MD08G1111200.v1.1

Auxin-responsive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0012822
Physical Location & Seq
Reverse (-)
9774753 .. 9777030
2278 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1111200.v1.1.491

Sequence Viewer

Length: 888 bp
ATGGATAATTTGTATAGTATGATTTGTGAGAATAATGGAGTTGTAAGGGCAAATAAGAGAGGGTATGTTGCAGCTGAAGACAAAAAGCTGGAGCTGAGGCTGGGTCCTCCTGGAGGAGAAGATCATCAGTCACTTCTTTCCCTTGGTTGCTGCAACAAGAACAATATTTCTCATGAAGCCAAAAGAGTCTGTCATGAAGAGAAAAAAGAAGAGAGAAAGTGGCTGGCAAACAGCTCATCAGATCCTCCAGCAAGTGCTTTTGAGCTTACAAATTCAACAGCAGCTCGTGACTCTGATCAGAAAAGTAAAAGCAGAATTGCACAAGCTCCAGTTGTCGGATGGCCTCCGATCCGTTCATCCAGGAAAAATCTTGCAATCAGATCATCACCATCAAGCTTCGCAAAGCCGGCAGCTGATTCGGAGTCACCAAATGAAACTTTGAAGGAGGGAAACGGAAAATCTGATAGTACTACTTATTCCAAGGACCACATGTTTGTAAAGATCAACATGGAAGGAGTTCCCATTGGAAGAAAAATTAACCTCAAAGCCTATGATAGTTATGAGAAACTCTCTTTTGCCATAGATGAACTCTTCCAAGGTCTTCTTGCAGCTCAAAGAACTTGTTGTGGTGTGGAGAAAGAAGACAAGAAGGGAGAGACCAAATCAATAACTGAATCATTACATGGCAATGGGAAATATACTCTACTTTATGAGGATAATGAAGGAGACAGGATGCTTGTTGGTGATGTCCCATGGAACATGTTTGTATCGACGGCAAAGAGGCTTCAGGTATTGAAGAGCTCACAGCTTTCCACTCTACAGCTTAGTAGCAATCAGCGTGAAAAGACACCACTTGATTCTACAATGGAAGTTGGAATTGGAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
AUX_IAA

Protein Analysis

296

Amino Acids

32.61

Weight (kDa)

8.4

Isoelectric Point (pI)

43.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 30 - 273 3.9e-60 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012822)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25890
fragaria_vesca FvH4_2g35280
malus_domestica MD08G1111200.v1.1 MD15G1090600.v1.1
prunus_persica Prupe.1G445000_v2.0.a1 Prupe.1G445000_v2.0.a1 Prupe.1G445000_v2.0.a1
pyrus_communis pycom08g09230
rosa_chinensis RchiOBHm_Chr6g0306631
rosa_laevigata RLG00000010778
rosa_multiflora Rmu_sc0005201.1_g000009
rosa_roxburghii Rroxscaffold_7G00161590
rosa_rugosa Rorug06G0351200
rosa_samantha Rh6AG463500 Rh6BG437400 Rh6CG477800 Rh6DG464500
rosa_wichuraiana Rw6G040390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 236, 343
AcsI RAATTY 1 cut(s) 271
AcuI CTGAAG 2 cut(s) 96, 770
AfaI GTAC 1 cut(s) 469
AfiI CCNNNNNNNGG 2 cut(s) 113, 335
AflIII ACRYGT 2 cut(s) 489, 759
AgsI TTSAA 3 cut(s) 276, 442, 796
AjnI CCWGG 2 cut(s) 109, 359
AjuI GAANNNNNNNTTGG 1 cut(s) 861
Alw21I GWGCWC 1 cut(s) 803
Alw26I GTCTC 2 cut(s) 650, 720
AlwI GGATC 2 cut(s) 236, 343
AoxI GGCC 1 cut(s) 341
ApeKI GCWGC 5 cut(s) 71, 150, 281, 410, 608
ApoI RAATTY 1 cut(s) 271
ArsI GACNNNNNNTTYG 2 cut(s) 476, 508
Asp700I GAANNNNTTC 1 cut(s) 516
AspS9I GGNCC 2 cut(s) 104, 484
AsuHPI GGTGA 3 cut(s) 378, 417, 755
AvaII GGWCC 2 cut(s) 104, 484
BanII GRGCYC 1 cut(s) 803
BauI CACGAG 1 cut(s) 285
BbsI GAAGAC 3 cut(s) 84, 593, 648
Bbv12I GWGCWC 1 cut(s) 803
BbvCI CCTCAGC 1 cut(s) 95
BbvI GCAGC 5 cut(s) 83, 137, 293, 422, 620
BccI CCATC 2 cut(s) 333, 397
BceAI ACGGC 1 cut(s) 789
BcgI CGANNNNNNTGC 2 cut(s) 399, 433
BciT130I CCWGG 2 cut(s) 111, 361
BclI TGATCA 1 cut(s) 295
BcoDI GTCTC 2 cut(s) 650, 720
BfmI CTRYAG 1 cut(s) 818
BisI GCNGC 5 cut(s) 72, 151, 282, 411, 609
BlsI GCNGC 5 cut(s) 73, 152, 283, 412, 610
BmcAI AGTACT 1 cut(s) 469
Bme1390I CCNGG 2 cut(s) 111, 361
Bme18I GGWCC 2 cut(s) 104, 484
BmgT120I GGNCC 2 cut(s) 104, 484
BmiI GGNNCC 1 cut(s) 105
BmrFI CCNGG 2 cut(s) 111, 361
BmsI GCATC 1 cut(s) 723
BpiI GAAGAC 3 cut(s) 84, 593, 648
BplI GAGNNNNNCTC 2 cut(s) 554, 586
BpmI CTGGAG 4 cut(s) 110, 132, 231, 312
Bpu10I CCTNAGC 1 cut(s) 95
BsaI GGTCTC 1 cut(s) 650
BsaJI CCNNGG 4 cut(s) 142, 480, 595, 752
Bsc4I CCNNNNNNNGG 2 cut(s) 113, 335
Bse118I RCCGGY 1 cut(s) 406
Bse1I ACTGG 1 cut(s) 329
Bse3DI GCAATG 1 cut(s) 694
BseBI CCWGG 2 cut(s) 111, 361
BseDI CCNNGG 4 cut(s) 142, 480, 595, 752
BseGI GGATG 3 cut(s) 344, 356, 738
BseLI CCNNNNNNNGG 2 cut(s) 113, 335
BseMI GCAATG 1 cut(s) 694
BseMII CTCAG 1 cut(s) 86
BseNI ACTGG 1 cut(s) 329
BseRI GAGGAG 1 cut(s) 129
BseXI GCAGC 5 cut(s) 83, 137, 293, 422, 620
BseYI CCCAGC 1 cut(s) 100
BshFI GGCC 1 cut(s) 343
BsiHKAI GWGCWC 1 cut(s) 803
BsiSI CCGG 1 cut(s) 407
BslFI GGGAC 1 cut(s) 734
BslI CCNNNNNNNGG 2 cut(s) 113, 335
BsmAI GTCTC 2 cut(s) 650, 720
BsmFI GGGAC 1 cut(s) 734
BsnI GGCC 1 cut(s) 343
Bso31I GGTCTC 1 cut(s) 650
Bsp1286I GDGCHC 1 cut(s) 803
Bsp143I GATC 6 cut(s) 121, 241, 295, 348, 380, 501
Bsp19I CCATGG 1 cut(s) 752
BspANI GGCC 1 cut(s) 343
BspCNI CTCAG 1 cut(s) 87
BspHI TCATGA 2 cut(s) 172, 193
BspLI GGNNCC 1 cut(s) 105
BspPI GGATC 2 cut(s) 236, 343
BspQI GCTCTTC 1 cut(s) 791
BspTNI GGTCTC 1 cut(s) 650
BsrDI GCAATG 1 cut(s) 694
BsrFI RCCGGY 1 cut(s) 406
BsrI ACTGG 1 cut(s) 329
BssAI RCCGGY 1 cut(s) 406
BssECI CCNNGG 4 cut(s) 142, 480, 595, 752
BssMI GATC 6 cut(s) 121, 241, 295, 348, 380, 501
BssSI CACGAG 1 cut(s) 285
BssT1I CCWWGG 4 cut(s) 142, 480, 595, 752
Bst2BI CACGAG 1 cut(s) 285
Bst2UI CCWGG 2 cut(s) 111, 361
Bst6I CTCTTC 4 cut(s) 192, 204, 596, 791
BstC8I GCNNGC 2 cut(s) 225, 408
BstDEI CTNAG 2 cut(s) 95, 824
BstDSI CCRYGG 1 cut(s) 752
BstENI CCTNNNNNAGG 1 cut(s) 111
BstF5I GGATG 3 cut(s) 344, 356, 738
BstKTI GATC 6 cut(s) 124, 244, 298, 351, 383, 504
BstMAI GTCTC 2 cut(s) 650, 720
BstMBI GATC 6 cut(s) 121, 241, 295, 348, 380, 501
BstMWI GCNNNNNNNGC 1 cut(s) 407
BstNI CCWGG 2 cut(s) 111, 361
BstNSI RCATGY 2 cut(s) 493, 763
BstSCI CCNGG 2 cut(s) 109, 359
BstSFI CTRYAG 1 cut(s) 818
BstV1I GCAGC 5 cut(s) 83, 137, 293, 422, 620
BstV2I GAAGAC 3 cut(s) 84, 593, 648
BstX2I RGATCY 1 cut(s) 241
BstYI RGATCY 1 cut(s) 241
BsuRI GGCC 1 cut(s) 343
BtgI CCRYGG 1 cut(s) 752
BtsCI GGATG 3 cut(s) 344, 356, 738
Cac8I GCNNGC 2 cut(s) 225, 408
CciI TCATGA 2 cut(s) 172, 193
Cfr10I RCCGGY 1 cut(s) 406
Cfr13I GGNCC 2 cut(s) 104, 484
Csp6I GTAC 1 cut(s) 468
CviAII CATG 7 cut(s) 173, 194, 490, 508, 683, 753, 760
CviQI GTAC 1 cut(s) 468
DdeI CTNAG 2 cut(s) 95, 824
DpnI GATC 6 cut(s) 123, 243, 297, 350, 382, 503
DpnII GATC 6 cut(s) 121, 241, 295, 348, 380, 501
Eam1104I CTCTTC 4 cut(s) 192, 204, 596, 791
EarI CTCTTC 4 cut(s) 192, 204, 596, 791
Ecl136II GAGCTC 1 cut(s) 801
Eco130I CCWWGG 4 cut(s) 142, 480, 595, 752
Eco24I GRGCYC 1 cut(s) 803
Eco31I GGTCTC 1 cut(s) 650
Eco47I GGWCC 2 cut(s) 104, 484
Eco53kI GAGCTC 1 cut(s) 801
Eco57I CTGAAG 2 cut(s) 96, 770
EcoICRI GAGCTC 1 cut(s) 801
EcoNI CCTNNNNNAGG 1 cut(s) 111
EcoO109I RGGNCCY 1 cut(s) 104
EcoRII CCWGG 2 cut(s) 109, 359
EcoT14I CCWWGG 4 cut(s) 142, 480, 595, 752
EcoT38I GRGCYC 1 cut(s) 803
ErhI CCWWGG 4 cut(s) 142, 480, 595, 752
FaeI CATG 7 cut(s) 176, 197, 493, 511, 686, 756, 763
FalI AAGNNNNNCTT 2 cut(s) 588, 620
FaqI GGGAC 1 cut(s) 734
FatI CATG 7 cut(s) 172, 193, 489, 507, 682, 752, 759
FbaI TGATCA 1 cut(s) 295
Fnu4HI GCNGC 5 cut(s) 72, 151, 282, 411, 609
FokI GGATG 3 cut(s) 343, 351, 745
FriOI GRGCYC 1 cut(s) 803
Fsp4HI GCNGC 5 cut(s) 72, 151, 282, 411, 609
GluI GCNGC 5 cut(s) 72, 151, 282, 411, 609
GsaI CCCAGC 1 cut(s) 104
GsuI CTGGAG 4 cut(s) 110, 132, 231, 312
HaeIII GGCC 1 cut(s) 343
HapII CCGG 1 cut(s) 407
Hin1II CATG 7 cut(s) 176, 197, 493, 511, 686, 756, 763
HindIII AAGCTT 1 cut(s) 394
HinfI GANTC 6 cut(s) 186, 290, 416, 422, 674, 857
HpaII CCGG 1 cut(s) 407
HphI GGTGA 3 cut(s) 378, 417, 755
Hpy188I TCNGA 8 cut(s) 241, 295, 300, 338, 348, 380, 421, 463
Hpy188III TCNNGA 3 cut(s) 173, 194, 287
Hpy99I CGWCG 1 cut(s) 775
HpyAV CCTTC 4 cut(s) 436, 506, 643, 716
HpyCH4V TGCA 5 cut(s) 71, 153, 320, 374, 608
HpyF10VI GCNNNNNNNGC 1 cut(s) 407
HpyF3I CTNAG 2 cut(s) 95, 824
Hsp92II CATG 7 cut(s) 176, 197, 493, 511, 686, 756, 763
KroI GCCGGC 1 cut(s) 406
KroNI GCCGGC 1 cut(s) 408
Ksp22I TGATCA 1 cut(s) 295
Kzo9I GATC 6 cut(s) 121, 241, 295, 348, 380, 501
LguI GCTCTTC 1 cut(s) 791
LmnI GCTCC 2 cut(s) 91, 331
Lsp1109I GCAGC 5 cut(s) 83, 137, 293, 422, 620
LweI GCATC 1 cut(s) 723
MaeIII GTNAC 3 cut(s) 129, 287, 423
MalI GATC 6 cut(s) 123, 243, 297, 350, 382, 503
MboI GATC 6 cut(s) 121, 241, 295, 348, 380, 501
MboII GAAGA 9 cut(s) 89, 131, 209, 221, 540, 583, 593, 653, 808
MflI RGATCY 1 cut(s) 241
MhlI GDGCHC 1 cut(s) 803
MluCI AATT 5 cut(s) 7, 271, 315, 534, 876
MlyI GAGTC 3 cut(s) 195, 284, 431
MmeI TCCRAC 2 cut(s) 316, 853
MroNI GCCGGC 1 cut(s) 406
MroXI GAANNNNTTC 1 cut(s) 516
MseI TTAA 1 cut(s) 537
MslI CAYNNNNRTG 1 cut(s) 687
MspA1I CMGCKG 2 cut(s) 74, 413
MspI CCGG 1 cut(s) 407
MspR9I CCNGG 2 cut(s) 111, 361
MvaI CCWGG 2 cut(s) 111, 361
MwoI GCNNNNNNNGC 1 cut(s) 407
NaeI GCCGGC 1 cut(s) 408
NcoI CCATGG 1 cut(s) 752
NdeII GATC 6 cut(s) 121, 241, 295, 348, 380, 501
NgoMIV GCCGGC 1 cut(s) 406
NlaIII CATG 7 cut(s) 176, 197, 493, 511, 686, 756, 763
NlaIV GGNNCC 1 cut(s) 105
NmuCI GTSAC 3 cut(s) 129, 287, 423
NspI RCATGY 2 cut(s) 493, 763
PagI TCATGA 2 cut(s) 172, 193
PciI ACATGT 2 cut(s) 489, 759
PciSI GCTCTTC 1 cut(s) 791
PdiI GCCGGC 1 cut(s) 408
PdmI GAANNNNTTC 1 cut(s) 516
PfeI GAWTC 3 cut(s) 416, 674, 857
PfoI TCCNGGA 2 cut(s) 109, 359
PkrI GCNGC 5 cut(s) 73, 152, 283, 412, 610
PleI GAGTC 3 cut(s) 194, 284, 430
PpsI GAGTC 3 cut(s) 194, 284, 430
PpuMI RGGWCCY 1 cut(s) 104
PscI ACATGT 2 cut(s) 489, 759
Psp124BI GAGCTC 1 cut(s) 803
Psp5II RGGWCCY 1 cut(s) 104
Psp6I CCWGG 2 cut(s) 109, 359
PspFI CCCAGC 1 cut(s) 100
PspGI CCWGG 2 cut(s) 109, 359
PspN4I GGNNCC 1 cut(s) 105
PspPI GGNCC 2 cut(s) 104, 484
PspPPI RGGWCCY 1 cut(s) 104
PsuI RGATCY 1 cut(s) 241
PvuII CAGCTG 2 cut(s) 74, 413
RsaI GTAC 1 cut(s) 469
RsaNI GTAC 1 cut(s) 468
RseI CAYNNNNRTG 1 cut(s) 687
SacI GAGCTC 1 cut(s) 803
SapI GCTCTTC 1 cut(s) 791
SaqAI TTAA 1 cut(s) 537
SatI GCNGC 5 cut(s) 72, 151, 282, 411, 609
Sau3AI GATC 6 cut(s) 121, 241, 295, 348, 380, 501
Sau96I GGNCC 2 cut(s) 104, 484
ScaI AGTACT 1 cut(s) 469
SchI GAGTC 3 cut(s) 195, 284, 431
ScrFI CCNGG 2 cut(s) 111, 361
SduI GDGCHC 1 cut(s) 803
SfaNI GCATC 1 cut(s) 723
SfcI CTRYAG 1 cut(s) 818
SinI GGWCC 2 cut(s) 104, 484
SmiMI CAYNNNNRTG 1 cut(s) 687
Sse9I AATT 5 cut(s) 7, 271, 315, 534, 876
SspI AATATT 1 cut(s) 166
SstI GAGCTC 1 cut(s) 803
StyD4I CCNGG 2 cut(s) 109, 359
StyI CCWWGG 4 cut(s) 142, 480, 595, 752
TaqI TCGA 1 cut(s) 770
TasI AATT 5 cut(s) 7, 271, 315, 534, 876
TatI WGTACW 1 cut(s) 467
TfiI GAWTC 3 cut(s) 416, 674, 857
Tru1I TTAA 1 cut(s) 537
Tru9I TTAA 1 cut(s) 537
TseFI GTSAC 3 cut(s) 129, 287, 423
TseI GCWGC 5 cut(s) 71, 150, 281, 410, 608
Tsp45I GTSAC 3 cut(s) 129, 287, 423
TspDTI ATGAA 6 cut(s) 189, 210, 345, 447, 600, 735
TspGWI ACGGA 2 cut(s) 341, 468
VpaK11BI GGWCC 2 cut(s) 104, 484
XagI CCTNNNNNAGG 1 cut(s) 111
XapI RAATTY 1 cut(s) 271
XceI RCATGY 2 cut(s) 493, 763
XcmI CCANNNNNNNNNTGG 1 cut(s) 336
XmnI GAANNNNTTC 1 cut(s) 516
ZrmI AGTACT 1 cut(s) 469
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.