MD15G1112800.v1.1

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0002236
Physical Location & Seq
Reverse (-)
7928017 .. 7931377
3361 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1112800.v1.1.491

Sequence Viewer

Length: 606 bp
ATGGAGGTGACCGTGAAGCAGATGTCTTTGACAGTGGCACTTTTTGGTGTTCTCTCATTCGTTTTCGGAGTTATTGCCGAAAACAAGAAGCCAGCAGTTGGAACTCCTATCGCTGGGAAAGATGTTGTGATCTGCAAGTATCCTACAGATCTATCAGTTGTCTTCGGGTATCTGTCTTTTGCCTTCCTCCTGGTATCTACAGTCGCTGGATTCTTGTCGTTGTTTTACCCATACAAAGGGAAGTCCCTCCCACAAGCTGCTTTGTTCCGAAGCACTAGTTTCGTCATATTCTTCAACATTTCTCTGCTTACAGCAGGACTAGCTGCGGCTCTGTTGCTATGGCCTACAATAACCGAGCAACTTCATCTCACAAGAAACGTTCATCGCAATCTCAAAACAACCTGTCCAACTGCTAAGACGGGTCTCCTTGGTGGCGGTGCTTTTGTATCCCTTGATGCGTCGCTCTTTTGGTTGGTTGCCCTCATGTTAGCCGACAACGCTCGAGAGGACTATTTTGAGGAGACAGAAAAGGATGTTAAGGGTGAATATGGACAGGTTTCTGCAATCGATTTCGATGGACATGCCAATATTAAAGGAAGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

202

Amino Acids

21.71

Weight (kDa)

6.82

Isoelectric Point (pI)

29.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 59 - 154 8e-19 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 98
AciI CCGC 2 cut(s) 326, 435
AclI AACGTT 1 cut(s) 378
AfiI CCNNNNNNNGG 3 cut(s) 98, 113, 236
AgsI TTSAA 1 cut(s) 295
AhlI ACTAGT 1 cut(s) 275
AjnI CCWGG 1 cut(s) 189
AluBI AGCT 2 cut(s) 257, 323
AluI AGCT 2 cut(s) 257, 323
Alw26I GTCTC 2 cut(s) 428, 515
AlwNI CAGNNNCTG 1 cut(s) 206
Ama87I CYCGRG 1 cut(s) 501
AoxI GGCC 1 cut(s) 341
ApeKI GCWGC 2 cut(s) 257, 323
AsuHPI GGTGA 2 cut(s) 19, 554
AvaI CYCGRG 1 cut(s) 501
BbsI GAAGAC 1 cut(s) 154
BbvI GCAGC 2 cut(s) 244, 310
BccI CCATC 1 cut(s) 569
BcgI CGANNNNNNTGC 4 cut(s) 262, 296, 563, 597
BciT130I CCWGG 1 cut(s) 191
BciVI GTATCC 2 cut(s) 150, 457
BcoDI GTCTC 2 cut(s) 428, 515
BcuI ACTAGT 1 cut(s) 275
BfaI CTAG 2 cut(s) 276, 320
BfmI CTRYAG 2 cut(s) 144, 198
BfuI GTATCC 2 cut(s) 150, 457
BglII AGATCT 1 cut(s) 148
BisI GCNGC 3 cut(s) 258, 324, 327
BlsI GCNGC 3 cut(s) 259, 325, 328
Bme1390I CCNGG 1 cut(s) 191
BmeT110I CYCGRG 1 cut(s) 501
BmrFI CCNGG 1 cut(s) 191
BmsI GCATC 1 cut(s) 445
BpiI GAAGAC 1 cut(s) 154
Bsa29I ATCGAT 1 cut(s) 567
BsaI GGTCTC 1 cut(s) 428
BsaJI CCNNGG 1 cut(s) 427
Bsc4I CCNNNNNNNGG 3 cut(s) 98, 113, 236
BseBI CCWGG 1 cut(s) 191
BseCI ATCGAT 1 cut(s) 567
BseDI CCNNGG 1 cut(s) 427
BseGI GGATG 1 cut(s) 538
BseLI CCNNNNNNNGG 3 cut(s) 98, 113, 236
BseRI GAGGAG 1 cut(s) 533
BseXI GCAGC 2 cut(s) 244, 310
BseYI CCCAGC 1 cut(s) 113
BshFI GGCC 1 cut(s) 343
BshVI ATCGAT 1 cut(s) 567
BsiHKCI CYCGRG 1 cut(s) 501
BslFI GGGAC 1 cut(s) 229
BslI CCNNNNNNNGG 3 cut(s) 98, 113, 236
BsmAI GTCTC 2 cut(s) 428, 515
BsmFI GGGAC 1 cut(s) 229
BsnI GGCC 1 cut(s) 343
Bso31I GGTCTC 1 cut(s) 428
BsoBI CYCGRG 1 cut(s) 501
Bsp143I GATC 2 cut(s) 129, 148
BspACI CCGC 2 cut(s) 326, 435
BspANI GGCC 1 cut(s) 343
BspDI ATCGAT 1 cut(s) 567
BspTNI GGTCTC 1 cut(s) 428
BssECI CCNNGG 1 cut(s) 427
BssMI GATC 2 cut(s) 129, 148
BssT1I CCWWGG 1 cut(s) 427
Bst2UI CCWGG 1 cut(s) 191
Bst4CI ACNGT 3 cut(s) 13, 34, 202
BstC8I GCNNGC 1 cut(s) 93
BstDEI CTNAG 1 cut(s) 414
BstEII GGTNACC 1 cut(s) 7
BstF5I GGATG 1 cut(s) 538
BstKTI GATC 2 cut(s) 132, 151
BstMAI GTCTC 2 cut(s) 428, 515
BstMBI GATC 2 cut(s) 129, 148
BstMWI GCNNNNNNNGC 2 cut(s) 320, 497
BstNI CCWGG 1 cut(s) 191
BstNSI RCATGY 1 cut(s) 584
BstPI GGTNACC 1 cut(s) 7
BstSCI CCNGG 1 cut(s) 189
BstSFI CTRYAG 2 cut(s) 144, 198
BstV1I GCAGC 2 cut(s) 244, 310
BstV2I GAAGAC 1 cut(s) 154
BstX2I RGATCY 1 cut(s) 148
BstYI RGATCY 1 cut(s) 148
Bsu15I ATCGAT 1 cut(s) 567
BsuI GTATCC 2 cut(s) 150, 457
BsuRI GGCC 1 cut(s) 343
BsuTUI ATCGAT 1 cut(s) 567
BtgZI GCGATG 1 cut(s) 368
BtsCI GGATG 1 cut(s) 538
BtsIMutI CAGTG 1 cut(s) 39
Cac8I GCNNGC 1 cut(s) 93
CaiI CAGNNNCTG 1 cut(s) 206
ClaI ATCGAT 1 cut(s) 567
CseI GACGC 1 cut(s) 447
CviAII CATG 2 cut(s) 484, 581
CviJI RGCY 6 cut(s) 91, 257, 323, 329, 343, 491
CviKI_1 RGCY 6 cut(s) 91, 257, 323, 329, 343, 491
DdeI CTNAG 1 cut(s) 414
DpnI GATC 2 cut(s) 131, 150
DpnII GATC 2 cut(s) 129, 148
Eco130I CCWWGG 1 cut(s) 427
Eco31I GGTCTC 1 cut(s) 428
Eco88I CYCGRG 1 cut(s) 501
Eco91I GGTNACC 1 cut(s) 7
EcoO65I GGTNACC 1 cut(s) 7
EcoRII CCWGG 1 cut(s) 189
EcoT14I CCWWGG 1 cut(s) 427
ErhI CCWWGG 1 cut(s) 427
FaeI CATG 2 cut(s) 487, 584
FaiI YATR 6 cut(s) 232, 287, 340, 485, 549, 582
FaqI GGGAC 1 cut(s) 229
FatI CATG 2 cut(s) 483, 580
Fnu4HI GCNGC 3 cut(s) 258, 324, 327
FokI GGATG 1 cut(s) 545
Fsp4HI GCNGC 3 cut(s) 258, 324, 327
FspBI CTAG 2 cut(s) 276, 320
GluI GCNGC 3 cut(s) 258, 324, 327
GsaI CCCAGC 1 cut(s) 117
HaeIII GGCC 1 cut(s) 343
HgaI GACGC 1 cut(s) 447
Hin1II CATG 2 cut(s) 487, 584
HinfI GANTC 1 cut(s) 210
HphI GGTGA 2 cut(s) 19, 554
Hpy188I TCNGA 2 cut(s) 68, 269
Hpy188III TCNNGA 1 cut(s) 503
Hpy99I CGWCG 1 cut(s) 463
HpyAV CCTTC 1 cut(s) 193
HpyCH4III ACNGT 3 cut(s) 13, 34, 202
HpyCH4IV ACGT 1 cut(s) 378
HpyCH4V TGCA 2 cut(s) 135, 563
HpyF10VI GCNNNNNNNGC 2 cut(s) 320, 497
HpyF3I CTNAG 1 cut(s) 414
HpySE526I ACGT 1 cut(s) 378
Hsp92II CATG 2 cut(s) 487, 584
Kzo9I GATC 2 cut(s) 129, 148
LpnPI CCDG 8 cut(s) 99, 105, 176, 192, 203, 300, 415, 539
Lsp1109I GCAGC 2 cut(s) 244, 310
LweI GCATC 1 cut(s) 445
MaeI CTAG 2 cut(s) 276, 320
MaeII ACGT 1 cut(s) 378
MaeIII GTNAC 1 cut(s) 7
MalI GATC 2 cut(s) 131, 150
MboI GATC 2 cut(s) 129, 148
MboII GAAGA 2 cut(s) 154, 283
MflI RGATCY 1 cut(s) 148
MmeI TCCRAC 2 cut(s) 79, 431
MnlI CCTC 5 cut(s) 197, 257, 491, 499, 511
MseI TTAA 2 cut(s) 537, 591
MspR9I CCNGG 1 cut(s) 191
MvaI CCWGG 1 cut(s) 191
MwoI GCNNNNNNNGC 2 cut(s) 320, 497
NdeII GATC 2 cut(s) 129, 148
NlaIII CATG 2 cut(s) 487, 584
NmuCI GTSAC 1 cut(s) 7
NspI RCATGY 1 cut(s) 584
PaeR7I CTCGAG 1 cut(s) 501
PfeI GAWTC 1 cut(s) 210
PflMI CCANNNNNTGG 1 cut(s) 98
PkrI GCNGC 3 cut(s) 259, 325, 328
Psp1406I AACGTT 1 cut(s) 378
Psp6I CCWGG 1 cut(s) 189
PspEI GGTNACC 1 cut(s) 7
PspFI CCCAGC 1 cut(s) 113
PspGI CCWGG 1 cut(s) 189
PstNI CAGNNNCTG 1 cut(s) 206
PsuI RGATCY 1 cut(s) 148
SaqAI TTAA 2 cut(s) 537, 591
SatI GCNGC 3 cut(s) 258, 324, 327
Sau3AI GATC 2 cut(s) 129, 148
ScrFI CCNGG 1 cut(s) 191
SetI ASST 6 cut(s) 9, 259, 325, 381, 404, 558
SfaNI GCATC 1 cut(s) 445
SfcI CTRYAG 2 cut(s) 144, 198
Sfr274I CTCGAG 1 cut(s) 501
SlaI CTCGAG 1 cut(s) 501
SmlI CTYRAG 1 cut(s) 501
SmoI CTYRAG 1 cut(s) 501
SpeI ACTAGT 1 cut(s) 275
SsiI CCGC 2 cut(s) 326, 435
SspI AATATT 1 cut(s) 589
SspMI CTAG 2 cut(s) 276, 320
StyD4I CCNGG 1 cut(s) 189
StyI CCWWGG 1 cut(s) 427
TaaI ACNGT 3 cut(s) 13, 34, 202
TaiI ACGT 1 cut(s) 381
TaqI TCGA 3 cut(s) 502, 567, 573
TauI GCSGC 1 cut(s) 329
TfiI GAWTC 1 cut(s) 210
Tru1I TTAA 2 cut(s) 537, 591
Tru9I TTAA 2 cut(s) 537, 591
TscAI CASTG 1 cut(s) 39
TseFI GTSAC 1 cut(s) 7
TseI GCWGC 2 cut(s) 257, 323
Tsp45I GTSAC 1 cut(s) 7
TspDTI ATGAA 2 cut(s) 353, 371
TspRI CASTG 1 cut(s) 39
Van91I CCANNNNNTGG 1 cut(s) 98
XceI RCATGY 1 cut(s) 584
XhoI CTCGAG 1 cut(s) 501
XspI CTAG 2 cut(s) 276, 320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.