Rw6G040050

Carotenoid 9,10(9',10')-cleavage dioxygenase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0013626
Physical Location & Seq
Reverse (-)
63888427 .. 63894249
5823 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G040050.1

Sequence Viewer

Length: 1860 bp
ATGAAGACGCAGAGAGAATTGGCGACTTCATGTTCATCAAGTATGGCTGTGGCGTTTCAAGTGAATTGCTGTATTCAGAACAAGAAGCCCCCAGCTGTCCTAGACAATATTGAGTACTTCAAGACCACTCTCTCCTCGGCTATCATGAAGCAGCCATTGTTGAGAAGGATGCAACAAGTTTCCATGCATATAGATGTCTCGAAAATCTTCAAGAACGCTTCTGCAAAAATGTTGGACGCTTTTGTAGATTCGGTGTTTGAATTCATTGATCAACCATTGCTTCCATCTCAGAAGAACTTCGCCCCAGTTGGTGAATTGGAAGGAGAAGGAGTTGTAATCACGAGCATCATCGGAAGAATTCCAGATAACTTTCCGGAGGGAGTCTACGTAAGAAATGGACCAAACCCTTTATTTGGAGGATTGAAATCGACGAAATCAGTGTTTGGAAGGTCAAGTCATATATGGATAGAAGGAGAAGGCATGGTTCATGGTCTGTATTTTACGAAGAACGACGTCGTCAGCAGTGATGGTAGCAGATGGACGGTCCGGTACAACAACAGACATGTCGAAACCGAAACTTACAAGCTTGAAAAACAAAGAAACAAGCCTTCTTTTCTCCCTGCCATTGGTGGCGACTCGCCGGCTATTTTGTCTGCTTATCTCCTCAATTTGTTGCGGTTTGGCAAAGTAAACAAGTACTTAAGCAACACAAATGTGTTTGAGCATTCGGGGAGGTTCTACTCAGTTGCAGAAAATCACATACCACAAGAGATTGATATCCTTACGCTGGAAACTTTAGGCAATTGGGATGTCTCTGGAGCTTGGAAAAGACCTTTTATCAGCCACCCGAAGAGAGCTCCAGGCACAGGAGAGTTGGTCATAATGGGCGTGGACGCAGTTAAACCGTTTTGGGAAGTTGGGGTAGTTTCTGCTGATGGCAAAGAATTAATCCACAAGGTGGATCTCAAACTCAATAGGTGCAGCATTTGCCATGAAATAGGGGTTACGCAGAGGTACAATGTGATCATGGATATTGCACTAACTATAGACATAAAGAGACTCGTAGGTGGAGGCCCATTAGTAAAGTATAATGAGGAAGAATATGCAAGAATTGGTGTAATGCCTCGCTACGGTGACGCAAGTTCAATCCGGTGGTTTAAAGTTGAACCCAATTGCACATTTCACATCATCAATAGTTTTGAAGCTGGTGATGAGGTTGTAGTGTGGGGTTGTAAAGCTCTTGACTCAGTTATAGGTCCAAGTAATGAAAGCAGTACCGATAATAGCACCTCAACGGCAGAGGATGCTAGTAAATTATACTCCCATGCTTATGAATGGAGATTAAACATGGAGACTGGAGAAGTCAGGGAGAGATATCTAACTGGCAAAAAGTTTTCCATGGATTTTCCTACGATCAATGGAAACTTTTGCGGCCTTAAAAACAGATTTGGGTATGCCCAAGTCGTCGATTCAGTTGCGAGCTCTACCTCAGGGTTGCTTAAGTATGGTGGTATAGCTAAGCTACATTTTGAAGAGCCAGCTGAGAGAGAAGGCGAATTAGAAAATGAGGAAATAAAGGTAGAAAGACATATGTTTGAGGAGAACACCTTTTGTACCGGAGCTGCTTTTGTCCCTAAACAAAGTGATAGTAGCAGGACTGGTAGTATTATTCTTGAAGAAGTAGAAGAAGATGATGGATGGGTTATTAGTTTTGTTCACAATGAAGATACCAAAGTATCACAAGTTTATGTTATTGATACCAAGGAGTTTTGCAGTGAGCCTGTAGCCAAAATTACATTGCCCTGCAGAGCTCCATATGGATTTCATGGAGCTTTCATGCCAACCTCATCGAACACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RPE65

Protein Analysis

619

Amino Acids

69.07

Weight (kDa)

5.77

Isoelectric Point (pI)

42.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPE65 PF03055 106 - 614 5.7e-110 Retinal pigment epithelial membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 516
AccB7I CCANNNNNTGG 1 cut(s) 958
AccI GTMKAC 1 cut(s) 384
AccIII TCCGGA 1 cut(s) 373
AciI CCGC 2 cut(s) 676, 1431
AclWI GGATC 1 cut(s) 969
AcsI RAATTY 2 cut(s) 260, 357
AcyI GRCGYC 1 cut(s) 513
AdeI CACNNNGTG 1 cut(s) 958
AfaI GTAC 6 cut(s) 116, 551, 698, 1016, 1276, 1615
AfiI CCNNNNNNNGG 6 cut(s) 413, 626, 787, 866, 958, 1130
AflII CTTAAG 2 cut(s) 700, 1499
AflIII ACRYGT 1 cut(s) 562
AjnI CCWGG 1 cut(s) 859
AleI CACNNNNGTG 1 cut(s) 713
Alw21I GWGCWC 3 cut(s) 859, 1484, 1813
Alw26I GTCTC 4 cut(s) 202, 817, 1051, 1346
AlwI GGATC 1 cut(s) 969
Aor13HI TCCGGA 1 cut(s) 373
AoxI GGCC 2 cut(s) 1072, 1432
ApeKI GCWGC 3 cut(s) 151, 981, 1622
ApoI RAATTY 2 cut(s) 260, 357
AseI ATTAAT 1 cut(s) 947
Asp700I GAANNNNTTC 2 cut(s) 206, 296
AspS9I GGNCC 4 cut(s) 398, 544, 1073, 1256
AsuHPI GGTGA 3 cut(s) 323, 1145, 1220
AvaII GGWCC 3 cut(s) 398, 544, 1256
AxyI CCTNAGG 1 cut(s) 1489
BaeI ACNNNNGTAYC 4 cut(s) 1719, 1719, 1752, 1752
BanII GRGCYC 3 cut(s) 859, 1484, 1813
BauI CACGAG 1 cut(s) 340
BbsI GAAGAC 1 cut(s) 11
Bbv12I GWGCWC 3 cut(s) 859, 1484, 1813
BbvI GCAGC 3 cut(s) 163, 993, 1609
BccI CCATC 6 cut(s) 292, 521, 531, 929, 1688, 1692
BceAI ACGGC 1 cut(s) 1311
BcgI CGANNNNNNTGC 2 cut(s) 1457, 1491
BciT130I CCWGG 1 cut(s) 861
BclI TGATCA 2 cut(s) 268, 1023
BcoDI GTCTC 4 cut(s) 202, 817, 1051, 1346
BfaI CTAG 2 cut(s) 101, 1308
BfmI CTRYAG 3 cut(s) 1044, 1782, 1804
BfrI CTTAAG 2 cut(s) 700, 1499
BisI GCNGC 4 cut(s) 152, 982, 1432, 1623
BlpI GCTNAGC 1 cut(s) 1518
BlsI GCNGC 4 cut(s) 153, 983, 1433, 1624
BmcAI AGTACT 2 cut(s) 116, 698
Bme1390I CCNGG 1 cut(s) 861
Bme18I GGWCC 3 cut(s) 398, 544, 1256
BmgT120I GGNCC 4 cut(s) 398, 544, 1073, 1256
BmrFI CCNGG 1 cut(s) 861
BmrI ACTGGG 1 cut(s) 299
BmsI GCATC 3 cut(s) 159, 354, 1294
BmuI ACTGGG 1 cut(s) 299
BpiI GAAGAC 1 cut(s) 11
BpmI CTGGAG 3 cut(s) 837, 843, 1377
Bpu1102I GCTNAGC 1 cut(s) 1518
BsaAI YACGTR 1 cut(s) 388
BsaBI GATNNNNATC 2 cut(s) 424, 776
BsaHI GRCGYC 1 cut(s) 513
BsaJI CCNNGG 3 cut(s) 135, 1398, 1761
BsaWI WCCGGW 4 cut(s) 373, 546, 1149, 1616
BsaXI ACNNNNNCTCC 6 cut(s) 315, 345, 372, 402, 861, 891
Bsc4I CCNNNNNNNGG 6 cut(s) 413, 626, 787, 866, 958, 1130
Bse118I RCCGGY 1 cut(s) 640
Bse1I ACTGG 4 cut(s) 305, 1360, 1387, 1663
Bse21I CCTNAGG 1 cut(s) 1489
Bse3DI GCAATG 2 cut(s) 275, 1796
Bse8I GATNNNNATC 2 cut(s) 424, 776
BseAI TCCGGA 1 cut(s) 373
BseBI CCWGG 1 cut(s) 861
BseDI CCNNGG 3 cut(s) 135, 1398, 1761
BseGI GGATG 4 cut(s) 174, 814, 1309, 1703
BseJI GATNNNNATC 2 cut(s) 424, 776
BseLI CCNNNNNNNGG 6 cut(s) 413, 626, 787, 866, 958, 1130
BseMI GCAATG 2 cut(s) 275, 1796
BseMII CTCAG 5 cut(s) 302, 756, 1260, 1503, 1533
BseNI ACTGG 4 cut(s) 305, 1360, 1387, 1663
BseRI GAGGAG 3 cut(s) 124, 653, 1613
BseXI GCAGC 3 cut(s) 163, 993, 1609
BseYI CCCAGC 1 cut(s) 91
BsgI GTGCAG 1 cut(s) 1000
BshFI GGCC 2 cut(s) 1074, 1434
BsiHKAI GWGCWC 3 cut(s) 859, 1484, 1813
BsiSI CCGG 5 cut(s) 374, 547, 641, 1150, 1617
BslFI GGGAC 1 cut(s) 1616
BslI CCNNNNNNNGG 6 cut(s) 413, 626, 787, 866, 958, 1130
BsmAI GTCTC 4 cut(s) 202, 817, 1051, 1346
BsmFI GGGAC 1 cut(s) 1616
BsmI GAATGC 1 cut(s) 724
BsnI GGCC 2 cut(s) 1074, 1434
Bsp1286I GDGCHC 3 cut(s) 859, 1484, 1813
Bsp13I TCCGGA 1 cut(s) 373
Bsp143I GATC 4 cut(s) 268, 961, 1023, 1413
Bsp1720I GCTNAGC 1 cut(s) 1518
Bsp19I CCATGG 1 cut(s) 1398
BspACI CCGC 2 cut(s) 676, 1431
BspANI GGCC 2 cut(s) 1074, 1434
BspCNI CTCAG 5 cut(s) 301, 755, 1259, 1502, 1534
BspEI TCCGGA 1 cut(s) 373
BspHI TCATGA 1 cut(s) 144
BspMAI CTGCAG 1 cut(s) 1808
BspPI GGATC 1 cut(s) 969
BspQI GCTCTTC 1 cut(s) 1527
BspTI CTTAAG 2 cut(s) 700, 1499
BsrDI GCAATG 2 cut(s) 275, 1796
BsrFI RCCGGY 1 cut(s) 640
BsrI ACTGG 4 cut(s) 305, 1360, 1387, 1663
BssAI RCCGGY 1 cut(s) 640
BssECI CCNNGG 3 cut(s) 135, 1398, 1761
BssMI GATC 4 cut(s) 268, 961, 1023, 1413
BssNI GRCGYC 1 cut(s) 513
BssSI CACGAG 1 cut(s) 340
BssT1I CCWWGG 2 cut(s) 1398, 1761
Bst2BI CACGAG 1 cut(s) 340
Bst2UI CCWGG 1 cut(s) 861
Bst4CI ACNGT 3 cut(s) 544, 906, 1133
Bst6I CTCTTC 2 cut(s) 845, 1527
BstACI GRCGYC 1 cut(s) 513
BstAFI CTTAAG 2 cut(s) 700, 1499
BstAPI GCANNNNNTGC 2 cut(s) 987, 1304
BstBAI YACGTR 1 cut(s) 388
BstC8I GCNNGC 3 cut(s) 642, 1480, 1539
BstDEI CTNAG 6 cut(s) 288, 742, 1246, 1489, 1518, 1542
BstDSI CCRYGG 1 cut(s) 1398
BstF5I GGATG 4 cut(s) 174, 814, 1309, 1703
BstKTI GATC 4 cut(s) 271, 964, 1026, 1416
BstMAI GTCTC 4 cut(s) 202, 817, 1051, 1346
BstMBI GATC 4 cut(s) 268, 961, 1023, 1413
BstMWI GCNNNNNNNGC 2 cut(s) 987, 1304
BstNI CCWGG 1 cut(s) 861
BstNSI RCATGY 1 cut(s) 566
BstSCI CCNGG 1 cut(s) 859
BstSFI CTRYAG 3 cut(s) 1044, 1782, 1804
BstSNI TACGTA 1 cut(s) 388
BstV1I GCAGC 3 cut(s) 163, 993, 1609
BstV2I GAAGAC 1 cut(s) 11
BstX2I RGATCY 1 cut(s) 961
BstYI RGATCY 1 cut(s) 961
Bsu36I CCTNAGG 1 cut(s) 1489
BsuRI GGCC 2 cut(s) 1074, 1434
BtgI CCRYGG 1 cut(s) 1398
BtsCI GGATG 4 cut(s) 174, 814, 1309, 1703
BtsI GCAGTG 2 cut(s) 529, 1780
BtsIMutI CAGTG 3 cut(s) 444, 529, 1780
Cac8I GCNNGC 3 cut(s) 642, 1480, 1539
CciI TCATGA 1 cut(s) 144
Cfr10I RCCGGY 1 cut(s) 640
Cfr13I GGNCC 4 cut(s) 398, 544, 1073, 1256
CpoI CGGWCCG 1 cut(s) 544
CseI GACGC 4 cut(s) 16, 245, 902, 1145
Csp6I GTAC 6 cut(s) 115, 550, 697, 1015, 1275, 1614
CspI CGGWCCG 1 cut(s) 544
CviQI GTAC 6 cut(s) 115, 550, 697, 1015, 1275, 1614
DdeI CTNAG 6 cut(s) 288, 742, 1246, 1489, 1518, 1542
DpnI GATC 4 cut(s) 270, 963, 1025, 1415
DpnII GATC 4 cut(s) 268, 961, 1023, 1413
DraI TTTAAA 1 cut(s) 1159
DraIII CACNNNGTG 1 cut(s) 958
Eam1104I CTCTTC 2 cut(s) 845, 1527
EarI CTCTTC 2 cut(s) 845, 1527
Ecl136II GAGCTC 3 cut(s) 857, 1482, 1811
Eco105I TACGTA 1 cut(s) 388
Eco130I CCWWGG 2 cut(s) 1398, 1761
Eco24I GRGCYC 3 cut(s) 859, 1484, 1813
Eco32I GATATC 2 cut(s) 778, 1376
Eco47I GGWCC 3 cut(s) 398, 544, 1256
Eco53kI GAGCTC 3 cut(s) 857, 1482, 1811
Eco81I CCTNAGG 1 cut(s) 1489
EcoICRI GAGCTC 3 cut(s) 857, 1482, 1811
EcoRI GAATTC 2 cut(s) 260, 357
EcoRII CCWGG 1 cut(s) 859
EcoRV GATATC 2 cut(s) 778, 1376
EcoT14I CCWWGG 2 cut(s) 1398, 1761
EcoT22I ATGCAT 1 cut(s) 189
EcoT38I GRGCYC 3 cut(s) 859, 1484, 1813
ErhI CCWWGG 2 cut(s) 1398, 1761
FaqI GGGAC 1 cut(s) 1616
FauNDI CATATG 2 cut(s) 1590, 1816
FbaI TGATCA 2 cut(s) 268, 1023
FblI GTMKAC 1 cut(s) 384
Fnu4HI GCNGC 4 cut(s) 152, 982, 1432, 1623
FokI GGATG 4 cut(s) 181, 821, 1316, 1710
FriOI GRGCYC 3 cut(s) 859, 1484, 1813
Fsp4HI GCNGC 4 cut(s) 152, 982, 1432, 1623
FspBI CTAG 2 cut(s) 101, 1308
GluI GCNGC 4 cut(s) 152, 982, 1432, 1623
GsaI CCCAGC 1 cut(s) 95
GsuI CTGGAG 3 cut(s) 837, 843, 1377
HaeIII GGCC 2 cut(s) 1074, 1434
HapII CCGG 5 cut(s) 374, 547, 641, 1150, 1617
HgaI GACGC 4 cut(s) 16, 245, 902, 1145
Hin1I GRCGYC 1 cut(s) 513
HindIII AAGCTT 1 cut(s) 584
HinfI GANTC 6 cut(s) 248, 381, 635, 1059, 1244, 1469
HpaII CCGG 5 cut(s) 374, 547, 641, 1150, 1617
HphI GGTGA 3 cut(s) 323, 1145, 1220
Hpy166II GTNNAC 4 cut(s) 385, 691, 892, 1717
Hpy188I TCNGA 3 cut(s) 78, 291, 353
Hpy8I GTNNAC 4 cut(s) 385, 691, 892, 1717
Hpy99I CGWCG 4 cut(s) 433, 515, 518, 1469
HpyAV CCTTC 8 cut(s) 159, 314, 320, 441, 464, 470, 618, 1544
HpyCH4III ACNGT 3 cut(s) 544, 906, 1133
HpyCH4IV ACGT 2 cut(s) 387, 513
HpyF10VI GCNNNNNNNGC 2 cut(s) 987, 1304
HpyF3I CTNAG 6 cut(s) 288, 742, 1246, 1489, 1518, 1542
HpySE526I ACGT 2 cut(s) 387, 513
Hsp92I GRCGYC 1 cut(s) 513
Kpn2I TCCGGA 1 cut(s) 373
KroI GCCGGC 1 cut(s) 640
KroNI GCCGGC 1 cut(s) 642
Ksp22I TGATCA 2 cut(s) 268, 1023
Kzo9I GATC 4 cut(s) 268, 961, 1023, 1413
LguI GCTCTTC 1 cut(s) 1527
LmnI GCTCC 5 cut(s) 818, 862, 1619, 1816, 1829
Lsp1109I GCAGC 3 cut(s) 163, 993, 1609
LweI GCATC 3 cut(s) 159, 354, 1294
MaeI CTAG 2 cut(s) 101, 1308
MaeII ACGT 2 cut(s) 387, 513
MaeIII GTNAC 2 cut(s) 1003, 1133
MalI GATC 4 cut(s) 270, 963, 1025, 1415
MboI GATC 4 cut(s) 268, 961, 1023, 1413
MfeI CAATTG 2 cut(s) 802, 1171
MflI RGATCY 1 cut(s) 961
MhlI GDGCHC 3 cut(s) 859, 1484, 1813
MlyI GAGTC 4 cut(s) 390, 629, 1053, 1238
MmeI TCCRAC 1 cut(s) 213
Mph1103I ATGCAT 1 cut(s) 189
MroI TCCGGA 1 cut(s) 373
MroNI GCCGGC 1 cut(s) 640
MroXI GAANNNNTTC 2 cut(s) 206, 296
MseI TTAA 7 cut(s) 701, 900, 947, 1158, 1343, 1437, 1500
MslI CAYNNNNRTG 3 cut(s) 192, 713, 1329
MspA1I CMGCKG 2 cut(s) 95, 1541
MspCI CTTAAG 2 cut(s) 700, 1499
MspI CCGG 5 cut(s) 374, 547, 641, 1150, 1617
MspR9I CCNGG 1 cut(s) 861
MunI CAATTG 2 cut(s) 802, 1171
Mva1269I GAATGC 1 cut(s) 724
MvaI CCWGG 1 cut(s) 861
MwoI GCNNNNNNNGC 2 cut(s) 987, 1304
NaeI GCCGGC 1 cut(s) 642
NcoI CCATGG 1 cut(s) 1398
NdeI CATATG 2 cut(s) 1590, 1816
NdeII GATC 4 cut(s) 268, 961, 1023, 1413
NgoMIV GCCGGC 1 cut(s) 640
NmeAIII GCCGAG 1 cut(s) 116
NmuCI GTSAC 1 cut(s) 1133
NsiI ATGCAT 1 cut(s) 189
NspI RCATGY 1 cut(s) 566
OliI CACNNNNGTG 1 cut(s) 713
PagI TCATGA 1 cut(s) 144
PciI ACATGT 1 cut(s) 562
PciSI GCTCTTC 1 cut(s) 1527
PctI GAATGC 1 cut(s) 724
PdiI GCCGGC 1 cut(s) 642
PdmI GAANNNNTTC 2 cut(s) 206, 296
PfeI GAWTC 2 cut(s) 248, 1469
PflFI GACNNNGTC 1 cut(s) 515
PflMI CCANNNNNTGG 1 cut(s) 958
PkrI GCNGC 4 cut(s) 153, 983, 1433, 1624
PleI GAGTC 4 cut(s) 389, 629, 1053, 1238
PpsI GAGTC 4 cut(s) 389, 629, 1053, 1238
Ppu21I YACGTR 1 cut(s) 388
PscI ACATGT 1 cut(s) 562
PshBI ATTAAT 1 cut(s) 947
Psp124BI GAGCTC 3 cut(s) 859, 1484, 1813
Psp6I CCWGG 1 cut(s) 859
PspFI CCCAGC 1 cut(s) 91
PspGI CCWGG 1 cut(s) 859
PspPI GGNCC 4 cut(s) 398, 544, 1073, 1256
PstI CTGCAG 1 cut(s) 1808
PsuI RGATCY 1 cut(s) 961
PsyI GACNNNGTC 1 cut(s) 515
PvuII CAGCTG 2 cut(s) 95, 1541
RsaI GTAC 6 cut(s) 116, 551, 698, 1016, 1276, 1615
RsaNI GTAC 6 cut(s) 115, 550, 697, 1015, 1275, 1614
RseI CAYNNNNRTG 3 cut(s) 192, 713, 1329
Rsr2I CGGWCCG 1 cut(s) 544
RsrII CGGWCCG 1 cut(s) 544
SacI GAGCTC 3 cut(s) 859, 1484, 1813
SapI GCTCTTC 1 cut(s) 1527
SaqAI TTAA 7 cut(s) 701, 900, 947, 1158, 1343, 1437, 1500
SatI GCNGC 4 cut(s) 152, 982, 1432, 1623
Sau3AI GATC 4 cut(s) 268, 961, 1023, 1413
Sau96I GGNCC 4 cut(s) 398, 544, 1073, 1256
ScaI AGTACT 2 cut(s) 116, 698
SchI GAGTC 4 cut(s) 390, 629, 1053, 1238
ScrFI CCNGG 1 cut(s) 861
SduI GDGCHC 3 cut(s) 859, 1484, 1813
SfaNI GCATC 3 cut(s) 159, 354, 1294
SfcI CTRYAG 3 cut(s) 1044, 1782, 1804
SinI GGWCC 3 cut(s) 398, 544, 1256
SmiMI CAYNNNNRTG 3 cut(s) 192, 713, 1329
SmlI CTYRAG 2 cut(s) 700, 1499
SmoI CTYRAG 2 cut(s) 700, 1499
SnaBI TACGTA 1 cut(s) 388
SsiI CCGC 2 cut(s) 676, 1431
SspI AATATT 1 cut(s) 109
SspMI CTAG 2 cut(s) 101, 1308
SstI GAGCTC 3 cut(s) 859, 1484, 1813
StyD4I CCNGG 1 cut(s) 859
StyI CCWWGG 2 cut(s) 1398, 1761
TaaI ACNGT 3 cut(s) 544, 906, 1133
TaiI ACGT 2 cut(s) 390, 516
TaqI TCGA 5 cut(s) 200, 428, 567, 1467, 1850
TatI WGTACW 2 cut(s) 114, 696
TauI GCSGC 1 cut(s) 1434
TfiI GAWTC 2 cut(s) 248, 1469
Tru1I TTAA 7 cut(s) 701, 900, 947, 1158, 1343, 1437, 1500
Tru9I TTAA 7 cut(s) 701, 900, 947, 1158, 1343, 1437, 1500
TscAI CASTG 3 cut(s) 444, 529, 1780
TseFI GTSAC 1 cut(s) 1133
TseI GCWGC 3 cut(s) 151, 981, 1622
Tsp45I GTSAC 1 cut(s) 1133
TspRI CASTG 3 cut(s) 444, 529, 1780
Tth111I GACNNNGTC 1 cut(s) 515
Van91I CCANNNNNTGG 1 cut(s) 958
Vha464I CTTAAG 2 cut(s) 700, 1499
VpaK11BI GGWCC 3 cut(s) 398, 544, 1256
VspI ATTAAT 1 cut(s) 947
XapI RAATTY 2 cut(s) 260, 357
XceI RCATGY 1 cut(s) 566
XmiI GTMKAC 1 cut(s) 384
XmnI GAANNNNTTC 2 cut(s) 206, 296
XspI CTAG 2 cut(s) 101, 1308
ZraI GACGTC 1 cut(s) 514
ZrmI AGTACT 2 cut(s) 116, 698
Zsp2I ATGCAT 1 cut(s) 189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.