RchiOBHm_Chr5g0063261

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0002236
Physical Location & Seq
Reverse (-)
69022162 .. 69023226
1065 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33937

Sequence Viewer

Length: 504 bp
ATGGTGATTTGCAAATACCCATCTGATCCAACGCTTGCTTATGCGTGGCTATCTTTCGGATTTCTTCTGTTATGTTCTGCGGTTGGTTGGTTCTCTCTATTTTACAGCTACAAAGGCAGAAAAGCTCCCACATCTGTTTTTTTCCGGAGCTCTACCTTCTTCTCCTTCTTCACCATTACCCTGGGTACAATAGGATTAGCAGCTGCATTTTTGCTATGGCCTATAGCGACGCATCATCACCCAAATTATAACATTACTGGGAATCAAGACCCCCACAGCGCCACATGCCCAACTGCCAAAACTGGTGTTCTTGGCGGAGGAGCCTTCTTGTCCCTCAACTCATCGCTACTGTGGTTAGTTTGCTTCATCTTGGCTAGCAATGCAAGAGAAGACTACTTTGAAGACTTGGATAGTGTCGAAAAACCTAAACACATTGAGAAAGCTGAAGCTGGACATACCACGGCACTTGAAGATGATTACAGCAGCGAGATTATCTCCCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

167

Amino Acids

18.42

Weight (kDa)

5.79

Isoelectric Point (pI)

44.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 19 - 115 8.6e-11 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 249
AccIII TCCGGA 1 cut(s) 144
AciI CCGC 2 cut(s) 80, 315
AclWI GGATC 1 cut(s) 20
AcuI CTGAAG 1 cut(s) 465
AfaI GTAC 1 cut(s) 187
AgsI TTSAA 2 cut(s) 401, 470
AjnI CCWGG 1 cut(s) 180
AluBI AGCT 6 cut(s) 108, 125, 150, 203, 443, 449
AluI AGCT 6 cut(s) 108, 125, 150, 203, 443, 449
Alw21I GWGCWC 1 cut(s) 152
AlwI GGATC 1 cut(s) 20
Aor13HI TCCGGA 1 cut(s) 144
AoxI GGCC 1 cut(s) 218
ApeKI GCWGC 3 cut(s) 200, 203, 483
AspLEI GCGC 1 cut(s) 281
AsuHPI GGTGA 3 cut(s) 16, 163, 230
AsuNHI GCTAGC 1 cut(s) 374
BanII GRGCYC 1 cut(s) 152
BbsI GAAGAC 2 cut(s) 396, 408
Bbv12I GWGCWC 1 cut(s) 152
BbvI GCAGC 3 cut(s) 190, 212, 495
BccI CCATC 1 cut(s) 28
BceAI ACGGC 1 cut(s) 477
BciT130I CCWGG 1 cut(s) 182
BfaI CTAG 1 cut(s) 375
BfmI CTRYAG 1 cut(s) 222
BfoI RGCGCY 1 cut(s) 282
BisI GCNGC 3 cut(s) 201, 204, 484
BlsI GCNGC 3 cut(s) 202, 205, 485
Bme1390I CCNGG 1 cut(s) 182
BmiI GGNNCC 1 cut(s) 322
BmrFI CCNGG 1 cut(s) 182
BmrI ACTGGG 2 cut(s) 267, 493
BmsI GCATC 1 cut(s) 241
BmtI GCTAGC 1 cut(s) 378
BmuI ACTGGG 2 cut(s) 267, 493
BpiI GAAGAC 2 cut(s) 396, 408
BplI GAGNNNNNCTC 1 cut(s) 479
BsaJI CCNNGG 3 cut(s) 180, 181, 459
BsaWI WCCGGW 1 cut(s) 144
Bse1I ACTGG 3 cut(s) 262, 307, 499
Bse3DI GCAATG 1 cut(s) 385
BseAI TCCGGA 1 cut(s) 144
BseBI CCWGG 1 cut(s) 182
BseDI CCNNGG 3 cut(s) 180, 181, 459
BseMI GCAATG 1 cut(s) 385
BseNI ACTGG 3 cut(s) 262, 307, 499
BseRI GAGGAG 1 cut(s) 333
BseXI GCAGC 3 cut(s) 190, 212, 495
BshFI GGCC 1 cut(s) 220
BsiHKAI GWGCWC 1 cut(s) 152
BsiSI CCGG 1 cut(s) 145
BslFI GGGAC 1 cut(s) 316
BsmFI GGGAC 1 cut(s) 316
BsnI GGCC 1 cut(s) 220
Bsp1286I GDGCHC 1 cut(s) 152
Bsp13I TCCGGA 1 cut(s) 144
Bsp143I GATC 1 cut(s) 25
BspACI CCGC 2 cut(s) 80, 315
BspANI GGCC 1 cut(s) 220
BspEI TCCGGA 1 cut(s) 144
BspLI GGNNCC 1 cut(s) 322
BspOI GCTAGC 1 cut(s) 378
BspPI GGATC 1 cut(s) 20
BsrDI GCAATG 1 cut(s) 385
BsrI ACTGG 3 cut(s) 262, 307, 499
BssECI CCNNGG 3 cut(s) 180, 181, 459
BssMI GATC 1 cut(s) 25
Bst2UI CCWGG 1 cut(s) 182
Bst4CI ACNGT 1 cut(s) 351
BstC8I GCNNGC 2 cut(s) 36, 376
BstDSI CCRYGG 1 cut(s) 459
BstH2I RGCGCY 1 cut(s) 282
BstHHI GCGC 1 cut(s) 281
BstKTI GATC 1 cut(s) 28
BstMBI GATC 1 cut(s) 25
BstMWI GCNNNNNNNGC 3 cut(s) 114, 285, 380
BstNI CCWGG 1 cut(s) 182
BstNSI RCATGY 1 cut(s) 288
BstSCI CCNGG 1 cut(s) 180
BstSFI CTRYAG 1 cut(s) 222
BstV1I GCAGC 3 cut(s) 190, 212, 495
BstV2I GAAGAC 2 cut(s) 396, 408
BstXI CCANNNNNNTGG 1 cut(s) 181
BsuRI GGCC 1 cut(s) 220
BtgI CCRYGG 1 cut(s) 459
BtgZI GCGATG 1 cut(s) 327
Cac8I GCNNGC 2 cut(s) 36, 376
CfoI GCGC 1 cut(s) 281
CseI GACGC 1 cut(s) 238
Csp6I GTAC 1 cut(s) 186
CspCI CAANNNNNGTGG 2 cut(s) 448, 483
CviAII CATG 1 cut(s) 285
CviQI GTAC 1 cut(s) 186
DpnI GATC 1 cut(s) 27
DpnII GATC 1 cut(s) 25
EciI GGCGGA 1 cut(s) 330
Ecl136II GAGCTC 1 cut(s) 150
Eco24I GRGCYC 1 cut(s) 152
Eco53kI GAGCTC 1 cut(s) 150
Eco57I CTGAAG 1 cut(s) 465
EcoICRI GAGCTC 1 cut(s) 150
EcoRII CCWGG 1 cut(s) 180
EcoT38I GRGCYC 1 cut(s) 152
FaeI CATG 1 cut(s) 288
FaiI YATR 7 cut(s) 42, 73, 217, 224, 249, 286, 456
FaqI GGGAC 1 cut(s) 316
FatI CATG 1 cut(s) 284
Fnu4HI GCNGC 3 cut(s) 201, 204, 484
FriOI GRGCYC 1 cut(s) 152
Fsp4HI GCNGC 3 cut(s) 201, 204, 484
FspBI CTAG 1 cut(s) 375
GlaI GCGC 1 cut(s) 280
GluI GCNGC 3 cut(s) 201, 204, 484
HaeII RGCGCY 1 cut(s) 282
HaeIII GGCC 1 cut(s) 220
HapII CCGG 1 cut(s) 145
HgaI GACGC 1 cut(s) 238
HhaI GCGC 1 cut(s) 281
Hin1II CATG 1 cut(s) 288
Hin6I GCGC 1 cut(s) 279
HinP1I GCGC 1 cut(s) 279
HinfI GANTC 1 cut(s) 262
HpaII CCGG 1 cut(s) 145
HphI GGTGA 3 cut(s) 16, 163, 230
Hpy188I TCNGA 2 cut(s) 25, 59
Hpy188III TCNNGA 2 cut(s) 145, 266
Hpy99I CGWCG 1 cut(s) 232
HpyAV CCTTC 3 cut(s) 166, 175, 334
HpyCH4III ACNGT 1 cut(s) 351
HpyCH4V TGCA 3 cut(s) 12, 206, 383
HpyF10VI GCNNNNNNNGC 3 cut(s) 114, 285, 380
Hsp92II CATG 1 cut(s) 288
HspAI GCGC 1 cut(s) 279
Kpn2I TCCGGA 1 cut(s) 144
Kzo9I GATC 1 cut(s) 25
LmnI GCTCC 3 cut(s) 130, 147, 320
LpnPI CCDG 6 cut(s) 158, 167, 194, 243, 288, 435
Lsp1109I GCAGC 3 cut(s) 190, 212, 495
LweI GCATC 1 cut(s) 241
MaeI CTAG 1 cut(s) 375
MalI GATC 1 cut(s) 27
MboI GATC 1 cut(s) 25
MboII GAAGA 6 cut(s) 56, 151, 160, 401, 413, 482
MhlI GDGCHC 1 cut(s) 152
MluCI AATT 1 cut(s) 244
MmeI TCCRAC 1 cut(s) 53
MnlI CCTC 2 cut(s) 311, 344
MroI TCCGGA 1 cut(s) 144
MspA1I CMGCKG 1 cut(s) 203
MspI CCGG 1 cut(s) 145
MspR9I CCNGG 1 cut(s) 182
MvaI CCWGG 1 cut(s) 182
MwoI GCNNNNNNNGC 3 cut(s) 114, 285, 380
NdeII GATC 1 cut(s) 25
NheI GCTAGC 1 cut(s) 374
NlaIII CATG 1 cut(s) 288
NlaIV GGNNCC 1 cut(s) 322
NspI RCATGY 1 cut(s) 288
PasI CCCWGGG 1 cut(s) 181
PfeI GAWTC 1 cut(s) 262
PkrI GCNGC 3 cut(s) 202, 205, 485
PsiI TTATAA 1 cut(s) 249
Psp124BI GAGCTC 1 cut(s) 152
Psp6I CCWGG 1 cut(s) 180
PspGI CCWGG 1 cut(s) 180
PspN4I GGNNCC 1 cut(s) 322
PvuII CAGCTG 1 cut(s) 203
RsaI GTAC 1 cut(s) 187
RsaNI GTAC 1 cut(s) 186
SacI GAGCTC 1 cut(s) 152
SatI GCNGC 3 cut(s) 201, 204, 484
Sau3AI GATC 1 cut(s) 25
ScrFI CCNGG 1 cut(s) 182
SduI GDGCHC 1 cut(s) 152
SetI ASST 8 cut(s) 110, 127, 152, 158, 205, 427, 445, 451
SfaNI GCATC 1 cut(s) 241
SfcI CTRYAG 1 cut(s) 222
Sse9I AATT 1 cut(s) 244
SsiI CCGC 2 cut(s) 80, 315
SspMI CTAG 1 cut(s) 375
SstI GAGCTC 1 cut(s) 152
StyD4I CCNGG 1 cut(s) 180
TaaI ACNGT 1 cut(s) 351
TaqI TCGA 1 cut(s) 417
TasI AATT 1 cut(s) 244
TfiI GAWTC 1 cut(s) 262
TseI GCWGC 3 cut(s) 200, 203, 483
TspDTI ATGAA 1 cut(s) 355
XceI RCATGY 1 cut(s) 288
XspI CTAG 1 cut(s) 375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.