Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0005879

Physical Location & Seq

Genomic Coordinates Forward (+)

12539643 .. 12547230

Gene Structure 7588 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1132700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1950 bp

ATGCCGCTCGGCGAAAGAGCCGGAGACAAGAGCGAGTCTCGGTACTGCGGCGTGGAGACGGAGTTCAACGATGACATGCCCCACGTCCTCGCTTTCAATCTCTCGCATGGATCCTTCGATTTCGTCATTGCTCCTCTGATGGATCCTGCTTATCGACCAAGTTTAATGCAAAAAGACAGTGGTGTATCTGGTGTTCCTCCATTTGCTGGCTCAGACTTGGTTTTGAGTCCTTCCCAATGGAGCAGTCATGTCGTGGGAAAAGTTAGCTCATGGATTGACTTAGATTCAGAAGATGAGATCCTCCGGTGCGATTCAGAAACTACTTTGAAGCAAGAGATAGCATGGGCTTCTCATTTGTCCTTGCAGGCATGCCTTCTTCCAGTTCCAAAGGGTAAATCCTGTGCTAATTATGCTAGATGTGTAAATCAGATTTTACAGGGCCTAAGCAGTATGCAGTTGTGGTTGCGGATTCCATTGGTCAAGTCAGACAGTGAAACCATGGATGTGAACACTGATACCTCGGATGATTCTTGGGAGATTTGGAATTCATTTCGTCTGCTATGTGAACATCACAGTCAGTTATCAGTTGCTCTTGATGTTTTGAGTTCTCTACCTTCTGTAAACTCTCTTGGACGCTGGTTTGGGGAATCTATTAGAGCAGCCATATTCAGTACTGAGTGCTTTCTAACAAATGCCCGAGGCTATCCATGTCTGTCAAAGCGCCACCAAAAGCTTGTTCATGGGTTTTTCAATCATTCTATACAGATAGTTCTATCAGGAGAACTGGTGCATAGTCTTCCCAAGATAAATTCACATATAGCTGCCAATGATAATGATAATAATGTTGATGGTGTCCAGAAACATCCGTTGAGACCGTATTTGGATTATGTTGGCTATCTTTACCAGAGGATGGATCCCCTTCCTGAGCAAGAACGGTTTGAGATTGGTTACAGGGATTACTTGCAGTCACCCTTACAGCCGCTCATGGATAATCTAGAGGCTCAAACCTATGAGACATTTGAGAAGGACACGATGAAATATATCCAGTACCAAAGAGCAATTTGTAAAGCTTTACAGGACAGGGTTCCAGATGACAAGGCATCTTCAATAACTACCGTATTGATGGTTGTAGGAGCAGGACGTGGACCTCTTGTAAGGGCATCATTGCAGGCAGCTGAAGAAACTGGGCGCAAGCTTAAAGTCTACGCTGTTGAAAAAAATCCAAATGCAGTTGTTACACTTCATAGCTTGGTTAAACTGGAGGGGTGGGAAAACATTGTTACCATAATTTCATGTGATATGCGTTACTGGGATGCTCCGGAGAAAGCTGACATTTTGGTTAGTGAATTGTTGGGTTCTTTTGGCGATAACGAGCTGTCTCCTGAGTGCCTTGATGGAGCTCAGAGACTTTTGAAGGATGATGGAATCTCAATACCTTCATCGTACACGAGTTTTATCCAACCGGTGACTGCTTCAAAGCTTTACAATGATGTTAAGTCACATAAAGATATTGCACACTTCGAAACTGCTTATGTTGTTAAATTGCACAACATTGCAAGACTTGCTCCTCCTCAACCTGTGTTCACGTTTGATCATCCAAACAACTCAACTGACAAAAGCAATAACCGGTACACAAAGTTGCAGTTTGGAATAACAAGTGATACTGGCTCAGCCATGATTCACGGATTCGCTGGGTATTTTGATTCAACACTATACAAAGAAGTTCATCTTGGCATTGAACCGTCAACATCAACACCAAACATGTTTAGCTGGTTCCCAATATTTTTCCCTCTAAGGACACCAATTACGCTTAGTCCTGGCGCTTCTCTTGAAGTACATTTTTGGCGGTGTTGTAGTCCTACCAAGGTTTGGTACGAGTGGTGTGTGGCATCACCCTCCAGTTCACCGATTCACAACACCAACGGGCGTTCGTACTGGGTCGGGCTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006325 GO:0006355 GO:0006464 GO:0006479 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008213 GO:0008276 GO:0008469 GO:0008757 GO:0009889 GO:0009909 GO:0009987 GO:0010219 GO:0010220 GO:0010468 GO:0010556 GO:0016043 GO:0016273 GO:0016274 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018193 GO:0018195 GO:0018216 GO:0019219 GO:0019222 GO:0019538 GO:0019918 GO:0031323 GO:0031326 GO:0032259 GO:0034969 GO:0035246 GO:0035247 GO:0036211 GO:0042054 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0043985 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048518 GO:0048580 GO:0048583 GO:0048584 GO:0048831 GO:0050789 GO:0050793 GO:0050794 GO:0051171 GO:0051239 GO:0051252 GO:0051276 GO:0060255 GO:0065007 GO:0071704 GO:0071840 GO:0080090 GO:0080134 GO:0140096 GO:1901564 GO:1903506 GO:2000026 GO:2000112 GO:2000241 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko03013 ko04011 ko04111 map03013 map04011 map04111

Pfam Domains

Protein Families

PRMT5 PRMT5_C PRMT5_TIM

Protein Analysis

650

Amino Acids

72.7

Weight (kDa)

5.55

Isoelectric Point (pI)

44.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PRMT5_TIM	PF17285	39 - 303	1.6e-81	PRMT5 TIM barrel domain
PRMT5	PF05185	309 - 478	1.4e-72	PRMT5 arginine-N-methyltransferase
TRM5-TYW2_MTfase	PF02475	377 - 453	6.8e-06	TRM5/TYW2 methyltransferase domain
PRMT5_C	PF17286	481 - 647	1.2e-62	PRMT5 oligomerisation domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0005879)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G31120 AT4G31120
fragaria_vesca	FvH4_2g30610 FvH4_2g30610 FvH4_2g30610 FvH4_2g30610 FvH4_2g30610
malus_domestica	MD08G1132700.v1.1 MD15G1111800.v1.1 MD15G1112100.v1.1 MD15G1112600.v1.1 MD15G1112700.v1.1
prunus_persica	Prupe.1G463300_v2.0.a1 Prupe.1G463300_v2.0.a1
pyrus_communis	pycom08g11190 pycom15g10130
rosa_chinensis	RchiOBHm_Chr6g0312391
rosa_laevigata	RLG00000010331
rosa_multiflora	Rmu_sc0028838.1_g000001 Rmu_sc0031621.1_g000001 Rmu_ssc0000424.1_g000022
rosa_roxburghii	Rroxscaffold_7G00156840
rosa_rugosa	Rorug06G0395500
rosa_samantha	Rh6AG512700 Rh6BG523600 Rh6CG529000 Rh6DG514900
rosa_wichuraiana	Rw6G044560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	3 cut(s)	206, 910, 1869
AccBSI	CCGCTC	2 cut(s)	7, 982
AccI	GTMKAC	1 cut(s)	1203
AccIII	TCCGGA	1 cut(s)	1318
AciI	CCGC	5 cut(s)	5, 48, 466, 980, 1846
AclWI	GGATC	7 cut(s)	105, 118, 137, 150, 292, 908, 921
AcsI	RAATTY	2 cut(s)	544, 808
AcuI	CTGAAG	1 cut(s)	1197
AfaI	GTAC	8 cut(s)	44, 673, 1049, 1445, 1631, 1836, 1874, 1934
AfiI	CCNNNNNNNGG	3 cut(s)	206, 910, 1869
AflIII	ACRYGT	1 cut(s)	1761
AgeI	ACCGGT	2 cut(s)	1462, 1626
AjiI	CACGTC	2 cut(s)	85, 1142
AjnI	CCWGG	1 cut(s)	1816
AjuI	GAANNNNNNNTTGG	4 cut(s)	720, 752, 1713, 1745
AloI	GAACNNNNNNTCC	2 cut(s)	47, 79
Alw21I	GWGCWC	1 cut(s)	1402
Alw26I	GTCTC	7 cut(s)	18, 42, 50, 865, 1007, 1383, 1399
AlwI	GGATC	7 cut(s)	105, 118, 137, 150, 292, 908, 921
Ama87I	CYCGRG	1 cut(s)	696
Aor13HI	TCCGGA	1 cut(s)	1318
AoxI	GGCC	1 cut(s)	439
ApeKI	GCWGC	3 cut(s)	659, 821, 1172
ApoI	RAATTY	2 cut(s)	544, 808
AsiGI	ACCGGT	2 cut(s)	1462, 1626
AspLEI	GCGC	3 cut(s)	723, 1191, 1823
AspS9I	GGNCC	2 cut(s)	439, 1145
AsuHPI	GGTGA	4 cut(s)	960, 1477, 1884, 1896
AsuII	TTCGAA	1 cut(s)	1521
AvaI	CYCGRG	1 cut(s)	696
AvaII	GGWCC	1 cut(s)	1145
BamHI	GGATCC	3 cut(s)	110, 142, 913
BanII	GRGCYC	2 cut(s)	1402, 1947
BauI	CACGAG	1 cut(s)	1447
BbsI	GAAGAC	1 cut(s)	788
Bbv12I	GWGCWC	1 cut(s)	1402
BbvI	GCAGC	3 cut(s)	671, 808, 1184
BccI	CCATC	6 cut(s)	133, 842, 904, 1117, 1388, 1415
BcgI	CGANNNNNNTGC	2 cut(s)	232, 266
BciT130I	CCWGG	1 cut(s)	1818
BclI	TGATCA	1 cut(s)	1591
BcoDI	GTCTC	7 cut(s)	18, 42, 50, 865, 1007, 1383, 1399
BfaI	CTAG	3 cut(s)	414, 995, 1948
BfoI	RGCGCY	2 cut(s)	724, 1824
BisI	GCNGC	6 cut(s)	5, 49, 660, 822, 980, 1173
BlpI	GCTNAGC	1 cut(s)	1669
BlsI	GCNGC	6 cut(s)	6, 50, 661, 823, 981, 1174
BmcAI	AGTACT	1 cut(s)	673
Bme1390I	CCNGG	1 cut(s)	1818
Bme18I	GGWCC	1 cut(s)	1145
BmeT110I	CYCGRG	1 cut(s)	696
BmgBI	CACGTC	2 cut(s)	85, 1142
BmgT120I	GGNCC	2 cut(s)	439, 1145
BmiI	GGNNCC	5 cut(s)	112, 144, 915, 1086, 1775
BmrFI	CCNGG	1 cut(s)	1818
BmrI	ACTGGG	3 cut(s)	1194, 1318, 1945
BmsI	GCATC	4 cut(s)	1109, 1169, 1303, 1898
BmuI	ACTGGG	3 cut(s)	1194, 1318, 1945
BpiI	GAAGAC	1 cut(s)	788
BplI	GAGNNNNNCTC	2 cut(s)	22, 54
BpmI	CTGGAG	2 cut(s)	1280, 1882
Bpu10I	CCTNAGC	2 cut(s)	443, 924
Bpu1102I	GCTNAGC	1 cut(s)	1669
Bpu14I	TTCGAA	1 cut(s)	1521
BsaI	GGTCTC	1 cut(s)	865
BsaJI	CCNNGG	4 cut(s)	498, 519, 697, 1863
BsaWI	WCCGGW	4 cut(s)	303, 1318, 1462, 1626
BsaXI	ACNNNNNCTCC	4 cut(s)	47, 77, 771, 801
Bsc4I	CCNNNNNNNGG	3 cut(s)	206, 910, 1869
Bse118I	RCCGGY	2 cut(s)	1462, 1626
Bse1I	ACTGG	9 cut(s)	380, 789, 1045, 1189, 1263, 1313, 1669, 1899, 1940
Bse3DI	GCAATG	3 cut(s)	126, 1163, 1551
BseAI	TCCGGA	1 cut(s)	1318
BseBI	CCWGG	1 cut(s)	1818
BseDI	CCNNGG	4 cut(s)	498, 519, 697, 1863
BseGI	GGATG	7 cut(s)	508, 529, 862, 915, 1318, 1423, 1594
BseLI	CCNNNNNNNGG	3 cut(s)	206, 910, 1869
BseMI	GCAATG	3 cut(s)	126, 1163, 1551
BseMII	CTCAG	6 cut(s)	225, 666, 915, 1374, 1415, 1683
BseNI	ACTGG	9 cut(s)	380, 789, 1045, 1189, 1263, 1313, 1669, 1899, 1940
BseRI	GAGGAG	3 cut(s)	123, 1557, 1560
BseXI	GCAGC	3 cut(s)	671, 808, 1184
BseYI	CCCAGC	1 cut(s)	1691
BshFI	GGCC	1 cut(s)	441
BshTI	ACCGGT	2 cut(s)	1462, 1626
BsiHKAI	GWGCWC	1 cut(s)	1402
BsiHKCI	CYCGRG	1 cut(s)	696
BsiSI	CCGG	5 cut(s)	21, 304, 1319, 1463, 1627
BslI	CCNNNNNNNGG	3 cut(s)	206, 910, 1869
BsmAI	GTCTC	7 cut(s)	18, 42, 50, 865, 1007, 1383, 1399
BsmBI	CGTCTC	1 cut(s)	50
BsnI	GGCC	1 cut(s)	441
Bso31I	GGTCTC	1 cut(s)	865
BsoBI	CYCGRG	1 cut(s)	696
Bsp119I	TTCGAA	1 cut(s)	1521
Bsp1286I	GDGCHC	2 cut(s)	1402, 1947
Bsp13I	TCCGGA	1 cut(s)	1318
Bsp143I	GATC	5 cut(s)	110, 142, 297, 913, 1591
Bsp1720I	GCTNAGC	1 cut(s)	1669
Bsp19I	CCATGG	1 cut(s)	498
BspACI	CCGC	5 cut(s)	5, 48, 466, 980, 1846
BspANI	GGCC	1 cut(s)	441
BspCNI	CTCAG	6 cut(s)	224, 667, 916, 1375, 1414, 1682
BspEI	TCCGGA	1 cut(s)	1318
BspLI	GGNNCC	5 cut(s)	112, 144, 915, 1086, 1775
BspPI	GGATC	7 cut(s)	105, 118, 137, 150, 292, 908, 921
BspT104I	TTCGAA	1 cut(s)	1521
BspTNI	GGTCTC	1 cut(s)	865
BsrBI	CCGCTC	2 cut(s)	7, 982
BsrDI	GCAATG	3 cut(s)	126, 1163, 1551
BsrFI	RCCGGY	2 cut(s)	1462, 1626
BsrI	ACTGG	9 cut(s)	380, 789, 1045, 1189, 1263, 1313, 1669, 1899, 1940
BssAI	RCCGGY	2 cut(s)	1462, 1626
BssECI	CCNNGG	4 cut(s)	498, 519, 697, 1863
BssMI	GATC	5 cut(s)	110, 142, 297, 913, 1591
BssSI	CACGAG	1 cut(s)	1447
BssT1I	CCWWGG	2 cut(s)	498, 1863
Bst2BI	CACGAG	1 cut(s)	1447
Bst2UI	CCWGG	1 cut(s)	1818
Bst4CI	ACNGT	7 cut(s)	179, 491, 575, 876, 936, 1117, 1743
BstAPI	GCANNNNNTGC	1 cut(s)	1562
BstBI	TTCGAA	1 cut(s)	1521
BstC8I	GCNNGC	5 cut(s)	208, 366, 370, 1170, 1193
BstDSI	CCRYGG	1 cut(s)	498
BstF5I	GGATG	7 cut(s)	508, 529, 862, 915, 1318, 1423, 1594
BstH2I	RGCGCY	2 cut(s)	724, 1824
BstHHI	GCGC	3 cut(s)	723, 1191, 1823
BstKTI	GATC	5 cut(s)	113, 145, 300, 916, 1594
BstMAI	GTCTC	7 cut(s)	18, 42, 50, 865, 1007, 1383, 1399
BstMBI	GATC	5 cut(s)	110, 142, 297, 913, 1591
BstMWI	GCNNNNNNNGC	2 cut(s)	410, 1562
BstNI	CCWGG	1 cut(s)	1818
BstNSI	RCATGY	3 cut(s)	79, 372, 1765
BstSCI	CCNGG	1 cut(s)	1816
BstV1I	GCAGC	3 cut(s)	671, 808, 1184
BstV2I	GAAGAC	1 cut(s)	788
BstX2I	RGATCY	4 cut(s)	110, 142, 297, 913
BstYI	RGATCY	4 cut(s)	110, 142, 297, 913
BsuRI	GGCC	1 cut(s)	441
BtgI	CCRYGG	1 cut(s)	498
BtrI	CACGTC	2 cut(s)	85, 1142
BtsCI	GGATG	7 cut(s)	508, 529, 862, 915, 1318, 1423, 1594
BtsIMutI	CAGTG	3 cut(s)	184, 496, 510
Cac8I	GCNNGC	5 cut(s)	208, 366, 370, 1170, 1193
CfoI	GCGC	3 cut(s)	723, 1191, 1823
Cfr10I	RCCGGY	2 cut(s)	1462, 1626
Cfr13I	GGNCC	2 cut(s)	439, 1145
CseI	GACGC	1 cut(s)	642
Csp6I	GTAC	8 cut(s)	43, 672, 1048, 1444, 1630, 1835, 1873, 1933
CspAI	ACCGGT	2 cut(s)	1462, 1626
CviQI	GTAC	8 cut(s)	43, 672, 1048, 1444, 1630, 1835, 1873, 1933
DpnI	GATC	5 cut(s)	112, 144, 299, 915, 1593
DpnII	GATC	5 cut(s)	110, 142, 297, 913, 1591
Ecl136II	GAGCTC	1 cut(s)	1400
Eco130I	CCWWGG	2 cut(s)	498, 1863
Eco24I	GRGCYC	2 cut(s)	1402, 1947
Eco31I	GGTCTC	1 cut(s)	865
Eco47I	GGWCC	1 cut(s)	1145
Eco53kI	GAGCTC	1 cut(s)	1400
Eco57I	CTGAAG	1 cut(s)	1197
Eco88I	CYCGRG	1 cut(s)	696
EcoICRI	GAGCTC	1 cut(s)	1400
EcoO109I	RGGNCCY	1 cut(s)	439
EcoRI	GAATTC	1 cut(s)	544
EcoRII	CCWGG	1 cut(s)	1816
EcoT14I	CCWWGG	2 cut(s)	498, 1863
EcoT38I	GRGCYC	2 cut(s)	1402, 1947
ErhI	CCWWGG	2 cut(s)	498, 1863
Esp3I	CGTCTC	1 cut(s)	50
FalI	AAGNNNNNCTT	2 cut(s)	1713, 1745
FbaI	TGATCA	1 cut(s)	1591
FblI	GTMKAC	1 cut(s)	1203
Fnu4HI	GCNGC	6 cut(s)	5, 49, 660, 822, 980, 1173
FokI	GGATG	7 cut(s)	515, 536, 849, 922, 1325, 1430, 1581
FriOI	GRGCYC	2 cut(s)	1402, 1947
Fsp4HI	GCNGC	6 cut(s)	5, 49, 660, 822, 980, 1173
FspBI	CTAG	3 cut(s)	414, 995, 1948
GlaI	GCGC	3 cut(s)	722, 1190, 1822
GluI	GCNGC	6 cut(s)	5, 49, 660, 822, 980, 1173
GsaI	CCCAGC	1 cut(s)	1695
GsuI	CTGGAG	2 cut(s)	1280, 1882
HaeII	RGCGCY	2 cut(s)	724, 1824
HaeIII	GGCC	1 cut(s)	441
HapII	CCGG	5 cut(s)	21, 304, 1319, 1463, 1627
HgaI	GACGC	1 cut(s)	642
HhaI	GCGC	3 cut(s)	723, 1191, 1823
Hin6I	GCGC	3 cut(s)	721, 1189, 1821
HinP1I	GCGC	3 cut(s)	721, 1189, 1821
HincII	GTYRAC	1 cut(s)	1746
HindII	GTYRAC	1 cut(s)	1746
HindIII	AAGCTT	4 cut(s)	731, 1068, 1193, 1478
HpaII	CCGG	5 cut(s)	21, 304, 1319, 1463, 1627
HphI	GGTGA	4 cut(s)	960, 1477, 1884, 1896
Hpy188I	TCNGA	8 cut(s)	138, 214, 289, 316, 429, 487, 523, 1404
Hpy188III	TCNNGA	9 cut(s)	593, 777, 856, 923, 995, 1088, 1319, 1382, 1829
HpyAV	CCTTC	8 cut(s)	124, 240, 383, 624, 929, 1018, 1408, 1446
HpyCH4III	ACNGT	7 cut(s)	179, 491, 575, 876, 936, 1117, 1743
HpyCH4IV	ACGT	3 cut(s)	84, 1141, 1586
HpyF10VI	GCNNNNNNNGC	2 cut(s)	410, 1562
HpySE526I	ACGT	3 cut(s)	84, 1141, 1586
HspAI	GCGC	3 cut(s)	721, 1189, 1821
Kpn2I	TCCGGA	1 cut(s)	1318
Ksp22I	TGATCA	1 cut(s)	1591
Kzo9I	GATC	5 cut(s)	110, 142, 297, 913, 1591
LmnI	GCTCC	6 cut(s)	136, 240, 1133, 1321, 1397, 1570
Lsp1109I	GCAGC	3 cut(s)	671, 808, 1184
LweI	GCATC	4 cut(s)	1109, 1169, 1303, 1898
MaeI	CTAG	3 cut(s)	414, 995, 1948
MaeII	ACGT	3 cut(s)	84, 1141, 1586
MaeIII	GTNAC	7 cut(s)	947, 966, 1234, 1279, 1304, 1465, 1497
MalI	GATC	5 cut(s)	112, 144, 299, 915, 1593
MbiI	CCGCTC	2 cut(s)	7, 982
MboI	GATC	5 cut(s)	110, 142, 297, 913, 1591
MboII	GAAGA	5 cut(s)	302, 368, 788, 1095, 1190
MflI	RGATCY	4 cut(s)	110, 142, 297, 913
MhlI	GDGCHC	2 cut(s)	1402, 1947
MluCI	AATT	8 cut(s)	406, 544, 808, 1059, 1287, 1346, 1541, 1803
MlyI	GAGTC	2 cut(s)	44, 235
MmeI	TCCRAC	1 cut(s)	1483
MroI	TCCGGA	1 cut(s)	1318
MseI	TTAA	5 cut(s)	164, 1197, 1254, 1494, 1539
MslI	CAYNNNNRTG	1 cut(s)	503
MspA1I	CMGCKG	1 cut(s)	1175
MspI	CCGG	5 cut(s)	21, 304, 1319, 1463, 1627
MspR9I	CCNGG	1 cut(s)	1818
MvaI	CCWGG	1 cut(s)	1818
MwoI	GCNNNNNNNGC	2 cut(s)	410, 1562
NcoI	CCATGG	1 cut(s)	498
NdeII	GATC	5 cut(s)	110, 142, 297, 913, 1591
NlaIV	GGNNCC	5 cut(s)	112, 144, 915, 1086, 1775
NmuCI	GTSAC	3 cut(s)	966, 1465, 1497
NspI	RCATGY	3 cut(s)	79, 372, 1765
NspV	TTCGAA	1 cut(s)	1521
PaeI	GCATGC	1 cut(s)	372
PciI	ACATGT	1 cut(s)	1761
PflMI	CCANNNNNTGG	3 cut(s)	206, 910, 1869
PinAI	ACCGGT	2 cut(s)	1462, 1626
PkrI	GCNGC	6 cut(s)	6, 50, 661, 823, 981, 1174
PleI	GAGTC	2 cut(s)	43, 234
PpsI	GAGTC	2 cut(s)	43, 234
PscI	ACATGT	1 cut(s)	1761
Psp124BI	GAGCTC	1 cut(s)	1402
Psp6I	CCWGG	1 cut(s)	1816
PspFI	CCCAGC	1 cut(s)	1691
PspGI	CCWGG	1 cut(s)	1816
PspN4I	GGNNCC	5 cut(s)	112, 144, 915, 1086, 1775
PspPI	GGNCC	2 cut(s)	439, 1145
PsrI	GAACNNNNNNTAC	2 cut(s)	177, 209
PsuI	RGATCY	4 cut(s)	110, 142, 297, 913
PvuII	CAGCTG	1 cut(s)	1175
RsaI	GTAC	8 cut(s)	44, 673, 1049, 1445, 1631, 1836, 1874, 1934
RsaNI	GTAC	8 cut(s)	43, 672, 1048, 1444, 1630, 1835, 1873, 1933
RseI	CAYNNNNRTG	1 cut(s)	503
SacI	GAGCTC	1 cut(s)	1402
SaqAI	TTAA	5 cut(s)	164, 1197, 1254, 1494, 1539
SatI	GCNGC	6 cut(s)	5, 49, 660, 822, 980, 1173
Sau3AI	GATC	5 cut(s)	110, 142, 297, 913, 1591
Sau96I	GGNCC	2 cut(s)	439, 1145
ScaI	AGTACT	1 cut(s)	673
SchI	GAGTC	2 cut(s)	44, 235
ScrFI	CCNGG	1 cut(s)	1818
SduI	GDGCHC	2 cut(s)	1402, 1947
SfaNI	GCATC	4 cut(s)	1109, 1169, 1303, 1898
SfuI	TTCGAA	1 cut(s)	1521
SinI	GGWCC	1 cut(s)	1145
SmiMI	CAYNNNNRTG	1 cut(s)	503
SphI	GCATGC	1 cut(s)	372
Sse9I	AATT	8 cut(s)	406, 544, 808, 1059, 1287, 1346, 1541, 1803
SsiI	CCGC	5 cut(s)	5, 48, 466, 980, 1846
SspI	AATATT	1 cut(s)	1782
SspMI	CTAG	3 cut(s)	414, 995, 1948
SstI	GAGCTC	1 cut(s)	1402
StyD4I	CCNGG	1 cut(s)	1816
StyI	CCWWGG	2 cut(s)	498, 1863
TaaI	ACNGT	7 cut(s)	179, 491, 575, 876, 936, 1117, 1743
TaiI	ACGT	3 cut(s)	87, 1144, 1589
TaqI	TCGA	3 cut(s)	117, 154, 1521
TasI	AATT	8 cut(s)	406, 544, 808, 1059, 1287, 1346, 1541, 1803
TatI	WGTACW	2 cut(s)	671, 1834
TauI	GCSGC	3 cut(s)	7, 51, 982
Tru1I	TTAA	5 cut(s)	164, 1197, 1254, 1494, 1539
Tru9I	TTAA	5 cut(s)	164, 1197, 1254, 1494, 1539
TscAI	CASTG	3 cut(s)	184, 496, 517
TseFI	GTSAC	3 cut(s)	966, 1465, 1497
TseI	GCWGC	3 cut(s)	659, 821, 1172
Tsp45I	GTSAC	3 cut(s)	966, 1465, 1497
TspDTI	ATGAA	7 cut(s)	537, 728, 1049, 1232, 1281, 1428, 1715
TspGWI	ACGGA	3 cut(s)	74, 855, 1698
TspRI	CASTG	3 cut(s)	184, 496, 517
Van91I	CCANNNNNTGG	3 cut(s)	206, 910, 1869
VpaK11BI	GGWCC	1 cut(s)	1145
XapI	RAATTY	2 cut(s)	544, 808
XbaI	TCTAGA	1 cut(s)	994
XceI	RCATGY	3 cut(s)	79, 372, 1765
XmiI	GTMKAC	1 cut(s)	1203
XspI	CTAG	3 cut(s)	414, 995, 1948
ZrmI	AGTACT	1 cut(s)	673

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.