Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0013937

Physical Location & Seq

Genomic Coordinates Reverse (-)

2831291 .. 2833874

Gene Structure 2584 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000010591

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1608 bp

ATGAGCGCCCAATTGCGCCAAGACGACCAGCTCAGTCAACATGCCAAGGACGACGACGAAGAAGAAGCCAGCCGCTTATTGCGTCCGCAATCCTCGTTCTCCGACGTCGACGATGAGGTGGCGTACGAGTCGCGGGAGAAGATCCTGATTGTCGACGCCGATGCCGACTCGGCCGCCGACTACGACGATTCGACTCAGGTCCCGCCGCCCTTCTCCTGGAAGAAGCTCTGGCTGTTCACAGGCCCGGGGTTCCTGATGAGCATAGCGTTTTTGGATCCGGGGAATTTAGAAGGGGATCTCCAGGCGGGAGCGATCGCCGGCTACTCTTTGTTGTGGCTGTTAATGTGGGCCACCGCCATGGGCCTGCTGGTCCAGCTGCTCTCGGCCCGCCTCGGAGTCGCCACCGGCCGGCACCTGGCGGAGCTCTGCCGCGACGAGTATCCCAAGTGGGCCGGGCTGGTGCTCTGGTTCATGGCTGAGCTGGCGTTGATTGGGGCGGATATTCAGGAGGTCATTGGGAGTGCCATTGCTATTCAGATTCTCAGCAATGGCTTTATGCCGCTCTGGGCCGGAGTGCTCATTACGGCGTCGGATTGTTTCCTTCTTTTAATTCTTGAGAACTATGGGGTGAGGAAGTTGGAAGGTGTTTTTGCAGTTCTCATTGCAACTATGGCTTTATCGTTTGCCTGGATGTTTGTTGACACAAAACCAAGTGGAAAAGAACTTTTAATCGGTATTCTGGTTCCAAGACTTAGCTCAAGAACAATTCGGCAGGCTGTGGGAGTTGTGGGTTGCGTCATTATGCCACACAATGTATTTTTGCACTCTGCTTTAGTCCAGTCAAGAAATATTGATCCTCACAAGAAAGGACGGGTTCAAGAGGCACTGAACTACTATTCAATTGAGTCATCATTTGCACTTTTTATCTCCTTCGTCATCAATTTGTTTGTGACCACTGTTTTCGCCAAGGGATTTTATGGTACTAAACAAGCTGATAGTATAGGACTAGTTAATGCAGGTCAGTTTCTTCAGGAGAAGTATGGAGGGGGGCTTTATCCAATTCTTTATATCTGGGGTGTTGGGTTATTGGCAGCTGGACAAAGTAGCACAATAACTGGGACATATGCTGGGCAGTTTATCATGGGAGGCTTCCTTAATCTTCGTTTGAAGAAGTGGCTGAGGGCATTGATCACAAGGAGTTTTGCTATTGTGCCAACTATAATTGTGGCAATTATCTTTAATACATCTGAGGGGTCGCTGGATGTTTTAAATGAGTGGCTTAATGTACTTCAGTCGGTTCAGATTCCTTTTGCTCTTATACCACTTCTCACTTTGGTGTCAAAGGAGCAGATCATGGGGGTCTTCAAGATTGGACCTCTACTCGAAAAAGTGGCATGGACGGTGGCTGTTTTGGTGATGGTAATCAATGGTTATCTATTGCTGGATTTCTTCATTGCTGAAGTTAATGGACTTCTGTTTGGTGTTGTGGTCTGTAGTGGTACGGCTGCATATGTAGCGTTTATTATGTATCTGGTATTACGCAGTGGTGCCCTTCCTTCCACTTATTTCAATTCAGTGTACAAGAGATTTGCTGACACAGATACTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000041 GO:0001558 GO:0003674 GO:0005215 GO:0005384 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005802 GO:0006810 GO:0006811 GO:0006812 GO:0006828 GO:0007275 GO:0008150 GO:0008324 GO:0009653 GO:0009888 GO:0009987 GO:0010015 GO:0010035 GO:0010038 GO:0010042 GO:0010053 GO:0010054 GO:0012505 GO:0015075 GO:0015318 GO:0016049 GO:0021700 GO:0022603 GO:0022604 GO:0022622 GO:0022857 GO:0022890 GO:0030001 GO:0030154 GO:0030307 GO:0031984 GO:0032501 GO:0032502 GO:0034220 GO:0040007 GO:0040008 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045927 GO:0046873 GO:0046915 GO:0048364 GO:0048468 GO:0048469 GO:0048518 GO:0048522 GO:0048588 GO:0048589 GO:0048638 GO:0048639 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051510 GO:0051512 GO:0051716 GO:0055085 GO:0060560 GO:0065007 GO:0070887 GO:0071241 GO:0071248 GO:0071287 GO:0071421 GO:0071695 GO:0080147 GO:0090558 GO:0090627 GO:0098655 GO:0098660 GO:0098662 GO:0098791 GO:0099402 GO:1905392

KEGG Pathways

Metabolic & Signaling

ko04142 ko04216 ko04978 map04142 map04216 map04978

Pfam Domains

Protein Families

Nramp

Protein Analysis

536

Amino Acids

58.7

Weight (kDa)

5.07

Isoelectric Point (pI)

36.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Nramp	PF01566	97 - 458	6.1e-114	Natural resistance-associated macrophage protein-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013937)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G47240 AT4G18790
fragaria_vesca	FvH4_2g33450 FvH4_2g33450
malus_domestica	MD08G1089600.v1.1 MD15G1074200.v1.1
prunus_persica	Prupe.1G427000_v2.0.a1 Prupe.1G427000_v2.0.a1
pyrus_communis	pycom08g07290 pycom15g06940
rosa_chinensis	RchiOBHm_Chr6g0309081
rosa_laevigata	RLG00000010591
rosa_roxburghii	Rroxscaffold_7G00159660
rosa_rugosa	Rorug06G0369600
rosa_samantha	Rh6AG482300 Rh6CG496100
rosa_wichuraiana	Rw6G041990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	108
Acc36I	ACCTGC	1 cut(s)	1007
AccB1I	GGYRCC	2 cut(s)	411, 1547
AccBSI	CCGCTC	1 cut(s)	562
AccI	GTMKAC	2 cut(s)	108, 153
AccII	CGCG	2 cut(s)	133, 432
AclWI	GGATC	5 cut(s)	136, 269, 282, 303, 848
AcoI	YGGCCR	2 cut(s)	171, 406
AcsI	RAATTY	1 cut(s)	283
AcuI	CTGAAG	3 cut(s)	1013, 1274, 1479
AcyI	GRCGYC	3 cut(s)	105, 156, 587
AfaI	GTAC	5 cut(s)	125, 982, 1287, 1501, 1580
AfiI	CCNNNNNNNGG	3 cut(s)	216, 408, 415
AgsI	TTSAA	5 cut(s)	878, 900, 1168, 1366, 1570
AhlI	ACTAGT	1 cut(s)	1006
AjnI	CCWGG	4 cut(s)	215, 300, 414, 686
AleI	CACNNNNGTG	1 cut(s)	1334
AluBI	AGCT	8 cut(s)	31, 226, 376, 424, 481, 756, 992, 1094
AluI	AGCT	8 cut(s)	31, 226, 376, 424, 481, 756, 992, 1094
Alw21I	GWGCWC	3 cut(s)	426, 465, 579
AlwI	GGATC	5 cut(s)	136, 269, 282, 303, 848
Ama87I	CYCGRG	1 cut(s)	244
AoxI	GGCC	8 cut(s)	171, 241, 348, 361, 384, 406, 450, 567
ApeKI	GCWGC	3 cut(s)	376, 1091, 1505
ApoI	RAATTY	1 cut(s)	283
AsiSI	GCGATCGC	1 cut(s)	315
AspLEI	GCGC	2 cut(s)	8, 18
AspS9I	GGNCC	9 cut(s)	199, 242, 348, 361, 370, 385, 450, 567, 1373
AsuC2I	CCSGG	4 cut(s)	245, 246, 279, 454
AsuHPI	GGTGA	2 cut(s)	640, 1426
AvaI	CYCGRG	1 cut(s)	244
AvaII	GGWCC	3 cut(s)	199, 370, 1373
BaeGI	GKGCMC	1 cut(s)	1552
BamHI	GGATCC	1 cut(s)	274
BanI	GGYRCC	2 cut(s)	411, 1547
BanII	GRGCYC	1 cut(s)	426
BbsI	GAAGAC	1 cut(s)	1354
Bbv12I	GWGCWC	3 cut(s)	426, 465, 579
BbvCI	CCTCAGC	1 cut(s)	1178
BbvI	GCAGC	3 cut(s)	363, 1103, 1492
BccI	CCATC	1 cut(s)	1411
BceAI	ACGGC	2 cut(s)	600, 1518
BcgI	CGANNNNNNTGC	2 cut(s)	143, 177
BciT130I	CCWGG	4 cut(s)	217, 302, 416, 688
BciVI	GTATCC	1 cut(s)	450
BclI	TGATCA	1 cut(s)	1188
BcnI	CCSGG	4 cut(s)	245, 246, 279, 454
BcuI	ACTAGT	1 cut(s)	1006
BfaI	CTAG	1 cut(s)	1007
BfmI	CTRYAG	1 cut(s)	1492
BfoI	RGCGCY	1 cut(s)	9
BfuAI	ACCTGC	1 cut(s)	1007
BfuI	GTATCC	1 cut(s)	450
BglI	GCCNNNNNGGC	1 cut(s)	170
BisI	GCNGC	8 cut(s)	73, 174, 206, 377, 430, 560, 1092, 1506
BlpI	GCTNAGC	1 cut(s)	477
BlsI	GCNGC	8 cut(s)	74, 175, 207, 378, 431, 561, 1093, 1507
Bme1390I	CCNGG	8 cut(s)	217, 245, 246, 279, 302, 416, 454, 688
Bme18I	GGWCC	3 cut(s)	199, 370, 1373
BmeT110I	CYCGRG	1 cut(s)	244
BmgT120I	GGNCC	9 cut(s)	199, 242, 348, 361, 370, 385, 450, 567, 1373
BmiI	GGNNCC	6 cut(s)	201, 251, 276, 413, 744, 1549
BmrFI	CCNGG	8 cut(s)	217, 245, 246, 279, 302, 416, 454, 688
BmrI	ACTGGG	1 cut(s)	1125
BmsI	GCATC	1 cut(s)	151
BmuI	ACTGGG	1 cut(s)	1125
BoxI	GACNNNNGTC	1 cut(s)	197
BpiI	GAAGAC	1 cut(s)	1354
BpmI	CTGGAG	1 cut(s)	284
Bpu10I	CCTNAGC	1 cut(s)	1178
Bpu1102I	GCTNAGC	1 cut(s)	477
BpuEI	CTTGAG	2 cut(s)	635, 742
BpuMI	CCSGG	4 cut(s)	245, 246, 279, 454
BsaBI	GATNNNNATC	1 cut(s)	1421
BsaHI	GRCGYC	3 cut(s)	105, 156, 587
BsaJI	CCNNGG	7 cut(s)	45, 244, 245, 278, 357, 391, 966
Bsc4I	CCNNNNNNNGG	3 cut(s)	216, 408, 415
Bse118I	RCCGGY	3 cut(s)	317, 404, 408
Bse1I	ACTGG	2 cut(s)	838, 1120
Bse3DI	GCAATG	4 cut(s)	525, 553, 660, 1452
Bse8I	GATNNNNATC	1 cut(s)	1421
BseBI	CCWGG	4 cut(s)	217, 302, 416, 688
BseDI	CCNNGG	7 cut(s)	45, 244, 245, 278, 357, 391, 966
BseGI	GGATG	2 cut(s)	696, 1267
BseJI	GATNNNNATC	1 cut(s)	1421
BseLI	CCNNNNNNNGG	3 cut(s)	216, 408, 415
BseMI	GCAATG	4 cut(s)	525, 553, 660, 1452
BseMII	CTCAG	6 cut(s)	46, 209, 468, 556, 1169, 1239
BseNI	ACTGG	2 cut(s)	838, 1120
BseSI	GKGCMC	1 cut(s)	1552
BseX3I	CGGCCG	2 cut(s)	171, 406
BseXI	GCAGC	3 cut(s)	363, 1103, 1492
BseYI	CCCAGC	1 cut(s)	1127
Bsh1236I	CGCG	2 cut(s)	133, 432
Bsh1285I	CGRYCG	3 cut(s)	174, 315, 409
BshFI	GGCC	8 cut(s)	173, 243, 350, 363, 386, 408, 452, 569
BshNI	GGYRCC	2 cut(s)	411, 1547
BsiEI	CGRYCG	3 cut(s)	174, 315, 409
BsiHKAI	GWGCWC	3 cut(s)	426, 465, 579
BsiHKCI	CYCGRG	1 cut(s)	244
BsiSI	CCGG	7 cut(s)	245, 278, 318, 405, 409, 453, 570
BsiWI	CGTACG	1 cut(s)	123
BslFI	GGGAC	2 cut(s)	185, 1132
BslI	CCNNNNNNNGG	3 cut(s)	216, 408, 415
BsmFI	GGGAC	2 cut(s)	185, 1132
BsnI	GGCC	8 cut(s)	173, 243, 350, 363, 386, 408, 452, 569
BsoBI	CYCGRG	1 cut(s)	244
Bsp1286I	GDGCHC	4 cut(s)	426, 465, 579, 1552
Bsp1407I	TGTACA	1 cut(s)	1578
Bsp143I	GATC	7 cut(s)	141, 274, 295, 312, 853, 1188, 1350
Bsp1720I	GCTNAGC	1 cut(s)	477
Bsp19I	CCATGG	1 cut(s)	357
BspANI	GGCC	8 cut(s)	173, 243, 350, 363, 386, 408, 452, 569
BspCNI	CTCAG	6 cut(s)	45, 208, 469, 555, 1170, 1240
BspFNI	CGCG	2 cut(s)	133, 432
BspLI	GGNNCC	6 cut(s)	201, 251, 276, 413, 744, 1549
BspMI	ACCTGC	1 cut(s)	1007
BspPI	GGATC	5 cut(s)	136, 269, 282, 303, 848
BspT107I	GGYRCC	2 cut(s)	411, 1547
BsrBI	CCGCTC	1 cut(s)	562
BsrDI	GCAATG	4 cut(s)	525, 553, 660, 1452
BsrFI	RCCGGY	3 cut(s)	317, 404, 408
BsrGI	TGTACA	1 cut(s)	1578
BsrI	ACTGG	2 cut(s)	838, 1120
BssAI	RCCGGY	3 cut(s)	317, 404, 408
BssECI	CCNNGG	7 cut(s)	45, 244, 245, 278, 357, 391, 966
BssMI	GATC	7 cut(s)	141, 274, 295, 312, 853, 1188, 1350
BssNI	GRCGYC	3 cut(s)	105, 156, 587
BssT1I	CCWWGG	3 cut(s)	45, 357, 966
Bst2UI	CCWGG	4 cut(s)	217, 302, 416, 688
Bst4CI	ACNGT	2 cut(s)	958, 1402
BstACI	GRCGYC	3 cut(s)	105, 156, 587
BstAUI	TGTACA	1 cut(s)	1578
BstC8I	GCNNGC	7 cut(s)	70, 319, 365, 388, 410, 483, 774
BstDEI	CTNAG	7 cut(s)	32, 195, 477, 542, 752, 1178, 1248
BstDSI	CCRYGG	1 cut(s)	357
BstF5I	GGATG	2 cut(s)	696, 1267
BstFNI	CGCG	2 cut(s)	133, 432
BstH2I	RGCGCY	1 cut(s)	9
BstHHI	GCGC	2 cut(s)	8, 18
BstKTI	GATC	7 cut(s)	144, 277, 298, 315, 856, 1191, 1353
BstMBI	GATC	7 cut(s)	141, 274, 295, 312, 853, 1188, 1350
BstMCI	CGRYCG	3 cut(s)	174, 315, 409
BstMWI	GCNNNNNNNGC	5 cut(s)	170, 373, 482, 671, 1514
BstNI	CCWGG	4 cut(s)	217, 302, 416, 688
BstNSI	RCATGY	1 cut(s)	44
BstPAI	GACNNNNGTC	1 cut(s)	197
BstSCI	CCNGG	8 cut(s)	215, 243, 244, 277, 300, 414, 452, 686
BstSFI	CTRYAG	1 cut(s)	1492
BstSLI	GKGCMC	1 cut(s)	1552
BstUI	CGCG	2 cut(s)	133, 432
BstV1I	GCAGC	3 cut(s)	363, 1103, 1492
BstV2I	GAAGAC	1 cut(s)	1354
BstX2I	RGATCY	3 cut(s)	141, 274, 295
BstXI	CCANNNNNNTGG	1 cut(s)	358
BstYI	RGATCY	3 cut(s)	141, 274, 295
BstZI	CGGCCG	2 cut(s)	171, 406
BsuI	GTATCC	1 cut(s)	450
BsuRI	GGCC	8 cut(s)	173, 243, 350, 363, 386, 408, 452, 569
BtgI	CCRYGG	1 cut(s)	357
BtsCI	GGATG	2 cut(s)	696, 1267
BtsI	GCAGTG	1 cut(s)	1549
BtsIMutI	CAGTG	4 cut(s)	884, 954, 1549, 1581
BveI	ACCTGC	1 cut(s)	1007
Cac8I	GCNNGC	7 cut(s)	70, 319, 365, 388, 410, 483, 774
CfoI	GCGC	2 cut(s)	8, 18
Cfr10I	RCCGGY	3 cut(s)	317, 404, 408
Cfr13I	GGNCC	9 cut(s)	199, 242, 348, 361, 370, 385, 450, 567, 1373
Cfr9I	CCCGGG	1 cut(s)	244
CseI	GACGC	4 cut(s)	71, 164, 576, 784
Csp6I	GTAC	5 cut(s)	124, 981, 1286, 1500, 1579
CviAII	CATG	6 cut(s)	41, 358, 472, 1141, 1354, 1395
CviQI	GTAC	5 cut(s)	124, 981, 1286, 1500, 1579
DdeI	CTNAG	7 cut(s)	32, 195, 477, 542, 752, 1178, 1248
DpnI	GATC	7 cut(s)	143, 276, 297, 314, 855, 1190, 1352
DpnII	GATC	7 cut(s)	141, 274, 295, 312, 853, 1188, 1350
DraI	TTTAAA	1 cut(s)	1269
EaeI	YGGCCR	2 cut(s)	171, 406
EagI	CGGCCG	2 cut(s)	171, 406
EciI	GGCGGA	2 cut(s)	434, 512
Ecl136II	GAGCTC	1 cut(s)	424
EclXI	CGGCCG	2 cut(s)	171, 406
Eco130I	CCWWGG	3 cut(s)	45, 357, 966
Eco24I	GRGCYC	1 cut(s)	426
Eco47I	GGWCC	3 cut(s)	199, 370, 1373
Eco52I	CGGCCG	2 cut(s)	171, 406
Eco53kI	GAGCTC	1 cut(s)	424
Eco57I	CTGAAG	3 cut(s)	1013, 1274, 1479
Eco88I	CYCGRG	1 cut(s)	244
EcoICRI	GAGCTC	1 cut(s)	424
EcoO109I	RGGNCCY	1 cut(s)	199
EcoRII	CCWGG	4 cut(s)	215, 300, 414, 686
EcoT14I	CCWWGG	3 cut(s)	45, 357, 966
EcoT38I	GRGCYC	1 cut(s)	426
ErhI	CCWWGG	3 cut(s)	45, 357, 966
FaeI	CATG	6 cut(s)	44, 361, 475, 1144, 1357, 1398
FaqI	GGGAC	2 cut(s)	185, 1132
FatI	CATG	6 cut(s)	40, 357, 471, 1140, 1353, 1394
FauI	CCCGC	4 cut(s)	126, 210, 298, 395
FauNDI	CATATG	2 cut(s)	1123, 1510
FbaI	TGATCA	1 cut(s)	1188
FblI	GTMKAC	2 cut(s)	108, 153
Fnu4HI	GCNGC	8 cut(s)	73, 174, 206, 377, 430, 560, 1092, 1506
FokI	GGATG	2 cut(s)	703, 1274
FriOI	GRGCYC	1 cut(s)	426
Fsp4HI	GCNGC	8 cut(s)	73, 174, 206, 377, 430, 560, 1092, 1506
FspBI	CTAG	1 cut(s)	1007
GlaI	GCGC	2 cut(s)	7, 17
GluI	GCNGC	8 cut(s)	73, 174, 206, 377, 430, 560, 1092, 1506
GsaI	CCCAGC	1 cut(s)	1131
GsuI	CTGGAG	1 cut(s)	284
HaeII	RGCGCY	1 cut(s)	9
HaeIII	GGCC	8 cut(s)	173, 243, 350, 363, 386, 408, 452, 569
HapII	CCGG	7 cut(s)	245, 278, 318, 405, 409, 453, 570
HgaI	GACGC	4 cut(s)	71, 164, 576, 784
HhaI	GCGC	2 cut(s)	8, 18
Hin1I	GRCGYC	3 cut(s)	105, 156, 587
Hin1II	CATG	6 cut(s)	44, 361, 475, 1144, 1357, 1398
Hin6I	GCGC	2 cut(s)	6, 16
HinP1I	GCGC	2 cut(s)	6, 16
HincII	GTYRAC	4 cut(s)	38, 109, 154, 700
HindII	GTYRAC	4 cut(s)	38, 109, 154, 700
HinfI	GANTC	8 cut(s)	128, 167, 188, 193, 396, 538, 905, 1303
HpaII	CCGG	7 cut(s)	245, 278, 318, 405, 409, 453, 570
HphI	GGTGA	2 cut(s)	640, 1426
Hpy166II	GTNNAC	6 cut(s)	38, 109, 154, 237, 700, 1579
Hpy188I	TCNGA	6 cut(s)	103, 395, 537, 592, 1249, 1302
Hpy188III	TCNNGA	9 cut(s)	145, 253, 506, 614, 759, 843, 878, 1031, 1366
Hpy8I	GTNNAC	6 cut(s)	38, 109, 154, 237, 700, 1579
Hpy99I	CGWCG	9 cut(s)	56, 59, 107, 110, 113, 158, 188, 437, 592
HpyAV	CCTTC	7 cut(s)	220, 284, 611, 635, 940, 1562, 1566
HpyCH4III	ACNGT	2 cut(s)	958, 1402
HpyCH4IV	ACGT	1 cut(s)	105
HpyCH4V	TGCA	6 cut(s)	653, 665, 823, 917, 1016, 1508
HpyF10VI	GCNNNNNNNGC	5 cut(s)	170, 373, 482, 671, 1514
HpyF3I	CTNAG	7 cut(s)	32, 195, 477, 542, 752, 1178, 1248
HpySE526I	ACGT	1 cut(s)	105
Hsp92I	GRCGYC	3 cut(s)	105, 156, 587
Hsp92II	CATG	6 cut(s)	44, 361, 475, 1144, 1357, 1398
HspAI	GCGC	2 cut(s)	6, 16
KroI	GCCGGC	2 cut(s)	317, 408
KroNI	GCCGGC	2 cut(s)	319, 410
Ksp22I	TGATCA	1 cut(s)	1188
Kzo9I	GATC	7 cut(s)	141, 274, 295, 312, 853, 1188, 1350
LmnI	GCTCC	3 cut(s)	308, 421, 1345
Lsp1109I	GCAGC	3 cut(s)	363, 1103, 1492
LweI	GCATC	1 cut(s)	151
MaeI	CTAG	1 cut(s)	1007
MaeII	ACGT	1 cut(s)	105
MaeIII	GTNAC	1 cut(s)	949
MalI	GATC	7 cut(s)	143, 276, 297, 314, 855, 1190, 1352
MbiI	CCGCTC	1 cut(s)	562
MboI	GATC	7 cut(s)	141, 274, 295, 312, 853, 1188, 1350
MboII	GAAGA	9 cut(s)	71, 74, 151, 232, 1019, 1151, 1180, 1354, 1441
MfeI	CAATTG	2 cut(s)	11, 900
MflI	RGATCY	3 cut(s)	141, 274, 295
MhlI	GDGCHC	4 cut(s)	426, 465, 579, 1552
MlyI	GAGTC	5 cut(s)	137, 161, 187, 405, 914
MmeI	TCCRAC	3 cut(s)	126, 570, 618
MroNI	GCCGGC	2 cut(s)	317, 408
MslI	CAYNNNNRTG	2 cut(s)	356, 1334
MspA1I	CMGCKG	2 cut(s)	376, 1094
MspI	CCGG	7 cut(s)	245, 278, 318, 405, 409, 453, 570
MspR9I	CCNGG	8 cut(s)	217, 245, 246, 279, 302, 416, 454, 688
MunI	CAATTG	2 cut(s)	11, 900
MvaI	CCWGG	4 cut(s)	217, 302, 416, 688
MvnI	CGCG	2 cut(s)	133, 432
MwoI	GCNNNNNNNGC	5 cut(s)	170, 373, 482, 671, 1514
NaeI	GCCGGC	2 cut(s)	319, 410
NciI	CCSGG	4 cut(s)	245, 246, 279, 454
NcoI	CCATGG	1 cut(s)	357
NdeI	CATATG	2 cut(s)	1123, 1510
NdeII	GATC	7 cut(s)	141, 274, 295, 312, 853, 1188, 1350
NgoMIV	GCCGGC	2 cut(s)	317, 408
NlaIII	CATG	6 cut(s)	44, 361, 475, 1144, 1357, 1398
NlaIV	GGNNCC	6 cut(s)	201, 251, 276, 413, 744, 1549
NmeAIII	GCCGAG	2 cut(s)	149, 362
NmuCI	GTSAC	1 cut(s)	949
NspI	RCATGY	1 cut(s)	44
OliI	CACNNNNGTG	1 cut(s)	1334
PcsI	WCGNNNNNNNCGW	1 cut(s)	162
PdiI	GCCGGC	2 cut(s)	319, 410
PfeI	GAWTC	3 cut(s)	188, 538, 1303
Pfl23II	CGTACG	1 cut(s)	123
PfoI	TCCNGGA	1 cut(s)	215
PkrI	GCNGC	8 cut(s)	74, 175, 207, 378, 431, 561, 1093, 1507
Ple19I	CGATCG	1 cut(s)	315
PleI	GAGTC	5 cut(s)	136, 161, 187, 404, 913
PpsI	GAGTC	5 cut(s)	136, 161, 187, 404, 913
PpuMI	RGGWCCY	1 cut(s)	199
PshAI	GACNNNNGTC	1 cut(s)	197
Psp124BI	GAGCTC	1 cut(s)	426
Psp5II	RGGWCCY	1 cut(s)	199
Psp6I	CCWGG	4 cut(s)	215, 300, 414, 686
PspFI	CCCAGC	1 cut(s)	1127
PspGI	CCWGG	4 cut(s)	215, 300, 414, 686
PspLI	CGTACG	1 cut(s)	123
PspN4I	GGNNCC	6 cut(s)	201, 251, 276, 413, 744, 1549
PspPI	GGNCC	9 cut(s)	199, 242, 348, 361, 370, 385, 450, 567, 1373
PspPPI	RGGWCCY	1 cut(s)	199
PsuI	RGATCY	3 cut(s)	141, 274, 295
PvuI	CGATCG	1 cut(s)	315
PvuII	CAGCTG	2 cut(s)	376, 1094
RgaI	GCGATCGC	1 cut(s)	315
RsaI	GTAC	5 cut(s)	125, 982, 1287, 1501, 1580
RsaNI	GTAC	5 cut(s)	124, 981, 1286, 1500, 1579
RseI	CAYNNNNRTG	2 cut(s)	356, 1334
SacI	GAGCTC	1 cut(s)	426
SalI	GTCGAC	2 cut(s)	107, 152
SatI	GCNGC	8 cut(s)	73, 174, 206, 377, 430, 560, 1092, 1506
Sau3AI	GATC	7 cut(s)	141, 274, 295, 312, 853, 1188, 1350
Sau96I	GGNCC	9 cut(s)	199, 242, 348, 361, 370, 385, 450, 567, 1373
SchI	GAGTC	5 cut(s)	137, 161, 187, 405, 914
ScrFI	CCNGG	8 cut(s)	217, 245, 246, 279, 302, 416, 454, 688
SduI	GDGCHC	4 cut(s)	426, 465, 579, 1552
SfaAI	GCGATCGC	1 cut(s)	315
SfaNI	GCATC	1 cut(s)	151
SfcI	CTRYAG	1 cut(s)	1492
SgfI	GCGATCGC	1 cut(s)	315
SgrDI	CGTCGACG	1 cut(s)	107
SinI	GGWCC	3 cut(s)	199, 370, 1373
SmaI	CCCGGG	1 cut(s)	246
SmiMI	CAYNNNNRTG	2 cut(s)	356, 1334
SmlI	CTYRAG	2 cut(s)	614, 757
SmoI	CTYRAG	2 cut(s)	614, 757
SpeI	ACTAGT	1 cut(s)	1006
SspI	AATATT	1 cut(s)	850
SspMI	CTAG	1 cut(s)	1007
SstI	GAGCTC	1 cut(s)	426
StyD4I	CCNGG	8 cut(s)	215, 243, 244, 277, 300, 414, 452, 686
StyI	CCWWGG	3 cut(s)	45, 357, 966
TaaI	ACNGT	2 cut(s)	958, 1402
TaiI	ACGT	1 cut(s)	108
TaqI	TCGA	4 cut(s)	108, 153, 191, 1383
TatI	WGTACW	2 cut(s)	1285, 1578
TauI	GCSGC	5 cut(s)	75, 176, 208, 432, 562
TfiI	GAWTC	3 cut(s)	188, 538, 1303
TscAI	CASTG	4 cut(s)	891, 961, 1549, 1581
TseFI	GTSAC	1 cut(s)	949
TseI	GCWGC	3 cut(s)	376, 1091, 1505
Tsp45I	GTSAC	1 cut(s)	949
TspDTI	ATGAA	2 cut(s)	460, 1441
TspMI	CCCGGG	1 cut(s)	244
TspRI	CASTG	4 cut(s)	891, 961, 1549, 1581
VpaK11BI	GGWCC	3 cut(s)	199, 370, 1373
XapI	RAATTY	1 cut(s)	283
XceI	RCATGY	1 cut(s)	44
XcmI	CCANNNNNNNNNTGG	1 cut(s)	364
XmaI	CCCGGG	1 cut(s)	244
XmiI	GTMKAC	2 cut(s)	108, 153
XspI	CTAG	1 cut(s)	1007
ZraI	GACGTC	1 cut(s)	106

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.