MD08G1132600.v1.1

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0002236
Physical Location & Seq
Forward (+)
12536228 .. 12538463
2236 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1132600.v1.1.491

Sequence Viewer

Length: 606 bp
ATGGGTGTGACCGTGAAGCAGATGTCTCTGACAGTGGCACTTTTCGGTGTTCTCTCGTTCATATTCGGAGTTATTGCTGAAAACAAGAAGCCAGCAACTGGAACTCCTATGGTTGGGAAAGATGTTGTGATCTGCAAGTATCCTTCAGATCCATCAGTTGTCCTTGGGTATCTGTCTTTTGCTTTCCTCCTGGTATCTACAGTTGCTGGGTTCTTGTCATTGTTTTACCCATACAAAGGGAAGTCCATTCCACAAGCTGCGTTGTTCCGAAGCACTAGTTTCGTCATATTCCTCAACATTTCTCTGCTTACAGCAGGACTAGCTGCAGCTCTGTTGCTATGGCCTACAGTAACCGAGCAACTTCATCACTCAAGAAAGGTTCATCGCAATCTCCAAACAAAATGCCCAACTGCTAAGACCGGTCTTCTTGGTGGTGGTGCTTTTGTGTCCCTTGACGCATCGCTCTTTTGGTTGGTTGCCCTGATGTTAGCTGACAATGCCCGAGAGGACTATTTTGAGGAGACTGAAAAGGATGTCAAGGGTGAATATGGACAGGTTTCTGCAATCGATTTCGATGGACATGCCAATATTAAAGGAAGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

202

Amino Acids

21.64

Weight (kDa)

8.49

Isoelectric Point (pI)

31.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 59 - 154 2.2e-18 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 98
AclWI GGATC 1 cut(s) 143
AcuI CTGAAG 1 cut(s) 129
AfiI CCNNNNNNNGG 3 cut(s) 98, 113, 236
AgeI ACCGGT 1 cut(s) 419
AhlI ACTAGT 1 cut(s) 275
AjnI CCWGG 1 cut(s) 189
AluBI AGCT 4 cut(s) 257, 323, 329, 491
AluI AGCT 4 cut(s) 257, 323, 329, 491
Alw26I GTCTC 2 cut(s) 30, 515
AlwI GGATC 1 cut(s) 143
AlwNI CAGNNNCTG 2 cut(s) 98, 206
Ama87I CYCGRG 1 cut(s) 501
AoxI GGCC 1 cut(s) 341
ApeKI GCWGC 3 cut(s) 257, 323, 326
AsiGI ACCGGT 1 cut(s) 419
AsuHPI GGTGA 1 cut(s) 554
AvaI CYCGRG 1 cut(s) 501
BbsI GAAGAC 1 cut(s) 416
BbvI GCAGC 3 cut(s) 244, 310, 338
BccI CCATC 2 cut(s) 160, 569
BcgI CGANNNNNNTGC 4 cut(s) 262, 296, 563, 597
BciT130I CCWGG 1 cut(s) 191
BciVI GTATCC 1 cut(s) 150
BcoDI GTCTC 2 cut(s) 30, 515
BcuI ACTAGT 1 cut(s) 275
BfaI CTAG 2 cut(s) 276, 320
BfmI CTRYAG 3 cut(s) 198, 324, 345
BfuI GTATCC 1 cut(s) 150
BisI GCNGC 3 cut(s) 258, 324, 327
BlsI GCNGC 3 cut(s) 259, 325, 328
Bme1390I CCNGG 1 cut(s) 191
BmeT110I CYCGRG 1 cut(s) 501
BmrFI CCNGG 1 cut(s) 191
BmsI GCATC 1 cut(s) 467
BpiI GAAGAC 1 cut(s) 416
BpuEI CTTGAG 1 cut(s) 355
Bsa29I ATCGAT 1 cut(s) 567
BsaJI CCNNGG 1 cut(s) 163
BsaWI WCCGGW 1 cut(s) 419
BsaXI ACNNNNNCTCC 2 cut(s) 88, 118
Bsc4I CCNNNNNNNGG 3 cut(s) 98, 113, 236
Bse118I RCCGGY 1 cut(s) 419
Bse1I ACTGG 1 cut(s) 103
BseBI CCWGG 1 cut(s) 191
BseCI ATCGAT 1 cut(s) 567
BseDI CCNNGG 1 cut(s) 163
BseGI GGATG 1 cut(s) 538
BseLI CCNNNNNNNGG 3 cut(s) 98, 113, 236
BseNI ACTGG 1 cut(s) 103
BseRI GAGGAG 1 cut(s) 533
BseXI GCAGC 3 cut(s) 244, 310, 338
BseYI CCCAGC 1 cut(s) 206
BshFI GGCC 1 cut(s) 343
BshTI ACCGGT 1 cut(s) 419
BshVI ATCGAT 1 cut(s) 567
BsiHKCI CYCGRG 1 cut(s) 501
BsiSI CCGG 1 cut(s) 420
BslFI GGGAC 1 cut(s) 433
BslI CCNNNNNNNGG 3 cut(s) 98, 113, 236
BsmAI GTCTC 2 cut(s) 30, 515
BsmFI GGGAC 1 cut(s) 433
BsnI GGCC 1 cut(s) 343
BsoBI CYCGRG 1 cut(s) 501
Bsp143I GATC 2 cut(s) 129, 148
BspANI GGCC 1 cut(s) 343
BspDI ATCGAT 1 cut(s) 567
BspMAI CTGCAG 1 cut(s) 328
BspPI GGATC 1 cut(s) 143
BsrFI RCCGGY 1 cut(s) 419
BsrI ACTGG 1 cut(s) 103
BssAI RCCGGY 1 cut(s) 419
BssECI CCNNGG 1 cut(s) 163
BssMI GATC 2 cut(s) 129, 148
BssT1I CCWWGG 1 cut(s) 163
Bst2UI CCWGG 1 cut(s) 191
Bst4CI ACNGT 4 cut(s) 13, 34, 202, 349
BstC8I GCNNGC 1 cut(s) 93
BstDEI CTNAG 1 cut(s) 414
BstF5I GGATG 1 cut(s) 538
BstKTI GATC 2 cut(s) 132, 151
BstMAI GTCTC 2 cut(s) 30, 515
BstMBI GATC 2 cut(s) 129, 148
BstMWI GCNNNNNNNGC 2 cut(s) 320, 497
BstNI CCWGG 1 cut(s) 191
BstNSI RCATGY 1 cut(s) 584
BstSCI CCNGG 1 cut(s) 189
BstSFI CTRYAG 3 cut(s) 198, 324, 345
BstV1I GCAGC 3 cut(s) 244, 310, 338
BstV2I GAAGAC 1 cut(s) 416
BstX2I RGATCY 1 cut(s) 148
BstYI RGATCY 1 cut(s) 148
Bsu15I ATCGAT 1 cut(s) 567
BsuI GTATCC 1 cut(s) 150
BsuRI GGCC 1 cut(s) 343
BsuTUI ATCGAT 1 cut(s) 567
BtgZI GCGATG 2 cut(s) 368, 444
BtsCI GGATG 1 cut(s) 538
BtsIMutI CAGTG 1 cut(s) 39
Cac8I GCNNGC 1 cut(s) 93
CaiI CAGNNNCTG 2 cut(s) 98, 206
Cfr10I RCCGGY 1 cut(s) 419
ClaI ATCGAT 1 cut(s) 567
CseI GACGC 1 cut(s) 464
CspAI ACCGGT 1 cut(s) 419
CviAII CATG 1 cut(s) 581
CviJI RGCY 6 cut(s) 91, 257, 323, 329, 343, 491
CviKI_1 RGCY 6 cut(s) 91, 257, 323, 329, 343, 491
DdeI CTNAG 1 cut(s) 414
DpnI GATC 2 cut(s) 131, 150
DpnII GATC 2 cut(s) 129, 148
Eco130I CCWWGG 1 cut(s) 163
Eco57I CTGAAG 1 cut(s) 129
Eco88I CYCGRG 1 cut(s) 501
EcoRII CCWGG 1 cut(s) 189
EcoT14I CCWWGG 1 cut(s) 163
ErhI CCWWGG 1 cut(s) 163
FaeI CATG 1 cut(s) 584
FaiI YATR 7 cut(s) 62, 110, 232, 287, 340, 549, 582
FaqI GGGAC 1 cut(s) 433
FatI CATG 1 cut(s) 580
Fnu4HI GCNGC 3 cut(s) 258, 324, 327
FokI GGATG 1 cut(s) 545
Fsp4HI GCNGC 3 cut(s) 258, 324, 327
FspBI CTAG 2 cut(s) 276, 320
GluI GCNGC 3 cut(s) 258, 324, 327
GsaI CCCAGC 1 cut(s) 210
HaeIII GGCC 1 cut(s) 343
HapII CCGG 1 cut(s) 420
HgaI GACGC 1 cut(s) 464
Hin1II CATG 1 cut(s) 584
HpaII CCGG 1 cut(s) 420
HphI GGTGA 1 cut(s) 554
Hpy188I TCNGA 4 cut(s) 30, 68, 148, 269
Hpy188III TCNNGA 1 cut(s) 372
HpyAV CCTTC 1 cut(s) 153
HpyCH4III ACNGT 4 cut(s) 13, 34, 202, 349
HpyCH4V TGCA 3 cut(s) 135, 326, 563
HpyF10VI GCNNNNNNNGC 2 cut(s) 320, 497
HpyF3I CTNAG 1 cut(s) 414
Hsp92II CATG 1 cut(s) 584
Kzo9I GATC 2 cut(s) 129, 148
LpnPI CCDG 9 cut(s) 84, 105, 176, 192, 203, 300, 433, 494, 539
Lsp1109I GCAGC 3 cut(s) 244, 310, 338
LweI GCATC 1 cut(s) 467
MaeI CTAG 2 cut(s) 276, 320
MaeIII GTNAC 2 cut(s) 7, 349
MalI GATC 2 cut(s) 131, 150
MboI GATC 2 cut(s) 129, 148
MboII GAAGA 1 cut(s) 416
MflI RGATCY 1 cut(s) 148
MnlI CCTC 4 cut(s) 197, 302, 499, 511
MseI TTAA 1 cut(s) 591
MspI CCGG 1 cut(s) 420
MspR9I CCNGG 1 cut(s) 191
MvaI CCWGG 1 cut(s) 191
MwoI GCNNNNNNNGC 2 cut(s) 320, 497
NdeII GATC 2 cut(s) 129, 148
NlaIII CATG 1 cut(s) 584
NmuCI GTSAC 1 cut(s) 7
NspI RCATGY 1 cut(s) 584
PflMI CCANNNNNTGG 1 cut(s) 98
PinAI ACCGGT 1 cut(s) 419
PkrI GCNGC 3 cut(s) 259, 325, 328
Psp6I CCWGG 1 cut(s) 189
PspFI CCCAGC 1 cut(s) 206
PspGI CCWGG 1 cut(s) 189
PstI CTGCAG 1 cut(s) 328
PstNI CAGNNNCTG 2 cut(s) 98, 206
PsuI RGATCY 1 cut(s) 148
SaqAI TTAA 1 cut(s) 591
SatI GCNGC 3 cut(s) 258, 324, 327
Sau3AI GATC 2 cut(s) 129, 148
ScrFI CCNGG 1 cut(s) 191
SetI ASST 6 cut(s) 259, 325, 331, 381, 493, 558
SfaNI GCATC 1 cut(s) 467
SfcI CTRYAG 3 cut(s) 198, 324, 345
SmlI CTYRAG 1 cut(s) 370
SmoI CTYRAG 1 cut(s) 370
SpeI ACTAGT 1 cut(s) 275
SspI AATATT 1 cut(s) 589
SspMI CTAG 2 cut(s) 276, 320
StyD4I CCNGG 1 cut(s) 189
StyI CCWWGG 1 cut(s) 163
TaaI ACNGT 4 cut(s) 13, 34, 202, 349
TaqI TCGA 2 cut(s) 567, 573
Tru1I TTAA 1 cut(s) 591
Tru9I TTAA 1 cut(s) 591
TscAI CASTG 1 cut(s) 39
TseFI GTSAC 1 cut(s) 7
TseI GCWGC 3 cut(s) 257, 323, 326
Tsp45I GTSAC 1 cut(s) 7
TspDTI ATGAA 3 cut(s) 49, 353, 371
TspRI CASTG 1 cut(s) 39
Van91I CCANNNNNTGG 1 cut(s) 98
XceI RCATGY 1 cut(s) 584
XspI CTAG 2 cut(s) 276, 320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.