MD08G1118900.v1.1

Subunit of clathrin-associated adaptor protein complex that plays a role in protein sorting in the late-Golgi trans-Golgi network (TGN) and or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0010492
Physical Location & Seq
Reverse (-)
10840839 .. 10846838
6000 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1118900.v1.1.491

Sequence Viewer

Length: 2451 bp
ATGGCTCCTCCGGCGCAGGCTCAGCGATCTTCCTCGCCGTCTCAGCCTTCCGGAAAAGGCGAAGTTGCTGATGTGAAATCGCAGCTCCGGAATCTTGCTGGCAGCCGAGCTCCGGGAGTTGATGATTCAAAGAGGGAGCTTTTCAAGAAGGTTATCTCTTACATGACGATTGGCATTGATGTCTCGTCTGTTTTTGGAGAGATGGTGATGTGCTCAGCAACGTCGGATATAGTTTTGAAGAAAATGTGTTACCTTTATGTTGGGAATTACGCAAAGGTCAATCCGGACCTTGCACTTTTGACGATCAATTTTCTTCAAAGAGATTGCAAGGATGAGGACCCGATGATCCGAGGGCTTGCATTGAGGAGCTTGTGTTCACTACGAGTGACAAACCTCGTGGAGTATTTGGTGGGGCCCTTAGGTGCAGGTTTGAAGGACAATAATAGTTATGTGAGGATGATAGCAGTTATGGGGGTTCTGAAGTTATATCATATATCGGCCTCAACTTGTGTTGATGCAGATTTTCCAACAACGCTTAAGCATTTGTTGCTTAACGACCGAGATACTCAGGTGGTTGCAAATTGTTTGTCTGCCCTACAAGAGATTTGGAGTTTAGAAGGAAGTGCGTCTGAAGAAGTATCCAGGGAAAGAGAAATTTTGCTTAGCAAGCCAGTTATATACTATCTTTTGAACCGGATTAGGGAGTTTAGTGAATGGGCACAGTGTCTGGTGCTTGAGCTGGTAGCTAAGTATGTACCTGCAGATCCCAATGAGATATTTGATGTCATGAATCTGCTCGAGGATAGACTTCAGCATGCAAATGGTGCTGTTGTCTTGGCAACCACTAAAGTATTTCTTCAATTGACTCTGTCAATGACTGATGTTCATCAGCAGGTATATGAACGCATTAAAGCTCCTCTCCTGACCTTAGTGAGTTCAGGAAGTCCAGAGCAATCTTATGCGGTTCTAAGCCATCTGCATCTTTTGGTGATGCGTGCACCTTTCATATTTTCTTCAGACTATAAACACTTTTATTGCCAGTACAATGAGCCATCTTATGTTAAAAAGTTGAAGCTTGAGATGTTGACTGCGGTGGCAAATGAGAGTAACACTTATGAAATTGTGACGGAATTATGTGAATATGCTGCAAATGTTGATATTCCCATTGCAAGAGAGTCGATTCGTGCAGTTGGGAAAATAGCTCTACAACAGTATGACGTGAATGCAATAGTTGACCGACTTCTTCAATTTCTGGAGATGGAAAAGGACTATGTAACTGCTGAAGCTCTGGTTCTTGTAAAAGATCTGCTGAGGAAATATCCACAATGGAGTCAGGATTGTATTGCGGTTGTTGGGAACATCAGCAGCAAAAATGTTCAAGAACCCAAGGCCAAGGCAGCTCTTATATGGATGCTGGGGGAGTATTCTCACGAAATGCATGATGCACCTTATATTTTAGAGGGTTTGATTGAAAATTGGGAAGATGAGCATTCCGCTGAGGTTCGTTTACATCTACTCACAGCAGTAATGAAGTGTTTTTTTAAGAGGCCGCCTGAGACTCAAAAATCCTTGGGAGCTGCACTGGCTGCAGGTCTTGCTGATTTTCATCAGGATGTTCATGATCGAGCCTTATTCTACTACAGGCTTTTGCAATATAACATAAGTGTAGCAGAACGAGTGGTGAACCCTCCAAAGCAAGCTGTTTCTGTCTTTGCTGATACCCAGAGCAGTGAAATCAAAGATCGGATATTCGACGAATTTAACAGTTTGTCTGTGGTATATCAAAAGCCATCCTACATGTTTACTTATAAGGAACACAGAGGACCGTTTGAGTTTTCAGATGAAATTGGAAATGTATCAATTGGGACAGAATCTACAGATACTGTTGTTCCTCATAGAGTCGAAGCTAATGATAAAGATCTGCTATTAAGTACTTCAGAGAAGGAGGAGACAAGAGGTCTCAATAATAGTTCTTATGCATATAGTGCTCCTTCATACGATGCTTCGTCTTTGTCTGTTCCCACTTCTCAACTGTCGGAGTTGGCAATTTCAAACCCTAGCGTGTCTGGAAATGTTCCGCAGTCTAGCTTTGCAATTGATGATCTCCTTGGCTTGGGACTACCTACTGCACCTGCACCTGCACCTTCACCTCCTCCATTGAAGCTTAATCCAAAAGCTGTCTTAGATCCAACAACTTTTCAGCAGAAGTGGCGCCAACTGCCGATATCTTTATCACAGGAGTATTCTATTAATCCTCAAGGAGTTGCAGCATTGACAACGCCTCAAGCTCTCCAACGGCATATGCAAGGCCAATCCATCCACTGCATTGCATCCGGTGGTCAATCCCCCAACTTCAAGTTTTTCTTCTTCGCACAAAAGGCAGAAGAATCTTCTACATTTTTGATAGTGTATAGTCAACACATCATCCGCCAAAGCTCAAATAAAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0019899 GO:0019900 GO:0019901 GO:0043424
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Adaptin_N B2-adapt-app_C

Protein Analysis

817

Amino Acids

90.95

Weight (kDa)

5.51

Isoelectric Point (pI)

44.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adaptin_N PF01602 30 - 551 1.3e-93 Adaptin N terminal region
Cnd1 PF12717 115 - 287 5.3e-44 non-SMC mitotic condensation complex subunit 1
B2-adapt-app_C PF09066 724 - 802 5.5e-16 Beta2-adaptin appendage, C-terminal sub-domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010492)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G11490 AT5G11490 AT5G11490
fragaria_vesca FvH4_2g36170 FvH4_2g36170 FvH4_2g36170
malus_domestica MD08G1118900.v1.1 MD15G1098200.v1.1
prunus_persica Prupe.1G453100_v2.0.a1
pyrus_communis pycom08g09930 pycom15g09000
rosa_chinensis RchiOBHm_Chr6g0305701
rosa_laevigata RLG00000010867
rosa_multiflora Rmu_sc0001809.1_g000035
rosa_roxburghii Rroxscaffold_7G00162430
rosa_rugosa Rorug06G0343300
rosa_samantha Rh6AG454800 Rh6BG446700 Rh6CG468700 Rh6DG455300
rosa_wichuraiana Rw6G039660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1809
AarI CACCTGC 2 cut(s) 2140, 2146
Acc36I ACCTGC 6 cut(s) 416, 766, 883, 1580, 2140, 2146
AccB1I GGYRCC 1 cut(s) 2211
AccIII TCCGGA 3 cut(s) 50, 87, 283
AciI CCGC 7 cut(s) 962, 1091, 1346, 1494, 1550, 2078, 2428
AclWI GGATC 3 cut(s) 340, 758, 2180
AcsI RAATTY 2 cut(s) 654, 1757
AcuI CTGAAG 6 cut(s) 500, 651, 794, 999, 1302, 1920
AcyI GRCGYC 1 cut(s) 2212
AfaI GTAC 3 cut(s) 756, 1043, 1933
AfiI CCNNNNNNNGG 3 cut(s) 112, 700, 2113
AflII CTTAAG 1 cut(s) 536
AflIII ACRYGT 1 cut(s) 1797
AhdI GACNNNNNGTC 1 cut(s) 1956
AjiI CACGTC 1 cut(s) 1219
AjnI CCWGG 1 cut(s) 641
AloI GAACNNNNNNTCC 2 cut(s) 358, 390
Alw21I GWGCWC 4 cut(s) 112, 215, 1000, 1990
Alw26I GTCTC 5 cut(s) 45, 187, 1550, 1943, 1964
Alw44I GTGCAC 1 cut(s) 996
AlwI GGATC 3 cut(s) 340, 758, 2180
AlwNI CAGNNNCTG 2 cut(s) 727, 1883
Ama87I CYCGRG 1 cut(s) 797
Aor13HI TCCGGA 3 cut(s) 50, 87, 283
AoxI GGCC 5 cut(s) 413, 498, 1389, 1547, 2308
ApaI GGGCCC 1 cut(s) 417
ApaLI GTGCAC 1 cut(s) 996
ApeKI GCWGC 8 cut(s) 82, 102, 1145, 1365, 1397, 1577, 1586, 2267
ApoI RAATTY 2 cut(s) 654, 1757
ArsI GACNNNNNNTTYG 2 cut(s) 572, 604
AseI ATTAAT 1 cut(s) 2250
AspLEI GCGC 2 cut(s) 16, 2214
AspS9I GGNCC 5 cut(s) 286, 337, 413, 414, 1823
AsuC2I CCSGG 1 cut(s) 114
AsuHPI GGTGA 4 cut(s) 217, 1000, 1693, 2139
AvaI CYCGRG 1 cut(s) 797
AvaII GGWCC 3 cut(s) 286, 337, 1823
AxyI CCTNAGG 1 cut(s) 418
BaeGI GKGCMC 3 cut(s) 417, 721, 1000
BaeI ACNNNNGTAYC 4 cut(s) 555, 588, 1872, 1905
BanI GGYRCC 1 cut(s) 2211
BanII GRGCYC 2 cut(s) 112, 417
BauI CACGAG 1 cut(s) 395
Bbv12I GWGCWC 4 cut(s) 112, 215, 1000, 1990
BbvCI CCTCAGC 2 cut(s) 1310, 1497
BbvI GCAGC 8 cut(s) 94, 114, 1132, 1377, 1409, 1564, 1573, 2279
BccI CCATC 6 cut(s) 196, 981, 1060, 1252, 1798, 2324
BceAI ACGGC 2 cut(s) 22, 2312
BcgI CGANNNNNNTGC 2 cut(s) 1158, 1192
BciT130I CCWGG 1 cut(s) 643
BciVI GTATCC 1 cut(s) 649
BcnI CCSGG 1 cut(s) 114
BcoDI GTCTC 5 cut(s) 45, 187, 1550, 1943, 1964
BfaI CTAG 2 cut(s) 2058, 2085
BfmI CTRYAG 4 cut(s) 759, 1587, 1639, 1875
BfoI RGCGCY 1 cut(s) 2215
BfrI CTTAAG 1 cut(s) 536
BfuAI ACCTGC 6 cut(s) 416, 766, 883, 1580, 2140, 2146
BfuI GTATCC 1 cut(s) 649
BglII AGATCT 2 cut(s) 1303, 1918
BisI GCNGC 9 cut(s) 83, 103, 1146, 1366, 1398, 1550, 1578, 1587, 2268
BlpI GCTNAGC 3 cut(s) 21, 214, 662
BlsI GCNGC 9 cut(s) 84, 104, 1147, 1367, 1399, 1551, 1579, 1588, 2269
BmcAI AGTACT 1 cut(s) 1933
Bme1390I CCNGG 2 cut(s) 114, 643
Bme18I GGWCC 3 cut(s) 286, 337, 1823
BmeRI GACNNNNNGTC 1 cut(s) 1956
BmeT110I CYCGRG 1 cut(s) 797
BmgBI CACGTC 1 cut(s) 1219
BmgT120I GGNCC 5 cut(s) 286, 337, 413, 414, 1823
BmiI GGNNCC 5 cut(s) 6, 339, 414, 415, 2213
BmrFI CCNGG 2 cut(s) 114, 643
BmsI GCATC 7 cut(s) 505, 981, 988, 1401, 1432, 1990, 2339
BpmI CTGGAG 1 cut(s) 1274
Bpu10I CCTNAGC 2 cut(s) 1310, 1497
Bpu1102I GCTNAGC 3 cut(s) 21, 214, 662
BpuEI CTTGAG 4 cut(s) 755, 1097, 2241, 2268
BpuMI CCSGG 1 cut(s) 114
BsaBI GATNNNNATC 3 cut(s) 885, 1605, 1917
BsaHI GRCGYC 1 cut(s) 2212
BsaI GGTCTC 1 cut(s) 1964
BsaJI CCNNGG 6 cut(s) 349, 642, 1386, 1392, 1569, 2107
BsaWI WCCGGW 5 cut(s) 50, 87, 283, 693, 2333
BsaXI ACNNNNNCTCC 4 cut(s) 189, 219, 358, 388
Bsc4I CCNNNNNNNGG 3 cut(s) 112, 700, 2113
Bse1I ACTGG 3 cut(s) 671, 1039, 1587
Bse21I CCTNAGG 1 cut(s) 418
Bse3DI GCAATG 2 cut(s) 1164, 2325
Bse8I GATNNNNATC 3 cut(s) 885, 1605, 1917
BseAI TCCGGA 3 cut(s) 50, 87, 283
BseBI CCWGG 1 cut(s) 643
BseDI CCNNGG 6 cut(s) 349, 642, 1386, 1392, 1569, 2107
BseGI GGATG 8 cut(s) 337, 462, 1416, 1618, 1790, 2316, 2330, 2424
BseJI GATNNNNATC 3 cut(s) 885, 1605, 1917
BseLI CCNNNNNNNGG 3 cut(s) 112, 700, 2113
BseMI GCAATG 2 cut(s) 1164, 2325
BseMII CTCAG 7 cut(s) 35, 56, 228, 581, 1301, 1488, 1545
BseNI ACTGG 3 cut(s) 671, 1039, 1587
BseRI GAGGAG 4 cut(s) 379, 906, 1961, 2142
BseSI GKGCMC 3 cut(s) 417, 721, 1000
BseXI GCAGC 8 cut(s) 94, 114, 1132, 1377, 1409, 1564, 1573, 2279
BseYI CCCAGC 1 cut(s) 1414
BsgI GTGCAG 6 cut(s) 444, 1206, 1563, 2112, 2118, 2124
Bsh1285I CGRYCG 1 cut(s) 559
BshFI GGCC 5 cut(s) 415, 500, 1391, 1549, 2310
BshNI GGYRCC 1 cut(s) 2211
BsiEI CGRYCG 1 cut(s) 559
BsiHKAI GWGCWC 4 cut(s) 112, 215, 1000, 1990
BsiHKCI CYCGRG 1 cut(s) 797
BsiSI CCGG 7 cut(s) 11, 51, 88, 113, 284, 694, 2334
BslFI GGGAC 2 cut(s) 1879, 2130
BslI CCNNNNNNNGG 3 cut(s) 112, 700, 2113
BsmAI GTCTC 5 cut(s) 45, 187, 1550, 1943, 1964
BsmBI CGTCTC 1 cut(s) 45
BsmFI GGGAC 2 cut(s) 1879, 2130
BsmI GAATGC 2 cut(s) 1228, 1489
BsnI GGCC 5 cut(s) 415, 500, 1391, 1549, 2310
Bso31I GGTCTC 1 cut(s) 1964
BsoBI CYCGRG 1 cut(s) 797
Bsp120I GGGCCC 1 cut(s) 413
Bsp1286I GDGCHC 6 cut(s) 112, 215, 417, 721, 1000, 1990
Bsp13I TCCGGA 3 cut(s) 50, 87, 283
Bsp1720I GCTNAGC 3 cut(s) 21, 214, 662
BspACI CCGC 7 cut(s) 962, 1091, 1346, 1494, 1550, 2078, 2428
BspANI GGCC 5 cut(s) 415, 500, 1391, 1549, 2310
BspCNI CTCAG 7 cut(s) 34, 55, 227, 580, 1302, 1489, 1546
BspEI TCCGGA 3 cut(s) 50, 87, 283
BspHI TCATGA 2 cut(s) 786, 1618
BspLI GGNNCC 5 cut(s) 6, 339, 414, 415, 2213
BspMAI CTGCAG 2 cut(s) 763, 1591
BspMI ACCTGC 6 cut(s) 416, 766, 883, 1580, 2140, 2146
BspPI GGATC 3 cut(s) 340, 758, 2180
BspT107I GGYRCC 1 cut(s) 2211
BspTI CTTAAG 1 cut(s) 536
BspTNI GGTCTC 1 cut(s) 1964
BsrDI GCAATG 2 cut(s) 1164, 2325
BsrI ACTGG 3 cut(s) 671, 1039, 1587
BssECI CCNNGG 6 cut(s) 349, 642, 1386, 1392, 1569, 2107
BssNI GRCGYC 1 cut(s) 2212
BssSI CACGAG 1 cut(s) 395
BssT1I CCWWGG 4 cut(s) 1386, 1392, 1569, 2107
Bst2BI CACGAG 1 cut(s) 395
Bst2UI CCWGG 1 cut(s) 643
Bst4CI ACNGT 6 cut(s) 723, 1212, 1766, 1827, 1885, 2034
BstACI GRCGYC 1 cut(s) 2212
BstAFI CTTAAG 1 cut(s) 536
BstAPI GCANNNNNTGC 5 cut(s) 547, 824, 1586, 1595, 1985
BstC8I GCNNGC 7 cut(s) 18, 100, 357, 668, 816, 996, 1698
BstF5I GGATG 8 cut(s) 337, 462, 1416, 1618, 1790, 2316, 2330, 2424
BstH2I RGCGCY 1 cut(s) 2215
BstHHI GCGC 2 cut(s) 16, 2214
BstMAI GTCTC 5 cut(s) 45, 187, 1550, 1943, 1964
BstMCI CGRYCG 1 cut(s) 559
BstNI CCWGG 1 cut(s) 643
BstNSI RCATGY 2 cut(s) 818, 1801
BstSCI CCNGG 2 cut(s) 112, 641
BstSFI CTRYAG 4 cut(s) 759, 1587, 1639, 1875
BstSLI GKGCMC 3 cut(s) 417, 721, 1000
BstV1I GCAGC 8 cut(s) 94, 114, 1132, 1377, 1409, 1564, 1573, 2279
BstX2I RGATCY 4 cut(s) 763, 1303, 1918, 2185
BstYI RGATCY 4 cut(s) 763, 1303, 1918, 2185
Bsu36I CCTNAGG 1 cut(s) 418
BsuI GTATCC 1 cut(s) 649
BsuRI GGCC 5 cut(s) 415, 500, 1391, 1549, 2310
BtrI CACGTC 1 cut(s) 1219
BtsCI GGATG 8 cut(s) 337, 462, 1416, 1618, 1790, 2316, 2330, 2424
BtsI GCAGTG 2 cut(s) 1735, 2320
BtsIMutI CAGTG 4 cut(s) 728, 1580, 1735, 2320
BveI ACCTGC 6 cut(s) 416, 766, 883, 1580, 2140, 2146
Cac8I GCNNGC 7 cut(s) 18, 100, 357, 668, 816, 996, 1698
CaiI CAGNNNCTG 2 cut(s) 727, 1883
CciI TCATGA 2 cut(s) 786, 1618
CfoI GCGC 2 cut(s) 16, 2214
Cfr13I GGNCC 5 cut(s) 286, 337, 413, 414, 1823
CseI GACGC 1 cut(s) 615
Csp6I GTAC 3 cut(s) 755, 1042, 1932
CspCI CAANNNNNGTGG 2 cut(s) 378, 413
CviAII CATG 6 cut(s) 163, 787, 815, 1439, 1619, 1798
CviQI GTAC 3 cut(s) 755, 1042, 1932
DinI GGCGCC 1 cut(s) 2213
DriI GACNNNNNGTC 1 cut(s) 1956
Eam1105I GACNNNNNGTC 1 cut(s) 1956
EciI GGCGGA 1 cut(s) 2417
Ecl136II GAGCTC 1 cut(s) 110
Eco130I CCWWGG 4 cut(s) 1386, 1392, 1569, 2107
Eco24I GRGCYC 2 cut(s) 112, 417
Eco31I GGTCTC 1 cut(s) 1964
Eco32I GATATC 1 cut(s) 2226
Eco47I GGWCC 3 cut(s) 286, 337, 1823
Eco53kI GAGCTC 1 cut(s) 110
Eco57I CTGAAG 6 cut(s) 500, 651, 794, 999, 1302, 1920
Eco81I CCTNAGG 1 cut(s) 418
Eco88I CYCGRG 1 cut(s) 797
EcoICRI GAGCTC 1 cut(s) 110
EcoO109I RGGNCCY 3 cut(s) 337, 413, 414
EcoRII CCWGG 1 cut(s) 641
EcoRV GATATC 1 cut(s) 2226
EcoT14I CCWWGG 4 cut(s) 1386, 1392, 1569, 2107
EcoT22I ATGCAT 2 cut(s) 1440, 1981
EcoT38I GRGCYC 2 cut(s) 112, 417
EgeI GGCGCC 1 cut(s) 2213
EheI GGCGCC 1 cut(s) 2213
ErhI CCWWGG 4 cut(s) 1386, 1392, 1569, 2107
Esp3I CGTCTC 1 cut(s) 45
FaeI CATG 6 cut(s) 166, 790, 818, 1442, 1622, 1801
FalI AAGNNNNNCTT 4 cut(s) 840, 872, 2348, 2380
FaqI GGGAC 2 cut(s) 1879, 2130
FatI CATG 6 cut(s) 162, 786, 814, 1438, 1618, 1797
FauNDI CATATG 1 cut(s) 2301
Fnu4HI GCNGC 9 cut(s) 83, 103, 1146, 1366, 1398, 1550, 1578, 1587, 2268
FokI GGATG 8 cut(s) 344, 469, 1423, 1625, 1777, 2303, 2317, 2411
FriOI GRGCYC 2 cut(s) 112, 417
Fsp4HI GCNGC 9 cut(s) 83, 103, 1146, 1366, 1398, 1550, 1578, 1587, 2268
FspBI CTAG 2 cut(s) 2058, 2085
GlaI GCGC 2 cut(s) 15, 2213
GluI GCNGC 9 cut(s) 83, 103, 1146, 1366, 1398, 1550, 1578, 1587, 2268
GsaI CCCAGC 1 cut(s) 1418
GsuI CTGGAG 1 cut(s) 1274
HaeII RGCGCY 1 cut(s) 2215
HaeIII GGCC 5 cut(s) 415, 500, 1391, 1549, 2310
HapII CCGG 7 cut(s) 11, 51, 88, 113, 284, 694, 2334
HgaI GACGC 1 cut(s) 615
HhaI GCGC 2 cut(s) 16, 2214
Hin1I GRCGYC 1 cut(s) 2212
Hin1II CATG 6 cut(s) 166, 790, 818, 1442, 1622, 1801
Hin6I GCGC 2 cut(s) 14, 2212
HinP1I GCGC 2 cut(s) 14, 2212
HincII GTYRAC 3 cut(s) 1086, 1234, 2417
HindII GTYRAC 3 cut(s) 1086, 1234, 2417
HindIII AAGCTT 2 cut(s) 1073, 2162
HpaII CCGG 7 cut(s) 11, 51, 88, 113, 284, 694, 2334
HphI GGTGA 4 cut(s) 217, 1000, 1693, 2139
Hpy166II GTNNAC 8 cut(s) 377, 998, 1086, 1234, 1508, 1684, 1803, 2417
Hpy188I TCNGA 9 cut(s) 226, 350, 480, 631, 1018, 1746, 1840, 1939, 2038
Hpy8I GTNNAC 8 cut(s) 377, 998, 1086, 1234, 1508, 1684, 1803, 2417
Hpy99I CGWCG 2 cut(s) 226, 1757
HpyAV CCTTC 7 cut(s) 57, 142, 427, 611, 1936, 2001, 2154
HpyCH4III ACNGT 6 cut(s) 723, 1212, 1766, 1827, 1885, 2034
HpyCH4IV ACGT 2 cut(s) 221, 1218
HpySE526I ACGT 2 cut(s) 221, 1218
Hsp92I GRCGYC 1 cut(s) 2212
Hsp92II CATG 6 cut(s) 166, 790, 818, 1442, 1622, 1801
HspAI GCGC 2 cut(s) 14, 2212
KasI GGCGCC 1 cut(s) 2211
Kpn2I TCCGGA 3 cut(s) 50, 87, 283
LmnI GCTCC 8 cut(s) 10, 90, 115, 136, 366, 919, 1574, 1993
Lsp1109I GCAGC 8 cut(s) 94, 114, 1132, 1377, 1409, 1564, 1573, 2279
LweI GCATC 7 cut(s) 505, 981, 988, 1401, 1432, 1990, 2339
MaeI CTAG 2 cut(s) 2058, 2085
MaeII ACGT 2 cut(s) 221, 1218
MaeIII GTNAC 5 cut(s) 248, 385, 1106, 1123, 1273
MfeI CAATTG 3 cut(s) 860, 1860, 2094
MflI RGATCY 4 cut(s) 763, 1303, 1918, 2185
MhlI GDGCHC 6 cut(s) 112, 215, 417, 721, 1000, 1990
Mly113I GGCGCC 1 cut(s) 2212
MlyI GAGTC 5 cut(s) 859, 1184, 1339, 1552, 1908
MmeI TCCRAC 5 cut(s) 204, 551, 2016, 2213, 2317
Mph1103I ATGCAT 2 cut(s) 1440, 1981
MroI TCCGGA 3 cut(s) 50, 87, 283
MslI CAYNNNNRTG 2 cut(s) 819, 1611
MspA1I CMGCKG 1 cut(s) 1496
MspCI CTTAAG 1 cut(s) 536
MspI CCGG 7 cut(s) 11, 51, 88, 113, 284, 694, 2334
MspR9I CCNGG 2 cut(s) 114, 643
MunI CAATTG 3 cut(s) 860, 1860, 2094
Mva1269I GAATGC 2 cut(s) 1228, 1489
MvaI CCWGG 1 cut(s) 643
NarI GGCGCC 1 cut(s) 2212
NciI CCSGG 1 cut(s) 114
NdeI CATATG 1 cut(s) 2301
NlaIII CATG 6 cut(s) 166, 790, 818, 1442, 1622, 1801
NlaIV GGNNCC 5 cut(s) 6, 339, 414, 415, 2213
NmeAIII GCCGAG 1 cut(s) 131
NmuCI GTSAC 2 cut(s) 385, 1123
NsiI ATGCAT 2 cut(s) 1440, 1981
NspI RCATGY 2 cut(s) 818, 1801
PaeI GCATGC 1 cut(s) 818
PaeR7I CTCGAG 1 cut(s) 797
PagI TCATGA 2 cut(s) 786, 1618
PaqCI CACCTGC 2 cut(s) 2140, 2146
PciI ACATGT 1 cut(s) 1797
PctI GAATGC 2 cut(s) 1228, 1489
PfeI GAWTC 6 cut(s) 91, 125, 790, 1180, 1871, 2387
PflFI GACNNNGTC 1 cut(s) 868
PfoI TCCNGGA 1 cut(s) 112
PkrI GCNGC 9 cut(s) 84, 104, 1147, 1367, 1399, 1551, 1579, 1588, 2269
PleI GAGTC 5 cut(s) 859, 1183, 1338, 1552, 1907
PluTI GGCGCC 1 cut(s) 2215
PpsI GAGTC 5 cut(s) 859, 1183, 1338, 1552, 1907
PpuMI RGGWCCY 1 cut(s) 337
PscI ACATGT 1 cut(s) 1797
PshBI ATTAAT 1 cut(s) 2250
PsiI TTATAA 1 cut(s) 1809
Psp124BI GAGCTC 1 cut(s) 112
Psp5II RGGWCCY 1 cut(s) 337
Psp6I CCWGG 1 cut(s) 641
PspFI CCCAGC 1 cut(s) 1414
PspGI CCWGG 1 cut(s) 641
PspN4I GGNNCC 5 cut(s) 6, 339, 414, 415, 2213
PspOMI GGGCCC 1 cut(s) 413
PspPI GGNCC 5 cut(s) 286, 337, 413, 414, 1823
PspPPI RGGWCCY 1 cut(s) 337
PspXI VCTCGAGB 1 cut(s) 797
PstI CTGCAG 2 cut(s) 763, 1591
PstNI CAGNNNCTG 2 cut(s) 727, 1883
PsuI RGATCY 4 cut(s) 763, 1303, 1918, 2185
PsyI GACNNNGTC 1 cut(s) 868
RsaI GTAC 3 cut(s) 756, 1043, 1933
RsaNI GTAC 3 cut(s) 755, 1042, 1932
RseI CAYNNNNRTG 2 cut(s) 819, 1611
SacI GAGCTC 1 cut(s) 112
SatI GCNGC 9 cut(s) 83, 103, 1146, 1366, 1398, 1550, 1578, 1587, 2268
Sau96I GGNCC 5 cut(s) 286, 337, 413, 414, 1823
ScaI AGTACT 1 cut(s) 1933
SchI GAGTC 5 cut(s) 859, 1184, 1339, 1552, 1908
ScrFI CCNGG 2 cut(s) 114, 643
SduI GDGCHC 6 cut(s) 112, 215, 417, 721, 1000, 1990
SfaNI GCATC 7 cut(s) 505, 981, 988, 1401, 1432, 1990, 2339
SfcI CTRYAG 4 cut(s) 759, 1587, 1639, 1875
SfoI GGCGCC 1 cut(s) 2213
Sfr274I CTCGAG 1 cut(s) 797
SinI GGWCC 3 cut(s) 286, 337, 1823
SlaI CTCGAG 1 cut(s) 797
SmiMI CAYNNNNRTG 2 cut(s) 819, 1611
SmlI CTYRAG 6 cut(s) 536, 734, 797, 1076, 2256, 2283
SmoI CTYRAG 6 cut(s) 536, 734, 797, 1076, 2256, 2283
SphI GCATGC 1 cut(s) 818
SsiI CCGC 7 cut(s) 962, 1091, 1346, 1494, 1550, 2078, 2428
SspDI GGCGCC 1 cut(s) 2211
SspMI CTAG 2 cut(s) 2058, 2085
SstI GAGCTC 1 cut(s) 112
StyD4I CCNGG 2 cut(s) 112, 641
StyI CCWWGG 4 cut(s) 1386, 1392, 1569, 2107
TaaI ACNGT 6 cut(s) 723, 1212, 1766, 1827, 1885, 2034
TaiI ACGT 2 cut(s) 224, 1221
TaqI TCGA 5 cut(s) 798, 1178, 1624, 1752, 1902
TaqII GACCGA 2 cut(s) 573, 1251
TatI WGTACW 2 cut(s) 1041, 1931
TauI GCSGC 1 cut(s) 1552
TfiI GAWTC 6 cut(s) 91, 125, 790, 1180, 1871, 2387
TscAI CASTG 4 cut(s) 728, 1587, 1735, 2327
TseFI GTSAC 2 cut(s) 385, 1123
TseI GCWGC 8 cut(s) 82, 102, 1145, 1365, 1397, 1577, 1586, 2267
Tsp45I GTSAC 2 cut(s) 385, 1123
TspGWI ACGGA 1 cut(s) 1142
TspRI CASTG 4 cut(s) 728, 1587, 1735, 2327
Tth111I GACNNNGTC 1 cut(s) 868
Vha464I CTTAAG 1 cut(s) 536
VneI GTGCAC 1 cut(s) 996
VpaK11BI GGWCC 3 cut(s) 286, 337, 1823
VspI ATTAAT 1 cut(s) 2250
XapI RAATTY 2 cut(s) 654, 1757
XceI RCATGY 2 cut(s) 818, 1801
XhoI CTCGAG 1 cut(s) 797
XspI CTAG 2 cut(s) 2058, 2085
ZrmI AGTACT 1 cut(s) 1933
Zsp2I ATGCAT 2 cut(s) 1440, 1981
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.