MD08G1115000.v1.1

Carotenoid 9,10(9',10')-cleavage dioxygenase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0013626
Physical Location & Seq
Forward (+)
10396383 .. 10412824
16442 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1115000.v1.1.491

Sequence Viewer

Length: 1719 bp
ATGGAACAAGTCATTCCCATGCAAATAGATGTCTCCAAAACTTTCAAGAGCACTTCTGACAAAGTGTTGGACTTCTTGGTAGATTCAATGTTCGAATTCGTCGATCAAGAGTATCTTCCATCTCAGAGCAACTTCAGGCCGGTTGATGAGTTGGGAGGAGTAGTTGCTGTCACCAGCAGCATCAGAGGGAAAATCCCAGATGATTTTCCAGAGGGTGTCTACATAAGAAATGGGCCAAACCCTCTATTTGGAGGATTTAAATCGACAAGATCTGTGTTTGGAAGGTCAAGTCACATATGGGTTGAAGGAGAAGGTATGCTACATGCTTTATATTTTAGTAGGGACGGCGACGTCAATGATGGAGGCTGGACTGTCCGGTACAACAACAGACACGTTGAAACGGAAACGTTCAAGCTTGAAAAACAGAGAAACAAGCCGTCTTTTCTTCCCGCCATAGGCGGGGACTCGCCGGCTATTTTGGCCGCTTACCTCCTCAATTTGTTGAGGTTTGGCAAAGTGAACAAGTATTATAGTAACACCAATGTTTTCGAGCATTCGGGGAAGTTCTACTCCGTAGCAGAAAATCACATACCTCAAGAGATTGACATCATTACATTAGAAACTTTAGGCAACTGGGATTTCTCTGGAGCTTGGAACAGACCTTTTACCAGCCACGCTAAGAGAGCTCCAGGCTCAGAGGAGCTGGTCATTCACGGATTTGACGCAGGAAAACCATATATGGAAATTGGAATAATTTCAGCTGATGGAAAGGAGCTAATTCACAAAGTAGATCTCAAACTCAGTAGGAGCAGCTTTTTCCATGAATTAGGGATCACAGAGAGGTATAATGTACTCATGGATTTTCCTCTAACTTTTGACATAAACAGACTCGTTAACGGAGGCCCATTATTAGAGTACAATCATGAAGGATATGCACGAATCGGTGTGATGCCACGCTACGGCGATGCAGATTCAATCTGCTGGTTTAAAGTTGAACCCAATTGCACATTTCACATAATCAATAGTTTTGAAGATGGTGATGAGGTTGTAGTGTGGGGTTGTAAAGCTCTGGACTCAATTGTACCAGGACCTGACATGGCATCAACTCAATTTGGGTGGTTGCCAAGAAAATTTAAGCCTACAAGTCCAAGTAAAGAAAATGATCATGATACTATCTCATCAGTCGACTATGGAAACTTATTTTCTCATGCTTATGAATGGAGATTAAACATGCAAAGTGGGGAAGTCAGTGAGAGATATCTCACTGGAAAAAGTTTTTGTATAGATTTTCCCATGATCAATGGAGCTTTCTCCGGCGTTAAAAATAGATATGGCTACGCCCAAGTGGTTGATTCCGTCGCAAGCTCTGCCTCAGGCATGCTCAAATATAATGGTCTAGCCAAGCTACACTTTGAAGAACCAGCTGATGTTTCTTTGAGAAGTGAAGGCCAACTAGAAGATGCAATAAAGGTGGAAACTCACATGTTTGAGGAGAACAGCTTTTGCACTGGAGTTTCTTTTGTTCCAAAACAAGGGGGTTTTGAAGAAGATGATGGATGGCTTATCACTTTTGTTCACAATGAAGATACCAACATATCTCAAGTTTATGTGATCGAGACAAAGAAGTTTTCCAATGACCCTGTTGCCAAAATTACATTACCCTGTAGAGTTCCATACGGATTTCATGGAGCCTTCATGCCAATCTCATCACCCACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RPE65

Protein Analysis

573

Amino Acids

64.2

Weight (kDa)

5.2

Isoelectric Point (pI)

38.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPE65 PF03055 57 - 567 1.1e-109 Retinal pigment epithelial membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 350
AatII GACGTC 1 cut(s) 354
AccI GTMKAC 2 cut(s) 219, 1185
AciI CCGC 3 cut(s) 450, 459, 483
AclI AACGTT 1 cut(s) 407
AclWI GGATC 1 cut(s) 839
AcoI YGGCCR 1 cut(s) 480
AcsI RAATTY 2 cut(s) 95, 1130
AcuI CTGAAG 1 cut(s) 118
AcyI GRCGYC 1 cut(s) 351
AfaI GTAC 4 cut(s) 380, 852, 917, 1083
AfiI CCNNNNNNNGG 5 cut(s) 248, 455, 459, 959, 1532
AflIII ACRYGT 2 cut(s) 391, 1482
AjnI CCWGG 2 cut(s) 688, 1084
Alw21I GWGCWC 2 cut(s) 53, 688
Alw26I GTCTC 2 cut(s) 37, 1610
AlwI GGATC 1 cut(s) 839
AlwNI CAGNNNCTG 1 cut(s) 1091
AoxI GGCC 5 cut(s) 137, 233, 480, 901, 1447
ApeKI GCWGC 2 cut(s) 177, 810
ApoI RAATTY 2 cut(s) 95, 1130
Asp700I GAANNNNTTC 1 cut(s) 754
AspS9I GGNCC 3 cut(s) 233, 902, 1088
AsuHPI GGTGA 3 cut(s) 163, 1049, 1701
AsuII TTCGAA 1 cut(s) 93
AvaII GGWCC 1 cut(s) 1088
AxyI CCTNAGG 1 cut(s) 1372
BanII GRGCYC 1 cut(s) 688
BarI GAAGNNNNNNTAC 2 cut(s) 303, 335
Bbv12I GWGCWC 2 cut(s) 53, 688
BbvI GCAGC 2 cut(s) 189, 822
BccI CCATC 6 cut(s) 127, 353, 758, 1028, 1547, 1551
BceAI ACGGC 3 cut(s) 361, 421, 976
BciT130I CCWGG 2 cut(s) 690, 1086
BclI TGATCA 2 cut(s) 1162, 1296
BcoDI GTCTC 2 cut(s) 37, 1610
BfaI CTAG 2 cut(s) 1397, 1454
BfmI CTRYAG 1 cut(s) 1663
BglII AGATCT 2 cut(s) 269, 790
BisI GCNGC 3 cut(s) 178, 483, 811
BlsI GCNGC 3 cut(s) 179, 484, 812
Bme1390I CCNGG 2 cut(s) 690, 1086
Bme18I GGWCC 1 cut(s) 1088
BmgT120I GGNCC 3 cut(s) 233, 902, 1088
BmiI GGNNCC 1 cut(s) 1690
BmrFI CCNGG 2 cut(s) 690, 1086
BmrI ACTGGG 1 cut(s) 643
BmsI GCATC 5 cut(s) 189, 939, 955, 1109, 1450
BmuI ACTGGG 1 cut(s) 643
BpmI CTGGAG 3 cut(s) 666, 672, 1530
Bpu14I TTCGAA 1 cut(s) 93
BpuEI CTTGAG 2 cut(s) 579, 1584
BsaBI GATNNNNATC 2 cut(s) 259, 605
BsaHI GRCGYC 1 cut(s) 351
BsaWI WCCGGW 1 cut(s) 375
BsaXI ACNNNNNCTCC 2 cut(s) 147, 177
Bsc4I CCNNNNNNNGG 5 cut(s) 248, 455, 459, 959, 1532
Bse118I RCCGGY 2 cut(s) 139, 469
Bse1I ACTGG 3 cut(s) 638, 1270, 1513
Bse21I CCTNAGG 1 cut(s) 1372
Bse8I GATNNNNATC 2 cut(s) 259, 605
BseBI CCWGG 2 cut(s) 690, 1086
BseGI GGATG 1 cut(s) 1562
BseJI GATNNNNATC 2 cut(s) 259, 605
BseLI CCNNNNNNNGG 5 cut(s) 248, 455, 459, 959, 1532
BseMII CTCAG 4 cut(s) 137, 708, 814, 1386
BseNI ACTGG 3 cut(s) 638, 1270, 1513
BseRI GAGGAG 4 cut(s) 171, 482, 713, 1505
BseXI GCAGC 2 cut(s) 189, 822
BshFI GGCC 5 cut(s) 139, 235, 482, 903, 1449
BsiHKAI GWGCWC 2 cut(s) 53, 688
BsiSI CCGG 4 cut(s) 140, 376, 470, 1314
BslFI GGGAC 2 cut(s) 356, 476
BslI CCNNNNNNNGG 5 cut(s) 248, 455, 459, 959, 1532
BsmAI GTCTC 2 cut(s) 37, 1610
BsmFI GGGAC 2 cut(s) 356, 476
BsmI GAATGC 1 cut(s) 553
BsnI GGCC 5 cut(s) 139, 235, 482, 903, 1449
Bsp119I TTCGAA 1 cut(s) 93
Bsp1286I GDGCHC 2 cut(s) 53, 688
Bsp143I GATC 7 cut(s) 103, 269, 790, 831, 1162, 1296, 1611
BspACI CCGC 3 cut(s) 450, 459, 483
BspANI GGCC 5 cut(s) 139, 235, 482, 903, 1449
BspCNI CTCAG 4 cut(s) 136, 707, 813, 1385
BspHI TCATGA 2 cut(s) 922, 1165
BspLI GGNNCC 1 cut(s) 1690
BspPI GGATC 1 cut(s) 839
BspT104I TTCGAA 1 cut(s) 93
BsrFI RCCGGY 2 cut(s) 139, 469
BsrI ACTGG 3 cut(s) 638, 1270, 1513
BssAI RCCGGY 2 cut(s) 139, 469
BssMI GATC 7 cut(s) 103, 269, 790, 831, 1162, 1296, 1611
BssNI GRCGYC 1 cut(s) 351
Bst2UI CCWGG 2 cut(s) 690, 1086
Bst4CI ACNGT 1 cut(s) 373
BstACI GRCGYC 1 cut(s) 351
BstAPI GCANNNNNTGC 1 cut(s) 1367
BstBI TTCGAA 1 cut(s) 93
BstC8I GCNNGC 3 cut(s) 471, 1363, 1379
BstDEI CTNAG 6 cut(s) 123, 678, 694, 800, 1372, 1716
BstF5I GGATG 1 cut(s) 1562
BstKTI GATC 7 cut(s) 106, 272, 793, 834, 1165, 1299, 1614
BstMAI GTCTC 2 cut(s) 37, 1610
BstMBI GATC 7 cut(s) 103, 269, 790, 831, 1162, 1296, 1611
BstMWI GCNNNNNNNGC 3 cut(s) 479, 683, 1367
BstNI CCWGG 2 cut(s) 690, 1086
BstNSI RCATGY 4 cut(s) 326, 1234, 1381, 1486
BstSCI CCNGG 2 cut(s) 688, 1084
BstSFI CTRYAG 1 cut(s) 1663
BstV1I GCAGC 2 cut(s) 189, 822
BstX2I RGATCY 2 cut(s) 269, 790
BstYI RGATCY 2 cut(s) 269, 790
Bsu36I CCTNAGG 1 cut(s) 1372
BsuRI GGCC 5 cut(s) 139, 235, 482, 903, 1449
BtgZI GCGATG 1 cut(s) 978
BtsCI GGATG 1 cut(s) 1562
BtsIMutI CAGTG 3 cut(s) 1255, 1263, 1506
Cac8I GCNNGC 3 cut(s) 471, 1363, 1379
CaiI CAGNNNCTG 1 cut(s) 1091
CciI TCATGA 2 cut(s) 922, 1165
Cfr10I RCCGGY 2 cut(s) 139, 469
Cfr13I GGNCC 3 cut(s) 233, 902, 1088
CseI GACGC 1 cut(s) 731
Csp6I GTAC 4 cut(s) 379, 851, 916, 1082
CspCI CAANNNNNGTGG 4 cut(s) 1097, 1132, 1452, 1487
CviQI GTAC 4 cut(s) 379, 851, 916, 1082
DdeI CTNAG 6 cut(s) 123, 678, 694, 800, 1372, 1716
DpnI GATC 7 cut(s) 105, 271, 792, 833, 1164, 1298, 1613
DpnII GATC 7 cut(s) 103, 269, 790, 831, 1162, 1296, 1611
DraI TTTAAA 2 cut(s) 259, 988
DrdI GACNNNNNNGTC 1 cut(s) 350
DseDI GACNNNNNNGTC 1 cut(s) 350
EaeI YGGCCR 1 cut(s) 480
Ecl136II GAGCTC 1 cut(s) 686
Eco24I GRGCYC 1 cut(s) 688
Eco32I GATATC 1 cut(s) 1259
Eco47I GGWCC 1 cut(s) 1088
Eco53kI GAGCTC 1 cut(s) 686
Eco57I CTGAAG 1 cut(s) 118
Eco81I CCTNAGG 1 cut(s) 1372
EcoICRI GAGCTC 1 cut(s) 686
EcoO109I RGGNCCY 1 cut(s) 1088
EcoRI GAATTC 1 cut(s) 95
EcoRII CCWGG 2 cut(s) 688, 1084
EcoRV GATATC 1 cut(s) 1259
EcoT38I GRGCYC 1 cut(s) 688
FalI AAGNNNNNCTT 4 cut(s) 99, 131, 1394, 1426
FaqI GGGAC 2 cut(s) 356, 476
FauI CCCGC 2 cut(s) 452, 457
FauNDI CATATG 1 cut(s) 296
FbaI TGATCA 2 cut(s) 1162, 1296
FblI GTMKAC 2 cut(s) 219, 1185
Fnu4HI GCNGC 3 cut(s) 178, 483, 811
FokI GGATG 1 cut(s) 1569
FriOI GRGCYC 1 cut(s) 688
Fsp4HI GCNGC 3 cut(s) 178, 483, 811
FspBI CTAG 2 cut(s) 1397, 1454
GluI GCNGC 3 cut(s) 178, 483, 811
GsuI CTGGAG 3 cut(s) 666, 672, 1530
HaeIII GGCC 5 cut(s) 139, 235, 482, 903, 1449
HapII CCGG 4 cut(s) 140, 376, 470, 1314
HgaI GACGC 1 cut(s) 731
Hin1I GRCGYC 1 cut(s) 351
HincII GTYRAC 2 cut(s) 895, 1186
HindII GTYRAC 2 cut(s) 895, 1186
HindIII AAGCTT 1 cut(s) 413
HinfI GANTC 7 cut(s) 83, 464, 888, 939, 971, 1073, 1352
HpaI GTTAAC 1 cut(s) 895
HpaII CCGG 4 cut(s) 140, 376, 470, 1314
HphI GGTGA 3 cut(s) 163, 1049, 1701
Hpy166II GTNNAC 5 cut(s) 220, 520, 895, 1186, 1576
Hpy188I TCNGA 4 cut(s) 58, 126, 185, 697
Hpy188III TCNNGA 9 cut(s) 46, 107, 209, 596, 645, 923, 1070, 1166, 1615
Hpy8I GTNNAC 5 cut(s) 220, 520, 895, 1186, 1576
Hpy99I CGWCG 3 cut(s) 104, 353, 1361
HpyAV CCTTC 6 cut(s) 276, 299, 305, 920, 1439, 1702
HpyCH4III ACNGT 1 cut(s) 373
HpyCH4IV ACGT 3 cut(s) 351, 393, 407
HpyCH4V TGCA 7 cut(s) 22, 935, 968, 1005, 1234, 1463, 1506
HpyF10VI GCNNNNNNNGC 3 cut(s) 479, 683, 1367
HpyF3I CTNAG 6 cut(s) 123, 678, 694, 800, 1372, 1716
HpySE526I ACGT 3 cut(s) 351, 393, 407
Hsp92I GRCGYC 1 cut(s) 351
KroI GCCGGC 1 cut(s) 469
KroNI GCCGGC 1 cut(s) 471
Ksp22I TGATCA 2 cut(s) 1162, 1296
KspAI GTTAAC 1 cut(s) 895
Kzo9I GATC 7 cut(s) 103, 269, 790, 831, 1162, 1296, 1611
LmnI GCTCC 7 cut(s) 647, 691, 700, 772, 807, 1304, 1688
Lsp1109I GCAGC 2 cut(s) 189, 822
LweI GCATC 5 cut(s) 189, 939, 955, 1109, 1450
MaeI CTAG 2 cut(s) 1397, 1454
MaeII ACGT 3 cut(s) 351, 393, 407
MaeIII GTNAC 3 cut(s) 169, 290, 533
MalI GATC 7 cut(s) 105, 271, 792, 833, 1164, 1298, 1613
MboI GATC 7 cut(s) 103, 269, 790, 831, 1162, 1296, 1611
MboII GAAGA 8 cut(s) 107, 437, 1043, 1427, 1469, 1556, 1559, 1595
MfeI CAATTG 2 cut(s) 1000, 1077
MflI RGATCY 2 cut(s) 269, 790
MhlI GDGCHC 2 cut(s) 53, 688
MlyI GAGTC 3 cut(s) 458, 882, 1067
MmeI TCCRAC 1 cut(s) 48
MroNI GCCGGC 1 cut(s) 469
MroXI GAANNNNTTC 1 cut(s) 754
MseI TTAA 6 cut(s) 258, 894, 987, 1134, 1226, 1320
MslI CAYNNNNRTG 2 cut(s) 17, 1212
MspA1I CMGCKG 2 cut(s) 761, 1424
MspI CCGG 4 cut(s) 140, 376, 470, 1314
MspR9I CCNGG 2 cut(s) 690, 1086
MunI CAATTG 2 cut(s) 1000, 1077
Mva1269I GAATGC 1 cut(s) 553
MvaI CCWGG 2 cut(s) 690, 1086
MwoI GCNNNNNNNGC 3 cut(s) 479, 683, 1367
NaeI GCCGGC 1 cut(s) 471
NdeI CATATG 1 cut(s) 296
NdeII GATC 7 cut(s) 103, 269, 790, 831, 1162, 1296, 1611
NgoMIV GCCGGC 1 cut(s) 469
NlaIV GGNNCC 1 cut(s) 1690
NmuCI GTSAC 2 cut(s) 169, 290
NspI RCATGY 4 cut(s) 326, 1234, 1381, 1486
NspV TTCGAA 1 cut(s) 93
PaeI GCATGC 1 cut(s) 1381
PagI TCATGA 2 cut(s) 922, 1165
PciI ACATGT 1 cut(s) 1482
PcsI WCGNNNNNNNCGW 1 cut(s) 99
PctI GAATGC 1 cut(s) 553
PdiI GCCGGC 1 cut(s) 471
PdmI GAANNNNTTC 1 cut(s) 754
PfeI GAWTC 4 cut(s) 83, 939, 971, 1352
PkrI GCNGC 3 cut(s) 179, 484, 812
PleI GAGTC 3 cut(s) 458, 882, 1067
PpsI GAGTC 3 cut(s) 458, 882, 1067
PpuMI RGGWCCY 1 cut(s) 1088
PscI ACATGT 1 cut(s) 1482
Psp124BI GAGCTC 1 cut(s) 688
Psp1406I AACGTT 1 cut(s) 407
Psp5II RGGWCCY 1 cut(s) 1088
Psp6I CCWGG 2 cut(s) 688, 1084
PspGI CCWGG 2 cut(s) 688, 1084
PspN4I GGNNCC 1 cut(s) 1690
PspPI GGNCC 3 cut(s) 233, 902, 1088
PspPPI RGGWCCY 1 cut(s) 1088
PstNI CAGNNNCTG 1 cut(s) 1091
PsuI RGATCY 2 cut(s) 269, 790
PvuII CAGCTG 2 cut(s) 761, 1424
RsaI GTAC 4 cut(s) 380, 852, 917, 1083
RsaNI GTAC 4 cut(s) 379, 851, 916, 1082
RseI CAYNNNNRTG 2 cut(s) 17, 1212
SacI GAGCTC 1 cut(s) 688
SalI GTCGAC 1 cut(s) 1184
SaqAI TTAA 6 cut(s) 258, 894, 987, 1134, 1226, 1320
SatI GCNGC 3 cut(s) 178, 483, 811
Sau3AI GATC 7 cut(s) 103, 269, 790, 831, 1162, 1296, 1611
Sau96I GGNCC 3 cut(s) 233, 902, 1088
SchI GAGTC 3 cut(s) 458, 882, 1067
ScrFI CCNGG 2 cut(s) 690, 1086
SduI GDGCHC 2 cut(s) 53, 688
SfaNI GCATC 5 cut(s) 189, 939, 955, 1109, 1450
SfcI CTRYAG 1 cut(s) 1663
SfuI TTCGAA 1 cut(s) 93
SinI GGWCC 1 cut(s) 1088
SmiI ATTTAAAT 1 cut(s) 259
SmiMI CAYNNNNRTG 2 cut(s) 17, 1212
SmlI CTYRAG 2 cut(s) 594, 1599
SmoI CTYRAG 2 cut(s) 594, 1599
SphI GCATGC 1 cut(s) 1381
SsiI CCGC 3 cut(s) 450, 459, 483
SspMI CTAG 2 cut(s) 1397, 1454
SstI GAGCTC 1 cut(s) 688
StyD4I CCNGG 2 cut(s) 688, 1084
SwaI ATTTAAAT 1 cut(s) 259
TaaI ACNGT 1 cut(s) 373
TaiI ACGT 3 cut(s) 354, 396, 410
TaqI TCGA 6 cut(s) 93, 102, 263, 549, 1185, 1614
TatI WGTACW 2 cut(s) 850, 915
TauI GCSGC 1 cut(s) 485
TfiI GAWTC 4 cut(s) 83, 939, 971, 1352
Tru1I TTAA 6 cut(s) 258, 894, 987, 1134, 1226, 1320
Tru9I TTAA 6 cut(s) 258, 894, 987, 1134, 1226, 1320
TscAI CASTG 3 cut(s) 1255, 1270, 1513
TseFI GTSAC 2 cut(s) 169, 290
TseI GCWGC 2 cut(s) 177, 810
Tsp45I GTSAC 2 cut(s) 169, 290
TspDTI ATGAA 6 cut(s) 837, 939, 1230, 1596, 1673, 1684
TspGWI ACGGA 6 cut(s) 416, 562, 729, 912, 1345, 1692
TspRI CASTG 3 cut(s) 1255, 1270, 1513
VpaK11BI GGWCC 1 cut(s) 1088
XapI RAATTY 2 cut(s) 95, 1130
XceI RCATGY 4 cut(s) 326, 1234, 1381, 1486
XmiI GTMKAC 2 cut(s) 219, 1185
XmnI GAANNNNTTC 1 cut(s) 754
XspI CTAG 2 cut(s) 1397, 1454
ZraI GACGTC 1 cut(s) 352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.