Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0007010

Physical Location & Seq

Genomic Coordinates Reverse (-)

6302898 .. 6307658

Gene Structure 4761 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1091000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1782 bp

ATGGGGAGCGTAGTGGAGGAGATAAGGAACGGTGGTGGCCACTCAGTGGTGGATGTGGAAAGTAAGGTTGGCTTTGGTGGAGGTATTGAGGACGTGTACGGTGAGGATTGTGCCACGGAAGATCAGGTTCTCACACCATGGACTGCCTCGGTTGCTAGTGGATACACATTGCTGCGTGATCCGCACTACAACAAAGGACTTGCCTTCACAGAGAAGGAGAGAGATGCGCATTACTTGCGCGGCCTTCTGCCTCCAGCCGTCCTGACTCAGGAGCTTCAGGAGAAGAAGTTGATGCAAAATCTTCGCCAATATGAAGTTCCGTTGCATAGGTACATTGCAATGATGGATCTTCAGGAGAGAAATGAAGGACTGTTTTATAAGCTTTTGATTGATAATGTTGAGGAGCTGCTTCCTGTCGTGTACACGCCAACAGTTGGTGAGGCTTGCCAAAAATATGGGAGCATTTTCAGGCATCCACAGGGTCTTTATATCAGTTTGAAAGAGAAGGGAAAGATCCTTGAAGTACTAAAGAACTGGCCACAGAGGGGTATTCAAGTTATTGTCGTGACTGATGGTGAGCGTATTTTGGGGCTCGGGGATCTTGGCTGCCAGGGCATGGGGATTCCTGTTGGAAAGCTCTCTTTGTATACTGCACTCGGAGGAGTTCCTCCTTCAGCCTGCTTGCCTATAACCATCGATGTTGGCACAAACAACGAGAAGTTGCTGAATGATGAGTTTTACATTGGGCTAAAGCAAAGGAGAGCAACTGGACAGGAATATGCAGAGCTTCTCGAAGAATTCATGACTGCAGTTAAGCAGAACTACGGAGAGAAAGTCCTAGTTCAGTTCGAAGATTTTGCAAACCACAATGCATTTGAACTGCTGTCTAAATACAGCAAGACTCACCTTGTCTTCAATGATGACATTCAGGGAACAGCATCTGTGGTCTTAGCAGGGCTCATCGCTTCCTTGAAATTACTTGGCGGAACATTAGGTGACCATACTTTCTTATTTCTTGGTGCTGGAGAGGCTGGAACCGGTATAGCAGAGCTTATAGCTCTTGAGATATCAAAAAAGACCGGCGCTCCATTGGAAGAAGCCCGCAAGAAGATTTGGCTTGTGGATTCTAAGGGATTGATTGTTAAATCTCGCCTAGGATCACTTCAACACTTTAAGCAGCCTTGGGCTCATGATCATGAACCCATAAAGCAACTTGTAGATGCCGTGAAGGCAATCAAGCCAACAGTGCTGATCGGAACATCTGGTGTGGGAAAACAATTCACGAAGGACGTTGTTGAGACCATGGCATCCTTGAATGAGAAACCACTTATCCTTGCTCTTTCCAACCCAACATCGCAAGCTGAGTGTACTGCTGAAGAAGCTTACACATGGACCAAGGGTCGAGCAATTTTCGGTAGTGGAAGCCCATTTGACCCTGTCGAATACGAGAACAAACTCCTAGTGCCTGGCCAGGCAAACAATGCCTACATATTCCCCGGTTTTGGCTTGGGATTGATCATGGCTGGTGCCATTCGCGTACACGATGACATGCTTCTGGCAGCCTCTGAAGCTCTGGCTATGCAAGTTTCCGAGGAACACTACGCCAAAGGATTGATCTACCCACCATTTACCAACATCAGAAAGATATCAGCCAACATTGCTGCTAAAGTCGCTGCTAAGGTCTATGAACTCGGTCTGGCTTCTAATCTGCCTCGACCCAAGGATCTCGTCAAGTATGCCGAGAGTTGCATGTACAGCCCGCGCTACAGAAGCTACCGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004470 GO:0004473 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0005911 GO:0006081 GO:0006082 GO:0006090 GO:0006098 GO:0006108 GO:0006139 GO:0006725 GO:0006732 GO:0006733 GO:0006739 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009051 GO:0009117 GO:0009506 GO:0009507 GO:0009536 GO:0009987 GO:0010035 GO:0010038 GO:0016020 GO:0016043 GO:0016491 GO:0016614 GO:0016615 GO:0016616 GO:0019362 GO:0019637 GO:0019682 GO:0019693 GO:0019752 GO:0022607 GO:0030054 GO:0030312 GO:0031090 GO:0032787 GO:0034641 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043648 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044281 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046496 GO:0046686 GO:0048046 GO:0050896 GO:0051156 GO:0051186 GO:0051259 GO:0051260 GO:0055044 GO:0055086 GO:0055114 GO:0065003 GO:0071704 GO:0071840 GO:0071944 GO:0072524 GO:0098588 GO:0098805 GO:1901135 GO:1901360 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00620 ko00710 ko01100 ko01120 ko01200 map00620 map00710 map01100 map01120 map01200

Pfam Domains

Protein Families

Malic_M malic

Protein Analysis

594

Amino Acids

65.09

Weight (kDa)

5.89

Isoelectric Point (pI)

35.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
malic	PF00390	118 - 298	1.8e-77	Malic enzyme, N-terminal domain
Malic_M	PF03949	309 - 561	2.1e-95	Malic enzyme, NAD binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007010)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G11670 AT5G25880 AT5G25880 AT5G25880
fragaria_vesca	FvH4_2g35330 FvH4_2g35330 FvH4_2g35330
malus_domestica	MD08G1111500.v1.1 MD15G1091000.v1.1
prunus_persica	Prupe.1G445400_v2.0.a1 Prupe.1G445400_v2.0.a1 Prupe.1G445400_v2.0.a1 Prupe.1G445400_v2.0.a1 Prupe.1G445400_v2.0.a1
pyrus_communis	pycom08g09260 pycom15g08500
rosa_chinensis	RchiOBHm_Chr6g0306591
rosa_laevigata	RLG00000010782
rosa_multiflora	Rmu_sc0017624.1_g000001
rosa_roxburghii	Rroxscaffold_7G00161630
rosa_rugosa	Rorug06G0350700 Rorug06G0350800
rosa_samantha	Rh6AG463100 Rh6BG437800
rosa_wichuraiana	Rw6G040350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	378
Acc16I	TGCGCA	1 cut(s)	228
AccB1I	GGYRCC	1 cut(s)	1526
AccB7I	CCANNNNNTGG	3 cut(s)	46, 434, 616
AccI	GTMKAC	1 cut(s)	647
AccII	CGCG	3 cut(s)	240, 1536, 1762
AciI	CCGC	5 cut(s)	182, 240, 984, 1102, 1760
AclWI	GGATC	6 cut(s)	173, 354, 508, 606, 1165, 1731
AcoI	YGGCCR	3 cut(s)	37, 536, 1468
AcsI	RAATTY	1 cut(s)	797
AcuI	CTGAAG	5 cut(s)	260, 335, 657, 1395, 1587
AdeI	CACNNNGTG	1 cut(s)	46
AfaI	GTAC	7 cut(s)	98, 332, 422, 525, 1369, 1539, 1754
AfiI	CCNNNNNNNGG	6 cut(s)	46, 268, 434, 545, 616, 1502
AflIII	ACRYGT	1 cut(s)	93
AgeI	ACCGGT	1 cut(s)	1037
AgsI	TTSAA	8 cut(s)	499, 521, 554, 878, 916, 973, 1166, 1315
AhdI	GACNNNNNGTC	1 cut(s)	1398
AjiI	CACGTC	1 cut(s)	94
AjnI	CCWGG	3 cut(s)	609, 1465, 1470
AjuI	GAANNNNNNNTTGG	4 cut(s)	51, 83, 300, 332
Alw26I	GTCTC	1 cut(s)	1292
AlwI	GGATC	6 cut(s)	173, 354, 508, 606, 1165, 1731
AlwNI	CAGNNNCTG	2 cut(s)	941, 1565
Ama87I	CYCGRG	1 cut(s)	593
AoxI	GGCC	4 cut(s)	37, 241, 536, 1468
ApeKI	GCWGC	7 cut(s)	172, 406, 606, 1177, 1559, 1661, 1673
ApoI	RAATTY	1 cut(s)	797
AsiGI	ACCGGT	1 cut(s)	1037
AspA2I	CCTAGG	1 cut(s)	1153
AspLEI	GCGC	4 cut(s)	229, 240, 1085, 1764
AspS9I	GGNCC	1 cut(s)	1392
AsuC2I	CCSGG	1 cut(s)	1497
AsuHPI	GGTGA	5 cut(s)	113, 449, 587, 896, 1007
AsuII	TTCGAA	1 cut(s)	849
AvaI	CYCGRG	1 cut(s)	593
AvaII	GGWCC	1 cut(s)	1392
AvrII	CCTAGG	1 cut(s)	1153
BalI	TGGCCA	3 cut(s)	39, 538, 1470
BanI	GGYRCC	1 cut(s)	1526
BanII	GRGCYC	3 cut(s)	594, 960, 1189
BbsI	GAAGAC	1 cut(s)	904
BbvI	GCAGC	7 cut(s)	159, 393, 593, 1189, 1571, 1648, 1660
BccI	CCATC	3 cut(s)	337, 566, 701
BceAI	ACGGC	2 cut(s)	242, 1208
BcgI	CGANNNNNNTGC	4 cut(s)	284, 318, 839, 873
BciT130I	CCWGG	3 cut(s)	611, 1467, 1472
BciVI	GTATCC	1 cut(s)	155
BclI	TGATCA	2 cut(s)	1192, 1515
BcnI	CCSGG	1 cut(s)	1497
BcoDI	GTCTC	1 cut(s)	1292
BfaI	CTAG	4 cut(s)	156, 839, 1154, 1460
BfmI	CTRYAG	2 cut(s)	807, 1765
BfoI	RGCGCY	1 cut(s)	1086
BfuI	GTATCC	1 cut(s)	155
BglI	GCCNNNNNGGC	1 cut(s)	1229
BisI	GCNGC	8 cut(s)	173, 241, 407, 607, 1178, 1560, 1662, 1674
BlnI	CCTAGG	1 cut(s)	1153
BlsI	GCNGC	8 cut(s)	174, 242, 408, 608, 1179, 1561, 1663, 1675
BmcAI	AGTACT	1 cut(s)	525
Bme1390I	CCNGG	4 cut(s)	611, 1467, 1472, 1497
Bme18I	GGWCC	1 cut(s)	1392
BmeRI	GACNNNNNGTC	1 cut(s)	1398
BmeT110I	CYCGRG	1 cut(s)	593
BmgBI	CACGTC	1 cut(s)	94
BmgT120I	GGNCC	1 cut(s)	1392
BmiI	GGNNCC	2 cut(s)	1036, 1528
BmrFI	CCNGG	4 cut(s)	611, 1467, 1472, 1497
BmsI	GCATC	6 cut(s)	214, 282, 481, 947, 1210, 1316
BpiI	GAAGAC	1 cut(s)	904
BpmI	CTGGAG	2 cut(s)	237, 1044
Bpu10I	CCTNAGC	1 cut(s)	1677
Bpu14I	TTCGAA	1 cut(s)	849
BpuEI	CTTGAG	1 cut(s)	1082
BpuMI	CCSGG	1 cut(s)	1497
Bsa29I	ATCGAT	1 cut(s)	696
BsaI	GGTCTC	1 cut(s)	1292
BsaWI	WCCGGW	1 cut(s)	1037
BsaXI	ACNNNNNCTCC	6 cut(s)	272, 302, 819, 849, 1069, 1099
Bsc4I	CCNNNNNNNGG	6 cut(s)	46, 268, 434, 545, 616, 1502
Bse118I	RCCGGY	2 cut(s)	1037, 1079
Bse1I	ACTGG	2 cut(s)	539, 772
Bse3DI	GCAATG	4 cut(s)	167, 333, 345, 1656
BseBI	CCWGG	3 cut(s)	611, 1467, 1472
BseCI	ATCGAT	1 cut(s)	696
BseGI	GGATG	3 cut(s)	58, 472, 1307
BseLI	CCNNNNNNNGG	6 cut(s)	46, 268, 434, 545, 616, 1502
BseMI	GCAATG	4 cut(s)	167, 333, 345, 1656
BseMII	CTCAG	3 cut(s)	57, 281, 1353
BseNI	ACTGG	2 cut(s)	539, 772
BseRI	GAGGAG	3 cut(s)	32, 416, 675
BseXI	GCAGC	7 cut(s)	159, 393, 593, 1189, 1571, 1648, 1660
BsgI	GTGCAG	1 cut(s)	636
Bsh1236I	CGCG	3 cut(s)	240, 1536, 1762
BshFI	GGCC	4 cut(s)	39, 243, 538, 1470
BshNI	GGYRCC	1 cut(s)	1526
BshTI	ACCGGT	1 cut(s)	1037
BshVI	ATCGAT	1 cut(s)	696
BsiHKCI	CYCGRG	1 cut(s)	593
BsiSI	CCGG	3 cut(s)	1038, 1080, 1497
BslI	CCNNNNNNNGG	6 cut(s)	46, 268, 434, 545, 616, 1502
BsmAI	GTCTC	1 cut(s)	1292
BsnI	GGCC	4 cut(s)	39, 243, 538, 1470
Bso31I	GGTCTC	1 cut(s)	1292
BsoBI	CYCGRG	1 cut(s)	593
Bsp119I	TTCGAA	1 cut(s)	849
Bsp1286I	GDGCHC	3 cut(s)	594, 960, 1189
Bsp1407I	TGTACA	2 cut(s)	420, 1752
Bsp19I	CCATGG	2 cut(s)	137, 1302
BspACI	CCGC	5 cut(s)	182, 240, 984, 1102, 1760
BspANI	GGCC	4 cut(s)	39, 243, 538, 1470
BspCNI	CTCAG	3 cut(s)	56, 280, 1354
BspDI	ATCGAT	1 cut(s)	696
BspFNI	CGCG	3 cut(s)	240, 1536, 1762
BspHI	TCATGA	3 cut(s)	801, 1189, 1195
BspLI	GGNNCC	2 cut(s)	1036, 1528
BspMAI	CTGCAG	1 cut(s)	811
BspPI	GGATC	6 cut(s)	173, 354, 508, 606, 1165, 1731
BspT104I	TTCGAA	1 cut(s)	849
BspT107I	GGYRCC	1 cut(s)	1526
BspTNI	GGTCTC	1 cut(s)	1292
BsrDI	GCAATG	4 cut(s)	167, 333, 345, 1656
BsrFI	RCCGGY	2 cut(s)	1037, 1079
BsrGI	TGTACA	2 cut(s)	420, 1752
BsrI	ACTGG	2 cut(s)	539, 772
BssAI	RCCGGY	2 cut(s)	1037, 1079
BssNAI	GTATAC	1 cut(s)	648
BssT1I	CCWWGG	6 cut(s)	137, 1153, 1181, 1302, 1395, 1719
Bst1107I	GTATAC	1 cut(s)	648
Bst2UI	CCWGG	3 cut(s)	611, 1467, 1472
Bst4CI	ACNGT	6 cut(s)	32, 101, 372, 433, 1246, 1778
BstAPI	GCANNNNNTGC	2 cut(s)	235, 1481
BstAUI	TGTACA	2 cut(s)	420, 1752
BstBI	TTCGAA	1 cut(s)	849
BstC8I	GCNNGC	6 cut(s)	445, 679, 683, 1102, 1359, 1760
BstDEI	CTNAG	6 cut(s)	43, 267, 949, 1128, 1362, 1677
BstDSI	CCRYGG	3 cut(s)	114, 137, 1302
BstEII	GGTNACC	1 cut(s)	995
BstENI	CCTNNNNNAGG	1 cut(s)	266
BstF5I	GGATG	3 cut(s)	58, 472, 1307
BstFNI	CGCG	3 cut(s)	240, 1536, 1762
BstH2I	RGCGCY	1 cut(s)	1086
BstHHI	GCGC	4 cut(s)	229, 240, 1085, 1764
BstMAI	GTCTC	1 cut(s)	1292
BstNI	CCWGG	3 cut(s)	611, 1467, 1472
BstNSI	RCATGY	2 cut(s)	1552, 1753
BstPI	GGTNACC	1 cut(s)	995
BstSCI	CCNGG	4 cut(s)	609, 1465, 1470, 1495
BstSFI	CTRYAG	2 cut(s)	807, 1765
BstUI	CGCG	3 cut(s)	240, 1536, 1762
BstV1I	GCAGC	7 cut(s)	159, 393, 593, 1189, 1571, 1648, 1660
BstV2I	GAAGAC	1 cut(s)	904
BstX2I	RGATCY	4 cut(s)	346, 513, 598, 1723
BstXI	CCANNNNNNTGG	1 cut(s)	455
BstYI	RGATCY	4 cut(s)	346, 513, 598, 1723
BstZ17I	GTATAC	1 cut(s)	648
Bsu15I	ATCGAT	1 cut(s)	696
BsuI	GTATCC	1 cut(s)	155
BsuRI	GGCC	4 cut(s)	39, 243, 538, 1470
BsuTUI	ATCGAT	1 cut(s)	696
BtgI	CCRYGG	3 cut(s)	114, 137, 1302
BtgZI	GCGATG	2 cut(s)	946, 1338
BtrI	CACGTC	1 cut(s)	94
BtsCI	GGATG	3 cut(s)	58, 472, 1307
BtsIMutI	CAGTG	2 cut(s)	51, 1251
Cac8I	GCNNGC	6 cut(s)	445, 679, 683, 1102, 1359, 1760
CaiI	CAGNNNCTG	2 cut(s)	941, 1565
CciI	TCATGA	3 cut(s)	801, 1189, 1195
CfoI	GCGC	4 cut(s)	229, 240, 1085, 1764
Cfr10I	RCCGGY	2 cut(s)	1037, 1079
Cfr13I	GGNCC	1 cut(s)	1392
ClaI	ATCGAT	1 cut(s)	696
Csp6I	GTAC	7 cut(s)	97, 331, 421, 524, 1368, 1538, 1753
CspAI	ACCGGT	1 cut(s)	1037
CviQI	GTAC	7 cut(s)	97, 331, 421, 524, 1368, 1538, 1753
DdeI	CTNAG	6 cut(s)	43, 267, 949, 1128, 1362, 1677
DraIII	CACNNNGTG	1 cut(s)	46
DriI	GACNNNNNGTC	1 cut(s)	1398
EaeI	YGGCCR	3 cut(s)	37, 536, 1468
Eam1105I	GACNNNNNGTC	1 cut(s)	1398
EciI	GGCGGA	1 cut(s)	999
Eco130I	CCWWGG	6 cut(s)	137, 1153, 1181, 1302, 1395, 1719
Eco24I	GRGCYC	3 cut(s)	594, 960, 1189
Eco31I	GGTCTC	1 cut(s)	1292
Eco32I	GATATC	2 cut(s)	1068, 1647
Eco47I	GGWCC	1 cut(s)	1392
Eco57I	CTGAAG	5 cut(s)	260, 335, 657, 1395, 1587
Eco88I	CYCGRG	1 cut(s)	593
Eco91I	GGTNACC	1 cut(s)	995
EcoNI	CCTNNNNNAGG	1 cut(s)	266
EcoO65I	GGTNACC	1 cut(s)	995
EcoRI	GAATTC	1 cut(s)	797
EcoRII	CCWGG	3 cut(s)	609, 1465, 1470
EcoRV	GATATC	2 cut(s)	1068, 1647
EcoT14I	CCWWGG	6 cut(s)	137, 1153, 1181, 1302, 1395, 1719
EcoT22I	ATGCAT	1 cut(s)	874
EcoT38I	GRGCYC	3 cut(s)	594, 960, 1189
ErhI	CCWWGG	6 cut(s)	137, 1153, 1181, 1302, 1395, 1719
FalI	AAGNNNNNCTT	2 cut(s)	56, 88
FauI	CCCGC	2 cut(s)	1109, 1767
FbaI	TGATCA	2 cut(s)	1192, 1515
FblI	GTMKAC	1 cut(s)	647
Fnu4HI	GCNGC	8 cut(s)	173, 241, 407, 607, 1178, 1560, 1662, 1674
FokI	GGATG	3 cut(s)	65, 459, 1294
FriOI	GRGCYC	3 cut(s)	594, 960, 1189
Fsp4HI	GCNGC	8 cut(s)	173, 241, 407, 607, 1178, 1560, 1662, 1674
FspAI	RTGCGCAY	1 cut(s)	228
FspBI	CTAG	4 cut(s)	156, 839, 1154, 1460
FspI	TGCGCA	1 cut(s)	228
GlaI	GCGC	4 cut(s)	228, 239, 1084, 1763
GluI	GCNGC	8 cut(s)	173, 241, 407, 607, 1178, 1560, 1662, 1674
GsuI	CTGGAG	2 cut(s)	237, 1044
HaeII	RGCGCY	1 cut(s)	1086
HaeIII	GGCC	4 cut(s)	39, 243, 538, 1470
HapII	CCGG	3 cut(s)	1038, 1080, 1497
HhaI	GCGC	4 cut(s)	229, 240, 1085, 1764
Hin6I	GCGC	4 cut(s)	227, 238, 1083, 1762
HinP1I	GCGC	4 cut(s)	227, 238, 1083, 1762
HindIII	AAGCTT	2 cut(s)	380, 1380
HinfI	GANTC	4 cut(s)	265, 622, 901, 1124
HpaII	CCGG	3 cut(s)	1038, 1080, 1497
HphI	GGTGA	5 cut(s)	113, 449, 587, 896, 1007
Hpy166II	GTNNAC	6 cut(s)	97, 421, 423, 648, 1368, 1540
Hpy188I	TCNGA	5 cut(s)	659, 1256, 1567, 1591, 1640
Hpy8I	GTNNAC	6 cut(s)	97, 421, 423, 648, 1368, 1540
HpyAV	CCTTC	8 cut(s)	208, 214, 254, 359, 499, 681, 1222, 1279
HpyCH4III	ACNGT	6 cut(s)	32, 101, 372, 433, 1246, 1778
HpyCH4IV	ACGT	2 cut(s)	93, 1290
HpyF3I	CTNAG	6 cut(s)	43, 267, 949, 1128, 1362, 1677
HpySE526I	ACGT	2 cut(s)	93, 1290
HspAI	GCGC	4 cut(s)	227, 238, 1083, 1762
Ksp22I	TGATCA	2 cut(s)	1192, 1515
LmnI	GCTCC	5 cut(s)	6, 271, 403, 459, 1090
Lsp1109I	GCAGC	7 cut(s)	159, 393, 593, 1189, 1571, 1648, 1660
LweI	GCATC	6 cut(s)	214, 282, 481, 947, 1210, 1316
MaeI	CTAG	4 cut(s)	156, 839, 1154, 1460
MaeII	ACGT	2 cut(s)	93, 1290
MaeIII	GTNAC	2 cut(s)	565, 995
MflI	RGATCY	4 cut(s)	346, 513, 598, 1723
MhlI	GDGCHC	3 cut(s)	594, 960, 1189
MlsI	TGGCCA	3 cut(s)	39, 538, 1470
MluCI	AATT	4 cut(s)	797, 974, 1277, 1407
MluNI	TGGCCA	3 cut(s)	39, 538, 1470
MlyI	GAGTC	2 cut(s)	259, 895
MmeI	TCCRAC	2 cut(s)	610, 1368
Mox20I	TGGCCA	3 cut(s)	39, 538, 1470
Mph1103I	ATGCAT	1 cut(s)	874
MscI	TGGCCA	3 cut(s)	39, 538, 1470
MseI	TTAA	3 cut(s)	813, 1143, 1173
MslI	CAYNNNNRTG	2 cut(s)	338, 1194
Msp20I	TGGCCA	3 cut(s)	39, 538, 1470
MspI	CCGG	3 cut(s)	1038, 1080, 1497
MspR9I	CCNGG	4 cut(s)	611, 1467, 1472, 1497
MvaI	CCWGG	3 cut(s)	611, 1467, 1472
MvnI	CGCG	3 cut(s)	240, 1536, 1762
NciI	CCSGG	1 cut(s)	1497
NcoI	CCATGG	2 cut(s)	137, 1302
NlaIV	GGNNCC	2 cut(s)	1036, 1528
NmeAIII	GCCGAG	1 cut(s)	1765
NmuCI	GTSAC	2 cut(s)	565, 995
NsbI	TGCGCA	1 cut(s)	228
NsiI	ATGCAT	1 cut(s)	874
NspI	RCATGY	2 cut(s)	1552, 1753
NspV	TTCGAA	1 cut(s)	849
PagI	TCATGA	3 cut(s)	801, 1189, 1195
PfeI	GAWTC	2 cut(s)	622, 1124
PflFI	GACNNNGTC	1 cut(s)	1436
PflMI	CCANNNNNTGG	3 cut(s)	46, 434, 616
PinAI	ACCGGT	1 cut(s)	1037
PkrI	GCNGC	8 cut(s)	174, 242, 408, 608, 1179, 1561, 1663, 1675
PleI	GAGTC	2 cut(s)	259, 895
PpsI	GAGTC	2 cut(s)	259, 895
PsiI	TTATAA	1 cut(s)	378
Psp6I	CCWGG	3 cut(s)	609, 1465, 1470
PspEI	GGTNACC	1 cut(s)	995
PspGI	CCWGG	3 cut(s)	609, 1465, 1470
PspN4I	GGNNCC	2 cut(s)	1036, 1528
PspPI	GGNCC	1 cut(s)	1392
PstI	CTGCAG	1 cut(s)	811
PstNI	CAGNNNCTG	2 cut(s)	941, 1565
PsuI	RGATCY	4 cut(s)	346, 513, 598, 1723
PsyI	GACNNNGTC	1 cut(s)	1436
RsaI	GTAC	7 cut(s)	98, 332, 422, 525, 1369, 1539, 1754
RsaNI	GTAC	7 cut(s)	97, 331, 421, 524, 1368, 1538, 1753
RseI	CAYNNNNRTG	2 cut(s)	338, 1194
SaqAI	TTAA	3 cut(s)	813, 1143, 1173
SatI	GCNGC	8 cut(s)	173, 241, 407, 607, 1178, 1560, 1662, 1674
Sau96I	GGNCC	1 cut(s)	1392
ScaI	AGTACT	1 cut(s)	525
SchI	GAGTC	2 cut(s)	259, 895
ScrFI	CCNGG	4 cut(s)	611, 1467, 1472, 1497
SduI	GDGCHC	3 cut(s)	594, 960, 1189
SfaNI	GCATC	6 cut(s)	214, 282, 481, 947, 1210, 1316
SfcI	CTRYAG	2 cut(s)	807, 1765
SfuI	TTCGAA	1 cut(s)	849
SinI	GGWCC	1 cut(s)	1392
SmiMI	CAYNNNNRTG	2 cut(s)	338, 1194
SmlI	CTYRAG	1 cut(s)	1061
SmoI	CTYRAG	1 cut(s)	1061
Sse9I	AATT	4 cut(s)	797, 974, 1277, 1407
SsiI	CCGC	5 cut(s)	182, 240, 984, 1102, 1760
SspMI	CTAG	4 cut(s)	156, 839, 1154, 1460
StyD4I	CCNGG	4 cut(s)	609, 1465, 1470, 1495
StyI	CCWWGG	6 cut(s)	137, 1153, 1181, 1302, 1395, 1719
TaaI	ACNGT	6 cut(s)	32, 101, 372, 433, 1246, 1778
TaiI	ACGT	2 cut(s)	96, 1293
TaqI	TCGA	6 cut(s)	696, 792, 849, 1402, 1440, 1714
TaqII	GACCGA	1 cut(s)	1682
TasI	AATT	4 cut(s)	797, 974, 1277, 1407
TatI	WGTACW	4 cut(s)	420, 523, 1367, 1752
TauI	GCSGC	1 cut(s)	243
TfiI	GAWTC	2 cut(s)	622, 1124
Tru1I	TTAA	3 cut(s)	813, 1143, 1173
Tru9I	TTAA	3 cut(s)	813, 1143, 1173
TscAI	CASTG	2 cut(s)	51, 1251
TseFI	GTSAC	2 cut(s)	565, 995
TseI	GCWGC	7 cut(s)	172, 406, 606, 1177, 1559, 1661, 1673
Tsp45I	GTSAC	2 cut(s)	565, 995
TspDTI	ATGAA	5 cut(s)	327, 378, 790, 1212, 1701
TspGWI	ACGGA	3 cut(s)	131, 309, 840
TspRI	CASTG	2 cut(s)	51, 1251
Tth111I	GACNNNGTC	1 cut(s)	1436
Van91I	CCANNNNNTGG	3 cut(s)	46, 434, 616
VpaK11BI	GGWCC	1 cut(s)	1392
XagI	CCTNNNNNAGG	1 cut(s)	266
XapI	RAATTY	1 cut(s)	797
XceI	RCATGY	2 cut(s)	1552, 1753
XmaJI	CCTAGG	1 cut(s)	1153
XmiI	GTMKAC	1 cut(s)	647
XspI	CTAG	4 cut(s)	156, 839, 1154, 1460
ZrmI	AGTACT	1 cut(s)	525
Zsp2I	ATGCAT	1 cut(s)	874

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.