Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0005879

Physical Location & Seq

Genomic Coordinates Forward (+)

735189 .. 747039

Gene Structure 11851 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000010331

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4068 bp

ATGCCGCTTGGGGAAAGAGCAGGTGACAAGAGCGAGTCTCGCTACTGCGGCGTGGAGACGGAGTTCAGCGATGACATGCCCAACGTCCTCGCCTTCAATCTCACCCATGGCTCCTTCGACTTCGTCGTCGCTACTCTGATGGATCCTGCTTATCGGCCAAGCTTAATGCAAAAGAACGGTGGTGCATCTAGTGTTCTTCCATTTGCTGGGTCAGACTTGGTTTTGAGCCCTGCCCAATGGAGCAGTCATGTCGTGGGAAAAATTAGCTCCTGGATTGACTTGGATTCGGAAGACGAGATCCTCCGAAGCGATTCAGAAATTACCTTGAAGCAGGAGATAGCATGGGCGTCTCATTTGTCCCTGCAGGCATGTCTTCTTCCCTCTCCAAAGGGAAAGACCTGTGCTAACTATGCTAGGTGTGTAAATCAGATTTTACAGGGTCTACAAAGTATGCAGTTGTGGCTTCGGATTCCTTTGGTCAAGACTGATAATTACACGGTTTCTGTTAACTCTGATGATTTGGATGATTCTTGGGAGACTTGGAATTCATTTCGTCTGCTGTGTGAACATCACAGTCAATTATCAGTTGTCCTTGATGTTTCGAGTTCACTACCTTCTGCAAATTCACTTGGACGTTGGTTTGGAGAGTCCGTCCGAGCAGCCATATTCTGTACTGATTCCTTTCTAACAAATCCACGAGGTTATCCATGCCTGTCAAAGCGTCACCAAAAGCTAGTGTCTGGGTTTTTCAATCATTCTATACAGATAGTTCTATCAGGACAATCTGTACACAGTCTTACCAAGATGAATTCAGACATAGCTGCAAATAATAATGAAAACAATGTTGATGGTGTACAGAAACATCCCTTGAGACCTTATTTGGATTACGTTGGCTTTCTTTACCAAAGGATGGATCCTCTTCCTGAGCAAGAACGGTTTGAGCTTGGTTACAGGGATTTCTTGCAGTCACCTTTACAGCCACTCATGGATAATCTAGAGGCTCAGACTTATGAGACATTTGAAAAGGATACAACAAAATACATTCAGTACCAAAGGGCAATTGCTAAAGCTTTACAGGACAGGGTTGCAGATGAAAAGTCTTCTACAGTAACCACTGTATTAATGGTTGTTGGAGCAGGGCGTGGACCACTAGTTAGGGCGTCACTGCAGGCAGCTGAAGAAACTGGGCGCAGGATTAAAGTCTACGCTGTGGAAAAAAATCCAAATGCAGTAGTTACACTTCATAGCTTGGTTAAACTAGAGGGCTGGGAAAACATTGTTTCCATAATTTCAAGCGATATGCGTCATTGGGATGCTCCTGAGAAAGCTGACATTTTGGTTAGTGAATTATTGGGTTCTTTTGGTGATAATGAGCTGTCTCCTGAGTGTCTTGATGGAGCCCAGAAATTTCTGAAAGAAGATGGAATCTCAATACCTTCATCGTATACGAGTTTCATCCATCCGGTGACTGCTTCAAAACTTTACAATGATGTTAAGGCGCATAAAGATGTTGCACACTTTGAAACTGCTTATGTTGTTAAATTGCACAACATTGCAAGACTGGCTCCTCCTCAACCTGTGTTCACGTTCACTCATCCAAACCGATCAATTGACAAAAGCAATAGCCGCTACACGAAGTTAAAGTTTGAAATACCTGGTGATACTGGGTCTGCTATGGTTCATGGATTTGCTGGCTACTTTGATTCCATACTGTACAAAGAAGTTCATCTTGGCATTGAGCCATCAACATCAACACCAAACATGTTTAGCTGGTTTCCCATATTTTTTCCATTAAGGACACCAATTTGTCTGCGTCCTGGTTCTTCTCTCGAAGTGCATTTTTGGCGTTGTTGTAGTCCTACCAAGGTCTGGTACGAATGGGGTGTGGCATCACCCAGCAGTTCATCCATTCACAACAGCAATGATCTTTTCCTCTTCCGCCTCCGGCAATCCATAGGTGATTATAATATGTACAACGGAATAGGGTTACAGACTCCGAGAGGGTCCGGAACCAATGGCTATATCCAGACGAACAAGTTCTTCATCAAATCCAAGACCGGCAAGGTCGCCGCCGAGAACTCCAGAGGCTTCGAGGGCGACCAGGGCATGGCCGGCGTCTCCAAGAAGCCCAACAGAGACATCCTTGAGCACGATCGCAAGCGCCAGATTGAACTCAAGCTTGTCGTCCTCGAGGACAAGCTAACCGATCAGGGTTTCACCGACGCTGAGATTGCTGACAAGCTTGCCCAGGCCAGGAGGACTCTCGAAGCCGCCGCCGCCTCCGAGGACATGGGTGGACCCACTGCCTTTGTTCTTGGAGATAACAAGGTTTCAGATACGCAGACCCACCAAATTGCTGCTAGGAAGCAGAAGCAGATGGACAACTTAAGAGATGCGTTGAAACTAAAACCTGAAGCTACAGAAACAAATACTGAGGAGATCGAGGATGGACTAATCATTAGTCCGAAGAATGATGATAGCAGGCATTATGAGAGGAAAGAGCATTCGTTTTTGGACAGAGAATCTGGGTGGAAGAAATCTGTGGAGGATAAGCACAAGGTCGAGAAAGATAAGAAAAAGAGCGTTAAAGAATCTAGGAGGAACAAAAAGAAAGAAAGTAGGAAGAGAAGGCATAAGGATGATTCTTCTGATACAGATAGCAGTGGTAGCCCTCTGAAACCTGATAAAAAGAAAAAGCATCGGAGTAGAAGTAGCAGCAGTGAAGAAGATGATTCTGAAGTTGATACATCGAAAAAGGAAGTTGAGAAATACAAAAAACGGGAGAAGAGGTACGATTCAGAAGAAGATGATTCTGAAGTTGATGTTGACGAGAAGCGGCAGGTTGTGAAGAAGCATAGTAAAAGCCGAAACAATAACACTGATGATTCTGCTTCCAGTGAAGATGCCGAGGATAGATCCACAAAGGAAGTCAAGAAATCCAAACAACCTCATAGGAGGCATGATTCAGATGAAGATGGTTCTGAAGATGATGTTGACAAGAAGAGGAAGGTTGTAAAGAAGCATAGTAAAAGCAGAAACGATGATACTGATGATTCTGCTGCCAGTGAAGATGTCGAGGATAGATCCACAAAGGAAGTCAAGAAATCCAAACAACCTCGTAGGAGGCATGATTCAGATGAAGATGGTTCTGAAGATGATGTTGACAAGAAGAGGAAGGTTGTAAAGAAGCATAGTAAAAGCAGAAACGATGATACTGATGATTCTGCTGCCAGTGAAGATGCCGAGGATAGATCCACAAAGGAAGTCAGGAAATTCCAACAACCTCGTAGGAGGCATGATTCAGATGATGATTCTGGTTATCCTGAAGAACTGCCAAAGAGCAGAACTGAAAAGGGCAAACAACATGTGAAACCAAACAAAAGGCATGACTCTGGGGATGAGTCTGATTTTGACAGCAGAATGGAAAGGAAAAGTAGCCAAGTTGAGCAGCAAAGGAATCAGCAGGTCAGCCGCAGGGTAGATCGAGACGATTTTGATGTGGAGAAAGCCGACAATGGAAAAAGCAGAAAATTGTATGATAGTGATTCTGACTATGAAAGATCCAGAAAAAGTAGAGGTGCAATGACAGAAAAGAGTAGAAGAAGTGGCAGGAATGATACTAGTGATGATGATACTAATATTGGGAGGAAACCTGGAAGGGTTGAAGAGGGTGACGGAGGACGTGGAAGGCACAACAAGGGTGAAATGCAAAGAGGAAGGAAGAATGAAAGAGATGAAGAGGATCATGATTATAGAAGGCCGAGGGAAGGTCTATTACAGGAGCTTGGAAATAGAAAGCGTGATCAAGTGAGAGGAGAAGGCATGAAACTGACAGACATAATGAAGAGGAGCATAAGTCTAGAAGGCATGGAGAAGATGAAGGGGAGCGTAGAAACAGAAGACATGGAAAACTGGAGGAACAACAGGGAAATAAGGAGATTGAGAGAGATAGACAGGATTCCAGATCAAGTGAGAGAAGAAGGTATGAGAATGCCAAGCACAATGACGGTCGGTCCAGGCATCGTGACTGATTCTATGTTTACATGGCCTCAAAGCTTTTGTAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006325 GO:0006355 GO:0006464 GO:0006479 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008213 GO:0008276 GO:0008469 GO:0008757 GO:0009889 GO:0009909 GO:0009987 GO:0010219 GO:0010220 GO:0010468 GO:0010556 GO:0016043 GO:0016273 GO:0016274 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018193 GO:0018195 GO:0018216 GO:0019219 GO:0019222 GO:0019538 GO:0019918 GO:0031323 GO:0031326 GO:0032259 GO:0034969 GO:0035246 GO:0035247 GO:0036211 GO:0042054 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0043985 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048518 GO:0048580 GO:0048583 GO:0048584 GO:0048831 GO:0050789 GO:0050793 GO:0050794 GO:0051171 GO:0051239 GO:0051252 GO:0051276 GO:0060255 GO:0065007 GO:0071704 GO:0071840 GO:0080090 GO:0080134 GO:0140096 GO:1901564 GO:1903506 GO:2000026 GO:2000112 GO:2000241 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko03013 ko04011 ko04111 map03013 map04011 map04111

Pfam Domains

Protein Families

PRMT5 PRMT5_C PRMT5_TIM

Protein Analysis

1356

Amino Acids

154.11

Weight (kDa)

6.49

Isoelectric Point (pI)

54.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PRMT5_TIM	PF17285	39 - 302	5.8e-78	PRMT5 TIM barrel domain
PRMT5	PF05185	309 - 478	2.6e-73	PRMT5 arginine-N-methyltransferase
TRM5-TYW2_MTfase	PF02475	361 - 453	1.8e-06	TRM5/TYW2 methyltransferase domain
PRMT5_C	PF17286	481 - 641	1.2e-58	PRMT5 oligomerisation domain
cwf21	PF08312	715 - 757	3.2e-14	cwf21 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005879)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G31120 AT4G31120
fragaria_vesca	FvH4_2g30610 FvH4_2g30610 FvH4_2g30610 FvH4_2g30610 FvH4_2g30610
malus_domestica	MD08G1132700.v1.1 MD15G1111800.v1.1 MD15G1112100.v1.1 MD15G1112600.v1.1 MD15G1112700.v1.1
prunus_persica	Prupe.1G463300_v2.0.a1 Prupe.1G463300_v2.0.a1
pyrus_communis	pycom08g11190 pycom15g10130
rosa_chinensis	RchiOBHm_Chr6g0312391
rosa_laevigata	RLG00000010331
rosa_multiflora	Rmu_sc0028838.1_g000001 Rmu_sc0031621.1_g000001 Rmu_ssc0000424.1_g000022
rosa_roxburghii	Rroxscaffold_7G00156840
rosa_rugosa	Rorug06G0395500
rosa_samantha	Rh6AG512700 Rh6BG523600 Rh6CG529000 Rh6DG514900
rosa_wichuraiana	Rw6G044560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1965
AarI	CACCTGC	1 cut(s)	11
AasI	GACNNNNNNGTC	1 cut(s)	125
AbsI	CCTCGAGG	1 cut(s)	2189
Acc36I	ACCTGC	3 cut(s)	11, 2830, 3454
AccB7I	CCANNNNNTGG	4 cut(s)	206, 910, 1869, 2107
AccI	GTMKAC	3 cut(s)	442, 1203, 1445
AccIII	TCCGGA	1 cut(s)	2006
AcoI	YGGCCR	2 cut(s)	155, 2109
AcsI	RAATTY	5 cut(s)	544, 622, 808, 1406, 3272
AcuI	CTGAAG	7 cut(s)	1197, 2433, 2757, 2835, 3003, 3171, 3345
AcyI	GRCGYC	3 cut(s)	347, 1160, 2115
AfaI	GTAC	8 cut(s)	673, 789, 855, 1049, 1715, 1874, 1973, 2792
AfiI	CCNNNNNNNGG	6 cut(s)	206, 910, 1869, 2107, 2253, 3767
AflII	CTTAAG	1 cut(s)	2386
AflIII	ACRYGT	2 cut(s)	1761, 3364
AhdI	GACNNNNNGTC	1 cut(s)	4011
AhlI	ACTAGT	2 cut(s)	1150, 3620
AjiI	CACGTC	1 cut(s)	3683
AjnI	CCWGG	8 cut(s)	269, 1654, 1816, 2100, 2247, 2252, 3652, 4015
AjuI	GAANNNNNNNTTGG	2 cut(s)	1713, 1745
AloI	GAACNNNNNNTCC	2 cut(s)	47, 79
Alw21I	GWGCWC	1 cut(s)	2151
Ama87I	CYCGRG	1 cut(s)	2189
Aor13HI	TCCGGA	1 cut(s)	2006
AoxI	GGCC	5 cut(s)	155, 2109, 2250, 3758, 4046
ApeKI	GCWGC	8 cut(s)	659, 821, 1172, 2357, 2715, 3059, 3227, 3448
ApoI	RAATTY	5 cut(s)	544, 622, 808, 1406, 3272
AseI	ATTAAT	1 cut(s)	1121
Asp700I	GAANNNNTTC	2 cut(s)	310, 2036
AspLEI	GCGC	3 cut(s)	1191, 1501, 2163
AspS9I	GGNCC	4 cut(s)	1145, 2004, 2297, 4013
AvaI	CYCGRG	1 cut(s)	2189
AvaII	GGWCC	4 cut(s)	1145, 2004, 2297, 4013
BamHI	GGATCC	2 cut(s)	142, 913
BanII	GRGCYC	2 cut(s)	230, 1402
BarI	GAAGNNNNNNTAC	4 cut(s)	1224, 1256, 3759, 3791
BauI	CACGAG	1 cut(s)	696
BbsI	GAAGAC	4 cut(s)	297, 365, 1092, 3906
Bbv12I	GWGCWC	1 cut(s)	2151
BbvI	GCAGC	8 cut(s)	671, 808, 1184, 2344, 2727, 3046, 3214, 3460
BcgI	CGANNNNNNTGC	2 cut(s)	232, 266
BciT130I	CCWGG	8 cut(s)	271, 1656, 1818, 2102, 2249, 2254, 3654, 4017
BciVI	GTATCC	1 cut(s)	1021
BclI	TGATCA	1 cut(s)	3802
BcuI	ACTAGT	2 cut(s)	1150, 3620
BfmI	CTRYAG	4 cut(s)	362, 1104, 1166, 2418
BfoI	RGCGCY	1 cut(s)	2164
BfrI	CTTAAG	1 cut(s)	2386
BfuAI	ACCTGC	3 cut(s)	11, 2830, 3454
BfuI	GTATCC	1 cut(s)	1021
Bme1390I	CCNGG	8 cut(s)	271, 1656, 1818, 2102, 2249, 2254, 3654, 4017
Bme18I	GGWCC	4 cut(s)	1145, 2004, 2297, 4013
BmeRI	GACNNNNNGTC	1 cut(s)	4011
BmeT110I	CYCGRG	1 cut(s)	2189
BmgBI	CACGTC	1 cut(s)	3683
BmgT120I	GGNCC	4 cut(s)	1145, 2004, 2297, 4013
BmiI	GGNNCC	8 cut(s)	112, 144, 915, 1399, 1566, 2005, 2011, 2299
BmrFI	CCNGG	8 cut(s)	271, 1656, 1818, 2102, 2249, 2254, 3654, 4017
BmrI	ACTGGG	2 cut(s)	1194, 1674
BmsI	GCATC	8 cut(s)	194, 1303, 1898, 2383, 2707, 2893, 3229, 4029
BmuI	ACTGGG	2 cut(s)	1194, 1674
BpiI	GAAGAC	4 cut(s)	297, 365, 1092, 3906
BplI	GAGNNNNNCTC	2 cut(s)	22, 54
BpmI	CTGGAG	2 cut(s)	2065, 3934
Bpu10I	CCTNAGC	1 cut(s)	924
BpuEI	CTTGAG	3 cut(s)	889, 2159, 2165
BsaHI	GRCGYC	3 cut(s)	347, 1160, 2115
BsaI	GGTCTC	1 cut(s)	865
BsaJI	CCNNGG	8 cut(s)	106, 1863, 2101, 2247, 2283, 2907, 3243, 3761
BsaWI	WCCGGW	2 cut(s)	1462, 2006
Bsc4I	CCNNNNNNNGG	6 cut(s)	206, 910, 1869, 2107, 2253, 3767
Bse118I	RCCGGY	2 cut(s)	2057, 2111
Bse1I	ACTGG	7 cut(s)	1189, 1566, 1669, 2895, 3063, 3231, 3917
Bse3DI	GCAATG	3 cut(s)	1551, 1927, 3588
BseAI	TCCGGA	1 cut(s)	2006
BseBI	CCWGG	8 cut(s)	271, 1656, 1818, 2102, 2249, 2254, 3654, 4017
BseDI	CCNNGG	8 cut(s)	106, 1863, 2101, 2247, 2283, 2907, 3243, 3761
BseLI	CCNNNNNNNGG	6 cut(s)	206, 910, 1869, 2107, 2253, 3767
BseMI	GCAATG	3 cut(s)	1551, 1927, 3588
BseMII	CTCAG	6 cut(s)	915, 1016, 1311, 1374, 2217, 2424
BseNI	ACTGG	7 cut(s)	1189, 1566, 1669, 2895, 3063, 3231, 3917
BseRI	GAGGAG	5 cut(s)	1557, 1560, 2450, 3828, 3862
BseXI	GCAGC	8 cut(s)	671, 808, 1184, 2344, 2727, 3046, 3214, 3460
BseYI	CCCAGC	3 cut(s)	206, 1266, 1895
Bsh1285I	CGRYCG	2 cut(s)	2155, 4011
BshFI	GGCC	5 cut(s)	157, 2111, 2252, 3760, 4048
BsiEI	CGRYCG	2 cut(s)	2155, 4011
BsiHKAI	GWGCWC	1 cut(s)	2151
BsiHKCI	CYCGRG	1 cut(s)	2189
BsiSI	CCGG	5 cut(s)	1463, 1945, 2007, 2058, 2112
BslFI	GGGAC	1 cut(s)	343
BslI	CCNNNNNNNGG	6 cut(s)	206, 910, 1869, 2107, 2253, 3767
BsmBI	CGTCTC	4 cut(s)	50, 354, 2122, 3480
BsmFI	GGGAC	1 cut(s)	343
BsmI	GAATGC	2 cut(s)	2503, 3996
BsnI	GGCC	5 cut(s)	157, 2111, 2252, 3760, 4048
Bso31I	GGTCTC	1 cut(s)	865
BsoBI	CYCGRG	1 cut(s)	2189
Bsp1286I	GDGCHC	3 cut(s)	230, 1402, 2151
Bsp13I	TCCGGA	1 cut(s)	2006
Bsp1407I	TGTACA	4 cut(s)	787, 853, 1713, 1971
Bsp19I	CCATGG	1 cut(s)	106
BspANI	GGCC	5 cut(s)	157, 2111, 2252, 3760, 4048
BspCNI	CTCAG	6 cut(s)	916, 1015, 1312, 1375, 2218, 2425
BspEI	TCCGGA	1 cut(s)	2006
BspHI	TCATGA	1 cut(s)	3745
BspLI	GGNNCC	8 cut(s)	112, 144, 915, 1399, 1566, 2005, 2011, 2299
BspMAI	CTGCAG	2 cut(s)	366, 1170
BspMI	ACCTGC	3 cut(s)	11, 2830, 3454
BspTI	CTTAAG	1 cut(s)	2386
BspTNI	GGTCTC	1 cut(s)	865
BsrDI	GCAATG	3 cut(s)	1551, 1927, 3588
BsrFI	RCCGGY	2 cut(s)	2057, 2111
BsrGI	TGTACA	4 cut(s)	787, 853, 1713, 1971
BsrI	ACTGG	7 cut(s)	1189, 1566, 1669, 2895, 3063, 3231, 3917
BssAI	RCCGGY	2 cut(s)	2057, 2111
BssECI	CCNNGG	8 cut(s)	106, 1863, 2101, 2247, 2283, 2907, 3243, 3761
BssNAI	GTATAC	1 cut(s)	1446
BssNI	GRCGYC	3 cut(s)	347, 1160, 2115
BssSI	CACGAG	1 cut(s)	696
BssT1I	CCWWGG	2 cut(s)	106, 1863
Bst1107I	GTATAC	1 cut(s)	1446
Bst2BI	CACGAG	1 cut(s)	696
Bst2UI	CCWGG	8 cut(s)	271, 1656, 1818, 2102, 2249, 2254, 3654, 4017
Bst4CI	ACNGT	9 cut(s)	179, 499, 575, 794, 936, 1108, 1117, 1713, 4009
Bst6I	CTCTTC	9 cut(s)	924, 1940, 2618, 2780, 2996, 3164, 3660, 3732, 3839
BstACI	GRCGYC	3 cut(s)	347, 1160, 2115
BstAFI	CTTAAG	1 cut(s)	2386
BstAUI	TGTACA	4 cut(s)	787, 853, 1713, 1971
BstC8I	GCNNGC	7 cut(s)	366, 1170, 1693, 2113, 2159, 2244, 2483
BstDEI	CTNAG	6 cut(s)	924, 1002, 1320, 1383, 2226, 2433
BstDSI	CCRYGG	1 cut(s)	106
BstH2I	RGCGCY	1 cut(s)	2164
BstHHI	GCGC	3 cut(s)	1191, 1501, 2163
BstMCI	CGRYCG	2 cut(s)	2155, 4011
BstNI	CCWGG	8 cut(s)	271, 1656, 1818, 2102, 2249, 2254, 3654, 4017
BstNSI	RCATGY	4 cut(s)	79, 372, 1765, 3368
BstSCI	CCNGG	8 cut(s)	269, 1654, 1816, 2100, 2247, 2252, 3652, 4015
BstSFI	CTRYAG	4 cut(s)	362, 1104, 1166, 2418
BstV1I	GCAGC	8 cut(s)	671, 808, 1184, 2344, 2727, 3046, 3214, 3460
BstV2I	GAAGAC	4 cut(s)	297, 365, 1092, 3906
BstX2I	RGATCY	7 cut(s)	142, 297, 913, 2915, 3083, 3251, 3560
BstYI	RGATCY	7 cut(s)	142, 297, 913, 2915, 3083, 3251, 3560
BstZ17I	GTATAC	1 cut(s)	1446
BsuI	GTATCC	1 cut(s)	1021
BsuRI	GGCC	5 cut(s)	157, 2111, 2252, 3760, 4048
BtgI	CCRYGG	1 cut(s)	106
BtgZI	GCGATG	1 cut(s)	84
BtrI	CACGTC	1 cut(s)	3683
BtsI	GCAGTG	4 cut(s)	1163, 2301, 2668, 2725
BtsIMutI	CAGTG	9 cut(s)	1113, 1163, 2301, 2668, 2725, 2877, 2902, 3070, 3238
BveI	ACCTGC	3 cut(s)	11, 2830, 3454
Cac8I	GCNNGC	7 cut(s)	366, 1170, 1693, 2113, 2159, 2244, 2483
CciI	TCATGA	1 cut(s)	3745
CfoI	GCGC	3 cut(s)	1191, 1501, 2163
Cfr10I	RCCGGY	2 cut(s)	2057, 2111
Cfr13I	GGNCC	4 cut(s)	1145, 2004, 2297, 4013
CseI	GACGC	7 cut(s)	336, 710, 1149, 1292, 1802, 2104, 2231
CsiI	ACCWGGT	1 cut(s)	1654
Csp6I	GTAC	8 cut(s)	672, 788, 854, 1048, 1714, 1873, 1972, 2791
CspCI	CAANNNNNGTGG	2 cut(s)	2290, 2325
CviQI	GTAC	8 cut(s)	672, 788, 854, 1048, 1714, 1873, 1972, 2791
DdeI	CTNAG	6 cut(s)	924, 1002, 1320, 1383, 2226, 2433
DrdI	GACNNNNNNGTC	1 cut(s)	125
DriI	GACNNNNNGTC	1 cut(s)	4011
DseDI	GACNNNNNNGTC	1 cut(s)	125
EaeI	YGGCCR	2 cut(s)	155, 2109
Eam1104I	CTCTTC	9 cut(s)	924, 1940, 2618, 2780, 2996, 3164, 3660, 3732, 3839
Eam1105I	GACNNNNNGTC	1 cut(s)	4011
EarI	CTCTTC	9 cut(s)	924, 1940, 2618, 2780, 2996, 3164, 3660, 3732, 3839
EciI	GGCGGA	1 cut(s)	1928
Eco130I	CCWWGG	2 cut(s)	106, 1863
Eco24I	GRGCYC	2 cut(s)	230, 1402
Eco31I	GGTCTC	1 cut(s)	865
Eco47I	GGWCC	4 cut(s)	1145, 2004, 2297, 4013
Eco57I	CTGAAG	7 cut(s)	1197, 2433, 2757, 2835, 3003, 3171, 3345
Eco88I	CYCGRG	1 cut(s)	2189
EcoRI	GAATTC	2 cut(s)	544, 808
EcoRII	CCWGG	8 cut(s)	269, 1654, 1816, 2100, 2247, 2252, 3652, 4015
EcoT14I	CCWWGG	2 cut(s)	106, 1863
EcoT38I	GRGCYC	2 cut(s)	230, 1402
ErhI	CCWWGG	2 cut(s)	106, 1863
Esp3I	CGTCTC	4 cut(s)	50, 354, 2122, 3480
FalI	AAGNNNNNCTT	2 cut(s)	1713, 1745
FaqI	GGGAC	1 cut(s)	343
FbaI	TGATCA	1 cut(s)	3802
FblI	GTMKAC	3 cut(s)	442, 1203, 1445
FriOI	GRGCYC	2 cut(s)	230, 1402
GlaI	GCGC	3 cut(s)	1190, 1500, 2162
GsaI	CCCAGC	3 cut(s)	210, 1270, 1899
GsuI	CTGGAG	2 cut(s)	2065, 3934
HaeII	RGCGCY	1 cut(s)	2164
HaeIII	GGCC	5 cut(s)	157, 2111, 2252, 3760, 4048
HapII	CCGG	5 cut(s)	1463, 1945, 2007, 2058, 2112
HgaI	GACGC	7 cut(s)	336, 710, 1149, 1292, 1802, 2104, 2231
HhaI	GCGC	3 cut(s)	1191, 1501, 2163
Hin1I	GRCGYC	3 cut(s)	347, 1160, 2115
Hin6I	GCGC	3 cut(s)	1189, 1499, 2161
HinP1I	GCGC	3 cut(s)	1189, 1499, 2161
HincII	GTYRAC	4 cut(s)	508, 2827, 2995, 3163
HindII	GTYRAC	4 cut(s)	508, 2827, 2995, 3163
HindIII	AAGCTT	5 cut(s)	160, 1068, 2177, 2240, 4054
HpaI	GTTAAC	1 cut(s)	508
HpaII	CCGG	5 cut(s)	1463, 1945, 2007, 2058, 2112
Hpy99I	CGWCG	3 cut(s)	128, 131, 2225
HpyCH4III	ACNGT	9 cut(s)	179, 499, 575, 794, 936, 1108, 1117, 1713, 4009
HpyCH4IV	ACGT	5 cut(s)	84, 634, 888, 1586, 3682
HpyF3I	CTNAG	6 cut(s)	924, 1002, 1320, 1383, 2226, 2433
HpySE526I	ACGT	5 cut(s)	84, 634, 888, 1586, 3682
Hsp92I	GRCGYC	3 cut(s)	347, 1160, 2115
HspAI	GCGC	3 cut(s)	1189, 1499, 2161
Kpn2I	TCCGGA	1 cut(s)	2006
KroI	GCCGGC	1 cut(s)	2111
KroNI	GCCGGC	1 cut(s)	2113
Ksp22I	TGATCA	1 cut(s)	3802
KspAI	GTTAAC	1 cut(s)	508
Lsp1109I	GCAGC	8 cut(s)	671, 808, 1184, 2344, 2727, 3046, 3214, 3460
LweI	GCATC	8 cut(s)	194, 1303, 1898, 2383, 2707, 2893, 3229, 4029
MabI	ACCWGGT	1 cut(s)	1654
MaeII	ACGT	5 cut(s)	84, 634, 888, 1586, 3682
MfeI	CAATTG	2 cut(s)	1059, 1608
MflI	RGATCY	7 cut(s)	142, 297, 913, 2915, 3083, 3251, 3560
MhlI	GDGCHC	3 cut(s)	230, 1402, 2151
MlyI	GAGTC	6 cut(s)	44, 656, 1987, 2254, 3383, 3410
MmeI	TCCRAC	2 cut(s)	1111, 3301
MroI	TCCGGA	1 cut(s)	2006
MroNI	GCCGGC	1 cut(s)	2111
MroXI	GAANNNNTTC	2 cut(s)	310, 2036
MslI	CAYNNNNRTG	3 cut(s)	1311, 1506, 2637
MspA1I	CMGCKG	1 cut(s)	1175
MspCI	CTTAAG	1 cut(s)	2386
MspI	CCGG	5 cut(s)	1463, 1945, 2007, 2058, 2112
MspR9I	CCNGG	8 cut(s)	271, 1656, 1818, 2102, 2249, 2254, 3654, 4017
MunI	CAATTG	2 cut(s)	1059, 1608
Mva1269I	GAATGC	2 cut(s)	2503, 3996
MvaI	CCWGG	8 cut(s)	271, 1656, 1818, 2102, 2249, 2254, 3654, 4017
NaeI	GCCGGC	1 cut(s)	2113
NcoI	CCATGG	1 cut(s)	106
NgoMIV	GCCGGC	1 cut(s)	2111
NlaIV	GGNNCC	8 cut(s)	112, 144, 915, 1399, 1566, 2005, 2011, 2299
NmeAIII	GCCGAG	4 cut(s)	2098, 2932, 3268, 3786
NmuCI	GTSAC	7 cut(s)	23, 722, 966, 1161, 1465, 3671, 4024
NspI	RCATGY	4 cut(s)	79, 372, 1765, 3368
PaeR7I	CTCGAG	1 cut(s)	2189
PagI	TCATGA	1 cut(s)	3745
PaqCI	CACCTGC	1 cut(s)	11
PciI	ACATGT	2 cut(s)	1761, 3364
PcsI	WCGNNNNNNNCGW	1 cut(s)	123
PctI	GAATGC	2 cut(s)	2503, 3996
PdiI	GCCGGC	1 cut(s)	2113
PdmI	GAANNNNTTC	2 cut(s)	310, 2036
PflFI	GACNNNGTC	1 cut(s)	122
PflMI	CCANNNNNTGG	4 cut(s)	206, 910, 1869, 2107
PfoI	TCCNGGA	1 cut(s)	269
Ple19I	CGATCG	1 cut(s)	2155
PleI	GAGTC	6 cut(s)	43, 655, 1987, 2254, 3383, 3409
PpsI	GAGTC	6 cut(s)	43, 655, 1987, 2254, 3383, 3409
PscI	ACATGT	2 cut(s)	1761, 3364
PshBI	ATTAAT	1 cut(s)	1121
PsiI	TTATAA	1 cut(s)	1965
Psp6I	CCWGG	8 cut(s)	269, 1654, 1816, 2100, 2247, 2252, 3652, 4015
PspFI	CCCAGC	3 cut(s)	206, 1266, 1895
PspGI	CCWGG	8 cut(s)	269, 1654, 1816, 2100, 2247, 2252, 3652, 4015
PspN4I	GGNNCC	8 cut(s)	112, 144, 915, 1399, 1566, 2005, 2011, 2299
PspPI	GGNCC	4 cut(s)	1145, 2004, 2297, 4013
PspXI	VCTCGAGB	1 cut(s)	2189
PstI	CTGCAG	2 cut(s)	366, 1170
PsuI	RGATCY	7 cut(s)	142, 297, 913, 2915, 3083, 3251, 3560
PsyI	GACNNNGTC	1 cut(s)	122
PvuI	CGATCG	1 cut(s)	2155
PvuII	CAGCTG	1 cut(s)	1175
RsaI	GTAC	8 cut(s)	673, 789, 855, 1049, 1715, 1874, 1973, 2792
RsaNI	GTAC	8 cut(s)	672, 788, 854, 1048, 1714, 1873, 1972, 2791
RseI	CAYNNNNRTG	3 cut(s)	1311, 1506, 2637
Sau96I	GGNCC	4 cut(s)	1145, 2004, 2297, 4013
SbfI	CCTGCAGG	1 cut(s)	366
SchI	GAGTC	6 cut(s)	44, 656, 1987, 2254, 3383, 3410
ScrFI	CCNGG	8 cut(s)	271, 1656, 1818, 2102, 2249, 2254, 3654, 4017
SdaI	CCTGCAGG	1 cut(s)	366
SduI	GDGCHC	3 cut(s)	230, 1402, 2151
SexAI	ACCWGGT	1 cut(s)	1654
SfaNI	GCATC	8 cut(s)	194, 1303, 1898, 2383, 2707, 2893, 3229, 4029
SfcI	CTRYAG	4 cut(s)	362, 1104, 1166, 2418
Sfr274I	CTCGAG	1 cut(s)	2189
SinI	GGWCC	4 cut(s)	1145, 2004, 2297, 4013
SlaI	CTCGAG	1 cut(s)	2189
SmiMI	CAYNNNNRTG	3 cut(s)	1311, 1506, 2637
SmlI	CTYRAG	5 cut(s)	868, 2144, 2174, 2189, 2386
SmoI	CTYRAG	5 cut(s)	868, 2144, 2174, 2189, 2386
SpeI	ACTAGT	2 cut(s)	1150, 3620
Sse8387I	CCTGCAGG	1 cut(s)	366
SspI	AATATT	1 cut(s)	3640
StyD4I	CCNGG	8 cut(s)	269, 1654, 1816, 2100, 2247, 2252, 3652, 4015
StyI	CCWWGG	2 cut(s)	106, 1863
TaaI	ACNGT	9 cut(s)	179, 499, 575, 794, 936, 1108, 1117, 1713, 4009
TaiI	ACGT	5 cut(s)	87, 637, 891, 1589, 3685
TaqII	GACCGA	1 cut(s)	4001
TatI	WGTACW	5 cut(s)	671, 787, 853, 1713, 1971
TauI	GCSGC	9 cut(s)	7, 51, 1629, 2072, 2273, 2276, 2279, 2839, 3474
TscAI	CASTG	9 cut(s)	1120, 1170, 2308, 2668, 2725, 2884, 2902, 3070, 3238
TseFI	GTSAC	7 cut(s)	23, 722, 966, 1161, 1465, 3671, 4024
TseI	GCWGC	8 cut(s)	659, 821, 1172, 2357, 2715, 3059, 3227, 3448
Tsp45I	GTSAC	7 cut(s)	23, 722, 966, 1161, 1465, 3671, 4024
TspGWI	ACGGA	4 cut(s)	74, 640, 1992, 3690
TspRI	CASTG	9 cut(s)	1120, 1170, 2308, 2668, 2725, 2884, 2902, 3070, 3238
Tth111I	GACNNNGTC	1 cut(s)	122
Van91I	CCANNNNNTGG	4 cut(s)	206, 910, 1869, 2107
Vha464I	CTTAAG	1 cut(s)	2386
VpaK11BI	GGWCC	4 cut(s)	1145, 2004, 2297, 4013
VspI	ATTAAT	1 cut(s)	1121
XapI	RAATTY	5 cut(s)	544, 622, 808, 1406, 3272
XbaI	TCTAGA	2 cut(s)	994, 3859
XceI	RCATGY	4 cut(s)	79, 372, 1765, 3368
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1120
XhoI	CTCGAG	1 cut(s)	2189
XmiI	GTMKAC	3 cut(s)	442, 1203, 1445
XmnI	GAANNNNTTC	2 cut(s)	310, 2036

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.