Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0013937

Physical Location & Seq

Genomic Coordinates Reverse (-)

5068745 .. 5071994

Gene Structure 3250 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1074200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1614 bp

ATGAGCGCCCAATTGCGCGAACACGACGACGAGTCGTTGAAGCTGCGAGACGACGTCGACGACGACGAAGCTAACCGCCTTCTGCAGCCGCAGTCGCTCTCGCTGTCATCGTCGTCGACATGGGACGACGGCGAGGAGGAGGTGGCCTTCGAGGCGCGGGAGAAGATCCTGATCGTCGACGACGCCGTCGACGACGATTCCACGGCGGTCCCGCCTTTTTCGTGGAAGAAGCTGTGGCTGTTCACCGGTCCGGGTTTTCTGATGAGCATAGCATTTCTGGATCCGGGCAATTTAGAAGGGGATCTCCAGTCGGGCGCGATTGCCGGTTACTCGCTGCTATGGCTTTTAATGTGGGCCACCGCCATGGGGCTCCTGGTCCAGCTGCTCTCCGCCCGGGTCGGGGTCGCGACGGGTCGGCACTTGGCGGAGCTTTGTAGGGAGGAGTATCCGAAATGGGCTGGGTTCGTGCTCTGGTTCATGGCGGAGCTGGCGTTGATTGGGGCGGATATTCAGGAGGTCATTGGGAGCGCCATTGCGATTCAGATTCTGAGCCATGGGTTCTTGCCGCTATGGGCTGGAGTTCTGATTACGGCGTTGGATTGTTTCATCTTTTTATTTCTTGAGAACTATGGGGTGAGGAAGTTGGAAGCTGTGTTTGCAGTTCTCATAGCAACTATGGGTTTATCGTTTGCCTGGATGTTTGTTGACACAAAACCGAGTGGGAAAGAACTTCTAATGGGTATTGTGGTTCCAAGACTTAGCTCAAAAACAATTCGGCAGGCTGTGGGAGTTGTGGGTTGCGTCATTATGCCACACAACGTATTTTTGCATTCTGCTCTGGTACAGTCAAGAAACATTGATCCGAACAAGAAAGGAAGGGTTCAAGAGGCACTAAACTACTACTCAATTGAGTCATCAATTGCACTTTTGATCTCCTTCATGATCAATTTGTTTGTCACCACTGTTTTTGCAAAGGGATTCTATGGTACGAAACAAGCTGACAGTATAGGACTAGTAAATGCAGGTCAATTTCTTCAGGAGAAGTATGGAGGAGGGCTTTATCCGATTCTTTATATCTGGGGTATTGGGTTATTGGCGGCTGGACAAAGTAGCACAATTACGGGGACGTATGCTGGGCAGTTTATAATGGGAGGATTCCTTAATCTCCGTTTGAAGAAGTGGCTGAGGGCATTGATCACACGGAGTTTTGCAATTGTGCCTACTATAATTGTAGCAATTATTTTTAACAGATCTGAGGCTTCGTTGGATGTTTTGAATGAATGGCTCAATGTCCTTCAGTCGATTCAGATTCCTTTTGCTCTTATTCCACTTCTTACCTTGGTGTCCAAGGAGCAGGTCATGGGGGTCTTTAAGATTGGACCTGTTCTCGAGAAAGTGGCATGGACTGTGGCTGTTTTGGTGATGGTGATCAATGGTTATCTGTTGTTGGATTTCTTCATTTCTGAAGTTAGAGGAGTGCTGTTTGCTCTTGTGGTCTGCAGTGGCACGGCTGCGTATGTAACATTTGTCATATATCTTGTTTCACGGAGTGGTGTTCTTCCTTATACTTTTTTCAGTTCACTGTCCAAGAGGTTGGCAGATTCAGGTAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000041 GO:0001558 GO:0003674 GO:0005215 GO:0005384 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005802 GO:0006810 GO:0006811 GO:0006812 GO:0006828 GO:0007275 GO:0008150 GO:0008324 GO:0009653 GO:0009888 GO:0009987 GO:0010015 GO:0010035 GO:0010038 GO:0010042 GO:0010053 GO:0010054 GO:0012505 GO:0015075 GO:0015318 GO:0016049 GO:0021700 GO:0022603 GO:0022604 GO:0022622 GO:0022857 GO:0022890 GO:0030001 GO:0030154 GO:0030307 GO:0031984 GO:0032501 GO:0032502 GO:0034220 GO:0040007 GO:0040008 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045927 GO:0046873 GO:0046915 GO:0048364 GO:0048468 GO:0048469 GO:0048518 GO:0048522 GO:0048588 GO:0048589 GO:0048638 GO:0048639 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051510 GO:0051512 GO:0051716 GO:0055085 GO:0060560 GO:0065007 GO:0070887 GO:0071241 GO:0071248 GO:0071287 GO:0071421 GO:0071695 GO:0080147 GO:0090558 GO:0090627 GO:0098655 GO:0098660 GO:0098662 GO:0098791 GO:0099402 GO:1905392

KEGG Pathways

Metabolic & Signaling

ko04142 ko04216 ko04978 map04142 map04216 map04978

Pfam Domains

Protein Families

Nramp

Protein Analysis

538

Amino Acids

58.96

Weight (kDa)

5.17

Isoelectric Point (pI)

36.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Nramp	PF01566	99 - 460	1.3e-114	Natural resistance-associated macrophage protein-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0013937)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G47240 AT4G18790
fragaria_vesca	FvH4_2g33450 FvH4_2g33450
malus_domestica	MD08G1089600.v1.1 MD15G1074200.v1.1
prunus_persica	Prupe.1G427000_v2.0.a1 Prupe.1G427000_v2.0.a1
pyrus_communis	pycom08g07290 pycom15g06940
rosa_chinensis	RchiOBHm_Chr6g0309081
rosa_laevigata	RLG00000010591
rosa_roxburghii	Rroxscaffold_7G00159660
rosa_rugosa	Rorug06G0369600
rosa_samantha	Rh6AG482300 Rh6CG496100
rosa_wichuraiana	Rw6G041990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1145
AasI	GACNNNNNNGTC	1 cut(s)	185
AatII	GACGTC	1 cut(s)	57
Acc36I	ACCTGC	2 cut(s)	1013, 1345
AccI	GTMKAC	4 cut(s)	57, 116, 177, 189
AccII	CGCG	4 cut(s)	18, 157, 317, 407
AclWI	GGATC	5 cut(s)	160, 275, 288, 309, 854
AcuI	CTGAAG	3 cut(s)	1019, 1280, 1485
AcyI	GRCGYC	2 cut(s)	54, 183
AdeI	CACNNNGTG	1 cut(s)	1550
AfaI	GTAC	2 cut(s)	843, 988
AfiI	CCNNNNNNNGG	3 cut(s)	366, 399, 400
AgeI	ACCGGT	1 cut(s)	245
AgsI	TTSAA	4 cut(s)	40, 884, 1174, 1276
AhdI	GACNNNNNGTC	1 cut(s)	31
AhlI	ACTAGT	1 cut(s)	1012
AjnI	CCWGG	2 cut(s)	372, 692
Alw21I	GWGCWC	1 cut(s)	471
Alw26I	GTCTC	1 cut(s)	42
AlwI	GGATC	5 cut(s)	160, 275, 288, 309, 854
AlwNI	CAGNNNCTG	1 cut(s)	547
Ama87I	CYCGRG	2 cut(s)	393, 1388
AoxI	GGCC	2 cut(s)	144, 354
ApeKI	GCWGC	5 cut(s)	43, 85, 334, 382, 1511
AsiGI	ACCGGT	1 cut(s)	245
AspLEI	GCGC	5 cut(s)	8, 18, 157, 317, 530
AspS9I	GGNCC	5 cut(s)	208, 248, 354, 376, 1379
AsuC2I	CCSGG	4 cut(s)	252, 285, 394, 395
AsuHPI	GGTGA	5 cut(s)	235, 646, 949, 1432, 1438
AvaI	CYCGRG	2 cut(s)	393, 1388
AvaII	GGWCC	4 cut(s)	208, 248, 376, 1379
BamHI	GGATCC	1 cut(s)	280
BanII	GRGCYC	1 cut(s)	372
Bbv12I	GWGCWC	1 cut(s)	471
BbvCI	CCTCAGC	1 cut(s)	1184
BbvI	GCAGC	5 cut(s)	30, 97, 321, 369, 1498
BccI	CCATC	1 cut(s)	1417
BceAI	ACGGC	5 cut(s)	145, 170, 219, 606, 1524
BciT130I	CCWGG	2 cut(s)	374, 694
BciVI	GTATCC	1 cut(s)	456
BclI	TGATCA	3 cut(s)	942, 1194, 1428
BcnI	CCSGG	4 cut(s)	252, 285, 394, 395
BcoDI	GTCTC	1 cut(s)	42
BcuI	ACTAGT	1 cut(s)	1012
BfaI	CTAG	1 cut(s)	1013
BfmI	CTRYAG	2 cut(s)	83, 1498
BfoI	RGCGCY	2 cut(s)	9, 531
BfuAI	ACCTGC	2 cut(s)	1013, 1345
BfuI	GTATCC	1 cut(s)	456
BglI	GCCNNNNNGGC	1 cut(s)	152
BglII	AGATCT	1 cut(s)	1250
BisI	GCNGC	8 cut(s)	44, 86, 89, 335, 383, 566, 1098, 1512
BlsI	GCNGC	8 cut(s)	45, 87, 90, 336, 384, 567, 1099, 1513
Bme1390I	CCNGG	6 cut(s)	252, 285, 374, 394, 395, 694
Bme18I	GGWCC	4 cut(s)	208, 248, 376, 1379
BmeRI	GACNNNNNGTC	1 cut(s)	31
BmeT110I	CYCGRG	2 cut(s)	393, 1388
BmgT120I	GGNCC	5 cut(s)	208, 248, 354, 376, 1379
BmiI	GGNNCC	4 cut(s)	210, 282, 371, 750
BmrFI	CCNGG	6 cut(s)	252, 285, 374, 394, 395, 694
BpmI	CTGGAG	2 cut(s)	290, 597
Bpu10I	CCTNAGC	1 cut(s)	1184
BpuEI	CTTGAG	1 cut(s)	641
BpuMI	CCSGG	4 cut(s)	252, 285, 394, 395
BsaBI	GATNNNNATC	2 cut(s)	170, 1427
BsaHI	GRCGYC	2 cut(s)	54, 183
BsaJI	CCNNGG	6 cut(s)	201, 363, 393, 553, 1338, 1347
BsaWI	WCCGGW	1 cut(s)	245
BsaXI	ACNNNNNCTCC	2 cut(s)	476, 506
Bsc4I	CCNNNNNNNGG	3 cut(s)	366, 399, 400
Bse118I	RCCGGY	2 cut(s)	245, 323
Bse1I	ACTGG	1 cut(s)	307
Bse3DI	GCAATG	1 cut(s)	531
Bse8I	GATNNNNATC	2 cut(s)	170, 1427
BseBI	CCWGG	2 cut(s)	374, 694
BseDI	CCNNGG	6 cut(s)	201, 363, 393, 553, 1338, 1347
BseGI	GGATG	2 cut(s)	702, 1273
BseJI	GATNNNNATC	2 cut(s)	170, 1427
BseLI	CCNNNNNNNGG	3 cut(s)	366, 399, 400
BseMI	GCAATG	1 cut(s)	531
BseMII	CTCAG	3 cut(s)	539, 1175, 1245
BseNI	ACTGG	1 cut(s)	307
BseRI	GAGGAG	5 cut(s)	149, 152, 455, 1065, 1488
BseXI	GCAGC	5 cut(s)	30, 97, 321, 369, 1498
BseYI	CCCAGC	2 cut(s)	458, 1133
Bsh1236I	CGCG	4 cut(s)	18, 157, 317, 407
BshFI	GGCC	2 cut(s)	146, 356
BshTI	ACCGGT	1 cut(s)	245
BsiHKAI	GWGCWC	1 cut(s)	471
BsiHKCI	CYCGRG	2 cut(s)	393, 1388
BsiSI	CCGG	5 cut(s)	246, 251, 284, 324, 394
BslFI	GGGAC	3 cut(s)	137, 194, 1138
BslI	CCNNNNNNNGG	3 cut(s)	366, 399, 400
BsmAI	GTCTC	1 cut(s)	42
BsmBI	CGTCTC	1 cut(s)	42
BsmFI	GGGAC	3 cut(s)	137, 194, 1138
BsmI	GAATGC	1 cut(s)	829
BsnI	GGCC	2 cut(s)	146, 356
BsoBI	CYCGRG	2 cut(s)	393, 1388
Bsp1286I	GDGCHC	2 cut(s)	372, 471
Bsp19I	CCATGG	2 cut(s)	363, 553
Bsp68I	TCGCGA	1 cut(s)	407
BspANI	GGCC	2 cut(s)	146, 356
BspCNI	CTCAG	3 cut(s)	540, 1176, 1246
BspFNI	CGCG	4 cut(s)	18, 157, 317, 407
BspHI	TCATGA	1 cut(s)	939
BspLI	GGNNCC	4 cut(s)	210, 282, 371, 750
BspMAI	CTGCAG	2 cut(s)	87, 1502
BspMI	ACCTGC	2 cut(s)	1013, 1345
BspPI	GGATC	5 cut(s)	160, 275, 288, 309, 854
BsrDI	GCAATG	1 cut(s)	531
BsrFI	RCCGGY	2 cut(s)	245, 323
BsrI	ACTGG	1 cut(s)	307
BssAI	RCCGGY	2 cut(s)	245, 323
BssECI	CCNNGG	6 cut(s)	201, 363, 393, 553, 1338, 1347
BssNI	GRCGYC	2 cut(s)	54, 183
BssT1I	CCWWGG	4 cut(s)	363, 553, 1338, 1347
Bst2UI	CCWGG	2 cut(s)	374, 694
Bst4CI	ACNGT	5 cut(s)	846, 964, 1004, 1408, 1584
BstACI	GRCGYC	2 cut(s)	54, 183
BstC8I	GCNNGC	2 cut(s)	489, 780
BstDEI	CTNAG	4 cut(s)	548, 758, 1184, 1254
BstDSI	CCRYGG	3 cut(s)	201, 363, 553
BstF5I	GGATG	2 cut(s)	702, 1273
BstFNI	CGCG	4 cut(s)	18, 157, 317, 407
BstH2I	RGCGCY	2 cut(s)	9, 531
BstHHI	GCGC	5 cut(s)	8, 18, 157, 317, 530
BstMAI	GTCTC	1 cut(s)	42
BstMWI	GCNNNNNNNGC	5 cut(s)	94, 152, 340, 488, 656
BstNI	CCWGG	2 cut(s)	374, 694
BstSCI	CCNGG	6 cut(s)	250, 283, 372, 392, 393, 692
BstSFI	CTRYAG	2 cut(s)	83, 1498
BstUI	CGCG	4 cut(s)	18, 157, 317, 407
BstV1I	GCAGC	5 cut(s)	30, 97, 321, 369, 1498
BstX2I	RGATCY	4 cut(s)	165, 280, 301, 1250
BstXI	CCANNNNNNTGG	2 cut(s)	364, 1594
BstYI	RGATCY	4 cut(s)	165, 280, 301, 1250
BsuI	GTATCC	1 cut(s)	456
BsuRI	GGCC	2 cut(s)	146, 356
BtgI	CCRYGG	3 cut(s)	201, 363, 553
BtsCI	GGATG	2 cut(s)	702, 1273
BtsI	GCAGTG	1 cut(s)	1507
BtsIMutI	CAGTG	3 cut(s)	960, 1507, 1580
BtuMI	TCGCGA	1 cut(s)	407
BveI	ACCTGC	2 cut(s)	1013, 1345
Cac8I	GCNNGC	2 cut(s)	489, 780
CaiI	CAGNNNCTG	1 cut(s)	547
CciI	TCATGA	1 cut(s)	939
CfoI	GCGC	5 cut(s)	8, 18, 157, 317, 530
Cfr10I	RCCGGY	2 cut(s)	245, 323
Cfr13I	GGNCC	5 cut(s)	208, 248, 354, 376, 1379
Cfr9I	CCCGGG	1 cut(s)	393
CpoI	CGGWCCG	1 cut(s)	248
CseI	GACGC	2 cut(s)	191, 790
Csp6I	GTAC	2 cut(s)	842, 987
CspAI	ACCGGT	1 cut(s)	245
CspCI	CAANNNNNGTGG	2 cut(s)	949, 984
CspI	CGGWCCG	1 cut(s)	248
CviAII	CATG	7 cut(s)	120, 364, 478, 554, 940, 1360, 1401
CviQI	GTAC	2 cut(s)	842, 987
DdeI	CTNAG	4 cut(s)	548, 758, 1184, 1254
DraIII	CACNNNGTG	1 cut(s)	1550
DrdI	GACNNNNNNGTC	1 cut(s)	185
DriI	GACNNNNNGTC	1 cut(s)	31
DseDI	GACNNNNNNGTC	1 cut(s)	185
Eam1105I	GACNNNNNGTC	1 cut(s)	31
EciI	GGCGGA	4 cut(s)	379, 440, 497, 518
Eco130I	CCWWGG	4 cut(s)	363, 553, 1338, 1347
Eco24I	GRGCYC	1 cut(s)	372
Eco47I	GGWCC	4 cut(s)	208, 248, 376, 1379
Eco57I	CTGAAG	3 cut(s)	1019, 1280, 1485
Eco88I	CYCGRG	2 cut(s)	393, 1388
EcoRII	CCWGG	2 cut(s)	372, 692
EcoT14I	CCWWGG	4 cut(s)	363, 553, 1338, 1347
EcoT38I	GRGCYC	1 cut(s)	372
ErhI	CCWWGG	4 cut(s)	363, 553, 1338, 1347
Esp3I	CGTCTC	1 cut(s)	42
FaeI	CATG	7 cut(s)	123, 367, 481, 557, 943, 1363, 1404
FaqI	GGGAC	3 cut(s)	137, 194, 1138
FatI	CATG	7 cut(s)	119, 363, 477, 553, 939, 1359, 1400
FauI	CCCGC	2 cut(s)	150, 219
FbaI	TGATCA	3 cut(s)	942, 1194, 1428
FblI	GTMKAC	4 cut(s)	57, 116, 177, 189
Fnu4HI	GCNGC	8 cut(s)	44, 86, 89, 335, 383, 566, 1098, 1512
FokI	GGATG	2 cut(s)	709, 1280
FriOI	GRGCYC	1 cut(s)	372
Fsp4HI	GCNGC	8 cut(s)	44, 86, 89, 335, 383, 566, 1098, 1512
FspBI	CTAG	1 cut(s)	1013
GlaI	GCGC	5 cut(s)	7, 17, 156, 316, 529
GluI	GCNGC	8 cut(s)	44, 86, 89, 335, 383, 566, 1098, 1512
GsaI	CCCAGC	2 cut(s)	462, 1137
GsuI	CTGGAG	2 cut(s)	290, 597
HaeII	RGCGCY	2 cut(s)	9, 531
HaeIII	GGCC	2 cut(s)	146, 356
HapII	CCGG	5 cut(s)	246, 251, 284, 324, 394
HgaI	GACGC	2 cut(s)	191, 790
HhaI	GCGC	5 cut(s)	8, 18, 157, 317, 530
Hin1I	GRCGYC	2 cut(s)	54, 183
Hin1II	CATG	7 cut(s)	123, 367, 481, 557, 943, 1363, 1404
Hin6I	GCGC	5 cut(s)	6, 16, 155, 315, 528
HinP1I	GCGC	5 cut(s)	6, 16, 155, 315, 528
HincII	GTYRAC	5 cut(s)	58, 117, 178, 190, 706
HindII	GTYRAC	5 cut(s)	58, 117, 178, 190, 706
HpaII	CCGG	5 cut(s)	246, 251, 284, 324, 394
HphI	GGTGA	5 cut(s)	235, 646, 949, 1432, 1438
Hpy166II	GTNNAC	7 cut(s)	58, 117, 178, 190, 243, 706, 1580
Hpy8I	GTNNAC	7 cut(s)	58, 117, 178, 190, 243, 706, 1580
HpyAV	CCTTC	6 cut(s)	89, 157, 290, 870, 946, 1304
HpyCH4III	ACNGT	5 cut(s)	846, 964, 1004, 1408, 1584
HpyCH4IV	ACGT	3 cut(s)	54, 819, 1127
HpyCH4V	TGCA	8 cut(s)	85, 659, 829, 923, 971, 1022, 1211, 1500
HpyF10VI	GCNNNNNNNGC	5 cut(s)	94, 152, 340, 488, 656
HpyF3I	CTNAG	4 cut(s)	548, 758, 1184, 1254
HpySE526I	ACGT	3 cut(s)	54, 819, 1127
Hsp92I	GRCGYC	2 cut(s)	54, 183
Hsp92II	CATG	7 cut(s)	123, 367, 481, 557, 943, 1363, 1404
HspAI	GCGC	5 cut(s)	6, 16, 155, 315, 528
Ksp22I	TGATCA	3 cut(s)	942, 1194, 1428
LmnI	GCTCC	5 cut(s)	375, 427, 484, 525, 1351
Lsp1109I	GCAGC	5 cut(s)	30, 97, 321, 369, 1498
MaeI	CTAG	1 cut(s)	1013
MaeII	ACGT	3 cut(s)	54, 819, 1127
MaeIII	GTNAC	3 cut(s)	326, 955, 1519
MboII	GAAGA	6 cut(s)	175, 238, 1025, 1186, 1447, 1550
MfeI	CAATTG	4 cut(s)	11, 906, 918, 1212
MflI	RGATCY	4 cut(s)	165, 280, 301, 1250
MhlI	GDGCHC	2 cut(s)	372, 471
MlyI	GAGTC	2 cut(s)	41, 920
MmeI	TCCRAC	4 cut(s)	576, 624, 1245, 1428
MseI	TTAA	4 cut(s)	347, 1161, 1245, 1371
MslI	CAYNNNNRTG	1 cut(s)	362
MspA1I	CMGCKG	1 cut(s)	382
MspI	CCGG	5 cut(s)	246, 251, 284, 324, 394
MspR9I	CCNGG	6 cut(s)	252, 285, 374, 394, 395, 694
MunI	CAATTG	4 cut(s)	11, 906, 918, 1212
Mva1269I	GAATGC	1 cut(s)	829
MvaI	CCWGG	2 cut(s)	374, 694
MvnI	CGCG	4 cut(s)	18, 157, 317, 407
MwoI	GCNNNNNNNGC	5 cut(s)	94, 152, 340, 488, 656
NciI	CCSGG	4 cut(s)	252, 285, 394, 395
NcoI	CCATGG	2 cut(s)	363, 553
NlaIII	CATG	7 cut(s)	123, 367, 481, 557, 943, 1363, 1404
NlaIV	GGNNCC	4 cut(s)	210, 282, 371, 750
NmuCI	GTSAC	1 cut(s)	955
NruI	TCGCGA	1 cut(s)	407
PaeR7I	CTCGAG	1 cut(s)	1388
PagI	TCATGA	1 cut(s)	939
PcsI	WCGNNNNNNNCGW	7 cut(s)	57, 60, 63, 107, 183, 186, 189
PctI	GAATGC	1 cut(s)	829
PfeI	GAWTC	9 cut(s)	197, 538, 544, 978, 1066, 1155, 1303, 1309, 1601
PflFI	GACNNNGTC	2 cut(s)	53, 185
PinAI	ACCGGT	1 cut(s)	245
PkrI	GCNGC	8 cut(s)	45, 87, 90, 336, 384, 567, 1099, 1513
PleI	GAGTC	2 cut(s)	40, 919
PpsI	GAGTC	2 cut(s)	40, 919
PsiI	TTATAA	1 cut(s)	1145
Psp6I	CCWGG	2 cut(s)	372, 692
PspFI	CCCAGC	2 cut(s)	458, 1133
PspGI	CCWGG	2 cut(s)	372, 692
PspN4I	GGNNCC	4 cut(s)	210, 282, 371, 750
PspPI	GGNCC	5 cut(s)	208, 248, 354, 376, 1379
PstI	CTGCAG	2 cut(s)	87, 1502
PstNI	CAGNNNCTG	1 cut(s)	547
PsuI	RGATCY	4 cut(s)	165, 280, 301, 1250
PsyI	GACNNNGTC	2 cut(s)	53, 185
PvuII	CAGCTG	1 cut(s)	382
RruI	TCGCGA	1 cut(s)	407
RsaI	GTAC	2 cut(s)	843, 988
RsaNI	GTAC	2 cut(s)	842, 987
RseI	CAYNNNNRTG	1 cut(s)	362
Rsr2I	CGGWCCG	1 cut(s)	248
RsrII	CGGWCCG	1 cut(s)	248
SalI	GTCGAC	4 cut(s)	56, 115, 176, 188
SaqAI	TTAA	4 cut(s)	347, 1161, 1245, 1371
SatI	GCNGC	8 cut(s)	44, 86, 89, 335, 383, 566, 1098, 1512
Sau96I	GGNCC	5 cut(s)	208, 248, 354, 376, 1379
SchI	GAGTC	2 cut(s)	41, 920
ScrFI	CCNGG	6 cut(s)	252, 285, 374, 394, 395, 694
SduI	GDGCHC	2 cut(s)	372, 471
SfcI	CTRYAG	2 cut(s)	83, 1498
Sfr274I	CTCGAG	1 cut(s)	1388
SgrDI	CGTCGACG	3 cut(s)	56, 176, 188
SinI	GGWCC	4 cut(s)	208, 248, 376, 1379
SlaI	CTCGAG	1 cut(s)	1388
SmaI	CCCGGG	1 cut(s)	395
SmiMI	CAYNNNNRTG	1 cut(s)	362
SmlI	CTYRAG	2 cut(s)	620, 1388
SmoI	CTYRAG	2 cut(s)	620, 1388
SpeI	ACTAGT	1 cut(s)	1012
SspMI	CTAG	1 cut(s)	1013
StyD4I	CCNGG	6 cut(s)	250, 283, 372, 392, 393, 692
StyI	CCWWGG	4 cut(s)	363, 553, 1338, 1347
TaaI	ACNGT	5 cut(s)	846, 964, 1004, 1408, 1584
TaiI	ACGT	3 cut(s)	57, 822, 1130
TaqI	TCGA	7 cut(s)	57, 116, 150, 177, 189, 1301, 1389
TauI	GCSGC	3 cut(s)	91, 568, 1100
TfiI	GAWTC	9 cut(s)	197, 538, 544, 978, 1066, 1155, 1303, 1309, 1601
Tru1I	TTAA	4 cut(s)	347, 1161, 1245, 1371
Tru9I	TTAA	4 cut(s)	347, 1161, 1245, 1371
TscAI	CASTG	3 cut(s)	967, 1507, 1587
TseFI	GTSAC	1 cut(s)	955
TseI	GCWGC	5 cut(s)	43, 85, 334, 382, 1511
Tsp45I	GTSAC	1 cut(s)	955
TspDTI	ATGAA	5 cut(s)	466, 595, 928, 1293, 1447
TspGWI	ACGGA	3 cut(s)	1157, 1216, 1561
TspMI	CCCGGG	1 cut(s)	393
TspRI	CASTG	3 cut(s)	967, 1507, 1587
Tth111I	GACNNNGTC	2 cut(s)	53, 185
VpaK11BI	GGWCC	4 cut(s)	208, 248, 376, 1379
XcmI	CCANNNNNNNNNTGG	1 cut(s)	370
XhoI	CTCGAG	1 cut(s)	1388
XmaI	CCCGGG	1 cut(s)	393
XmiI	GTMKAC	4 cut(s)	57, 116, 177, 189
XspI	CTAG	1 cut(s)	1013
ZraI	GACGTC	1 cut(s)	55

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.