Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000006

Orthogroup ID

OG0007010

Physical Location & Seq

Genomic Coordinates Forward (+)

50002447 .. 50009377

Gene Structure 6931 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug06G0350800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4800 bp

ATGGGCAGTGATTGGCCCTCTCCACCACATGTCCCTACGGGTGCCCCACGCAAGATTTTCTCTACAATTTCTCCACGTCTTGTCATCGAAAAACAAAATAATAATAATCTCTCCACGTCTCTTCGCTCAAGCCATATCATTCTGCACCAACCAGTACTCACCAAGTGGGAGGCCAAGTGCCACATAATCACCTTCAACCCCAGGATAGAAGAAGAAGATGTTAAACTAAGCAACCACAAAAGAGTGCAGCTTCGGCCTCCTCGCAAGACGATGGCTTCTGGGTCTTGGATCACGTCCACCTCGTGTTCCTCGTCCGTGATTCAATCCCAAGACGACACCTCAGCTCCGGCCATCTTCCAATGGCTGAGATTCGTCTTCCTCTCCCCCTGTCCCCAGAGAGCTCTGTTATCCTCCATTGATCTCTTGTTCCTGCTCACCCTCATCGCCTTCTCAATCCAAAAGCTCTACTCGAGGTTCGCCTCCCCCCGCGGCGGCAGCTCGGAACTCGATAAGCCGTTGATAACAAACAGCAGAGCTCGCCGTCCCGGAACGACGCTCTGTTTCAAGCTCTCTCTGACTCTCAGCGTTTTCTTGACACTCTGTTACACCGTAGTGTGCATTTTAGCGTTTACAAGAGGAAGTCCTGACTTCCCATGGAAGATCATAGATGGGTTGTTTTGGCTGGTTCAAGCTATAACTCATGGACTCATTACAGTACTAGTGGCACATGAAAAGAGATTTGAAGCTGTCAAGCACCCGCTTTCGCTTCGGATTTACTGGGTGGCCAATTTCGTTGCTGTTTCTCTGTTTGCAACTTCTGGAGTGATTCGTTTGGTTTACAATGAAGGAGGTTTGAGATTGGATGATGTAGTTTCCATTGTGTCTTTTCCTTTATCTGTTGTTCTTGCTGTTGTTGCGGTTAGAGGCTCCACCGGCATTAGAGTGATGAACAGAGAACAATCTAATGGAGGAGAAAACGGTGTGGAGACTAAATTGCATGAACCCCTCTTGATCAAATCCAATGTGACTGGTTTTGCTTCAGCTTCATTCATTAATAAAACCTTTTGGGTTTGGATGAATCCTCTGCTCAGTAAAGGGTACAAATCCCCTTTGAAGGTTGATGACGTTCCAACACTTGCTCCACAACACAGAGCCGAAAGAATGTCGGTGATTTTCGAATCCAACTGGCCTAAACCGGAGGAGAAGTCAGAGCATCCGGTTCGAACCACATTGCTCCGGTGCTTCTGGAAAGATATTGCTTTCACAGCTTCTCTTGCGGTGATCAGGCTGTGTGTCATGTATGTAGGGCCTGTGCTTATCCAGAGCTTTGTGGATTTCACTAATGGGAAACGAAGCTCGCCGTTTGAGGGATACTACCTTGTGTTGATTCTTCTGTGTGCCAAGTTCGTTGAGGTTTTGAGCACTCACCAATTCAACTTCAACTCACAGAAACTCGGCATGCTCATAAGATCAACTCTCATAACTTCTCTGTACAAGAAGGGCTTGAGGCTGACCTGCTCGGCGCGACAAGCTCATGGCGTTGGACAGATTGTGAATTACATGGCTGTGGATGCTCAGCAGCTCTCTGACATGATGCTGCAGCTTCATGCTATATGGATGATGCCGGTCCAGCTCATCATTGCTTTAAGCCTGCTGTACAACAACCTCGGTGCAGCTACAATCACAGCAGTGATTGGAATCATGGGTGTGATTGTGTTTGTGGTGTTTGGGACGAGAAGGAACAACAGGTTTCAGTTCAATCTGATGAAGCAAAGGGATTCGAGGATGAAGGCGACAAATGAGATGCTTAACTACATGAGGGTGATCAAGTTCCAGGCTTGGGAGGAACACTTCAACAAGAGGATTCAGACTTTTCGTGAGTCGGAGTTTAGCTGGCTGACCAAGTTCATGTACTCCATCTCGGCTAATGTTGTGCTCATGTGGTGCACTCCCATACTTATATCAACCCTCACATTTGCCACTGCCTTGTATTTGGGTGTCAAACTTGATGCTGGGACCGTGTTCACTACCACAACCATTTTCAAAATTCTGCAGGAGCCTATTAGGACATTCCCACAATCTATGATTTCAATTTCTCAAGCAATGATCTCGCTCGGAAGGTTGGACCGGTATATGAGTAGTAGGGAACTGGTGGAGGGTTCGGTTGAGAGAGAAGAAGGTTGTGATAGTAGTGTTGCTGTGGAGGTTAAGAATGGTGCATTTAGCTGGGATGATGAAAGTAATGAACAGGTTTTGAAGAATATAAACTTGACTGTCAACAAAGGTGAGCTAACCGCAATTGTTGGGACAGTAGGATCTGGAAAGTCTTCCCTGCTGGCCTCAATCCTTGGTGAGATGCACAAATTATCCGGAAAGGTAAAAGTTTGCGGGACAACTGCCTATGTGGCTCAAACATCATGGATTCAAAATGGGACAATTGAAGAAAACATCCTATTTGGTTTGCCAATGGACAGAGCGAGATACCAGGAAGTTATGAGGGTCTGTTGCTTGGAAAAGGACATGGAAATGATGGAGTATGGAGATCAAACTGAAATAGGAGAGCGCGGCATCAACCTAAGTGGTGGACAGAAACAAAGGATACAGCTTGCCAGGGCAGTATATCAGGACTGTGACATATATCTTCTTGATGATGTCTTCAGTGCTGTTGATGCTCATACCGGATCAGAAATATTCAAGGAATGTGTACGGGGAGTACTTAAGAACAAGACCATTTTACTTGTAACCCATCAAGTTGACTTCTTGCATAATGTTGATCTTATTGTGGTCATGAGAGAAGGAATGATAGTACAAGCAGGAAAGTACAACGATTTGTTAAGCCTCGATTTCAAAGCACTTGTAGTTGCGCACGAAAGCTCCATGGAGCTTGTAGAAATGAGCACAGCTATGCCTGGTGAAAGTACTTCTCCCAAACCACAGATATCTCGGCATTCCTCTTCAGAACATGGAGAAGCCAATGGCGAAAACAACTCTCAGCTGGTCGAACCCAAGTCCAAAGACAGAACATCTAAGCTCATCAAAGAAGAAGAGAAAGAATCAGGCAAAGTCAGCTTGCAGAATTACAAAATCTATTGCACAGAGGCTTTTGGATGGTGGGGTGTGGTGCTAGTGTTGTCTCTCTCTCTTTTGTGGCAAGGATCACTAATGGCTGGTGATTATTGGCTGGCCTATGAAACTTCAGCAAAGCGAGCATTGTCATTCGATCCTTCTGTGTTCATTACTGTCTATGCTATTATCGCAGCTGTTTCATTCTTCGTGGTATTGGTAAGAGCATTTTCTGTGACAATTGTGGGGCTTACAACAGCTCAAGTTTTCTTCAAGCAGATTCTTCACAGCATCCTTCATGCCCCCATGTCATTTTTCGATACTACACCTTCTGGAAGAATTTTGAGTAGGGCATCAACTGATCAGACGAATATCGACTTATTTCTTCCCTTCATTTTGGGCATTACCATTGCCATGTATATCACTGTGCTCGGTATCTTTATCGTCGTATGTCAAAACTCATGGCCAACAATCTTCTTACTCATACCACTTCTTTGGCTGAATATCTGGTACCGGGGTTACTATCTTGCATCATCTCGTGAATTAACTCGACTTGATTCAATCACAAAAGCGCCTGTTATCCATCATTTCTCAGAAAGCATATCTGGGGTTATGACAATCCGATCTTTCAGAAACCAGAACAAGTTTACCAAGGAAAATGTTAGGAGAGTGAATGCCAATTTGCGTATGGATTTCCACAATAACGGCTCCAATGAGTGGTTGGGATTCCGTCTAGAACTGCTTGGGAGCTTAATCCTCTGCATTTCCACTTTGTTCATGATCTTGCTGCCAAGCAGTATAGTAAAGCCAGAGAATATTGGCTTATCACTCTCCTATGGATTGTCCCTCAACGGTGTACTATTTTGGGCCATATATATGAGCTGCTTCGTCGAAAATAGGATGGTATCAGTTGAGAGGATAAAGCAGTTTTGTAACATTCCATCAGAAGCAGCATGGAAGATAGTAGATCGTGCCCCTCCTACGAATTGGCCAACTCATGGCAATGTTGAGCTGAAAGACTTGCAGGTCAGATATCGTCCGAATACTCCCTTGGTTCTCAAAGGCATTAGTCTGAGCATTCATGGGGGTGAGAAGGTCGGTGTTGTTGGTCGAACGGGGAGTGGGAAATCCACTTTAATTCAGGTGTTCTTTAGGCTGGTGGAACCTTCAGCGGGAAAAATTATCATCGATGGCATCGACATATGCACGATAGGGCTTCATGATCTCCGGTCTAGCTTTGGGATCATTCCTCAAGAACCTGTCCTTTTTGAAGGAACTGTGAGAAGCAACATTGATCCAATCGGGGTGTATTCAGATGAAGAGATTTGGAAGAGCTTGGAGCGCTGCCAACTTAAAGATGTTGTTGCTGCAAAACCCAAAAAACTCGATGCTTTAGTGGCCGATGATGGAGATAATTGGAGCGTGGGGCAAAGACAGCTTCTTTGTTTGGGACGAGTTATGTTAAAGCGCAGCAGGCTGTTGTTCATGGATGAGGCCACGGCTTCAGTTGACTCACAGACGGATGCTGCGATACAAAAGATCATCAGAGAGGACTTTGCTGCATGTACAATCATCAGCATTGCTCACAGAATACCAACAGTGATGGACTGCAATAGAGTTTTAGTAGTAGATGCAGGGAGGGCTAAAGAGTTTGACTCACCATCACACTTGCTCGAGAGGCCATCGCTGTTTGGGGCATTGGTTCAGGAGTATGCCAACCGCTCAGCAGGAATTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004470 GO:0004473 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0005911 GO:0006081 GO:0006082 GO:0006090 GO:0006098 GO:0006108 GO:0006139 GO:0006725 GO:0006732 GO:0006733 GO:0006739 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009051 GO:0009117 GO:0009506 GO:0009507 GO:0009536 GO:0009987 GO:0010035 GO:0010038 GO:0016020 GO:0016043 GO:0016491 GO:0016614 GO:0016615 GO:0016616 GO:0019362 GO:0019637 GO:0019682 GO:0019693 GO:0019752 GO:0022607 GO:0030054 GO:0030312 GO:0031090 GO:0032787 GO:0034641 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043648 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044281 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046496 GO:0046686 GO:0048046 GO:0050896 GO:0051156 GO:0051186 GO:0051259 GO:0051260 GO:0055044 GO:0055086 GO:0055114 GO:0065003 GO:0071704 GO:0071840 GO:0071944 GO:0072524 GO:0098588 GO:0098805 GO:1901135 GO:1901360 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00620 ko00710 ko01100 ko01120 ko01200 map00620 map00710 map01100 map01120 map01200

Pfam Domains

Protein Families

Malic_M malic

Protein Analysis

1599

Amino Acids

178.99

Weight (kDa)

8.86

Isoelectric Point (pI)

39.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ABC_membrane	PF00664	424 - 687	2e-25	ABC transporter transmembrane region
ABC_tran	PF00005	752 - 885	2.9e-21	ABC transporter
ABC_membrane	PF00664	1073 - 1293	1.8e-30	ABC transporter transmembrane region
ABC_tran	PF00005	1374 - 1522	4.4e-26	ABC transporter

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0007010)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G11670 AT5G25880 AT5G25880 AT5G25880
fragaria_vesca	FvH4_2g35330 FvH4_2g35330 FvH4_2g35330
malus_domestica	MD08G1111500.v1.1 MD15G1091000.v1.1
prunus_persica	Prupe.1G445400_v2.0.a1 Prupe.1G445400_v2.0.a1 Prupe.1G445400_v2.0.a1 Prupe.1G445400_v2.0.a1 Prupe.1G445400_v2.0.a1
pyrus_communis	pycom08g09260 pycom15g08500
rosa_chinensis	RchiOBHm_Chr6g0306591
rosa_laevigata	RLG00000010782
rosa_multiflora	Rmu_sc0017624.1_g000001
rosa_roxburghii	Rroxscaffold_7G00161630
rosa_rugosa	Rorug06G0350700 Rorug06G0350800
rosa_samantha	Rh6AG463100 Rh6BG437800
rosa_wichuraiana	Rw6G040350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	4088
Acc16I	TGCGCA	1 cut(s)	2865
Acc36I	ACCTGC	2 cut(s)	1523, 4078
Acc65I	GGTACC	1 cut(s)	3573
AccB1I	GGYRCC	2 cut(s)	41, 3573
AccB7I	CCANNNNNTGG	2 cut(s)	3780, 4061
AccBSI	CCGCTC	1 cut(s)	4785
AccII	CGCG	3 cut(s)	489, 1525, 2565
AccIII	TCCGGA	1 cut(s)	2369
AclWI	GGATC	7 cut(s)	296, 2323, 2689, 3163, 3215, 4313, 4352
AcoI	YGGCCR	5 cut(s)	348, 783, 3527, 4052, 4461
AcsI	RAATTY	3 cut(s)	2046, 3402, 4794
AcuI	CTGAAG	6 cut(s)	1023, 2641, 2940, 3180, 4215, 4551
AdeI	CACNNNGTG	2 cut(s)	165, 303
AfeI	AGCGCT	1 cut(s)	4406
AfiI	CCNNNNNNNGG	7 cut(s)	491, 1114, 1367, 1840, 3780, 4061, 4235
AflII	CTTAAG	1 cut(s)	2717
AflIII	ACRYGT	1 cut(s)	28
AgeI	ACCGGT	1 cut(s)	2127
AhlI	ACTAGT	1 cut(s)	718
AjiI	CACGTC	3 cut(s)	77, 117, 294
AjnI	CCWGG	5 cut(s)	200, 1833, 2484, 2609, 2908
AjuI	GAANNNNNNNTTGG	2 cut(s)	4097, 4129
AleI	CACNNNNGTG	2 cut(s)	611, 1688
Alw21I	GWGCWC	7 cut(s)	403, 538, 1424, 1938, 1949, 2900, 3495
Alw26I	GTCTC	3 cut(s)	123, 980, 3138
Alw44I	GTGCAC	1 cut(s)	1945
AlwI	GGATC	7 cut(s)	296, 2323, 2689, 3163, 3215, 4313, 4352
Ama87I	CYCGRG	2 cut(s)	469, 4736
Aor13HI	TCCGGA	1 cut(s)	2369
Aor51HI	AGCGCT	1 cut(s)	4406
ApaLI	GTGCAC	1 cut(s)	1945
ApoI	RAATTY	3 cut(s)	2046, 3402, 4794
ArsI	GACNNNNNNTTYG	2 cut(s)	4488, 4520
AseI	ATTAAT	1 cut(s)	1053
AsiGI	ACCGGT	1 cut(s)	2127
Asp700I	GAANNNNTTC	1 cut(s)	2326
Asp718I	GGTACC	1 cut(s)	3573
AspLEI	GCGC	6 cut(s)	1525, 2565, 2866, 3637, 4407, 4533
AspS9I	GGNCC	6 cut(s)	15, 1307, 1627, 2016, 2125, 3930
AsuC2I	CCSGG	2 cut(s)	546, 3578
AsuII	TTCGAA	2 cut(s)	1176, 1222
AvaI	CYCGRG	2 cut(s)	469, 4736
AvaII	GGWCC	3 cut(s)	1627, 2016, 2125
BaeGI	GKGCMC	3 cut(s)	46, 1949, 4039
BaeI	ACNNNNGTAYC	2 cut(s)	3565, 3598
BalI	TGGCCA	3 cut(s)	785, 3529, 4054
BanI	GGYRCC	2 cut(s)	41, 3573
BanII	GRGCYC	2 cut(s)	403, 538
BauI	CACGAG	2 cut(s)	301, 3600
BbsI	GAAGAC	3 cut(s)	367, 2319, 2647
Bbv12I	GWGCWC	7 cut(s)	403, 538, 1424, 1938, 1949, 2900, 3495
BbvCI	CCTCAGC	1 cut(s)	340
BceAI	ACGGC	5 cut(s)	499, 525, 1345, 3784, 4578
BcgI	CGANNNNNNTGC	4 cut(s)	1202, 1236, 2091, 2125
BciT130I	CCWGG	5 cut(s)	202, 1835, 2486, 2611, 2910
BciVI	GTATCC	2 cut(s)	1364, 2592
BclI	TGATCA	4 cut(s)	1011, 1281, 1824, 3424
BcnI	CCSGG	2 cut(s)	546, 3578
BcoDI	GTCTC	3 cut(s)	123, 980, 3138
BcuI	ACTAGT	1 cut(s)	718
BfaI	CTAG	4 cut(s)	719, 3125, 3797, 4296
BfmI	CTRYAG	2 cut(s)	1598, 2051
BfoI	RGCGCY	2 cut(s)	3638, 4408
BfrI	CTTAAG	1 cut(s)	2717
BfuAI	ACCTGC	2 cut(s)	1523, 4078
BfuI	GTATCC	2 cut(s)	1364, 2592
BglI	GCCNNNNNGGC	1 cut(s)	2405
BlpI	GCTNAGC	2 cut(s)	1575, 4786
BmcAI	AGTACT	4 cut(s)	156, 717, 2715, 2920
Bme1390I	CCNGG	7 cut(s)	202, 546, 1835, 2486, 2611, 2910, 3578
Bme18I	GGWCC	3 cut(s)	1627, 2016, 2125
BmeT110I	CYCGRG	2 cut(s)	469, 4736
BmgBI	CACGTC	3 cut(s)	77, 117, 294
BmgT120I	GGNCC	6 cut(s)	15, 1307, 1627, 2016, 2125, 3930
BmiI	GGNNCC	7 cut(s)	43, 928, 2017, 2058, 3575, 3772, 4227
BmrFI	CCNGG	7 cut(s)	202, 546, 1835, 2486, 2611, 2910, 3578
BmrI	ACTGGG	1 cut(s)	787
BmuI	ACTGGG	1 cut(s)	787
BpiI	GAAGAC	3 cut(s)	367, 2319, 2647
BplI	GAGNNNNNCTC	2 cut(s)	4703, 4735
BpmI	CTGGAG	1 cut(s)	840
Bpu10I	CCTNAGC	1 cut(s)	340
Bpu1102I	GCTNAGC	2 cut(s)	1575, 4786
Bpu14I	TTCGAA	2 cut(s)	1176, 1222
BpuEI	CTTGAG	5 cut(s)	112, 1525, 2082, 3309, 4299
BpuMI	CCSGG	2 cut(s)	546, 3578
Bsa29I	ATCGAT	1 cut(s)	4251
BsaBI	GATNNNNATC	1 cut(s)	1697
BsaWI	WCCGGW	7 cut(s)	1195, 1216, 1236, 2127, 2369, 2678, 4290
Bsc4I	CCNNNNNNNGG	7 cut(s)	491, 1114, 1367, 1840, 3780, 4061, 4235
Bse118I	RCCGGY	3 cut(s)	932, 1624, 2127
Bse1I	ACTGG	5 cut(s)	152, 782, 1033, 1190, 2154
Bse3DI	GCAATG	6 cut(s)	1229, 1638, 2109, 3471, 4072, 4641
Bse8I	GATNNNNATC	1 cut(s)	1697
BseAI	TCCGGA	1 cut(s)	2369
BseBI	CCWGG	5 cut(s)	202, 1835, 2486, 2611, 2910
BseCI	ATCGAT	1 cut(s)	4251
BseJI	GATNNNNATC	1 cut(s)	1697
BseLI	CCNNNNNNNGG	7 cut(s)	491, 1114, 1367, 1840, 3780, 4061, 4235
BseMI	GCAATG	6 cut(s)	1229, 1638, 2109, 3471, 4072, 4641
BseMII	CTCAG	9 cut(s)	354, 356, 595, 1102, 1589, 3005, 3669, 4127, 4800
BseNI	ACTGG	5 cut(s)	152, 782, 1033, 1190, 2154
BseRI	GAGGAG	3 cut(s)	249, 984, 1214
BseSI	GKGCMC	3 cut(s)	46, 1949, 4039
BseYI	CCCAGC	2 cut(s)	2012, 2226
BsgI	GTGCAG	3 cut(s)	128, 266, 1692
Bsh1236I	CGCG	3 cut(s)	489, 1525, 2565
BshNI	GGYRCC	2 cut(s)	41, 3573
BshTI	ACCGGT	1 cut(s)	2127
BshVI	ATCGAT	1 cut(s)	4251
BsiHKAI	GWGCWC	7 cut(s)	403, 538, 1424, 1938, 1949, 2900, 3495
BsiHKCI	CYCGRG	2 cut(s)	469, 4736
BslI	CCNNNNNNNGG	7 cut(s)	491, 1114, 1367, 1840, 3780, 4061, 4235
BsmAI	GTCTC	3 cut(s)	123, 980, 3138
BsmBI	CGTCTC	1 cut(s)	123
BsmI	GAATGC	3 cut(s)	2947, 3742, 4140
BsoBI	CYCGRG	2 cut(s)	469, 4736
Bsp119I	TTCGAA	2 cut(s)	1176, 1222
Bsp1286I	GDGCHC	9 cut(s)	46, 403, 538, 1424, 1938, 1949, 2900, 3495, 4039
Bsp13I	TCCGGA	1 cut(s)	2369
Bsp1407I	TGTACA	3 cut(s)	1491, 1656, 4628
Bsp1720I	GCTNAGC	2 cut(s)	1575, 4786
Bsp19I	CCATGG	2 cut(s)	653, 2877
BspCNI	CTCAG	9 cut(s)	353, 357, 594, 1101, 1588, 3004, 3668, 4128, 4799
BspDI	ATCGAT	1 cut(s)	4251
BspEI	TCCGGA	1 cut(s)	2369
BspFNI	CGCG	3 cut(s)	489, 1525, 2565
BspHI	TCATGA	3 cut(s)	2787, 3840, 4282
BspLI	GGNNCC	7 cut(s)	43, 928, 2017, 2058, 3575, 3772, 4227
BspMAI	CTGCAG	2 cut(s)	1602, 2055
BspMI	ACCTGC	2 cut(s)	1523, 4078
BspPI	GGATC	7 cut(s)	296, 2323, 2689, 3163, 3215, 4313, 4352
BspQI	GCTCTTC	1 cut(s)	4388
BspT104I	TTCGAA	2 cut(s)	1176, 1222
BspT107I	GGYRCC	2 cut(s)	41, 3573
BspTI	CTTAAG	1 cut(s)	2717
BsrBI	CCGCTC	1 cut(s)	4785
BsrDI	GCAATG	6 cut(s)	1229, 1638, 2109, 3471, 4072, 4641
BsrFI	RCCGGY	3 cut(s)	932, 1624, 2127
BsrGI	TGTACA	3 cut(s)	1491, 1656, 4628
BsrI	ACTGG	5 cut(s)	152, 782, 1033, 1190, 2154
BssAI	RCCGGY	3 cut(s)	932, 1624, 2127
BssSI	CACGAG	2 cut(s)	301, 3600
BssT1I	CCWWGG	5 cut(s)	653, 2347, 2877, 3714, 4113
Bst2BI	CACGAG	2 cut(s)	301, 3600
Bst2UI	CCWGG	5 cut(s)	202, 1835, 2486, 2611, 2910
Bst6I	CTCTTC	5 cut(s)	126, 2959, 3039, 4377, 4388
BstAFI	CTTAAG	1 cut(s)	2717
BstAUI	TGTACA	3 cut(s)	1491, 1656, 4628
BstBI	TTCGAA	2 cut(s)	1176, 1222
BstDSI	CCRYGG	4 cut(s)	487, 653, 2877, 4560
BstFNI	CGCG	3 cut(s)	489, 1525, 2565
BstH2I	RGCGCY	2 cut(s)	3638, 4408
BstHHI	GCGC	6 cut(s)	1525, 2565, 2866, 3637, 4407, 4533
BstMAI	GTCTC	3 cut(s)	123, 980, 3138
BstNI	CCWGG	5 cut(s)	202, 1835, 2486, 2611, 2910
BstNSI	RCATGY	3 cut(s)	32, 1462, 4629
BstSCI	CCNGG	7 cut(s)	200, 544, 1833, 2484, 2609, 2908, 3576
BstSFI	CTRYAG	2 cut(s)	1598, 2051
BstSLI	GKGCMC	3 cut(s)	46, 1949, 4039
BstUI	CGCG	3 cut(s)	489, 1525, 2565
BstV2I	GAAGAC	3 cut(s)	367, 2319, 2647
BstX2I	RGATCY	1 cut(s)	2315
BstXI	CCANNNNNNTGG	1 cut(s)	3558
BstYI	RGATCY	1 cut(s)	2315
Bsu15I	ATCGAT	1 cut(s)	4251
BsuI	GTATCC	2 cut(s)	1364, 2592
BsuTUI	ATCGAT	1 cut(s)	4251
BtgI	CCRYGG	4 cut(s)	487, 653, 2877, 4560
BtgZI	GCGATG	2 cut(s)	427, 4731
BtrI	CACGTC	3 cut(s)	77, 117, 294
BtsI	GCAGTG	3 cut(s)	13, 1695, 1980
BtsIMutI	CAGTG	6 cut(s)	13, 1695, 1980, 2665, 3486, 4668
BveI	ACCTGC	2 cut(s)	1523, 4078
CciI	TCATGA	3 cut(s)	2787, 3840, 4282
CfoI	GCGC	6 cut(s)	1525, 2565, 2866, 3637, 4407, 4533
Cfr10I	RCCGGY	3 cut(s)	932, 1624, 2127
Cfr13I	GGNCC	6 cut(s)	15, 1307, 1627, 2016, 2125, 3930
Cfr42I	CCGCGG	1 cut(s)	490
ClaI	ATCGAT	1 cut(s)	4251
CseI	GACGC	1 cut(s)	562
CspAI	ACCGGT	1 cut(s)	2127
CspCI	CAANNNNNGTGG	2 cut(s)	2560, 2595
DraIII	CACNNNGTG	2 cut(s)	165, 303
DrdI	GACNNNNNNGTC	1 cut(s)	4088
DseDI	GACNNNNNNGTC	1 cut(s)	4088
EaeI	YGGCCR	5 cut(s)	348, 783, 3527, 4052, 4461
Eam1104I	CTCTTC	5 cut(s)	126, 2959, 3039, 4377, 4388
EarI	CTCTTC	5 cut(s)	126, 2959, 3039, 4377, 4388
Ecl136II	GAGCTC	2 cut(s)	401, 536
Eco130I	CCWWGG	5 cut(s)	653, 2347, 2877, 3714, 4113
Eco24I	GRGCYC	2 cut(s)	403, 538
Eco32I	GATATC	2 cut(s)	2940, 4097
Eco47I	GGWCC	3 cut(s)	1627, 2016, 2125
Eco47III	AGCGCT	1 cut(s)	4406
Eco53kI	GAGCTC	2 cut(s)	401, 536
Eco57I	CTGAAG	6 cut(s)	1023, 2641, 2940, 3180, 4215, 4551
Eco88I	CYCGRG	2 cut(s)	469, 4736
EcoICRI	GAGCTC	2 cut(s)	401, 536
EcoO109I	RGGNCCY	1 cut(s)	1307
EcoRII	CCWGG	5 cut(s)	200, 1833, 2484, 2609, 2908
EcoRV	GATATC	2 cut(s)	2940, 4097
EcoT14I	CCWWGG	5 cut(s)	653, 2347, 2877, 3714, 4113
EcoT38I	GRGCYC	2 cut(s)	403, 538
ErhI	CCWWGG	5 cut(s)	653, 2347, 2877, 3714, 4113
Esp3I	CGTCTC	1 cut(s)	123
FalI	AAGNNNNNCTT	2 cut(s)	1487, 1519
FauI	CCCGC	4 cut(s)	494, 765, 2381, 4228
FauNDI	CATATG	1 cut(s)	4265
FbaI	TGATCA	4 cut(s)	1011, 1281, 1824, 3424
FriOI	GRGCYC	2 cut(s)	403, 538
FspBI	CTAG	4 cut(s)	719, 3125, 3797, 4296
FspI	TGCGCA	1 cut(s)	2865
GlaI	GCGC	6 cut(s)	1524, 2564, 2865, 3636, 4406, 4532
GsaI	CCCAGC	2 cut(s)	2016, 2230
GsuI	CTGGAG	1 cut(s)	840
HaeII	RGCGCY	2 cut(s)	3638, 4408
HgaI	GACGC	1 cut(s)	562
HhaI	GCGC	6 cut(s)	1525, 2565, 2866, 3637, 4407, 4533
Hin6I	GCGC	6 cut(s)	1523, 2563, 2864, 3635, 4405, 4531
HinP1I	GCGC	6 cut(s)	1523, 2563, 2864, 3635, 4405, 4531
HincII	GTYRAC	3 cut(s)	2278, 2755, 4573
HindII	GTYRAC	3 cut(s)	2278, 2755, 4573
Hpy99I	CGWCG	3 cut(s)	556, 3512, 3956
HpyCH4IV	ACGT	4 cut(s)	76, 116, 293, 1125
HpySE526I	ACGT	4 cut(s)	76, 116, 293, 1125
HspAI	GCGC	6 cut(s)	1523, 2563, 2864, 3635, 4405, 4531
Kpn2I	TCCGGA	1 cut(s)	2369
KpnI	GGTACC	1 cut(s)	3577
Ksp22I	TGATCA	4 cut(s)	1011, 1281, 1824, 3424
KspI	CCGCGG	1 cut(s)	490
LguI	GCTCTTC	1 cut(s)	4388
MaeI	CTAG	4 cut(s)	719, 3125, 3797, 4296
MaeII	ACGT	4 cut(s)	76, 116, 293, 1125
MaeIII	GTNAC	7 cut(s)	602, 1024, 2630, 2740, 3298, 3581, 3995
MbiI	CCGCTC	1 cut(s)	4785
MfeI	CAATTG	3 cut(s)	2298, 2436, 3303
MflI	RGATCY	1 cut(s)	2315
MhlI	GDGCHC	9 cut(s)	46, 403, 538, 1424, 1938, 1949, 2900, 3495, 4039
MlsI	TGGCCA	3 cut(s)	785, 3529, 4054
MluNI	TGGCCA	3 cut(s)	785, 3529, 4054
MlyI	GAGTC	5 cut(s)	571, 699, 1889, 4568, 4712
MmeI	TCCRAC	5 cut(s)	1154, 1206, 1522, 1863, 2103
Mox20I	TGGCCA	3 cut(s)	785, 3529, 4054
MroI	TCCGGA	1 cut(s)	2369
MroXI	GAANNNNTTC	1 cut(s)	2326
MscI	TGGCCA	3 cut(s)	785, 3529, 4054
Msp20I	TGGCCA	3 cut(s)	785, 3529, 4054
MspA1I	CMGCKG	4 cut(s)	489, 2995, 3260, 4235
MspCI	CTTAAG	1 cut(s)	2717
MspR9I	CCNGG	7 cut(s)	202, 546, 1835, 2486, 2611, 2910, 3578
MunI	CAATTG	3 cut(s)	2298, 2436, 3303
Mva1269I	GAATGC	3 cut(s)	2947, 3742, 4140
MvaI	CCWGG	5 cut(s)	202, 1835, 2486, 2611, 2910
MvnI	CGCG	3 cut(s)	489, 1525, 2565
NciI	CCSGG	2 cut(s)	546, 3578
NcoI	CCATGG	2 cut(s)	653, 2877
NdeI	CATATG	1 cut(s)	4265
NlaIV	GGNNCC	7 cut(s)	43, 928, 2017, 2058, 3575, 3772, 4227
NmeAIII	GCCGAG	4 cut(s)	1434, 1499, 1901, 2923
NmuCI	GTSAC	3 cut(s)	1024, 2630, 3298
NsbI	TGCGCA	1 cut(s)	2865
NspI	RCATGY	3 cut(s)	32, 1462, 4629
NspV	TTCGAA	2 cut(s)	1176, 1222
OliI	CACNNNNGTG	2 cut(s)	611, 1688
PaeI	GCATGC	1 cut(s)	1462
PaeR7I	CTCGAG	2 cut(s)	469, 4736
PagI	TCATGA	3 cut(s)	2787, 3840, 4282
PciI	ACATGT	1 cut(s)	28
PciSI	GCTCTTC	1 cut(s)	4388
PcsI	WCGNNNNNNNCGW	4 cut(s)	299, 308, 4257, 4589
PctI	GAATGC	3 cut(s)	2947, 3742, 4140
PdmI	GAANNNNTTC	1 cut(s)	2326
PflMI	CCANNNNNTGG	2 cut(s)	3780, 4061
PfoI	TCCNGGA	1 cut(s)	544
PinAI	ACCGGT	1 cut(s)	2127
PleI	GAGTC	5 cut(s)	571, 699, 1888, 4568, 4712
PpsI	GAGTC	5 cut(s)	571, 699, 1888, 4568, 4712
PscI	ACATGT	1 cut(s)	28
PshBI	ATTAAT	1 cut(s)	1053
Psp124BI	GAGCTC	2 cut(s)	403, 538
Psp6I	CCWGG	5 cut(s)	200, 1833, 2484, 2609, 2908
PspFI	CCCAGC	2 cut(s)	2012, 2226
PspGI	CCWGG	5 cut(s)	200, 1833, 2484, 2609, 2908
PspN4I	GGNNCC	7 cut(s)	43, 928, 2017, 2058, 3575, 3772, 4227
PspPI	GGNCC	6 cut(s)	15, 1307, 1627, 2016, 2125, 3930
PspXI	VCTCGAGB	1 cut(s)	469
PstI	CTGCAG	2 cut(s)	1602, 2055
PsuI	RGATCY	1 cut(s)	2315
PvuII	CAGCTG	2 cut(s)	2995, 3260
SacI	GAGCTC	2 cut(s)	403, 538
SacII	CCGCGG	1 cut(s)	490
SapI	GCTCTTC	1 cut(s)	4388
Sau96I	GGNCC	6 cut(s)	15, 1307, 1627, 2016, 2125, 3930
ScaI	AGTACT	4 cut(s)	156, 717, 2715, 2920
SchI	GAGTC	5 cut(s)	571, 699, 1889, 4568, 4712
ScrFI	CCNGG	7 cut(s)	202, 546, 1835, 2486, 2611, 2910, 3578
SduI	GDGCHC	9 cut(s)	46, 403, 538, 1424, 1938, 1949, 2900, 3495, 4039
SfcI	CTRYAG	2 cut(s)	1598, 2051
Sfr274I	CTCGAG	2 cut(s)	469, 4736
Sfr303I	CCGCGG	1 cut(s)	490
SfuI	TTCGAA	2 cut(s)	1176, 1222
SgrBI	CCGCGG	1 cut(s)	490
SinI	GGWCC	3 cut(s)	1627, 2016, 2125
SlaI	CTCGAG	2 cut(s)	469, 4736
SmlI	CTYRAG	8 cut(s)	127, 469, 1504, 2097, 2717, 3324, 4314, 4736
SmoI	CTYRAG	8 cut(s)	127, 469, 1504, 2097, 2717, 3324, 4314, 4736
SpeI	ACTAGT	1 cut(s)	718
SphI	GCATGC	1 cut(s)	1462
SspI	AATATT	2 cut(s)	2691, 3880
SspMI	CTAG	4 cut(s)	719, 3125, 3797, 4296
SstI	GAGCTC	2 cut(s)	403, 538
StyD4I	CCNGG	7 cut(s)	200, 544, 1833, 2484, 2609, 2908, 3576
StyI	CCWWGG	5 cut(s)	653, 2347, 2877, 3714, 4113
TaiI	ACGT	4 cut(s)	79, 119, 296, 1128
TauI	GCSGC	3 cut(s)	492, 495, 2568
TscAI	CASTG	6 cut(s)	13, 1695, 1987, 2665, 3493, 4668
TseFI	GTSAC	3 cut(s)	1024, 2630, 3298
Tsp45I	GTSAC	3 cut(s)	1024, 2630, 3298
TspGWI	ACGGA	3 cut(s)	304, 3782, 4598
TspRI	CASTG	6 cut(s)	13, 1695, 1987, 2665, 3493, 4668
Van91I	CCANNNNNTGG	2 cut(s)	3780, 4061
Vha464I	CTTAAG	1 cut(s)	2717
VneI	GTGCAC	1 cut(s)	1945
VpaK11BI	GGWCC	3 cut(s)	1627, 2016, 2125
VspI	ATTAAT	1 cut(s)	1053
XapI	RAATTY	3 cut(s)	2046, 3402, 4794
XbaI	TCTAGA	1 cut(s)	3796
XceI	RCATGY	3 cut(s)	32, 1462, 4629
XcmI	CCANNNNNNNNNTGG	2 cut(s)	3748, 3781
XhoI	CTCGAG	2 cut(s)	469, 4736
XmnI	GAANNNNTTC	1 cut(s)	2326
XspI	CTAG	4 cut(s)	719, 3125, 3797, 4296
ZrmI	AGTACT	4 cut(s)	156, 717, 2715, 2920

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.