Rh6BG523700

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0002236
Physical Location & Seq
Reverse (-)
71598873 .. 71601653
2781 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG523700.1

Sequence Viewer

Length: 549 bp
ATGACTGGATACTTTGTTTTGACTTATTGGCAGCCGGCAGCTGGAAAACCAGTGCCGTGGAAAGATGTTGTTATTTGCAAGTATCCTTCTGATCCTTCGGTGGCGTTGGGATATCTGTCATTTGCATTTCTCCTTATATCTACAGTGGTTGGGTTCTTGTCACTGTTTTACCCATATCAAGGGAAGTCCCTTCCGCAAGCTGCTTTGTTCCGGAGCAAGAGTTTCGTTGTCTTCTTCAACATTTCTTTGCTTACAGCAGGACTAGCTGCAGCTCTGTTGCTGTGGACTACAATAACGGAGCAACTTCATCTATCACGAAATGTCCATCACAATCTTAAAACAGACTGCCCCACTGCTAAGACCGGTCTCCTTGGTGGCGGTGCTTTTGTATCCCTTGATTCATCCCTTTTCTGGTTGGTTGCCCTCATGCTGGCCGACAATGCCCGAGAGGACTACTTTGAGGAGTCGGATAAGGATGTCAAGGGTAGTGGGTATACAGACGTACATGGAGCTGATTATGGACAACATGTTGTAAAAGGTAGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

182

Amino Acids

19.88

Weight (kDa)

6.49

Isoelectric Point (pI)

30.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 40 - 135 8.8e-19 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 494
AccIII TCCGGA 1 cut(s) 210
AciI CCGC 2 cut(s) 194, 378
AclWI GGATC 1 cut(s) 86
AcoI YGGCCR 1 cut(s) 432
AfaI GTAC 1 cut(s) 504
AfiI CCNNNNNNNGG 4 cut(s) 41, 179, 411, 430
AflIII ACRYGT 1 cut(s) 526
AgeI ACCGGT 1 cut(s) 362
AgsI TTSAA 1 cut(s) 238
AluBI AGCT 5 cut(s) 41, 200, 266, 272, 512
AluI AGCT 5 cut(s) 41, 200, 266, 272, 512
Alw26I GTCTC 1 cut(s) 371
AlwI GGATC 1 cut(s) 86
Ama87I CYCGRG 1 cut(s) 444
Aor13HI TCCGGA 1 cut(s) 210
AoxI GGCC 1 cut(s) 432
ApeKI GCWGC 5 cut(s) 31, 38, 200, 266, 269
AsiGI ACCGGT 1 cut(s) 362
AvaI CYCGRG 1 cut(s) 444
BbsI GAAGAC 1 cut(s) 223
BbvI GCAGC 5 cut(s) 43, 50, 187, 253, 281
BccI CCATC 1 cut(s) 333
BceAI ACGGC 1 cut(s) 40
BcgI CGANNNNNNTGC 2 cut(s) 205, 239
BciVI GTATCC 2 cut(s) 93, 400
BcoDI GTCTC 1 cut(s) 371
BfaI CTAG 1 cut(s) 263
BfmI CTRYAG 2 cut(s) 141, 267
BfuI GTATCC 2 cut(s) 93, 400
BisI GCNGC 5 cut(s) 32, 39, 201, 267, 270
BlsI GCNGC 5 cut(s) 33, 40, 202, 268, 271
BmeT110I CYCGRG 1 cut(s) 444
BpiI GAAGAC 1 cut(s) 223
BsaI GGTCTC 1 cut(s) 371
BsaJI CCNNGG 2 cut(s) 56, 370
BsaWI WCCGGW 2 cut(s) 210, 362
BsaXI ACNNNNNCTCC 2 cut(s) 205, 235
Bsc4I CCNNNNNNNGG 4 cut(s) 41, 179, 411, 430
Bse118I RCCGGY 2 cut(s) 34, 362
Bse1I ACTGG 2 cut(s) 10, 50
BseAI TCCGGA 1 cut(s) 210
BseDI CCNNGG 2 cut(s) 56, 370
BseGI GGATG 2 cut(s) 401, 481
BseLI CCNNNNNNNGG 4 cut(s) 41, 179, 411, 430
BseNI ACTGG 2 cut(s) 10, 50
BseRI GAGGAG 1 cut(s) 476
BseXI GCAGC 5 cut(s) 43, 50, 187, 253, 281
BshFI GGCC 1 cut(s) 434
BshTI ACCGGT 1 cut(s) 362
BsiHKCI CYCGRG 1 cut(s) 444
BsiSI CCGG 3 cut(s) 35, 211, 363
BslFI GGGAC 1 cut(s) 172
BslI CCNNNNNNNGG 4 cut(s) 41, 179, 411, 430
BsmAI GTCTC 1 cut(s) 371
BsmFI GGGAC 1 cut(s) 172
BsnI GGCC 1 cut(s) 434
Bso31I GGTCTC 1 cut(s) 371
BsoBI CYCGRG 1 cut(s) 444
Bsp13I TCCGGA 1 cut(s) 210
Bsp143I GATC 1 cut(s) 91
BspACI CCGC 2 cut(s) 194, 378
BspANI GGCC 1 cut(s) 434
BspEI TCCGGA 1 cut(s) 210
BspMAI CTGCAG 1 cut(s) 271
BspPI GGATC 1 cut(s) 86
BspTNI GGTCTC 1 cut(s) 371
BsrFI RCCGGY 2 cut(s) 34, 362
BsrI ACTGG 2 cut(s) 10, 50
BssAI RCCGGY 2 cut(s) 34, 362
BssECI CCNNGG 2 cut(s) 56, 370
BssMI GATC 1 cut(s) 91
BssNAI GTATAC 1 cut(s) 495
BssT1I CCWWGG 1 cut(s) 370
Bst1107I GTATAC 1 cut(s) 495
Bst4CI ACNGT 2 cut(s) 145, 165
BstC8I GCNNGC 3 cut(s) 36, 198, 432
BstDEI CTNAG 1 cut(s) 357
BstDSI CCRYGG 1 cut(s) 56
BstF5I GGATG 2 cut(s) 401, 481
BstKTI GATC 1 cut(s) 94
BstMAI GTCTC 1 cut(s) 371
BstMBI GATC 1 cut(s) 91
BstMWI GCNNNNNNNGC 2 cut(s) 263, 440
BstNSI RCATGY 1 cut(s) 530
BstSFI CTRYAG 2 cut(s) 141, 267
BstV1I GCAGC 5 cut(s) 43, 50, 187, 253, 281
BstV2I GAAGAC 1 cut(s) 223
BstXI CCANNNNNNTGG 1 cut(s) 57
BstZ17I GTATAC 1 cut(s) 495
BsuI GTATCC 2 cut(s) 93, 400
BsuRI GGCC 1 cut(s) 434
BtgI CCRYGG 1 cut(s) 56
BtsCI GGATG 2 cut(s) 401, 481
BtsI GCAGTG 1 cut(s) 351
BtsIMutI CAGTG 4 cut(s) 57, 150, 161, 351
Cac8I GCNNGC 3 cut(s) 36, 198, 432
Cfr10I RCCGGY 2 cut(s) 34, 362
Csp6I GTAC 1 cut(s) 503
CspAI ACCGGT 1 cut(s) 362
CspCI CAANNNNNGTGG 2 cut(s) 469, 504
CviAII CATG 3 cut(s) 427, 506, 527
CviJI RGCY 7 cut(s) 34, 41, 200, 266, 272, 434, 512
CviKI_1 RGCY 7 cut(s) 34, 41, 200, 266, 272, 434, 512
CviQI GTAC 1 cut(s) 503
DdeI CTNAG 1 cut(s) 357
DpnI GATC 1 cut(s) 93
DpnII GATC 1 cut(s) 91
EaeI YGGCCR 1 cut(s) 432
Eco130I CCWWGG 1 cut(s) 370
Eco31I GGTCTC 1 cut(s) 371
Eco32I GATATC 1 cut(s) 113
Eco88I CYCGRG 1 cut(s) 444
EcoRV GATATC 1 cut(s) 113
EcoT14I CCWWGG 1 cut(s) 370
ErhI CCWWGG 1 cut(s) 370
FaeI CATG 3 cut(s) 430, 509, 530
FaiI YATR 7 cut(s) 137, 175, 428, 495, 507, 519, 528
FaqI GGGAC 1 cut(s) 172
FatI CATG 3 cut(s) 426, 505, 526
FblI GTMKAC 1 cut(s) 494
Fnu4HI GCNGC 5 cut(s) 32, 39, 201, 267, 270
FokI GGATG 2 cut(s) 388, 488
Fsp4HI GCNGC 5 cut(s) 32, 39, 201, 267, 270
FspBI CTAG 1 cut(s) 263
GluI GCNGC 5 cut(s) 32, 39, 201, 267, 270
HaeIII GGCC 1 cut(s) 434
HapII CCGG 3 cut(s) 35, 211, 363
Hin1II CATG 3 cut(s) 430, 509, 530
HinfI GANTC 2 cut(s) 398, 464
HpaII CCGG 3 cut(s) 35, 211, 363
Hpy166II GTNNAC 2 cut(s) 285, 495
Hpy188I TCNGA 2 cut(s) 91, 469
Hpy188III TCNNGA 2 cut(s) 211, 315
Hpy8I GTNNAC 2 cut(s) 285, 495
HpyAV CCTTC 3 cut(s) 96, 105, 200
HpyCH4III ACNGT 2 cut(s) 145, 165
HpyCH4IV ACGT 1 cut(s) 501
HpyCH4V TGCA 3 cut(s) 78, 125, 269
HpyF10VI GCNNNNNNNGC 2 cut(s) 263, 440
HpyF3I CTNAG 1 cut(s) 357
HpySE526I ACGT 1 cut(s) 501
Hsp92II CATG 3 cut(s) 430, 509, 530
Kpn2I TCCGGA 1 cut(s) 210
KroI GCCGGC 1 cut(s) 34
KroNI GCCGGC 1 cut(s) 36
Kzo9I GATC 1 cut(s) 91
LmnI GCTCC 3 cut(s) 213, 298, 509
LpnPI CCDG 8 cut(s) 27, 48, 63, 224, 243, 376, 397, 416
Lsp1109I GCAGC 5 cut(s) 43, 50, 187, 253, 281
MaeI CTAG 1 cut(s) 263
MaeII ACGT 1 cut(s) 501
MaeIII GTNAC 1 cut(s) 159
MalI GATC 1 cut(s) 93
MboI GATC 1 cut(s) 91
MboII GAAGA 2 cut(s) 223, 226
MlyI GAGTC 1 cut(s) 473
MmeI TCCRAC 1 cut(s) 447
MnlI CCTC 3 cut(s) 434, 442, 454
MroI TCCGGA 1 cut(s) 210
MroNI GCCGGC 1 cut(s) 34
MseI TTAA 1 cut(s) 336
MspA1I CMGCKG 1 cut(s) 41
MspI CCGG 3 cut(s) 35, 211, 363
MwoI GCNNNNNNNGC 2 cut(s) 263, 440
NaeI GCCGGC 1 cut(s) 36
NdeII GATC 1 cut(s) 91
NgoMIV GCCGGC 1 cut(s) 34
NlaIII CATG 3 cut(s) 430, 509, 530
NmuCI GTSAC 1 cut(s) 159
NspI RCATGY 1 cut(s) 530
PciI ACATGT 1 cut(s) 526
PdiI GCCGGC 1 cut(s) 36
PfeI GAWTC 1 cut(s) 398
PinAI ACCGGT 1 cut(s) 362
PkrI GCNGC 5 cut(s) 33, 40, 202, 268, 271
PleI GAGTC 1 cut(s) 472
PpsI GAGTC 1 cut(s) 472
PscI ACATGT 1 cut(s) 526
PstI CTGCAG 1 cut(s) 271
PvuII CAGCTG 1 cut(s) 41
RsaI GTAC 1 cut(s) 504
RsaNI GTAC 1 cut(s) 503
SaqAI TTAA 1 cut(s) 336
SatI GCNGC 5 cut(s) 32, 39, 201, 267, 270
Sau3AI GATC 1 cut(s) 91
SchI GAGTC 1 cut(s) 473
SetI ASST 7 cut(s) 43, 202, 268, 274, 504, 514, 541
SfcI CTRYAG 2 cut(s) 141, 267
SsiI CCGC 2 cut(s) 194, 378
SspMI CTAG 1 cut(s) 263
StyI CCWWGG 1 cut(s) 370
TaaI ACNGT 2 cut(s) 145, 165
TaiI ACGT 1 cut(s) 504
TfiI GAWTC 1 cut(s) 398
Tru1I TTAA 1 cut(s) 336
Tru9I TTAA 1 cut(s) 336
TscAI CASTG 4 cut(s) 57, 150, 168, 358
TseFI GTSAC 1 cut(s) 159
TseI GCWGC 5 cut(s) 31, 38, 200, 266, 269
Tsp45I GTSAC 1 cut(s) 159
TspDTI ATGAA 2 cut(s) 296, 390
TspGWI ACGGA 1 cut(s) 311
TspRI CASTG 4 cut(s) 57, 150, 168, 358
XceI RCATGY 1 cut(s) 530
XmiI GTMKAC 1 cut(s) 494
XspI CTAG 1 cut(s) 263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.