RchiOBHm_Chr6g0306131

Carotenoid 9,10(9',10')-cleavage dioxygenase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0013626
Physical Location & Seq
Reverse (-)
64887394 .. 64891334
3941 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27515

Sequence Viewer

Length: 1692 bp
ATGCAACAAGTAATTCCCATGCATATAGATGTCTCGAAAATCTTCAAGAACGCTTCTGCAAAAATGTTGGACGCTTTTGTAGATTCGGTATTTGAATTCATTGATCAACCATTGCTTCCATCTCAGAACTTCGCCCCAGTTGGTGAATTGGAAGGAGAAGGAGTTGTAATCACGAGCATCATCGGAAGAATTCCAGATAACTTTCCGGAGGGAGTCTACGTAAGAAATGGACCAAACCCTTTATTTGGAGGATTGAAATCGACGAAATCAGTGTTTGGAAGGTCAAGTCATATATGGATAGAAGGAGAAGGCATGGTTCATGGTCTGTATTTTACTAAGAACGACGTCGTCAGCAGTGATGGTAGCAGATGGACGGTCCGGTACAACAACAGACATGTCGAAACCGAAACTTACAAGCTTGAAAAACAAAGAAACAAGCCTTCTTTTCTCCCTGCCATTGGTGGCGACTCGCCGGCTATTTTGTCTGCTTATCTCCTCAATTTGTTGCGGTTTGGCAAAGTAAACAAGTACTTAAGCAACACAAATGTGTTTGAGCATTCGGGGAGGTTTTACTCAGTTGCAGAAAATCACATACCACAAGAGATTGATATCCTTACGCTGGAAACTTTAGGAAATTGGGATGTCTCTGGAGCTTGGAACAGACCTTTTATTAGCCACCCGAAGGTACGCACAGGAGAGTTGGTCATAATGGGCGTGGACGCAGTTAAACCGTTTTGGGAAGTAGGGTCACTTTATTTAATAGCTGATGGCAAAGAATTAATCCACAAGGTGGATCTCAAACTCAATAGGTGCAGCATTTGTCATGAAATAGGGGTTACGCAGAGGTACAATGTGATCATGGATATTGCACTAACTATAGACATAAAGAGACTCGTAGGTGGAGGCCCATTAGTAAAGTATAATGAAGAAGAATATGCAAGAATTTGTGTAATGCCTCGCTACGGTGACGCAAGTTCAATCCGGTGGTTTAAAGTTGAACCCAATTGCACATTTCACATCATCAATAGTTTTGAAGCTGGTGATGAGGTTGTAGTGTGGGGTTGTAAAGCTCTTGACTCAGTTATAGGTCCAAGTAATGAAAGCAGTACTGATAATAGCACCTCAACGGCAGAGGATGCTAGTAAATTATACTCCCATGCTTATGAATGGAGATTAAACATGGAGACTGGAGAAGTCAGGGAGAGATATCTCACTGGCAAAAAGTTTTCCATGGATTTTCCTACGATCAATGGAAACTTTTGCGGCCTTAAAAACAGATTTGGGTATGCCCAAGTCGTCGATTCAGTTGCGAGCTCTACCTCAGGGTTGCTTAAGTATGGTGGTATAGCTAAGCTACATTTTGAAGAGCCAGCTGAGAGAGAAGGCGAATTAGAAAATGAGGAAATAAAGGTAGAAAGACATATGTTTGAGGAGAACACCTTTTGTACCGGAGCTGCTTTTGTCCCTAAACAAAGTGATAGTAGCAGGACTGGTAGTATTATTCTTGAAGAAGTAGAAGAAGATGATGGATGGGTTATTACTTTTGTTCACAATGAAGATACCAAAGTATCACAAGTTTATGTTATTGATACCAAGGAGTTTTGCAGTGAGCCTGTAGCCAAAATTACATTGCCCTGCAGAGTTCCATATGGATTTCATGGAGCTTTCATGCCAACCTCATCGAACACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RPE65

Protein Analysis

563

Amino Acids

63.05

Weight (kDa)

5.34

Isoelectric Point (pI)

38.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPE65 PF03055 50 - 558 2e-106 Retinal pigment epithelial membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 348
AccB7I CCANNNNNTGG 1 cut(s) 790
AccI GTMKAC 1 cut(s) 216
AccIII TCCGGA 1 cut(s) 205
AciI CCGC 2 cut(s) 508, 1263
AclWI GGATC 1 cut(s) 801
AcsI RAATTY 3 cut(s) 95, 189, 942
AcyI GRCGYC 1 cut(s) 345
AdeI CACNNNGTG 1 cut(s) 790
AfaI GTAC 6 cut(s) 383, 530, 687, 848, 1108, 1447
AfiI CCNNNNNNNGG 6 cut(s) 245, 458, 619, 682, 790, 962
AflII CTTAAG 2 cut(s) 532, 1331
AflIII ACRYGT 1 cut(s) 394
AgsI TTSAA 9 cut(s) 46, 95, 256, 422, 978, 998, 1034, 1364, 1508
AleI CACNNNNGTG 1 cut(s) 545
Alw21I GWGCWC 1 cut(s) 1316
Alw26I GTCTC 4 cut(s) 37, 649, 883, 1178
AlwI GGATC 1 cut(s) 801
Aor13HI TCCGGA 1 cut(s) 205
AoxI GGCC 2 cut(s) 904, 1264
ApeKI GCWGC 2 cut(s) 813, 1454
ApoI RAATTY 3 cut(s) 95, 189, 942
AseI ATTAAT 1 cut(s) 779
Asp700I GAANNNNTTC 1 cut(s) 41
AspS9I GGNCC 4 cut(s) 230, 376, 905, 1088
AsuHPI GGTGA 3 cut(s) 155, 977, 1052
AvaII GGWCC 3 cut(s) 230, 376, 1088
AxyI CCTNAGG 1 cut(s) 1321
BaeI ACNNNNGTAYC 4 cut(s) 1551, 1551, 1584, 1584
BanII GRGCYC 1 cut(s) 1316
BauI CACGAG 1 cut(s) 172
Bbv12I GWGCWC 1 cut(s) 1316
BbvI GCAGC 2 cut(s) 825, 1441
BccI CCATC 6 cut(s) 127, 353, 363, 761, 1520, 1524
BceAI ACGGC 1 cut(s) 1143
BcgI CGANNNNNNTGC 2 cut(s) 1289, 1323
BclI TGATCA 2 cut(s) 103, 855
BcoDI GTCTC 4 cut(s) 37, 649, 883, 1178
BfaI CTAG 1 cut(s) 1140
BfmI CTRYAG 3 cut(s) 876, 1614, 1636
BfrI CTTAAG 2 cut(s) 532, 1331
BisI GCNGC 3 cut(s) 814, 1264, 1455
BlpI GCTNAGC 1 cut(s) 1350
BlsI GCNGC 3 cut(s) 815, 1265, 1456
BmcAI AGTACT 2 cut(s) 530, 1108
Bme18I GGWCC 3 cut(s) 230, 376, 1088
BmgT120I GGNCC 4 cut(s) 230, 376, 905, 1088
BmrI ACTGGG 1 cut(s) 131
BmsI GCATC 2 cut(s) 186, 1126
BmuI ACTGGG 1 cut(s) 131
BpmI CTGGAG 2 cut(s) 669, 1209
Bpu1102I GCTNAGC 1 cut(s) 1350
BsaAI YACGTR 1 cut(s) 220
BsaBI GATNNNNATC 2 cut(s) 256, 608
BsaHI GRCGYC 1 cut(s) 345
BsaJI CCNNGG 2 cut(s) 1230, 1593
BsaWI WCCGGW 4 cut(s) 205, 378, 981, 1448
BsaXI ACNNNNNCTCC 6 cut(s) 147, 177, 204, 234, 687, 717
Bsc4I CCNNNNNNNGG 6 cut(s) 245, 458, 619, 682, 790, 962
Bse118I RCCGGY 1 cut(s) 472
Bse1I ACTGG 4 cut(s) 137, 1192, 1219, 1495
Bse21I CCTNAGG 1 cut(s) 1321
Bse3DI GCAATG 2 cut(s) 110, 1628
Bse8I GATNNNNATC 2 cut(s) 256, 608
BseAI TCCGGA 1 cut(s) 205
BseDI CCNNGG 2 cut(s) 1230, 1593
BseGI GGATG 3 cut(s) 646, 1141, 1535
BseJI GATNNNNATC 2 cut(s) 256, 608
BseLI CCNNNNNNNGG 6 cut(s) 245, 458, 619, 682, 790, 962
BseMI GCAATG 2 cut(s) 110, 1628
BseMII CTCAG 5 cut(s) 137, 588, 1092, 1335, 1365
BseNI ACTGG 4 cut(s) 137, 1192, 1219, 1495
BseRI GAGGAG 2 cut(s) 485, 1445
BseXI GCAGC 2 cut(s) 825, 1441
BsgI GTGCAG 1 cut(s) 832
BshFI GGCC 2 cut(s) 906, 1266
BsiHKAI GWGCWC 1 cut(s) 1316
BsiSI CCGG 5 cut(s) 206, 379, 473, 982, 1449
BslFI GGGAC 1 cut(s) 1448
BslI CCNNNNNNNGG 6 cut(s) 245, 458, 619, 682, 790, 962
BsmAI GTCTC 4 cut(s) 37, 649, 883, 1178
BsmFI GGGAC 1 cut(s) 1448
BsmI GAATGC 1 cut(s) 556
BsnI GGCC 2 cut(s) 906, 1266
Bsp1286I GDGCHC 1 cut(s) 1316
Bsp13I TCCGGA 1 cut(s) 205
Bsp143I GATC 4 cut(s) 103, 793, 855, 1245
Bsp1720I GCTNAGC 1 cut(s) 1350
Bsp19I CCATGG 1 cut(s) 1230
BspACI CCGC 2 cut(s) 508, 1263
BspANI GGCC 2 cut(s) 906, 1266
BspCNI CTCAG 5 cut(s) 136, 587, 1091, 1334, 1366
BspEI TCCGGA 1 cut(s) 205
BspHI TCATGA 1 cut(s) 823
BspMAI CTGCAG 1 cut(s) 1640
BspPI GGATC 1 cut(s) 801
BspQI GCTCTTC 1 cut(s) 1359
BspTI CTTAAG 2 cut(s) 532, 1331
BsrDI GCAATG 2 cut(s) 110, 1628
BsrFI RCCGGY 1 cut(s) 472
BsrI ACTGG 4 cut(s) 137, 1192, 1219, 1495
BssAI RCCGGY 1 cut(s) 472
BssECI CCNNGG 2 cut(s) 1230, 1593
BssMI GATC 4 cut(s) 103, 793, 855, 1245
BssNI GRCGYC 1 cut(s) 345
BssSI CACGAG 1 cut(s) 172
BssT1I CCWWGG 2 cut(s) 1230, 1593
Bst2BI CACGAG 1 cut(s) 172
Bst4CI ACNGT 3 cut(s) 376, 732, 965
Bst6I CTCTTC 1 cut(s) 1359
BstACI GRCGYC 1 cut(s) 345
BstAFI CTTAAG 2 cut(s) 532, 1331
BstAPI GCANNNNNTGC 1 cut(s) 1136
BstBAI YACGTR 1 cut(s) 220
BstC8I GCNNGC 3 cut(s) 474, 1312, 1371
BstDEI CTNAG 7 cut(s) 123, 336, 574, 1078, 1321, 1350, 1374
BstDSI CCRYGG 1 cut(s) 1230
BstF5I GGATG 3 cut(s) 646, 1141, 1535
BstKTI GATC 4 cut(s) 106, 796, 858, 1248
BstMAI GTCTC 4 cut(s) 37, 649, 883, 1178
BstMBI GATC 4 cut(s) 103, 793, 855, 1245
BstMWI GCNNNNNNNGC 1 cut(s) 1136
BstNSI RCATGY 1 cut(s) 398
BstSFI CTRYAG 3 cut(s) 876, 1614, 1636
BstSNI TACGTA 1 cut(s) 220
BstV1I GCAGC 2 cut(s) 825, 1441
BstX2I RGATCY 1 cut(s) 793
BstYI RGATCY 1 cut(s) 793
Bsu36I CCTNAGG 1 cut(s) 1321
BsuRI GGCC 2 cut(s) 906, 1266
BtgI CCRYGG 1 cut(s) 1230
BtsCI GGATG 3 cut(s) 646, 1141, 1535
BtsI GCAGTG 2 cut(s) 361, 1612
BtsIMutI CAGTG 4 cut(s) 276, 361, 1212, 1612
Cac8I GCNNGC 3 cut(s) 474, 1312, 1371
CciI TCATGA 1 cut(s) 823
Cfr10I RCCGGY 1 cut(s) 472
Cfr13I GGNCC 4 cut(s) 230, 376, 905, 1088
CpoI CGGWCCG 1 cut(s) 376
CseI GACGC 3 cut(s) 80, 728, 977
Csp6I GTAC 6 cut(s) 382, 529, 686, 847, 1107, 1446
CspI CGGWCCG 1 cut(s) 376
CviQI GTAC 6 cut(s) 382, 529, 686, 847, 1107, 1446
DdeI CTNAG 7 cut(s) 123, 336, 574, 1078, 1321, 1350, 1374
DpnI GATC 4 cut(s) 105, 795, 857, 1247
DpnII GATC 4 cut(s) 103, 793, 855, 1245
DraI TTTAAA 1 cut(s) 991
DraIII CACNNNGTG 1 cut(s) 790
Eam1104I CTCTTC 1 cut(s) 1359
EarI CTCTTC 1 cut(s) 1359
Ecl136II GAGCTC 1 cut(s) 1314
Eco105I TACGTA 1 cut(s) 220
Eco130I CCWWGG 2 cut(s) 1230, 1593
Eco24I GRGCYC 1 cut(s) 1316
Eco32I GATATC 2 cut(s) 610, 1208
Eco47I GGWCC 3 cut(s) 230, 376, 1088
Eco53kI GAGCTC 1 cut(s) 1314
Eco81I CCTNAGG 1 cut(s) 1321
EcoICRI GAGCTC 1 cut(s) 1314
EcoRI GAATTC 2 cut(s) 95, 189
EcoRV GATATC 2 cut(s) 610, 1208
EcoT14I CCWWGG 2 cut(s) 1230, 1593
EcoT22I ATGCAT 1 cut(s) 24
EcoT38I GRGCYC 1 cut(s) 1316
ErhI CCWWGG 2 cut(s) 1230, 1593
FaqI GGGAC 1 cut(s) 1448
FauNDI CATATG 2 cut(s) 1422, 1648
FbaI TGATCA 2 cut(s) 103, 855
FblI GTMKAC 1 cut(s) 216
Fnu4HI GCNGC 3 cut(s) 814, 1264, 1455
FokI GGATG 3 cut(s) 653, 1148, 1542
FriOI GRGCYC 1 cut(s) 1316
Fsp4HI GCNGC 3 cut(s) 814, 1264, 1455
FspBI CTAG 1 cut(s) 1140
GluI GCNGC 3 cut(s) 814, 1264, 1455
GsuI CTGGAG 2 cut(s) 669, 1209
HaeIII GGCC 2 cut(s) 906, 1266
HapII CCGG 5 cut(s) 206, 379, 473, 982, 1449
HgaI GACGC 3 cut(s) 80, 728, 977
Hin1I GRCGYC 1 cut(s) 345
HindIII AAGCTT 1 cut(s) 416
HinfI GANTC 6 cut(s) 83, 213, 467, 891, 1076, 1301
HpaII CCGG 5 cut(s) 206, 379, 473, 982, 1449
HphI GGTGA 3 cut(s) 155, 977, 1052
Hpy166II GTNNAC 4 cut(s) 217, 523, 718, 1549
Hpy188I TCNGA 2 cut(s) 126, 185
Hpy188III TCNNGA 9 cut(s) 34, 46, 172, 194, 206, 648, 824, 1073, 1505
Hpy8I GTNNAC 4 cut(s) 217, 523, 718, 1549
Hpy99I CGWCG 4 cut(s) 265, 347, 350, 1301
HpyAV CCTTC 8 cut(s) 146, 152, 273, 296, 302, 450, 676, 1376
HpyCH4III ACNGT 3 cut(s) 376, 732, 965
HpyCH4IV ACGT 2 cut(s) 219, 345
HpyF10VI GCNNNNNNNGC 1 cut(s) 1136
HpyF3I CTNAG 7 cut(s) 123, 336, 574, 1078, 1321, 1350, 1374
HpySE526I ACGT 2 cut(s) 219, 345
Hsp92I GRCGYC 1 cut(s) 345
Kpn2I TCCGGA 1 cut(s) 205
KroI GCCGGC 1 cut(s) 472
KroNI GCCGGC 1 cut(s) 474
Ksp22I TGATCA 2 cut(s) 103, 855
Kzo9I GATC 4 cut(s) 103, 793, 855, 1245
LguI GCTCTTC 1 cut(s) 1359
LmnI GCTCC 3 cut(s) 650, 1451, 1661
Lsp1109I GCAGC 2 cut(s) 825, 1441
LweI GCATC 2 cut(s) 186, 1126
MaeI CTAG 1 cut(s) 1140
MaeII ACGT 2 cut(s) 219, 345
MaeIII GTNAC 3 cut(s) 747, 835, 965
MalI GATC 4 cut(s) 105, 795, 857, 1247
MboI GATC 4 cut(s) 103, 793, 855, 1245
MboII GAAGA 9 cut(s) 34, 198, 938, 941, 1376, 1520, 1529, 1532, 1568
MfeI CAATTG 1 cut(s) 1003
MflI RGATCY 1 cut(s) 793
MhlI GDGCHC 1 cut(s) 1316
MlyI GAGTC 4 cut(s) 222, 461, 885, 1070
MmeI TCCRAC 1 cut(s) 48
Mph1103I ATGCAT 1 cut(s) 24
MroI TCCGGA 1 cut(s) 205
MroNI GCCGGC 1 cut(s) 472
MroXI GAANNNNTTC 1 cut(s) 41
MseI TTAA 8 cut(s) 533, 726, 758, 779, 990, 1175, 1269, 1332
MslI CAYNNNNRTG 3 cut(s) 27, 545, 1161
MspA1I CMGCKG 1 cut(s) 1373
MspCI CTTAAG 2 cut(s) 532, 1331
MspI CCGG 5 cut(s) 206, 379, 473, 982, 1449
MunI CAATTG 1 cut(s) 1003
Mva1269I GAATGC 1 cut(s) 556
MwoI GCNNNNNNNGC 1 cut(s) 1136
NaeI GCCGGC 1 cut(s) 474
NcoI CCATGG 1 cut(s) 1230
NdeI CATATG 2 cut(s) 1422, 1648
NdeII GATC 4 cut(s) 103, 793, 855, 1245
NgoMIV GCCGGC 1 cut(s) 472
NmuCI GTSAC 2 cut(s) 747, 965
NsiI ATGCAT 1 cut(s) 24
NspI RCATGY 1 cut(s) 398
OliI CACNNNNGTG 1 cut(s) 545
PagI TCATGA 1 cut(s) 823
PciI ACATGT 1 cut(s) 394
PciSI GCTCTTC 1 cut(s) 1359
PctI GAATGC 1 cut(s) 556
PdiI GCCGGC 1 cut(s) 474
PdmI GAANNNNTTC 1 cut(s) 41
PfeI GAWTC 2 cut(s) 83, 1301
PflFI GACNNNGTC 1 cut(s) 347
PflMI CCANNNNNTGG 1 cut(s) 790
PkrI GCNGC 3 cut(s) 815, 1265, 1456
PleI GAGTC 4 cut(s) 221, 461, 885, 1070
PpsI GAGTC 4 cut(s) 221, 461, 885, 1070
Ppu21I YACGTR 1 cut(s) 220
PscI ACATGT 1 cut(s) 394
PshBI ATTAAT 1 cut(s) 779
Psp124BI GAGCTC 1 cut(s) 1316
PspPI GGNCC 4 cut(s) 230, 376, 905, 1088
PstI CTGCAG 1 cut(s) 1640
PsuI RGATCY 1 cut(s) 793
PsyI GACNNNGTC 1 cut(s) 347
PvuII CAGCTG 1 cut(s) 1373
RsaI GTAC 6 cut(s) 383, 530, 687, 848, 1108, 1447
RsaNI GTAC 6 cut(s) 382, 529, 686, 847, 1107, 1446
RseI CAYNNNNRTG 3 cut(s) 27, 545, 1161
Rsr2I CGGWCCG 1 cut(s) 376
RsrII CGGWCCG 1 cut(s) 376
SacI GAGCTC 1 cut(s) 1316
SapI GCTCTTC 1 cut(s) 1359
SaqAI TTAA 8 cut(s) 533, 726, 758, 779, 990, 1175, 1269, 1332
SatI GCNGC 3 cut(s) 814, 1264, 1455
Sau3AI GATC 4 cut(s) 103, 793, 855, 1245
Sau96I GGNCC 4 cut(s) 230, 376, 905, 1088
ScaI AGTACT 2 cut(s) 530, 1108
SchI GAGTC 4 cut(s) 222, 461, 885, 1070
SduI GDGCHC 1 cut(s) 1316
SfaNI GCATC 2 cut(s) 186, 1126
SfcI CTRYAG 3 cut(s) 876, 1614, 1636
SinI GGWCC 3 cut(s) 230, 376, 1088
SmiMI CAYNNNNRTG 3 cut(s) 27, 545, 1161
SmlI CTYRAG 2 cut(s) 532, 1331
SmoI CTYRAG 2 cut(s) 532, 1331
SnaBI TACGTA 1 cut(s) 220
SsiI CCGC 2 cut(s) 508, 1263
SspMI CTAG 1 cut(s) 1140
SstI GAGCTC 1 cut(s) 1316
StyI CCWWGG 2 cut(s) 1230, 1593
TaaI ACNGT 3 cut(s) 376, 732, 965
TaiI ACGT 2 cut(s) 222, 348
TaqI TCGA 5 cut(s) 35, 260, 399, 1299, 1682
TatI WGTACW 2 cut(s) 528, 1106
TauI GCSGC 1 cut(s) 1266
TfiI GAWTC 2 cut(s) 83, 1301
Tru1I TTAA 8 cut(s) 533, 726, 758, 779, 990, 1175, 1269, 1332
Tru9I TTAA 8 cut(s) 533, 726, 758, 779, 990, 1175, 1269, 1332
TscAI CASTG 4 cut(s) 276, 361, 1219, 1612
TseFI GTSAC 2 cut(s) 747, 965
TseI GCWGC 2 cut(s) 813, 1454
Tsp45I GTSAC 2 cut(s) 747, 965
TspDTI ATGAA 9 cut(s) 88, 308, 840, 939, 1113, 1179, 1569, 1646, 1657
TspRI CASTG 4 cut(s) 276, 361, 1219, 1612
Tth111I GACNNNGTC 1 cut(s) 347
Van91I CCANNNNNTGG 1 cut(s) 790
Vha464I CTTAAG 2 cut(s) 532, 1331
VpaK11BI GGWCC 3 cut(s) 230, 376, 1088
VspI ATTAAT 1 cut(s) 779
XapI RAATTY 3 cut(s) 95, 189, 942
XceI RCATGY 1 cut(s) 398
XmiI GTMKAC 1 cut(s) 216
XmnI GAANNNNTTC 1 cut(s) 41
XspI CTAG 1 cut(s) 1140
ZraI GACGTC 1 cut(s) 346
ZrmI AGTACT 2 cut(s) 530, 1108
Zsp2I ATGCAT 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.