MD08G1117600.v1.1
MYB Family

Myb-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0006637
Physical Location & Seq
Reverse (-)
10744563 .. 10750683
6121 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1117600.v1.1.491

Sequence Viewer

Length: 3063 bp
ATGGAAGGTGAAAGAACAAATTCTACTCCTTCAGAAGTGCTTGGTGACAACAATCAGAAAGCTCGAGCTCTCCATGGGAGGACTAGTGGGCCTACGAGGCGTTCTACGAAGGGACAATGGACACCTGAAGAGGATGAGATCTTGCGGAGGGCTGTTCAGCGTTTTAAAGGAAAGAATTGGAAAAAAATAGCGGAGTGTTTCAAGGATCGGACTGATGTGCAATGCCTACATAGATGGCAGAAAGTCTTGAATCCCGAACTGGTCAAAGGTCCGTGGTCTAAAGAGGAGGATGATGTTATTATTGAGTTGGTGAAAAAATATGGTCCAAAAAAGTGGTCCACTATTGCACAGCATTTACCTGGACGTATTGGTAAGCAGTGTCGGGAAAGGTGGCATAATCATCTTAATCCTGGCATAAACAAAGAGGCATGGACACAGGAAGAGGAGTTGGCTCTGATTCGTGCTCATCAAATGTATGGGAACAAATGGGCAGAGCTAACGAAGTTCTTGCCTGGAAGGACAGACAATGCTATAAAAAATCACTGGAACAGTTCTGTGAAGAAGAAGTTGGATTCTTACTTGAAATCAGGTTTACTTACACAGTTTCAAGGAATGCCTCATGTTGGAAATCAAAGCCAACACATGATGTCATCTTCTTCAAGGATGCAGAGCAGTGGAGATGATAGTGGTGCCAAAGGTGCAGAAGCCGAAGAAATTTCAGAATGCAGTCAAGATTCAACCGTTGCTGGTCGCTCTGCACAAATGGCCAATGTGGTACCTCATCCTATAGAGGAATTTCAGAATAATGAAGTATCCCATTTAGCAAATGATCCAAGCTTCAGTCCAGTATCTTGTTCAGAACCATATTACCCATCTATAGGAGATGCCAATGTTTCCATCCCAGAAATACCTCCTGAATTGGTTTGCTCCAAATACCTGGAACAAAATTTCTCACATGATGCTGGAGCTTCGATGAGTGGGGATTTCCAGTTAAATTTGCATGAGTTACCTAACATTTCTTCACTAGAATGCGGCCAGGAATCATTAAGGATGCATGGAGGGGTGGAGGCTCCGTTTCAAACTTCTACTTCCATGGGGAACATGGCCGTGGGTTCAGTTAAATCAGAGCACATGTTGATATCTGACGATGAATGCTGCAGGGTATTATTTTCAATGAATGATGGGTGTTTTCCCTCTAGAGATTTTACAGATGATTCAAATATGGTTGATTTGGGTGCTTGCACAGATTCAGTACTTCTACAACCATCAAACCTCCAAATATCTGACCCTGGCAGAACTTCAGCTTCACAATTATATCATCCTCTGAATTCTGGTGTAATTGGAACTTCTTGCTCTCAAGTAGTTTCTGCTCATGAAACTCCACTGATATATGCTGGAGAACCTACTCATTTATTCATAGTTCAAGATCAAGAGTTTGTGACAAGTTCAAATGATGGATTTATCTATACTAACGAGTCTGCCAGTTCTCCCTGCAAATCCGATCTGGTTAGTGATTCTTCAAAACTAGTCCCTGTAAATACATTTGCTTTGGGATTGGACGCGCAAACTTGTCCTGTGGATGTGAGTTCAAATGAGCAGACAGAACAGCAGGATGCTGGCTCTCTATGTTATGAACCTCCTCGCTTTCCAAGCTTGGATATTCCATTTTTTAGTTGTGATCTTATACAATCTGGTAATGACATGCAGCAAGAATATAGCCCCCTCGGCATTCGTCAGCTGATGATGTCTTCTATGAATTGCCTCACTCCATATAGGTTATGGGATTCACCCTCGCGTGAGGGAAGCCCTGATGCCTTGCTAAAAAGTGCTGCTAAAACTTTTACAGGCACGCCATCTATTTTGAAGAAACGACACCGAGATTTGTTGTCTCCATTGTCGCCATTGTCCGATAGAAGATTTGATAAAAGGGTTGGGACTGACGTGACCTCCTGCTTGGCCAGAGATTTTTCACGTCTAGATGTTATGTTTGAAGATACAGAGAACAAAGAAGATTCACTTTCTCCATCATCTGATCAGAAAAGGAATTCTGATGCCTCTGTTGAAGATAAAGAGAACAAGGGTACCTGTGAAAACAAAGTAGAAAAAGGGATTGACAGCACCGCAATGTCAGATGATGGAACTGCACAGAAAGATGTTGATGACGGTCAATCCCAGGAAAAGAGAAAGCAGTTCCAACAAACTTCTGATGTTGATGCTGAGAGTAAGGTTGATCTCGTCCCAACTACACAAAGTGCACAACCTTCTGGAGTTCTTGTTGAACGTAACACAAATGATCTGCTACTGAATTCTCCTGATGTAGTTGGTTGTAAAGCTGAAAAACCCTTCGGCTCAACTGCTAGAACTCCTAAAAGTCTCTTACGTAAAAGCTTTGAAGCCACCAACCCAGGAGTTCCTTCTAAATCTTTCTCTGCAAGACAATGCTCATCTGTTAAAACCCCCACCATATGCATAAAGAAGCATGAAAGTCTTGCAGATAAATGCTTACAATCTGGTTCTTCGTCAGTTCCCCCAGAGACATCTGGTGATAATGCCGGCAATGATGTCAGTATTGAAAGCATGTTCGGTGGTACTCCTTTTAAGAGAAGTATCGAATCTCCTTCCGCATGGAAATCTCCTTGGTTCATCAACTCCTTCGTCCCCGGCCCTAGTGTTGATACTGAAATTACAATTGAGGATATAGGGTTTTTCATGAGCCCAGGGGATAGAAGCTATGATGCCATTGGCCTGATGAAACAGATAAGTGAGCAGAGTGCTGCTGCCTATGCCAATGCCCAGGAGGTTTTGGGAAATGAAACTCCTGAAAGTTTATTCCAGGAAAGACAGAAAGACCGAGATATTGCGGGCCTCGAGAACAAGCAGGGGCTACCTAATCAGCCGGGGAGTGCTTCTCTTTCGGGTTCCGATGTTTTGGTGGAGCGTCGCACCCTCGACTTCAGCGAATGCGGGACGCCGGGGAAGAAGGGAACAGAAAATGTAAAGTCTGCGTCTAATGCCAAAAGCTTCTCAAGTCCGACTTCTTATTTGTTGAAGGGTTGCAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000981 GO:0000988 GO:0000989 GO:0001067 GO:0001076 GO:0001101 GO:0001134 GO:0001135 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0003712 GO:0003713 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006275 GO:0006355 GO:0006357 GO:0008150 GO:0008285 GO:0009605 GO:0009607 GO:0009608 GO:0009610 GO:0009620 GO:0009623 GO:0009719 GO:0009725 GO:0009751 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010564 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0014070 GO:0019219 GO:0019222 GO:0030154 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0032465 GO:0032502 GO:0032875 GO:0042127 GO:0042221 GO:0042493 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0046677 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048585 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051253 GO:0051254 GO:0051302 GO:0051704 GO:0051707 GO:0051726 GO:0060255 GO:0065007 GO:0080090 GO:0090329 GO:0097159 GO:0140110 GO:1901181 GO:1901363 GO:1901700 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:2000112 GO:2000113 GO:2001023 GO:2001024 GO:2001038 GO:2001039 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04151 ko04218 ko05166 map04151 map04218 map05166
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

1021

Amino Acids

111.82

Weight (kDa)

5.21

Isoelectric Point (pI)

58.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 37 - 83 3.9e-16 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 40 - 97 1.7e-17 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 89 - 135 9.9e-20 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 92 - 151 7.5e-20 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 141 - 184 5.2e-16 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 144 - 189 1.1e-08 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 775, 2081
AccB1I GGYRCC 3 cut(s) 689, 775, 2081
AccII CGCG 2 cut(s) 1562, 1795
AciI CCGC 7 cut(s) 145, 191, 1032, 2121, 2622, 2861, 2964
AclWI GGATC 2 cut(s) 213, 824
AcoI YGGCCR 4 cut(s) 765, 1033, 1104, 1956
AcsI RAATTY 8 cut(s) 19, 714, 794, 946, 994, 1327, 2044, 2305
AcuI CTGAAG 5 cut(s) 15, 147, 823, 1284, 2938
AcyI GRCGYC 1 cut(s) 2969
AdeI CACNNNGTG 1 cut(s) 2252
AfaI GTAC 4 cut(s) 777, 1254, 2083, 2590
AfiI CCNNNNNNNGG 2 cut(s) 623, 878
AflIII ACRYGT 1 cut(s) 1131
AhlI ACTAGT 2 cut(s) 83, 1525
AjiI CACGTC 2 cut(s) 1942, 1973
AjuI GAANNNNNNNTTGG 2 cut(s) 551, 583
Alw21I GWGCWC 4 cut(s) 70, 466, 1131, 2257
Alw26I GTCTC 3 cut(s) 1893, 2378, 2528
Alw44I GTGCAC 1 cut(s) 2253
AlwI GGATC 2 cut(s) 213, 824
Ama87I CYCGRG 2 cut(s) 63, 2867
AoxI GGCC 8 cut(s) 89, 765, 1033, 1104, 1956, 2662, 2743, 2863
ApaLI GTGCAC 1 cut(s) 2253
ApeKI GCWGC 5 cut(s) 1155, 1705, 1829, 2774, 2777
ApoI RAATTY 8 cut(s) 19, 714, 794, 946, 994, 1327, 2044, 2305
ArsI GACNNNNNNTTYG 4 cut(s) 320, 352, 3025, 3057
Asp700I GAANNNNTTC 1 cut(s) 19
Asp718I GGTACC 2 cut(s) 775, 2081
AspLEI GCGC 1 cut(s) 1564
AspS9I GGNCC 6 cut(s) 89, 269, 323, 336, 2663, 2863
AsuC2I CCSGG 3 cut(s) 2661, 2898, 2973
AsuHPI GGTGA 5 cut(s) 20, 56, 322, 1779, 2555
AvaI CYCGRG 2 cut(s) 63, 2867
AvaII GGWCC 3 cut(s) 269, 323, 336
BaeGI GKGCMC 1 cut(s) 2257
BaeI ACNNNNGTAYC 2 cut(s) 2065, 2098
BalI TGGCCA 2 cut(s) 767, 1958
BanI GGYRCC 3 cut(s) 689, 775, 2081
BanII GRGCYC 2 cut(s) 70, 2717
BbsI GAAGAC 1 cut(s) 1740
Bbv12I GWGCWC 4 cut(s) 70, 466, 1131, 2257
BbvI GCAGC 5 cut(s) 1142, 1717, 1816, 2761, 2764
BccI CCATC 9 cut(s) 228, 880, 905, 1175, 1273, 1448, 1861, 2032, 2129
BceAI ACGGC 1 cut(s) 1091
BcgI CGANNNNNNTGC 2 cut(s) 490, 524
BciVI GTATCC 1 cut(s) 823
BclI TGATCA 1 cut(s) 2032
BcnI CCSGG 3 cut(s) 2661, 2898, 2973
BcoDI GTCTC 3 cut(s) 1893, 2378, 2528
BcuI ACTAGT 2 cut(s) 83, 1525
BfaI CTAG 7 cut(s) 84, 1025, 1197, 1526, 1976, 2358, 2667
BfmI CTRYAG 3 cut(s) 786, 876, 1156
BfuI GTATCC 1 cut(s) 823
BglI GCCNNNNNGGC 2 cut(s) 97, 1725
BglII AGATCT 1 cut(s) 138
BisI GCNGC 6 cut(s) 1033, 1156, 1706, 1830, 2775, 2778
BlsI GCNGC 6 cut(s) 1034, 1157, 1707, 1831, 2776, 2779
BmcAI AGTACT 1 cut(s) 1254
Bme18I GGWCC 3 cut(s) 269, 323, 336
BmeT110I CYCGRG 2 cut(s) 63, 2867
BmgBI CACGTC 2 cut(s) 1942, 1973
BmgT120I GGNCC 6 cut(s) 89, 269, 323, 336, 2663, 2863
BmiI GGNNCC 5 cut(s) 691, 777, 1071, 2083, 2920
BmsI GCATC 9 cut(s) 654, 874, 949, 1041, 1603, 1801, 2041, 2203, 2725
BpiI GAAGAC 1 cut(s) 1740
BplI GAGNNNNNCTC 2 cut(s) 2893, 2925
BpmI CTGGAG 3 cut(s) 984, 1416, 2286
BpuEI CTTGAG 2 cut(s) 1341, 3010
BpuMI CCSGG 3 cut(s) 2661, 2898, 2973
BsaAI YACGTR 1 cut(s) 2381
BsaHI GRCGYC 1 cut(s) 2969
BsaXI ACNNNNNCTCC 8 cut(s) 70, 100, 1058, 1088, 1931, 1961, 2259, 2289
Bsc4I CCNNNNNNNGG 2 cut(s) 623, 878
Bse118I RCCGGY 1 cut(s) 2552
Bse1I ACTGG 5 cut(s) 264, 548, 845, 988, 1482
Bse3DI GCAATG 3 cut(s) 227, 2130, 2563
BseGI GGATG 9 cut(s) 139, 295, 669, 781, 897, 1056, 1318, 1585, 1618
BseLI CCNNNNNNNGG 2 cut(s) 623, 878
BseMI GCAATG 3 cut(s) 227, 2130, 2563
BseMII CTCAG 1 cut(s) 2208
BseNI ACTGG 5 cut(s) 264, 548, 845, 988, 1482
BseRI GAGGAG 3 cut(s) 299, 458, 1629
BseSI GKGCMC 1 cut(s) 2257
BseXI GCAGC 5 cut(s) 1142, 1717, 1816, 2761, 2764
BsgI GTGCAG 3 cut(s) 720, 741, 2127
Bsh1236I CGCG 2 cut(s) 1562, 1795
BshFI GGCC 8 cut(s) 91, 767, 1035, 1106, 1958, 2664, 2745, 2865
BshNI GGYRCC 3 cut(s) 689, 775, 2081
BsiHKAI GWGCWC 4 cut(s) 70, 466, 1131, 2257
BsiHKCI CYCGRG 2 cut(s) 63, 2867
BsiSI CCGG 4 cut(s) 2553, 2661, 2897, 2972
BslFI GGGAC 6 cut(s) 126, 1514, 1948, 2222, 2642, 2980
BslI CCNNNNNNNGG 2 cut(s) 623, 878
BsmAI GTCTC 3 cut(s) 1893, 2378, 2528
BsmFI GGGAC 6 cut(s) 126, 1514, 1948, 2222, 2642, 2980
BsmI GAATGC 6 cut(s) 618, 728, 1034, 1157, 1728, 2966
BsnI GGCC 8 cut(s) 91, 767, 1035, 1106, 1958, 2664, 2745, 2865
BsoBI CYCGRG 2 cut(s) 63, 2867
Bsp1286I GDGCHC 5 cut(s) 70, 466, 1131, 2257, 2717
Bsp143I GATC 9 cut(s) 138, 205, 829, 1426, 1501, 1678, 2032, 2230, 2293
Bsp19I CCATGG 2 cut(s) 73, 1092
BspACI CCGC 7 cut(s) 145, 191, 1032, 2121, 2622, 2861, 2964
BspANI GGCC 8 cut(s) 91, 767, 1035, 1106, 1958, 2664, 2745, 2865
BspCNI CTCAG 1 cut(s) 2209
BspFNI CGCG 2 cut(s) 1562, 1795
BspHI TCATGA 2 cut(s) 1372, 2709
BspLI GGNNCC 5 cut(s) 691, 777, 1071, 2083, 2920
BspMAI CTGCAG 1 cut(s) 1160
BspPI GGATC 2 cut(s) 213, 824
BspT107I GGYRCC 3 cut(s) 689, 775, 2081
BsrDI GCAATG 3 cut(s) 227, 2130, 2563
BsrFI RCCGGY 1 cut(s) 2552
BsrI ACTGG 5 cut(s) 264, 548, 845, 988, 1482
BssAI RCCGGY 1 cut(s) 2552
BssMI GATC 9 cut(s) 138, 205, 829, 1426, 1501, 1678, 2032, 2230, 2293
BssNI GRCGYC 1 cut(s) 2969
BssT1I CCWWGG 3 cut(s) 73, 1092, 2636
Bst4CI ACNGT 4 cut(s) 551, 603, 742, 2165
Bst6I CTCTTC 2 cut(s) 123, 435
BstACI GRCGYC 1 cut(s) 2969
BstBAI YACGTR 1 cut(s) 2381
BstC8I GCNNGC 5 cut(s) 1240, 1618, 1850, 2554, 2863
BstDEI CTNAG 1 cut(s) 2217
BstDSI CCRYGG 4 cut(s) 73, 272, 1092, 1107
BstF5I GGATG 9 cut(s) 139, 295, 669, 781, 897, 1056, 1318, 1585, 1618
BstFNI CGCG 2 cut(s) 1562, 1795
BstHHI GCGC 1 cut(s) 1564
BstKTI GATC 9 cut(s) 141, 208, 832, 1429, 1504, 1681, 2035, 2233, 2296
BstMAI GTCTC 3 cut(s) 1893, 2378, 2528
BstMBI GATC 9 cut(s) 138, 205, 829, 1426, 1501, 1678, 2032, 2230, 2293
BstMWI GCNNNNNNNGC 7 cut(s) 97, 698, 764, 1650, 1725, 2783, 3011
BstNSI RCATGY 3 cut(s) 1135, 1705, 2581
BstSFI CTRYAG 3 cut(s) 786, 876, 1156
BstSLI GKGCMC 1 cut(s) 2257
BstSNI TACGTA 1 cut(s) 2381
BstUI CGCG 2 cut(s) 1562, 1795
BstV1I GCAGC 5 cut(s) 1142, 1717, 1816, 2761, 2764
BstV2I GAAGAC 1 cut(s) 1740
BstX2I RGATCY 1 cut(s) 138
BstXI CCANNNNNNTGG 2 cut(s) 333, 937
BstYI RGATCY 1 cut(s) 138
BsuI GTATCC 1 cut(s) 823
BsuRI GGCC 8 cut(s) 91, 767, 1035, 1106, 1958, 2664, 2745, 2865
BtgI CCRYGG 4 cut(s) 73, 272, 1092, 1107
BtrI CACGTC 2 cut(s) 1942, 1973
BtsCI GGATG 9 cut(s) 139, 295, 669, 781, 897, 1056, 1318, 1585, 1618
BtsI GCAGTG 2 cut(s) 383, 679
BtsIMutI CAGTG 4 cut(s) 383, 541, 679, 1382
Cac8I GCNNGC 5 cut(s) 1240, 1618, 1850, 2554, 2863
CciI TCATGA 2 cut(s) 1372, 2709
CfoI GCGC 1 cut(s) 1564
Cfr10I RCCGGY 1 cut(s) 2552
Cfr13I GGNCC 6 cut(s) 89, 269, 323, 336, 2663, 2863
CseI GACGC 4 cut(s) 1568, 2927, 2977, 2994
Csp6I GTAC 4 cut(s) 776, 1253, 2082, 2589
CviQI GTAC 4 cut(s) 776, 1253, 2082, 2589
DdeI CTNAG 1 cut(s) 2217
DpnI GATC 9 cut(s) 140, 207, 831, 1428, 1503, 1680, 2034, 2232, 2295
DpnII GATC 9 cut(s) 138, 205, 829, 1426, 1501, 1678, 2032, 2230, 2293
DraI TTTAAA 1 cut(s) 166
DraIII CACNNNGTG 1 cut(s) 2252
EaeI YGGCCR 4 cut(s) 765, 1033, 1104, 1956
Eam1104I CTCTTC 2 cut(s) 123, 435
EarI CTCTTC 2 cut(s) 123, 435
Ecl136II GAGCTC 1 cut(s) 68
Eco105I TACGTA 1 cut(s) 2381
Eco130I CCWWGG 3 cut(s) 73, 1092, 2636
Eco24I GRGCYC 2 cut(s) 70, 2717
Eco32I GATATC 1 cut(s) 1140
Eco47I GGWCC 3 cut(s) 269, 323, 336
Eco53kI GAGCTC 1 cut(s) 68
Eco57I CTGAAG 5 cut(s) 15, 147, 823, 1284, 2938
Eco88I CYCGRG 2 cut(s) 63, 2867
EcoICRI GAGCTC 1 cut(s) 68
EcoRI GAATTC 3 cut(s) 1327, 2044, 2305
EcoRV GATATC 1 cut(s) 1140
EcoT14I CCWWGG 3 cut(s) 73, 1092, 2636
EcoT22I ATGCAT 2 cut(s) 1056, 2471
EcoT38I GRGCYC 2 cut(s) 70, 2717
ErhI CCWWGG 3 cut(s) 73, 1092, 2636
FalI AAGNNNNNCTT 4 cut(s) 2001, 2033, 3019, 3051
FaqI GGGAC 6 cut(s) 126, 1514, 1948, 2222, 2642, 2980
FauI CCCGC 2 cut(s) 2854, 2957
FauNDI CATATG 1 cut(s) 2465
FbaI TGATCA 1 cut(s) 2032
Fnu4HI GCNGC 6 cut(s) 1033, 1156, 1706, 1830, 2775, 2778
FokI GGATG 9 cut(s) 146, 302, 676, 768, 884, 1063, 1305, 1592, 1625
FriOI GRGCYC 2 cut(s) 70, 2717
Fsp4HI GCNGC 6 cut(s) 1033, 1156, 1706, 1830, 2775, 2778
FspBI CTAG 7 cut(s) 84, 1025, 1197, 1526, 1976, 2358, 2667
GlaI GCGC 1 cut(s) 1563
GluI GCNGC 6 cut(s) 1033, 1156, 1706, 1830, 2775, 2778
GsuI CTGGAG 3 cut(s) 984, 1416, 2286
HaeIII GGCC 8 cut(s) 91, 767, 1035, 1106, 1958, 2664, 2745, 2865
HapII CCGG 4 cut(s) 2553, 2661, 2897, 2972
HgaI GACGC 4 cut(s) 1568, 2927, 2977, 2994
HhaI GCGC 1 cut(s) 1564
Hin1I GRCGYC 1 cut(s) 2969
Hin6I GCGC 1 cut(s) 1562
HinP1I GCGC 1 cut(s) 1562
HindIII AAGCTT 4 cut(s) 835, 1651, 2386, 3019
HpaII CCGG 4 cut(s) 2553, 2661, 2897, 2972
HphI GGTGA 5 cut(s) 20, 56, 322, 1779, 2555
Hpy166II GTNNAC 3 cut(s) 339, 593, 2255
Hpy8I GTNNAC 3 cut(s) 339, 593, 2255
Hpy99I CGWCG 1 cut(s) 2943
HpyCH4III ACNGT 4 cut(s) 551, 603, 742, 2165
HpyCH4IV ACGT 5 cut(s) 364, 1941, 1972, 2281, 2380
HpyF10VI GCNNNNNNNGC 7 cut(s) 97, 698, 764, 1650, 1725, 2783, 3011
HpyF3I CTNAG 1 cut(s) 2217
HpySE526I ACGT 5 cut(s) 364, 1941, 1972, 2281, 2380
Hsp92I GRCGYC 1 cut(s) 2969
HspAI GCGC 1 cut(s) 1562
KpnI GGTACC 2 cut(s) 779, 2085
KroI GCCGGC 1 cut(s) 2552
KroNI GCCGGC 1 cut(s) 2554
Ksp22I TGATCA 1 cut(s) 2032
Kzo9I GATC 9 cut(s) 138, 205, 829, 1426, 1501, 1678, 2032, 2230, 2293
LmnI GCTCC 4 cut(s) 932, 965, 1075, 2935
Lsp1109I GCAGC 5 cut(s) 1142, 1717, 1816, 2761, 2764
LweI GCATC 9 cut(s) 654, 874, 949, 1041, 1603, 1801, 2041, 2203, 2725
MaeI CTAG 7 cut(s) 84, 1025, 1197, 1526, 1976, 2358, 2667
MaeII ACGT 5 cut(s) 364, 1941, 1972, 2281, 2380
MaeIII GTNAC 5 cut(s) 44, 1005, 1438, 1942, 2282
MalI GATC 9 cut(s) 140, 207, 831, 1428, 1503, 1680, 2034, 2232, 2295
MboI GATC 9 cut(s) 138, 205, 829, 1426, 1501, 1678, 2032, 2230, 2293
MfeI CAATTG 1 cut(s) 2688
MflI RGATCY 1 cut(s) 138
MhlI GDGCHC 5 cut(s) 70, 466, 1131, 2257, 2717
MlsI TGGCCA 2 cut(s) 767, 1958
MluNI TGGCCA 2 cut(s) 767, 1958
MlyI GAGTC 1 cut(s) 1484
MmeI TCCRAC 4 cut(s) 549, 604, 2218, 3056
Mox20I TGGCCA 2 cut(s) 767, 1958
Mph1103I ATGCAT 2 cut(s) 1056, 2471
MroNI GCCGGC 1 cut(s) 2552
MroXI GAANNNNTTC 1 cut(s) 19
MscI TGGCCA 2 cut(s) 767, 1958
MseI TTAA 7 cut(s) 165, 405, 992, 1046, 1119, 2451, 2598
MslI CAYNNNNRTG 3 cut(s) 1027, 1106, 2123
Msp20I TGGCCA 2 cut(s) 767, 1958
MspA1I CMGCKG 1 cut(s) 1738
MspI CCGG 4 cut(s) 2553, 2661, 2897, 2972
MunI CAATTG 1 cut(s) 2688
Mva1269I GAATGC 6 cut(s) 618, 728, 1034, 1157, 1728, 2966
MvnI CGCG 2 cut(s) 1562, 1795
MwoI GCNNNNNNNGC 7 cut(s) 97, 698, 764, 1650, 1725, 2783, 3011
NaeI GCCGGC 1 cut(s) 2554
NciI CCSGG 3 cut(s) 2661, 2898, 2973
NcoI CCATGG 2 cut(s) 73, 1092
NdeI CATATG 1 cut(s) 2465
NdeII GATC 9 cut(s) 138, 205, 829, 1426, 1501, 1678, 2032, 2230, 2293
NgoMIV GCCGGC 1 cut(s) 2552
NlaIV GGNNCC 5 cut(s) 691, 777, 1071, 2083, 2920
NmeAIII GCCGAG 1 cut(s) 1704
NmuCI GTSAC 3 cut(s) 44, 1438, 1942
NsiI ATGCAT 2 cut(s) 1056, 2471
NspI RCATGY 3 cut(s) 1135, 1705, 2581
PaeR7I CTCGAG 2 cut(s) 63, 2867
PagI TCATGA 2 cut(s) 1372, 2709
PasI CCCWGGG 1 cut(s) 2717
PciI ACATGT 1 cut(s) 1131
PcsI WCGNNNNNNNCGW 1 cut(s) 2955
PctI GAATGC 6 cut(s) 618, 728, 1034, 1157, 1728, 2966
PdiI GCCGGC 1 cut(s) 2554
PdmI GAANNNNTTC 1 cut(s) 19
PfoI TCCNGGA 1 cut(s) 2832
PkrI GCNGC 6 cut(s) 1034, 1157, 1707, 1831, 2776, 2779
PleI GAGTC 1 cut(s) 1483
PpsI GAGTC 1 cut(s) 1483
Ppu21I YACGTR 1 cut(s) 2381
PscI ACATGT 1 cut(s) 1131
Psp124BI GAGCTC 1 cut(s) 70
PspN4I GGNNCC 5 cut(s) 691, 777, 1071, 2083, 2920
PspPI GGNCC 6 cut(s) 89, 269, 323, 336, 2663, 2863
PspXI VCTCGAGB 1 cut(s) 63
PsrI GAACNNNNNNTAC 2 cut(s) 7, 39
PstI CTGCAG 1 cut(s) 1160
PsuI RGATCY 1 cut(s) 138
PvuII CAGCTG 1 cut(s) 1738
RsaI GTAC 4 cut(s) 777, 1254, 2083, 2590
RsaNI GTAC 4 cut(s) 776, 1253, 2082, 2589
RseI CAYNNNNRTG 3 cut(s) 1027, 1106, 2123
SacI GAGCTC 1 cut(s) 70
SaqAI TTAA 7 cut(s) 165, 405, 992, 1046, 1119, 2451, 2598
SatI GCNGC 6 cut(s) 1033, 1156, 1706, 1830, 2775, 2778
Sau3AI GATC 9 cut(s) 138, 205, 829, 1426, 1501, 1678, 2032, 2230, 2293
Sau96I GGNCC 6 cut(s) 89, 269, 323, 336, 2663, 2863
ScaI AGTACT 1 cut(s) 1254
SchI GAGTC 1 cut(s) 1484
SduI GDGCHC 5 cut(s) 70, 466, 1131, 2257, 2717
SfaNI GCATC 9 cut(s) 654, 874, 949, 1041, 1603, 1801, 2041, 2203, 2725
SfcI CTRYAG 3 cut(s) 786, 876, 1156
Sfr274I CTCGAG 2 cut(s) 63, 2867
SinI GGWCC 3 cut(s) 269, 323, 336
SlaI CTCGAG 2 cut(s) 63, 2867
SmiMI CAYNNNNRTG 3 cut(s) 1027, 1106, 2123
SmlI CTYRAG 4 cut(s) 63, 1356, 2867, 3025
SmoI CTYRAG 4 cut(s) 63, 1356, 2867, 3025
SnaBI TACGTA 1 cut(s) 2381
SpeI ACTAGT 2 cut(s) 83, 1525
SsiI CCGC 7 cut(s) 145, 191, 1032, 2121, 2622, 2861, 2964
SspMI CTAG 7 cut(s) 84, 1025, 1197, 1526, 1976, 2358, 2667
SstI GAGCTC 1 cut(s) 70
StyI CCWWGG 3 cut(s) 73, 1092, 2636
TaaI ACNGT 4 cut(s) 551, 603, 742, 2165
TaiI ACGT 5 cut(s) 367, 1944, 1975, 2284, 2383
TaqI TCGA 5 cut(s) 64, 971, 2610, 2868, 2949
TaqII GACCGA 1 cut(s) 2865
TatI WGTACW 1 cut(s) 1252
TauI GCSGC 1 cut(s) 1035
Tru1I TTAA 7 cut(s) 165, 405, 992, 1046, 1119, 2451, 2598
Tru9I TTAA 7 cut(s) 165, 405, 992, 1046, 1119, 2451, 2598
TscAI CASTG 4 cut(s) 383, 548, 679, 1389
TseFI GTSAC 3 cut(s) 44, 1438, 1942
TseI GCWGC 5 cut(s) 1155, 1705, 1829, 2774, 2777
Tsp45I GTSAC 3 cut(s) 44, 1438, 1942
TspGWI ACGGA 2 cut(s) 261, 1062
TspRI CASTG 4 cut(s) 383, 548, 679, 1389
VneI GTGCAC 1 cut(s) 2253
VpaK11BI GGWCC 3 cut(s) 269, 323, 336
XapI RAATTY 8 cut(s) 19, 714, 794, 946, 994, 1327, 2044, 2305
XbaI TCTAGA 2 cut(s) 1196, 1975
XceI RCATGY 3 cut(s) 1135, 1705, 2581
XcmI CCANNNNNNNNNTGG 2 cut(s) 1099, 1776
XhoI CTCGAG 2 cut(s) 63, 2867
XmnI GAANNNNTTC 1 cut(s) 19
XspI CTAG 7 cut(s) 84, 1025, 1197, 1526, 1976, 2358, 2667
ZrmI AGTACT 1 cut(s) 1254
Zsp2I ATGCAT 2 cut(s) 1056, 2471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.