AT4G31130

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0002236
Physical Location & Seq
Reverse (-)
15136909 .. 15138871
1963 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G31130.1

Sequence Viewer

Length: 588 bp
ATGGCGGTTAGTATGAAACAAATGTCGTTGGTCGTCTCTGCTTTGGGCGTCTTATCCTTTGTTTTGGGAGTTATCGCCGAGAACAAGAAGCCTGCGTCTGGGACTCCAATAAGTGGAAAAGGAGTAGTTATCTGCAAATACCCATCTGATCCAACTGTTGCCTTGGGATACCTTTCTGCTGCTTTTCTCCTTGCCTGCACTGTAGCAGGATACAAATCCTTGTTCATCTCTTACAAGGGAAAGTCTGTTCCTAACTCTGTCTTGTTCAAAAGCACCAGCTTCTCTGTGTTCTTCAACATTGCCTTGATAACATCTGGACTGGCCTTGTCTCTGTTGCTCTGGCCAACCATTACAGAACAACTTCACTTGACCCGTAATGTTCACCGAAACTTAGAAACCAGTTGCCCCACGGCTAAGACCGGTCTGCTTGGTGGAGGTGCCTTTGTGTCTTTGGACTCGTGCCTTTTCTGGTTGGTTGCTCTGATGCTCGCTGATAACGCCCGTGAAGACCATTTTGATGAAGTTGAAAGCAGAAGCATCGATGGGAACTCTTCCTCCAGGGATGTGAATCTCAAGATTGATGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

195

Amino Acids

20.75

Weight (kDa)

8.35

Isoelectric Point (pI)

39.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 60 - 154 4.1e-17 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 437
AccB7I CCANNNNNTGG 1 cut(s) 113
AciI CCGC 1 cut(s) 5
AclWI GGATC 1 cut(s) 143
AcoI YGGCCR 1 cut(s) 341
AcyI GRCGYC 1 cut(s) 48
AfiI CCNNNNNNNGG 2 cut(s) 98, 113
AgeI ACCGGT 1 cut(s) 419
AgsI TTSAA 3 cut(s) 268, 295, 527
AjnI CCWGG 1 cut(s) 557
AloI GAACNNNNNNTCC 4 cut(s) 231, 263, 539, 571
AluBI AGCT 1 cut(s) 279
AluI AGCT 1 cut(s) 279
Alw26I GTCTC 2 cut(s) 40, 333
AlwI GGATC 1 cut(s) 143
AoxI GGCC 2 cut(s) 321, 341
ApeKI GCWGC 1 cut(s) 179
AsiGI ACCGGT 1 cut(s) 419
Asp700I GAANNNNTTC 1 cut(s) 360
AsuHPI GGTGA 1 cut(s) 374
BalI TGGCCA 1 cut(s) 343
BanI GGYRCC 1 cut(s) 437
BauI CACGAG 1 cut(s) 457
BbsI GAAGAC 1 cut(s) 513
BbvI GCAGC 1 cut(s) 166
BccI CCATC 2 cut(s) 151, 536
BceAI ACGGC 1 cut(s) 426
BcgI CGANNNNNNTGC 2 cut(s) 520, 554
BciT130I CCWGG 1 cut(s) 559
BciVI GTATCC 2 cut(s) 161, 203
BcoDI GTCTC 2 cut(s) 40, 333
BfmI CTRYAG 1 cut(s) 201
BfuI GTATCC 2 cut(s) 161, 203
BisI GCNGC 1 cut(s) 180
BlsI GCNGC 1 cut(s) 181
Bme1390I CCNGG 1 cut(s) 559
BmiI GGNNCC 1 cut(s) 439
BmrFI CCNGG 1 cut(s) 559
BmsI GCATC 3 cut(s) 474, 546, 571
BpiI GAAGAC 1 cut(s) 513
BpmI CTGGAG 1 cut(s) 541
BpuEI CTTGAG 1 cut(s) 557
Bsa29I ATCGAT 1 cut(s) 540
BsaBI GATNNNNATC 2 cut(s) 214, 567
BsaHI GRCGYC 1 cut(s) 48
BsaJI CCNNGG 3 cut(s) 162, 408, 558
BsaWI WCCGGW 1 cut(s) 419
BsaXI ACNNNNNCTCC 2 cut(s) 539, 569
Bsc4I CCNNNNNNNGG 2 cut(s) 98, 113
Bse118I RCCGGY 1 cut(s) 419
Bse1I ACTGG 2 cut(s) 324, 399
Bse3DI GCAATG 1 cut(s) 297
Bse8I GATNNNNATC 2 cut(s) 214, 567
BseBI CCWGG 1 cut(s) 559
BseCI ATCGAT 1 cut(s) 540
BseDI CCNNGG 3 cut(s) 162, 408, 558
BseGI GGATG 1 cut(s) 568
BseJI GATNNNNATC 2 cut(s) 214, 567
BseLI CCNNNNNNNGG 2 cut(s) 98, 113
BseMI GCAATG 1 cut(s) 297
BseNI ACTGG 2 cut(s) 324, 399
BseXI GCAGC 1 cut(s) 166
BsgI GTGCAG 1 cut(s) 181
BshFI GGCC 2 cut(s) 323, 343
BshNI GGYRCC 1 cut(s) 437
BshTI ACCGGT 1 cut(s) 419
BshVI ATCGAT 1 cut(s) 540
BsiSI CCGG 1 cut(s) 420
BslFI GGGAC 1 cut(s) 115
BslI CCNNNNNNNGG 2 cut(s) 98, 113
BsmAI GTCTC 2 cut(s) 40, 333
BsmBI CGTCTC 1 cut(s) 40
BsmFI GGGAC 1 cut(s) 115
BsnI GGCC 2 cut(s) 323, 343
Bsp143I GATC 1 cut(s) 148
BspACI CCGC 1 cut(s) 5
BspANI GGCC 2 cut(s) 323, 343
BspDI ATCGAT 1 cut(s) 540
BspLI GGNNCC 1 cut(s) 439
BspPI GGATC 1 cut(s) 143
BspT107I GGYRCC 1 cut(s) 437
BsrDI GCAATG 1 cut(s) 297
BsrFI RCCGGY 1 cut(s) 419
BsrI ACTGG 2 cut(s) 324, 399
BssAI RCCGGY 1 cut(s) 419
BssECI CCNNGG 3 cut(s) 162, 408, 558
BssMI GATC 1 cut(s) 148
BssNI GRCGYC 1 cut(s) 48
BssSI CACGAG 1 cut(s) 457
BssT1I CCWWGG 1 cut(s) 162
Bst2BI CACGAG 1 cut(s) 457
Bst2UI CCWGG 1 cut(s) 559
Bst4CI ACNGT 2 cut(s) 157, 202
Bst6I CTCTTC 1 cut(s) 556
BstACI GRCGYC 1 cut(s) 48
BstC8I GCNNGC 3 cut(s) 93, 196, 489
BstDEI CTNAG 2 cut(s) 391, 414
BstDSI CCRYGG 1 cut(s) 408
BstF5I GGATG 1 cut(s) 568
BstKTI GATC 1 cut(s) 151
BstMAI GTCTC 2 cut(s) 40, 333
BstMBI GATC 1 cut(s) 148
BstMWI GCNNNNNNNGC 1 cut(s) 497
BstNI CCWGG 1 cut(s) 559
BstSCI CCNGG 1 cut(s) 557
BstSFI CTRYAG 1 cut(s) 201
BstV1I GCAGC 1 cut(s) 166
BstV2I GAAGAC 1 cut(s) 513
Bsu15I ATCGAT 1 cut(s) 540
BsuI GTATCC 2 cut(s) 161, 203
BsuRI GGCC 2 cut(s) 323, 343
BsuTUI ATCGAT 1 cut(s) 540
BtgI CCRYGG 1 cut(s) 408
BtsCI GGATG 1 cut(s) 568
BtsIMutI CAGTG 1 cut(s) 198
Cac8I GCNNGC 3 cut(s) 93, 196, 489
Cfr10I RCCGGY 1 cut(s) 419
ClaI ATCGAT 1 cut(s) 540
CseI GACGC 2 cut(s) 37, 84
CspAI ACCGGT 1 cut(s) 419
CviJI RGCY 5 cut(s) 91, 279, 323, 343, 413
CviKI_1 RGCY 5 cut(s) 91, 279, 323, 343, 413
DdeI CTNAG 2 cut(s) 391, 414
DpnI GATC 1 cut(s) 150
DpnII GATC 1 cut(s) 148
EaeI YGGCCR 1 cut(s) 341
Eam1104I CTCTTC 1 cut(s) 556
EarI CTCTTC 1 cut(s) 556
Eco130I CCWWGG 1 cut(s) 162
EcoRII CCWGG 1 cut(s) 557
EcoT14I CCWWGG 1 cut(s) 162
ErhI CCWWGG 1 cut(s) 162
Esp3I CGTCTC 1 cut(s) 40
FaiI YATR 1 cut(s) 14
FaqI GGGAC 1 cut(s) 115
Fnu4HI GCNGC 1 cut(s) 180
FokI GGATG 1 cut(s) 575
Fsp4HI GCNGC 1 cut(s) 180
GluI GCNGC 1 cut(s) 180
GsuI CTGGAG 1 cut(s) 541
HaeIII GGCC 2 cut(s) 323, 343
HapII CCGG 1 cut(s) 420
HgaI GACGC 2 cut(s) 37, 84
Hin1I GRCGYC 1 cut(s) 48
HinfI GANTC 3 cut(s) 103, 455, 568
HpaII CCGG 1 cut(s) 420
HphI GGTGA 1 cut(s) 374
Hpy166II GTNNAC 1 cut(s) 382
Hpy188I TCNGA 2 cut(s) 148, 483
Hpy188III TCNNGA 2 cut(s) 315, 574
Hpy8I GTNNAC 1 cut(s) 382
HpyCH4III ACNGT 2 cut(s) 157, 202
HpyCH4V TGCA 2 cut(s) 135, 198
HpyF10VI GCNNNNNNNGC 1 cut(s) 497
HpyF3I CTNAG 2 cut(s) 391, 414
Hsp92I GRCGYC 1 cut(s) 48
Kzo9I GATC 1 cut(s) 148
Lsp1109I GCAGC 1 cut(s) 166
LweI GCATC 3 cut(s) 474, 546, 571
MalI GATC 1 cut(s) 150
MboI GATC 1 cut(s) 148
MboII GAAGA 3 cut(s) 283, 518, 543
MlsI TGGCCA 1 cut(s) 343
MluNI TGGCCA 1 cut(s) 343
MlyI GAGTC 2 cut(s) 97, 449
MmeI TCCRAC 1 cut(s) 176
MnlI CCTC 2 cut(s) 428, 565
Mox20I TGGCCA 1 cut(s) 343
MroXI GAANNNNTTC 1 cut(s) 360
MscI TGGCCA 1 cut(s) 343
MslI CAYNNNNRTG 1 cut(s) 516
Msp20I TGGCCA 1 cut(s) 343
MspI CCGG 1 cut(s) 420
MspR9I CCNGG 1 cut(s) 559
MvaI CCWGG 1 cut(s) 559
MwoI GCNNNNNNNGC 1 cut(s) 497
NdeII GATC 1 cut(s) 148
NlaIV GGNNCC 1 cut(s) 439
NmeAIII GCCGAG 1 cut(s) 103
PdmI GAANNNNTTC 1 cut(s) 360
PfeI GAWTC 1 cut(s) 568
PflMI CCANNNNNTGG 1 cut(s) 113
PinAI ACCGGT 1 cut(s) 419
PkrI GCNGC 1 cut(s) 181
PleI GAGTC 2 cut(s) 97, 449
PpsI GAGTC 2 cut(s) 97, 449
Psp6I CCWGG 1 cut(s) 557
PspGI CCWGG 1 cut(s) 557
PspN4I GGNNCC 1 cut(s) 439
RseI CAYNNNNRTG 1 cut(s) 516
SatI GCNGC 1 cut(s) 180
Sau3AI GATC 1 cut(s) 148
SchI GAGTC 2 cut(s) 97, 449
ScrFI CCNGG 1 cut(s) 559
SetI ASST 3 cut(s) 174, 281, 439
SfaNI GCATC 3 cut(s) 474, 546, 571
SfcI CTRYAG 1 cut(s) 201
SmiMI CAYNNNNRTG 1 cut(s) 516
SmlI CTYRAG 1 cut(s) 572
SmoI CTYRAG 1 cut(s) 572
SsiI CCGC 1 cut(s) 5
StyD4I CCNGG 1 cut(s) 557
StyI CCWWGG 1 cut(s) 162
TaaI ACNGT 2 cut(s) 157, 202
TaqI TCGA 1 cut(s) 540
TfiI GAWTC 1 cut(s) 568
TscAI CASTG 1 cut(s) 205
TseI GCWGC 1 cut(s) 179
TspDTI ATGAA 3 cut(s) 29, 214, 534
TspRI CASTG 1 cut(s) 205
Van91I CCANNNNNTGG 1 cut(s) 113
XmnI GAANNNNTTC 1 cut(s) 360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.