pycom08g11180

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0002236
Physical Location & Seq
Forward (+)
9477518 .. 9479164
1647 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g11180.1

Sequence Viewer

Length: 606 bp
ATGGGTGTGACCGTGAAGCAGATGTCTCTGACAGTGGCACTTTTCGGTGTTCTCTCGTTCATATTCGGAGTTATTGCTGAAAACAAGAAGCCAGCAGCTGGAACTCCTATGGTTGGGAAAGATGTTGTGATCTGCAAGTATCCTTCAGATCCATCAGTTGTCTTTGGGTATCTGTCTTTTGCTTTCCTCCTGGTATCTACAGTTGCTGGGTTCTTGTCATTGTTTTACCCATACAAAGGGAAGTCCCTTCCACAAGCTGCTTTGTTCCGAAGCGCTAGTTTCGTCATATTCTTCAACATTTCTCTGCTTACAGCAGGACTAGCTGCAGCTCTGTTGCTATGGCCTACAGTAACCGAGCAACTTCATCACTCAAGAAAGGTTCATCGCAATCTCCAAACAAAATGCCCAACTGCTAAGACCGGTCTCCTTGGTGGTGGTGCTTTCGTGTCCCTTGACGCATCGCTCTTTTGGTTGGTGGCCCTGATGTTAGCTGACAATGCACGAGAGGACTATTTTGAGGAGACTGAAAAGGATATCAAGGGTGAATATGGACAGGTTTCTGCAATCGATTTCGATGGACATGCCAATATTAAAGGAAGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

202

Amino Acids

21.66

Weight (kDa)

8.49

Isoelectric Point (pI)

32.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 59 - 154 1e-18 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 98
AclWI GGATC 1 cut(s) 143
AcuI CTGAAG 1 cut(s) 129
AfeI AGCGCT 1 cut(s) 274
AfiI CCNNNNNNNGG 3 cut(s) 98, 113, 236
AgeI ACCGGT 1 cut(s) 419
AgsI TTSAA 1 cut(s) 295
AjnI CCWGG 1 cut(s) 189
AluBI AGCT 5 cut(s) 98, 257, 323, 329, 491
AluI AGCT 5 cut(s) 98, 257, 323, 329, 491
Alw26I GTCTC 3 cut(s) 30, 428, 515
AlwI GGATC 1 cut(s) 143
AlwNI CAGNNNCTG 2 cut(s) 98, 206
Aor51HI AGCGCT 1 cut(s) 274
AoxI GGCC 2 cut(s) 341, 477
ApeKI GCWGC 4 cut(s) 95, 257, 323, 326
AsiGI ACCGGT 1 cut(s) 419
AspLEI GCGC 1 cut(s) 275
AspS9I GGNCC 1 cut(s) 478
AsuHPI GGTGA 1 cut(s) 554
BauI CACGAG 1 cut(s) 501
BbvI GCAGC 4 cut(s) 107, 244, 310, 338
BccI CCATC 2 cut(s) 160, 569
BcgI CGANNNNNNTGC 2 cut(s) 563, 597
BciT130I CCWGG 1 cut(s) 191
BciVI GTATCC 1 cut(s) 150
BcoDI GTCTC 3 cut(s) 30, 428, 515
BfaI CTAG 2 cut(s) 276, 320
BfmI CTRYAG 3 cut(s) 198, 324, 345
BfoI RGCGCY 1 cut(s) 276
BfuI GTATCC 1 cut(s) 150
BisI GCNGC 4 cut(s) 96, 258, 324, 327
BlsI GCNGC 4 cut(s) 97, 259, 325, 328
Bme1390I CCNGG 1 cut(s) 191
BmgT120I GGNCC 1 cut(s) 478
BmrFI CCNGG 1 cut(s) 191
BmsI GCATC 1 cut(s) 467
BpuEI CTTGAG 1 cut(s) 355
Bsa29I ATCGAT 1 cut(s) 567
BsaI GGTCTC 1 cut(s) 428
BsaJI CCNNGG 1 cut(s) 427
BsaWI WCCGGW 1 cut(s) 419
Bsc4I CCNNNNNNNGG 3 cut(s) 98, 113, 236
Bse118I RCCGGY 1 cut(s) 419
BseBI CCWGG 1 cut(s) 191
BseCI ATCGAT 1 cut(s) 567
BseDI CCNNGG 1 cut(s) 427
BseLI CCNNNNNNNGG 3 cut(s) 98, 113, 236
BseRI GAGGAG 1 cut(s) 533
BseXI GCAGC 4 cut(s) 107, 244, 310, 338
BseYI CCCAGC 1 cut(s) 206
BshFI GGCC 2 cut(s) 343, 479
BshTI ACCGGT 1 cut(s) 419
BshVI ATCGAT 1 cut(s) 567
BsiSI CCGG 1 cut(s) 420
BslFI GGGAC 2 cut(s) 229, 433
BslI CCNNNNNNNGG 3 cut(s) 98, 113, 236
BsmAI GTCTC 3 cut(s) 30, 428, 515
BsmFI GGGAC 2 cut(s) 229, 433
BsnI GGCC 2 cut(s) 343, 479
Bso31I GGTCTC 1 cut(s) 428
Bsp143I GATC 2 cut(s) 129, 148
BspANI GGCC 2 cut(s) 343, 479
BspDI ATCGAT 1 cut(s) 567
BspMAI CTGCAG 1 cut(s) 328
BspPI GGATC 1 cut(s) 143
BspTNI GGTCTC 1 cut(s) 428
BsrFI RCCGGY 1 cut(s) 419
BssAI RCCGGY 1 cut(s) 419
BssECI CCNNGG 1 cut(s) 427
BssMI GATC 2 cut(s) 129, 148
BssSI CACGAG 1 cut(s) 501
BssT1I CCWWGG 1 cut(s) 427
Bst2BI CACGAG 1 cut(s) 501
Bst2UI CCWGG 1 cut(s) 191
Bst4CI ACNGT 4 cut(s) 13, 34, 202, 349
BstC8I GCNNGC 1 cut(s) 93
BstDEI CTNAG 1 cut(s) 414
BstH2I RGCGCY 1 cut(s) 276
BstHHI GCGC 1 cut(s) 275
BstKTI GATC 2 cut(s) 132, 151
BstMAI GTCTC 3 cut(s) 30, 428, 515
BstMBI GATC 2 cut(s) 129, 148
BstMWI GCNNNNNNNGC 2 cut(s) 320, 497
BstNI CCWGG 1 cut(s) 191
BstNSI RCATGY 1 cut(s) 584
BstSCI CCNGG 1 cut(s) 189
BstSFI CTRYAG 3 cut(s) 198, 324, 345
BstV1I GCAGC 4 cut(s) 107, 244, 310, 338
BstX2I RGATCY 1 cut(s) 148
BstYI RGATCY 1 cut(s) 148
Bsu15I ATCGAT 1 cut(s) 567
BsuI GTATCC 1 cut(s) 150
BsuRI GGCC 2 cut(s) 343, 479
BsuTUI ATCGAT 1 cut(s) 567
BtgZI GCGATG 2 cut(s) 368, 444
BtsIMutI CAGTG 1 cut(s) 39
Cac8I GCNNGC 1 cut(s) 93
CaiI CAGNNNCTG 2 cut(s) 98, 206
CfoI GCGC 1 cut(s) 275
Cfr10I RCCGGY 1 cut(s) 419
Cfr13I GGNCC 1 cut(s) 478
ClaI ATCGAT 1 cut(s) 567
CseI GACGC 1 cut(s) 464
CspAI ACCGGT 1 cut(s) 419
CviAII CATG 1 cut(s) 581
CviJI RGCY 8 cut(s) 91, 98, 257, 323, 329, 343, 479, 491
CviKI_1 RGCY 8 cut(s) 91, 98, 257, 323, 329, 343, 479, 491
DdeI CTNAG 1 cut(s) 414
DpnI GATC 2 cut(s) 131, 150
DpnII GATC 2 cut(s) 129, 148
Eco130I CCWWGG 1 cut(s) 427
Eco31I GGTCTC 1 cut(s) 428
Eco32I GATATC 1 cut(s) 535
Eco47III AGCGCT 1 cut(s) 274
Eco57I CTGAAG 1 cut(s) 129
EcoRII CCWGG 1 cut(s) 189
EcoRV GATATC 1 cut(s) 535
EcoT14I CCWWGG 1 cut(s) 427
ErhI CCWWGG 1 cut(s) 427
FaeI CATG 1 cut(s) 584
FaiI YATR 7 cut(s) 62, 110, 232, 287, 340, 549, 582
FaqI GGGAC 2 cut(s) 229, 433
FatI CATG 1 cut(s) 580
Fnu4HI GCNGC 4 cut(s) 96, 258, 324, 327
Fsp4HI GCNGC 4 cut(s) 96, 258, 324, 327
FspBI CTAG 2 cut(s) 276, 320
GlaI GCGC 1 cut(s) 274
GluI GCNGC 4 cut(s) 96, 258, 324, 327
GsaI CCCAGC 1 cut(s) 210
HaeII RGCGCY 1 cut(s) 276
HaeIII GGCC 2 cut(s) 343, 479
HapII CCGG 1 cut(s) 420
HgaI GACGC 1 cut(s) 464
HhaI GCGC 1 cut(s) 275
Hin1II CATG 1 cut(s) 584
Hin6I GCGC 1 cut(s) 273
HinP1I GCGC 1 cut(s) 273
HpaII CCGG 1 cut(s) 420
HphI GGTGA 1 cut(s) 554
Hpy188I TCNGA 4 cut(s) 30, 68, 148, 269
Hpy188III TCNNGA 1 cut(s) 372
HpyAV CCTTC 2 cut(s) 153, 257
HpyCH4III ACNGT 4 cut(s) 13, 34, 202, 349
HpyCH4V TGCA 4 cut(s) 135, 326, 500, 563
HpyF10VI GCNNNNNNNGC 2 cut(s) 320, 497
HpyF3I CTNAG 1 cut(s) 414
Hsp92II CATG 1 cut(s) 584
HspAI GCGC 1 cut(s) 273
Kzo9I GATC 2 cut(s) 129, 148
LpnPI CCDG 9 cut(s) 84, 105, 176, 192, 203, 300, 433, 494, 539
Lsp1109I GCAGC 4 cut(s) 107, 244, 310, 338
LweI GCATC 1 cut(s) 467
MaeI CTAG 2 cut(s) 276, 320
MaeIII GTNAC 2 cut(s) 7, 349
MalI GATC 2 cut(s) 131, 150
MboI GATC 2 cut(s) 129, 148
MboII GAAGA 1 cut(s) 283
MflI RGATCY 1 cut(s) 148
MnlI CCTC 3 cut(s) 197, 499, 511
MseI TTAA 1 cut(s) 591
MspA1I CMGCKG 1 cut(s) 98
MspI CCGG 1 cut(s) 420
MspR9I CCNGG 1 cut(s) 191
MvaI CCWGG 1 cut(s) 191
MwoI GCNNNNNNNGC 2 cut(s) 320, 497
NdeII GATC 2 cut(s) 129, 148
NlaIII CATG 1 cut(s) 584
NmuCI GTSAC 1 cut(s) 7
NspI RCATGY 1 cut(s) 584
PflMI CCANNNNNTGG 1 cut(s) 98
PinAI ACCGGT 1 cut(s) 419
PkrI GCNGC 4 cut(s) 97, 259, 325, 328
Psp6I CCWGG 1 cut(s) 189
PspFI CCCAGC 1 cut(s) 206
PspGI CCWGG 1 cut(s) 189
PspPI GGNCC 1 cut(s) 478
PstI CTGCAG 1 cut(s) 328
PstNI CAGNNNCTG 2 cut(s) 98, 206
PsuI RGATCY 1 cut(s) 148
PvuII CAGCTG 1 cut(s) 98
SaqAI TTAA 1 cut(s) 591
SatI GCNGC 4 cut(s) 96, 258, 324, 327
Sau3AI GATC 2 cut(s) 129, 148
Sau96I GGNCC 1 cut(s) 478
ScrFI CCNGG 1 cut(s) 191
SetI ASST 7 cut(s) 100, 259, 325, 331, 381, 493, 558
SfaNI GCATC 1 cut(s) 467
SfcI CTRYAG 3 cut(s) 198, 324, 345
SmlI CTYRAG 1 cut(s) 370
SmoI CTYRAG 1 cut(s) 370
SspI AATATT 1 cut(s) 589
SspMI CTAG 2 cut(s) 276, 320
StyD4I CCNGG 1 cut(s) 189
StyI CCWWGG 1 cut(s) 427
TaaI ACNGT 4 cut(s) 13, 34, 202, 349
TaqI TCGA 2 cut(s) 567, 573
Tru1I TTAA 1 cut(s) 591
Tru9I TTAA 1 cut(s) 591
TscAI CASTG 1 cut(s) 39
TseFI GTSAC 1 cut(s) 7
TseI GCWGC 4 cut(s) 95, 257, 323, 326
Tsp45I GTSAC 1 cut(s) 7
TspDTI ATGAA 3 cut(s) 49, 353, 371
TspRI CASTG 1 cut(s) 39
Van91I CCANNNNNTGG 1 cut(s) 98
XceI RCATGY 1 cut(s) 584
XspI CTAG 2 cut(s) 276, 320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.