Rh6DG460200

Carotenoid 9,10(9',10')-cleavage dioxygenase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0013626
Physical Location & Seq
Reverse (-)
62967504 .. 62971509
4006 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG460200.1

Sequence Viewer

Length: 1818 bp
ATGGGTATGGCGTTTCAAGTGAATTGCTGTATTCAGAACAAGAAGCCCTCAGCTGTCCTAGACAATGTTGAGTACTTCAAGACCACACTCTCCTCGGCTATCATGAATCAGCCATTGTTGAGAAGGATGCAACAAGTTCCCATGCATATAGATGTCTCGAAAATCTTCAAGAACGCTTCTGCAAAAATGTTGGACGCCTTTGTAGATTCGGTGTTTGAATTCATTGATCAACCATTGCTTCCATCTCAGAAGAACTTCGCCCCAGTTGGTGAATTGGAAGGAGAAGGAGTTGTAATCACGAGCATCATCGGAAGAATTCCAGATAACTTTCCGGAGGGAGTCTACGTAAGAAATGGACCAAACCCTTTATTTGGAGGATTGAAATCGACGAAATCGGTGTTTGGAAGGTCAAGTCATATATGGATAGAAGGAGAAGGCATGGTTCATGCTTTGTATTTTACGAAGAACGACGTCGTCAGCAGTGATGGTAGCAGATGGACGGTCCGGTACAACAACAGACATGTCGAAACCGAAACTTACAAGCTTGAAAAACAAAGAAACAAGCCTTCTTTTCTCCCTGCCATTGGTGGCGACTCGCCGGCTATTTTGTCTGCTTATCTCCTCAATTTGTTGCGGTTTGGCAAAGTAAACAAGTACTTAAGCAACACAAATGTGTTTGAGCATTCGGGGAGGTTTTACTCAGTTGCAGAAAATCACATACCACAAGAGATTGATATCCTTACGCTGGAAACTTTAGGCAATTGGGATGTCTCTGGAGCTTGGAACAGACCTTTTATCAGCCACCCGAAGAGAGCTCCAGGCACAGGAGAGTTGGTCATACTGGGCGTGGACGCAGTTAAACCGTTTTGGGAAGTAGGGGTAGTTTCTGCTGATGGCAAAGAATTAATCCACAAGGTGGATCTCAAACTCAATAGGTGCAGCATTTGCCATGAAATAGGGGTTACGCAGAGATACAATGTGATCATGGATATTGCACTAACTATAGACATAAAGAGACTCGTAGGTGGAGGCCAATTAGTAAAGTATAATGAGGAAGAATATGCAAGAATTGGTGTAATGCCTCGCTACGGTGACGCAAGTTCAATCCGGTGGTTTAAAGTTGAACCCAATTGCACATTTCACATCATCAATAGTTTTGAAGCTGGTGATGAGGTTGTAGTGTGGGGTTGTAAAGCTCTTGACTCAGTTATAGGTCCAAGTAATGAAAGCAGTACTGATAATAGCACCTCAACGGCAGAGAATGCTAGTAAATTATACTCCCATGCTTATGAATGGAGATTAAACATGGAGACTGGAGAAGTCAGGGAGAGATATCTCACTGGCAAAAAGTTTTCCATGGATTTTCCTATGATCAATGGAAACTTTTGCGGCCTTAAAAACAGATTTGGGTATGCCCAAGTCGTCGATTCAGTTGCGAGCTCTACCTCAGGGTTGCTTAAGTATGGTGGTATAGCTAAGCTACATTTTGAAGAGCCAGCTGAGAGAGAAGGCGAATTAGAAAATGAGGAAATAAAGGTAGAAAGACATATGTTTGAGGAGAACACCTTTTGTACCGGAGCTGCTTTTGTCCCTAAACAAAGTGATAGTAGCAGGACTGGTAGTATTATTCTTGAAGAAGTAGAAGAAGATGATGGATGGGTTATTACTTTTGTTCACAATGAAGATACCAAAGTATCACAAGTTTATGTTATTGATACCAAGGAGTTTTGCAGTGAGCCTGTAGCCAAAATTACATTGCCCTGCAGAGTTCCATATGGATTTCATGGAGCTTTTATGCCAACCTCATTGAACACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RPE65

Protein Analysis

605

Amino Acids

67.66

Weight (kDa)

5.63

Isoelectric Point (pI)

39.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPE65 PF03055 92 - 600 7.8e-111 Retinal pigment epithelial membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 474
AccB7I CCANNNNNTGG 1 cut(s) 916
AccI GTMKAC 1 cut(s) 342
AccIII TCCGGA 1 cut(s) 331
AciI CCGC 2 cut(s) 634, 1389
AclWI GGATC 1 cut(s) 927
AcsI RAATTY 2 cut(s) 218, 315
AcyI GRCGYC 2 cut(s) 195, 471
AdeI CACNNNGTG 1 cut(s) 916
AfaI GTAC 5 cut(s) 74, 509, 656, 1234, 1573
AfiI CCNNNNNNNGG 6 cut(s) 371, 584, 745, 824, 916, 1088
AflII CTTAAG 2 cut(s) 658, 1457
AflIII ACRYGT 1 cut(s) 520
AjnI CCWGG 1 cut(s) 817
AleI CACNNNNGTG 1 cut(s) 671
Alw21I GWGCWC 2 cut(s) 817, 1442
Alw26I GTCTC 4 cut(s) 160, 775, 1009, 1304
AlwI GGATC 1 cut(s) 927
Aor13HI TCCGGA 1 cut(s) 331
AoxI GGCC 2 cut(s) 1030, 1390
ApeKI GCWGC 2 cut(s) 939, 1580
ApoI RAATTY 2 cut(s) 218, 315
AseI ATTAAT 1 cut(s) 905
Asp700I GAANNNNTTC 2 cut(s) 164, 254
AspS9I GGNCC 3 cut(s) 356, 502, 1214
AsuHPI GGTGA 3 cut(s) 281, 1103, 1178
AvaII GGWCC 3 cut(s) 356, 502, 1214
AxyI CCTNAGG 1 cut(s) 1447
BaeI ACNNNNGTAYC 4 cut(s) 1677, 1677, 1710, 1710
BanII GRGCYC 2 cut(s) 817, 1442
BauI CACGAG 1 cut(s) 298
Bbv12I GWGCWC 2 cut(s) 817, 1442
BbvCI CCTCAGC 1 cut(s) 49
BbvI GCAGC 2 cut(s) 951, 1567
BccI CCATC 6 cut(s) 250, 479, 489, 887, 1646, 1650
BceAI ACGGC 1 cut(s) 1269
BcgI CGANNNNNNTGC 2 cut(s) 1415, 1449
BciT130I CCWGG 1 cut(s) 819
BclI TGATCA 3 cut(s) 226, 981, 1371
BcoDI GTCTC 4 cut(s) 160, 775, 1009, 1304
BfaI CTAG 2 cut(s) 59, 1266
BfmI CTRYAG 3 cut(s) 1002, 1740, 1762
BfrI CTTAAG 2 cut(s) 658, 1457
BisI GCNGC 3 cut(s) 940, 1390, 1581
BlpI GCTNAGC 1 cut(s) 1476
BlsI GCNGC 3 cut(s) 941, 1391, 1582
BmcAI AGTACT 3 cut(s) 74, 656, 1234
Bme1390I CCNGG 1 cut(s) 819
Bme18I GGWCC 3 cut(s) 356, 502, 1214
BmgT120I GGNCC 3 cut(s) 356, 502, 1214
BmrFI CCNGG 1 cut(s) 819
BmrI ACTGGG 2 cut(s) 257, 851
BmsI GCATC 2 cut(s) 117, 312
BmuI ACTGGG 2 cut(s) 257, 851
BpmI CTGGAG 3 cut(s) 795, 801, 1335
Bpu10I CCTNAGC 1 cut(s) 49
Bpu1102I GCTNAGC 1 cut(s) 1476
BsaAI YACGTR 1 cut(s) 346
BsaBI GATNNNNATC 2 cut(s) 382, 734
BsaHI GRCGYC 2 cut(s) 195, 471
BsaJI CCNNGG 3 cut(s) 93, 1356, 1719
BsaWI WCCGGW 4 cut(s) 331, 504, 1107, 1574
BsaXI ACNNNNNCTCC 6 cut(s) 273, 303, 330, 360, 819, 849
Bsc4I CCNNNNNNNGG 6 cut(s) 371, 584, 745, 824, 916, 1088
Bse118I RCCGGY 1 cut(s) 598
Bse1I ACTGG 5 cut(s) 263, 846, 1318, 1345, 1621
Bse21I CCTNAGG 1 cut(s) 1447
Bse3DI GCAATG 2 cut(s) 233, 1754
Bse8I GATNNNNATC 2 cut(s) 382, 734
BseAI TCCGGA 1 cut(s) 331
BseBI CCWGG 1 cut(s) 819
BseDI CCNNGG 3 cut(s) 93, 1356, 1719
BseGI GGATG 3 cut(s) 132, 772, 1661
BseJI GATNNNNATC 2 cut(s) 382, 734
BseLI CCNNNNNNNGG 6 cut(s) 371, 584, 745, 824, 916, 1088
BseMI GCAATG 2 cut(s) 233, 1754
BseMII CTCAG 6 cut(s) 63, 260, 714, 1218, 1461, 1491
BseNI ACTGG 5 cut(s) 263, 846, 1318, 1345, 1621
BseRI GAGGAG 3 cut(s) 82, 611, 1571
BseXI GCAGC 2 cut(s) 951, 1567
BsgI GTGCAG 1 cut(s) 958
BshFI GGCC 2 cut(s) 1032, 1392
BsiHKAI GWGCWC 2 cut(s) 817, 1442
BsiSI CCGG 5 cut(s) 332, 505, 599, 1108, 1575
BslFI GGGAC 1 cut(s) 1574
BslI CCNNNNNNNGG 6 cut(s) 371, 584, 745, 824, 916, 1088
BsmAI GTCTC 4 cut(s) 160, 775, 1009, 1304
BsmFI GGGAC 1 cut(s) 1574
BsmI GAATGC 2 cut(s) 682, 1267
BsnI GGCC 2 cut(s) 1032, 1392
Bsp1286I GDGCHC 2 cut(s) 817, 1442
Bsp13I TCCGGA 1 cut(s) 331
Bsp143I GATC 4 cut(s) 226, 919, 981, 1371
Bsp1720I GCTNAGC 1 cut(s) 1476
Bsp19I CCATGG 1 cut(s) 1356
BspACI CCGC 2 cut(s) 634, 1389
BspANI GGCC 2 cut(s) 1032, 1392
BspCNI CTCAG 6 cut(s) 62, 259, 713, 1217, 1460, 1492
BspEI TCCGGA 1 cut(s) 331
BspHI TCATGA 1 cut(s) 102
BspMAI CTGCAG 1 cut(s) 1766
BspPI GGATC 1 cut(s) 927
BspQI GCTCTTC 1 cut(s) 1485
BspTI CTTAAG 2 cut(s) 658, 1457
BsrDI GCAATG 2 cut(s) 233, 1754
BsrFI RCCGGY 1 cut(s) 598
BsrI ACTGG 5 cut(s) 263, 846, 1318, 1345, 1621
BssAI RCCGGY 1 cut(s) 598
BssECI CCNNGG 3 cut(s) 93, 1356, 1719
BssMI GATC 4 cut(s) 226, 919, 981, 1371
BssNI GRCGYC 2 cut(s) 195, 471
BssSI CACGAG 1 cut(s) 298
BssT1I CCWWGG 2 cut(s) 1356, 1719
Bst2BI CACGAG 1 cut(s) 298
Bst2UI CCWGG 1 cut(s) 819
Bst4CI ACNGT 3 cut(s) 502, 864, 1091
Bst6I CTCTTC 2 cut(s) 803, 1485
BstACI GRCGYC 2 cut(s) 195, 471
BstAFI CTTAAG 2 cut(s) 658, 1457
BstAPI GCANNNNNTGC 2 cut(s) 945, 1262
BstBAI YACGTR 1 cut(s) 346
BstC8I GCNNGC 3 cut(s) 600, 1438, 1497
BstDEI CTNAG 7 cut(s) 49, 246, 700, 1204, 1447, 1476, 1500
BstDSI CCRYGG 1 cut(s) 1356
BstF5I GGATG 3 cut(s) 132, 772, 1661
BstKTI GATC 4 cut(s) 229, 922, 984, 1374
BstMAI GTCTC 4 cut(s) 160, 775, 1009, 1304
BstMBI GATC 4 cut(s) 226, 919, 981, 1371
BstMWI GCNNNNNNNGC 2 cut(s) 945, 1262
BstNI CCWGG 1 cut(s) 819
BstNSI RCATGY 1 cut(s) 524
BstSCI CCNGG 1 cut(s) 817
BstSFI CTRYAG 3 cut(s) 1002, 1740, 1762
BstSNI TACGTA 1 cut(s) 346
BstV1I GCAGC 2 cut(s) 951, 1567
BstX2I RGATCY 1 cut(s) 919
BstYI RGATCY 1 cut(s) 919
Bsu36I CCTNAGG 1 cut(s) 1447
BsuRI GGCC 2 cut(s) 1032, 1392
BtgI CCRYGG 1 cut(s) 1356
BtsCI GGATG 3 cut(s) 132, 772, 1661
BtsI GCAGTG 2 cut(s) 487, 1738
BtsIMutI CAGTG 3 cut(s) 487, 1338, 1738
Cac8I GCNNGC 3 cut(s) 600, 1438, 1497
CciI TCATGA 1 cut(s) 102
Cfr10I RCCGGY 1 cut(s) 598
Cfr13I GGNCC 3 cut(s) 356, 502, 1214
CpoI CGGWCCG 1 cut(s) 502
CseI GACGC 3 cut(s) 203, 860, 1103
Csp6I GTAC 5 cut(s) 73, 508, 655, 1233, 1572
CspI CGGWCCG 1 cut(s) 502
CviQI GTAC 5 cut(s) 73, 508, 655, 1233, 1572
DdeI CTNAG 7 cut(s) 49, 246, 700, 1204, 1447, 1476, 1500
DpnI GATC 4 cut(s) 228, 921, 983, 1373
DpnII GATC 4 cut(s) 226, 919, 981, 1371
DraI TTTAAA 1 cut(s) 1117
DraIII CACNNNGTG 1 cut(s) 916
Eam1104I CTCTTC 2 cut(s) 803, 1485
EarI CTCTTC 2 cut(s) 803, 1485
Ecl136II GAGCTC 2 cut(s) 815, 1440
Eco105I TACGTA 1 cut(s) 346
Eco130I CCWWGG 2 cut(s) 1356, 1719
Eco24I GRGCYC 2 cut(s) 817, 1442
Eco32I GATATC 2 cut(s) 736, 1334
Eco47I GGWCC 3 cut(s) 356, 502, 1214
Eco53kI GAGCTC 2 cut(s) 815, 1440
Eco81I CCTNAGG 1 cut(s) 1447
EcoICRI GAGCTC 2 cut(s) 815, 1440
EcoRI GAATTC 2 cut(s) 218, 315
EcoRII CCWGG 1 cut(s) 817
EcoRV GATATC 2 cut(s) 736, 1334
EcoT14I CCWWGG 2 cut(s) 1356, 1719
EcoT22I ATGCAT 1 cut(s) 147
EcoT38I GRGCYC 2 cut(s) 817, 1442
ErhI CCWWGG 2 cut(s) 1356, 1719
FaqI GGGAC 1 cut(s) 1574
FauNDI CATATG 2 cut(s) 1548, 1774
FbaI TGATCA 3 cut(s) 226, 981, 1371
FblI GTMKAC 1 cut(s) 342
Fnu4HI GCNGC 3 cut(s) 940, 1390, 1581
FokI GGATG 3 cut(s) 139, 779, 1668
FriOI GRGCYC 2 cut(s) 817, 1442
Fsp4HI GCNGC 3 cut(s) 940, 1390, 1581
FspBI CTAG 2 cut(s) 59, 1266
GluI GCNGC 3 cut(s) 940, 1390, 1581
GsuI CTGGAG 3 cut(s) 795, 801, 1335
HaeIII GGCC 2 cut(s) 1032, 1392
HapII CCGG 5 cut(s) 332, 505, 599, 1108, 1575
HgaI GACGC 3 cut(s) 203, 860, 1103
Hin1I GRCGYC 2 cut(s) 195, 471
HindIII AAGCTT 1 cut(s) 542
HinfI GANTC 7 cut(s) 106, 206, 339, 593, 1017, 1202, 1427
HpaII CCGG 5 cut(s) 332, 505, 599, 1108, 1575
HphI GGTGA 3 cut(s) 281, 1103, 1178
Hpy166II GTNNAC 4 cut(s) 343, 649, 850, 1675
Hpy188I TCNGA 3 cut(s) 36, 249, 311
Hpy8I GTNNAC 4 cut(s) 343, 649, 850, 1675
Hpy99I CGWCG 4 cut(s) 391, 473, 476, 1427
HpyAV CCTTC 8 cut(s) 117, 272, 278, 399, 422, 428, 576, 1502
HpyCH4III ACNGT 3 cut(s) 502, 864, 1091
HpyCH4IV ACGT 2 cut(s) 345, 471
HpyF10VI GCNNNNNNNGC 2 cut(s) 945, 1262
HpyF3I CTNAG 7 cut(s) 49, 246, 700, 1204, 1447, 1476, 1500
HpySE526I ACGT 2 cut(s) 345, 471
Hsp92I GRCGYC 2 cut(s) 195, 471
Kpn2I TCCGGA 1 cut(s) 331
KroI GCCGGC 1 cut(s) 598
KroNI GCCGGC 1 cut(s) 600
Ksp22I TGATCA 3 cut(s) 226, 981, 1371
Kzo9I GATC 4 cut(s) 226, 919, 981, 1371
LguI GCTCTTC 1 cut(s) 1485
LmnI GCTCC 4 cut(s) 776, 820, 1577, 1787
Lsp1109I GCAGC 2 cut(s) 951, 1567
LweI GCATC 2 cut(s) 117, 312
MaeI CTAG 2 cut(s) 59, 1266
MaeII ACGT 2 cut(s) 345, 471
MaeIII GTNAC 2 cut(s) 961, 1091
MalI GATC 4 cut(s) 228, 921, 983, 1373
MboI GATC 4 cut(s) 226, 919, 981, 1371
MfeI CAATTG 2 cut(s) 760, 1129
MflI RGATCY 1 cut(s) 919
MhlI GDGCHC 2 cut(s) 817, 1442
MlyI GAGTC 4 cut(s) 348, 587, 1011, 1196
MmeI TCCRAC 1 cut(s) 171
Mph1103I ATGCAT 1 cut(s) 147
MroI TCCGGA 1 cut(s) 331
MroNI GCCGGC 1 cut(s) 598
MroXI GAANNNNTTC 2 cut(s) 164, 254
MseI TTAA 7 cut(s) 659, 858, 905, 1116, 1301, 1395, 1458
MslI CAYNNNNRTG 3 cut(s) 150, 671, 1287
MspA1I CMGCKG 2 cut(s) 53, 1499
MspCI CTTAAG 2 cut(s) 658, 1457
MspI CCGG 5 cut(s) 332, 505, 599, 1108, 1575
MspR9I CCNGG 1 cut(s) 819
MunI CAATTG 2 cut(s) 760, 1129
Mva1269I GAATGC 2 cut(s) 682, 1267
MvaI CCWGG 1 cut(s) 819
MwoI GCNNNNNNNGC 2 cut(s) 945, 1262
NaeI GCCGGC 1 cut(s) 600
NcoI CCATGG 1 cut(s) 1356
NdeI CATATG 2 cut(s) 1548, 1774
NdeII GATC 4 cut(s) 226, 919, 981, 1371
NgoMIV GCCGGC 1 cut(s) 598
NmeAIII GCCGAG 1 cut(s) 74
NmuCI GTSAC 1 cut(s) 1091
NsiI ATGCAT 1 cut(s) 147
NspI RCATGY 1 cut(s) 524
OliI CACNNNNGTG 1 cut(s) 671
PagI TCATGA 1 cut(s) 102
PciI ACATGT 1 cut(s) 520
PciSI GCTCTTC 1 cut(s) 1485
PctI GAATGC 2 cut(s) 682, 1267
PdiI GCCGGC 1 cut(s) 600
PdmI GAANNNNTTC 2 cut(s) 164, 254
PfeI GAWTC 3 cut(s) 106, 206, 1427
PflFI GACNNNGTC 1 cut(s) 473
PflMI CCANNNNNTGG 1 cut(s) 916
PkrI GCNGC 3 cut(s) 941, 1391, 1582
PleI GAGTC 4 cut(s) 347, 587, 1011, 1196
PpsI GAGTC 4 cut(s) 347, 587, 1011, 1196
Ppu21I YACGTR 1 cut(s) 346
PscI ACATGT 1 cut(s) 520
PshBI ATTAAT 1 cut(s) 905
Psp124BI GAGCTC 2 cut(s) 817, 1442
Psp6I CCWGG 1 cut(s) 817
PspGI CCWGG 1 cut(s) 817
PspPI GGNCC 3 cut(s) 356, 502, 1214
PstI CTGCAG 1 cut(s) 1766
PsuI RGATCY 1 cut(s) 919
PsyI GACNNNGTC 1 cut(s) 473
PvuII CAGCTG 2 cut(s) 53, 1499
RsaI GTAC 5 cut(s) 74, 509, 656, 1234, 1573
RsaNI GTAC 5 cut(s) 73, 508, 655, 1233, 1572
RseI CAYNNNNRTG 3 cut(s) 150, 671, 1287
Rsr2I CGGWCCG 1 cut(s) 502
RsrII CGGWCCG 1 cut(s) 502
SacI GAGCTC 2 cut(s) 817, 1442
SapI GCTCTTC 1 cut(s) 1485
SaqAI TTAA 7 cut(s) 659, 858, 905, 1116, 1301, 1395, 1458
SatI GCNGC 3 cut(s) 940, 1390, 1581
Sau3AI GATC 4 cut(s) 226, 919, 981, 1371
Sau96I GGNCC 3 cut(s) 356, 502, 1214
ScaI AGTACT 3 cut(s) 74, 656, 1234
SchI GAGTC 4 cut(s) 348, 587, 1011, 1196
ScrFI CCNGG 1 cut(s) 819
SduI GDGCHC 2 cut(s) 817, 1442
SfaNI GCATC 2 cut(s) 117, 312
SfcI CTRYAG 3 cut(s) 1002, 1740, 1762
SinI GGWCC 3 cut(s) 356, 502, 1214
SmiMI CAYNNNNRTG 3 cut(s) 150, 671, 1287
SmlI CTYRAG 2 cut(s) 658, 1457
SmoI CTYRAG 2 cut(s) 658, 1457
SnaBI TACGTA 1 cut(s) 346
SsiI CCGC 2 cut(s) 634, 1389
SspMI CTAG 2 cut(s) 59, 1266
SstI GAGCTC 2 cut(s) 817, 1442
StyD4I CCNGG 1 cut(s) 817
StyI CCWWGG 2 cut(s) 1356, 1719
TaaI ACNGT 3 cut(s) 502, 864, 1091
TaiI ACGT 2 cut(s) 348, 474
TaqI TCGA 4 cut(s) 158, 386, 525, 1425
TatI WGTACW 3 cut(s) 72, 654, 1232
TauI GCSGC 1 cut(s) 1392
TfiI GAWTC 3 cut(s) 106, 206, 1427
Tru1I TTAA 7 cut(s) 659, 858, 905, 1116, 1301, 1395, 1458
Tru9I TTAA 7 cut(s) 659, 858, 905, 1116, 1301, 1395, 1458
TscAI CASTG 3 cut(s) 487, 1345, 1738
TseFI GTSAC 1 cut(s) 1091
TseI GCWGC 2 cut(s) 939, 1580
Tsp45I GTSAC 1 cut(s) 1091
TspDTI ATGAA 8 cut(s) 119, 211, 434, 966, 1239, 1305, 1695, 1772
TspRI CASTG 3 cut(s) 487, 1345, 1738
Tth111I GACNNNGTC 1 cut(s) 473
Van91I CCANNNNNTGG 1 cut(s) 916
Vha464I CTTAAG 2 cut(s) 658, 1457
VpaK11BI GGWCC 3 cut(s) 356, 502, 1214
VspI ATTAAT 1 cut(s) 905
XapI RAATTY 2 cut(s) 218, 315
XceI RCATGY 1 cut(s) 524
XmiI GTMKAC 1 cut(s) 342
XmnI GAANNNNTTC 2 cut(s) 164, 254
XspI CTAG 2 cut(s) 59, 1266
ZraI GACGTC 1 cut(s) 472
ZrmI AGTACT 3 cut(s) 74, 656, 1234
Zsp2I ATGCAT 1 cut(s) 147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.