RchiOBHm_Chr5g0063271

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0002236
Physical Location & Seq
Reverse (-)
69026524 .. 69027795
1272 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33938

Sequence Viewer

Length: 630 bp
ATGGCAGTCACCATGAAGATTATGAGTGTGCTTGTTGCAGCACTGGGTATTCTTTCGTTTGTGTTTGGTATTCTCGCTGAAACTAAGAAGCCTGCAGCTGGAGTTCCCATAATAGGGAAAGTCATGGTGATTTGCAAGTACCCATCTGATCCAACGCTTGCTTATGCGTGGCTATCCTTTGGATTTCTTCTGTTATGTTCTGCGGTTGGTTGGTTCTCTATATTTTACAGCTACAAAGGCAGAAAAGCTCCCACATCTGTTTTCTTCCGGAGCTCTACCTTCTTCTCCTTCTTCACCATTACCCTGGGTACAATAGGATTATCAGCTGCATTTTTGCTATGGCCTATAGCGACGCATCATCACCCAAATTATAACATTACTGGGAATCAAGACCCCCACAGCGCCACATGCCCAACTGCCAAAACTGGTGTTCTTGGCGGAGGAGCCTTCTTGTCCCTCAACTCATCGCTACTGTGGTTAGTTTGGTTCATCTTGGCTAGCAATGCAAGAGAAGACTACTTTGAAGACTTGGATAGTGTCGAAAAACCTAAACACGTTGAGAAAGCTGAAGCTGGACATACCACGGCACTTGAAGATGATTACAGCAGCGAGATTATCTCCCAGTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

209

Amino Acids

22.84

Weight (kDa)

6.58

Isoelectric Point (pI)

36.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 60 - 156 1e-09 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 372
AccIII TCCGGA 1 cut(s) 267
AciI CCGC 2 cut(s) 203, 438
AclWI GGATC 1 cut(s) 143
AcuI CTGAAG 1 cut(s) 588
AfaI GTAC 3 cut(s) 140, 310, 626
AfiI CCNNNNNNNGG 3 cut(s) 98, 113, 114
AflIII ACRYGT 1 cut(s) 553
AgsI TTSAA 2 cut(s) 524, 593
AjnI CCWGG 1 cut(s) 303
AluBI AGCT 7 cut(s) 98, 231, 248, 273, 326, 566, 572
AluI AGCT 7 cut(s) 98, 231, 248, 273, 326, 566, 572
Alw21I GWGCWC 1 cut(s) 275
AlwI GGATC 1 cut(s) 143
Aor13HI TCCGGA 1 cut(s) 267
AoxI GGCC 1 cut(s) 341
ApeKI GCWGC 4 cut(s) 38, 95, 326, 606
AspLEI GCGC 1 cut(s) 404
AsuHPI GGTGA 3 cut(s) 139, 286, 353
AsuNHI GCTAGC 1 cut(s) 497
BanII GRGCYC 1 cut(s) 275
BbsI GAAGAC 2 cut(s) 519, 531
Bbv12I GWGCWC 1 cut(s) 275
BbvI GCAGC 4 cut(s) 50, 107, 313, 618
BccI CCATC 1 cut(s) 151
BceAI ACGGC 1 cut(s) 600
BciT130I CCWGG 1 cut(s) 305
BfaI CTAG 2 cut(s) 498, 628
BfmI CTRYAG 2 cut(s) 93, 345
BfoI RGCGCY 1 cut(s) 405
BisI GCNGC 4 cut(s) 39, 96, 327, 607
BlsI GCNGC 4 cut(s) 40, 97, 328, 608
BmcAI AGTACT 1 cut(s) 626
Bme1390I CCNGG 1 cut(s) 305
BmiI GGNNCC 1 cut(s) 445
BmrFI CCNGG 1 cut(s) 305
BmrI ACTGGG 3 cut(s) 53, 390, 616
BmsI GCATC 1 cut(s) 364
BmtI GCTAGC 1 cut(s) 501
BmuI ACTGGG 3 cut(s) 53, 390, 616
BpiI GAAGAC 2 cut(s) 519, 531
BplI GAGNNNNNCTC 1 cut(s) 602
BpmI CTGGAG 1 cut(s) 120
BsaJI CCNNGG 3 cut(s) 303, 304, 582
BsaWI WCCGGW 1 cut(s) 267
Bsc4I CCNNNNNNNGG 3 cut(s) 98, 113, 114
Bse1I ACTGG 4 cut(s) 48, 385, 430, 622
Bse3DI GCAATG 1 cut(s) 508
BseAI TCCGGA 1 cut(s) 267
BseBI CCWGG 1 cut(s) 305
BseDI CCNNGG 3 cut(s) 303, 304, 582
BseLI CCNNNNNNNGG 3 cut(s) 98, 113, 114
BseMI GCAATG 1 cut(s) 508
BseNI ACTGG 4 cut(s) 48, 385, 430, 622
BseRI GAGGAG 1 cut(s) 456
BseXI GCAGC 4 cut(s) 50, 107, 313, 618
BshFI GGCC 1 cut(s) 343
BsiHKAI GWGCWC 1 cut(s) 275
BsiSI CCGG 1 cut(s) 268
BslFI GGGAC 1 cut(s) 439
BslI CCNNNNNNNGG 3 cut(s) 98, 113, 114
BsmFI GGGAC 1 cut(s) 439
BsnI GGCC 1 cut(s) 343
Bsp1286I GDGCHC 1 cut(s) 275
Bsp13I TCCGGA 1 cut(s) 267
Bsp143I GATC 1 cut(s) 148
BspACI CCGC 2 cut(s) 203, 438
BspANI GGCC 1 cut(s) 343
BspEI TCCGGA 1 cut(s) 267
BspLI GGNNCC 1 cut(s) 445
BspMAI CTGCAG 1 cut(s) 97
BspOI GCTAGC 1 cut(s) 501
BspPI GGATC 1 cut(s) 143
BsrDI GCAATG 1 cut(s) 508
BsrI ACTGG 4 cut(s) 48, 385, 430, 622
BssECI CCNNGG 3 cut(s) 303, 304, 582
BssMI GATC 1 cut(s) 148
Bst2UI CCWGG 1 cut(s) 305
Bst4CI ACNGT 1 cut(s) 474
BstC8I GCNNGC 3 cut(s) 93, 159, 499
BstDEI CTNAG 1 cut(s) 84
BstDSI CCRYGG 1 cut(s) 582
BstH2I RGCGCY 1 cut(s) 405
BstHHI GCGC 1 cut(s) 404
BstKTI GATC 1 cut(s) 151
BstMBI GATC 1 cut(s) 148
BstMWI GCNNNNNNNGC 3 cut(s) 237, 408, 503
BstNI CCWGG 1 cut(s) 305
BstNSI RCATGY 1 cut(s) 411
BstSCI CCNGG 1 cut(s) 303
BstSFI CTRYAG 2 cut(s) 93, 345
BstV1I GCAGC 4 cut(s) 50, 107, 313, 618
BstV2I GAAGAC 2 cut(s) 519, 531
BstXI CCANNNNNNTGG 1 cut(s) 304
BsuRI GGCC 1 cut(s) 343
BtgI CCRYGG 1 cut(s) 582
BtgZI GCGATG 1 cut(s) 450
BtsIMutI CAGTG 1 cut(s) 41
Cac8I GCNNGC 3 cut(s) 93, 159, 499
CfoI GCGC 1 cut(s) 404
CseI GACGC 1 cut(s) 361
Csp6I GTAC 3 cut(s) 139, 309, 625
CspCI CAANNNNNGTGG 2 cut(s) 571, 606
CviAII CATG 3 cut(s) 13, 124, 408
CviQI GTAC 3 cut(s) 139, 309, 625
DdeI CTNAG 1 cut(s) 84
DpnI GATC 1 cut(s) 150
DpnII GATC 1 cut(s) 148
EciI GGCGGA 1 cut(s) 453
Ecl136II GAGCTC 1 cut(s) 273
Eco24I GRGCYC 1 cut(s) 275
Eco53kI GAGCTC 1 cut(s) 273
Eco57I CTGAAG 1 cut(s) 588
EcoICRI GAGCTC 1 cut(s) 273
EcoRII CCWGG 1 cut(s) 303
EcoT38I GRGCYC 1 cut(s) 275
FaeI CATG 3 cut(s) 16, 127, 411
FaqI GGGAC 1 cut(s) 439
FatI CATG 3 cut(s) 12, 123, 407
Fnu4HI GCNGC 4 cut(s) 39, 96, 327, 607
FriOI GRGCYC 1 cut(s) 275
Fsp4HI GCNGC 4 cut(s) 39, 96, 327, 607
FspBI CTAG 2 cut(s) 498, 628
GlaI GCGC 1 cut(s) 403
GluI GCNGC 4 cut(s) 39, 96, 327, 607
GsuI CTGGAG 1 cut(s) 120
HaeII RGCGCY 1 cut(s) 405
HaeIII GGCC 1 cut(s) 343
HapII CCGG 1 cut(s) 268
HgaI GACGC 1 cut(s) 361
HhaI GCGC 1 cut(s) 404
Hin1II CATG 3 cut(s) 16, 127, 411
Hin6I GCGC 1 cut(s) 402
HinP1I GCGC 1 cut(s) 402
HinfI GANTC 1 cut(s) 385
HpaII CCGG 1 cut(s) 268
HphI GGTGA 3 cut(s) 139, 286, 353
Hpy188I TCNGA 1 cut(s) 148
Hpy188III TCNNGA 2 cut(s) 268, 389
Hpy99I CGWCG 1 cut(s) 355
HpyAV CCTTC 3 cut(s) 289, 298, 457
HpyCH4III ACNGT 1 cut(s) 474
HpyCH4IV ACGT 1 cut(s) 555
HpyCH4V TGCA 5 cut(s) 38, 95, 135, 329, 506
HpyF10VI GCNNNNNNNGC 3 cut(s) 237, 408, 503
HpyF3I CTNAG 1 cut(s) 84
HpySE526I ACGT 1 cut(s) 555
Hsp92II CATG 3 cut(s) 16, 127, 411
HspAI GCGC 1 cut(s) 402
Kpn2I TCCGGA 1 cut(s) 267
Kzo9I GATC 1 cut(s) 148
LmnI GCTCC 3 cut(s) 253, 270, 443
LpnPI CCDG 9 cut(s) 29, 84, 105, 281, 290, 317, 366, 411, 558
Lsp1109I GCAGC 4 cut(s) 50, 107, 313, 618
LweI GCATC 1 cut(s) 364
MaeI CTAG 2 cut(s) 498, 628
MaeII ACGT 1 cut(s) 555
MaeIII GTNAC 1 cut(s) 7
MalI GATC 1 cut(s) 150
MboI GATC 1 cut(s) 148
MboII GAAGA 8 cut(s) 28, 179, 256, 274, 283, 524, 536, 605
MhlI GDGCHC 1 cut(s) 275
MluCI AATT 1 cut(s) 367
MmeI TCCRAC 1 cut(s) 176
MnlI CCTC 2 cut(s) 434, 467
MroI TCCGGA 1 cut(s) 267
MspA1I CMGCKG 2 cut(s) 98, 326
MspI CCGG 1 cut(s) 268
MspR9I CCNGG 1 cut(s) 305
MvaI CCWGG 1 cut(s) 305
MwoI GCNNNNNNNGC 3 cut(s) 237, 408, 503
NdeII GATC 1 cut(s) 148
NheI GCTAGC 1 cut(s) 497
NlaIII CATG 3 cut(s) 16, 127, 411
NlaIV GGNNCC 1 cut(s) 445
NmuCI GTSAC 1 cut(s) 7
NspI RCATGY 1 cut(s) 411
PasI CCCWGGG 1 cut(s) 304
PfeI GAWTC 1 cut(s) 385
PkrI GCNGC 4 cut(s) 40, 97, 328, 608
PsiI TTATAA 1 cut(s) 372
Psp124BI GAGCTC 1 cut(s) 275
Psp6I CCWGG 1 cut(s) 303
PspGI CCWGG 1 cut(s) 303
PspN4I GGNNCC 1 cut(s) 445
PstI CTGCAG 1 cut(s) 97
PvuII CAGCTG 2 cut(s) 98, 326
RsaI GTAC 3 cut(s) 140, 310, 626
RsaNI GTAC 3 cut(s) 139, 309, 625
SacI GAGCTC 1 cut(s) 275
SatI GCNGC 4 cut(s) 39, 96, 327, 607
Sau3AI GATC 1 cut(s) 148
ScaI AGTACT 1 cut(s) 626
ScrFI CCNGG 1 cut(s) 305
SduI GDGCHC 1 cut(s) 275
SfaNI GCATC 1 cut(s) 364
SfcI CTRYAG 2 cut(s) 93, 345
Sse9I AATT 1 cut(s) 367
SsiI CCGC 2 cut(s) 203, 438
SspMI CTAG 2 cut(s) 498, 628
SstI GAGCTC 1 cut(s) 275
StyD4I CCNGG 1 cut(s) 303
TaaI ACNGT 1 cut(s) 474
TaiI ACGT 1 cut(s) 558
TaqI TCGA 1 cut(s) 540
TasI AATT 1 cut(s) 367
TatI WGTACW 1 cut(s) 624
TfiI GAWTC 1 cut(s) 385
TscAI CASTG 1 cut(s) 48
TseFI GTSAC 1 cut(s) 7
TseI GCWGC 4 cut(s) 38, 95, 326, 606
Tsp45I GTSAC 1 cut(s) 7
TspDTI ATGAA 2 cut(s) 29, 478
TspRI CASTG 1 cut(s) 48
XceI RCATGY 1 cut(s) 411
XspI CTAG 2 cut(s) 498, 628
ZrmI AGTACT 1 cut(s) 626
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.