MD15G1094100.v1.1

Carotenoid 9,10(9',10')-cleavage dioxygenase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0013626
Physical Location & Seq
Forward (+)
6603143 .. 6604571
1429 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1094100.v1.1.491

Sequence Viewer

Length: 738 bp
ATGTGTGCAATGTTATATATATTGCTTGATCTGCTGGTTTTGATATGCAGATTAGTAGAGTACAATCATGAAGGATGTGCAAGAATAGGTGTGATGCCACGCTACGGAGATGCAGATTCAGTCCGCTGGTTTAAAGTTGAACCCAATTGCACATTTCACATCATCAATAGTTTTGAAGATGGTGATGAGGTTGTTGTGTGGGCTTGTAAAGCTCTGGACTCAGTTATACCAGGACCTGACATGGCATTGAATCAATTTGGGTGGTTGCCAAGAAAATTGAAGCCTACAAGTACAAGTAAAGAAAATGATATCTCACCAGATAAGGGAAACTTACTTTCTCATGCTTATGAATGGAGATTAAACATGCAAAGTGGGGAAGTCAATGAGAAATATCTCACTGGAAAAAGGAATGCTCAAATACAGTGGTCTAGCACTTTGAAGAACCAGCTGGTGTTTCTTCGCGAAGGCCAATTAGAAGAGGAAATAAAGGTGGAAAATCATATGTTTGAGGAGAACAGCTTTTGCAGTGGAGCTTCTTTTGTCCCAAACCGAAGGGGTCTTGAAGAAGATGATGGATGGGTTATCACTTTTGTTCACAACGAAGATACCAACATATCTCAAGTTTATGTGATTGACACAAAGAAAATTTCTAGTGAGCCTGTTGCTAAAATTACATTGCCACGTAGAGTTCCATACGGATTTCATGGAGCTTTCAAGCCAATCTCATCCTCCACGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RPE65

Protein Analysis

246

Amino Acids

27.91

Weight (kDa)

5.44

Isoelectric Point (pI)

39.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPE65 PF03055 19 - 240 2.9e-38 Retinal pigment epithelial membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 462
AciI CCGC 1 cut(s) 124
AcsI RAATTY 1 cut(s) 645
AfaI GTAC 2 cut(s) 62, 292
AfiI CCNNNNNNNGG 2 cut(s) 104, 323
AgsI TTSAA 7 cut(s) 140, 176, 250, 280, 439, 563, 715
AjnI CCWGG 1 cut(s) 229
AluBI AGCT 5 cut(s) 212, 448, 519, 533, 710
AluI AGCT 5 cut(s) 212, 448, 519, 533, 710
AlwNI CAGNNNCTG 1 cut(s) 236
AoxI GGCC 1 cut(s) 466
ApoI RAATTY 1 cut(s) 645
AspS9I GGNCC 1 cut(s) 233
AsuHPI GGTGA 2 cut(s) 194, 306
AvaII GGWCC 1 cut(s) 233
BccI CCATC 3 cut(s) 173, 566, 570
BciT130I CCWGG 1 cut(s) 231
BfaI CTAG 2 cut(s) 429, 651
Bme1390I CCNGG 1 cut(s) 231
Bme18I GGWCC 1 cut(s) 233
BmgT120I GGNCC 1 cut(s) 233
BmrFI CCNGG 1 cut(s) 231
BmsI GCATC 2 cut(s) 84, 100
BpuEI CTTGAG 1 cut(s) 603
BsaAI YACGTR 2 cut(s) 683, 735
Bsc4I CCNNNNNNNGG 2 cut(s) 104, 323
Bse1I ACTGG 1 cut(s) 403
Bse3DI GCAATG 2 cut(s) 15, 674
BseBI CCWGG 1 cut(s) 231
BseGI GGATG 3 cut(s) 80, 581, 725
BseLI CCNNNNNNNGG 2 cut(s) 104, 323
BseMI GCAATG 2 cut(s) 15, 674
BseMII CTCAG 1 cut(s) 234
BseNI ACTGG 1 cut(s) 403
BseRI GAGGAG 1 cut(s) 524
Bsh1236I CGCG 1 cut(s) 462
BshFI GGCC 1 cut(s) 468
BslFI GGGAC 1 cut(s) 527
BslI CCNNNNNNNGG 2 cut(s) 104, 323
BsmFI GGGAC 1 cut(s) 527
BsmI GAATGC 1 cut(s) 415
BsnI GGCC 1 cut(s) 468
Bsp143I GATC 1 cut(s) 28
Bsp68I TCGCGA 1 cut(s) 462
BspACI CCGC 1 cut(s) 124
BspANI GGCC 1 cut(s) 468
BspCNI CTCAG 1 cut(s) 233
BspFNI CGCG 1 cut(s) 462
BspHI TCATGA 1 cut(s) 67
BsrDI GCAATG 2 cut(s) 15, 674
BsrI ACTGG 1 cut(s) 403
BssMI GATC 1 cut(s) 28
Bst2UI CCWGG 1 cut(s) 231
Bst4CI ACNGT 1 cut(s) 423
Bst6I CTCTTC 1 cut(s) 471
BstBAI YACGTR 2 cut(s) 683, 735
BstDEI CTNAG 1 cut(s) 220
BstF5I GGATG 3 cut(s) 80, 581, 725
BstFNI CGCG 1 cut(s) 462
BstKTI GATC 1 cut(s) 31
BstMBI GATC 1 cut(s) 28
BstMWI GCNNNNNNNGC 2 cut(s) 31, 209
BstNI CCWGG 1 cut(s) 231
BstNSI RCATGY 1 cut(s) 367
BstSCI CCNGG 1 cut(s) 229
BstUI CGCG 1 cut(s) 462
BsuRI GGCC 1 cut(s) 468
BtsCI GGATG 3 cut(s) 80, 581, 725
BtsI GCAGTG 1 cut(s) 532
BtsIMutI CAGTG 3 cut(s) 396, 428, 532
BtuMI TCGCGA 1 cut(s) 462
CaiI CAGNNNCTG 1 cut(s) 236
CciI TCATGA 1 cut(s) 67
Cfr13I GGNCC 1 cut(s) 233
Csp6I GTAC 2 cut(s) 61, 291
CspCI CAANNNNNGTGG 4 cut(s) 242, 277, 404, 439
CviAII CATG 5 cut(s) 68, 241, 341, 364, 704
CviQI GTAC 2 cut(s) 61, 291
DdeI CTNAG 1 cut(s) 220
DpnI GATC 1 cut(s) 30
DpnII GATC 1 cut(s) 28
DraI TTTAAA 1 cut(s) 133
Eam1104I CTCTTC 1 cut(s) 471
EarI CTCTTC 1 cut(s) 471
Eco32I GATATC 1 cut(s) 310
Eco47I GGWCC 1 cut(s) 233
EcoO109I RGGNCCY 1 cut(s) 233
EcoRII CCWGG 1 cut(s) 229
EcoRV GATATC 1 cut(s) 310
FaeI CATG 5 cut(s) 71, 244, 344, 367, 707
FalI AAGNNNNNCTT 2 cut(s) 314, 346
FaqI GGGAC 1 cut(s) 527
FatI CATG 5 cut(s) 67, 240, 340, 363, 703
FauNDI CATATG 1 cut(s) 501
FokI GGATG 3 cut(s) 87, 588, 712
FspBI CTAG 2 cut(s) 429, 651
HaeIII GGCC 1 cut(s) 468
Hin1II CATG 5 cut(s) 71, 244, 344, 367, 707
HinfI GANTC 3 cut(s) 116, 218, 250
HphI GGTGA 2 cut(s) 194, 306
Hpy166II GTNNAC 1 cut(s) 595
Hpy188III TCNNGA 4 cut(s) 68, 215, 461, 560
Hpy8I GTNNAC 1 cut(s) 595
HpyAV CCTTC 3 cut(s) 65, 458, 546
HpyCH4III ACNGT 1 cut(s) 423
HpyCH4IV ACGT 2 cut(s) 682, 734
HpyCH4V TGCA 7 cut(s) 8, 48, 80, 113, 150, 367, 525
HpyF10VI GCNNNNNNNGC 2 cut(s) 31, 209
HpyF3I CTNAG 1 cut(s) 220
HpySE526I ACGT 2 cut(s) 682, 734
Hsp92II CATG 5 cut(s) 71, 244, 344, 367, 707
Kzo9I GATC 1 cut(s) 28
LmnI GCTCC 2 cut(s) 530, 707
LweI GCATC 2 cut(s) 84, 100
MaeI CTAG 2 cut(s) 429, 651
MaeII ACGT 2 cut(s) 682, 734
MalI GATC 1 cut(s) 30
MboI GATC 1 cut(s) 28
MboII GAAGA 7 cut(s) 188, 449, 451, 488, 575, 578, 614
MfeI CAATTG 1 cut(s) 145
MluCI AATT 6 cut(s) 145, 254, 275, 470, 645, 669
MlyI GAGTC 1 cut(s) 212
MnlI CCTC 3 cut(s) 181, 472, 502
MseI TTAA 2 cut(s) 132, 359
MslI CAYNNNNRTG 1 cut(s) 345
MspA1I CMGCKG 2 cut(s) 126, 448
MspR9I CCNGG 1 cut(s) 231
MunI CAATTG 1 cut(s) 145
Mva1269I GAATGC 1 cut(s) 415
MvaI CCWGG 1 cut(s) 231
MvnI CGCG 1 cut(s) 462
MwoI GCNNNNNNNGC 2 cut(s) 31, 209
NdeI CATATG 1 cut(s) 501
NdeII GATC 1 cut(s) 28
NlaIII CATG 5 cut(s) 71, 244, 344, 367, 707
NruI TCGCGA 1 cut(s) 462
NspI RCATGY 1 cut(s) 367
PagI TCATGA 1 cut(s) 67
PctI GAATGC 1 cut(s) 415
PfeI GAWTC 2 cut(s) 116, 250
PleI GAGTC 1 cut(s) 212
PpsI GAGTC 1 cut(s) 212
Ppu21I YACGTR 2 cut(s) 683, 735
PpuMI RGGWCCY 1 cut(s) 233
Psp5II RGGWCCY 1 cut(s) 233
Psp6I CCWGG 1 cut(s) 229
PspGI CCWGG 1 cut(s) 229
PspPI GGNCC 1 cut(s) 233
PspPPI RGGWCCY 1 cut(s) 233
PstNI CAGNNNCTG 1 cut(s) 236
PvuII CAGCTG 1 cut(s) 448
RruI TCGCGA 1 cut(s) 462
RsaI GTAC 2 cut(s) 62, 292
RsaNI GTAC 2 cut(s) 61, 291
RseI CAYNNNNRTG 1 cut(s) 345
SaqAI TTAA 2 cut(s) 132, 359
Sau3AI GATC 1 cut(s) 28
Sau96I GGNCC 1 cut(s) 233
SchI GAGTC 1 cut(s) 212
ScrFI CCNGG 1 cut(s) 231
SfaNI GCATC 2 cut(s) 84, 100
SinI GGWCC 1 cut(s) 233
SmiMI CAYNNNNRTG 1 cut(s) 345
SmlI CTYRAG 1 cut(s) 618
SmoI CTYRAG 1 cut(s) 618
Sse9I AATT 6 cut(s) 145, 254, 275, 470, 645, 669
SsiI CCGC 1 cut(s) 124
SspMI CTAG 2 cut(s) 429, 651
StyD4I CCNGG 1 cut(s) 229
TaaI ACNGT 1 cut(s) 423
TaiI ACGT 2 cut(s) 685, 737
TasI AATT 6 cut(s) 145, 254, 275, 470, 645, 669
TatI WGTACW 2 cut(s) 60, 290
TfiI GAWTC 2 cut(s) 116, 250
Tru1I TTAA 2 cut(s) 132, 359
Tru9I TTAA 2 cut(s) 132, 359
TscAI CASTG 3 cut(s) 403, 428, 532
TspDTI ATGAA 3 cut(s) 84, 363, 692
TspGWI ACGGA 2 cut(s) 120, 711
TspRI CASTG 3 cut(s) 403, 428, 532
VpaK11BI GGWCC 1 cut(s) 233
XapI RAATTY 1 cut(s) 645
XceI RCATGY 1 cut(s) 367
XspI CTAG 2 cut(s) 429, 651
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.