACCAGGGGAAGTTGGGTCTCTGAATCTTCGACTTCAGAGTCTCAGGCCAGCGGGAGCAGCAAATGCACACTCTGTTTGAGTAATCGCCACTACCCAACAGCCACACCTTGCGGTCACGTATTTTGCTGGAGCTGCATCATGGAATGGTGCAACGAAAAGCCCGAATGCCCCCTCTGCCGGACTCCCATAACCCATTCGAGCCTAGTTTGCTTGTACCATTCAGACTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005778 GO:0005829 GO:0006082 GO:0006464 GO:0006513 GO:0006605 GO:0006625 GO:0006629 GO:0006631 GO:0006635 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009062 GO:0009790 GO:0009791 GO:0009793 GO:0009853 GO:0009987 GO:0010154 GO:0010381 GO:0015031 GO:0015833 GO:0015919 GO:0016020 GO:0016042 GO:0016043 GO:0016054 GO:0016558 GO:0016567 GO:0016740 GO:0017038 GO:0019395 GO:0019538 GO:0019752 GO:0019787 GO:0022406 GO:0022414 GO:0030258 GO:0031090 GO:0031903 GO:0032446 GO:0032501 GO:0032502 GO:0032787 GO:0033036 GO:0033365 GO:0034440 GO:0034613 GO:0036211 GO:0042579 GO:0042886 GO:0043094 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0043574 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044282 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0044743 GO:0045184 GO:0046395 GO:0046907 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051649 GO:0055085 GO:0055114 GO:0061458 GO:0065002 GO:0070647 GO:0070727 GO:0071702 GO:0071704 GO:0071705 GO:0071806 GO:0071840 GO:0072329 GO:0072594 GO:0072662 GO:0072663 GO:0098588 GO:0098805 GO:0140056 GO:0140096 GO:1901564 GO:1901575
KEGG Pathways
Metabolic & Signaling
ko04146 map04146
Pfam Domains
Protein Families
Pex2_Pex12 zf-C3HC4_3 zf-RING_2

Protein Analysis

397

Amino Acids

43.22

Weight (kDa)

7.95

Isoelectric Point (pI)

53.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pex2_Pex12 PF04757 49 - 280 4.7e-41 Pex2 / Pex12 amino terminal region
zf-C3HC4_3 PF13920 340 - 385 7.9e-10 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 341 - 380 1.7e-09 Ring finger domain
zf-C3HC4_2 PF13923 341 - 379 5.9e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 342 - 379 9.3e-07 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 280, 997
Acc36I ACCTGC 1 cut(s) 896
AccB7I CCANNNNNTGG 1 cut(s) 202
AccBSI CCGCTC 1 cut(s) 48
AccI GTMKAC 2 cut(s) 168, 384
AciI CCGC 6 cut(s) 48, 110, 193, 953, 1011, 1071
AclWI GGATC 1 cut(s) 31
AcoI YGGCCR 1 cut(s) 286
AcsI RAATTY 2 cut(s) 647, 783
AcuI CTGAAG 2 cut(s) 849, 978
AcyI GRCGYC 1 cut(s) 186
AfaI GTAC 5 cut(s) 155, 208, 311, 889, 1175
AfiI CCNNNNNNNGG 6 cut(s) 27, 50, 88, 109, 202, 1137
AgsI TTSAA 2 cut(s) 589, 695
AjnI CCWGG 1 cut(s) 962
Alw21I GWGCWC 1 cut(s) 471
Alw26I GTCTC 4 cut(s) 177, 230, 982, 1005
AlwI GGATC 1 cut(s) 31
Ama87I CYCGRG 4 cut(s) 43, 82, 299, 642
AoxI GGCC 6 cut(s) 40, 286, 620, 697, 770, 1005
ApoI RAATTY 2 cut(s) 647, 783
AspLEI GCGC 1 cut(s) 843
AspS9I GGNCC 5 cut(s) 41, 62, 282, 335, 548
AsuC2I CCSGG 3 cut(s) 60, 83, 84
AvaI CYCGRG 4 cut(s) 43, 82, 299, 642
AvaII GGWCC 4 cut(s) 62, 282, 335, 548
BaeI ACNNNNGTAYC 2 cut(s) 198, 231
BanII GRGCYC 1 cut(s) 471
BarI GAAGNNNNNNTAC 2 cut(s) 146, 178
Bbv12I GWGCWC 1 cut(s) 471
BccI CCATC 3 cut(s) 559, 612, 766
BceAI ACGGC 2 cut(s) 110, 173
BcgI CGANNNNNNTGC 2 cut(s) 6, 40
BciT130I CCWGG 1 cut(s) 964
BcnI CCSGG 3 cut(s) 60, 83, 84
BcoDI GTCTC 4 cut(s) 177, 230, 982, 1005
BfaI CTAG 4 cut(s) 426, 452, 1163, 1189
BfmI CTRYAG 3 cut(s) 219, 636, 903
BfuAI ACCTGC 1 cut(s) 896
BglI GCCNNNNNGGC 1 cut(s) 364
BlpI GCTNAGC 2 cut(s) 114, 873
BmcAI AGTACT 1 cut(s) 311
Bme1390I CCNGG 4 cut(s) 60, 83, 84, 964
Bme18I GGWCC 4 cut(s) 62, 282, 335, 548
BmeT110I CYCGRG 4 cut(s) 43, 82, 299, 642
BmgT120I GGNCC 5 cut(s) 41, 62, 282, 335, 548
BmiI GGNNCC 3 cut(s) 63, 64, 283
BmrFI CCNGG 4 cut(s) 60, 83, 84, 964
BmsI GCATC 3 cut(s) 486, 490, 1104
BpmI CTGGAG 1 cut(s) 1108
Bpu1102I GCTNAGC 2 cut(s) 114, 873
BpuMI CCSGG 3 cut(s) 60, 83, 84
BsaAI YACGTR 1 cut(s) 1078
BsaHI GRCGYC 1 cut(s) 186
BsaI GGTCTC 1 cut(s) 982
BsaJI CCNNGG 3 cut(s) 82, 439, 963
BsaWI WCCGGW 1 cut(s) 20
Bsc4I CCNNNNNNNGG 6 cut(s) 27, 50, 88, 109, 202, 1137
Bse118I RCCGGY 1 cut(s) 38
Bse1I ACTGG 1 cut(s) 151
Bse3DI GCAATG 2 cut(s) 405, 502
BseBI CCWGG 1 cut(s) 964
BseDI CCNNGG 3 cut(s) 82, 439, 963
BseLI CCNNNNNNNGG 6 cut(s) 27, 50, 88, 109, 202, 1137
BseMI GCAATG 2 cut(s) 405, 502
BseMII CTCAG 5 cut(s) 128, 338, 827, 887, 1016
BseNI ACTGG 1 cut(s) 151
BseRI GAGGAG 1 cut(s) 66
BseYI CCCAGC 1 cut(s) 65
BshFI GGCC 6 cut(s) 42, 288, 622, 699, 772, 1007
BsiHKAI GWGCWC 1 cut(s) 471
BsiHKCI CYCGRG 4 cut(s) 43, 82, 299, 642
BsiSI CCGG 8 cut(s) 21, 39, 59, 83, 119, 285, 333, 1138
BslFI GGGAC 1 cut(s) 48
BslI CCNNNNNNNGG 6 cut(s) 27, 50, 88, 109, 202, 1137
BsmAI GTCTC 4 cut(s) 177, 230, 982, 1005
BsmBI CGTCTC 1 cut(s) 177
BsmFI GGGAC 1 cut(s) 48
BsmI GAATGC 2 cut(s) 732, 1130
BsnI GGCC 6 cut(s) 42, 288, 622, 699, 772, 1007
Bso31I GGTCTC 1 cut(s) 982
BsoBI CYCGRG 4 cut(s) 43, 82, 299, 642
Bsp1286I GDGCHC 1 cut(s) 471
Bsp143I GATC 2 cut(s) 23, 123
Bsp1720I GCTNAGC 2 cut(s) 114, 873
BspACI CCGC 6 cut(s) 48, 110, 193, 953, 1011, 1071
BspANI GGCC 6 cut(s) 42, 288, 622, 699, 772, 1007
BspCNI CTCAG 5 cut(s) 127, 337, 828, 886, 1015
BspHI TCATGA 1 cut(s) 126
BspLI GGNNCC 3 cut(s) 63, 64, 283
BspMAI CTGCAG 1 cut(s) 907
BspMI ACCTGC 1 cut(s) 896
BspPI GGATC 1 cut(s) 31
BspQI GCTCTTC 1 cut(s) 459
BspTNI GGTCTC 1 cut(s) 982
BsrBI CCGCTC 1 cut(s) 48
BsrDI GCAATG 2 cut(s) 405, 502
BsrFI RCCGGY 1 cut(s) 38
BsrI ACTGG 1 cut(s) 151
BssAI RCCGGY 1 cut(s) 38
BssECI CCNNGG 3 cut(s) 82, 439, 963
BssMI GATC 2 cut(s) 23, 123
BssNI GRCGYC 1 cut(s) 186
Bst2UI CCWGG 1 cut(s) 964
Bst4CI ACNGT 2 cut(s) 440, 628
Bst6I CTCTTC 3 cut(s) 300, 459, 707
BstACI GRCGYC 1 cut(s) 186
BstAPI GCANNNNNTGC 1 cut(s) 1023
BstBAI YACGTR 1 cut(s) 1078
BstC8I GCNNGC 8 cut(s) 36, 40, 178, 290, 360, 535, 730, 1009
BstDEI CTNAG 6 cut(s) 114, 324, 491, 836, 873, 1002
BstDSI CCRYGG 1 cut(s) 439
BstHHI GCGC 1 cut(s) 843
BstKTI GATC 2 cut(s) 26, 126
BstMAI GTCTC 4 cut(s) 177, 230, 982, 1005
BstMBI GATC 2 cut(s) 23, 123
BstNI CCWGG 1 cut(s) 964
BstSCI CCNGG 4 cut(s) 58, 81, 82, 962
BstSFI CTRYAG 3 cut(s) 219, 636, 903
BsuRI GGCC 6 cut(s) 42, 288, 622, 699, 772, 1007
BtgI CCRYGG 1 cut(s) 439
BtsIMutI CAGTG 1 cut(s) 487
BveI ACCTGC 1 cut(s) 896
Cac8I GCNNGC 8 cut(s) 36, 40, 178, 290, 360, 535, 730, 1009
CciI TCATGA 1 cut(s) 126
CfoI GCGC 1 cut(s) 843
Cfr10I RCCGGY 1 cut(s) 38
Cfr13I GGNCC 5 cut(s) 41, 62, 282, 335, 548
Cfr9I CCCGGG 1 cut(s) 82
CseI GACGC 3 cut(s) 182, 194, 965
Csp6I GTAC 5 cut(s) 154, 207, 310, 888, 1174
CviAII CATG 3 cut(s) 127, 679, 1099
CviQI GTAC 5 cut(s) 154, 207, 310, 888, 1174
DdeI CTNAG 6 cut(s) 114, 324, 491, 836, 873, 1002
DpnI GATC 2 cut(s) 25, 125
DpnII GATC 2 cut(s) 23, 123
DrdI GACNNNNNNGTC 2 cut(s) 280, 997
DseDI GACNNNNNNGTC 2 cut(s) 280, 997
EaeI YGGCCR 1 cut(s) 286
Eam1104I CTCTTC 3 cut(s) 300, 459, 707
EarI CTCTTC 3 cut(s) 300, 459, 707
EciI GGCGGA 1 cut(s) 182
Ecl136II GAGCTC 1 cut(s) 469
Eco147I AGGCCT 2 cut(s) 699, 772
Eco24I GRGCYC 1 cut(s) 471
Eco31I GGTCTC 1 cut(s) 982
Eco32I GATATC 2 cut(s) 779, 948
Eco47I GGWCC 4 cut(s) 62, 282, 335, 548
Eco53kI GAGCTC 1 cut(s) 469
Eco57I CTGAAG 2 cut(s) 849, 978
Eco88I CYCGRG 4 cut(s) 43, 82, 299, 642
EcoICRI GAGCTC 1 cut(s) 469
EcoO109I RGGNCCY 1 cut(s) 62
EcoRII CCWGG 1 cut(s) 962
EcoRV GATATC 2 cut(s) 779, 948
EcoT38I GRGCYC 1 cut(s) 471
Esp3I CGTCTC 1 cut(s) 177
FaeI CATG 3 cut(s) 130, 682, 1102
FaqI GGGAC 1 cut(s) 48
FatI CATG 3 cut(s) 126, 678, 1098
FauI CCCGC 1 cut(s) 1004
FblI GTMKAC 2 cut(s) 168, 384
FriOI GRGCYC 1 cut(s) 471
FspBI CTAG 4 cut(s) 426, 452, 1163, 1189
GlaI GCGC 1 cut(s) 842
GsaI CCCAGC 1 cut(s) 69
GsuI CTGGAG 1 cut(s) 1108
HaeIII GGCC 6 cut(s) 42, 288, 622, 699, 772, 1007
HapII CCGG 8 cut(s) 21, 39, 59, 83, 119, 285, 333, 1138
HgaI GACGC 3 cut(s) 182, 194, 965
HhaI GCGC 1 cut(s) 843
Hin1I GRCGYC 1 cut(s) 186
Hin1II CATG 3 cut(s) 130, 682, 1102
Hin6I GCGC 1 cut(s) 841
HinP1I GCGC 1 cut(s) 841
HindIII AAGCTT 1 cut(s) 242
HinfI GANTC 5 cut(s) 458, 805, 983, 998, 1141
HpaII CCGG 8 cut(s) 21, 39, 59, 83, 119, 285, 333, 1138
Hpy166II GTNNAC 2 cut(s) 169, 385
Hpy188I TCNGA 6 cut(s) 463, 615, 752, 982, 997, 1183
Hpy188III TCNNGA 4 cut(s) 127, 426, 644, 911
Hpy8I GTNNAC 2 cut(s) 169, 385
Hpy99I CGWCG 1 cut(s) 176
HpyAV CCTTC 5 cut(s) 172, 407, 689, 824, 929
HpyCH4III ACNGT 2 cut(s) 440, 628
HpyCH4IV ACGT 2 cut(s) 267, 1077
HpyCH4V TGCA 6 cut(s) 655, 863, 905, 1026, 1095, 1110
HpyF3I CTNAG 6 cut(s) 114, 324, 491, 836, 873, 1002
HpySE526I ACGT 2 cut(s) 267, 1077
Hsp92I GRCGYC 1 cut(s) 186
Hsp92II CATG 3 cut(s) 130, 682, 1102
HspAI GCGC 1 cut(s) 841
KflI GGGWCCC 1 cut(s) 62
KroI GCCGGC 1 cut(s) 38
KroNI GCCGGC 1 cut(s) 40
Kzo9I GATC 2 cut(s) 23, 123
LguI GCTCTTC 1 cut(s) 459
LmnI GCTCC 2 cut(s) 1014, 1089
LweI GCATC 3 cut(s) 486, 490, 1104
MaeI CTAG 4 cut(s) 426, 452, 1163, 1189
MaeII ACGT 2 cut(s) 267, 1077
MaeIII GTNAC 1 cut(s) 1073
MalI GATC 2 cut(s) 25, 125
MbiI CCGCTC 1 cut(s) 48
MboI GATC 2 cut(s) 23, 123
MboII GAAGA 7 cut(s) 317, 476, 501, 504, 582, 694, 978
MfeI CAATTG 1 cut(s) 656
MhlI GDGCHC 1 cut(s) 471
MluCI AATT 7 cut(s) 417, 526, 647, 656, 783, 847, 940
MlyI GAGTC 2 cut(s) 1007, 1135
MroNI GCCGGC 1 cut(s) 38
MseI TTAA 2 cut(s) 575, 926
MspA1I CMGCKG 2 cut(s) 533, 1011
MspI CCGG 8 cut(s) 21, 39, 59, 83, 119, 285, 333, 1138
MspR9I CCNGG 4 cut(s) 60, 83, 84, 964
MunI CAATTG 1 cut(s) 656
Mva1269I GAATGC 2 cut(s) 732, 1130
MvaI CCWGG 1 cut(s) 964
NaeI GCCGGC 1 cut(s) 40
NciI CCSGG 3 cut(s) 60, 83, 84
NdeII GATC 2 cut(s) 23, 123
NgoMIV GCCGGC 1 cut(s) 38
NlaIII CATG 3 cut(s) 130, 682, 1102
NlaIV GGNNCC 3 cut(s) 63, 64, 283
NmeAIII GCCGAG 1 cut(s) 206
NmuCI GTSAC 1 cut(s) 1073
PaeR7I CTCGAG 1 cut(s) 642
PagI TCATGA 1 cut(s) 126
PceI AGGCCT 2 cut(s) 699, 772
PciSI GCTCTTC 1 cut(s) 459
PcsI WCGNNNNNNNCGW 2 cut(s) 162, 1119
PctI GAATGC 2 cut(s) 732, 1130
PdiI GCCGGC 1 cut(s) 40
PfeI GAWTC 3 cut(s) 458, 805, 983
PflFI GACNNNGTC 1 cut(s) 188
PflMI CCANNNNNTGG 1 cut(s) 202
PleI GAGTC 2 cut(s) 1006, 1135
PpsI GAGTC 2 cut(s) 1006, 1135
Ppu21I YACGTR 1 cut(s) 1078
PpuMI RGGWCCY 1 cut(s) 62
Psp124BI GAGCTC 1 cut(s) 471
Psp5II RGGWCCY 1 cut(s) 62
Psp6I CCWGG 1 cut(s) 962
PspFI CCCAGC 1 cut(s) 65
PspGI CCWGG 1 cut(s) 962
PspN4I GGNNCC 3 cut(s) 63, 64, 283
PspPI GGNCC 5 cut(s) 41, 62, 282, 335, 548
PspPPI RGGWCCY 1 cut(s) 62
PstI CTGCAG 1 cut(s) 907
PsyI GACNNNGTC 1 cut(s) 188
PvuII CAGCTG 1 cut(s) 533
RsaI GTAC 5 cut(s) 155, 208, 311, 889, 1175
RsaNI GTAC 5 cut(s) 154, 207, 310, 888, 1174
SacI GAGCTC 1 cut(s) 471
SapI GCTCTTC 1 cut(s) 459
SaqAI TTAA 2 cut(s) 575, 926
Sau3AI GATC 2 cut(s) 23, 123
Sau96I GGNCC 5 cut(s) 41, 62, 282, 335, 548
ScaI AGTACT 1 cut(s) 311
SchI GAGTC 2 cut(s) 1007, 1135
ScrFI CCNGG 4 cut(s) 60, 83, 84, 964
SduI GDGCHC 1 cut(s) 471
SfaNI GCATC 3 cut(s) 486, 490, 1104
SfcI CTRYAG 3 cut(s) 219, 636, 903
Sfr274I CTCGAG 1 cut(s) 642
SinI GGWCC 4 cut(s) 62, 282, 335, 548
SlaI CTCGAG 1 cut(s) 642
SmaI CCCGGG 1 cut(s) 84
SmlI CTYRAG 1 cut(s) 642
SmoI CTYRAG 1 cut(s) 642
Sse9I AATT 7 cut(s) 417, 526, 647, 656, 783, 847, 940
SseBI AGGCCT 2 cut(s) 699, 772
SsiI CCGC 6 cut(s) 48, 110, 193, 953, 1011, 1071
SspMI CTAG 4 cut(s) 426, 452, 1163, 1189
SstI GAGCTC 1 cut(s) 471
StuI AGGCCT 2 cut(s) 699, 772
StyD4I CCNGG 4 cut(s) 58, 81, 82, 962
TaaI ACNGT 2 cut(s) 440, 628
TaiI ACGT 2 cut(s) 270, 1080
TaqI TCGA 5 cut(s) 643, 775, 910, 989, 1157
TasI AATT 7 cut(s) 417, 526, 647, 656, 783, 847, 940
TatI WGTACW 1 cut(s) 309
TauI GCSGC 1 cut(s) 113
TfiI GAWTC 3 cut(s) 458, 805, 983
Tru1I TTAA 2 cut(s) 575, 926
Tru9I TTAA 2 cut(s) 575, 926
TscAI CASTG 1 cut(s) 487
TseFI GTSAC 1 cut(s) 1073
Tsp45I GTSAC 1 cut(s) 1073
TspDTI ATGAA 3 cut(s) 367, 512, 736
TspMI CCCGGG 1 cut(s) 82
TspRI CASTG 1 cut(s) 487
Tth111I GACNNNGTC 1 cut(s) 188
Van91I CCANNNNNTGG 1 cut(s) 202
VpaK11BI GGWCC 4 cut(s) 62, 282, 335, 548
XapI RAATTY 2 cut(s) 647, 783
XbaI TCTAGA 1 cut(s) 425
XhoI CTCGAG 1 cut(s) 642
XmaI CCCGGG 1 cut(s) 82
XmiI GTMKAC 2 cut(s) 168, 384
XspI CTAG 4 cut(s) 426, 452, 1163, 1189
ZrmI AGTACT 1 cut(s) 311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.