Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0007010

Physical Location & Seq

Genomic Coordinates Forward (+)

64177553 .. 64182571

Gene Structure 5019 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G040350.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1746 bp

ATGGGGAGTATAGTGAAGGAGATGGAGAGCAAGGCTGGTTTTGGTGGGGGTGTTCAGGACGTGTACGGTGAGGATTGTGCTTCGGAGGATCAGGTTCTCACCCCCTGGACTGCTTCTGTTGCAAGTGGACATACTTTGATGCGGGAACCACGCTACAACAAAGGACTTGCCTTCACTGAGAGGGAGAGAGATGCTCATTACTTGCGTGGGCTTCTGCCTCCAGCCGTCCTGACCCAAGAGCTTCAGGAAAAGAAGATGATGCACAATCTTCGAAAATATGAGGTTCTATTGCAAAGATATATGGCCATGATGGATCTTCAGGAGAGAAATGAAGGGCTGTTTTATAAGCTTTTGATTGATAATGTTGAGTTGCTCCCTGTCGTGTACACCCCTACTGTTGGTGAGGCTTGCCAAAAATATGGTAGCATTTTCAGGCGTCCTCAGGGTCTTTATGTCAGTTTAAAAGACAAGGGAAAAATCCTTGAAGTATTAAAAAACTGGCCAGAAAGGAGAATTCAAGTTATTGTTGTGACTGATGGTGAGCGTATTTTGGGGCTTGGAGATCTTGGCTGTCAGGGAATGGGAATTCCAGTTGGAAAGCTTTCTTTGTATACAGCATTGGGAGGAGTTCCTCCTTCAGCATGCTTGCCTATAACCATTGATGTTGGCACAAACAATGAGAAGTTGCTGAATGATGAGTTTTACACTGGGCTTAAGCACAAGAGGGCAACTGGACAGGAATATGCAGAGCTTCTTGAGGAATTCATGACTGCGGTCAAGAGGAATTATGGGGAGAAAATTCTAGTTCAGTTTGAAGATTTTGCAAACCACAATGCATTTGAACTGCTGTCTAAATACAGTTCAAGTCACCTTGTGTTTAATGATGATATACAGGGAACAGCATCTGTGGTGTTAGCAGGGCTTATAGCATCCTTGAAATTAATTGGGGGAACTTTAGCTGATCATACTTTCTTATTTTTGGGTGCCGGAGAGGCTGGAACTGGTATAGCGGAGCTTATTGCTCTTGAGATATCAAAAAAGACCAAAGCTCCTGTTGAAGAAACCCGTAAGAAGATTTGGCTTGTGGATTCAAAGGGATTGATTGTTCAATCTCGTGTAGAATCACTTCAACATTTTAAGAAGCCTTGGGCTCATGAGCATGAACCCGTAAGGGAACTTGTCGATGCTGTGAAGGCAATCAAACCAACAGTGTTGATAGGAACATCTGGTGTAGGAAAGCAATTTACACAGGAAGTGGTCGAGGCCATGGCATCTTTTAATGAGAAACCTCTTATCCTCGCTCTTTCCAACCCAACATCACAATCTGAGTGTACTGCAGAAGAAGCTTACACATGGACCAAGGGTCAAGCAATCTTTGCTAGTGGAAGCCCATTTGACCCGGTTGAATATGAGGACAAACTCCTAGTGCCTGGCCAGGCCAACAATGCTTACATATTTCCTGGTTTCGGTTTGGGATTGATCATCTCTGGTGCCATTCGTGTACACGATGAAATGCTTTTGGCAGCCTCTGAAGCTTTGGCTACCCAAGTCACCCAAGAAAACTACGACAAAGGATTGATTTACCCACCATTTACCAACATCAGAAAGATATCAGCAAACATCGCTGCTAAGGTCGCTGCTACGGTTTATGACCTTGGTCTGGCTTCTAATCTGCCTCGACCAAAAGATCTTGTTAAGTATGCAGAGAGCAGAATGTACAGCCCACTCTACAGAAACTATCTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004470 GO:0004473 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0005911 GO:0006081 GO:0006082 GO:0006090 GO:0006098 GO:0006108 GO:0006139 GO:0006725 GO:0006732 GO:0006733 GO:0006739 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009051 GO:0009117 GO:0009506 GO:0009507 GO:0009536 GO:0009987 GO:0010035 GO:0010038 GO:0016020 GO:0016043 GO:0016491 GO:0016614 GO:0016615 GO:0016616 GO:0019362 GO:0019637 GO:0019682 GO:0019693 GO:0019752 GO:0022607 GO:0030054 GO:0030312 GO:0031090 GO:0032787 GO:0034641 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043648 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044281 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046496 GO:0046686 GO:0048046 GO:0050896 GO:0051156 GO:0051186 GO:0051259 GO:0051260 GO:0055044 GO:0055086 GO:0055114 GO:0065003 GO:0071704 GO:0071840 GO:0071944 GO:0072524 GO:0098588 GO:0098805 GO:1901135 GO:1901360 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00620 ko00710 ko01100 ko01120 ko01200 map00620 map00710 map01100 map01120 map01200

Pfam Domains

Protein Families

Malic_M malic

Protein Analysis

581

Amino Acids

64.19

Weight (kDa)

5.89

Isoelectric Point (pI)

38.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
malic	PF00390	107 - 286	4.2e-76	Malic enzyme, N-terminal domain
Malic_M	PF03949	297 - 548	6.8e-96	Malic enzyme, NAD binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0007010)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G11670 AT5G25880 AT5G25880 AT5G25880
fragaria_vesca	FvH4_2g35330 FvH4_2g35330 FvH4_2g35330
malus_domestica	MD08G1111500.v1.1 MD15G1091000.v1.1
prunus_persica	Prupe.1G445400_v2.0.a1 Prupe.1G445400_v2.0.a1 Prupe.1G445400_v2.0.a1 Prupe.1G445400_v2.0.a1 Prupe.1G445400_v2.0.a1
pyrus_communis	pycom08g09260 pycom15g08500
rosa_chinensis	RchiOBHm_Chr6g0306591
rosa_laevigata	RLG00000010782
rosa_multiflora	Rmu_sc0017624.1_g000001
rosa_roxburghii	Rroxscaffold_7G00161630
rosa_rugosa	Rorug06G0350700 Rorug06G0350800
rosa_samantha	Rh6AG463100 Rh6BG437800
rosa_wichuraiana	Rw6G040350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	345
AccB1I	GGYRCC	2 cut(s)	983, 1490
AccI	GTMKAC	1 cut(s)	611
AciI	CCGC	3 cut(s)	142, 773, 1010
AclWI	GGATC	2 cut(s)	96, 321
AcoI	YGGCCR	3 cut(s)	303, 500, 1432
AcsI	RAATTY	4 cut(s)	513, 585, 761, 798
AcuI	CTGAAG	4 cut(s)	227, 302, 621, 1551
AcyI	GRCGYC	1 cut(s)	436
AdeI	CACNNNGTG	1 cut(s)	874
AfaI	GTAC	5 cut(s)	65, 386, 1333, 1503, 1718
AfiI	CCNNNNNNNGG	3 cut(s)	398, 1466, 1660
AflII	CTTAAG	1 cut(s)	713
AflIII	ACRYGT	1 cut(s)	60
AhdI	GACNNNNNGTC	1 cut(s)	1362
AjiI	CACGTC	1 cut(s)	61
AjnI	CCWGG	4 cut(s)	104, 1429, 1434, 1459
AloI	GAACNNNNNNTCC	2 cut(s)	1089, 1121
AluBI	AGCT	9 cut(s)	241, 349, 601, 751, 959, 1015, 1049, 1346, 1535
AluI	AGCT	9 cut(s)	241, 349, 601, 751, 959, 1015, 1049, 1346, 1535
AlwI	GGATC	2 cut(s)	96, 321
AlwNI	CAGNNNCTG	2 cut(s)	905, 1529
AoxI	GGCC	5 cut(s)	303, 500, 1263, 1432, 1437
ApeKI	GCWGC	3 cut(s)	1523, 1625, 1637
ApoI	RAATTY	4 cut(s)	513, 585, 761, 798
AseI	ATTAAT	1 cut(s)	941
Asp700I	GAANNNNTTC	2 cut(s)	601, 1125
AspS9I	GGNCC	1 cut(s)	1356
AsuC2I	CCSGG	1 cut(s)	1400
AsuHPI	GGTGA	6 cut(s)	80, 91, 413, 551, 860, 1543
AsuII	TTCGAA	1 cut(s)	271
AvaII	GGWCC	1 cut(s)	1356
AxyI	CCTNAGG	1 cut(s)	441
BalI	TGGCCA	3 cut(s)	305, 502, 1434
BanI	GGYRCC	2 cut(s)	983, 1490
BanII	GRGCYC	1 cut(s)	1153
BarI	GAAGNNNNNNTAC	2 cut(s)	1110, 1142
BauI	CACGAG	1 cut(s)	1113
BbvI	GCAGC	3 cut(s)	1535, 1612, 1624
BccI	CCATC	3 cut(s)	16, 304, 530
BceAI	ACGGC	1 cut(s)	209
BcgI	CGANNNNNNTGC	2 cut(s)	251, 285
BciT130I	CCWGG	4 cut(s)	106, 1431, 1436, 1461
BclI	TGATCA	2 cut(s)	961, 1479
BcnI	CCSGG	1 cut(s)	1400
BfaI	CTAG	3 cut(s)	803, 1380, 1424
BfmI	CTRYAG	2 cut(s)	1335, 1729
BfrI	CTTAAG	1 cut(s)	713
BglI	GCCNNNNNGGC	1 cut(s)	992
BglII	AGATCT	2 cut(s)	562, 1687
BisI	GCNGC	3 cut(s)	1524, 1626, 1638
BlsI	GCNGC	3 cut(s)	1525, 1627, 1639
Bme1390I	CCNGG	5 cut(s)	106, 1400, 1431, 1436, 1461
Bme18I	GGWCC	1 cut(s)	1356
BmeRI	GACNNNNNGTC	1 cut(s)	1362
BmgBI	CACGTC	1 cut(s)	61
BmgT120I	GGNCC	1 cut(s)	1356
BmiI	GGNNCC	3 cut(s)	147, 985, 1492
BmrFI	CCNGG	5 cut(s)	106, 1400, 1431, 1436, 1461
BmrI	ACTGGG	1 cut(s)	717
BmsI	GCATC	7 cut(s)	129, 181, 249, 911, 938, 1174, 1280
BmuI	ACTGGG	1 cut(s)	717
BoxI	GACNNNNGTC	2 cut(s)	773, 1656
BplI	GAGNNNNNCTC	2 cut(s)	178, 210
BpmI	CTGGAG	1 cut(s)	204
Bpu10I	CCTNAGC	1 cut(s)	1629
Bpu14I	TTCGAA	1 cut(s)	271
BpuEI	CTTGAG	2 cut(s)	776, 1046
BpuMI	CCSGG	1 cut(s)	1400
BsaHI	GRCGYC	1 cut(s)	436
BsaJI	CCNNGG	5 cut(s)	104, 1145, 1266, 1359, 1654
BsaXI	ACNNNNNCTCC	2 cut(s)	1033, 1063
Bsc4I	CCNNNNNNNGG	3 cut(s)	398, 1466, 1660
Bse1I	ACTGG	5 cut(s)	503, 590, 712, 736, 1006
Bse21I	CCTNAGG	1 cut(s)	441
BseBI	CCWGG	4 cut(s)	106, 1431, 1436, 1461
BseDI	CCNNGG	5 cut(s)	104, 1145, 1266, 1359, 1654
BseGI	GGATG	1 cut(s)	929
BseLI	CCNNNNNNNGG	3 cut(s)	398, 1466, 1660
BseMII	CTCAG	3 cut(s)	168, 455, 1317
BseNI	ACTGG	5 cut(s)	503, 590, 712, 736, 1006
BseRI	GAGGAG	1 cut(s)	639
BseXI	GCAGC	3 cut(s)	1535, 1612, 1624
BshFI	GGCC	5 cut(s)	305, 502, 1265, 1434, 1439
BshNI	GGYRCC	2 cut(s)	983, 1490
BsiSI	CCGG	2 cut(s)	987, 1400
BslI	CCNNNNNNNGG	3 cut(s)	398, 1466, 1660
BsnI	GGCC	5 cut(s)	305, 502, 1265, 1434, 1439
Bsp119I	TTCGAA	1 cut(s)	271
Bsp1286I	GDGCHC	1 cut(s)	1153
Bsp1407I	TGTACA	3 cut(s)	384, 1501, 1716
Bsp143I	GATC	6 cut(s)	88, 313, 562, 961, 1479, 1687
Bsp19I	CCATGG	1 cut(s)	1266
BspACI	CCGC	3 cut(s)	142, 773, 1010
BspANI	GGCC	5 cut(s)	305, 502, 1265, 1434, 1439
BspCNI	CTCAG	3 cut(s)	169, 454, 1318
BspHI	TCATGA	2 cut(s)	765, 1153
BspLI	GGNNCC	3 cut(s)	147, 985, 1492
BspMAI	CTGCAG	1 cut(s)	1339
BspPI	GGATC	2 cut(s)	96, 321
BspT104I	TTCGAA	1 cut(s)	271
BspT107I	GGYRCC	2 cut(s)	983, 1490
BspTI	CTTAAG	1 cut(s)	713
BsrGI	TGTACA	3 cut(s)	384, 1501, 1716
BsrI	ACTGG	5 cut(s)	503, 590, 712, 736, 1006
BssECI	CCNNGG	5 cut(s)	104, 1145, 1266, 1359, 1654
BssMI	GATC	6 cut(s)	88, 313, 562, 961, 1479, 1687
BssNAI	GTATAC	1 cut(s)	612
BssNI	GRCGYC	1 cut(s)	436
BssSI	CACGAG	1 cut(s)	1113
BssT1I	CCWWGG	4 cut(s)	1145, 1266, 1359, 1654
Bst1107I	GTATAC	1 cut(s)	612
Bst2BI	CACGAG	1 cut(s)	1113
Bst2UI	CCWGG	4 cut(s)	106, 1431, 1436, 1461
Bst4CI	ACNGT	5 cut(s)	68, 397, 860, 1210, 1645
BstACI	GRCGYC	1 cut(s)	436
BstAFI	CTTAAG	1 cut(s)	713
BstAPI	GCANNNNNTGC	1 cut(s)	1376
BstAUI	TGTACA	3 cut(s)	384, 1501, 1716
BstBI	TTCGAA	1 cut(s)	271
BstC8I	GCNNGC	3 cut(s)	409, 643, 647
BstDEI	CTNAG	4 cut(s)	177, 441, 1326, 1629
BstDSI	CCRYGG	1 cut(s)	1266
BstF5I	GGATG	1 cut(s)	929
BstKTI	GATC	6 cut(s)	91, 316, 565, 964, 1482, 1690
BstMBI	GATC	6 cut(s)	88, 313, 562, 961, 1479, 1687
BstMWI	GCNNNNNNNGC	9 cut(s)	119, 992, 1193, 1343, 1376, 1445, 1532, 1622, 1634
BstNI	CCWGG	4 cut(s)	106, 1431, 1436, 1461
BstNSI	RCATGY	1 cut(s)	645
BstPAI	GACNNNNGTC	2 cut(s)	773, 1656
BstSCI	CCNGG	5 cut(s)	104, 1398, 1429, 1434, 1459
BstSFI	CTRYAG	2 cut(s)	1335, 1729
BstV1I	GCAGC	3 cut(s)	1535, 1612, 1624
BstX2I	RGATCY	3 cut(s)	313, 562, 1687
BstXI	CCANNNNNNTGG	1 cut(s)	419
BstYI	RGATCY	3 cut(s)	313, 562, 1687
BstZ17I	GTATAC	1 cut(s)	612
Bsu36I	CCTNAGG	1 cut(s)	441
BsuRI	GGCC	5 cut(s)	305, 502, 1265, 1434, 1439
BtgI	CCRYGG	1 cut(s)	1266
BtgZI	GCGATG	1 cut(s)	1606
BtrI	CACGTC	1 cut(s)	61
BtsCI	GGATG	1 cut(s)	929
BtsIMutI	CAGTG	3 cut(s)	174, 705, 1215
Cac8I	GCNNGC	3 cut(s)	409, 643, 647
CaiI	CAGNNNCTG	2 cut(s)	905, 1529
CciI	TCATGA	2 cut(s)	765, 1153
Cfr13I	GGNCC	1 cut(s)	1356
CseI	GACGC	1 cut(s)	425
Csp6I	GTAC	5 cut(s)	64, 385, 1332, 1502, 1717
CviAII	CATG	7 cut(s)	307, 642, 766, 1154, 1160, 1267, 1353
CviQI	GTAC	5 cut(s)	64, 385, 1332, 1502, 1717
DdeI	CTNAG	4 cut(s)	177, 441, 1326, 1629
DpnI	GATC	6 cut(s)	90, 315, 564, 963, 1481, 1689
DpnII	GATC	6 cut(s)	88, 313, 562, 961, 1479, 1687
DraI	TTTAAA	1 cut(s)	462
DraIII	CACNNNGTG	1 cut(s)	874
DriI	GACNNNNNGTC	1 cut(s)	1362
EaeI	YGGCCR	3 cut(s)	303, 500, 1432
Eam1105I	GACNNNNNGTC	1 cut(s)	1362
Eco130I	CCWWGG	4 cut(s)	1145, 1266, 1359, 1654
Eco24I	GRGCYC	1 cut(s)	1153
Eco32I	GATATC	2 cut(s)	1032, 1611
Eco47I	GGWCC	1 cut(s)	1356
Eco57I	CTGAAG	4 cut(s)	227, 302, 621, 1551
Eco81I	CCTNAGG	1 cut(s)	441
EcoRI	GAATTC	3 cut(s)	513, 585, 761
EcoRII	CCWGG	4 cut(s)	104, 1429, 1434, 1459
EcoRV	GATATC	2 cut(s)	1032, 1611
EcoT14I	CCWWGG	4 cut(s)	1145, 1266, 1359, 1654
EcoT22I	ATGCAT	1 cut(s)	838
EcoT38I	GRGCYC	1 cut(s)	1153
ErhI	CCWWGG	4 cut(s)	1145, 1266, 1359, 1654
FaeI	CATG	7 cut(s)	310, 645, 769, 1157, 1163, 1270, 1356
FatI	CATG	7 cut(s)	306, 641, 765, 1153, 1159, 1266, 1352
FauI	CCCGC	1 cut(s)	135
FbaI	TGATCA	2 cut(s)	961, 1479
FblI	GTMKAC	1 cut(s)	611
Fnu4HI	GCNGC	3 cut(s)	1524, 1626, 1638
FokI	GGATG	1 cut(s)	916
FriOI	GRGCYC	1 cut(s)	1153
Fsp4HI	GCNGC	3 cut(s)	1524, 1626, 1638
FspBI	CTAG	3 cut(s)	803, 1380, 1424
GluI	GCNGC	3 cut(s)	1524, 1626, 1638
GsuI	CTGGAG	1 cut(s)	204
HaeIII	GGCC	5 cut(s)	305, 502, 1265, 1434, 1439
HapII	CCGG	2 cut(s)	987, 1400
HgaI	GACGC	1 cut(s)	425
Hin1I	GRCGYC	1 cut(s)	436
Hin1II	CATG	7 cut(s)	310, 645, 769, 1157, 1163, 1270, 1356
HindIII	AAGCTT	4 cut(s)	347, 599, 1344, 1533
HinfI	GANTC	2 cut(s)	1088, 1121
HpaII	CCGG	2 cut(s)	987, 1400
HphI	GGTGA	6 cut(s)	80, 91, 413, 551, 860, 1543
Hpy166II	GTNNAC	8 cut(s)	64, 128, 385, 387, 612, 1332, 1502, 1504
Hpy188I	TCNGA	4 cut(s)	85, 1327, 1531, 1604
Hpy188III	TCNNGA	9 cut(s)	56, 229, 245, 320, 755, 766, 778, 1025, 1154
Hpy8I	GTNNAC	8 cut(s)	64, 128, 385, 387, 612, 1332, 1502, 1504
HpyAV	CCTTC	5 cut(s)	10, 181, 326, 645, 1186
HpyCH4III	ACNGT	5 cut(s)	68, 397, 860, 1210, 1645
HpyCH4IV	ACGT	1 cut(s)	60
HpyCH4V	TGCA	8 cut(s)	122, 262, 292, 746, 824, 836, 1337, 1703
HpyF10VI	GCNNNNNNNGC	9 cut(s)	119, 992, 1193, 1343, 1376, 1445, 1532, 1622, 1634
HpyF3I	CTNAG	4 cut(s)	177, 441, 1326, 1629
HpySE526I	ACGT	1 cut(s)	60
Hsp92I	GRCGYC	1 cut(s)	436
Hsp92II	CATG	7 cut(s)	310, 645, 769, 1157, 1163, 1270, 1356
Ksp22I	TGATCA	2 cut(s)	961, 1479
Kzo9I	GATC	6 cut(s)	88, 313, 562, 961, 1479, 1687
LmnI	GCTCC	3 cut(s)	378, 1012, 1054
Lsp1109I	GCAGC	3 cut(s)	1535, 1612, 1624
LweI	GCATC	7 cut(s)	129, 181, 249, 911, 938, 1174, 1280
MaeI	CTAG	3 cut(s)	803, 1380, 1424
MaeII	ACGT	1 cut(s)	60
MaeIII	GTNAC	3 cut(s)	529, 866, 1549
MalI	GATC	6 cut(s)	90, 315, 564, 963, 1481, 1689
MboI	GATC	6 cut(s)	88, 313, 562, 961, 1479, 1687
MboII	GAAGA	7 cut(s)	260, 265, 308, 827, 1070, 1084, 1352
MflI	RGATCY	3 cut(s)	313, 562, 1687
MhlI	GDGCHC	1 cut(s)	1153
MlsI	TGGCCA	3 cut(s)	305, 502, 1434
MluCI	AATT	8 cut(s)	513, 585, 761, 784, 798, 938, 942, 1241
MluNI	TGGCCA	3 cut(s)	305, 502, 1434
MmeI	TCCRAC	2 cut(s)	574, 1332
Mox20I	TGGCCA	3 cut(s)	305, 502, 1434
Mph1103I	ATGCAT	1 cut(s)	838
MroXI	GAANNNNTTC	2 cut(s)	601, 1125
MscI	TGGCCA	3 cut(s)	305, 502, 1434
MseI	TTAA	8 cut(s)	461, 491, 714, 879, 941, 1137, 1278, 1695
MslI	CAYNNNNRTG	1 cut(s)	1158
Msp20I	TGGCCA	3 cut(s)	305, 502, 1434
MspCI	CTTAAG	1 cut(s)	713
MspI	CCGG	2 cut(s)	987, 1400
MspR9I	CCNGG	5 cut(s)	106, 1400, 1431, 1436, 1461
MvaI	CCWGG	4 cut(s)	106, 1431, 1436, 1461
MwoI	GCNNNNNNNGC	9 cut(s)	119, 992, 1193, 1343, 1376, 1445, 1532, 1622, 1634
NciI	CCSGG	1 cut(s)	1400
NcoI	CCATGG	1 cut(s)	1266
NdeII	GATC	6 cut(s)	88, 313, 562, 961, 1479, 1687
NlaIII	CATG	7 cut(s)	310, 645, 769, 1157, 1163, 1270, 1356
NlaIV	GGNNCC	3 cut(s)	147, 985, 1492
NmuCI	GTSAC	3 cut(s)	529, 866, 1549
NsiI	ATGCAT	1 cut(s)	838
NspI	RCATGY	1 cut(s)	645
NspV	TTCGAA	1 cut(s)	271
PaeI	GCATGC	1 cut(s)	645
PagI	TCATGA	2 cut(s)	765, 1153
PdmI	GAANNNNTTC	2 cut(s)	601, 1125
PfeI	GAWTC	2 cut(s)	1088, 1121
PkrI	GCNGC	3 cut(s)	1525, 1627, 1639
PshAI	GACNNNNGTC	2 cut(s)	773, 1656
PshBI	ATTAAT	1 cut(s)	941
PsiI	TTATAA	1 cut(s)	345
Psp6I	CCWGG	4 cut(s)	104, 1429, 1434, 1459
PspGI	CCWGG	4 cut(s)	104, 1429, 1434, 1459
PspN4I	GGNNCC	3 cut(s)	147, 985, 1492
PspPI	GGNCC	1 cut(s)	1356
PstI	CTGCAG	1 cut(s)	1339
PstNI	CAGNNNCTG	2 cut(s)	905, 1529
PsuI	RGATCY	3 cut(s)	313, 562, 1687
RsaI	GTAC	5 cut(s)	65, 386, 1333, 1503, 1718
RsaNI	GTAC	5 cut(s)	64, 385, 1332, 1502, 1717
RseI	CAYNNNNRTG	1 cut(s)	1158
SaqAI	TTAA	8 cut(s)	461, 491, 714, 879, 941, 1137, 1278, 1695
SatI	GCNGC	3 cut(s)	1524, 1626, 1638
Sau3AI	GATC	6 cut(s)	88, 313, 562, 961, 1479, 1687
Sau96I	GGNCC	1 cut(s)	1356
ScrFI	CCNGG	5 cut(s)	106, 1400, 1431, 1436, 1461
SduI	GDGCHC	1 cut(s)	1153
SfaNI	GCATC	7 cut(s)	129, 181, 249, 911, 938, 1174, 1280
SfcI	CTRYAG	2 cut(s)	1335, 1729
SfuI	TTCGAA	1 cut(s)	271
SinI	GGWCC	1 cut(s)	1356
SmiMI	CAYNNNNRTG	1 cut(s)	1158
SmlI	CTYRAG	3 cut(s)	713, 755, 1025
SmoI	CTYRAG	3 cut(s)	713, 755, 1025
SphI	GCATGC	1 cut(s)	645
Sse9I	AATT	8 cut(s)	513, 585, 761, 784, 798, 938, 942, 1241
SsiI	CCGC	3 cut(s)	142, 773, 1010
SspMI	CTAG	3 cut(s)	803, 1380, 1424
StyD4I	CCNGG	5 cut(s)	104, 1398, 1429, 1434, 1459
StyI	CCWWGG	4 cut(s)	1145, 1266, 1359, 1654
TaaI	ACNGT	5 cut(s)	68, 397, 860, 1210, 1645
TaiI	ACGT	1 cut(s)	63
TaqI	TCGA	4 cut(s)	271, 1182, 1260, 1678
TasI	AATT	8 cut(s)	513, 585, 761, 784, 798, 938, 942, 1241
TatI	WGTACW	4 cut(s)	384, 1331, 1501, 1716
TfiI	GAWTC	2 cut(s)	1088, 1121
Tru1I	TTAA	8 cut(s)	461, 491, 714, 879, 941, 1137, 1278, 1695
Tru9I	TTAA	8 cut(s)	461, 491, 714, 879, 941, 1137, 1278, 1695
TscAI	CASTG	3 cut(s)	181, 712, 1215
TseFI	GTSAC	3 cut(s)	529, 866, 1549
TseI	GCWGC	3 cut(s)	1523, 1625, 1637
Tsp45I	GTSAC	3 cut(s)	529, 866, 1549
TspDTI	ATGAA	4 cut(s)	345, 754, 1176, 1524
TspRI	CASTG	3 cut(s)	181, 712, 1215
Vha464I	CTTAAG	1 cut(s)	713
VpaK11BI	GGWCC	1 cut(s)	1356
VspI	ATTAAT	1 cut(s)	941
XapI	RAATTY	4 cut(s)	513, 585, 761, 798
XceI	RCATGY	1 cut(s)	645
XmiI	GTMKAC	1 cut(s)	611
XmnI	GAANNNNTTC	2 cut(s)	601, 1125
XspI	CTAG	3 cut(s)	803, 1380, 1424
Zsp2I	ATGCAT	1 cut(s)	838

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.