RLG00000035617

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0002236
Physical Location & Seq
Reverse (-)
69369717 .. 69370979
1263 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035617

Sequence Viewer

Length: 627 bp
ATGGCAGTCACCATGAAGATTATGTGTGTGCTTGTTGCAGCACTGGGTATTCTTTCGTTTGTGTTTGGTATTCTCGCTGAAACCAAAAAGCCTGCAGCTGGAGTTCCCATAATAGGGAAAGGCATGGTGATTTGCAAGTACCCATCTGATCCAACGCTTGCTTATGCGTGGCTATCTTTTGGATTTCTTCTGTTATGTTCTGCGGTTGGTTGGTTCTCTGTATTTTATAGCTACAAAGGCAGAAAAGCTCCCACTTCTGTTTTCTTCCGGAGCTCCACCTTCTTCTCCTTCTTCACCATTACCCTGGGTACAATAGGATTAGCAGCTGCATTTTTGCTATGGCCTATAACGACGCATCACCACCCAAATTATAACATTACTGGGAATCAAGACCCCCACAGCGCCACATGCCCAACAGCCAAAACTGGTGTTCTTGGAGGAGGAGCCTTCTTGTCCCTCAACTCATCGCTACTCTGGTTAGTTTGCTTCATCTTGGCTAGCAATGAAAGAGAAGACTACTTTGAAGAGTTGGATAGTGTCGAAAAACCTAAACACATTGAGAAAGCTGAAGCTGGACATACCACGGCACTTGAAGCTGATTACAGCAGCGCGATTATCTCCCGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

209

Amino Acids

22.58

Weight (kDa)

7.67

Isoelectric Point (pI)

35.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 60 - 156 3e-10 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 372
AccII CGCG 1 cut(s) 611
AccIII TCCGGA 1 cut(s) 267
AciI CCGC 1 cut(s) 203
AclWI GGATC 1 cut(s) 143
AcuI CTGAAG 1 cut(s) 588
AfaI GTAC 2 cut(s) 140, 310
AfiI CCNNNNNNNGG 3 cut(s) 98, 113, 114
AgsI TTSAA 2 cut(s) 524, 593
AjnI CCWGG 1 cut(s) 303
AluBI AGCT 8 cut(s) 98, 231, 248, 273, 326, 566, 572, 596
AluI AGCT 8 cut(s) 98, 231, 248, 273, 326, 566, 572, 596
Alw21I GWGCWC 1 cut(s) 275
AlwI GGATC 1 cut(s) 143
Aor13HI TCCGGA 1 cut(s) 267
AoxI GGCC 1 cut(s) 341
ApeKI GCWGC 5 cut(s) 38, 95, 323, 326, 606
AspLEI GCGC 2 cut(s) 404, 611
AsuC2I CCSGG 1 cut(s) 622
AsuHPI GGTGA 3 cut(s) 139, 286, 350
AsuNHI GCTAGC 1 cut(s) 497
BanII GRGCYC 1 cut(s) 275
BbsI GAAGAC 1 cut(s) 519
Bbv12I GWGCWC 1 cut(s) 275
BbvI GCAGC 5 cut(s) 50, 107, 313, 335, 618
BccI CCATC 1 cut(s) 151
BceAI ACGGC 1 cut(s) 600
BciT130I CCWGG 1 cut(s) 305
BcnI CCSGG 1 cut(s) 622
BfaI CTAG 1 cut(s) 498
BfmI CTRYAG 1 cut(s) 93
BfoI RGCGCY 1 cut(s) 405
BisI GCNGC 5 cut(s) 39, 96, 324, 327, 607
BlsI GCNGC 5 cut(s) 40, 97, 325, 328, 608
Bme1390I CCNGG 2 cut(s) 305, 622
BmiI GGNNCC 1 cut(s) 445
BmrFI CCNGG 2 cut(s) 305, 622
BmrI ACTGGG 2 cut(s) 53, 390
BmsI GCATC 1 cut(s) 364
BmtI GCTAGC 1 cut(s) 501
BmuI ACTGGG 2 cut(s) 53, 390
BpiI GAAGAC 1 cut(s) 519
BpmI CTGGAG 1 cut(s) 120
BpuMI CCSGG 1 cut(s) 622
BsaJI CCNNGG 3 cut(s) 303, 304, 582
BsaWI WCCGGW 1 cut(s) 267
Bsc4I CCNNNNNNNGG 3 cut(s) 98, 113, 114
Bse1I ACTGG 3 cut(s) 48, 385, 430
Bse3DI GCAATG 1 cut(s) 508
BseAI TCCGGA 1 cut(s) 267
BseBI CCWGG 1 cut(s) 305
BseDI CCNNGG 3 cut(s) 303, 304, 582
BseLI CCNNNNNNNGG 3 cut(s) 98, 113, 114
BseMI GCAATG 1 cut(s) 508
BseNI ACTGG 3 cut(s) 48, 385, 430
BseRI GAGGAG 2 cut(s) 453, 456
BseXI GCAGC 5 cut(s) 50, 107, 313, 335, 618
Bsh1236I CGCG 1 cut(s) 611
BshFI GGCC 1 cut(s) 343
BsiHKAI GWGCWC 1 cut(s) 275
BsiSI CCGG 2 cut(s) 268, 622
BslFI GGGAC 1 cut(s) 439
BslI CCNNNNNNNGG 3 cut(s) 98, 113, 114
BsmFI GGGAC 1 cut(s) 439
BsnI GGCC 1 cut(s) 343
Bsp1286I GDGCHC 1 cut(s) 275
Bsp13I TCCGGA 1 cut(s) 267
Bsp143I GATC 1 cut(s) 148
BspACI CCGC 1 cut(s) 203
BspANI GGCC 1 cut(s) 343
BspEI TCCGGA 1 cut(s) 267
BspFNI CGCG 1 cut(s) 611
BspLI GGNNCC 1 cut(s) 445
BspMAI CTGCAG 1 cut(s) 97
BspOI GCTAGC 1 cut(s) 501
BspPI GGATC 1 cut(s) 143
BsrDI GCAATG 1 cut(s) 508
BsrI ACTGG 3 cut(s) 48, 385, 430
BssECI CCNNGG 3 cut(s) 303, 304, 582
BssMI GATC 1 cut(s) 148
Bst2UI CCWGG 1 cut(s) 305
Bst6I CTCTTC 1 cut(s) 519
BstC8I GCNNGC 3 cut(s) 93, 159, 499
BstDSI CCRYGG 1 cut(s) 582
BstFNI CGCG 1 cut(s) 611
BstH2I RGCGCY 1 cut(s) 405
BstHHI GCGC 2 cut(s) 404, 611
BstKTI GATC 1 cut(s) 151
BstMBI GATC 1 cut(s) 148
BstMWI GCNNNNNNNGC 3 cut(s) 237, 408, 593
BstNI CCWGG 1 cut(s) 305
BstNSI RCATGY 1 cut(s) 411
BstSCI CCNGG 2 cut(s) 303, 620
BstSFI CTRYAG 1 cut(s) 93
BstUI CGCG 1 cut(s) 611
BstV1I GCAGC 5 cut(s) 50, 107, 313, 335, 618
BstV2I GAAGAC 1 cut(s) 519
BstXI CCANNNNNNTGG 1 cut(s) 304
BsuRI GGCC 1 cut(s) 343
BtgI CCRYGG 1 cut(s) 582
BtgZI GCGATG 1 cut(s) 450
BtsIMutI CAGTG 1 cut(s) 41
Cac8I GCNNGC 3 cut(s) 93, 159, 499
CfoI GCGC 2 cut(s) 404, 611
CseI GACGC 1 cut(s) 361
Csp6I GTAC 2 cut(s) 139, 309
CspCI CAANNNNNGTGG 2 cut(s) 571, 606
CviAII CATG 3 cut(s) 13, 124, 408
CviQI GTAC 2 cut(s) 139, 309
DpnI GATC 1 cut(s) 150
DpnII GATC 1 cut(s) 148
Eam1104I CTCTTC 1 cut(s) 519
EarI CTCTTC 1 cut(s) 519
Ecl136II GAGCTC 1 cut(s) 273
Eco24I GRGCYC 1 cut(s) 275
Eco53kI GAGCTC 1 cut(s) 273
Eco57I CTGAAG 1 cut(s) 588
EcoICRI GAGCTC 1 cut(s) 273
EcoRII CCWGG 1 cut(s) 303
EcoT38I GRGCYC 1 cut(s) 275
FaeI CATG 3 cut(s) 16, 127, 411
FaqI GGGAC 1 cut(s) 439
FatI CATG 3 cut(s) 12, 123, 407
Fnu4HI GCNGC 5 cut(s) 39, 96, 324, 327, 607
FriOI GRGCYC 1 cut(s) 275
Fsp4HI GCNGC 5 cut(s) 39, 96, 324, 327, 607
FspBI CTAG 1 cut(s) 498
GlaI GCGC 2 cut(s) 403, 610
GluI GCNGC 5 cut(s) 39, 96, 324, 327, 607
GsuI CTGGAG 1 cut(s) 120
HaeII RGCGCY 1 cut(s) 405
HaeIII GGCC 1 cut(s) 343
HapII CCGG 2 cut(s) 268, 622
HgaI GACGC 1 cut(s) 361
HhaI GCGC 2 cut(s) 404, 611
Hin1II CATG 3 cut(s) 16, 127, 411
Hin6I GCGC 2 cut(s) 402, 609
HinP1I GCGC 2 cut(s) 402, 609
HinfI GANTC 1 cut(s) 385
HpaII CCGG 2 cut(s) 268, 622
HphI GGTGA 3 cut(s) 139, 286, 350
Hpy188I TCNGA 1 cut(s) 148
Hpy188III TCNNGA 2 cut(s) 268, 389
Hpy99I CGWCG 1 cut(s) 355
HpyAV CCTTC 3 cut(s) 289, 298, 457
HpyCH4V TGCA 4 cut(s) 38, 95, 135, 329
HpyF10VI GCNNNNNNNGC 3 cut(s) 237, 408, 593
Hsp92II CATG 3 cut(s) 16, 127, 411
HspAI GCGC 2 cut(s) 402, 609
Kpn2I TCCGGA 1 cut(s) 267
Kzo9I GATC 1 cut(s) 148
LmnI GCTCC 4 cut(s) 253, 270, 278, 443
Lsp1109I GCAGC 5 cut(s) 50, 107, 313, 335, 618
LweI GCATC 1 cut(s) 364
MaeI CTAG 1 cut(s) 498
MaeIII GTNAC 1 cut(s) 7
MalI GATC 1 cut(s) 150
MboI GATC 1 cut(s) 148
MboII GAAGA 7 cut(s) 28, 179, 256, 274, 283, 524, 536
MhlI GDGCHC 1 cut(s) 275
MluCI AATT 1 cut(s) 367
MmeI TCCRAC 2 cut(s) 176, 510
MnlI CCTC 3 cut(s) 431, 434, 467
MroI TCCGGA 1 cut(s) 267
MspA1I CMGCKG 2 cut(s) 98, 326
MspI CCGG 2 cut(s) 268, 622
MspR9I CCNGG 2 cut(s) 305, 622
MvaI CCWGG 1 cut(s) 305
MvnI CGCG 1 cut(s) 611
MwoI GCNNNNNNNGC 3 cut(s) 237, 408, 593
NciI CCSGG 1 cut(s) 622
NdeII GATC 1 cut(s) 148
NheI GCTAGC 1 cut(s) 497
NlaIII CATG 3 cut(s) 16, 127, 411
NlaIV GGNNCC 1 cut(s) 445
NmuCI GTSAC 1 cut(s) 7
NspI RCATGY 1 cut(s) 411
PasI CCCWGGG 1 cut(s) 304
PfeI GAWTC 1 cut(s) 385
PkrI GCNGC 5 cut(s) 40, 97, 325, 328, 608
PsiI TTATAA 1 cut(s) 372
Psp124BI GAGCTC 1 cut(s) 275
Psp6I CCWGG 1 cut(s) 303
PspGI CCWGG 1 cut(s) 303
PspN4I GGNNCC 1 cut(s) 445
PstI CTGCAG 1 cut(s) 97
PvuII CAGCTG 2 cut(s) 98, 326
RsaI GTAC 2 cut(s) 140, 310
RsaNI GTAC 2 cut(s) 139, 309
SacI GAGCTC 1 cut(s) 275
SatI GCNGC 5 cut(s) 39, 96, 324, 327, 607
Sau3AI GATC 1 cut(s) 148
ScrFI CCNGG 2 cut(s) 305, 622
SduI GDGCHC 1 cut(s) 275
SfaNI GCATC 1 cut(s) 364
SfcI CTRYAG 1 cut(s) 93
Sse9I AATT 1 cut(s) 367
SsiI CCGC 1 cut(s) 203
SspMI CTAG 1 cut(s) 498
SstI GAGCTC 1 cut(s) 275
StyD4I CCNGG 2 cut(s) 303, 620
TaqI TCGA 1 cut(s) 540
TasI AATT 1 cut(s) 367
TfiI GAWTC 1 cut(s) 385
TscAI CASTG 1 cut(s) 48
TseFI GTSAC 1 cut(s) 7
TseI GCWGC 5 cut(s) 38, 95, 323, 326, 606
Tsp45I GTSAC 1 cut(s) 7
TspDTI ATGAA 3 cut(s) 29, 478, 519
TspRI CASTG 1 cut(s) 48
XceI RCATGY 1 cut(s) 411
XspI CTAG 1 cut(s) 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.