Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr8

Orthogroup ID

OG0007010

Physical Location & Seq

Genomic Coordinates Reverse (-)

7515878 .. 7519997

Gene Structure 4120 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom08g09260.18

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2040 bp

ATGAAACCTACCTTGTTTTTAAAGCCCTCTTTAATCCCAAAAAGGAAGGAAAGTAAAACCAAATCCCACCTTTTATTCCCCTATAAAACGTGGGAGGTTTCTTGTTTTGCAAAACACAAAAAAGTTTGCAGCTTTTGCCTGTTTTGGTTTCTGGGTTGTTTTTGTTTTCTGGGTTTCATTGGAGGACTGCATTGTGATTTGCAGAGGAGAATAGTGGTGGGTTTTCGATTTGGAGATAGAAAAAAGGTCGGAGCTTTGATGGGGAGCGTAGTGGAGGAGATAAGCAACGGTGGTGGCCACTCAATGGTGGATGTGGAAAGTAAGGCTGGCTTTGGTGGAGGTCTTGTGGACGTGTACGGTGAGGATTGTGCCACCGAGGATCAGGTTCTCACACCATGGACTGCCTCTGTTGCCAGTGGTTACACATTGCTGCGTGATCCACACTACAACAAAGGTCTTGCCTTCACCGAGAAGGAGAGGGATGCACATTACTTGCGCGGCCTTCTGCCTCCAACCGTCCTAACCCAGGAGCTTCAGGAGAAGAAGGTGATGCAGAATCTTCGCCAATATGAAGTTCCGTTGCATAGGTACATTGCCATGATGGATCTTCAGGAGAGAAATGAAGGGCTGTTTTATAAGCTTTTGATTGACAATGTTGAGGAACTGCTTCCTGTTGTGTACACGCCAACAGTTGGCGAGGCTTGTCAAAAATATGGGAGCATTTTCAGGCATCCTCAGGGTCTTTACATCAGTTTGAAAGAGAAGGGAAAGATCCTTGAAGTACTAAAGAACTGGCCGGAGAGGGGTATTCAAGTTATTGTCGTGACTGATGGGGAGCGTATTTTGGGGCTAGGTGATCTTGGCTGCCAGGGCATGGGGATTCCTGTTGGAAAGCTTTCTTTGTATACTGCACTCGGAGGAGTTCCTCCTTCAGCGTGCTTGCCTATAACCATCGATGTTGGAACAAACAACGAGAAGTTGCTGAATGATGAGTTTTACATTGGGATTAAGCAAAGGAGAGCAACTGGACAGGAATATGCGGAGCTTCTCGAAGAATTCATGACTGCAGTTAAGCAGAATTATGGGGAGAAAGTCCTAGTTCAGTTTGAAGATTTTGCAAACCACAATGCATTTGAACTGCTGGCTAAATACAGCAAGACTCACCTTGTCTTCAATGATGACATTCAGGGAACGGCATCTGTGGTCTTAGCAGGGCTCGTCGCTTCCTTGAAATTACTTGGCGGAACATTAGCTGACCATACTTTCTTATTTCTGGGTGCTGGAGAGGCTGGAACCGGAATAGCAGAGCTTATAGCTCTCGAGATGTCAAAAAAGACCGGCGCTCCATTGAAAGAAGCTCGCAAGAAGATTTGGCTTGTGGATTCTAAGGGACTGATTGTTAAATCTCGCCTAGGATCACTTCAACACTTTAAGCAGCCTTGGGCTCATGATCATGAACCAATAAAGGAACTTGTAGACGCCGTGAAGGCAATCAAGCCAACAGTGCTGATAGGAACATCTGGTGTGGGAAAACAGTTCACGAAGGAGGTCGTTGAGACCATGGCCTCCTTGAATGAGAAACCACTTATCCTTGCTCTTTCCAACCCAACATCGCAAGCTGAGTGTACCGCTGAAGAAGCGTACACATGGACAAAGGGTCGAGCAATCTTTGGTAGTGGAAGTCCATTTGATCCTGTCAAATACGAGAACAAACTCCTAGTGCCTGGCCAGGCCAACAACGCGTACATATTCCCCGGTTTTGGCTTGGGATTGATCATGGCTGGTGCCATTCGTGTACACGATGACATGCTTTTGGCAGCCTCTGAAGCTCTGGCTACGCAAGTTTCCGAGGAACACTACGCCAAAGGAATGATCTACCCGCCATTTACCAACATCAGAAAGATATCAGCCAACATTGCAGCTAAGGTCGCTGCTAAGGTCTATGAACTCGGTCTGGCTTCTAATCTGCCTCGACCAAAAGATCTCGTCAAGTATGCGGAGAGCTGCATGTACAGCCCGCGCTACAGAAGTTACCGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004470 GO:0004473 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0005911 GO:0006081 GO:0006082 GO:0006090 GO:0006098 GO:0006108 GO:0006139 GO:0006725 GO:0006732 GO:0006733 GO:0006739 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009051 GO:0009117 GO:0009506 GO:0009507 GO:0009536 GO:0009987 GO:0010035 GO:0010038 GO:0016020 GO:0016043 GO:0016491 GO:0016614 GO:0016615 GO:0016616 GO:0019362 GO:0019637 GO:0019682 GO:0019693 GO:0019752 GO:0022607 GO:0030054 GO:0030312 GO:0031090 GO:0032787 GO:0034641 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043648 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044281 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046496 GO:0046686 GO:0048046 GO:0050896 GO:0051156 GO:0051186 GO:0051259 GO:0051260 GO:0055044 GO:0055086 GO:0055114 GO:0065003 GO:0071704 GO:0071840 GO:0071944 GO:0072524 GO:0098588 GO:0098805 GO:1901135 GO:1901360 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00620 ko00710 ko01100 ko01120 ko01200 map00620 map00710 map01100 map01120 map01200

Pfam Domains

Protein Families

Malic_M malic

Protein Analysis

680

Amino Acids

75.02

Weight (kDa)

8.48

Isoelectric Point (pI)

32.5

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007010)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G11670 AT5G25880 AT5G25880 AT5G25880
fragaria_vesca	FvH4_2g35330 FvH4_2g35330 FvH4_2g35330
malus_domestica	MD08G1111500.v1.1 MD15G1091000.v1.1
prunus_persica	Prupe.1G445400_v2.0.a1 Prupe.1G445400_v2.0.a1 Prupe.1G445400_v2.0.a1 Prupe.1G445400_v2.0.a1 Prupe.1G445400_v2.0.a1
pyrus_communis	pycom08g09260 pycom15g08500
rosa_chinensis	RchiOBHm_Chr6g0306591
rosa_laevigata	RLG00000010782
rosa_multiflora	Rmu_sc0017624.1_g000001
rosa_roxburghii	Rroxscaffold_7G00161630
rosa_rugosa	Rorug06G0350700 Rorug06G0350800
rosa_samantha	Rh6AG463100 Rh6BG437800
rosa_wichuraiana	Rw6G040350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	636
AccB1I	GGYRCC	1 cut(s)	1784
AccB7I	CCANNNNNTGG	3 cut(s)	304, 692, 874
AccI	GTMKAC	2 cut(s)	905, 1476
AccII	CGCG	3 cut(s)	498, 1742, 2020
AciI	CCGC	7 cut(s)	498, 1040, 1242, 1629, 1880, 1997, 2018
AclWI	GGATC	6 cut(s)	387, 431, 612, 766, 1423, 1685
AcoI	YGGCCR	3 cut(s)	295, 794, 1726
AcsI	RAATTY	1 cut(s)	1055
AcuI	CTGAAG	5 cut(s)	518, 593, 915, 1653, 1845
AcyI	GRCGYC	1 cut(s)	1479
AfaI	GTAC	9 cut(s)	356, 590, 680, 783, 1627, 1643, 1745, 1797, 2012
AfiI	CCNNNNNNNGG	6 cut(s)	304, 526, 692, 803, 874, 1760
AflIII	ACRYGT	2 cut(s)	351, 1740
AhdI	GACNNNNNGTC	1 cut(s)	1656
AjiI	CACGTC	1 cut(s)	352
AjnI	CCWGG	4 cut(s)	525, 867, 1723, 1728
AjuI	GAANNNNNNNTTGG	2 cut(s)	558, 590
Alw26I	GTCTC	1 cut(s)	1550
AlwI	GGATC	6 cut(s)	387, 431, 612, 766, 1423, 1685
AlwNI	CAGNNNCTG	1 cut(s)	1823
Ama87I	CYCGRG	1 cut(s)	1319
AoxI	GGCC	6 cut(s)	295, 499, 794, 1563, 1726, 1731
ApeKI	GCWGC	8 cut(s)	129, 430, 864, 1435, 1817, 1919, 1931, 2004
ApoI	RAATTY	1 cut(s)	1055
Asp700I	GAANNNNTTC	2 cut(s)	666, 895
AspA2I	CCTAGG	1 cut(s)	1411
AspLEI	GCGC	3 cut(s)	498, 1343, 2022
AsuC2I	CCSGG	1 cut(s)	1755
AsuHPI	GGTGA	5 cut(s)	371, 457, 559, 866, 1154
AvaI	CYCGRG	1 cut(s)	1319
AvrII	CCTAGG	1 cut(s)	1411
AxyI	CCTNAGG	1 cut(s)	735
BalI	TGGCCA	2 cut(s)	297, 1728
BanI	GGYRCC	1 cut(s)	1784
BanII	GRGCYC	2 cut(s)	1218, 1447
BbsI	GAAGAC	1 cut(s)	1162
BbvI	GCAGC	8 cut(s)	141, 417, 851, 1447, 1829, 1918, 1931, 1991
BccI	CCATC	4 cut(s)	253, 595, 824, 959
BceAI	ACGGC	2 cut(s)	1209, 1466
BcgI	CGANNNNNNTGC	2 cut(s)	542, 576
BciT130I	CCWGG	4 cut(s)	527, 869, 1725, 1730
BclI	TGATCA	2 cut(s)	1450, 1773
BcnI	CCSGG	1 cut(s)	1755
BcoDI	GTCTC	1 cut(s)	1550
BfaI	CTAG	4 cut(s)	851, 1097, 1412, 1718
BfmI	CTRYAG	2 cut(s)	1065, 2023
BfoI	RGCGCY	1 cut(s)	1344
BglI	GCCNNNNNGGC	1 cut(s)	1487
BglII	AGATCT	1 cut(s)	1981
BisI	GCNGC	9 cut(s)	130, 431, 499, 865, 1436, 1818, 1920, 1932, 2005
BlnI	CCTAGG	1 cut(s)	1411
BlsI	GCNGC	9 cut(s)	131, 432, 500, 866, 1437, 1819, 1921, 1933, 2006
BmcAI	AGTACT	1 cut(s)	783
Bme1390I	CCNGG	5 cut(s)	527, 869, 1725, 1730, 1755
BmeRI	GACNNNNNGTC	1 cut(s)	1656
BmeT110I	CYCGRG	1 cut(s)	1319
BmgBI	CACGTC	1 cut(s)	352
BmiI	GGNNCC	2 cut(s)	1294, 1786
BmrFI	CCNGG	5 cut(s)	527, 869, 1725, 1730, 1755
BmsI	GCATC	4 cut(s)	472, 540, 739, 1205
BpiI	GAAGAC	1 cut(s)	1162
BpmI	CTGGAG	1 cut(s)	1302
Bpu10I	CCTNAGC	2 cut(s)	1923, 1935
BpuMI	CCSGG	1 cut(s)	1755
Bsa29I	ATCGAT	1 cut(s)	954
BsaHI	GRCGYC	1 cut(s)	1479
BsaI	GGTCTC	1 cut(s)	1550
BsaJI	CCNNGG	9 cut(s)	375, 395, 525, 868, 1411, 1439, 1560, 1753, 1848
BsaWI	WCCGGW	1 cut(s)	1295
BsaXI	ACNNNNNCTCC	4 cut(s)	330, 360, 1327, 1357
Bsc4I	CCNNNNNNNGG	6 cut(s)	304, 526, 692, 803, 874, 1760
Bse118I	RCCGGY	1 cut(s)	1337
Bse1I	ACTGG	3 cut(s)	414, 797, 1030
Bse21I	CCTNAGG	1 cut(s)	735
Bse3DI	GCAATG	3 cut(s)	425, 591, 1914
BseBI	CCWGG	4 cut(s)	527, 869, 1725, 1730
BseCI	ATCGAT	1 cut(s)	954
BseDI	CCNNGG	9 cut(s)	375, 395, 525, 868, 1411, 1439, 1560, 1753, 1848
BseGI	GGATG	3 cut(s)	316, 487, 730
BseLI	CCNNNNNNNGG	6 cut(s)	304, 526, 692, 803, 874, 1760
BseMI	GCAATG	3 cut(s)	425, 591, 1914
BseMII	CTCAG	2 cut(s)	749, 1611
BseNI	ACTGG	3 cut(s)	414, 797, 1030
BseRI	GAGGAG	3 cut(s)	220, 290, 933
BseXI	GCAGC	8 cut(s)	141, 417, 851, 1447, 1829, 1918, 1931, 1991
BsgI	GTGCAG	1 cut(s)	894
Bsh1236I	CGCG	3 cut(s)	498, 1742, 2020
BshFI	GGCC	6 cut(s)	297, 501, 796, 1565, 1728, 1733
BshNI	GGYRCC	1 cut(s)	1784
BshVI	ATCGAT	1 cut(s)	954
BsiHKCI	CYCGRG	1 cut(s)	1319
BsiSI	CCGG	4 cut(s)	797, 1296, 1338, 1755
BslFI	GGGAC	1 cut(s)	1404
BslI	CCNNNNNNNGG	6 cut(s)	304, 526, 692, 803, 874, 1760
BsmAI	GTCTC	1 cut(s)	1550
BsmFI	GGGAC	1 cut(s)	1404
BsnI	GGCC	6 cut(s)	297, 501, 796, 1565, 1728, 1733
Bso31I	GGTCTC	1 cut(s)	1550
BsoBI	CYCGRG	1 cut(s)	1319
Bsp1286I	GDGCHC	2 cut(s)	1218, 1447
Bsp1407I	TGTACA	3 cut(s)	678, 1795, 2010
Bsp19I	CCATGG	2 cut(s)	395, 1560
BspACI	CCGC	7 cut(s)	498, 1040, 1242, 1629, 1880, 1997, 2018
BspANI	GGCC	6 cut(s)	297, 501, 796, 1565, 1728, 1733
BspCNI	CTCAG	2 cut(s)	748, 1612
BspDI	ATCGAT	1 cut(s)	954
BspFNI	CGCG	3 cut(s)	498, 1742, 2020
BspHI	TCATGA	3 cut(s)	1059, 1447, 1453
BspLI	GGNNCC	2 cut(s)	1294, 1786
BspMAI	CTGCAG	1 cut(s)	1069
BspPI	GGATC	6 cut(s)	387, 431, 612, 766, 1423, 1685
BspT107I	GGYRCC	1 cut(s)	1784
BspTNI	GGTCTC	1 cut(s)	1550
BsrDI	GCAATG	3 cut(s)	425, 591, 1914
BsrFI	RCCGGY	1 cut(s)	1337
BsrGI	TGTACA	3 cut(s)	678, 1795, 2010
BsrI	ACTGG	3 cut(s)	414, 797, 1030
BssAI	RCCGGY	1 cut(s)	1337
BssECI	CCNNGG	9 cut(s)	375, 395, 525, 868, 1411, 1439, 1560, 1753, 1848
BssNAI	GTATAC	1 cut(s)	906
BssNI	GRCGYC	1 cut(s)	1479
BssT1I	CCWWGG	4 cut(s)	395, 1411, 1439, 1560
Bst1107I	GTATAC	1 cut(s)	906
Bst2UI	CCWGG	4 cut(s)	527, 869, 1725, 1730
Bst4CI	ACNGT	7 cut(s)	290, 359, 517, 691, 1504, 1536, 2036
BstACI	GRCGYC	1 cut(s)	1479
BstAPI	GCANNNNNTGC	1 cut(s)	135
BstAUI	TGTACA	3 cut(s)	678, 1795, 2010
BstC8I	GCNNGC	7 cut(s)	328, 937, 941, 1143, 1360, 1617, 2018
BstDEI	CTNAG	6 cut(s)	735, 1207, 1386, 1620, 1923, 1935
BstDSI	CCRYGG	2 cut(s)	395, 1560
BstENI	CCTNNNNNAGG	1 cut(s)	524
BstF5I	GGATG	3 cut(s)	316, 487, 730
BstFNI	CGCG	3 cut(s)	498, 1742, 2020
BstH2I	RGCGCY	1 cut(s)	1344
BstHHI	GCGC	3 cut(s)	498, 1343, 2022
BstMAI	GTCTC	1 cut(s)	1550
BstNI	CCWGG	4 cut(s)	527, 869, 1725, 1730
BstNSI	RCATGY	2 cut(s)	1810, 2011
BstSCI	CCNGG	5 cut(s)	525, 867, 1723, 1728, 1753
BstSFI	CTRYAG	2 cut(s)	1065, 2023
BstUI	CGCG	3 cut(s)	498, 1742, 2020
BstV1I	GCAGC	8 cut(s)	141, 417, 851, 1447, 1829, 1918, 1931, 1991
BstV2I	GAAGAC	1 cut(s)	1162
BstX2I	RGATCY	3 cut(s)	604, 771, 1981
BstYI	RGATCY	3 cut(s)	604, 771, 1981
BstZ17I	GTATAC	1 cut(s)	906
Bsu15I	ATCGAT	1 cut(s)	954
Bsu36I	CCTNAGG	1 cut(s)	735
BsuRI	GGCC	6 cut(s)	297, 501, 796, 1565, 1728, 1733
BsuTUI	ATCGAT	1 cut(s)	954
BtgI	CCRYGG	2 cut(s)	395, 1560
BtgZI	GCGATG	1 cut(s)	1596
BtrI	CACGTC	1 cut(s)	352
BtsCI	GGATG	3 cut(s)	316, 487, 730
BtsIMutI	CAGTG	2 cut(s)	421, 1509
Cac8I	GCNNGC	7 cut(s)	328, 937, 941, 1143, 1360, 1617, 2018
CaiI	CAGNNNCTG	1 cut(s)	1823
CciI	TCATGA	3 cut(s)	1059, 1447, 1453
CfoI	GCGC	3 cut(s)	498, 1343, 2022
Cfr10I	RCCGGY	1 cut(s)	1337
ClaI	ATCGAT	1 cut(s)	954
CseI	GACGC	1 cut(s)	1487
Csp6I	GTAC	9 cut(s)	355, 589, 679, 782, 1626, 1642, 1744, 1796, 2011
CspCI	CAANNNNNGTGG	2 cut(s)	274, 309
CviQI	GTAC	9 cut(s)	355, 589, 679, 782, 1626, 1642, 1744, 1796, 2011
DdeI	CTNAG	6 cut(s)	735, 1207, 1386, 1620, 1923, 1935
DraI	TTTAAA	1 cut(s)	21
DriI	GACNNNNNGTC	1 cut(s)	1656
EaeI	YGGCCR	3 cut(s)	295, 794, 1726
Eam1105I	GACNNNNNGTC	1 cut(s)	1656
EciI	GGCGGA	1 cut(s)	1257
Eco130I	CCWWGG	4 cut(s)	395, 1411, 1439, 1560
Eco24I	GRGCYC	2 cut(s)	1218, 1447
Eco31I	GGTCTC	1 cut(s)	1550
Eco32I	GATATC	1 cut(s)	1905
Eco57I	CTGAAG	5 cut(s)	518, 593, 915, 1653, 1845
Eco81I	CCTNAGG	1 cut(s)	735
Eco88I	CYCGRG	1 cut(s)	1319
EcoNI	CCTNNNNNAGG	1 cut(s)	524
EcoRI	GAATTC	1 cut(s)	1055
EcoRII	CCWGG	4 cut(s)	525, 867, 1723, 1728
EcoRV	GATATC	1 cut(s)	1905
EcoT14I	CCWWGG	4 cut(s)	395, 1411, 1439, 1560
EcoT22I	ATGCAT	1 cut(s)	1132
EcoT38I	GRGCYC	2 cut(s)	1218, 1447
ErhI	CCWWGG	4 cut(s)	395, 1411, 1439, 1560
FalI	AAGNNNNNCTT	2 cut(s)	314, 346
FaqI	GGGAC	1 cut(s)	1404
FauI	CCCGC	2 cut(s)	1887, 2025
FbaI	TGATCA	2 cut(s)	1450, 1773
FblI	GTMKAC	2 cut(s)	905, 1476
Fnu4HI	GCNGC	9 cut(s)	130, 431, 499, 865, 1436, 1818, 1920, 1932, 2005
FokI	GGATG	3 cut(s)	323, 494, 717
FriOI	GRGCYC	2 cut(s)	1218, 1447
Fsp4HI	GCNGC	9 cut(s)	130, 431, 499, 865, 1436, 1818, 1920, 1932, 2005
FspBI	CTAG	4 cut(s)	851, 1097, 1412, 1718
GlaI	GCGC	3 cut(s)	497, 1342, 2021
GluI	GCNGC	9 cut(s)	130, 431, 499, 865, 1436, 1818, 1920, 1932, 2005
GsuI	CTGGAG	1 cut(s)	1302
HaeII	RGCGCY	1 cut(s)	1344
HaeIII	GGCC	6 cut(s)	297, 501, 796, 1565, 1728, 1733
HapII	CCGG	4 cut(s)	797, 1296, 1338, 1755
HgaI	GACGC	1 cut(s)	1487
HhaI	GCGC	3 cut(s)	498, 1343, 2022
Hin1I	GRCGYC	1 cut(s)	1479
Hin6I	GCGC	3 cut(s)	496, 1341, 2020
HinP1I	GCGC	3 cut(s)	496, 1341, 2020
HindIII	AAGCTT	2 cut(s)	638, 893
HinfI	GANTC	4 cut(s)	556, 880, 1159, 1382
HpaII	CCGG	4 cut(s)	797, 1296, 1338, 1755
HphI	GGTGA	5 cut(s)	371, 457, 559, 866, 1154
Hpy188I	TCNGA	5 cut(s)	251, 917, 1825, 1849, 1898
Hpy99I	CGWCG	1 cut(s)	1223
HpyCH4III	ACNGT	7 cut(s)	290, 359, 517, 691, 1504, 1536, 2036
HpyCH4IV	ACGT	2 cut(s)	89, 351
HpyF3I	CTNAG	6 cut(s)	735, 1207, 1386, 1620, 1923, 1935
HpySE526I	ACGT	2 cut(s)	89, 351
Hsp92I	GRCGYC	1 cut(s)	1479
HspAI	GCGC	3 cut(s)	496, 1341, 2020
Ksp22I	TGATCA	2 cut(s)	1450, 1773
LmnI	GCTCC	7 cut(s)	251, 264, 529, 717, 835, 1042, 1348
Lsp1109I	GCAGC	8 cut(s)	141, 417, 851, 1447, 1829, 1918, 1931, 1991
LweI	GCATC	4 cut(s)	472, 540, 739, 1205
MaeI	CTAG	4 cut(s)	851, 1097, 1412, 1718
MaeII	ACGT	2 cut(s)	89, 351
MaeIII	GTNAC	3 cut(s)	419, 823, 2030
MboII	GAAGA	8 cut(s)	551, 553, 599, 1064, 1121, 1162, 1378, 1646
MflI	RGATCY	3 cut(s)	604, 771, 1981
MhlI	GDGCHC	2 cut(s)	1218, 1447
MlsI	TGGCCA	2 cut(s)	297, 1728
MluCI	AATT	3 cut(s)	1055, 1078, 1232
MluI	ACGCGT	1 cut(s)	1740
MluNI	TGGCCA	2 cut(s)	297, 1728
MlyI	GAGTC	1 cut(s)	1153
MmeI	TCCRAC	5 cut(s)	229, 536, 868, 940, 1626
Mox20I	TGGCCA	2 cut(s)	297, 1728
Mph1103I	ATGCAT	1 cut(s)	1132
MroXI	GAANNNNTTC	2 cut(s)	666, 895
MscI	TGGCCA	2 cut(s)	297, 1728
MseI	TTAA	6 cut(s)	20, 32, 1008, 1071, 1401, 1431
MslI	CAYNNNNRTG	2 cut(s)	596, 1452
Msp20I	TGGCCA	2 cut(s)	297, 1728
MspA1I	CMGCKG	1 cut(s)	1631
MspI	CCGG	4 cut(s)	797, 1296, 1338, 1755
MspR9I	CCNGG	5 cut(s)	527, 869, 1725, 1730, 1755
MvaI	CCWGG	4 cut(s)	527, 869, 1725, 1730
MvnI	CGCG	3 cut(s)	498, 1742, 2020
NciI	CCSGG	1 cut(s)	1755
NcoI	CCATGG	2 cut(s)	395, 1560
NlaIV	GGNNCC	2 cut(s)	1294, 1786
NmuCI	GTSAC	1 cut(s)	823
NsiI	ATGCAT	1 cut(s)	1132
NspI	RCATGY	2 cut(s)	1810, 2011
PaeR7I	CTCGAG	1 cut(s)	1319
PagI	TCATGA	3 cut(s)	1059, 1447, 1453
PdmI	GAANNNNTTC	2 cut(s)	666, 895
PfeI	GAWTC	3 cut(s)	556, 880, 1382
PflMI	CCANNNNNTGG	3 cut(s)	304, 692, 874
PkrI	GCNGC	9 cut(s)	131, 432, 500, 866, 1437, 1819, 1921, 1933, 2006
PleI	GAGTC	1 cut(s)	1153
PpsI	GAGTC	1 cut(s)	1153
PsiI	TTATAA	1 cut(s)	636
Psp6I	CCWGG	4 cut(s)	525, 867, 1723, 1728
PspGI	CCWGG	4 cut(s)	525, 867, 1723, 1728
PspN4I	GGNNCC	2 cut(s)	1294, 1786
PstI	CTGCAG	1 cut(s)	1069
PstNI	CAGNNNCTG	1 cut(s)	1823
PsuI	RGATCY	3 cut(s)	604, 771, 1981
RsaI	GTAC	9 cut(s)	356, 590, 680, 783, 1627, 1643, 1745, 1797, 2012
RsaNI	GTAC	9 cut(s)	355, 589, 679, 782, 1626, 1642, 1744, 1796, 2011
RseI	CAYNNNNRTG	2 cut(s)	596, 1452
SaqAI	TTAA	6 cut(s)	20, 32, 1008, 1071, 1401, 1431
SatI	GCNGC	9 cut(s)	130, 431, 499, 865, 1436, 1818, 1920, 1932, 2005
ScaI	AGTACT	1 cut(s)	783
SchI	GAGTC	1 cut(s)	1153
ScrFI	CCNGG	5 cut(s)	527, 869, 1725, 1730, 1755
SduI	GDGCHC	2 cut(s)	1218, 1447
SfaNI	GCATC	4 cut(s)	472, 540, 739, 1205
SfcI	CTRYAG	2 cut(s)	1065, 2023
Sfr274I	CTCGAG	1 cut(s)	1319
SlaI	CTCGAG	1 cut(s)	1319
SmiMI	CAYNNNNRTG	2 cut(s)	596, 1452
SmlI	CTYRAG	1 cut(s)	1319
SmoI	CTYRAG	1 cut(s)	1319
Sse9I	AATT	3 cut(s)	1055, 1078, 1232
SsiI	CCGC	7 cut(s)	498, 1040, 1242, 1629, 1880, 1997, 2018
SspMI	CTAG	4 cut(s)	851, 1097, 1412, 1718
StyD4I	CCNGG	5 cut(s)	525, 867, 1723, 1728, 1753
StyI	CCWWGG	4 cut(s)	395, 1411, 1439, 1560
TaaI	ACNGT	7 cut(s)	290, 359, 517, 691, 1504, 1536, 2036
TaiI	ACGT	2 cut(s)	92, 354
TaqI	TCGA	6 cut(s)	226, 954, 1050, 1320, 1660, 1972
TaqII	GACCGA	1 cut(s)	1940
TasI	AATT	3 cut(s)	1055, 1078, 1232
TatI	WGTACW	4 cut(s)	678, 781, 1795, 2010
TauI	GCSGC	1 cut(s)	501
TfiI	GAWTC	3 cut(s)	556, 880, 1382
Tru1I	TTAA	6 cut(s)	20, 32, 1008, 1071, 1401, 1431
Tru9I	TTAA	6 cut(s)	20, 32, 1008, 1071, 1401, 1431
TscAI	CASTG	2 cut(s)	421, 1509
TseFI	GTSAC	1 cut(s)	823
TseI	GCWGC	8 cut(s)	129, 430, 864, 1435, 1817, 1919, 1931, 2004
Tsp45I	GTSAC	1 cut(s)	823
TspDTI	ATGAA	7 cut(s)	17, 166, 585, 636, 1048, 1470, 1959
TspGWI	ACGGA	1 cut(s)	567
TspRI	CASTG	2 cut(s)	421, 1509
Van91I	CCANNNNNTGG	3 cut(s)	304, 692, 874
XagI	CCTNNNNNAGG	1 cut(s)	524
XapI	RAATTY	1 cut(s)	1055
XceI	RCATGY	2 cut(s)	1810, 2011
XhoI	CTCGAG	1 cut(s)	1319
XmaJI	CCTAGG	1 cut(s)	1411
XmiI	GTMKAC	2 cut(s)	905, 1476
XmnI	GAANNNNTTC	2 cut(s)	666, 895
XspI	CTAG	4 cut(s)	851, 1097, 1412, 1718
ZrmI	AGTACT	1 cut(s)	783
Zsp2I	ATGCAT	1 cut(s)	1132

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.