Rroxscaffold_7G00156830

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0002236
Physical Location & Seq
Forward (+)
747164 .. 749286
2123 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00156830.1

Sequence Viewer

Length: 606 bp
ATGGCTGTGACGGTGAAGCAGATGGCTATGATAGTGGCCTTTTTTGGTGTACTGTCTTTCATCTTCGGGATTATTGCGGAAAACAAGAAGCCGGCAGCTGGAAACCCAGTGCCATGGAAAGATGTTGTTATCTGCAAGTATCCTTCTGATCCTTCGGTGGCGTTGGGATATCTGTCATTTGCATTTCTCCTTGTATCTACAGTGGTTGGGTTCTTGTCACTGTTTTACCCATATCAAGGGAAGTCCCTTCCGCAAGCTGCTTTGTTCCGGAGCAAGAGTTTCGTTGTCTTCTTCAACATTTCCTTGCTTACAGCAGGACTAGCCGCAGCTCTGTTGCTGTGGACTACAATAACGGAGCAACTTCATCTATCACGAAATGTCCATCACAATCTTAAAACAGACTGCCCCACTGCTAAGACTGGTCTCCTTGGTGGTGGTGCATTTGTATCCCTTGATTCATCCCTTTTCTGGTTGGTTGCCCTCATGCTAGCCGACAATGCTCGAGAGGACTACTTCGAGGAGTCGGATAAGGATGTCAAGGGTAGTGGGTATACAGAGGTACACGGAGCTGATTATGGACAACATGTTGTAAAAGGTAGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

201

Amino Acids

21.76

Weight (kDa)

6.96

Isoelectric Point (pI)

32.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 59 - 154 1.1e-18 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 551
AccIII TCCGGA 1 cut(s) 267
AciI CCGC 3 cut(s) 77, 251, 324
AclWI GGATC 1 cut(s) 143
AfaI GTAC 2 cut(s) 51, 561
AfiI CCNNNNNNNGG 3 cut(s) 98, 236, 468
AflIII ACRYGT 1 cut(s) 583
AgsI TTSAA 1 cut(s) 295
AluBI AGCT 4 cut(s) 98, 257, 329, 569
AluI AGCT 4 cut(s) 98, 257, 329, 569
Alw26I GTCTC 1 cut(s) 428
AlwI GGATC 1 cut(s) 143
Ama87I CYCGRG 1 cut(s) 501
Aor13HI TCCGGA 1 cut(s) 267
AoxI GGCC 1 cut(s) 36
ApeKI GCWGC 3 cut(s) 95, 257, 326
AsuHPI GGTGA 1 cut(s) 25
AsuNHI GCTAGC 1 cut(s) 487
AvaI CYCGRG 1 cut(s) 501
BbsI GAAGAC 1 cut(s) 280
BbvI GCAGC 3 cut(s) 107, 244, 338
BccI CCATC 2 cut(s) 16, 390
BcgI CGANNNNNNTGC 2 cut(s) 262, 296
BciVI GTATCC 2 cut(s) 150, 457
BcoDI GTCTC 1 cut(s) 428
BfaI CTAG 2 cut(s) 320, 488
BfmI CTRYAG 1 cut(s) 198
BfuI GTATCC 2 cut(s) 150, 457
BisI GCNGC 4 cut(s) 96, 258, 324, 327
BlsI GCNGC 4 cut(s) 97, 259, 325, 328
BmeT110I CYCGRG 1 cut(s) 501
BmrI ACTGGG 1 cut(s) 101
BmtI GCTAGC 1 cut(s) 491
BmuI ACTGGG 1 cut(s) 101
BpiI GAAGAC 1 cut(s) 280
BsaI GGTCTC 1 cut(s) 428
BsaJI CCNNGG 2 cut(s) 113, 427
BsaWI WCCGGW 1 cut(s) 267
BsaXI ACNNNNNCTCC 2 cut(s) 262, 292
Bsc4I CCNNNNNNNGG 3 cut(s) 98, 236, 468
Bse118I RCCGGY 1 cut(s) 91
Bse1I ACTGG 2 cut(s) 107, 424
BseAI TCCGGA 1 cut(s) 267
BseDI CCNNGG 2 cut(s) 113, 427
BseGI GGATG 2 cut(s) 458, 538
BseLI CCNNNNNNNGG 3 cut(s) 98, 236, 468
BseNI ACTGG 2 cut(s) 107, 424
BseRI GAGGAG 1 cut(s) 533
BseXI GCAGC 3 cut(s) 107, 244, 338
BshFI GGCC 1 cut(s) 38
BsiHKCI CYCGRG 1 cut(s) 501
BsiSI CCGG 2 cut(s) 92, 268
BslFI GGGAC 1 cut(s) 229
BslI CCNNNNNNNGG 3 cut(s) 98, 236, 468
BsmAI GTCTC 1 cut(s) 428
BsmFI GGGAC 1 cut(s) 229
BsnI GGCC 1 cut(s) 38
Bso31I GGTCTC 1 cut(s) 428
BsoBI CYCGRG 1 cut(s) 501
Bsp13I TCCGGA 1 cut(s) 267
Bsp143I GATC 1 cut(s) 148
Bsp19I CCATGG 1 cut(s) 113
BspACI CCGC 3 cut(s) 77, 251, 324
BspANI GGCC 1 cut(s) 38
BspEI TCCGGA 1 cut(s) 267
BspOI GCTAGC 1 cut(s) 491
BspPI GGATC 1 cut(s) 143
BspTNI GGTCTC 1 cut(s) 428
BsrFI RCCGGY 1 cut(s) 91
BsrI ACTGG 2 cut(s) 107, 424
BssAI RCCGGY 1 cut(s) 91
BssECI CCNNGG 2 cut(s) 113, 427
BssMI GATC 1 cut(s) 148
BssNAI GTATAC 1 cut(s) 552
BssT1I CCWWGG 2 cut(s) 113, 427
Bst1107I GTATAC 1 cut(s) 552
Bst4CI ACNGT 4 cut(s) 13, 54, 202, 222
BstC8I GCNNGC 3 cut(s) 93, 255, 489
BstDEI CTNAG 1 cut(s) 414
BstDSI CCRYGG 1 cut(s) 113
BstF5I GGATG 2 cut(s) 458, 538
BstKTI GATC 1 cut(s) 151
BstMAI GTCTC 1 cut(s) 428
BstMBI GATC 1 cut(s) 148
BstMWI GCNNNNNNNGC 2 cut(s) 320, 497
BstNSI RCATGY 1 cut(s) 587
BstSFI CTRYAG 1 cut(s) 198
BstV1I GCAGC 3 cut(s) 107, 244, 338
BstV2I GAAGAC 1 cut(s) 280
BstXI CCANNNNNNTGG 1 cut(s) 114
BstZ17I GTATAC 1 cut(s) 552
BsuI GTATCC 2 cut(s) 150, 457
BsuRI GGCC 1 cut(s) 38
BtgI CCRYGG 1 cut(s) 113
BtsCI GGATG 2 cut(s) 458, 538
BtsI GCAGTG 1 cut(s) 408
BtsIMutI CAGTG 4 cut(s) 114, 207, 218, 408
Cac8I GCNNGC 3 cut(s) 93, 255, 489
Cfr10I RCCGGY 1 cut(s) 91
Csp6I GTAC 2 cut(s) 50, 560
CspCI CAANNNNNGTGG 2 cut(s) 526, 561
CviAII CATG 3 cut(s) 114, 484, 584
CviQI GTAC 2 cut(s) 50, 560
DdeI CTNAG 1 cut(s) 414
DpnI GATC 1 cut(s) 150
DpnII GATC 1 cut(s) 148
Eco130I CCWWGG 2 cut(s) 113, 427
Eco31I GGTCTC 1 cut(s) 428
Eco32I GATATC 1 cut(s) 170
Eco88I CYCGRG 1 cut(s) 501
EcoRV GATATC 1 cut(s) 170
EcoT14I CCWWGG 2 cut(s) 113, 427
ErhI CCWWGG 2 cut(s) 113, 427
FaeI CATG 3 cut(s) 117, 487, 587
FaiI YATR 7 cut(s) 29, 115, 232, 485, 552, 576, 585
FaqI GGGAC 1 cut(s) 229
FatI CATG 3 cut(s) 113, 483, 583
FblI GTMKAC 1 cut(s) 551
Fnu4HI GCNGC 4 cut(s) 96, 258, 324, 327
FokI GGATG 2 cut(s) 445, 545
Fsp4HI GCNGC 4 cut(s) 96, 258, 324, 327
FspBI CTAG 2 cut(s) 320, 488
GluI GCNGC 4 cut(s) 96, 258, 324, 327
HaeIII GGCC 1 cut(s) 38
HapII CCGG 2 cut(s) 92, 268
Hin1II CATG 3 cut(s) 117, 487, 587
HinfI GANTC 2 cut(s) 455, 521
HpaII CCGG 2 cut(s) 92, 268
HphI GGTGA 1 cut(s) 25
Hpy166II GTNNAC 4 cut(s) 50, 342, 552, 562
Hpy188I TCNGA 2 cut(s) 148, 526
Hpy188III TCNNGA 4 cut(s) 67, 268, 372, 503
Hpy8I GTNNAC 4 cut(s) 50, 342, 552, 562
HpyAV CCTTC 3 cut(s) 153, 162, 257
HpyCH4III ACNGT 4 cut(s) 13, 54, 202, 222
HpyCH4V TGCA 3 cut(s) 135, 182, 440
HpyF10VI GCNNNNNNNGC 2 cut(s) 320, 497
HpyF3I CTNAG 1 cut(s) 414
Hsp92II CATG 3 cut(s) 117, 487, 587
Kpn2I TCCGGA 1 cut(s) 267
KroI GCCGGC 1 cut(s) 91
KroNI GCCGGC 1 cut(s) 93
Kzo9I GATC 1 cut(s) 148
LmnI GCTCC 3 cut(s) 270, 355, 566
LpnPI CCDG 7 cut(s) 84, 105, 120, 281, 300, 405, 454
Lsp1109I GCAGC 3 cut(s) 107, 244, 338
MaeI CTAG 2 cut(s) 320, 488
MaeIII GTNAC 2 cut(s) 7, 216
MalI GATC 1 cut(s) 150
MboI GATC 1 cut(s) 148
MboII GAAGA 3 cut(s) 55, 280, 283
MlyI GAGTC 1 cut(s) 530
MmeI TCCRAC 1 cut(s) 504
MnlI CCTC 4 cut(s) 491, 499, 511, 550
MroI TCCGGA 1 cut(s) 267
MroNI GCCGGC 1 cut(s) 91
MseI TTAA 1 cut(s) 393
MspA1I CMGCKG 1 cut(s) 98
MspI CCGG 2 cut(s) 92, 268
MwoI GCNNNNNNNGC 2 cut(s) 320, 497
NaeI GCCGGC 1 cut(s) 93
NcoI CCATGG 1 cut(s) 113
NdeII GATC 1 cut(s) 148
NgoMIV GCCGGC 1 cut(s) 91
NheI GCTAGC 1 cut(s) 487
NlaIII CATG 3 cut(s) 117, 487, 587
NmuCI GTSAC 2 cut(s) 7, 216
NspI RCATGY 1 cut(s) 587
PaeR7I CTCGAG 1 cut(s) 501
PciI ACATGT 1 cut(s) 583
PdiI GCCGGC 1 cut(s) 93
PfeI GAWTC 1 cut(s) 455
PkrI GCNGC 4 cut(s) 97, 259, 325, 328
PleI GAGTC 1 cut(s) 529
PpsI GAGTC 1 cut(s) 529
PscI ACATGT 1 cut(s) 583
PvuII CAGCTG 1 cut(s) 98
RsaI GTAC 2 cut(s) 51, 561
RsaNI GTAC 2 cut(s) 50, 560
SaqAI TTAA 1 cut(s) 393
SatI GCNGC 4 cut(s) 96, 258, 324, 327
Sau3AI GATC 1 cut(s) 148
SchI GAGTC 1 cut(s) 530
SetI ASST 6 cut(s) 100, 259, 331, 561, 571, 598
SfcI CTRYAG 1 cut(s) 198
Sfr274I CTCGAG 1 cut(s) 501
SlaI CTCGAG 1 cut(s) 501
SmlI CTYRAG 1 cut(s) 501
SmoI CTYRAG 1 cut(s) 501
SsiI CCGC 3 cut(s) 77, 251, 324
SspMI CTAG 2 cut(s) 320, 488
StyI CCWWGG 2 cut(s) 113, 427
TaaI ACNGT 4 cut(s) 13, 54, 202, 222
TaqI TCGA 2 cut(s) 502, 516
TatI WGTACW 1 cut(s) 49
TauI GCSGC 1 cut(s) 326
TfiI GAWTC 1 cut(s) 455
Tru1I TTAA 1 cut(s) 393
Tru9I TTAA 1 cut(s) 393
TscAI CASTG 4 cut(s) 114, 207, 225, 415
TseFI GTSAC 2 cut(s) 7, 216
TseI GCWGC 3 cut(s) 95, 257, 326
Tsp45I GTSAC 2 cut(s) 7, 216
TspDTI ATGAA 3 cut(s) 49, 353, 447
TspGWI ACGGA 2 cut(s) 368, 579
TspRI CASTG 4 cut(s) 114, 207, 225, 415
XceI RCATGY 1 cut(s) 587
XhoI CTCGAG 1 cut(s) 501
XmiI GTMKAC 1 cut(s) 551
XspI CTAG 2 cut(s) 320, 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.