Rorug06G0395500

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0002236
Physical Location & Seq
Forward (+)
54084000 .. 54091764
7765 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0395500.1

Sequence Viewer

Length: 4095 bp
ATGTGGAGAAACGAGGATACTTCAATGGAAGCTTTCTCCAAGTCTTCTCGTGAAGAAGACGATGAAGAATCTCTCAAATGGGCTGCTATTGAGAGATTGCCTACTTATTTACGCATAAGGAGAAGTATACTCGCTGATCAAGAAGAGGGTAAAGCCAGAGAAATTGATGTCAAGGATCTTGGATTACTAGAGAGGAAGAATTTGTTGGAGAGGTTAGTGAAGATTGCTGAGGAAGATAATGAGAAATTCTTGTTCAAGCTCAGGGACCGCATGAATAGAGTTGGACTTGAATTTCCAACAACTGAAGTGCGGTTCGAGCACTTAAATGTTGAAGCAGAAGCTTATGTAGGAAGCAGGGCATTACCTACAATGTTCAACTTCTCCATTAATATGTTAGAGGGGCTCTTGAATTGTGTTCATCTTCTTCCTAGTAGAAAGAAACCTTTGCCAATCCTACATGGTGTAAGTGGAATAATCAAGCCAAGAAGAATGACACTGCTTTTAGGCCCTCCAGGCTCCGGAAAGACCACATTGCTATTGGCATTGGCTGGAAAGCTTGGTAAAGATCTTAAAAGATCTGGAAGAGTTACCTACAATGGACTTGGGATGGATGAGTTTGTACCAGAGAGGACAGCAGCTTACATTAGTCAATATGATCTTCACATACCAGAGTTGACTGTGAGAGAAACTCTAGCTTTTTCAGCAAGATGTCAAGGGGTCGGACCACGTTATGAAATGTTAGTAGAACTACTAAGAAGAGAGAAGGCTGCAACTATTATGCCAGATTCTGATCTTGATGTCTACATGAAGGCAGCAGCACTAAAAGGACAGGAAACCACTGTGATTACAGATTATATACTCAAGGTTTTGGGACTTGAAGTTTGTGCAGACACCATGGTAGGGGATCAAATGAGACGAGGTATATCTGGTGGCCAAAGAAAGCGACTCACCACAGGGGAAATGCTTGTTGGGCCAGCAAGAGCACTTTTTATGGATGAGATATCAACTGGCCTGGACAGTTCAACAACATTTCAGATAGTAAATTCACTACGACAATCCATTCACATTCTCAATGGAACTGCTGTAATCTCTCTCCTGCAGCCAGCACCAGAAACTTATGATCTCTTTGATGATATAATTCTCCTCTCAGATGGGCACATTGTGTATCAAGGTCCTCGCGAAAATGTGCTTGAGTTCTTTGAGTACATGGGCTTCCGATGTCCAGAGAGGAAAGGAGTCGCTGATTTCCTCCAAGAAGTGACATCATGGAAAGACCAAGAGCAATATTGGACCGATAAAGATAAGCCTTATAGCTTTGTCACGTCCAAGGAATTTTCTGAAGCATTGCAGTCATTTCACATTGGTCAAGAACTCGGTGATGAGCTTGCTAGACCTTTTGACAAGTCTAGAGGAAACCCTGCATCTTTAACAACTGAGAAGTACGGCGTTAGCAAGAAGGAACTTTTTAAAGCTTGTCTGTCTAGACATTATTTGCTCATGAAGAGGAACTCGTTTGTCTACATTACCAGAATGACAATGTTTCTCATAACTGCTTTTGTGACAATGACACTATTCCTACGGACTGAGATGCACCGGGATACAGTAGAAGATGGTGGCATTTATATGGGAGCTATGTTCTTCACAATGATGGTAATTATGTTCAATGGATACTCAGACCTTGCTATGACTCTCATGAAACTTCCTGTGTTTTACAAGCAAAGGGACCTGCTCTTCTATCCTGCTTGGGCATACTCTTTACCAACAGTGATTATAAGAATCCCCATGACCTTTGTGGAAGTTTCCATTTGGATGGCCATCACTTACTACGGTATTGGTTATGATCCAAGCATTGGAAGGTTCTGCAAGCAGTTCCTTGTACTAGTGTGCATTAGCCAGATGGCAAACGGACTGTTCCGATTGATAGGTGCATTAGGAAGGAACATGACTGTTGCAAACACATTTGGATCTGTTGCTTTACTATGCTGTTTTGTTCTGGGTGGCTTTATGTTATCACGAGAGGAGATGAAGAACTGGTTGTTGTGGGGATATTGGCTCTCACCGATGACGTATGGAACGGATGCAATGGCTGTCAATGAATTTTTTGGAAAAAGTTGGAGACATGTTCCTCCCAACTCAACAGAATCGTTAGGAGTTATGGTCTTGAAGTCTCGAGGGGTATTCCCAGATGCAAGTTGGTACTGGATTGGAGTAGTAGCTTTATTTGGATATTTTCTTCTGTTCAACTTCCTTTACACTTTGGCACTTCAGCTTCTGGAACCACTTGATAAGCCTCAGGCAGTATTGTCCAAAGAGGCTTTGGCAGTGACACACGCCATGAAAACTGGAGAGACCATTGAGCTATCGCCAACAGGAAACAATTCTATTGGAGTAGGGAATGAAGTTCTAGAGAATGTGTCAGCCAATCGTCCCATGCAGCAAGGAATGATTCTTCCTTTTCAACCCCTCTCAATTACTTTTACTGAAATCAGATATGCAGTTGACATGCCACAGGAAATGAAAGATCAAGGCATTACTGAGGATCGACTAGAACTGCTGAAGGGTGTGAGTGGTGCTTTCAGGCCTGGGGTCCTAACAGCTCTAATGGGTGTTAGCGGTGCTGGAAAGACTACTTTACTGGATGTCCTGGCAGGAAGGAAAACCGGTGGATATATTGAAGGAAACATTACCATTTCTGGGTATCCAAAAAAGCAAGAAACATTTGCTCGTATCTCAGGATATTGTGAGCAGACAGATATACACTCTCCACATGTTACGGTTTATGAGTCTTTGATTTATTCTGCATGGCTCCGGTTGTCCCCTGAGGTTGATTCTCCAACCAGAAAGATGTTTATTGAAGAAGTCATGGAACTTGTGGAACTGACCTCAATTAGGGAAGCACTTGTTGGATTGACTGGAGGGAATGGTCTCTCGACTGAGCAGCGCAAAAGACTAACAATTGCAGTAGAGCTTGTTGCCAACCCATCCATAATATTTATGGATGAGCCCACCTCTGGCCTTGATGCCAGGGCAGCAGCAATTGTAATGAGAACAGTGAGGAATACAGTGGATACCGGAAGAACAGTAGTCTGCACCATCCACCAGCCAAGCATTGATATATTTGATGCTTTTGATGAGATGCTCCTTCTGAAACAGGGTGGCGAAGAAATATACGTTGGCCCTTTAGGCTACCATTCTTCCTACTTGATCGGATACTTTGAGGGAATTAGTGGAGTTTCTAAAATGAAGAATGGTTACAATCCTGCAACTTGGATGTTGGAGGTTACTTCTGCAGCTCAAGAAGCAGCTCTAGGGATTAATTTCACAGACATATACAAGAATTCAGAAGCATACAGGAGAAACAAGGCCTTGGTCAAGGAACTAAGTATTCCTCCACCGAATACAAGAGACTTGTACTTCCCAACAAAATATTCTCAGTCTTTCTATACCCAGTGCAAAGCTTGCTTATGGAAGCAACATTTGTCTTACTGGAGAAACCAGGCATACAGTGCAGTTAGACTTCTTTTCACAACATTGATAGCTTTAGCAACCGGGACATTATTCTGGGGTCTTGGCTCCAGAAGGCAAAAGCAGCAAGATCTTTTTAACGCAATGGGTGCTATGTATTCTGCTATTCTCTTCATTGGGCCACAAAATACCTCAACAGTGCAGCCAGTTGTAGGCATTGAGCGAATAGTCTTTTACAGAGAAAGGGCTGCCGGAATGTACTCTGCCTTGCCATATGCCTTTGGACAGGTTGGCATTGAGCTCCCATACACTTTGATCCAAACTATCATATATGTGGTTATATCATACACGATGATCGGATTTGAGTGGACGAGAATTCCAATATGGTGGAGATGGTTCTACTGGTCGTGCCCAGTCTCTTGGAGCTTGTACGGAATGATTGGTTCGCAGTTTGGAGGCATTGAGGACAGGCTTGATTCCGGCGAAACTGTGGACGCTTTTACCAGGACTTATTTTGGGTATAGAGAAGACTTTATGAGTGTTGTTGCAATAGTGCTCGTAGCTTTTTCAGTGCTCTTTGGATCCGTCTTTGCCTTTACAATCAAAGCCTTTAACTTTCAAAAGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

1364

Amino Acids

154.03

Weight (kDa)

6.35

Isoelectric Point (pI)

39.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_trans_N PF14510 77 - 127 1.8e-07 ABC-transporter N-terminal
ABC_tran PF00005 152 - 333 3.3e-15 ABC transporter
ABC2_membrane PF01061 488 - 700 2.5e-49 ABC-2 type transporter
PDR_assoc PF08370 705 - 767 2.9e-26 Plant PDR ABC transporter associated
ABC_tran PF00005 852 - 1004 8.4e-21 ABC transporter
ABC2_membrane PF01061 1149 - 1279 5.6e-34 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1772
Acc36I ACCTGC 1 cut(s) 1734
AccB7I CCANNNNNTGG 1 cut(s) 1850
AccI GTMKAC 3 cut(s) 127, 801, 1518
AccII CGCG 1 cut(s) 1179
AccIII TCCGGA 1 cut(s) 518
AciI CCGC 3 cut(s) 268, 310, 2613
AclWI GGATC 8 cut(s) 183, 912, 1835, 1972, 2547, 3775, 4041, 4054
AcoI YGGCCR 2 cut(s) 931, 1812
AcsI RAATTY 8 cut(s) 199, 245, 290, 1042, 1331, 2096, 3337, 3840
AcuI CTGAAG 4 cut(s) 324, 1359, 2249, 2576
AdeI CACNNNGTG 1 cut(s) 1162
AfaI GTAC 8 cut(s) 621, 1205, 1442, 1878, 2198, 3413, 3725, 3896
AfiI CCNNNNNNNGG 7 cut(s) 518, 900, 1850, 2694, 2889, 3011, 3677
AflIII ACRYGT 2 cut(s) 2119, 2767
AgeI ACCGGT 1 cut(s) 2660
AhlI ACTAGT 1 cut(s) 1879
AjiI CACGTC 1 cut(s) 1323
AjnI CCWGG 7 cut(s) 511, 1011, 2581, 2643, 3023, 3495, 3968
Alw21I GWGCWC 5 cut(s) 321, 985, 3768, 4023, 4041
Alw26I GTCTC 7 cut(s) 907, 2110, 2172, 2342, 2930, 3399, 3886
AlwI GGATC 8 cut(s) 183, 912, 1835, 1972, 2547, 3775, 4041, 4054
AlwNI CAGNNNCTG 2 cut(s) 788, 2272
Ama87I CYCGRG 1 cut(s) 2169
Aor13HI TCCGGA 1 cut(s) 518
ApoI RAATTY 8 cut(s) 199, 245, 290, 1042, 1331, 2096, 3337, 3840
ArsI GACNNNNNNTTYG 2 cut(s) 864, 896
AseI ATTAAT 2 cut(s) 387, 3315
AsiGI ACCGGT 1 cut(s) 2660
Asp700I GAANNNNTTC 1 cut(s) 2377
AspLEI GCGC 1 cut(s) 2943
AsuC2I CCSGG 2 cut(s) 1595, 3550
AsuHPI GGTGA 3 cut(s) 940, 1388, 2049
AvaI CYCGRG 1 cut(s) 2169
AvaII GGWCC 6 cut(s) 265, 722, 1172, 1290, 1723, 2587
AxyI CCTNAGG 2 cut(s) 2292, 2820
BaeGI GKGCMC 2 cut(s) 1158, 3878
BalI TGGCCA 2 cut(s) 933, 1814
BamHI GGATCC 1 cut(s) 4046
BanII GRGCYC 3 cut(s) 405, 3006, 3768
BarI GAAGNNNNNNTAC 4 cut(s) 1196, 1228, 3236, 3268
BauI CACGAG 2 cut(s) 48, 2013
BbsI GAAGAC 3 cut(s) 36, 63, 3999
Bbv12I GWGCWC 5 cut(s) 321, 985, 3768, 4023, 4041
BbvCI CCTCAGC 1 cut(s) 228
BceAI ACGGC 1 cut(s) 1459
BciT130I CCWGG 7 cut(s) 513, 1013, 2583, 2645, 3025, 3497, 3970
BciVI GTATCC 6 cut(s) 10, 1591, 1661, 2709, 3061, 3203
BclI TGATCA 1 cut(s) 136
BcnI CCSGG 2 cut(s) 1595, 3550
BcoDI GTCTC 7 cut(s) 907, 2110, 2172, 2342, 2930, 3399, 3886
BcuI ACTAGT 1 cut(s) 1879
BfmI CTRYAG 2 cut(s) 1097, 3288
BfuAI ACCTGC 1 cut(s) 1734
BfuI GTATCC 6 cut(s) 10, 1591, 1661, 2709, 3061, 3203
BglI GCCNNNNNGGC 2 cut(s) 513, 3183
BglII AGATCT 3 cut(s) 565, 575, 3595
Bme1390I CCNGG 9 cut(s) 513, 1013, 1595, 2583, 2645, 3025, 3497, 3550, 3970
Bme18I GGWCC 6 cut(s) 265, 722, 1172, 1290, 1723, 2587
BmeT110I CYCGRG 1 cut(s) 2169
BmgBI CACGTC 1 cut(s) 1323
BmiI GGNNCC 8 cut(s) 266, 517, 1724, 2277, 2588, 2807, 3574, 4048
BmrFI CCNGG 9 cut(s) 513, 1013, 1595, 2583, 2645, 3025, 3497, 3550, 3970
BmrI ACTGGG 2 cut(s) 3442, 3872
BmsI GCATC 7 cut(s) 1430, 1578, 2068, 2176, 3010, 3112, 3126
BmuI ACTGGG 2 cut(s) 3442, 3872
BpiI GAAGAC 3 cut(s) 36, 63, 3999
BplI GAGNNNNNCTC 4 cut(s) 673, 705, 2993, 3025
BpmI CTGGAG 5 cut(s) 495, 2364, 2934, 3508, 3559
Bpu10I CCTNAGC 2 cut(s) 228, 260
BpuEI CTTGAG 3 cut(s) 845, 1211, 3279
BpuMI CCSGG 2 cut(s) 1595, 3550
BsaBI GATNNNNATC 2 cut(s) 789, 1814
BsaI GGTCTC 2 cut(s) 2342, 2930
BsaJI CCNNGG 5 cut(s) 894, 1326, 2582, 3024, 3366
BsaWI WCCGGW 4 cut(s) 518, 2660, 2808, 3071
BsaXI ACNNNNNCTCC 2 cut(s) 2142, 2172
Bsc4I CCNNNNNNNGG 7 cut(s) 518, 900, 1850, 2694, 2889, 3011, 3677
Bse118I RCCGGY 1 cut(s) 2660
Bse21I CCTNAGG 2 cut(s) 2292, 2820
Bse3DI GCAATG 4 cut(s) 530, 1343, 2088, 3615
Bse8I GATNNNNATC 2 cut(s) 789, 1814
BseAI TCCGGA 1 cut(s) 518
BseBI CCWGG 7 cut(s) 513, 1013, 2583, 2645, 3025, 3497, 3970
BseDI CCNNGG 5 cut(s) 894, 1326, 2582, 3024, 3366
BseJI GATNNNNATC 2 cut(s) 789, 1814
BseLI CCNNNNNNNGG 7 cut(s) 518, 900, 1850, 2694, 2889, 3011, 3677
BseMI GCAATG 4 cut(s) 530, 1343, 2088, 3615
BseRI GAGGAG 2 cut(s) 1133, 2033
BseSI GKGCMC 2 cut(s) 1158, 3878
BsgI GTGCAG 4 cut(s) 906, 3073, 3528, 3686
Bsh1236I CGCG 1 cut(s) 1179
BshTI ACCGGT 1 cut(s) 2660
BsiHKAI GWGCWC 5 cut(s) 321, 985, 3768, 4023, 4041
BsiHKCI CYCGRG 1 cut(s) 2169
BsiSI CCGG 8 cut(s) 519, 1594, 2661, 2809, 3072, 3549, 3717, 3945
BslFI GGGAC 6 cut(s) 278, 885, 1736, 2412, 2800, 3565
BslI CCNNNNNNNGG 7 cut(s) 518, 900, 1850, 2694, 2889, 3011, 3677
BsmAI GTCTC 7 cut(s) 907, 2110, 2172, 2342, 2930, 3399, 3886
BsmBI CGTCTC 1 cut(s) 907
BsmFI GGGAC 6 cut(s) 278, 885, 1736, 2412, 2800, 3565
Bso31I GGTCTC 2 cut(s) 2342, 2930
BsoBI CYCGRG 1 cut(s) 2169
Bsp1286I GDGCHC 9 cut(s) 321, 405, 985, 1158, 3006, 3768, 3878, 4023, 4041
Bsp13I TCCGGA 1 cut(s) 518
Bsp19I CCATGG 1 cut(s) 894
Bsp68I TCGCGA 1 cut(s) 1179
BspACI CCGC 3 cut(s) 268, 310, 2613
BspEI TCCGGA 1 cut(s) 518
BspFNI CGCG 1 cut(s) 1179
BspHI TCATGA 2 cut(s) 1497, 1692
BspLI GGNNCC 8 cut(s) 266, 517, 1724, 2277, 2588, 2807, 3574, 4048
BspMAI CTGCAG 2 cut(s) 1101, 3292
BspMI ACCTGC 1 cut(s) 1734
BspPI GGATC 8 cut(s) 183, 912, 1835, 1972, 2547, 3775, 4041, 4054
BspQI GCTCTTC 1 cut(s) 1736
BspTNI GGTCTC 2 cut(s) 2342, 2930
BsrDI GCAATG 4 cut(s) 530, 1343, 2088, 3615
BsrFI RCCGGY 1 cut(s) 2660
BssAI RCCGGY 1 cut(s) 2660
BssECI CCNNGG 5 cut(s) 894, 1326, 2582, 3024, 3366
BssNAI GTATAC 1 cut(s) 128
BssSI CACGAG 2 cut(s) 48, 2013
BssT1I CCWWGG 3 cut(s) 894, 1326, 3366
Bst1107I GTATAC 1 cut(s) 128
Bst2BI CACGAG 2 cut(s) 48, 2013
Bst2UI CCWGG 7 cut(s) 513, 1013, 2583, 2645, 3025, 3497, 3970
Bst6I CTCTTC 6 cut(s) 138, 577, 751, 1496, 1736, 3641
BstAPI GCANNNNNTGC 3 cut(s) 3459, 3506, 3614
BstC8I GCNNGC 5 cut(s) 975, 1104, 1386, 1865, 3460
BstDSI CCRYGG 1 cut(s) 894
BstENI CCTNNNNNAGG 1 cut(s) 2887
BstFNI CGCG 1 cut(s) 1179
BstHHI GCGC 1 cut(s) 2943
BstMAI GTCTC 7 cut(s) 907, 2110, 2172, 2342, 2930, 3399, 3886
BstNI CCWGG 7 cut(s) 513, 1013, 2583, 2645, 3025, 3497, 3970
BstNSI RCATGY 3 cut(s) 2123, 2506, 2771
BstSCI CCNGG 9 cut(s) 511, 1011, 1593, 2581, 2643, 3023, 3495, 3548, 3968
BstSFI CTRYAG 2 cut(s) 1097, 3288
BstSLI GKGCMC 2 cut(s) 1158, 3878
BstUI CGCG 1 cut(s) 1179
BstV2I GAAGAC 3 cut(s) 36, 63, 3999
BstX2I RGATCY 6 cut(s) 175, 565, 575, 1964, 3595, 4046
BstXI CCANNNNNNTGG 3 cut(s) 1810, 3852, 3885
BstYI RGATCY 6 cut(s) 175, 565, 575, 1964, 3595, 4046
BstZ17I GTATAC 1 cut(s) 128
Bsu36I CCTNAGG 2 cut(s) 2292, 2820
BsuI GTATCC 6 cut(s) 10, 1591, 1661, 2709, 3061, 3203
BtgI CCRYGG 1 cut(s) 894
BtrI CACGTC 1 cut(s) 1323
BtsI GCAGTG 2 cut(s) 494, 2328
BtuMI TCGCGA 1 cut(s) 1179
BveI ACCTGC 1 cut(s) 1734
Cac8I GCNNGC 5 cut(s) 975, 1104, 1386, 1865, 3460
CaiI CAGNNNCTG 2 cut(s) 788, 2272
CciI TCATGA 2 cut(s) 1497, 1692
CfoI GCGC 1 cut(s) 2943
Cfr10I RCCGGY 1 cut(s) 2660
CseI GACGC 1 cut(s) 3968
Csp6I GTAC 8 cut(s) 620, 1204, 1441, 1877, 2197, 3412, 3724, 3895
CspAI ACCGGT 1 cut(s) 2660
CviQI GTAC 8 cut(s) 620, 1204, 1441, 1877, 2197, 3412, 3724, 3895
DraI TTTAAA 1 cut(s) 1468
DraIII CACNNNGTG 1 cut(s) 1162
EaeI YGGCCR 2 cut(s) 931, 1812
Eam1104I CTCTTC 6 cut(s) 138, 577, 751, 1496, 1736, 3641
EarI CTCTTC 6 cut(s) 138, 577, 751, 1496, 1736, 3641
Ecl136II GAGCTC 1 cut(s) 3766
Eco130I CCWWGG 3 cut(s) 894, 1326, 3366
Eco147I AGGCCT 2 cut(s) 2581, 3365
Eco24I GRGCYC 3 cut(s) 405, 3006, 3768
Eco31I GGTCTC 2 cut(s) 2342, 2930
Eco32I GATATC 1 cut(s) 1002
Eco47I GGWCC 6 cut(s) 265, 722, 1172, 1290, 1723, 2587
Eco53kI GAGCTC 1 cut(s) 3766
Eco57I CTGAAG 4 cut(s) 324, 1359, 2249, 2576
Eco81I CCTNAGG 2 cut(s) 2292, 2820
Eco88I CYCGRG 1 cut(s) 2169
EcoICRI GAGCTC 1 cut(s) 3766
EcoNI CCTNNNNNAGG 1 cut(s) 2887
EcoO109I RGGNCCY 4 cut(s) 506, 1172, 1723, 2587
EcoRI GAATTC 2 cut(s) 3337, 3840
EcoRII CCWGG 7 cut(s) 511, 1011, 2581, 2643, 3023, 3495, 3968
EcoRV GATATC 1 cut(s) 1002
EcoT14I CCWWGG 3 cut(s) 894, 1326, 3366
EcoT38I GRGCYC 3 cut(s) 405, 3006, 3768
ErhI CCWWGG 3 cut(s) 894, 1326, 3366
Esp3I CGTCTC 1 cut(s) 907
FaqI GGGAC 6 cut(s) 278, 885, 1736, 2412, 2800, 3565
FauNDI CATATG 1 cut(s) 3739
FbaI TGATCA 1 cut(s) 136
FblI GTMKAC 3 cut(s) 127, 801, 1518
FriOI GRGCYC 3 cut(s) 405, 3006, 3768
GlaI GCGC 1 cut(s) 2942
GsuI CTGGAG 5 cut(s) 495, 2364, 2934, 3508, 3559
HapII CCGG 8 cut(s) 519, 1594, 2661, 2809, 3072, 3549, 3717, 3945
HgaI GACGC 1 cut(s) 3968
HhaI GCGC 1 cut(s) 2943
Hin6I GCGC 1 cut(s) 2941
HinP1I GCGC 1 cut(s) 2941
HincII GTYRAC 2 cut(s) 675, 2500
HindII GTYRAC 2 cut(s) 675, 2500
HindIII AAGCTT 5 cut(s) 30, 339, 554, 1470, 3456
HpaII CCGG 8 cut(s) 519, 1594, 2661, 2809, 3072, 3549, 3717, 3945
HphI GGTGA 3 cut(s) 940, 1388, 2049
Hpy166II GTNNAC 7 cut(s) 128, 675, 802, 1519, 2500, 3834, 3958
Hpy8I GTNNAC 7 cut(s) 128, 675, 802, 1519, 2500, 3834, 3958
HpyCH4IV ACGT 4 cut(s) 727, 1322, 2066, 3171
HpySE526I ACGT 4 cut(s) 727, 1322, 2066, 3171
HspAI GCGC 1 cut(s) 2941
Kpn2I TCCGGA 1 cut(s) 518
Ksp22I TGATCA 1 cut(s) 136
LguI GCTCTTC 1 cut(s) 1736
LmnI GCTCC 7 cut(s) 521, 1628, 2811, 3144, 3578, 3771, 3888
LweI GCATC 7 cut(s) 1430, 1578, 2068, 2176, 3010, 3112, 3126
MaeII ACGT 4 cut(s) 727, 1322, 2066, 3171
MaeIII GTNAC 8 cut(s) 586, 1258, 1318, 1558, 2323, 2770, 3251, 3280
MfeI CAATTG 2 cut(s) 2955, 3036
MflI RGATCY 6 cut(s) 175, 565, 575, 1964, 3595, 4046
MhlI GDGCHC 9 cut(s) 321, 405, 985, 1158, 3006, 3768, 3878, 4023, 4041
MlsI TGGCCA 2 cut(s) 933, 1814
MluNI TGGCCA 2 cut(s) 933, 1814
MlyI GAGTC 4 cut(s) 939, 1245, 1681, 2792
MmeI TCCRAC 8 cut(s) 186, 262, 320, 700, 2093, 2858, 2884, 3255
Mox20I TGGCCA 2 cut(s) 933, 1814
MroI TCCGGA 1 cut(s) 518
MroXI GAANNNNTTC 1 cut(s) 2377
MscI TGGCCA 2 cut(s) 933, 1814
MseI TTAA 8 cut(s) 323, 387, 570, 1427, 1467, 3315, 3603, 4077
MslI CAYNNNNRTG 6 cut(s) 324, 389, 1622, 1646, 1808, 3797
Msp20I TGGCCA 2 cut(s) 933, 1814
MspI CCGG 8 cut(s) 519, 1594, 2661, 2809, 3072, 3549, 3717, 3945
MspR9I CCNGG 9 cut(s) 513, 1013, 1595, 2583, 2645, 3025, 3497, 3550, 3970
MunI CAATTG 2 cut(s) 2955, 3036
MvaI CCWGG 7 cut(s) 513, 1013, 2583, 2645, 3025, 3497, 3970
MvnI CGCG 1 cut(s) 1179
NciI CCSGG 2 cut(s) 1595, 3550
NcoI CCATGG 1 cut(s) 894
NdeI CATATG 1 cut(s) 3739
NlaIV GGNNCC 8 cut(s) 266, 517, 1724, 2277, 2588, 2807, 3574, 4048
NmuCI GTSAC 4 cut(s) 1258, 1318, 1558, 2323
NruI TCGCGA 1 cut(s) 1179
NspI RCATGY 3 cut(s) 2123, 2506, 2771
PaeR7I CTCGAG 1 cut(s) 2169
PagI TCATGA 2 cut(s) 1497, 1692
PceI AGGCCT 2 cut(s) 2581, 3365
PciI ACATGT 2 cut(s) 2119, 2767
PciSI GCTCTTC 1 cut(s) 1736
PdmI GAANNNNTTC 1 cut(s) 2377
PfeI GAWTC 7 cut(s) 68, 785, 1776, 2141, 2446, 2828, 3941
PflMI CCANNNNNTGG 1 cut(s) 1850
PinAI ACCGGT 1 cut(s) 2660
PleI GAGTC 4 cut(s) 939, 1244, 1681, 2791
PpsI GAGTC 4 cut(s) 939, 1244, 1681, 2791
PpuMI RGGWCCY 3 cut(s) 1172, 1723, 2587
PscI ACATGT 2 cut(s) 2119, 2767
PshBI ATTAAT 2 cut(s) 387, 3315
PsiI TTATAA 1 cut(s) 1772
Psp124BI GAGCTC 1 cut(s) 3768
Psp5II RGGWCCY 3 cut(s) 1172, 1723, 2587
Psp6I CCWGG 7 cut(s) 511, 1011, 2581, 2643, 3023, 3495, 3968
PspGI CCWGG 7 cut(s) 511, 1011, 2581, 2643, 3023, 3495, 3968
PspN4I GGNNCC 8 cut(s) 266, 517, 1724, 2277, 2588, 2807, 3574, 4048
PspPPI RGGWCCY 3 cut(s) 1172, 1723, 2587
PstI CTGCAG 2 cut(s) 1101, 3292
PstNI CAGNNNCTG 2 cut(s) 788, 2272
PsuI RGATCY 6 cut(s) 175, 565, 575, 1964, 3595, 4046
RruI TCGCGA 1 cut(s) 1179
RsaI GTAC 8 cut(s) 621, 1205, 1442, 1878, 2198, 3413, 3725, 3896
RsaNI GTAC 8 cut(s) 620, 1204, 1441, 1877, 2197, 3412, 3724, 3895
RseI CAYNNNNRTG 6 cut(s) 324, 389, 1622, 1646, 1808, 3797
SacI GAGCTC 1 cut(s) 3768
SapI GCTCTTC 1 cut(s) 1736
SaqAI TTAA 8 cut(s) 323, 387, 570, 1427, 1467, 3315, 3603, 4077
SchI GAGTC 4 cut(s) 939, 1245, 1681, 2792
ScrFI CCNGG 9 cut(s) 513, 1013, 1595, 2583, 2645, 3025, 3497, 3550, 3970
SduI GDGCHC 9 cut(s) 321, 405, 985, 1158, 3006, 3768, 3878, 4023, 4041
SfaNI GCATC 7 cut(s) 1430, 1578, 2068, 2176, 3010, 3112, 3126
SfcI CTRYAG 2 cut(s) 1097, 3288
Sfr274I CTCGAG 1 cut(s) 2169
SinI GGWCC 6 cut(s) 265, 722, 1172, 1290, 1723, 2587
SlaI CTCGAG 1 cut(s) 2169
SmiMI CAYNNNNRTG 6 cut(s) 324, 389, 1622, 1646, 1808, 3797
SmlI CTYRAG 4 cut(s) 860, 1190, 2169, 3294
SmoI CTYRAG 4 cut(s) 860, 1190, 2169, 3294
SpeI ACTAGT 1 cut(s) 1879
SseBI AGGCCT 2 cut(s) 2581, 3365
SsiI CCGC 3 cut(s) 268, 310, 2613
SspI AATATT 3 cut(s) 1286, 2991, 3428
SstI GAGCTC 1 cut(s) 3768
StuI AGGCCT 2 cut(s) 2581, 3365
StyD4I CCNGG 9 cut(s) 511, 1011, 1593, 2581, 2643, 3023, 3495, 3548, 3968
StyI CCWWGG 3 cut(s) 894, 1326, 3366
TaiI ACGT 4 cut(s) 730, 1325, 2069, 3174
TaqI TCGA 4 cut(s) 315, 2170, 2542, 2930
TaqII GACCGA 1 cut(s) 1307
TatI WGTACW 4 cut(s) 1203, 1876, 3411, 3723
TfiI GAWTC 7 cut(s) 68, 785, 1776, 2141, 2446, 2828, 3941
Tru1I TTAA 8 cut(s) 323, 387, 570, 1427, 1467, 3315, 3603, 4077
Tru9I TTAA 8 cut(s) 323, 387, 570, 1427, 1467, 3315, 3603, 4077
TseFI GTSAC 4 cut(s) 1258, 1318, 1558, 2323
Tsp45I GTSAC 4 cut(s) 1258, 1318, 1558, 2323
TspGWI ACGGA 5 cut(s) 1594, 1920, 2090, 3912, 4039
Van91I CCANNNNNTGG 1 cut(s) 1850
VpaK11BI GGWCC 6 cut(s) 265, 722, 1172, 1290, 1723, 2587
VspI ATTAAT 2 cut(s) 387, 3315
XagI CCTNNNNNAGG 1 cut(s) 2887
XapI RAATTY 8 cut(s) 199, 245, 290, 1042, 1331, 2096, 3337, 3840
XbaI TCTAGA 3 cut(s) 1406, 1481, 2404
XceI RCATGY 3 cut(s) 2123, 2506, 2771
XcmI CCANNNNNNNNNTGG 5 cut(s) 535, 1789, 2190, 2314, 2992
XhoI CTCGAG 1 cut(s) 2169
XmiI GTMKAC 3 cut(s) 127, 801, 1518
XmnI GAANNNNTTC 1 cut(s) 2377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.