Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0013937

Physical Location & Seq

Genomic Coordinates Reverse (-)

7413451 .. 7416601

Gene Structure 3151 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1089600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1608 bp

ATGAGCGCCCAATTGCGAGAAGACGACGACGAGTCGTCGAAGCTGCGAGACGACGCGGACGATGACGAAGCCAACCGCCTGCTGCAGCCGCAGTCCCTGTCGCTGTCGTCGTCGACATGGGACGACGACGAGGAGGTGGCGTTCGAGGCGCGGGAGAAGATCCTGATCGTCGACGATGCCGTCGACGACGAATCCACGGCGGTCCCGCCTTTTTCGTGGAAGAAGCTGTGGCTGTTCACCGGACCGGGTTTCCTGATGAGCATAGCGTTCCTGGATCCGGGCAATTTAGAAGGGGATCTCCAGTCGGGCGCGATTGCCGGTTACTCGCTGCTATGGCTTTTAATGTGGGCCACCGCCATGGGACTGCTGGTCCAGCTGCTCTCTGCCCGGGTCGGGGTCGCGACGGGTCGGCATTTGGCGGAGCTCTGTAGGGAGGAGTATCCGAAATGGGCCGTGTTGGTGCTCTGGTTCATGGCGGAGCTAGCGTTGATTGGGGCGGATATTCAGGAAGTGATTGGGAGCGCCATTGCGATTCAGATACTGAGCCATGGGTTCTTGCCGCTCTGGGCTGGAGTTCTGATTACGGCTTTGGATTGTTTCATCTTTTTATTTCTTGAGAACTATGGGGTGAGGAAGTTGGAAGGTGTGTTTGCAGTTCTCATAGCAACTATGGGTTTATCGTTTGCCTGGATGTTTGTTGACACAAAACCGAGTGGGAAAGAACTTTTAATGGGTATTGTGGTTCCAAGACTTAGCTCGAAAACAATTCGGCAGGCGGTAGGAGTTGTGGGTTGTGTCATTATGCCACACAACGTATTCTTGCATTCTGCTTTGGTACAGTCAAGAAATATTGATCCGAACAAGAAAGGACGGGTTCAAGAGGCACTAAACTACTACTCAATTGAGTCATCAATTGCACTTTTGATCTCCTTGATGATCAATTTGTTTGTCACCACAGTTTTCGCCAAGGGATTTTATGGTACTAAACAAGCAGAAAGTATAGGACTGGTAAATGCAGGTCAGTATCTTCAGGAGAAGTATGGAGGAGGGCTTTATCCGATTCTTTATATCTGGGGTATTGGGTTGCTGGCGGCTGGACAAAGTAGCACAATAACTGGAACGTATGCTGGGCAGTTTATAATGGGAGGCTTCCTTAATCTTCGTTTGAAGAAGTGGCTGAGGGCATTGATCACGCGGAGTTTTGCAATTGTGCCAACTATAGTTGTAGCAATTATCTTTAATAGATCTGAGGCTTCGTTGGATGTTTTGAATGAATGGCTTAATGTGCTTCAGTCGATTCAGATTCCTTTTGCCCTTATACCACTTCTTACCTTGGTGTCCAAGGAGCAGGTCATGGGGGTCTTCAAGATTGGACCTGGTCTCGAGAAAGTGGCATGGACTGTGGCGGTTTTGGTGATGGTGATCAATGGTTATCTGTTGTTGGATTTCTTCATTTCTGAAGTCAGAGGAGTGTTGTTTGCTCTTGTGGTCTGCAGTGGCACAGCTGCGTATATAACATTTATTGTATATCTTGTTTCGCGGAGTGGTGCCCTCCCTCCTACTTTTTTCAGTTCACTGTTCAAGAGGTTTGCAGATTCAGGTAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000041 GO:0001558 GO:0003674 GO:0005215 GO:0005384 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005802 GO:0006810 GO:0006811 GO:0006812 GO:0006828 GO:0007275 GO:0008150 GO:0008324 GO:0009653 GO:0009888 GO:0009987 GO:0010015 GO:0010035 GO:0010038 GO:0010042 GO:0010053 GO:0010054 GO:0012505 GO:0015075 GO:0015318 GO:0016049 GO:0021700 GO:0022603 GO:0022604 GO:0022622 GO:0022857 GO:0022890 GO:0030001 GO:0030154 GO:0030307 GO:0031984 GO:0032501 GO:0032502 GO:0034220 GO:0040007 GO:0040008 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045927 GO:0046873 GO:0046915 GO:0048364 GO:0048468 GO:0048469 GO:0048518 GO:0048522 GO:0048588 GO:0048589 GO:0048638 GO:0048639 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051510 GO:0051512 GO:0051716 GO:0055085 GO:0060560 GO:0065007 GO:0070887 GO:0071241 GO:0071248 GO:0071287 GO:0071421 GO:0071695 GO:0080147 GO:0090558 GO:0090627 GO:0098655 GO:0098660 GO:0098662 GO:0098791 GO:0099402 GO:1905392

KEGG Pathways

Metabolic & Signaling

ko04142 ko04216 ko04978 map04142 map04216 map04978

Pfam Domains

Protein Families

Nramp

Protein Analysis

536

Amino Acids

58.71

Weight (kDa)

5.05

Isoelectric Point (pI)

36.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Nramp	PF01566	97 - 458	1.3e-113	Natural resistance-associated macrophage protein-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0013937)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G47240 AT4G18790
fragaria_vesca	FvH4_2g33450 FvH4_2g33450
malus_domestica	MD08G1089600.v1.1 MD15G1074200.v1.1
prunus_persica	Prupe.1G427000_v2.0.a1 Prupe.1G427000_v2.0.a1
pyrus_communis	pycom08g07290 pycom15g06940
rosa_chinensis	RchiOBHm_Chr6g0309081
rosa_laevigata	RLG00000010591
rosa_roxburghii	Rroxscaffold_7G00159660
rosa_rugosa	Rorug06G0369600
rosa_samantha	Rh6AG482300 Rh6CG496100
rosa_wichuraiana	Rw6G041990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1139
AasI	GACNNNNNNGTC	1 cut(s)	179
Acc36I	ACCTGC	2 cut(s)	1007, 1339
AccB1I	GGYRCC	1 cut(s)	1547
AccBSI	CCGCTC	1 cut(s)	562
AccI	GTMKAC	3 cut(s)	113, 171, 183
AccII	CGCG	6 cut(s)	56, 151, 311, 401, 1195, 1540
AclWI	GGATC	5 cut(s)	154, 269, 282, 303, 848
AcuI	CTGAAG	3 cut(s)	1013, 1274, 1479
AfaI	GTAC	2 cut(s)	837, 982
AfiI	CCNNNNNNNGG	3 cut(s)	277, 393, 394
AgsI	TTSAA	5 cut(s)	878, 1168, 1270, 1366, 1582
AhdI	GACNNNNNGTC	3 cut(s)	31, 34, 368
AjnI	CCWGG	3 cut(s)	270, 686, 1375
AloI	GAACNNNNNNTCC	2 cut(s)	125, 157
AluBI	AGCT	7 cut(s)	43, 226, 376, 424, 481, 756, 1505
AluI	AGCT	7 cut(s)	43, 226, 376, 424, 481, 756, 1505
Alw21I	GWGCWC	2 cut(s)	426, 465
Alw26I	GTCTC	2 cut(s)	42, 1385
AlwI	GGATC	5 cut(s)	154, 269, 282, 303, 848
AlwNI	CAGNNNCTG	2 cut(s)	97, 541
Ama87I	CYCGRG	2 cut(s)	387, 1382
AoxI	GGCC	2 cut(s)	348, 450
ApeKI	GCWGC	6 cut(s)	43, 82, 85, 328, 376, 1505
AspLEI	GCGC	4 cut(s)	8, 151, 311, 524
AspS9I	GGNCC	6 cut(s)	202, 242, 348, 370, 450, 1373
AsuC2I	CCSGG	4 cut(s)	246, 279, 388, 389
AsuHPI	GGTGA	5 cut(s)	229, 640, 943, 1426, 1432
AsuNHI	GCTAGC	1 cut(s)	481
AvaI	CYCGRG	2 cut(s)	387, 1382
AvaII	GGWCC	4 cut(s)	202, 242, 370, 1373
BaeGI	GKGCMC	1 cut(s)	1552
BamHI	GGATCC	1 cut(s)	274
BanI	GGYRCC	1 cut(s)	1547
BanII	GRGCYC	1 cut(s)	426
BbsI	GAAGAC	2 cut(s)	27, 1354
Bbv12I	GWGCWC	2 cut(s)	426, 465
BbvCI	CCTCAGC	1 cut(s)	1178
BbvI	GCAGC	6 cut(s)	30, 69, 97, 315, 363, 1492
BccI	CCATC	1 cut(s)	1411
BceAI	ACGGC	4 cut(s)	164, 213, 437, 600
BciT130I	CCWGG	3 cut(s)	272, 688, 1377
BciVI	GTATCC	1 cut(s)	450
BclI	TGATCA	3 cut(s)	936, 1188, 1422
BcnI	CCSGG	4 cut(s)	246, 279, 388, 389
BcoDI	GTCTC	2 cut(s)	42, 1385
BfaI	CTAG	1 cut(s)	482
BfmI	CTRYAG	4 cut(s)	83, 427, 1218, 1492
BfoI	RGCGCY	2 cut(s)	9, 525
BfuAI	ACCTGC	2 cut(s)	1007, 1339
BfuI	GTATCC	1 cut(s)	450
BglII	AGATCT	1 cut(s)	1244
BisI	GCNGC	9 cut(s)	44, 83, 86, 89, 329, 377, 560, 1092, 1506
BlsI	GCNGC	9 cut(s)	45, 84, 87, 90, 330, 378, 561, 1093, 1507
Bme1390I	CCNGG	7 cut(s)	246, 272, 279, 388, 389, 688, 1377
Bme18I	GGWCC	4 cut(s)	202, 242, 370, 1373
BmeRI	GACNNNNNGTC	3 cut(s)	31, 34, 368
BmeT110I	CYCGRG	2 cut(s)	387, 1382
BmgT120I	GGNCC	6 cut(s)	202, 242, 348, 370, 450, 1373
BmiI	GGNNCC	4 cut(s)	204, 276, 744, 1549
BmrFI	CCNGG	7 cut(s)	246, 272, 279, 388, 389, 688, 1377
BmsI	GCATC	1 cut(s)	166
BmtI	GCTAGC	1 cut(s)	485
BpiI	GAAGAC	2 cut(s)	27, 1354
BpmI	CTGGAG	2 cut(s)	284, 591
Bpu10I	CCTNAGC	1 cut(s)	1178
BpuEI	CTTGAG	1 cut(s)	635
BpuMI	CCSGG	4 cut(s)	246, 279, 388, 389
BsaBI	GATNNNNATC	2 cut(s)	164, 1421
BsaI	GGTCTC	1 cut(s)	1385
BsaJI	CCNNGG	7 cut(s)	195, 357, 387, 547, 966, 1332, 1341
BsaWI	WCCGGW	1 cut(s)	239
BsaXI	ACNNNNNCTCC	4 cut(s)	125, 155, 470, 500
Bsc4I	CCNNNNNNNGG	3 cut(s)	277, 393, 394
Bse118I	RCCGGY	1 cut(s)	317
Bse1I	ACTGG	3 cut(s)	301, 1011, 1120
Bse3DI	GCAATG	1 cut(s)	525
Bse8I	GATNNNNATC	2 cut(s)	164, 1421
BseBI	CCWGG	3 cut(s)	272, 688, 1377
BseDI	CCNNGG	7 cut(s)	195, 357, 387, 547, 966, 1332, 1341
BseGI	GGATG	2 cut(s)	696, 1267
BseJI	GATNNNNATC	2 cut(s)	164, 1421
BseLI	CCNNNNNNNGG	3 cut(s)	277, 393, 394
BseMI	GCAATG	1 cut(s)	525
BseMII	CTCAG	3 cut(s)	533, 1169, 1239
BseNI	ACTGG	3 cut(s)	301, 1011, 1120
BseRI	GAGGAG	4 cut(s)	146, 449, 1059, 1482
BseSI	GKGCMC	1 cut(s)	1552
BseXI	GCAGC	6 cut(s)	30, 69, 97, 315, 363, 1492
BseYI	CCCAGC	1 cut(s)	1127
Bsh1236I	CGCG	6 cut(s)	56, 151, 311, 401, 1195, 1540
BshFI	GGCC	2 cut(s)	350, 452
BshNI	GGYRCC	1 cut(s)	1547
BsiHKAI	GWGCWC	2 cut(s)	426, 465
BsiHKCI	CYCGRG	2 cut(s)	387, 1382
BsiSI	CCGG	5 cut(s)	240, 245, 278, 318, 388
BslFI	GGGAC	4 cut(s)	79, 134, 188, 375
BslI	CCNNNNNNNGG	3 cut(s)	277, 393, 394
BsmAI	GTCTC	2 cut(s)	42, 1385
BsmBI	CGTCTC	1 cut(s)	42
BsmFI	GGGAC	4 cut(s)	79, 134, 188, 375
BsmI	GAATGC	1 cut(s)	823
BsnI	GGCC	2 cut(s)	350, 452
Bso31I	GGTCTC	1 cut(s)	1385
BsoBI	CYCGRG	2 cut(s)	387, 1382
Bsp1286I	GDGCHC	3 cut(s)	426, 465, 1552
Bsp19I	CCATGG	2 cut(s)	357, 547
Bsp68I	TCGCGA	1 cut(s)	401
BspANI	GGCC	2 cut(s)	350, 452
BspCNI	CTCAG	3 cut(s)	534, 1170, 1240
BspFNI	CGCG	6 cut(s)	56, 151, 311, 401, 1195, 1540
BspLI	GGNNCC	4 cut(s)	204, 276, 744, 1549
BspMAI	CTGCAG	2 cut(s)	87, 1496
BspMI	ACCTGC	2 cut(s)	1007, 1339
BspOI	GCTAGC	1 cut(s)	485
BspPI	GGATC	5 cut(s)	154, 269, 282, 303, 848
BspT107I	GGYRCC	1 cut(s)	1547
BspTNI	GGTCTC	1 cut(s)	1385
BsrBI	CCGCTC	1 cut(s)	562
BsrDI	GCAATG	1 cut(s)	525
BsrFI	RCCGGY	1 cut(s)	317
BsrI	ACTGG	3 cut(s)	301, 1011, 1120
BssAI	RCCGGY	1 cut(s)	317
BssECI	CCNNGG	7 cut(s)	195, 357, 387, 547, 966, 1332, 1341
BssT1I	CCWWGG	5 cut(s)	357, 547, 966, 1332, 1341
Bst2UI	CCWGG	3 cut(s)	272, 688, 1377
Bst4CI	ACNGT	4 cut(s)	840, 958, 1402, 1578
BstC8I	GCNNGC	4 cut(s)	80, 483, 774, 1089
BstDEI	CTNAG	4 cut(s)	542, 752, 1178, 1248
BstDSI	CCRYGG	3 cut(s)	195, 357, 547
BstF5I	GGATG	2 cut(s)	696, 1267
BstFNI	CGCG	6 cut(s)	56, 151, 311, 401, 1195, 1540
BstH2I	RGCGCY	2 cut(s)	9, 525
BstHHI	GCGC	4 cut(s)	8, 151, 311, 524
BstMAI	GTCTC	2 cut(s)	42, 1385
BstMWI	GCNNNNNNNGC	6 cut(s)	88, 146, 334, 373, 482, 1285
BstNI	CCWGG	3 cut(s)	272, 688, 1377
BstSCI	CCNGG	7 cut(s)	244, 270, 277, 386, 387, 686, 1375
BstSFI	CTRYAG	4 cut(s)	83, 427, 1218, 1492
BstSLI	GKGCMC	1 cut(s)	1552
BstUI	CGCG	6 cut(s)	56, 151, 311, 401, 1195, 1540
BstV1I	GCAGC	6 cut(s)	30, 69, 97, 315, 363, 1492
BstV2I	GAAGAC	2 cut(s)	27, 1354
BstX2I	RGATCY	4 cut(s)	159, 274, 295, 1244
BstXI	CCANNNNNNTGG	1 cut(s)	358
BstYI	RGATCY	4 cut(s)	159, 274, 295, 1244
BsuI	GTATCC	1 cut(s)	450
BsuRI	GGCC	2 cut(s)	350, 452
BtgI	CCRYGG	3 cut(s)	195, 357, 547
BtsCI	GGATG	2 cut(s)	696, 1267
BtsI	GCAGTG	1 cut(s)	1501
BtsIMutI	CAGTG	2 cut(s)	1501, 1574
BtuMI	TCGCGA	1 cut(s)	401
BveI	ACCTGC	2 cut(s)	1007, 1339
Cac8I	GCNNGC	4 cut(s)	80, 483, 774, 1089
CaiI	CAGNNNCTG	2 cut(s)	97, 541
CfoI	GCGC	4 cut(s)	8, 151, 311, 524
Cfr10I	RCCGGY	1 cut(s)	317
Cfr13I	GGNCC	6 cut(s)	202, 242, 348, 370, 450, 1373
Cfr9I	CCCGGG	1 cut(s)	387
CpoI	CGGWCCG	1 cut(s)	242
CseI	GACGC	1 cut(s)	62
CsiI	ACCWGGT	1 cut(s)	1375
Csp6I	GTAC	2 cut(s)	836, 981
CspI	CGGWCCG	1 cut(s)	242
CviAII	CATG	6 cut(s)	117, 358, 472, 548, 1354, 1395
CviQI	GTAC	2 cut(s)	836, 981
DdeI	CTNAG	4 cut(s)	542, 752, 1178, 1248
DrdI	GACNNNNNNGTC	1 cut(s)	179
DriI	GACNNNNNGTC	3 cut(s)	31, 34, 368
DseDI	GACNNNNNNGTC	1 cut(s)	179
Eam1105I	GACNNNNNGTC	3 cut(s)	31, 34, 368
EciI	GGCGGA	3 cut(s)	434, 491, 512
Ecl136II	GAGCTC	1 cut(s)	424
Eco130I	CCWWGG	5 cut(s)	357, 547, 966, 1332, 1341
Eco24I	GRGCYC	1 cut(s)	426
Eco31I	GGTCTC	1 cut(s)	1385
Eco47I	GGWCC	4 cut(s)	202, 242, 370, 1373
Eco53kI	GAGCTC	1 cut(s)	424
Eco57I	CTGAAG	3 cut(s)	1013, 1274, 1479
Eco88I	CYCGRG	2 cut(s)	387, 1382
EcoICRI	GAGCTC	1 cut(s)	424
EcoRII	CCWGG	3 cut(s)	270, 686, 1375
EcoT14I	CCWWGG	5 cut(s)	357, 547, 966, 1332, 1341
EcoT38I	GRGCYC	1 cut(s)	426
ErhI	CCWWGG	5 cut(s)	357, 547, 966, 1332, 1341
Esp3I	CGTCTC	1 cut(s)	42
FaeI	CATG	6 cut(s)	120, 361, 475, 551, 1357, 1398
FaqI	GGGAC	4 cut(s)	79, 134, 188, 375
FatI	CATG	6 cut(s)	116, 357, 471, 547, 1353, 1394
FauI	CCCGC	2 cut(s)	144, 213
FbaI	TGATCA	3 cut(s)	936, 1188, 1422
FblI	GTMKAC	3 cut(s)	113, 171, 183
Fnu4HI	GCNGC	9 cut(s)	44, 83, 86, 89, 329, 377, 560, 1092, 1506
FokI	GGATG	2 cut(s)	703, 1274
FriOI	GRGCYC	1 cut(s)	426
Fsp4HI	GCNGC	9 cut(s)	44, 83, 86, 89, 329, 377, 560, 1092, 1506
FspBI	CTAG	1 cut(s)	482
GlaI	GCGC	4 cut(s)	7, 150, 310, 523
GluI	GCNGC	9 cut(s)	44, 83, 86, 89, 329, 377, 560, 1092, 1506
GsaI	CCCAGC	1 cut(s)	1131
GsuI	CTGGAG	2 cut(s)	284, 591
HaeII	RGCGCY	2 cut(s)	9, 525
HaeIII	GGCC	2 cut(s)	350, 452
HapII	CCGG	5 cut(s)	240, 245, 278, 318, 388
HgaI	GACGC	1 cut(s)	62
HhaI	GCGC	4 cut(s)	8, 151, 311, 524
Hin1II	CATG	6 cut(s)	120, 361, 475, 551, 1357, 1398
Hin6I	GCGC	4 cut(s)	6, 149, 309, 522
HinP1I	GCGC	4 cut(s)	6, 149, 309, 522
HincII	GTYRAC	4 cut(s)	114, 172, 184, 700
HindII	GTYRAC	4 cut(s)	114, 172, 184, 700
HinfI	GANTC	8 cut(s)	32, 191, 532, 905, 1060, 1297, 1303, 1595
HpaII	CCGG	5 cut(s)	240, 245, 278, 318, 388
HphI	GGTGA	5 cut(s)	229, 640, 943, 1426, 1432
Hpy166II	GTNNAC	6 cut(s)	114, 172, 184, 237, 700, 1574
Hpy188I	TCNGA	9 cut(s)	444, 537, 579, 858, 1059, 1249, 1302, 1459, 1466
Hpy8I	GTNNAC	6 cut(s)	114, 172, 184, 237, 700, 1574
HpyAV	CCTTC	2 cut(s)	284, 635
HpyCH4III	ACNGT	4 cut(s)	840, 958, 1402, 1578
HpyCH4IV	ACGT	2 cut(s)	813, 1121
HpyCH4V	TGCA	8 cut(s)	85, 653, 823, 917, 1016, 1205, 1494, 1592
HpyF10VI	GCNNNNNNNGC	6 cut(s)	88, 146, 334, 373, 482, 1285
HpyF3I	CTNAG	4 cut(s)	542, 752, 1178, 1248
HpySE526I	ACGT	2 cut(s)	813, 1121
Hsp92II	CATG	6 cut(s)	120, 361, 475, 551, 1357, 1398
HspAI	GCGC	4 cut(s)	6, 149, 309, 522
Ksp22I	TGATCA	3 cut(s)	936, 1188, 1422
LmnI	GCTCC	4 cut(s)	421, 478, 519, 1345
Lsp1109I	GCAGC	6 cut(s)	30, 69, 97, 315, 363, 1492
LweI	GCATC	1 cut(s)	166
MabI	ACCWGGT	1 cut(s)	1375
MaeI	CTAG	1 cut(s)	482
MaeII	ACGT	2 cut(s)	813, 1121
MaeIII	GTNAC	3 cut(s)	320, 949, 1601
MbiI	CCGCTC	1 cut(s)	562
MboII	GAAGA	8 cut(s)	32, 169, 232, 1019, 1151, 1180, 1354, 1441
MfeI	CAATTG	4 cut(s)	11, 900, 912, 1206
MflI	RGATCY	4 cut(s)	159, 274, 295, 1244
MhlI	GDGCHC	3 cut(s)	426, 465, 1552
MluCI	AATT	8 cut(s)	11, 283, 765, 900, 912, 940, 1206, 1230
MlyI	GAGTC	2 cut(s)	41, 914
MmeI	TCCRAC	3 cut(s)	618, 1239, 1422
MseI	TTAA	5 cut(s)	341, 728, 1155, 1239, 1281
MslI	CAYNNNNRTG	1 cut(s)	356
MspA1I	CMGCKG	2 cut(s)	376, 1505
MspI	CCGG	5 cut(s)	240, 245, 278, 318, 388
MspR9I	CCNGG	7 cut(s)	246, 272, 279, 388, 389, 688, 1377
MunI	CAATTG	4 cut(s)	11, 900, 912, 1206
Mva1269I	GAATGC	1 cut(s)	823
MvaI	CCWGG	3 cut(s)	272, 688, 1377
MvnI	CGCG	6 cut(s)	56, 151, 311, 401, 1195, 1540
MwoI	GCNNNNNNNGC	6 cut(s)	88, 146, 334, 373, 482, 1285
NciI	CCSGG	4 cut(s)	246, 279, 388, 389
NcoI	CCATGG	2 cut(s)	357, 547
NheI	GCTAGC	1 cut(s)	481
NlaIII	CATG	6 cut(s)	120, 361, 475, 551, 1357, 1398
NlaIV	GGNNCC	4 cut(s)	204, 276, 744, 1549
NmuCI	GTSAC	1 cut(s)	949
NruI	TCGCGA	1 cut(s)	401
PaeR7I	CTCGAG	1 cut(s)	1382
PcsI	WCGNNNNNNNCGW	4 cut(s)	57, 107, 177, 180
PctI	GAATGC	1 cut(s)	823
PfeI	GAWTC	6 cut(s)	191, 532, 1060, 1297, 1303, 1595
PflFI	GACNNNGTC	1 cut(s)	1377
PfoI	TCCNGGA	1 cut(s)	270
PkrI	GCNGC	9 cut(s)	45, 84, 87, 90, 330, 378, 561, 1093, 1507
PleI	GAGTC	2 cut(s)	40, 913
PpsI	GAGTC	2 cut(s)	40, 913
PsiI	TTATAA	1 cut(s)	1139
Psp124BI	GAGCTC	1 cut(s)	426
Psp6I	CCWGG	3 cut(s)	270, 686, 1375
PspFI	CCCAGC	1 cut(s)	1127
PspGI	CCWGG	3 cut(s)	270, 686, 1375
PspN4I	GGNNCC	4 cut(s)	204, 276, 744, 1549
PspPI	GGNCC	6 cut(s)	202, 242, 348, 370, 450, 1373
PstI	CTGCAG	2 cut(s)	87, 1496
PstNI	CAGNNNCTG	2 cut(s)	97, 541
PsuI	RGATCY	4 cut(s)	159, 274, 295, 1244
PsyI	GACNNNGTC	1 cut(s)	1377
PvuII	CAGCTG	2 cut(s)	376, 1505
RruI	TCGCGA	1 cut(s)	401
RsaI	GTAC	2 cut(s)	837, 982
RsaNI	GTAC	2 cut(s)	836, 981
RseI	CAYNNNNRTG	1 cut(s)	356
Rsr2I	CGGWCCG	1 cut(s)	242
RsrII	CGGWCCG	1 cut(s)	242
SacI	GAGCTC	1 cut(s)	426
SalI	GTCGAC	3 cut(s)	112, 170, 182
SaqAI	TTAA	5 cut(s)	341, 728, 1155, 1239, 1281
SatI	GCNGC	9 cut(s)	44, 83, 86, 89, 329, 377, 560, 1092, 1506
Sau96I	GGNCC	6 cut(s)	202, 242, 348, 370, 450, 1373
SchI	GAGTC	2 cut(s)	41, 914
ScrFI	CCNGG	7 cut(s)	246, 272, 279, 388, 389, 688, 1377
SduI	GDGCHC	3 cut(s)	426, 465, 1552
SexAI	ACCWGGT	1 cut(s)	1375
SfaNI	GCATC	1 cut(s)	166
SfcI	CTRYAG	4 cut(s)	83, 427, 1218, 1492
Sfr274I	CTCGAG	1 cut(s)	1382
SgrDI	CGTCGACG	2 cut(s)	170, 182
SinI	GGWCC	4 cut(s)	202, 242, 370, 1373
SlaI	CTCGAG	1 cut(s)	1382
SmaI	CCCGGG	1 cut(s)	389
SmiMI	CAYNNNNRTG	1 cut(s)	356
SmlI	CTYRAG	2 cut(s)	614, 1382
SmoI	CTYRAG	2 cut(s)	614, 1382
Sse9I	AATT	8 cut(s)	11, 283, 765, 900, 912, 940, 1206, 1230
SspI	AATATT	1 cut(s)	850
SspMI	CTAG	1 cut(s)	482
SstI	GAGCTC	1 cut(s)	426
StyD4I	CCNGG	7 cut(s)	244, 270, 277, 386, 387, 686, 1375
StyI	CCWWGG	5 cut(s)	357, 547, 966, 1332, 1341
TaaI	ACNGT	4 cut(s)	840, 958, 1402, 1578
TaiI	ACGT	2 cut(s)	816, 1124
TaqI	TCGA	8 cut(s)	38, 113, 144, 171, 183, 758, 1295, 1383
TasI	AATT	8 cut(s)	11, 283, 765, 900, 912, 940, 1206, 1230
TauI	GCSGC	3 cut(s)	91, 562, 1094
TfiI	GAWTC	6 cut(s)	191, 532, 1060, 1297, 1303, 1595
Tru1I	TTAA	5 cut(s)	341, 728, 1155, 1239, 1281
Tru9I	TTAA	5 cut(s)	341, 728, 1155, 1239, 1281
TscAI	CASTG	2 cut(s)	1501, 1581
TseFI	GTSAC	1 cut(s)	949
TseI	GCWGC	6 cut(s)	43, 82, 85, 328, 376, 1505
Tsp45I	GTSAC	1 cut(s)	949
TspDTI	ATGAA	4 cut(s)	460, 589, 1287, 1441
TspMI	CCCGGG	1 cut(s)	387
TspRI	CASTG	2 cut(s)	1501, 1581
Tth111I	GACNNNGTC	1 cut(s)	1377
VpaK11BI	GGWCC	4 cut(s)	202, 242, 370, 1373
XcmI	CCANNNNNNNNNTGG	1 cut(s)	364
XhoI	CTCGAG	1 cut(s)	1382
XmaI	CCCGGG	1 cut(s)	387
XmiI	GTMKAC	3 cut(s)	113, 171, 183
XspI	CTAG	1 cut(s)	482

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.