Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0007010

Physical Location & Seq

Genomic Coordinates Reverse (-)

9814955 .. 9819679

Gene Structure 4725 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1111500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1965 bp

ATGCTGAACATTTCAGCAGGGAAGTCTTGTTCAAACCGAAATCCCACCTTTTATTCCCCTATAAAACGTGGGAGGTTTCTTGTTTTGCAAAACACAAAAAAGTTTGCAGCTTTTGCCTGTTTTCGTTTCTGGAGGAGAATAGTGGTGGGTTTTGGATTTGGAGATCGAAAAAAGGTCGGAGCTTTGATGGGGAGCGTAGTGGAGGAGATAAGCAACGGTGGTGGCCACTCAGTGGTGGATGTTGAAAGTAAGGCTGGCTTTGGTGGAGGTATTGTGGACGTGTACGGTGAGGATTGTGCCACCGAGGATCAGGTTCTCACACCATGGACTGCCTCTGTTGCCAGTGGTTACACATTGCTGCGTGATCCACACTACAACAAAGGTCTTGCCTTCACCGAGAAGGAGAGGGATGCACATTACTTGCGCGGCCTTCTGCCTCCAACCGTCCTAACCCAGGAGCTTCAGAAGAAGTTGATGCAGAATCTTCGCCAATATGAAGTTCCGTTGCATAGGTACATTGCCATGATGGATCTTCAGGAGAGAAATGAAGGGCTGTTTTATAAGCTTTTGATTGACAATGTTGAGGAACTGCTTCCTGTTGTGTACACGCCAACAGTTGGTGAGGCTTGCCAAAAATATGGGAGCATTTTCAGGCATCCTCAGGGTCTTTACATCAGTTTGAAAGAGAAGGGAAAGATCCTTGAAGTACTAAAGAACTGGCCGGACAGGGGTATTCAAGTTATTGTCGTGACTGATGGGGAGCGTATTTTGGGGCTAGGTGATCTTGGCTGCCAGGGCATGGGGATTCCTGTTGGAAAGCTCTCTTTGTATACTGCACTCGGAGGAGTTCCTCCTTCAGCGTGCTTGCCTATAACCATCGATGTTGGAACAAACAACGAGAAGTTGCTGAATGATGAGTTTTACATTGGGATTAAGCAAAGGAGAGCAACTGGACAGGAATATGCGGAGCTTCTCGAAGAATTCATGACTGCAGTTAAGCAGAATTATGGGGAGAAAGTCCTAGTTCAGTTCGAAGATTTTGCAAACCACAATGCATTTGAACTGCTGGCTAAATACAGCAAGACTCACCTTGTCTTCAATGATGACATTCAGGGAACGGCATCTGTGGTCTTAGCAGGGCTCATAGCTTCCTTGAAATTACTTGGTGGAACATTAGCTGACCATACTTTCTTATTTCTGGGTGCTGGAGAGGCTGGAACCGGAATAGCAGAGCTTATAGCTCTTGAGATATCAAAAAAGACCGGCGCTCCATTGGAAAAAGCTCGCAAGAAGATTTGGCTTGTGGATTCTAAGGGACTGATTGTTAAATCTCGTCTAGGATCACTTCAACACTTTAAGCAGCCCTGGGCTCATGATCATGAACCAATAAAGGAACTTGTAGATGCCGTGAAGGCAATCAAGCCAACAGTGCTGATAGGAACATCTGGTGTGGGAAAACAGTTCACGAAGGAGGTCGTTGAGACCATGGCGTCCTTGAATAAGAAACCACTTATCCTTGCTCTTTCCAACCCAACATCGCAAGCTGAGTGTACTGCTGAAGAAGCTTACGCGTGGACAAAGGGTCGAGCAATCTTTGGCAGTGGAAGTCCATTTGATCCTGTCAAATACGAGAACAAACTCCTAGTGCCTGGCCAGGCCAACAACGCGTACATATTCCCCGGTTTTGGCCTGGGATTGATCATGGCTGGTGCCATTCGTGTACACGATGACATGCTTTTGGCAGCCTCTGAAGCTCTGGCTACGCAAGTTTCCGAGGAACACTACGCCAAAGGAATGATCTACCCGCCATTTACCAACATCAGAAAGATATCAGCCAACATTGCAGCTAAGGTCGCTGCTAAGGTCTATGAACTCGGTCTGGCTTCTAATCTTCCTCGACCAAAAGATCTCGTGAAGTATGCAGAGAGCTGCATGTACAGCCCGCGCTACAGAAGTTACCGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004470 GO:0004473 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0005911 GO:0006081 GO:0006082 GO:0006090 GO:0006098 GO:0006108 GO:0006139 GO:0006725 GO:0006732 GO:0006733 GO:0006739 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009051 GO:0009117 GO:0009506 GO:0009507 GO:0009536 GO:0009987 GO:0010035 GO:0010038 GO:0016020 GO:0016043 GO:0016491 GO:0016614 GO:0016615 GO:0016616 GO:0019362 GO:0019637 GO:0019682 GO:0019693 GO:0019752 GO:0022607 GO:0030054 GO:0030312 GO:0031090 GO:0032787 GO:0034641 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043648 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044281 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046496 GO:0046686 GO:0048046 GO:0050896 GO:0051156 GO:0051186 GO:0051259 GO:0051260 GO:0055044 GO:0055086 GO:0055114 GO:0065003 GO:0071704 GO:0071840 GO:0071944 GO:0072524 GO:0098588 GO:0098805 GO:1901135 GO:1901360 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00620 ko00710 ko01100 ko01120 ko01200 map00620 map00710 map01100 map01120 map01200

Pfam Domains

Protein Families

Malic_M malic

Protein Analysis

655

Amino Acids

71.88

Weight (kDa)

8.76

Isoelectric Point (pI)

34.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
malic	PF00390	179 - 359	4.5e-77	Malic enzyme, N-terminal domain
Malic_M	PF03949	370 - 622	1.9e-95	Malic enzyme, NAD binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0007010)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G11670 AT5G25880 AT5G25880 AT5G25880
fragaria_vesca	FvH4_2g35330 FvH4_2g35330 FvH4_2g35330
malus_domestica	MD08G1111500.v1.1 MD15G1091000.v1.1
prunus_persica	Prupe.1G445400_v2.0.a1 Prupe.1G445400_v2.0.a1 Prupe.1G445400_v2.0.a1 Prupe.1G445400_v2.0.a1 Prupe.1G445400_v2.0.a1
pyrus_communis	pycom08g09260 pycom15g08500
rosa_chinensis	RchiOBHm_Chr6g0306591
rosa_laevigata	RLG00000010782
rosa_multiflora	Rmu_sc0017624.1_g000001
rosa_roxburghii	Rroxscaffold_7G00161630
rosa_rugosa	Rorug06G0350700 Rorug06G0350800
rosa_samantha	Rh6AG463100 Rh6BG437800
rosa_wichuraiana	Rw6G040350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	561
AasI	GACNNNNNNGTC	1 cut(s)	1489
AccB1I	GGYRCC	1 cut(s)	1709
AccB7I	CCANNNNNTGG	3 cut(s)	232, 617, 799
AccI	GTMKAC	1 cut(s)	830
AccII	CGCG	4 cut(s)	426, 1571, 1667, 1945
AciI	CCGC	4 cut(s)	426, 965, 1805, 1943
AclWI	GGATC	6 cut(s)	315, 359, 537, 691, 1348, 1610
AcoI	YGGCCR	3 cut(s)	223, 719, 1651
AcsI	RAATTY	1 cut(s)	980
AcuI	CTGAAG	5 cut(s)	446, 518, 840, 1578, 1770
AcyI	GRCGYC	1 cut(s)	1490
AdeI	CACNNNGTG	1 cut(s)	232
AfaI	GTAC	8 cut(s)	284, 515, 605, 708, 1552, 1670, 1722, 1937
AfiI	CCNNNNNNNGG	6 cut(s)	232, 454, 617, 728, 799, 1685
AflIII	ACRYGT	3 cut(s)	279, 1569, 1665
AhdI	GACNNNNNGTC	1 cut(s)	1581
AjiI	CACGTC	1 cut(s)	280
AjnI	CCWGG	6 cut(s)	453, 792, 1364, 1648, 1653, 1689
AjuI	GAANNNNNNNTTGG	2 cut(s)	483, 515
AloI	GAACNNNNNNTCC	2 cut(s)	13, 45
Alw26I	GTCTC	1 cut(s)	1475
AlwI	GGATC	6 cut(s)	315, 359, 537, 691, 1348, 1610
AlwNI	CAGNNNCTG	1 cut(s)	1748
AoxI	GGCC	6 cut(s)	223, 427, 719, 1651, 1656, 1687
ApeKI	GCWGC	8 cut(s)	107, 358, 789, 1360, 1742, 1844, 1856, 1929
ApoI	RAATTY	1 cut(s)	980
Asp700I	GAANNNNTTC	1 cut(s)	591
AspLEI	GCGC	3 cut(s)	426, 1268, 1947
AsuC2I	CCSGG	1 cut(s)	1680
AsuHPI	GGTGA	5 cut(s)	299, 385, 632, 791, 1079
AsuII	TTCGAA	1 cut(s)	1032
AxyI	CCTNAGG	1 cut(s)	660
BalI	TGGCCA	2 cut(s)	225, 1653
BanI	GGYRCC	1 cut(s)	1709
BanII	GRGCYC	2 cut(s)	1143, 1372
BauI	CACGAG	1 cut(s)	1910
BbsI	GAAGAC	1 cut(s)	1087
BbvI	GCAGC	8 cut(s)	119, 345, 776, 1372, 1754, 1843, 1856, 1916
BccI	CCATC	4 cut(s)	181, 520, 749, 884
BceAI	ACGGC	2 cut(s)	1134, 1391
BcgI	CGANNNNNNTGC	4 cut(s)	467, 501, 1022, 1056
BciT130I	CCWGG	6 cut(s)	455, 794, 1366, 1650, 1655, 1691
BclI	TGATCA	2 cut(s)	1375, 1698
BcnI	CCSGG	1 cut(s)	1680
BcoDI	GTCTC	1 cut(s)	1475
BfaI	CTAG	4 cut(s)	776, 1022, 1337, 1643
BfmI	CTRYAG	2 cut(s)	990, 1948
BfoI	RGCGCY	1 cut(s)	1269
BglI	GCCNNNNNGGC	1 cut(s)	1412
BglII	AGATCT	1 cut(s)	1906
BisI	GCNGC	9 cut(s)	108, 359, 427, 790, 1361, 1743, 1845, 1857, 1930
BlsI	GCNGC	9 cut(s)	109, 360, 428, 791, 1362, 1744, 1846, 1858, 1931
BmcAI	AGTACT	1 cut(s)	708
Bme1390I	CCNGG	7 cut(s)	455, 794, 1366, 1650, 1655, 1680, 1691
BmeRI	GACNNNNNGTC	1 cut(s)	1581
BmgBI	CACGTC	1 cut(s)	280
BmiI	GGNNCC	2 cut(s)	1219, 1711
BmrFI	CCNGG	7 cut(s)	455, 794, 1366, 1650, 1655, 1680, 1691
BmsI	GCATC	5 cut(s)	400, 465, 664, 1130, 1393
BpiI	GAAGAC	1 cut(s)	1087
BpmI	CTGGAG	2 cut(s)	151, 1227
Bpu10I	CCTNAGC	2 cut(s)	1848, 1860
Bpu14I	TTCGAA	1 cut(s)	1032
BpuEI	CTTGAG	1 cut(s)	1265
BpuMI	CCSGG	1 cut(s)	1680
Bsa29I	ATCGAT	1 cut(s)	879
BsaHI	GRCGYC	1 cut(s)	1490
BsaI	GGTCTC	1 cut(s)	1475
BsaWI	WCCGGW	1 cut(s)	1220
BsaXI	ACNNNNNCTCC	4 cut(s)	258, 288, 1252, 1282
Bsc4I	CCNNNNNNNGG	6 cut(s)	232, 454, 617, 728, 799, 1685
Bse118I	RCCGGY	1 cut(s)	1262
Bse1I	ACTGG	3 cut(s)	342, 722, 955
Bse21I	CCTNAGG	1 cut(s)	660
Bse3DI	GCAATG	3 cut(s)	353, 516, 1839
BseBI	CCWGG	6 cut(s)	455, 794, 1366, 1650, 1655, 1691
BseCI	ATCGAT	1 cut(s)	879
BseGI	GGATG	3 cut(s)	244, 415, 655
BseLI	CCNNNNNNNGG	6 cut(s)	232, 454, 617, 728, 799, 1685
BseMI	GCAATG	3 cut(s)	353, 516, 1839
BseMII	CTCAG	3 cut(s)	243, 674, 1536
BseNI	ACTGG	3 cut(s)	342, 722, 955
BseRI	GAGGAG	3 cut(s)	148, 218, 858
BseXI	GCAGC	8 cut(s)	119, 345, 776, 1372, 1754, 1843, 1856, 1916
BsgI	GTGCAG	1 cut(s)	819
Bsh1236I	CGCG	4 cut(s)	426, 1571, 1667, 1945
BshFI	GGCC	6 cut(s)	225, 429, 721, 1653, 1658, 1689
BshNI	GGYRCC	1 cut(s)	1709
BshVI	ATCGAT	1 cut(s)	879
BsiSI	CCGG	4 cut(s)	722, 1221, 1263, 1680
BslFI	GGGAC	1 cut(s)	1329
BslI	CCNNNNNNNGG	6 cut(s)	232, 454, 617, 728, 799, 1685
BsmAI	GTCTC	1 cut(s)	1475
BsmFI	GGGAC	1 cut(s)	1329
BsnI	GGCC	6 cut(s)	225, 429, 721, 1653, 1658, 1689
Bso31I	GGTCTC	1 cut(s)	1475
Bsp119I	TTCGAA	1 cut(s)	1032
Bsp1286I	GDGCHC	2 cut(s)	1143, 1372
Bsp1407I	TGTACA	3 cut(s)	603, 1720, 1935
Bsp19I	CCATGG	2 cut(s)	323, 1485
BspACI	CCGC	4 cut(s)	426, 965, 1805, 1943
BspANI	GGCC	6 cut(s)	225, 429, 721, 1653, 1658, 1689
BspCNI	CTCAG	3 cut(s)	242, 673, 1537
BspDI	ATCGAT	1 cut(s)	879
BspFNI	CGCG	4 cut(s)	426, 1571, 1667, 1945
BspHI	TCATGA	3 cut(s)	984, 1372, 1378
BspLI	GGNNCC	2 cut(s)	1219, 1711
BspMAI	CTGCAG	1 cut(s)	994
BspPI	GGATC	6 cut(s)	315, 359, 537, 691, 1348, 1610
BspT104I	TTCGAA	1 cut(s)	1032
BspT107I	GGYRCC	1 cut(s)	1709
BspTNI	GGTCTC	1 cut(s)	1475
BsrDI	GCAATG	3 cut(s)	353, 516, 1839
BsrFI	RCCGGY	1 cut(s)	1262
BsrGI	TGTACA	3 cut(s)	603, 1720, 1935
BsrI	ACTGG	3 cut(s)	342, 722, 955
BssAI	RCCGGY	1 cut(s)	1262
BssNAI	GTATAC	1 cut(s)	831
BssNI	GRCGYC	1 cut(s)	1490
BssSI	CACGAG	1 cut(s)	1910
BssT1I	CCWWGG	2 cut(s)	323, 1485
Bst1107I	GTATAC	1 cut(s)	831
Bst2BI	CACGAG	1 cut(s)	1910
Bst2UI	CCWGG	6 cut(s)	455, 794, 1366, 1650, 1655, 1691
Bst4CI	ACNGT	7 cut(s)	218, 287, 445, 616, 1429, 1461, 1961
BstACI	GRCGYC	1 cut(s)	1490
BstAPI	GCANNNNNTGC	1 cut(s)	113
BstAUI	TGTACA	3 cut(s)	603, 1720, 1935
BstBI	TTCGAA	1 cut(s)	1032
BstC8I	GCNNGC	8 cut(s)	256, 628, 862, 866, 1068, 1285, 1542, 1943
BstDEI	CTNAG	7 cut(s)	229, 660, 1132, 1311, 1545, 1848, 1860
BstDSI	CCRYGG	2 cut(s)	323, 1485
BstENI	CCTNNNNNAGG	1 cut(s)	452
BstF5I	GGATG	3 cut(s)	244, 415, 655
BstFNI	CGCG	4 cut(s)	426, 1571, 1667, 1945
BstH2I	RGCGCY	1 cut(s)	1269
BstHHI	GCGC	3 cut(s)	426, 1268, 1947
BstMAI	GTCTC	1 cut(s)	1475
BstNI	CCWGG	6 cut(s)	455, 794, 1366, 1650, 1655, 1691
BstNSI	RCATGY	2 cut(s)	1735, 1936
BstSCI	CCNGG	7 cut(s)	453, 792, 1364, 1648, 1653, 1678, 1689
BstSFI	CTRYAG	2 cut(s)	990, 1948
BstUI	CGCG	4 cut(s)	426, 1571, 1667, 1945
BstV1I	GCAGC	8 cut(s)	119, 345, 776, 1372, 1754, 1843, 1856, 1916
BstV2I	GAAGAC	1 cut(s)	1087
BstX2I	RGATCY	3 cut(s)	529, 696, 1906
BstXI	CCANNNNNNTGG	1 cut(s)	638
BstYI	RGATCY	3 cut(s)	529, 696, 1906
BstZ17I	GTATAC	1 cut(s)	831
Bsu15I	ATCGAT	1 cut(s)	879
Bsu36I	CCTNAGG	1 cut(s)	660
BsuRI	GGCC	6 cut(s)	225, 429, 721, 1653, 1658, 1689
BsuTUI	ATCGAT	1 cut(s)	879
BtgI	CCRYGG	2 cut(s)	323, 1485
BtgZI	GCGATG	1 cut(s)	1521
BtrI	CACGTC	1 cut(s)	280
BtsCI	GGATG	3 cut(s)	244, 415, 655
BtsI	GCAGTG	1 cut(s)	1606
BtsIMutI	CAGTG	4 cut(s)	237, 349, 1434, 1606
Cac8I	GCNNGC	8 cut(s)	256, 628, 862, 866, 1068, 1285, 1542, 1943
CaiI	CAGNNNCTG	1 cut(s)	1748
CciI	TCATGA	3 cut(s)	984, 1372, 1378
CfoI	GCGC	3 cut(s)	426, 1268, 1947
Cfr10I	RCCGGY	1 cut(s)	1262
ClaI	ATCGAT	1 cut(s)	879
CseI	GACGC	1 cut(s)	1479
Csp6I	GTAC	8 cut(s)	283, 514, 604, 707, 1551, 1669, 1721, 1936
CspCI	CAANNNNNGTGG	2 cut(s)	202, 237
CviQI	GTAC	8 cut(s)	283, 514, 604, 707, 1551, 1669, 1721, 1936
DdeI	CTNAG	7 cut(s)	229, 660, 1132, 1311, 1545, 1848, 1860
DraIII	CACNNNGTG	1 cut(s)	232
DrdI	GACNNNNNNGTC	1 cut(s)	1489
DriI	GACNNNNNGTC	1 cut(s)	1581
DseDI	GACNNNNNNGTC	1 cut(s)	1489
EaeI	YGGCCR	3 cut(s)	223, 719, 1651
Eam1105I	GACNNNNNGTC	1 cut(s)	1581
Eco130I	CCWWGG	2 cut(s)	323, 1485
Eco24I	GRGCYC	2 cut(s)	1143, 1372
Eco31I	GGTCTC	1 cut(s)	1475
Eco32I	GATATC	2 cut(s)	1251, 1830
Eco57I	CTGAAG	5 cut(s)	446, 518, 840, 1578, 1770
Eco81I	CCTNAGG	1 cut(s)	660
EcoNI	CCTNNNNNAGG	1 cut(s)	452
EcoRI	GAATTC	1 cut(s)	980
EcoRII	CCWGG	6 cut(s)	453, 792, 1364, 1648, 1653, 1689
EcoRV	GATATC	2 cut(s)	1251, 1830
EcoT14I	CCWWGG	2 cut(s)	323, 1485
EcoT22I	ATGCAT	1 cut(s)	1057
EcoT38I	GRGCYC	2 cut(s)	1143, 1372
ErhI	CCWWGG	2 cut(s)	323, 1485
FalI	AAGNNNNNCTT	2 cut(s)	242, 274
FaqI	GGGAC	1 cut(s)	1329
FauI	CCCGC	2 cut(s)	1812, 1950
FbaI	TGATCA	2 cut(s)	1375, 1698
FblI	GTMKAC	1 cut(s)	830
Fnu4HI	GCNGC	9 cut(s)	108, 359, 427, 790, 1361, 1743, 1845, 1857, 1930
FokI	GGATG	3 cut(s)	251, 422, 642
FriOI	GRGCYC	2 cut(s)	1143, 1372
Fsp4HI	GCNGC	9 cut(s)	108, 359, 427, 790, 1361, 1743, 1845, 1857, 1930
FspBI	CTAG	4 cut(s)	776, 1022, 1337, 1643
GlaI	GCGC	3 cut(s)	425, 1267, 1946
GluI	GCNGC	9 cut(s)	108, 359, 427, 790, 1361, 1743, 1845, 1857, 1930
GsuI	CTGGAG	2 cut(s)	151, 1227
HaeII	RGCGCY	1 cut(s)	1269
HaeIII	GGCC	6 cut(s)	225, 429, 721, 1653, 1658, 1689
HapII	CCGG	4 cut(s)	722, 1221, 1263, 1680
HgaI	GACGC	1 cut(s)	1479
HhaI	GCGC	3 cut(s)	426, 1268, 1947
Hin1I	GRCGYC	1 cut(s)	1490
Hin6I	GCGC	3 cut(s)	424, 1266, 1945
HinP1I	GCGC	3 cut(s)	424, 1266, 1945
HindIII	AAGCTT	2 cut(s)	563, 1563
HinfI	GANTC	4 cut(s)	481, 805, 1084, 1307
HpaII	CCGG	4 cut(s)	722, 1221, 1263, 1680
HphI	GGTGA	5 cut(s)	299, 385, 632, 791, 1079
Hpy188I	TCNGA	6 cut(s)	179, 465, 842, 1750, 1774, 1823
HpyAV	CCTTC	8 cut(s)	394, 400, 440, 542, 682, 864, 1405, 1462
HpyCH4III	ACNGT	7 cut(s)	218, 287, 445, 616, 1429, 1461, 1961
HpyCH4IV	ACGT	2 cut(s)	67, 279
HpyF3I	CTNAG	7 cut(s)	229, 660, 1132, 1311, 1545, 1848, 1860
HpySE526I	ACGT	2 cut(s)	67, 279
Hsp92I	GRCGYC	1 cut(s)	1490
HspAI	GCGC	3 cut(s)	424, 1266, 1945
Ksp22I	TGATCA	2 cut(s)	1375, 1698
LmnI	GCTCC	7 cut(s)	179, 192, 457, 642, 760, 967, 1273
Lsp1109I	GCAGC	8 cut(s)	119, 345, 776, 1372, 1754, 1843, 1856, 1916
LweI	GCATC	5 cut(s)	400, 465, 664, 1130, 1393
MaeI	CTAG	4 cut(s)	776, 1022, 1337, 1643
MaeII	ACGT	2 cut(s)	67, 279
MaeIII	GTNAC	3 cut(s)	347, 748, 1955
MboII	GAAGA	9 cut(s)	476, 478, 524, 989, 1046, 1087, 1303, 1571, 1883
MflI	RGATCY	3 cut(s)	529, 696, 1906
MhlI	GDGCHC	2 cut(s)	1143, 1372
MlsI	TGGCCA	2 cut(s)	225, 1653
MluCI	AATT	3 cut(s)	980, 1003, 1157
MluI	ACGCGT	2 cut(s)	1569, 1665
MluNI	TGGCCA	2 cut(s)	225, 1653
MlyI	GAGTC	1 cut(s)	1078
MmeI	TCCRAC	5 cut(s)	157, 464, 793, 865, 1551
Mox20I	TGGCCA	2 cut(s)	225, 1653
Mph1103I	ATGCAT	1 cut(s)	1057
MroXI	GAANNNNTTC	1 cut(s)	591
MscI	TGGCCA	2 cut(s)	225, 1653
MseI	TTAA	4 cut(s)	933, 996, 1326, 1356
MslI	CAYNNNNRTG	2 cut(s)	521, 1377
Msp20I	TGGCCA	2 cut(s)	225, 1653
MspI	CCGG	4 cut(s)	722, 1221, 1263, 1680
MspR9I	CCNGG	7 cut(s)	455, 794, 1366, 1650, 1655, 1680, 1691
MvaI	CCWGG	6 cut(s)	455, 794, 1366, 1650, 1655, 1691
MvnI	CGCG	4 cut(s)	426, 1571, 1667, 1945
NciI	CCSGG	1 cut(s)	1680
NcoI	CCATGG	2 cut(s)	323, 1485
NlaIV	GGNNCC	2 cut(s)	1219, 1711
NmuCI	GTSAC	1 cut(s)	748
NsiI	ATGCAT	1 cut(s)	1057
NspI	RCATGY	2 cut(s)	1735, 1936
NspV	TTCGAA	1 cut(s)	1032
PagI	TCATGA	3 cut(s)	984, 1372, 1378
PasI	CCCWGGG	1 cut(s)	1365
PdmI	GAANNNNTTC	1 cut(s)	591
PfeI	GAWTC	3 cut(s)	481, 805, 1307
PflMI	CCANNNNNTGG	3 cut(s)	232, 617, 799
PkrI	GCNGC	9 cut(s)	109, 360, 428, 791, 1362, 1744, 1846, 1858, 1931
PleI	GAGTC	1 cut(s)	1078
PpsI	GAGTC	1 cut(s)	1078
PsiI	TTATAA	1 cut(s)	561
Psp6I	CCWGG	6 cut(s)	453, 792, 1364, 1648, 1653, 1689
PspGI	CCWGG	6 cut(s)	453, 792, 1364, 1648, 1653, 1689
PspN4I	GGNNCC	2 cut(s)	1219, 1711
PstI	CTGCAG	1 cut(s)	994
PstNI	CAGNNNCTG	1 cut(s)	1748
PsuI	RGATCY	3 cut(s)	529, 696, 1906
RsaI	GTAC	8 cut(s)	284, 515, 605, 708, 1552, 1670, 1722, 1937
RsaNI	GTAC	8 cut(s)	283, 514, 604, 707, 1551, 1669, 1721, 1936
RseI	CAYNNNNRTG	2 cut(s)	521, 1377
SaqAI	TTAA	4 cut(s)	933, 996, 1326, 1356
SatI	GCNGC	9 cut(s)	108, 359, 427, 790, 1361, 1743, 1845, 1857, 1930
ScaI	AGTACT	1 cut(s)	708
SchI	GAGTC	1 cut(s)	1078
ScrFI	CCNGG	7 cut(s)	455, 794, 1366, 1650, 1655, 1680, 1691
SduI	GDGCHC	2 cut(s)	1143, 1372
SfaNI	GCATC	5 cut(s)	400, 465, 664, 1130, 1393
SfcI	CTRYAG	2 cut(s)	990, 1948
SfuI	TTCGAA	1 cut(s)	1032
SmiMI	CAYNNNNRTG	2 cut(s)	521, 1377
SmlI	CTYRAG	1 cut(s)	1244
SmoI	CTYRAG	1 cut(s)	1244
Sse9I	AATT	3 cut(s)	980, 1003, 1157
SsiI	CCGC	4 cut(s)	426, 965, 1805, 1943
SspMI	CTAG	4 cut(s)	776, 1022, 1337, 1643
StyD4I	CCNGG	7 cut(s)	453, 792, 1364, 1648, 1653, 1678, 1689
StyI	CCWWGG	2 cut(s)	323, 1485
TaaI	ACNGT	7 cut(s)	218, 287, 445, 616, 1429, 1461, 1961
TaiI	ACGT	2 cut(s)	70, 282
TaqI	TCGA	6 cut(s)	166, 879, 975, 1032, 1585, 1897
TaqII	GACCGA	1 cut(s)	1865
TasI	AATT	3 cut(s)	980, 1003, 1157
TatI	WGTACW	5 cut(s)	603, 706, 1550, 1720, 1935
TauI	GCSGC	1 cut(s)	429
TfiI	GAWTC	3 cut(s)	481, 805, 1307
Tru1I	TTAA	4 cut(s)	933, 996, 1326, 1356
Tru9I	TTAA	4 cut(s)	933, 996, 1326, 1356
TscAI	CASTG	4 cut(s)	237, 349, 1434, 1606
TseFI	GTSAC	1 cut(s)	748
TseI	GCWGC	8 cut(s)	107, 358, 789, 1360, 1742, 1844, 1856, 1929
Tsp45I	GTSAC	1 cut(s)	748
TspDTI	ATGAA	5 cut(s)	510, 561, 973, 1395, 1884
TspGWI	ACGGA	1 cut(s)	492
TspRI	CASTG	4 cut(s)	237, 349, 1434, 1606
Van91I	CCANNNNNTGG	3 cut(s)	232, 617, 799
XagI	CCTNNNNNAGG	1 cut(s)	452
XapI	RAATTY	1 cut(s)	980
XceI	RCATGY	2 cut(s)	1735, 1936
XmiI	GTMKAC	1 cut(s)	830
XmnI	GAANNNNTTC	1 cut(s)	591
XspI	CTAG	4 cut(s)	776, 1022, 1337, 1643
ZrmI	AGTACT	1 cut(s)	708
Zsp2I	ATGCAT	1 cut(s)	1057

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.