Rh6AG512800

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0002236
Physical Location & Seq
Reverse (-)
68284543 .. 68287377
2835 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG512800.1

Sequence Viewer

Length: 606 bp
ATGGCTGTGACGGTGAAGCAGATGGCTATGATAGTGGCCTTTTTTGGTGTACTGTCTTTCATCTTCGGGATTATTGCGGAAAACAAGAAGCCGGCAGCTGGAAAACCAGTGCCGTGGAAAGATGTTGTTATTTGCAAGTATCCTTCTGATCCTTCGGTGGCGTTGGGATATCTGTCATTTGCATTTCTCCTTGTATCTACAGTGGTTGGGTTCTTGTCACTGTTTTACCCATATCAAGGGAAGTCCCTTCCGCAAGCTGCTTTGTTCCGGAGCAAGAGTTTCGTCGTCTTCTTCAACATTTCTTTGCTTACGGCAGGACTAGCTGCAGCTCTGTTGCTGTGGACTACAATAACGGAGCAACTTCATCTATCACGAAATGTCCATCACAATCTTAAAACAGACTGCCCCACTGCTAAGACTGGTCTCCTTGGTGGCGGTGCATTTGTATCCCTTGATTCAGCCCTTTTCTGGTTGGTTGCCCTCATGCTGGCCGACAATGCCCGGGAGGACTACTTTGAGGAGTCGGATAAGGATGTGAAGGGTAGTGGGTATACAGAGGTACACGGAGCTGATTATGGACAACATGTTGTAAAAGGTAGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

201

Amino Acids

21.76

Weight (kDa)

7.77

Isoelectric Point (pI)

30.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 59 - 155 9.3e-19 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 551
AccIII TCCGGA 1 cut(s) 267
AciI CCGC 3 cut(s) 77, 251, 435
AclWI GGATC 1 cut(s) 143
AcoI YGGCCR 1 cut(s) 489
AfaI GTAC 2 cut(s) 51, 561
AfiI CCNNNNNNNGG 4 cut(s) 98, 236, 468, 487
AflIII ACRYGT 1 cut(s) 583
AgsI TTSAA 1 cut(s) 295
AluBI AGCT 5 cut(s) 98, 257, 323, 329, 569
AluI AGCT 5 cut(s) 98, 257, 323, 329, 569
Alw26I GTCTC 1 cut(s) 428
AlwI GGATC 1 cut(s) 143
Ama87I CYCGRG 1 cut(s) 501
Aor13HI TCCGGA 1 cut(s) 267
AoxI GGCC 2 cut(s) 36, 489
ApeKI GCWGC 4 cut(s) 95, 257, 323, 326
AsuC2I CCSGG 2 cut(s) 502, 503
AsuHPI GGTGA 1 cut(s) 25
AvaI CYCGRG 1 cut(s) 501
BbsI GAAGAC 1 cut(s) 280
BbvI GCAGC 4 cut(s) 107, 244, 310, 338
BccI CCATC 2 cut(s) 16, 390
BceAI ACGGC 2 cut(s) 97, 327
BcgI CGANNNNNNTGC 2 cut(s) 262, 296
BciVI GTATCC 2 cut(s) 150, 457
BcnI CCSGG 2 cut(s) 502, 503
BcoDI GTCTC 1 cut(s) 428
BfaI CTAG 1 cut(s) 320
BfmI CTRYAG 2 cut(s) 198, 324
BfuI GTATCC 2 cut(s) 150, 457
BisI GCNGC 4 cut(s) 96, 258, 324, 327
BlsI GCNGC 4 cut(s) 97, 259, 325, 328
Bme1390I CCNGG 2 cut(s) 502, 503
BmeT110I CYCGRG 1 cut(s) 501
BmrFI CCNGG 2 cut(s) 502, 503
BpiI GAAGAC 1 cut(s) 280
BpuMI CCSGG 2 cut(s) 502, 503
BsaI GGTCTC 1 cut(s) 428
BsaJI CCNNGG 3 cut(s) 113, 427, 501
BsaWI WCCGGW 1 cut(s) 267
BsaXI ACNNNNNCTCC 2 cut(s) 262, 292
Bsc4I CCNNNNNNNGG 4 cut(s) 98, 236, 468, 487
Bse118I RCCGGY 1 cut(s) 91
Bse1I ACTGG 2 cut(s) 107, 424
BseAI TCCGGA 1 cut(s) 267
BseDI CCNNGG 3 cut(s) 113, 427, 501
BseGI GGATG 1 cut(s) 538
BseLI CCNNNNNNNGG 4 cut(s) 98, 236, 468, 487
BseNI ACTGG 2 cut(s) 107, 424
BseRI GAGGAG 1 cut(s) 533
BseXI GCAGC 4 cut(s) 107, 244, 310, 338
BshFI GGCC 2 cut(s) 38, 491
BsiHKCI CYCGRG 1 cut(s) 501
BsiSI CCGG 3 cut(s) 92, 268, 502
BslFI GGGAC 1 cut(s) 229
BslI CCNNNNNNNGG 4 cut(s) 98, 236, 468, 487
BsmAI GTCTC 1 cut(s) 428
BsmFI GGGAC 1 cut(s) 229
BsnI GGCC 2 cut(s) 38, 491
Bso31I GGTCTC 1 cut(s) 428
BsoBI CYCGRG 1 cut(s) 501
Bsp13I TCCGGA 1 cut(s) 267
Bsp143I GATC 1 cut(s) 148
BspACI CCGC 3 cut(s) 77, 251, 435
BspANI GGCC 2 cut(s) 38, 491
BspEI TCCGGA 1 cut(s) 267
BspMAI CTGCAG 1 cut(s) 328
BspPI GGATC 1 cut(s) 143
BspTNI GGTCTC 1 cut(s) 428
BsrFI RCCGGY 1 cut(s) 91
BsrI ACTGG 2 cut(s) 107, 424
BssAI RCCGGY 1 cut(s) 91
BssECI CCNNGG 3 cut(s) 113, 427, 501
BssMI GATC 1 cut(s) 148
BssNAI GTATAC 1 cut(s) 552
BssT1I CCWWGG 1 cut(s) 427
Bst1107I GTATAC 1 cut(s) 552
Bst4CI ACNGT 4 cut(s) 13, 54, 202, 222
BstC8I GCNNGC 3 cut(s) 93, 255, 489
BstDEI CTNAG 1 cut(s) 414
BstDSI CCRYGG 1 cut(s) 113
BstF5I GGATG 1 cut(s) 538
BstKTI GATC 1 cut(s) 151
BstMAI GTCTC 1 cut(s) 428
BstMBI GATC 1 cut(s) 148
BstMWI GCNNNNNNNGC 2 cut(s) 320, 497
BstNSI RCATGY 1 cut(s) 587
BstSCI CCNGG 2 cut(s) 500, 501
BstSFI CTRYAG 2 cut(s) 198, 324
BstV1I GCAGC 4 cut(s) 107, 244, 310, 338
BstV2I GAAGAC 1 cut(s) 280
BstXI CCANNNNNNTGG 1 cut(s) 114
BstZ17I GTATAC 1 cut(s) 552
BsuI GTATCC 2 cut(s) 150, 457
BsuRI GGCC 2 cut(s) 38, 491
BtgI CCRYGG 1 cut(s) 113
BtsCI GGATG 1 cut(s) 538
BtsI GCAGTG 1 cut(s) 408
BtsIMutI CAGTG 4 cut(s) 114, 207, 218, 408
Cac8I GCNNGC 3 cut(s) 93, 255, 489
Cfr10I RCCGGY 1 cut(s) 91
Cfr9I CCCGGG 1 cut(s) 501
Csp6I GTAC 2 cut(s) 50, 560
CviAII CATG 2 cut(s) 484, 584
CviQI GTAC 2 cut(s) 50, 560
DdeI CTNAG 1 cut(s) 414
DpnI GATC 1 cut(s) 150
DpnII GATC 1 cut(s) 148
EaeI YGGCCR 1 cut(s) 489
Eco130I CCWWGG 1 cut(s) 427
Eco31I GGTCTC 1 cut(s) 428
Eco32I GATATC 1 cut(s) 170
Eco88I CYCGRG 1 cut(s) 501
EcoRV GATATC 1 cut(s) 170
EcoT14I CCWWGG 1 cut(s) 427
ErhI CCWWGG 1 cut(s) 427
FaeI CATG 2 cut(s) 487, 587
FaiI YATR 6 cut(s) 29, 232, 485, 552, 576, 585
FaqI GGGAC 1 cut(s) 229
FatI CATG 2 cut(s) 483, 583
FblI GTMKAC 1 cut(s) 551
Fnu4HI GCNGC 4 cut(s) 96, 258, 324, 327
FokI GGATG 1 cut(s) 545
Fsp4HI GCNGC 4 cut(s) 96, 258, 324, 327
FspBI CTAG 1 cut(s) 320
GluI GCNGC 4 cut(s) 96, 258, 324, 327
HaeIII GGCC 2 cut(s) 38, 491
HapII CCGG 3 cut(s) 92, 268, 502
Hin1II CATG 2 cut(s) 487, 587
HinfI GANTC 2 cut(s) 455, 521
HpaII CCGG 3 cut(s) 92, 268, 502
HphI GGTGA 1 cut(s) 25
Hpy166II GTNNAC 4 cut(s) 50, 342, 552, 562
Hpy188I TCNGA 2 cut(s) 148, 526
Hpy188III TCNNGA 3 cut(s) 67, 268, 372
Hpy8I GTNNAC 4 cut(s) 50, 342, 552, 562
Hpy99I CGWCG 1 cut(s) 287
HpyAV CCTTC 4 cut(s) 153, 162, 257, 532
HpyCH4III ACNGT 4 cut(s) 13, 54, 202, 222
HpyCH4V TGCA 4 cut(s) 135, 182, 326, 440
HpyF10VI GCNNNNNNNGC 2 cut(s) 320, 497
HpyF3I CTNAG 1 cut(s) 414
Hsp92II CATG 2 cut(s) 487, 587
Kpn2I TCCGGA 1 cut(s) 267
KroI GCCGGC 1 cut(s) 91
KroNI GCCGGC 1 cut(s) 93
Kzo9I GATC 1 cut(s) 148
LmnI GCTCC 3 cut(s) 270, 355, 566
LpnPI CCDG 9 cut(s) 84, 105, 120, 281, 300, 405, 454, 473, 515
Lsp1109I GCAGC 4 cut(s) 107, 244, 310, 338
MaeI CTAG 1 cut(s) 320
MaeIII GTNAC 2 cut(s) 7, 216
MalI GATC 1 cut(s) 150
MboI GATC 1 cut(s) 148
MboII GAAGA 3 cut(s) 55, 280, 283
MlyI GAGTC 1 cut(s) 530
MmeI TCCRAC 1 cut(s) 504
MnlI CCTC 4 cut(s) 491, 499, 511, 550
MroI TCCGGA 1 cut(s) 267
MroNI GCCGGC 1 cut(s) 91
MseI TTAA 1 cut(s) 393
MspA1I CMGCKG 1 cut(s) 98
MspI CCGG 3 cut(s) 92, 268, 502
MspR9I CCNGG 2 cut(s) 502, 503
MwoI GCNNNNNNNGC 2 cut(s) 320, 497
NaeI GCCGGC 1 cut(s) 93
NciI CCSGG 2 cut(s) 502, 503
NdeII GATC 1 cut(s) 148
NgoMIV GCCGGC 1 cut(s) 91
NlaIII CATG 2 cut(s) 487, 587
NmuCI GTSAC 2 cut(s) 7, 216
NspI RCATGY 1 cut(s) 587
PciI ACATGT 1 cut(s) 583
PdiI GCCGGC 1 cut(s) 93
PfeI GAWTC 1 cut(s) 455
PkrI GCNGC 4 cut(s) 97, 259, 325, 328
PleI GAGTC 1 cut(s) 529
PpsI GAGTC 1 cut(s) 529
PscI ACATGT 1 cut(s) 583
PstI CTGCAG 1 cut(s) 328
PvuII CAGCTG 1 cut(s) 98
RsaI GTAC 2 cut(s) 51, 561
RsaNI GTAC 2 cut(s) 50, 560
SaqAI TTAA 1 cut(s) 393
SatI GCNGC 4 cut(s) 96, 258, 324, 327
Sau3AI GATC 1 cut(s) 148
SchI GAGTC 1 cut(s) 530
ScrFI CCNGG 2 cut(s) 502, 503
SetI ASST 7 cut(s) 100, 259, 325, 331, 561, 571, 598
SfcI CTRYAG 2 cut(s) 198, 324
SmaI CCCGGG 1 cut(s) 503
SsiI CCGC 3 cut(s) 77, 251, 435
SspMI CTAG 1 cut(s) 320
StyD4I CCNGG 2 cut(s) 500, 501
StyI CCWWGG 1 cut(s) 427
TaaI ACNGT 4 cut(s) 13, 54, 202, 222
TatI WGTACW 1 cut(s) 49
TfiI GAWTC 1 cut(s) 455
Tru1I TTAA 1 cut(s) 393
Tru9I TTAA 1 cut(s) 393
TscAI CASTG 4 cut(s) 114, 207, 225, 415
TseFI GTSAC 2 cut(s) 7, 216
TseI GCWGC 4 cut(s) 95, 257, 323, 326
Tsp45I GTSAC 2 cut(s) 7, 216
TspDTI ATGAA 2 cut(s) 49, 353
TspGWI ACGGA 2 cut(s) 368, 579
TspMI CCCGGG 1 cut(s) 501
TspRI CASTG 4 cut(s) 114, 207, 225, 415
XceI RCATGY 1 cut(s) 587
XmaI CCCGGG 1 cut(s) 501
XmiI GTMKAC 1 cut(s) 551
XspI CTAG 1 cut(s) 320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.