Rh6AG512900

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0002236
Physical Location & Seq
Reverse (-)
68288099 .. 68289458
1360 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG512900.1

Sequence Viewer

Length: 627 bp
ATGGCAGTCACTATGAAGATGATGTCTGTGCTTGTTGCAGCACTGGGTATTCTTTCGTTTGTGTTTGGTATTCTTGCTGAAACTAAGAAGCCTGCAGCTGGAGTTCCCATAATAAGGAAAGGCATGGTGATTTGCAAGTACCCATCTGATCCAACGCTTGCTTATGCGTGGCTATCCTTTGGATTTCTTCTGCTATGTTCTGCGGTTGGTTGGTTCTCCATATTTTACAGCTACAAAGGCAGAAAAGCTCCCGCATCTGTTTTCTTCCGGTGCTCTACCTTCTTCTCCTTCTTCACCATTACCCTGGGTACAATAGGATTAGCAGCTGCATTTTTGCTGTGGCCTATAGCTACACATCATCACCCAAATTATAACATTATTGGGAATCAAGATCCCCACAGCGCCACATGCCCAACCGCCAAAACTGGTGTTCTCGGCGGAGGAGCCTTTATGTCCCTCAACTCATCGCTACTGTGGCTAGTTTGCTTCATCTTGGCTAGCAATGCAAGAGAAGACTACTTTGAAGAATTGGATAGTGTCCAAAAACCTAAACACATTGAGAAAGCTGAAGCTGGACATACCAAGGCACTTGAAGACGATGACAGCAGCGAGATTAGATCCCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

208

Amino Acids

22.72

Weight (kDa)

8.26

Isoelectric Point (pI)

47.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 60 - 156 3.1e-09 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 372
AciI CCGC 4 cut(s) 203, 252, 417, 438
AclWI GGATC 3 cut(s) 143, 386, 612
AcuI CTGAAG 1 cut(s) 588
AfaI GTAC 2 cut(s) 140, 310
AfiI CCNNNNNNNGG 2 cut(s) 98, 114
AgsI TTSAA 2 cut(s) 524, 593
AjnI CCWGG 1 cut(s) 303
AluBI AGCT 7 cut(s) 98, 231, 248, 326, 350, 566, 572
AluI AGCT 7 cut(s) 98, 231, 248, 326, 350, 566, 572
Alw21I GWGCWC 1 cut(s) 275
AlwI GGATC 3 cut(s) 143, 386, 612
AoxI GGCC 1 cut(s) 341
ApeKI GCWGC 5 cut(s) 38, 95, 323, 326, 606
AspLEI GCGC 1 cut(s) 404
AsuHPI GGTGA 3 cut(s) 139, 286, 353
AsuNHI GCTAGC 1 cut(s) 497
BbsI GAAGAC 2 cut(s) 519, 600
Bbv12I GWGCWC 1 cut(s) 275
BbvI GCAGC 5 cut(s) 50, 107, 313, 335, 618
BccI CCATC 1 cut(s) 151
BciT130I CCWGG 1 cut(s) 305
BfaI CTAG 2 cut(s) 479, 498
BfmI CTRYAG 2 cut(s) 93, 345
BfoI RGCGCY 1 cut(s) 405
BisI GCNGC 5 cut(s) 39, 96, 324, 327, 607
BlsI GCNGC 5 cut(s) 40, 97, 325, 328, 608
Bme1390I CCNGG 1 cut(s) 305
BmiI GGNNCC 1 cut(s) 445
BmrFI CCNGG 1 cut(s) 305
BmrI ACTGGG 2 cut(s) 53, 616
BmsI GCATC 1 cut(s) 263
BmtI GCTAGC 1 cut(s) 501
BmuI ACTGGG 2 cut(s) 53, 616
BpiI GAAGAC 2 cut(s) 519, 600
BpmI CTGGAG 1 cut(s) 120
BsaJI CCNNGG 3 cut(s) 303, 304, 582
BsaWI WCCGGW 1 cut(s) 267
Bsc4I CCNNNNNNNGG 2 cut(s) 98, 114
Bse1I ACTGG 3 cut(s) 48, 430, 622
Bse3DI GCAATG 1 cut(s) 508
BseBI CCWGG 1 cut(s) 305
BseDI CCNNGG 3 cut(s) 303, 304, 582
BseLI CCNNNNNNNGG 2 cut(s) 98, 114
BseMI GCAATG 1 cut(s) 508
BseNI ACTGG 3 cut(s) 48, 430, 622
BseRI GAGGAG 1 cut(s) 456
BseXI GCAGC 5 cut(s) 50, 107, 313, 335, 618
BshFI GGCC 1 cut(s) 343
BsiHKAI GWGCWC 1 cut(s) 275
BsiSI CCGG 1 cut(s) 268
BslFI GGGAC 1 cut(s) 439
BslI CCNNNNNNNGG 2 cut(s) 98, 114
BsmFI GGGAC 1 cut(s) 439
BsnI GGCC 1 cut(s) 343
Bsp1286I GDGCHC 1 cut(s) 275
Bsp143I GATC 3 cut(s) 148, 391, 617
BspACI CCGC 4 cut(s) 203, 252, 417, 438
BspANI GGCC 1 cut(s) 343
BspLI GGNNCC 1 cut(s) 445
BspMAI CTGCAG 1 cut(s) 97
BspOI GCTAGC 1 cut(s) 501
BspPI GGATC 3 cut(s) 143, 386, 612
BsrDI GCAATG 1 cut(s) 508
BsrI ACTGG 3 cut(s) 48, 430, 622
BssECI CCNNGG 3 cut(s) 303, 304, 582
BssMI GATC 3 cut(s) 148, 391, 617
BssT1I CCWWGG 1 cut(s) 582
Bst2UI CCWGG 1 cut(s) 305
Bst4CI ACNGT 1 cut(s) 474
BstC8I GCNNGC 3 cut(s) 93, 159, 499
BstDEI CTNAG 1 cut(s) 84
BstH2I RGCGCY 1 cut(s) 405
BstHHI GCGC 1 cut(s) 404
BstKTI GATC 3 cut(s) 151, 394, 620
BstMBI GATC 3 cut(s) 148, 391, 617
BstMWI GCNNNNNNNGC 4 cut(s) 237, 408, 475, 503
BstNI CCWGG 1 cut(s) 305
BstNSI RCATGY 1 cut(s) 411
BstSCI CCNGG 1 cut(s) 303
BstSFI CTRYAG 2 cut(s) 93, 345
BstV1I GCAGC 5 cut(s) 50, 107, 313, 335, 618
BstV2I GAAGAC 2 cut(s) 519, 600
BstX2I RGATCY 2 cut(s) 391, 617
BstXI CCANNNNNNTGG 1 cut(s) 304
BstYI RGATCY 2 cut(s) 391, 617
BsuRI GGCC 1 cut(s) 343
BtgZI GCGATG 1 cut(s) 450
BtsIMutI CAGTG 1 cut(s) 41
Cac8I GCNNGC 3 cut(s) 93, 159, 499
CfoI GCGC 1 cut(s) 404
Csp6I GTAC 2 cut(s) 139, 309
CviAII CATG 2 cut(s) 124, 408
CviQI GTAC 2 cut(s) 139, 309
DdeI CTNAG 1 cut(s) 84
DpnI GATC 3 cut(s) 150, 393, 619
DpnII GATC 3 cut(s) 148, 391, 617
EciI GGCGGA 1 cut(s) 453
Eco130I CCWWGG 1 cut(s) 582
Eco57I CTGAAG 1 cut(s) 588
EcoRII CCWGG 1 cut(s) 303
EcoT14I CCWWGG 1 cut(s) 582
ErhI CCWWGG 1 cut(s) 582
FaeI CATG 2 cut(s) 127, 411
FaqI GGGAC 1 cut(s) 439
FatI CATG 2 cut(s) 123, 407
FauI CCCGC 1 cut(s) 259
Fnu4HI GCNGC 5 cut(s) 39, 96, 324, 327, 607
Fsp4HI GCNGC 5 cut(s) 39, 96, 324, 327, 607
FspBI CTAG 2 cut(s) 479, 498
GlaI GCGC 1 cut(s) 403
GluI GCNGC 5 cut(s) 39, 96, 324, 327, 607
GsuI CTGGAG 1 cut(s) 120
HaeII RGCGCY 1 cut(s) 405
HaeIII GGCC 1 cut(s) 343
HapII CCGG 1 cut(s) 268
HhaI GCGC 1 cut(s) 404
Hin1II CATG 2 cut(s) 127, 411
Hin6I GCGC 1 cut(s) 402
HinP1I GCGC 1 cut(s) 402
HinfI GANTC 1 cut(s) 385
HpaII CCGG 1 cut(s) 268
HphI GGTGA 3 cut(s) 139, 286, 353
Hpy188I TCNGA 1 cut(s) 148
Hpy188III TCNNGA 1 cut(s) 389
HpyAV CCTTC 2 cut(s) 289, 298
HpyCH4III ACNGT 1 cut(s) 474
HpyCH4V TGCA 5 cut(s) 38, 95, 135, 329, 506
HpyF10VI GCNNNNNNNGC 4 cut(s) 237, 408, 475, 503
HpyF3I CTNAG 1 cut(s) 84
Hsp92II CATG 2 cut(s) 127, 411
HspAI GCGC 1 cut(s) 402
Kzo9I GATC 3 cut(s) 148, 391, 617
LmnI GCTCC 2 cut(s) 253, 443
LpnPI CCDG 8 cut(s) 29, 84, 105, 281, 290, 317, 411, 558
Lsp1109I GCAGC 5 cut(s) 50, 107, 313, 335, 618
LweI GCATC 1 cut(s) 263
MaeI CTAG 2 cut(s) 479, 498
MaeIII GTNAC 1 cut(s) 7
MalI GATC 3 cut(s) 150, 393, 619
MboI GATC 3 cut(s) 148, 391, 617
MboII GAAGA 8 cut(s) 28, 179, 256, 274, 283, 524, 536, 605
MflI RGATCY 2 cut(s) 391, 617
MhlI GDGCHC 1 cut(s) 275
MluCI AATT 2 cut(s) 367, 527
MmeI TCCRAC 1 cut(s) 176
MnlI CCTC 2 cut(s) 434, 467
MspA1I CMGCKG 2 cut(s) 98, 326
MspI CCGG 1 cut(s) 268
MspR9I CCNGG 1 cut(s) 305
MvaI CCWGG 1 cut(s) 305
MwoI GCNNNNNNNGC 4 cut(s) 237, 408, 475, 503
NdeII GATC 3 cut(s) 148, 391, 617
NheI GCTAGC 1 cut(s) 497
NlaIII CATG 2 cut(s) 127, 411
NlaIV GGNNCC 1 cut(s) 445
NmeAIII GCCGAG 1 cut(s) 414
NmuCI GTSAC 1 cut(s) 7
NspI RCATGY 1 cut(s) 411
PasI CCCWGGG 1 cut(s) 304
PfeI GAWTC 1 cut(s) 385
PkrI GCNGC 5 cut(s) 40, 97, 325, 328, 608
PsiI TTATAA 1 cut(s) 372
Psp6I CCWGG 1 cut(s) 303
PspGI CCWGG 1 cut(s) 303
PspN4I GGNNCC 1 cut(s) 445
PstI CTGCAG 1 cut(s) 97
PsuI RGATCY 2 cut(s) 391, 617
PvuII CAGCTG 2 cut(s) 98, 326
RsaI GTAC 2 cut(s) 140, 310
RsaNI GTAC 2 cut(s) 139, 309
SatI GCNGC 5 cut(s) 39, 96, 324, 327, 607
Sau3AI GATC 3 cut(s) 148, 391, 617
ScrFI CCNGG 1 cut(s) 305
SduI GDGCHC 1 cut(s) 275
SetI ASST 9 cut(s) 100, 233, 250, 281, 328, 352, 550, 568, 574
SfaNI GCATC 1 cut(s) 263
SfcI CTRYAG 2 cut(s) 93, 345
Sse9I AATT 2 cut(s) 367, 527
SsiI CCGC 4 cut(s) 203, 252, 417, 438
SspMI CTAG 2 cut(s) 479, 498
StyD4I CCNGG 1 cut(s) 303
StyI CCWWGG 1 cut(s) 582
TaaI ACNGT 1 cut(s) 474
TasI AATT 2 cut(s) 367, 527
TfiI GAWTC 1 cut(s) 385
TscAI CASTG 1 cut(s) 48
TseFI GTSAC 1 cut(s) 7
TseI GCWGC 5 cut(s) 38, 95, 323, 326, 606
Tsp45I GTSAC 1 cut(s) 7
TspDTI ATGAA 2 cut(s) 29, 478
TspRI CASTG 1 cut(s) 48
XceI RCATGY 1 cut(s) 411
XspI CTAG 2 cut(s) 479, 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.