RLG00000010330

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0002236
Physical Location & Seq
Forward (+)
730995 .. 734440
3446 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010330

Sequence Viewer

Length: 813 bp
ATGCAAGAGAAGACTACTTTGAAGAATTGGATAGTGTCGAAAAACCTAAACACATTGAGAAAGCTGAAGCTGCACATACCAAGGCACTTGAAGACGATGACAGCAGCGAGATTAGCTCCCGGTAGGACGGAGATTCCCAAATCCTCTCTCTTTTCAGAATCGAATCAAGACGACCCCTTTTTATCCCGATTCAATTTCCCCAGGGCGATGGCTGTGACGGTGAAGCAGATGGCTATGATAGTGGCCTTTTTTGGTGTACTGTCTTTCATCTTCGGGATTATTGCCGAAAACAAGAAGCCGGCAGCTGGAAAACCAGTGCCATGGAAAGATGTTGTTATTTGCAAGTATCCTTCTGATCCTTCGGTGGCGTTGGGATATCTGTCATTTGCATTTCTCCTTATATCTACGGTGGTTGGGTTCTTGTCACTGTTTTACCCATATCAAGGGAAGTCCCTTCCACAAGCTGCCTTGTTCCGGAGCAAGAGTTTTGTTGTCTTCTTCAACATTTCTTTGCTTACAGCAGGACTAGCTGCAGCTCTGTTGCTGTGGACTACAATAACGGAGCAACTTCATCTATCACGAAATGTCCATCACAATCTTAAAACAGACTGCCCCACTGCTAAGACCGGTCTCCTTGGCGGTGGTGCATTTGTTTCCCTTGATTCATCCCTTTTCTGGTTGGTTGCCCTCATGTTGGCCGACAATGCGCGAGAGGACTACTTTGAGGAGTCGGATAAGGATGTCAAGGGTGGAGGGTATACAGAGGTACATGGAGCTGATTATGGACAACATGTCATAAAAGGTAGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

271

Amino Acids

29.78

Weight (kDa)

9.5

Isoelectric Point (pI)

33.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 128 - 223 2.2e-18 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 758
AccII CGCG 1 cut(s) 709
AccIII TCCGGA 1 cut(s) 474
AciI CCGC 1 cut(s) 639
AclWI GGATC 1 cut(s) 350
AcoI YGGCCR 1 cut(s) 696
AcuI CTGAAG 1 cut(s) 86
AfaI GTAC 2 cut(s) 258, 768
AfiI CCNNNNNNNGG 5 cut(s) 305, 443, 474, 675, 694
AflIII ACRYGT 1 cut(s) 790
AgeI ACCGGT 1 cut(s) 626
AgsI TTSAA 4 cut(s) 22, 91, 193, 502
AhdI GACNNNNNGTC 1 cut(s) 791
AjnI CCWGG 1 cut(s) 200
AluBI AGCT 8 cut(s) 64, 70, 116, 305, 464, 530, 536, 776
AluI AGCT 8 cut(s) 64, 70, 116, 305, 464, 530, 536, 776
Alw26I GTCTC 1 cut(s) 635
AlwI GGATC 1 cut(s) 350
Aor13HI TCCGGA 1 cut(s) 474
AoxI GGCC 2 cut(s) 243, 696
ApeKI GCWGC 6 cut(s) 70, 104, 302, 464, 530, 533
AsiGI ACCGGT 1 cut(s) 626
AspLEI GCGC 1 cut(s) 709
AsuC2I CCSGG 1 cut(s) 120
AsuHPI GGTGA 1 cut(s) 232
BbsI GAAGAC 3 cut(s) 17, 98, 487
BbvI GCAGC 6 cut(s) 57, 116, 314, 451, 517, 545
BccI CCATC 3 cut(s) 202, 223, 597
BciT130I CCWGG 1 cut(s) 202
BciVI GTATCC 1 cut(s) 357
BcnI CCSGG 1 cut(s) 120
BcoDI GTCTC 1 cut(s) 635
BfaI CTAG 1 cut(s) 527
BfmI CTRYAG 1 cut(s) 531
BfuI GTATCC 1 cut(s) 357
BisI GCNGC 6 cut(s) 71, 105, 303, 465, 531, 534
BlsI GCNGC 6 cut(s) 72, 106, 304, 466, 532, 535
Bme1390I CCNGG 2 cut(s) 120, 202
BmeRI GACNNNNNGTC 1 cut(s) 791
BmrFI CCNGG 2 cut(s) 120, 202
BpiI GAAGAC 3 cut(s) 17, 98, 487
BplI GAGNNNNNCTC 2 cut(s) 100, 132
BpuMI CCSGG 1 cut(s) 120
BsaI GGTCTC 1 cut(s) 635
BsaJI CCNNGG 5 cut(s) 80, 200, 201, 320, 634
BsaWI WCCGGW 2 cut(s) 474, 626
BsaXI ACNNNNNCTCC 2 cut(s) 469, 499
Bsc4I CCNNNNNNNGG 5 cut(s) 305, 443, 474, 675, 694
Bse118I RCCGGY 2 cut(s) 298, 626
Bse1I ACTGG 1 cut(s) 314
BseAI TCCGGA 1 cut(s) 474
BseBI CCWGG 1 cut(s) 202
BseDI CCNNGG 5 cut(s) 80, 200, 201, 320, 634
BseGI GGATG 2 cut(s) 665, 745
BseLI CCNNNNNNNGG 5 cut(s) 305, 443, 474, 675, 694
BseNI ACTGG 1 cut(s) 314
BseRI GAGGAG 1 cut(s) 740
BseXI GCAGC 6 cut(s) 57, 116, 314, 451, 517, 545
BsgI GTGCAG 1 cut(s) 56
Bsh1236I CGCG 1 cut(s) 709
BshFI GGCC 2 cut(s) 245, 698
BshTI ACCGGT 1 cut(s) 626
BsiSI CCGG 4 cut(s) 120, 299, 475, 627
BslFI GGGAC 1 cut(s) 436
BslI CCNNNNNNNGG 5 cut(s) 305, 443, 474, 675, 694
BsmAI GTCTC 1 cut(s) 635
BsmFI GGGAC 1 cut(s) 436
BsnI GGCC 2 cut(s) 245, 698
Bso31I GGTCTC 1 cut(s) 635
Bsp13I TCCGGA 1 cut(s) 474
Bsp143I GATC 1 cut(s) 355
Bsp19I CCATGG 1 cut(s) 320
BspACI CCGC 1 cut(s) 639
BspANI GGCC 2 cut(s) 245, 698
BspEI TCCGGA 1 cut(s) 474
BspFNI CGCG 1 cut(s) 709
BspMAI CTGCAG 1 cut(s) 535
BspPI GGATC 1 cut(s) 350
BspTNI GGTCTC 1 cut(s) 635
BsrFI RCCGGY 2 cut(s) 298, 626
BsrI ACTGG 1 cut(s) 314
BssAI RCCGGY 2 cut(s) 298, 626
BssECI CCNNGG 5 cut(s) 80, 200, 201, 320, 634
BssMI GATC 1 cut(s) 355
BssNAI GTATAC 1 cut(s) 759
BssT1I CCWWGG 3 cut(s) 80, 320, 634
Bst1107I GTATAC 1 cut(s) 759
Bst2UI CCWGG 1 cut(s) 202
Bst4CI ACNGT 4 cut(s) 220, 261, 409, 429
BstC8I GCNNGC 1 cut(s) 300
BstDEI CTNAG 1 cut(s) 621
BstDSI CCRYGG 1 cut(s) 320
BstF5I GGATG 2 cut(s) 665, 745
BstFNI CGCG 1 cut(s) 709
BstHHI GCGC 1 cut(s) 709
BstKTI GATC 1 cut(s) 358
BstMAI GTCTC 1 cut(s) 635
BstMBI GATC 1 cut(s) 355
BstMWI GCNNNNNNNGC 4 cut(s) 70, 113, 527, 704
BstNI CCWGG 1 cut(s) 202
BstNSI RCATGY 1 cut(s) 794
BstSCI CCNGG 2 cut(s) 118, 200
BstSFI CTRYAG 1 cut(s) 531
BstUI CGCG 1 cut(s) 709
BstV1I GCAGC 6 cut(s) 57, 116, 314, 451, 517, 545
BstV2I GAAGAC 3 cut(s) 17, 98, 487
BstXI CCANNNNNNTGG 2 cut(s) 208, 321
BstZ17I GTATAC 1 cut(s) 759
BsuI GTATCC 1 cut(s) 357
BsuRI GGCC 2 cut(s) 245, 698
BtgI CCRYGG 1 cut(s) 320
BtgZI GCGATG 1 cut(s) 221
BtsCI GGATG 2 cut(s) 665, 745
BtsI GCAGTG 1 cut(s) 615
BtsIMutI CAGTG 3 cut(s) 321, 425, 615
Cac8I GCNNGC 1 cut(s) 300
CfoI GCGC 1 cut(s) 709
Cfr10I RCCGGY 2 cut(s) 298, 626
Csp6I GTAC 2 cut(s) 257, 767
CspAI ACCGGT 1 cut(s) 626
CviAII CATG 4 cut(s) 321, 691, 770, 791
CviQI GTAC 2 cut(s) 257, 767
DdeI CTNAG 1 cut(s) 621
DpnI GATC 1 cut(s) 357
DpnII GATC 1 cut(s) 355
DriI GACNNNNNGTC 1 cut(s) 791
EaeI YGGCCR 1 cut(s) 696
Eam1105I GACNNNNNGTC 1 cut(s) 791
Eco130I CCWWGG 3 cut(s) 80, 320, 634
Eco31I GGTCTC 1 cut(s) 635
Eco32I GATATC 1 cut(s) 377
Eco57I CTGAAG 1 cut(s) 86
EcoRII CCWGG 1 cut(s) 200
EcoRV GATATC 1 cut(s) 377
EcoT14I CCWWGG 3 cut(s) 80, 320, 634
ErhI CCWWGG 3 cut(s) 80, 320, 634
FaeI CATG 4 cut(s) 324, 694, 773, 794
FaqI GGGAC 1 cut(s) 436
FatI CATG 4 cut(s) 320, 690, 769, 790
FblI GTMKAC 1 cut(s) 758
Fnu4HI GCNGC 6 cut(s) 71, 105, 303, 465, 531, 534
FokI GGATG 2 cut(s) 652, 752
Fsp4HI GCNGC 6 cut(s) 71, 105, 303, 465, 531, 534
FspBI CTAG 1 cut(s) 527
GlaI GCGC 1 cut(s) 708
GluI GCNGC 6 cut(s) 71, 105, 303, 465, 531, 534
HaeIII GGCC 2 cut(s) 245, 698
HapII CCGG 4 cut(s) 120, 299, 475, 627
HhaI GCGC 1 cut(s) 709
Hin1II CATG 4 cut(s) 324, 694, 773, 794
Hin6I GCGC 1 cut(s) 707
HinP1I GCGC 1 cut(s) 707
HinfI GANTC 6 cut(s) 133, 158, 163, 189, 662, 728
HpaII CCGG 4 cut(s) 120, 299, 475, 627
HphI GGTGA 1 cut(s) 232
Hpy166II GTNNAC 3 cut(s) 257, 549, 759
Hpy188I TCNGA 3 cut(s) 157, 355, 733
Hpy188III TCNNGA 5 cut(s) 167, 186, 274, 475, 579
Hpy8I GTNNAC 3 cut(s) 257, 549, 759
HpyAV CCTTC 3 cut(s) 360, 369, 464
HpyCH4III ACNGT 4 cut(s) 220, 261, 409, 429
HpyCH4V TGCA 6 cut(s) 4, 73, 342, 389, 533, 647
HpyF10VI GCNNNNNNNGC 4 cut(s) 70, 113, 527, 704
HpyF3I CTNAG 1 cut(s) 621
Hsp92II CATG 4 cut(s) 324, 694, 773, 794
HspAI GCGC 1 cut(s) 707
Kpn2I TCCGGA 1 cut(s) 474
KroI GCCGGC 1 cut(s) 298
KroNI GCCGGC 1 cut(s) 300
Kzo9I GATC 1 cut(s) 355
LmnI GCTCC 4 cut(s) 121, 477, 562, 773
Lsp1109I GCAGC 6 cut(s) 57, 116, 314, 451, 517, 545
MaeI CTAG 1 cut(s) 527
MaeIII GTNAC 2 cut(s) 214, 423
MalI GATC 1 cut(s) 357
MboI GATC 1 cut(s) 355
MboII GAAGA 6 cut(s) 22, 34, 103, 262, 487, 490
MluCI AATT 2 cut(s) 25, 193
MlyI GAGTC 1 cut(s) 737
MmeI TCCRAC 1 cut(s) 711
MnlI CCTC 6 cut(s) 154, 698, 706, 718, 746, 757
MroI TCCGGA 1 cut(s) 474
MroNI GCCGGC 1 cut(s) 298
MseI TTAA 1 cut(s) 600
MspA1I CMGCKG 1 cut(s) 305
MspI CCGG 4 cut(s) 120, 299, 475, 627
MspR9I CCNGG 2 cut(s) 120, 202
MvaI CCWGG 1 cut(s) 202
MvnI CGCG 1 cut(s) 709
MwoI GCNNNNNNNGC 4 cut(s) 70, 113, 527, 704
NaeI GCCGGC 1 cut(s) 300
NciI CCSGG 1 cut(s) 120
NcoI CCATGG 1 cut(s) 320
NdeII GATC 1 cut(s) 355
NgoMIV GCCGGC 1 cut(s) 298
NlaIII CATG 4 cut(s) 324, 694, 773, 794
NmuCI GTSAC 2 cut(s) 214, 423
NspI RCATGY 1 cut(s) 794
PasI CCCWGGG 1 cut(s) 201
PciI ACATGT 1 cut(s) 790
PdiI GCCGGC 1 cut(s) 300
PfeI GAWTC 5 cut(s) 133, 158, 163, 189, 662
PinAI ACCGGT 1 cut(s) 626
PkrI GCNGC 6 cut(s) 72, 106, 304, 466, 532, 535
PleI GAGTC 1 cut(s) 736
PpsI GAGTC 1 cut(s) 736
PscI ACATGT 1 cut(s) 790
Psp6I CCWGG 1 cut(s) 200
PspGI CCWGG 1 cut(s) 200
PstI CTGCAG 1 cut(s) 535
PvuII CAGCTG 1 cut(s) 305
RsaI GTAC 2 cut(s) 258, 768
RsaNI GTAC 2 cut(s) 257, 767
SaqAI TTAA 1 cut(s) 600
SatI GCNGC 6 cut(s) 71, 105, 303, 465, 531, 534
Sau3AI GATC 1 cut(s) 355
SchI GAGTC 1 cut(s) 737
ScrFI CCNGG 2 cut(s) 120, 202
SfcI CTRYAG 1 cut(s) 531
Sse9I AATT 2 cut(s) 25, 193
SsiI CCGC 1 cut(s) 639
SspMI CTAG 1 cut(s) 527
StyD4I CCNGG 2 cut(s) 118, 200
StyI CCWWGG 3 cut(s) 80, 320, 634
TaaI ACNGT 4 cut(s) 220, 261, 409, 429
TaqI TCGA 2 cut(s) 38, 161
TasI AATT 2 cut(s) 25, 193
TatI WGTACW 1 cut(s) 256
TfiI GAWTC 5 cut(s) 133, 158, 163, 189, 662
Tru1I TTAA 1 cut(s) 600
Tru9I TTAA 1 cut(s) 600
TscAI CASTG 3 cut(s) 321, 432, 622
TseFI GTSAC 2 cut(s) 214, 423
TseI GCWGC 6 cut(s) 70, 104, 302, 464, 530, 533
Tsp45I GTSAC 2 cut(s) 214, 423
TspDTI ATGAA 3 cut(s) 256, 560, 654
TspGWI ACGGA 2 cut(s) 143, 575
TspRI CASTG 3 cut(s) 321, 432, 622
XceI RCATGY 1 cut(s) 794
XmiI GTMKAC 1 cut(s) 758
XspI CTAG 1 cut(s) 527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.