MD08G1132500.v1.1

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0002236
Physical Location & Seq
Forward (+)
12521163 .. 12522981
1819 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1132500.v1.1.491

Sequence Viewer

Length: 627 bp
ATGGCAGTGACTATGATAAAGATGGGTTTGATTGTTGCGGTATTGGGTATAGTGTCGTTTGCATTCGGAATTGCTGCTGAAGTCCATAAGCCTGCAGCTGGTATCCCCATAATGAGCCGGAATGGCGTAGTGATGTGCAAGTACCCATCTGATCCAACGGTTGTATATGGGTATCTGTCCTTTGCATTTTTAGTGGCATCTTCTTTGACTGGATTCCTCTCTGTATTTTACCCATACAAGGGCAAATCAGTTCCATCCAGTGCTTTTCTCCAAAGCACTTTATTTGTTACTTTCTTCAACAGCACTCTGGGTGCAATCGGATTAGCAGCAGCATTATTGTTATGGCCAACGATTTCTGGGCAGCAGCACCACAACAACAACATGTATGAAAATCTCAGCAGCGCAAGTGGTTGCCCTACAGCCAAAACTGGTGTTCTTGGCGGAGGAGCTTTTTTATCTCTCAATTCGTCCCTACTGTGGTTGGTTTGCCTCATGCTGGCTAGCAATGCAAGATACGACTTTTTGGAAGATTTGAGCGGAGAATCTAAAGCTAAGGAAGCTACCACTTCTGGGTATAATGCAGTGCTTGAAAAAGGACTGGATAACATTTTTGACGGCGTGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

209

Amino Acids

21.96

Weight (kDa)

6.28

Isoelectric Point (pI)

35.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 60 - 157 3.8e-14 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 537
AciI CCGC 3 cut(s) 38, 441, 537
AclWI GGATC 1 cut(s) 146
AcoI YGGCCR 1 cut(s) 344
AcuI CTGAAG 1 cut(s) 99
AfaI GTAC 1 cut(s) 143
AfiI CCNNNNNNNGG 6 cut(s) 98, 238, 239, 477, 496, 570
AflIII ACRYGT 1 cut(s) 381
AgsI TTSAA 2 cut(s) 298, 590
AluBI AGCT 4 cut(s) 98, 449, 551, 560
AluI AGCT 4 cut(s) 98, 449, 551, 560
AlwI GGATC 1 cut(s) 146
AoxI GGCC 1 cut(s) 344
ApeKI GCWGC 7 cut(s) 74, 95, 326, 329, 361, 364, 399
AspLEI GCGC 1 cut(s) 404
AsuNHI GCTAGC 1 cut(s) 500
BalI TGGCCA 1 cut(s) 346
BbvI GCAGC 7 cut(s) 61, 107, 338, 341, 373, 376, 411
BccI CCATC 3 cut(s) 16, 154, 262
BciVI GTATCC 1 cut(s) 113
BfaI CTAG 1 cut(s) 501
BfmI CTRYAG 2 cut(s) 93, 417
BfuI GTATCC 1 cut(s) 113
BglI GCCNNNNNGGC 1 cut(s) 123
BisI GCNGC 7 cut(s) 75, 96, 327, 330, 362, 365, 400
BlsI GCNGC 7 cut(s) 76, 97, 328, 331, 363, 366, 401
BmsI GCATC 1 cut(s) 206
BmtI GCTAGC 1 cut(s) 504
Bpu10I CCTNAGC 1 cut(s) 552
Bsc4I CCNNNNNNNGG 6 cut(s) 98, 238, 239, 477, 496, 570
Bse1I ACTGG 4 cut(s) 214, 258, 433, 603
Bse3DI GCAATG 1 cut(s) 511
BseGI GGATG 1 cut(s) 254
BseLI CCNNNNNNNGG 6 cut(s) 98, 238, 239, 477, 496, 570
BseMI GCAATG 1 cut(s) 511
BseMII CTCAG 1 cut(s) 409
BseNI ACTGG 4 cut(s) 214, 258, 433, 603
BseRI GAGGAG 1 cut(s) 459
BseXI GCAGC 7 cut(s) 61, 107, 338, 341, 373, 376, 411
BshFI GGCC 1 cut(s) 346
BsiSI CCGG 1 cut(s) 118
BslFI GGGAC 1 cut(s) 454
BslI CCNNNNNNNGG 6 cut(s) 98, 238, 239, 477, 496, 570
BsmFI GGGAC 1 cut(s) 454
BsmI GAATGC 1 cut(s) 62
BsnI GGCC 1 cut(s) 346
Bsp143I GATC 1 cut(s) 151
BspACI CCGC 3 cut(s) 38, 441, 537
BspANI GGCC 1 cut(s) 346
BspCNI CTCAG 1 cut(s) 408
BspMAI CTGCAG 1 cut(s) 97
BspOI GCTAGC 1 cut(s) 504
BspPI GGATC 1 cut(s) 146
BsrBI CCGCTC 1 cut(s) 537
BsrDI GCAATG 1 cut(s) 511
BsrI ACTGG 4 cut(s) 214, 258, 433, 603
BssMI GATC 1 cut(s) 151
Bst4CI ACNGT 2 cut(s) 160, 477
BstC8I GCNNGC 3 cut(s) 93, 498, 502
BstDEI CTNAG 2 cut(s) 395, 552
BstF5I GGATG 1 cut(s) 254
BstHHI GCGC 1 cut(s) 404
BstKTI GATC 1 cut(s) 154
BstMBI GATC 1 cut(s) 151
BstMWI GCNNNNNNNGC 3 cut(s) 123, 506, 557
BstNSI RCATGY 1 cut(s) 385
BstSFI CTRYAG 2 cut(s) 93, 417
BstV1I GCAGC 7 cut(s) 61, 107, 338, 341, 373, 376, 411
BsuI GTATCC 1 cut(s) 113
BsuRI GGCC 1 cut(s) 346
BtsCI GGATG 1 cut(s) 254
BtsI GCAGTG 2 cut(s) 12, 588
BtsIMutI CAGTG 3 cut(s) 12, 265, 588
Cac8I GCNNGC 3 cut(s) 93, 498, 502
CfoI GCGC 1 cut(s) 404
Csp6I GTAC 1 cut(s) 142
CviAII CATG 2 cut(s) 382, 493
CviJI RGCY 9 cut(s) 91, 98, 117, 346, 422, 449, 500, 551, 560
CviKI_1 RGCY 9 cut(s) 91, 98, 117, 346, 422, 449, 500, 551, 560
CviQI GTAC 1 cut(s) 142
DdeI CTNAG 2 cut(s) 395, 552
DpnI GATC 1 cut(s) 153
DpnII GATC 1 cut(s) 151
EaeI YGGCCR 1 cut(s) 344
EciI GGCGGA 1 cut(s) 456
Eco57I CTGAAG 1 cut(s) 99
FaeI CATG 2 cut(s) 385, 496
FaqI GGGAC 1 cut(s) 454
FatI CATG 2 cut(s) 381, 492
Fnu4HI GCNGC 7 cut(s) 75, 96, 327, 330, 362, 365, 400
FokI GGATG 1 cut(s) 241
Fsp4HI GCNGC 7 cut(s) 75, 96, 327, 330, 362, 365, 400
FspBI CTAG 1 cut(s) 501
GlaI GCGC 1 cut(s) 403
GluI GCNGC 7 cut(s) 75, 96, 327, 330, 362, 365, 400
HaeIII GGCC 1 cut(s) 346
HapII CCGG 1 cut(s) 118
HhaI GCGC 1 cut(s) 404
Hin1II CATG 2 cut(s) 385, 496
Hin6I GCGC 1 cut(s) 402
HinP1I GCGC 1 cut(s) 402
HinfI GANTC 2 cut(s) 213, 542
HpaII CCGG 1 cut(s) 118
Hpy188I TCNGA 3 cut(s) 68, 151, 320
HpyCH4III ACNGT 2 cut(s) 160, 477
HpyCH4V TGCA 7 cut(s) 62, 95, 138, 185, 314, 509, 581
HpyF10VI GCNNNNNNNGC 3 cut(s) 123, 506, 557
HpyF3I CTNAG 2 cut(s) 395, 552
Hsp92II CATG 2 cut(s) 385, 496
HspAI GCGC 1 cut(s) 402
Kzo9I GATC 1 cut(s) 151
LmnI GCTCC 1 cut(s) 446
Lsp1109I GCAGC 7 cut(s) 61, 107, 338, 341, 373, 376, 411
LweI GCATC 1 cut(s) 206
MaeI CTAG 1 cut(s) 501
MaeIII GTNAC 2 cut(s) 7, 286
MalI GATC 1 cut(s) 153
MbiI CCGCTC 1 cut(s) 537
MboI GATC 1 cut(s) 151
MboII GAAGA 3 cut(s) 192, 286, 539
MlsI TGGCCA 1 cut(s) 346
MluCI AATT 2 cut(s) 69, 463
MluNI TGGCCA 1 cut(s) 346
MmeI TCCRAC 1 cut(s) 179
MnlI CCTC 3 cut(s) 227, 437, 500
Mox20I TGGCCA 1 cut(s) 346
MscI TGGCCA 1 cut(s) 346
Msp20I TGGCCA 1 cut(s) 346
MspA1I CMGCKG 1 cut(s) 98
MspI CCGG 1 cut(s) 118
Mva1269I GAATGC 1 cut(s) 62
MwoI GCNNNNNNNGC 3 cut(s) 123, 506, 557
NdeII GATC 1 cut(s) 151
NheI GCTAGC 1 cut(s) 500
NlaIII CATG 2 cut(s) 385, 496
NmuCI GTSAC 1 cut(s) 7
NspI RCATGY 1 cut(s) 385
PciI ACATGT 1 cut(s) 381
PctI GAATGC 1 cut(s) 62
PfeI GAWTC 2 cut(s) 213, 542
PkrI GCNGC 7 cut(s) 76, 97, 328, 331, 363, 366, 401
PscI ACATGT 1 cut(s) 381
PstI CTGCAG 1 cut(s) 97
PvuII CAGCTG 1 cut(s) 98
RsaI GTAC 1 cut(s) 143
RsaNI GTAC 1 cut(s) 142
SatI GCNGC 7 cut(s) 75, 96, 327, 330, 362, 365, 400
Sau3AI GATC 1 cut(s) 151
SetI ASST 4 cut(s) 100, 451, 553, 562
SfaNI GCATC 1 cut(s) 206
SfcI CTRYAG 2 cut(s) 93, 417
Sse9I AATT 2 cut(s) 69, 463
SsiI CCGC 3 cut(s) 38, 441, 537
SspMI CTAG 1 cut(s) 501
TaaI ACNGT 2 cut(s) 160, 477
TasI AATT 2 cut(s) 69, 463
TfiI GAWTC 2 cut(s) 213, 542
TscAI CASTG 3 cut(s) 12, 265, 588
TseFI GTSAC 1 cut(s) 7
TseI GCWGC 7 cut(s) 74, 95, 326, 329, 361, 364, 399
Tsp45I GTSAC 1 cut(s) 7
TspDTI ATGAA 1 cut(s) 402
TspRI CASTG 3 cut(s) 12, 265, 588
XceI RCATGY 1 cut(s) 385
XspI CTAG 1 cut(s) 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.