MD08G1115100.v1.1

Carotenoid 9,10(9',10')-cleavage dioxygenase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0013626
Physical Location & Seq
Forward (+)
10425960 .. 10430006
4047 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1115100.v1.1.491

Sequence Viewer

Length: 1320 bp
ATGGCGTTATATTTTTTCAATTTACATGGATCGGGCCTAATTGGGCCAAACCCTCTATTTGGAGGATTGAAATCGACAAGATCTGTGTTTGGAAGGTCAAGTCACGTATGGGTTGAAGGAGAAGGCATGCTACATGCGTTATATTTTAGTAGGGACGGCGACGTTAGTGATGGAGGCTGGACTGTCCGGTACAACAACAGACACGTTGAAACCGACACGTTCAAGCTCGAAAAACAGCGAAACAAGCCGTCTTTTCTCCCCACCTTTAGCGGCGACTCGCGGGCTATTTTGTCCGCTTTCCTCCTCAATTTGTTGAGGTTTGGTAAAGCGAACAAGTATTTGAGCAACACCAATGTTTTCGAGCATTCGGGGAAGTTCTACTCCATAACAGAAGATCACATACCTCAAGAGATTGACATCATCACATTAGAAACTTTGGACAATTGGGATGTCTCTGGAGCTTGGAACAGACCTTTTACCACCCACCCTAAGGTTTTGATATGCAGATTATTAGAGTACAATCATGAAGGATGTGCACGAATCGGTGTGATGCCACACTACGGCGATGCAGATTCAATCCGCTGGTTTAAAGTTGAACCCAGTTGCACATCTCACATCATCAATAGTTTTGAAGATGGTGAAGAGGTTGTAGTGTGGGGTTGTAAAGCTCTGGACTCAGTTATACCAGGACCGGACAAGGCATTGAATCAATTTGGGTGGCCACCAAGAAAATTTAAGCCTACAATTCCAAGTAAAGAAAATGATCATGATAATATCTCACCAATCGACTATGAAAACTTATTTTCTCATGCTTATGAATGGAGATTAAACATGCAAAGTGGGGAAGTCAGTGAAAGATATCTCACTGGAAAAAGGTTTTGCATGGATTTTCCCATGATCAACGGAGCTTTTTCCGGTATCAGAAATAGATATGGCTACGCCCAAGTGGTTGATTCTGCCGCAAGCTCTGCCTCAGGCGTGCTCAAATATAATGGTCTAGCCAAGCTACACTTTGAAGAACCAGCTGATGTTTCTTTGACAAGTGAAGGCCAATTAGAAGAGGCAATAAAGGTGGAAACTCACATGTTTGAGGAGAATAGCTTTTGCACTGGAGCTTCTTTTGTCCCAAAACAAAGGGGTCTTGAAGAAGATGATGGATGGGTTATCACTTTTGTTCACAATGAAGATACCAACATATCTCAAGCTTATGTGATCGATACAAAGAAGTTTTTCAGTGAGCCTGTTGCCAAAATTACATTGCCTTGTAGAGTTCCATATGGATTTCATGGAGCCTTCATGCCAATCTCAACCCCCACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RPE65

Protein Analysis

440

Amino Acids

49.52

Weight (kDa)

5.67

Isoelectric Point (pI)

38.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPE65 PF03055 15 - 165 2.1e-25 Retinal pigment epithelial membrane protein
RPE65 PF03055 171 - 434 9.6e-48 Retinal pigment epithelial membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 280
AciI CCGC 5 cut(s) 270, 280, 294, 580, 960
AclWI GGATC 1 cut(s) 37
AcoI YGGCCR 1 cut(s) 719
AcsI RAATTY 1 cut(s) 731
AfaI GTAC 2 cut(s) 191, 518
AfiI CCNNNNNNNGG 3 cut(s) 59, 490, 560
AflIII ACRYGT 3 cut(s) 202, 216, 1083
AjnI CCWGG 1 cut(s) 685
Alw21I GWGCWC 2 cut(s) 538, 984
Alw26I GTCTC 1 cut(s) 457
Alw44I GTGCAC 1 cut(s) 534
AlwI GGATC 1 cut(s) 37
AoxI GGCC 4 cut(s) 34, 44, 719, 1048
ApaLI GTGCAC 1 cut(s) 534
ApoI RAATTY 1 cut(s) 731
Asp700I GAANNNNTTC 1 cut(s) 1229
AspS9I GGNCC 3 cut(s) 34, 44, 689
AsuHPI GGTGA 2 cut(s) 650, 771
AvaII GGWCC 1 cut(s) 689
AxyI CCTNAGG 2 cut(s) 489, 973
BaeGI GKGCMC 1 cut(s) 538
BalI TGGCCA 1 cut(s) 721
BarI GAAGNNNNNNTAC 4 cut(s) 114, 146, 384, 416
Bbv12I GWGCWC 2 cut(s) 538, 984
BccI CCATC 4 cut(s) 164, 629, 1148, 1152
BceAI ACGGC 3 cut(s) 172, 232, 577
BciT130I CCWGG 1 cut(s) 687
BclI TGATCA 2 cut(s) 763, 897
BcoDI GTCTC 1 cut(s) 457
BfaI CTAG 1 cut(s) 998
BglII AGATCT 1 cut(s) 80
BisI GCNGC 2 cut(s) 271, 960
BlsI GCNGC 2 cut(s) 272, 961
Bme1390I CCNGG 1 cut(s) 687
Bme18I GGWCC 1 cut(s) 689
BmgT120I GGNCC 3 cut(s) 34, 44, 689
BmiI GGNNCC 1 cut(s) 1291
BmrFI CCNGG 1 cut(s) 687
BmrI ACTGGG 1 cut(s) 594
BmsI GCATC 2 cut(s) 540, 556
BmuI ACTGGG 1 cut(s) 594
BpmI CTGGAG 2 cut(s) 477, 1131
BpuEI CTTGAG 2 cut(s) 390, 1185
Bsa29I ATCGAT 1 cut(s) 1215
BsaAI YACGTR 1 cut(s) 106
BsaBI GATNNNNATC 2 cut(s) 70, 416
BsaWI WCCGGW 3 cut(s) 186, 691, 914
Bsc4I CCNNNNNNNGG 3 cut(s) 59, 490, 560
Bse1I ACTGG 3 cut(s) 600, 871, 1114
Bse21I CCTNAGG 2 cut(s) 489, 973
Bse3DI GCAATG 1 cut(s) 1256
Bse8I GATNNNNATC 2 cut(s) 70, 416
BseBI CCWGG 1 cut(s) 687
BseCI ATCGAT 1 cut(s) 1215
BseGI GGATG 3 cut(s) 454, 536, 1163
BseJI GATNNNNATC 2 cut(s) 70, 416
BseLI CCNNNNNNNGG 3 cut(s) 59, 490, 560
BseMI GCAATG 1 cut(s) 1256
BseMII CTCAG 2 cut(s) 690, 987
BseNI ACTGG 3 cut(s) 600, 871, 1114
BseRI GAGGAG 2 cut(s) 293, 1106
BseSI GKGCMC 1 cut(s) 538
Bsh1236I CGCG 1 cut(s) 280
BshFI GGCC 4 cut(s) 36, 46, 721, 1050
BshVI ATCGAT 1 cut(s) 1215
BsiHKAI GWGCWC 2 cut(s) 538, 984
BsiSI CCGG 3 cut(s) 187, 692, 915
BslFI GGGAC 2 cut(s) 167, 1109
BslI CCNNNNNNNGG 3 cut(s) 59, 490, 560
BsmAI GTCTC 1 cut(s) 457
BsmFI GGGAC 2 cut(s) 167, 1109
BsmI GAATGC 1 cut(s) 364
BsnI GGCC 4 cut(s) 36, 46, 721, 1050
Bsp1286I GDGCHC 2 cut(s) 538, 984
Bsp143I GATC 6 cut(s) 29, 80, 394, 763, 897, 1212
BspACI CCGC 5 cut(s) 270, 280, 294, 580, 960
BspANI GGCC 4 cut(s) 36, 46, 721, 1050
BspCNI CTCAG 2 cut(s) 689, 986
BspDI ATCGAT 1 cut(s) 1215
BspFNI CGCG 1 cut(s) 280
BspHI TCATGA 2 cut(s) 523, 766
BspLI GGNNCC 1 cut(s) 1291
BspPI GGATC 1 cut(s) 37
BsrDI GCAATG 1 cut(s) 1256
BsrI ACTGG 3 cut(s) 600, 871, 1114
BssMI GATC 6 cut(s) 29, 80, 394, 763, 897, 1212
Bst2UI CCWGG 1 cut(s) 687
Bst4CI ACNGT 1 cut(s) 184
Bst6I CTCTTC 2 cut(s) 636, 1053
BstAPI GCANNNNNTGC 1 cut(s) 968
BstBAI YACGTR 1 cut(s) 106
BstC8I GCNNGC 4 cut(s) 128, 282, 964, 980
BstDEI CTNAG 4 cut(s) 489, 676, 973, 1317
BstF5I GGATG 3 cut(s) 454, 536, 1163
BstFNI CGCG 1 cut(s) 280
BstKTI GATC 6 cut(s) 32, 83, 397, 766, 900, 1215
BstMAI GTCTC 1 cut(s) 457
BstMBI GATC 6 cut(s) 29, 80, 394, 763, 897, 1212
BstMWI GCNNNNNNNGC 2 cut(s) 244, 968
BstNI CCWGG 1 cut(s) 687
BstNSI RCATGY 4 cut(s) 130, 137, 835, 1087
BstSCI CCNGG 1 cut(s) 685
BstSLI GKGCMC 1 cut(s) 538
BstUI CGCG 1 cut(s) 280
BstX2I RGATCY 1 cut(s) 80
BstYI RGATCY 1 cut(s) 80
Bsu15I ATCGAT 1 cut(s) 1215
Bsu36I CCTNAGG 2 cut(s) 489, 973
BsuRI GGCC 4 cut(s) 36, 46, 721, 1050
BsuTUI ATCGAT 1 cut(s) 1215
BtgZI GCGATG 1 cut(s) 579
BtsCI GGATG 3 cut(s) 454, 536, 1163
BtsIMutI CAGTG 4 cut(s) 856, 864, 1107, 1240
Cac8I GCNNGC 4 cut(s) 128, 282, 964, 980
CciI TCATGA 2 cut(s) 523, 766
Cfr13I GGNCC 3 cut(s) 34, 44, 689
ClaI ATCGAT 1 cut(s) 1215
Csp6I GTAC 2 cut(s) 190, 517
CspCI CAANNNNNGTGG 4 cut(s) 698, 733, 1053, 1088
CviQI GTAC 2 cut(s) 190, 517
DdeI CTNAG 4 cut(s) 489, 676, 973, 1317
DpnI GATC 6 cut(s) 31, 82, 396, 765, 899, 1214
DpnII GATC 6 cut(s) 29, 80, 394, 763, 897, 1212
DraI TTTAAA 1 cut(s) 589
EaeI YGGCCR 1 cut(s) 719
Eam1104I CTCTTC 2 cut(s) 636, 1053
EarI CTCTTC 2 cut(s) 636, 1053
Eco32I GATATC 1 cut(s) 860
Eco47I GGWCC 1 cut(s) 689
Eco81I CCTNAGG 2 cut(s) 489, 973
EcoRII CCWGG 1 cut(s) 685
EcoRV GATATC 1 cut(s) 860
FalI AAGNNNNNCTT 2 cut(s) 995, 1027
FaqI GGGAC 2 cut(s) 167, 1109
FauI CCCGC 1 cut(s) 273
FauNDI CATATG 1 cut(s) 1276
FbaI TGATCA 2 cut(s) 763, 897
Fnu4HI GCNGC 2 cut(s) 271, 960
FokI GGATG 3 cut(s) 461, 543, 1170
Fsp4HI GCNGC 2 cut(s) 271, 960
FspBI CTAG 1 cut(s) 998
GluI GCNGC 2 cut(s) 271, 960
GsuI CTGGAG 2 cut(s) 477, 1131
HaeIII GGCC 4 cut(s) 36, 46, 721, 1050
HapII CCGG 3 cut(s) 187, 692, 915
HindIII AAGCTT 1 cut(s) 1203
HinfI GANTC 6 cut(s) 275, 540, 572, 674, 706, 953
HpaII CCGG 3 cut(s) 187, 692, 915
HphI GGTGA 2 cut(s) 650, 771
Hpy166II GTNNAC 2 cut(s) 536, 1177
Hpy188I TCNGA 1 cut(s) 923
Hpy188III TCNNGA 6 cut(s) 407, 456, 524, 671, 767, 1142
Hpy8I GTNNAC 2 cut(s) 536, 1177
Hpy99I CGWCG 1 cut(s) 164
HpyAV CCTTC 6 cut(s) 87, 110, 116, 521, 1040, 1303
HpyCH4III ACNGT 1 cut(s) 184
HpyCH4IV ACGT 4 cut(s) 105, 162, 204, 218
HpyCH4V TGCA 7 cut(s) 504, 536, 569, 606, 835, 882, 1107
HpyF10VI GCNNNNNNNGC 2 cut(s) 244, 968
HpyF3I CTNAG 4 cut(s) 489, 676, 973, 1317
HpySE526I ACGT 4 cut(s) 105, 162, 204, 218
Ksp22I TGATCA 2 cut(s) 763, 897
Kzo9I GATC 6 cut(s) 29, 80, 394, 763, 897, 1212
LmnI GCTCC 4 cut(s) 458, 905, 1112, 1289
LweI GCATC 2 cut(s) 540, 556
MaeI CTAG 1 cut(s) 998
MaeII ACGT 4 cut(s) 105, 162, 204, 218
MaeIII GTNAC 1 cut(s) 101
MalI GATC 6 cut(s) 31, 82, 396, 765, 899, 1214
MboI GATC 6 cut(s) 29, 80, 394, 763, 897, 1212
MboII GAAGA 8 cut(s) 404, 644, 653, 1028, 1070, 1157, 1160, 1196
MfeI CAATTG 1 cut(s) 442
MflI RGATCY 1 cut(s) 80
MhlI GDGCHC 2 cut(s) 538, 984
MlsI TGGCCA 1 cut(s) 721
MluCI AATT 9 cut(s) 19, 39, 307, 442, 710, 731, 744, 1052, 1251
MluNI TGGCCA 1 cut(s) 721
MlyI GAGTC 2 cut(s) 269, 668
Mox20I TGGCCA 1 cut(s) 721
MroXI GAANNNNTTC 1 cut(s) 1229
MscI TGGCCA 1 cut(s) 721
MseI TTAA 3 cut(s) 588, 735, 827
MslI CAYNNNNRTG 1 cut(s) 813
Msp20I TGGCCA 1 cut(s) 721
MspA1I CMGCKG 2 cut(s) 582, 1025
MspI CCGG 3 cut(s) 187, 692, 915
MspR9I CCNGG 1 cut(s) 687
MunI CAATTG 1 cut(s) 442
Mva1269I GAATGC 1 cut(s) 364
MvaI CCWGG 1 cut(s) 687
MvnI CGCG 1 cut(s) 280
MwoI GCNNNNNNNGC 2 cut(s) 244, 968
NdeI CATATG 1 cut(s) 1276
NdeII GATC 6 cut(s) 29, 80, 394, 763, 897, 1212
NlaIV GGNNCC 1 cut(s) 1291
NmuCI GTSAC 1 cut(s) 101
NspI RCATGY 4 cut(s) 130, 137, 835, 1087
PaeI GCATGC 1 cut(s) 130
PagI TCATGA 2 cut(s) 523, 766
PciI ACATGT 1 cut(s) 1083
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PctI GAATGC 1 cut(s) 364
PdmI GAANNNNTTC 1 cut(s) 1229
PfeI GAWTC 4 cut(s) 540, 572, 706, 953
PkrI GCNGC 2 cut(s) 272, 961
PleI GAGTC 2 cut(s) 269, 668
PpsI GAGTC 2 cut(s) 269, 668
Ppu21I YACGTR 1 cut(s) 106
PscI ACATGT 1 cut(s) 1083
Psp6I CCWGG 1 cut(s) 685
PspGI CCWGG 1 cut(s) 685
PspN4I GGNNCC 1 cut(s) 1291
PspPI GGNCC 3 cut(s) 34, 44, 689
PsuI RGATCY 1 cut(s) 80
PvuII CAGCTG 1 cut(s) 1025
RsaI GTAC 2 cut(s) 191, 518
RsaNI GTAC 2 cut(s) 190, 517
RseI CAYNNNNRTG 1 cut(s) 813
SaqAI TTAA 3 cut(s) 588, 735, 827
SatI GCNGC 2 cut(s) 271, 960
Sau3AI GATC 6 cut(s) 29, 80, 394, 763, 897, 1212
Sau96I GGNCC 3 cut(s) 34, 44, 689
SchI GAGTC 2 cut(s) 269, 668
ScrFI CCNGG 1 cut(s) 687
SduI GDGCHC 2 cut(s) 538, 984
SfaNI GCATC 2 cut(s) 540, 556
SinI GGWCC 1 cut(s) 689
SmiMI CAYNNNNRTG 1 cut(s) 813
SmlI CTYRAG 2 cut(s) 405, 1200
SmoI CTYRAG 2 cut(s) 405, 1200
SphI GCATGC 1 cut(s) 130
Sse9I AATT 9 cut(s) 19, 39, 307, 442, 710, 731, 744, 1052, 1251
SsiI CCGC 5 cut(s) 270, 280, 294, 580, 960
SspMI CTAG 1 cut(s) 998
StyD4I CCNGG 1 cut(s) 685
TaaI ACNGT 1 cut(s) 184
TaiI ACGT 4 cut(s) 108, 165, 207, 221
TaqI TCGA 5 cut(s) 74, 228, 360, 786, 1215
TasI AATT 9 cut(s) 19, 39, 307, 442, 710, 731, 744, 1052, 1251
TatI WGTACW 1 cut(s) 516
TauI GCSGC 2 cut(s) 273, 962
TfiI GAWTC 4 cut(s) 540, 572, 706, 953
Tru1I TTAA 3 cut(s) 588, 735, 827
Tru9I TTAA 3 cut(s) 588, 735, 827
TscAI CASTG 4 cut(s) 856, 871, 1114, 1240
TseFI GTSAC 1 cut(s) 101
Tsp45I GTSAC 1 cut(s) 101
TspDTI ATGAA 6 cut(s) 540, 807, 831, 1197, 1274, 1285
TspGWI ACGGA 1 cut(s) 918
TspRI CASTG 4 cut(s) 856, 871, 1114, 1240
VneI GTGCAC 1 cut(s) 534
VpaK11BI GGWCC 1 cut(s) 689
XapI RAATTY 1 cut(s) 731
XceI RCATGY 4 cut(s) 130, 137, 835, 1087
XmnI GAANNNNTTC 1 cut(s) 1229
XspI CTAG 1 cut(s) 998
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.