MD15G1098200.v1.1

Subunit of clathrin-associated adaptor protein complex that plays a role in protein sorting in the late-Golgi trans-Golgi network (TGN) and or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0010492
Physical Location & Seq
Reverse (-)
6819582 .. 6825831
6250 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1098200.v1.1.491

Sequence Viewer

Length: 2529 bp
ATGGCTCCTCCGGCGCAGTCTCAGCGATCTTCCTCGCCGTCTCAGCCTGCCGGAAAAGGCGAAGTTGCTGATGTGAAATCGCAGCTCCGGAATCTTGCCGGCAGCCGAGCTCCGGGAGTTGATGATTCAAAGCGGGAGCTTTTCAAGAAGGTTATCTCTTACATGACAATTGGCATTGATGTCTCGTCTGTTTTTGGGGAGATGGTGATGTGCTCAGCCACATCGGATATAGTTTTGAAGAAAATGTGTTACCTTTATGTCGGGAATTATGCAAAGGTCAATCCGGACCTTGCACTTTTGACGATCAATTTTCTTCAAAGAGATTGCAAGGATGAGGACCCGATGATCCGGGGGCTTGCATTGAGGAGCTTGTGTTCGCTACGAGTGACAAACCTTGTAGAGTATTTGGTTGGGCCTTTAGGTGCAGGGTTGAAGGACAATAATAGTTATGTGAGGATGATAGCAGTTATGGGGGTTCTGAAGTTGTATCATATATCGGCCTCAACTTGTGTTGATGCAGATTTTCCAACAACGCTTAAGCATTTGTTGCTTAATGACCGAGATACTCAGGTGGTTGCAAATTGTTTGTCTGCCCTACAAGAGATTTGGAGCTTAGGAGGAAGTGCATCTGAAGAAGTATCCAGGGAGAGAGAAATTTTGCTTAGCAAGCCAGTCATATACTATCTTTTGAACCGGATTAGGGAATTTAGTGAATGGGCCCAATGTCTGGTACTTGAGTTGGTAGCTAAATATGTACCCGCAGATCCCAATGAGATATTTGATGTCATGAATCTGCTCGAGGATAGACTTCAGCATGCAAATGGTGCTGTTGTCTTAGCAACCACTAAAGTATTTCTTCAATTGACTCTGTCAATGACTGATGTACATCAGCAGGTATATGAACGCATTAAAGCTCCTCTCCTGACCTTAGTGAGTTCAGGAAGTCCGGAGCAATCTTATGCAGTTCTAAGCCATCTGCATCTTTTGGTGATGCGTGCACCTTTCATATTTTCTTCAGACTATAAACACTTTTATTGCCAGTACAATGAGCCATCTTATGTTAAAAAGTTGAAGCTTGAGATGTTGACTGCAGTGGCAAATGAGAGTAACACTTATGAAATTGTGACGGAATTATGTGAATATGCTGCAAATGTTGATATTCCCATTGCAAGAGAGTCAATCCGTGCAGTTGGGAAAATAGCTCTACAACAGTATGATGTGAATGCAATTGTTGATCGACTTCTTCAATTTCTGGAGATGGAAAAGGACTATGTAACTGCTGAAGCTCTGGTTCTTGTAAAAGATCTGCTGAGGAAATATCCACAATGGAGTCAGGATTGTATTGCAGTTGTTGGGAACATCAGCAGCAAAAATGTTCAAGAACCCAAGGCCAAGGCTGCTCTTATATGGATGCTGGGGGAGTATTCTCAGGAAATGCATGATGCACCTTATATTTTAGAGGGTTTGATTGAAAATTGGGAAGATGAGCATTCCGCTGAGGTTCGTTTACATCTACTAACAGCAGTGATGAAGTGTTTTTTTAAGAGGCCACCTGAGACTCAGAAATCCCTGGGAGCTGCACTGGCCGCAGGTCTTGCTGATTTTCATCAGGATGTTCATGATAGAGCCTTATTCTACTACAGGCTTTTGCAATATAAAATAAGTGTAGCAGAACAAGTGGTGAACCCTCCAAAGCAAGCTGTTTCTGTCTTTGCTGATACCCAGAGCAGTGAAATCAAAGATCGGATTTTCGACGAATTTAACAGTTTATCTGTGGTATATCAGAAGCCTTCGTACATGTTTACTTATAAGGAACACAGAGGCCCATTTGAGTTTTCAGATGAAATTGGAAATGTATCTATTGGGACAGAATCTGCGGATACTGTTGTTCCAAACAGAGTCGAAGCAAATGATAAGGATCTGCTTTTAAGTACCTCAGAGAAGGAGGAAACAAGAGGTCTCAATAACAGTTCTTCTGCATATAGTGCTCCTTCATATGATGCTTCGTCTGTGTCTGTTCCCGCTTCTCAACTGTCGGAGTTGGCAATTTCAAACCCTAGTGTGTCAGGAAATGCTCCGCAGTCTAGCTTTGCAATTGATGATCTGCTTGGCTTGGGACTAGCTGTTGCACCTGCGCCTGCACCTTCACCTCCTACGTTGAAGCTTAATCCAAAAGCTGTTTTGGATCCAACCTCTTTCCAGCAGAAGTGGCGCCAACTGCCAATATCTTTATCACAGGAGTATTCTATGAACCCTCAAGGAGTTGCAGCATTGACAACGCCTCAAGCACTCTTACGGCATATGCAAGGCCAAGCCATCCACTGCATTGCATCCGGCGGCCAATCCCCCAACTTCAAGTTTTTCTTCTTCGCACAGAAGGCAGAAGAATCCTCAACATTTTTGGTAGAGTGTATAGTCAACACATCATCCGCCAAAGCTCAAATAAAGATAAAAGCCGATGACCAAAGTGCAACTCAGCCCTTTTCATCCGCGTTCCAGTCTGCCCTGTCCAAGTTCGGCATGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0019899 GO:0019900 GO:0019901 GO:0043424
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Adaptin_N B2-adapt-app_C

Protein Analysis

843

Amino Acids

93.15

Weight (kDa)

5.45

Isoelectric Point (pI)

43.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adaptin_N PF01602 30 - 551 5.5e-94 Adaptin N terminal region
Cnd1 PF12717 115 - 287 6.3e-44 non-SMC mitotic condensation complex subunit 1
B2-adapt-app_C PF09066 724 - 836 1.4e-24 Beta2-adaptin appendage, C-terminal sub-domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010492)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G11490 AT5G11490 AT5G11490
fragaria_vesca FvH4_2g36170 FvH4_2g36170 FvH4_2g36170
malus_domestica MD08G1118900.v1.1 MD15G1098200.v1.1
prunus_persica Prupe.1G453100_v2.0.a1
pyrus_communis pycom08g09930 pycom15g09000
rosa_chinensis RchiOBHm_Chr6g0305701
rosa_laevigata RLG00000010867
rosa_multiflora Rmu_sc0001809.1_g000035
rosa_roxburghii Rroxscaffold_7G00162430
rosa_rugosa Rorug06G0343300
rosa_samantha Rh6AG454800 Rh6BG446700 Rh6CG468700 Rh6DG455300
rosa_wichuraiana Rw6G039660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1809
AarI CACCTGC 1 cut(s) 2140
Acc36I ACCTGC 3 cut(s) 883, 1580, 2140
AccB1I GGYRCC 1 cut(s) 2211
AccB7I CCANNNNNTGG 1 cut(s) 727
AccII CGCG 1 cut(s) 2492
AccIII TCCGGA 3 cut(s) 87, 283, 946
AclWI GGATC 5 cut(s) 340, 758, 1926, 2180, 2193
AcoI YGGCCR 2 cut(s) 1584, 2338
AcsI RAATTY 3 cut(s) 654, 704, 1757
AcuI CTGAAG 5 cut(s) 500, 651, 794, 999, 1302
AcyI GRCGYC 1 cut(s) 2212
AfaI GTAC 6 cut(s) 732, 756, 885, 1043, 1796, 1933
AfiI CCNNNNNNNGG 3 cut(s) 112, 700, 727
AflII CTTAAG 1 cut(s) 536
AflIII ACRYGT 1 cut(s) 1797
AjnI CCWGG 2 cut(s) 641, 1569
AloI GAACNNNNNNTCC 2 cut(s) 358, 390
Alw21I GWGCWC 4 cut(s) 112, 215, 1000, 1990
Alw26I GTCTC 5 cut(s) 24, 45, 187, 1550, 1964
Alw44I GTGCAC 1 cut(s) 996
AlwI GGATC 5 cut(s) 340, 758, 1926, 2180, 2193
AlwNI CAGNNNCTG 1 cut(s) 1874
Ama87I CYCGRG 1 cut(s) 797
Aor13HI TCCGGA 3 cut(s) 87, 283, 946
AoxI GGCC 9 cut(s) 413, 498, 717, 1389, 1547, 1584, 1822, 2308, 2338
ApaI GGGCCC 1 cut(s) 721
ApaLI GTGCAC 1 cut(s) 996
ApeKI GCWGC 7 cut(s) 82, 102, 1145, 1365, 1397, 1577, 2267
ApoI RAATTY 3 cut(s) 654, 704, 1757
ArsI GACNNNNNNTTYG 2 cut(s) 572, 604
AspLEI GCGC 3 cut(s) 16, 2137, 2214
AspS9I GGNCC 6 cut(s) 286, 337, 413, 717, 718, 1823
AsuC2I CCSGG 2 cut(s) 114, 350
AsuHPI GGTGA 4 cut(s) 217, 1000, 1693, 2139
AvaI CYCGRG 1 cut(s) 797
AvaII GGWCC 2 cut(s) 286, 337
BaeGI GKGCMC 2 cut(s) 721, 1000
BaeI ACNNNNGTAYC 6 cut(s) 555, 588, 722, 755, 1872, 1905
BamHI GGATCC 1 cut(s) 2185
BanI GGYRCC 1 cut(s) 2211
BanII GRGCYC 2 cut(s) 112, 721
BarI GAAGNNNNNNTAC 2 cut(s) 1778, 1810
Bbv12I GWGCWC 4 cut(s) 112, 215, 1000, 1990
BbvCI CCTCAGC 2 cut(s) 1310, 1497
BbvI GCAGC 7 cut(s) 94, 114, 1132, 1377, 1384, 1564, 2279
BccI CCATC 5 cut(s) 196, 981, 1060, 1252, 2324
BceAI ACGGC 2 cut(s) 22, 2312
BciT130I CCWGG 2 cut(s) 643, 1571
BciVI GTATCC 2 cut(s) 649, 1873
BcnI CCSGG 2 cut(s) 114, 350
BcoDI GTCTC 5 cut(s) 24, 45, 187, 1550, 1964
BfaI CTAG 3 cut(s) 2058, 2085, 2120
BfmI CTRYAG 2 cut(s) 1089, 1639
BfoI RGCGCY 1 cut(s) 2215
BfrI CTTAAG 1 cut(s) 536
BfuAI ACCTGC 3 cut(s) 883, 1580, 2140
BfuI GTATCC 2 cut(s) 649, 1873
BglII AGATCT 1 cut(s) 1303
BisI GCNGC 9 cut(s) 83, 103, 1146, 1366, 1398, 1578, 1587, 2268, 2338
BlpI GCTNAGC 2 cut(s) 214, 662
BlsI GCNGC 9 cut(s) 84, 104, 1147, 1367, 1399, 1579, 1588, 2269, 2339
Bme1390I CCNGG 4 cut(s) 114, 350, 643, 1571
Bme18I GGWCC 2 cut(s) 286, 337
BmeT110I CYCGRG 1 cut(s) 797
BmgT120I GGNCC 6 cut(s) 286, 337, 413, 717, 718, 1823
BmiI GGNNCC 5 cut(s) 6, 339, 719, 2187, 2213
BmrFI CCNGG 4 cut(s) 114, 350, 643, 1571
BmsI GCATC 8 cut(s) 505, 635, 981, 988, 1401, 1432, 1990, 2339
BpmI CTGGAG 1 cut(s) 1274
Bpu10I CCTNAGC 3 cut(s) 613, 1310, 1497
Bpu1102I GCTNAGC 2 cut(s) 214, 662
BpuEI CTTGAG 4 cut(s) 755, 1097, 2241, 2268
BpuMI CCSGG 2 cut(s) 114, 350
BsaBI GATNNNNATC 3 cut(s) 885, 1605, 1917
BsaHI GRCGYC 1 cut(s) 2212
BsaI GGTCTC 1 cut(s) 1964
BsaJI CCNNGG 6 cut(s) 349, 642, 1386, 1392, 1569, 1570
BsaWI WCCGGW 4 cut(s) 87, 283, 693, 946
BsaXI ACNNNNNCTCC 2 cut(s) 358, 388
Bsc4I CCNNNNNNNGG 3 cut(s) 112, 700, 727
Bse118I RCCGGY 1 cut(s) 98
Bse1I ACTGG 4 cut(s) 671, 1039, 1587, 2497
Bse3DI GCAATG 2 cut(s) 1164, 2325
Bse8I GATNNNNATC 3 cut(s) 885, 1605, 1917
BseAI TCCGGA 3 cut(s) 87, 283, 946
BseBI CCWGG 2 cut(s) 643, 1571
BseDI CCNNGG 6 cut(s) 349, 642, 1386, 1392, 1569, 1570
BseGI GGATG 8 cut(s) 337, 462, 1416, 1618, 2316, 2330, 2426, 2486
BseJI GATNNNNATC 3 cut(s) 885, 1605, 1917
BseLI CCNNNNNNNGG 3 cut(s) 112, 700, 727
BseMI GCAATG 2 cut(s) 1164, 2325
BseNI ACTGG 4 cut(s) 671, 1039, 1587, 2497
BseRI GAGGAG 2 cut(s) 379, 906
BseSI GKGCMC 2 cut(s) 721, 1000
BseXI GCAGC 7 cut(s) 94, 114, 1132, 1377, 1384, 1564, 2279
BseYI CCCAGC 1 cut(s) 1414
BsgI GTGCAG 4 cut(s) 444, 1206, 1563, 2124
Bsh1236I CGCG 1 cut(s) 2492
BshFI GGCC 9 cut(s) 415, 500, 719, 1391, 1549, 1586, 1824, 2310, 2340
BshNI GGYRCC 1 cut(s) 2211
BsiHKAI GWGCWC 4 cut(s) 112, 215, 1000, 1990
BsiHKCI CYCGRG 1 cut(s) 797
BslFI GGGAC 2 cut(s) 1879, 2130
BslI CCNNNNNNNGG 3 cut(s) 112, 700, 727
BsmAI GTCTC 5 cut(s) 24, 45, 187, 1550, 1964
BsmBI CGTCTC 1 cut(s) 45
BsmFI GGGAC 2 cut(s) 1879, 2130
BsmI GAATGC 2 cut(s) 1228, 1489
BsnI GGCC 9 cut(s) 415, 500, 719, 1391, 1549, 1586, 1824, 2310, 2340
Bso31I GGTCTC 1 cut(s) 1964
BsoBI CYCGRG 1 cut(s) 797
Bsp120I GGGCCC 1 cut(s) 717
Bsp1286I GDGCHC 5 cut(s) 112, 215, 721, 1000, 1990
Bsp13I TCCGGA 3 cut(s) 87, 283, 946
Bsp1407I TGTACA 1 cut(s) 883
Bsp1720I GCTNAGC 2 cut(s) 214, 662
BspANI GGCC 9 cut(s) 415, 500, 719, 1391, 1549, 1586, 1824, 2310, 2340
BspEI TCCGGA 3 cut(s) 87, 283, 946
BspFNI CGCG 1 cut(s) 2492
BspHI TCATGA 2 cut(s) 786, 1618
BspLI GGNNCC 5 cut(s) 6, 339, 719, 2187, 2213
BspMAI CTGCAG 1 cut(s) 1093
BspMI ACCTGC 3 cut(s) 883, 1580, 2140
BspPI GGATC 5 cut(s) 340, 758, 1926, 2180, 2193
BspT107I GGYRCC 1 cut(s) 2211
BspTI CTTAAG 1 cut(s) 536
BspTNI GGTCTC 1 cut(s) 1964
BsrDI GCAATG 2 cut(s) 1164, 2325
BsrFI RCCGGY 1 cut(s) 98
BsrGI TGTACA 1 cut(s) 883
BsrI ACTGG 4 cut(s) 671, 1039, 1587, 2497
BssAI RCCGGY 1 cut(s) 98
BssECI CCNNGG 6 cut(s) 349, 642, 1386, 1392, 1569, 1570
BssNI GRCGYC 1 cut(s) 2212
BssT1I CCWWGG 2 cut(s) 1386, 1392
Bst2UI CCWGG 2 cut(s) 643, 1571
Bst4CI ACNGT 5 cut(s) 1212, 1766, 1885, 1970, 2034
BstACI GRCGYC 1 cut(s) 2212
BstAFI CTTAAG 1 cut(s) 536
BstAPI GCANNNNNTGC 4 cut(s) 547, 824, 1595, 1985
BstAUI TGTACA 1 cut(s) 883
BstC8I GCNNGC 9 cut(s) 48, 100, 357, 668, 816, 996, 1698, 2139, 2522
BstF5I GGATG 8 cut(s) 337, 462, 1416, 1618, 2316, 2330, 2426, 2486
BstFNI CGCG 1 cut(s) 2492
BstH2I RGCGCY 1 cut(s) 2215
BstHHI GCGC 3 cut(s) 16, 2137, 2214
BstMAI GTCTC 5 cut(s) 24, 45, 187, 1550, 1964
BstNI CCWGG 2 cut(s) 643, 1571
BstNSI RCATGY 3 cut(s) 818, 1801, 2524
BstSCI CCNGG 4 cut(s) 112, 348, 641, 1569
BstSFI CTRYAG 2 cut(s) 1089, 1639
BstSLI GKGCMC 2 cut(s) 721, 1000
BstUI CGCG 1 cut(s) 2492
BstV1I GCAGC 7 cut(s) 94, 114, 1132, 1377, 1384, 1564, 2279
BstX2I RGATCY 4 cut(s) 763, 1303, 1918, 2185
BstYI RGATCY 4 cut(s) 763, 1303, 1918, 2185
BsuI GTATCC 2 cut(s) 649, 1873
BsuRI GGCC 9 cut(s) 415, 500, 719, 1391, 1549, 1586, 1824, 2310, 2340
BtsCI GGATG 8 cut(s) 337, 462, 1416, 1618, 2316, 2330, 2426, 2486
BtsI GCAGTG 4 cut(s) 1098, 1530, 1735, 2320
BtsIMutI CAGTG 5 cut(s) 1098, 1530, 1580, 1735, 2320
BveI ACCTGC 3 cut(s) 883, 1580, 2140
Cac8I GCNNGC 9 cut(s) 48, 100, 357, 668, 816, 996, 1698, 2139, 2522
CaiI CAGNNNCTG 1 cut(s) 1874
CciI TCATGA 2 cut(s) 786, 1618
CfoI GCGC 3 cut(s) 16, 2137, 2214
Cfr10I RCCGGY 1 cut(s) 98
Cfr13I GGNCC 6 cut(s) 286, 337, 413, 717, 718, 1823
Csp6I GTAC 6 cut(s) 731, 755, 884, 1042, 1795, 1932
CviAII CATG 8 cut(s) 163, 787, 815, 1439, 1619, 1798, 2521, 2526
CviQI GTAC 6 cut(s) 731, 755, 884, 1042, 1795, 1932
DinI GGCGCC 1 cut(s) 2213
EaeI YGGCCR 2 cut(s) 1584, 2338
EciI GGCGGA 1 cut(s) 2419
Ecl136II GAGCTC 1 cut(s) 110
Eco130I CCWWGG 2 cut(s) 1386, 1392
Eco24I GRGCYC 2 cut(s) 112, 721
Eco31I GGTCTC 1 cut(s) 1964
Eco47I GGWCC 2 cut(s) 286, 337
Eco53kI GAGCTC 1 cut(s) 110
Eco57I CTGAAG 5 cut(s) 500, 651, 794, 999, 1302
Eco88I CYCGRG 1 cut(s) 797
EcoICRI GAGCTC 1 cut(s) 110
EcoO109I RGGNCCY 1 cut(s) 337
EcoRII CCWGG 2 cut(s) 641, 1569
EcoT14I CCWWGG 2 cut(s) 1386, 1392
EcoT22I ATGCAT 1 cut(s) 1440
EcoT38I GRGCYC 2 cut(s) 112, 721
EgeI GGCGCC 1 cut(s) 2213
EheI GGCGCC 1 cut(s) 2213
ErhI CCWWGG 2 cut(s) 1386, 1392
Esp3I CGTCTC 1 cut(s) 45
FaeI CATG 8 cut(s) 166, 790, 818, 1442, 1622, 1801, 2524, 2529
FalI AAGNNNNNCTT 4 cut(s) 840, 872, 2348, 2380
FaqI GGGAC 2 cut(s) 1879, 2130
FatI CATG 8 cut(s) 162, 786, 814, 1438, 1618, 1797, 2520, 2525
FauI CCCGC 3 cut(s) 126, 766, 2029
FauNDI CATATG 2 cut(s) 1996, 2301
Fnu4HI GCNGC 9 cut(s) 83, 103, 1146, 1366, 1398, 1578, 1587, 2268, 2338
FokI GGATG 8 cut(s) 344, 469, 1423, 1625, 2303, 2317, 2413, 2473
FriOI GRGCYC 2 cut(s) 112, 721
Fsp4HI GCNGC 9 cut(s) 83, 103, 1146, 1366, 1398, 1578, 1587, 2268, 2338
FspBI CTAG 3 cut(s) 2058, 2085, 2120
GlaI GCGC 3 cut(s) 15, 2136, 2213
GluI GCNGC 9 cut(s) 83, 103, 1146, 1366, 1398, 1578, 1587, 2268, 2338
GsaI CCCAGC 1 cut(s) 1418
GsuI CTGGAG 1 cut(s) 1274
HaeII RGCGCY 1 cut(s) 2215
HaeIII GGCC 9 cut(s) 415, 500, 719, 1391, 1549, 1586, 1824, 2310, 2340
HhaI GCGC 3 cut(s) 16, 2137, 2214
Hin1I GRCGYC 1 cut(s) 2212
Hin1II CATG 8 cut(s) 166, 790, 818, 1442, 1622, 1801, 2524, 2529
Hin6I GCGC 3 cut(s) 14, 2135, 2212
HinP1I GCGC 3 cut(s) 14, 2135, 2212
HincII GTYRAC 2 cut(s) 1086, 2419
HindII GTYRAC 2 cut(s) 1086, 2419
HindIII AAGCTT 2 cut(s) 1073, 2162
HphI GGTGA 4 cut(s) 217, 1000, 1693, 2139
Hpy166II GTNNAC 6 cut(s) 998, 1086, 1508, 1684, 1803, 2419
Hpy8I GTNNAC 6 cut(s) 998, 1086, 1508, 1684, 1803, 2419
Hpy99I CGWCG 1 cut(s) 1757
HpyAV CCTTC 7 cut(s) 142, 427, 1800, 1936, 2001, 2154, 2371
HpyCH4III ACNGT 5 cut(s) 1212, 1766, 1885, 1970, 2034
HpyCH4IV ACGT 1 cut(s) 2156
HpySE526I ACGT 1 cut(s) 2156
Hsp92I GRCGYC 1 cut(s) 2212
Hsp92II CATG 8 cut(s) 166, 790, 818, 1442, 1622, 1801, 2524, 2529
HspAI GCGC 3 cut(s) 14, 2135, 2212
KasI GGCGCC 1 cut(s) 2211
Kpn2I TCCGGA 3 cut(s) 87, 283, 946
KroI GCCGGC 1 cut(s) 98
KroNI GCCGGC 1 cut(s) 100
Lsp1109I GCAGC 7 cut(s) 94, 114, 1132, 1377, 1384, 1564, 2279
LweI GCATC 8 cut(s) 505, 635, 981, 988, 1401, 1432, 1990, 2339
MaeI CTAG 3 cut(s) 2058, 2085, 2120
MaeII ACGT 1 cut(s) 2156
MaeIII GTNAC 5 cut(s) 248, 385, 1106, 1123, 1273
MfeI CAATTG 4 cut(s) 168, 860, 1227, 2094
MflI RGATCY 4 cut(s) 763, 1303, 1918, 2185
MhlI GDGCHC 5 cut(s) 112, 215, 721, 1000, 1990
Mly113I GGCGCC 1 cut(s) 2212
MlyI GAGTC 5 cut(s) 859, 1184, 1339, 1552, 1908
MmeI TCCRAC 3 cut(s) 551, 2016, 2213
Mph1103I ATGCAT 1 cut(s) 1440
MroI TCCGGA 3 cut(s) 87, 283, 946
MroNI GCCGGC 1 cut(s) 98
MseI TTAA 8 cut(s) 537, 552, 909, 1062, 1542, 1761, 1928, 2166
MslI CAYNNNNRTG 2 cut(s) 819, 1611
MspA1I CMGCKG 1 cut(s) 1496
MspCI CTTAAG 1 cut(s) 536
MspR9I CCNGG 4 cut(s) 114, 350, 643, 1571
MunI CAATTG 4 cut(s) 168, 860, 1227, 2094
Mva1269I GAATGC 2 cut(s) 1228, 1489
MvaI CCWGG 2 cut(s) 643, 1571
MvnI CGCG 1 cut(s) 2492
NaeI GCCGGC 1 cut(s) 100
NarI GGCGCC 1 cut(s) 2212
NciI CCSGG 2 cut(s) 114, 350
NdeI CATATG 2 cut(s) 1996, 2301
NgoMIV GCCGGC 1 cut(s) 98
NlaIII CATG 8 cut(s) 166, 790, 818, 1442, 1622, 1801, 2524, 2529
NlaIV GGNNCC 5 cut(s) 6, 339, 719, 2187, 2213
NmeAIII GCCGAG 1 cut(s) 131
NmuCI GTSAC 2 cut(s) 385, 1123
NsiI ATGCAT 1 cut(s) 1440
NspI RCATGY 3 cut(s) 818, 1801, 2524
PaeI GCATGC 2 cut(s) 818, 2524
PaeR7I CTCGAG 1 cut(s) 797
PagI TCATGA 2 cut(s) 786, 1618
PaqCI CACCTGC 1 cut(s) 2140
PasI CCCWGGG 1 cut(s) 1570
PciI ACATGT 1 cut(s) 1797
PctI GAATGC 2 cut(s) 1228, 1489
PdiI GCCGGC 1 cut(s) 100
PfeI GAWTC 5 cut(s) 91, 125, 790, 1871, 2387
PflFI GACNNNGTC 1 cut(s) 868
PflMI CCANNNNNTGG 1 cut(s) 727
PfoI TCCNGGA 1 cut(s) 112
PkrI GCNGC 9 cut(s) 84, 104, 1147, 1367, 1399, 1579, 1588, 2269, 2339
PleI GAGTC 5 cut(s) 859, 1183, 1338, 1552, 1907
PluTI GGCGCC 1 cut(s) 2215
PpsI GAGTC 5 cut(s) 859, 1183, 1338, 1552, 1907
PpuMI RGGWCCY 1 cut(s) 337
PscI ACATGT 1 cut(s) 1797
PsiI TTATAA 1 cut(s) 1809
Psp124BI GAGCTC 1 cut(s) 112
Psp5II RGGWCCY 1 cut(s) 337
Psp6I CCWGG 2 cut(s) 641, 1569
PspFI CCCAGC 1 cut(s) 1414
PspGI CCWGG 2 cut(s) 641, 1569
PspN4I GGNNCC 5 cut(s) 6, 339, 719, 2187, 2213
PspOMI GGGCCC 1 cut(s) 717
PspPI GGNCC 6 cut(s) 286, 337, 413, 717, 718, 1823
PspPPI RGGWCCY 1 cut(s) 337
PspXI VCTCGAGB 1 cut(s) 797
PstI CTGCAG 1 cut(s) 1093
PstNI CAGNNNCTG 1 cut(s) 1874
PsuI RGATCY 4 cut(s) 763, 1303, 1918, 2185
PsyI GACNNNGTC 1 cut(s) 868
RsaI GTAC 6 cut(s) 732, 756, 885, 1043, 1796, 1933
RsaNI GTAC 6 cut(s) 731, 755, 884, 1042, 1795, 1932
RseI CAYNNNNRTG 2 cut(s) 819, 1611
SacI GAGCTC 1 cut(s) 112
SaqAI TTAA 8 cut(s) 537, 552, 909, 1062, 1542, 1761, 1928, 2166
SatI GCNGC 9 cut(s) 83, 103, 1146, 1366, 1398, 1578, 1587, 2268, 2338
Sau96I GGNCC 6 cut(s) 286, 337, 413, 717, 718, 1823
SchI GAGTC 5 cut(s) 859, 1184, 1339, 1552, 1908
ScrFI CCNGG 4 cut(s) 114, 350, 643, 1571
SduI GDGCHC 5 cut(s) 112, 215, 721, 1000, 1990
SfaNI GCATC 8 cut(s) 505, 635, 981, 988, 1401, 1432, 1990, 2339
SfcI CTRYAG 2 cut(s) 1089, 1639
SfoI GGCGCC 1 cut(s) 2213
Sfr274I CTCGAG 1 cut(s) 797
SinI GGWCC 2 cut(s) 286, 337
SlaI CTCGAG 1 cut(s) 797
SmiMI CAYNNNNRTG 2 cut(s) 819, 1611
SmlI CTYRAG 6 cut(s) 536, 734, 797, 1076, 2256, 2283
SmoI CTYRAG 6 cut(s) 536, 734, 797, 1076, 2256, 2283
SphI GCATGC 2 cut(s) 818, 2524
SspDI GGCGCC 1 cut(s) 2211
SspMI CTAG 3 cut(s) 2058, 2085, 2120
SstI GAGCTC 1 cut(s) 112
StyD4I CCNGG 4 cut(s) 112, 348, 641, 1569
StyI CCWWGG 2 cut(s) 1386, 1392
TaaI ACNGT 5 cut(s) 1212, 1766, 1885, 1970, 2034
TaiI ACGT 1 cut(s) 2159
TaqI TCGA 4 cut(s) 798, 1237, 1752, 1902
TaqII GACCGA 1 cut(s) 573
TatI WGTACW 2 cut(s) 883, 1041
TauI GCSGC 2 cut(s) 1589, 2340
TfiI GAWTC 5 cut(s) 91, 125, 790, 1871, 2387
Tru1I TTAA 8 cut(s) 537, 552, 909, 1062, 1542, 1761, 1928, 2166
Tru9I TTAA 8 cut(s) 537, 552, 909, 1062, 1542, 1761, 1928, 2166
TscAI CASTG 5 cut(s) 1098, 1530, 1587, 1735, 2327
TseFI GTSAC 2 cut(s) 385, 1123
TseI GCWGC 7 cut(s) 82, 102, 1145, 1365, 1397, 1577, 2267
Tsp45I GTSAC 2 cut(s) 385, 1123
TspGWI ACGGA 2 cut(s) 1142, 1172
TspRI CASTG 5 cut(s) 1098, 1530, 1587, 1735, 2327
Tth111I GACNNNGTC 1 cut(s) 868
Van91I CCANNNNNTGG 1 cut(s) 727
Vha464I CTTAAG 1 cut(s) 536
VneI GTGCAC 1 cut(s) 996
VpaK11BI GGWCC 2 cut(s) 286, 337
XapI RAATTY 3 cut(s) 654, 704, 1757
XceI RCATGY 3 cut(s) 818, 1801, 2524
XhoI CTCGAG 1 cut(s) 797
XspI CTAG 3 cut(s) 2058, 2085, 2120
Zsp2I ATGCAT 1 cut(s) 1440
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.