Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0008122

Physical Location & Seq

Genomic Coordinates Reverse (-)

14481928 .. 14486861

Gene Structure 4934 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1145800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2703 bp

ATGGATTCACCTGAAATTCCCAAAATCATAGCCAGCACATCACCTTCCTCAGATTCTCCTCCAGTTCAAGAGTCACCAGTTTTCAGTTACATTAGCAATCTATCACCAATACAGCCTGTGAAGGCTTCACATGCAGCGCAAGGGTTCCCGGGACTTAGCTCTCCTCCTCTTGTGTTCACGTCACCACGCATAAATACACTCCGGGAAACAAGTTTCTTGAAAAGGCCTCAATACCCACAATTATCCAGTGCAGAAAAACCTAAAGCACAAGATGAAGCCAAGAAATTTGTTGATGGTCCAGTTGATTCCAAGAAACATATTACCCAACTGCAGATGGGATCGATTACTGATTCTCAGGACTGTGAGACCAACAGTTCCTCAGAGGGTGTTGATGAATACTTAGCCGATCCCATGGAGATGGACTGTACGAATTCTGCTCAATCAGTCAATCCATGTTTGAAAGAATCTAAAATTTCCTATAAATCTGAGGCACCATCAGAGCAGGCTACAGAGGACCTTCAAGGAAAGCAAACATTTGATGCAAAACCTGTCAAAATTAAAGAGCTTAGTCATGGTGCGTTGCCATCTTCCGAGCGCCCAAAGGTTGGATCCGGAATATCTGTCGATAATGCTTTCAATGGAGAGTATCACCATGGTTTGCATAATCAGGGCTTCGGAGGTCAACACCAGGATGATTGTGATCATAGCCCTCAATCTCCTCCTGGACGTTTGCAGATTGGTCAGGTGTATGAGGATTGTACAGAGAACGTCGGTGGAACCTCTAAGGGAATAATCGGGAACATGATATTGCATGCTCCTAATAAGGTCAAAAATGACCAAGGTGGCATGCATAGACGTTGTCTTCAGTTTGAAGAGGCTCCTCCTTGTGCCACTGGAAAAAGAGACAGTTCTTCTATAGAGAAAGTTAACGATTCAGAACCACCTGCCAGCACTGCAGAATTGGAGATTGTGAAAGTATCTTATGCAGCAACTTCCAAGAGACAGATGGGTGCCTCATTGCCACCTCTGTATGGTGGAAGCTCTCCTTTAACTGTGCCCAAACCATCAGGTATTGGCTTGCACCTAAACAGCATAGTCAATGCTGTACCCTTTGTTCGTGGTGCAACAACAAGCATAAAATTAGCAGATCACGACATTGGTTTGCAAGTTATGAAATCATCAGCTGTTGTGAGCCACCATTTATCGGAGAATGTGAGGGATGACACTGAAACTTCTTCAATTGCTGCAAGTTCCTCCATCACCCAGTCTCCCCACACTGTGGAATTTGAGCATCTTGGATCTCCCCTTGAGGAGAGGAAATCTGATTCTCAGAATGTTGACAGTTATAAGGAGTTGAACCAATCTAGTTCACAAAAGAAAAGGAAAAGAACACCAAGCTCTAAAGATAGTGATGGGTGCAAGCGGTGCAACTGTAAGAAGACCAAGTGCTTGAAACTTTATTGCGATTGTTTTGCTGCTGGAGTTTATTGTGCCGAAACTTGTGCTTGCCAAGGATGCTTTAATATACCCGATTATGAAGATACAGTTCTTGAGACGAGGCAACAAATTGAAGCCCGTAATCCACTTGCATTTGCCCCCAAGATTGTACAGCTTGAAGAAGAAATTCAGTTTACACCAGCCTCGGCAAGACACAAAAGGGGGTGCAATTGCAAAAAGTCAATGTGTCTGAAAAAATATTGTGAATGCTATCAGGCCAATGTTGGATGCTCCAGTGGATGTCGCTGTGATGGATGTAAAAATGTTTATGGCAGGAAGGGAGATTATGGTCCAATAGATCATGGTGTGACTAAAGACATGATTAGTGACAAAGCCGGCAAGGAAACATTTGATGAGAAGCTGGAAATGGTGGCAACAAAGAAAGATGCTTTGTCTACTGAGTTGTATGATTCACACAACCACACTCCATTGACGCCATCATTTCAGTGCTCAGATCCTGCAGATAATGTACCAAAATCCCCTTGCCTTCTTACAAGTTACCTTCGATCACCTGAATCTGATCTTACCGTCATATCATCTTATGAAAAATCAACAAGATCCTCTCTGAGAAATTCAGAGACCAGTGGGATTCTTCTGGAGACAAGTAAAGAATTATCAGGTATGGGTTATTACTGCTGGCAGGAGGACTATGACAACATTGGAATAGCAGACACTTTTTCACCAAGATACGACGCTGCCCCCATTATATGCCATATTACTCCATTATCAGATCTTTGCTCAAAGGCCTCAGCTTCTTCCACATCATCAAGAAGTGATTGGAAAAATGCTTCACAAGTTCAGCTATGCCCTGAAAGTCAAGGTCTGTCATCCAATAGTTCTCTTCGTTGGTGTAGTTCACCACTCACCCCAATGACTCGGTTAGGTGGGACGGAATGTTCTCAAGGTCTGGACTTTGACCATGGGCTTTCTGACATTATGCAAGAAGAAATGCCTGAGATACTTAAGGACATTTCTGCTACCAATAAGTCGGTGAAAGTAAGTTCCCCCAATAAGAAACGGGTTTCTCCACCCCACAACCACAATCATGAGCTTGGTGCAAGCTCTTCAGGGAGCTTGAGAAGTGGCCGCAAGTTCATATTGAAGGCTGTCCCTTCGTTCCCACCCCTTACCCCTTGTAGCAGTTCCAAAGGTAGTAATACCATTCAGAGCACCGGTAATCTTCACGATAAGGGTAGGAAGAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04218 map04218

Pfam Domains

Protein Families

TCR

Protein Analysis

901

Amino Acids

97.61

Weight (kDa)

6.38

Isoelectric Point (pI)

62.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TCR	PF03638	474 - 508	3.6e-11	Tesmin/TSO1-like CXC domain, cysteine-rich domain
TCR	PF03638	552 - 587	3.6e-11	Tesmin/TSO1-like CXC domain, cysteine-rich domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008122)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g29570 FvH4_2g29570 FvH4_2g29570
malus_domestica	MD08G1145800.v1.1 MD15G1121200.v1.1
prunus_persica	Prupe.1G473800_v2.0.a1 Prupe.1G473800_v2.0.a1 Prupe.1G473800_v2.0.a1
pyrus_communis	pycom08g12270 pycom15g10940
rosa_chinensis	RchiOBHm_Chr6g0298531
rosa_multiflora	Rmu_co8315197.1_g000001 Rmu_co8448729.1_g000001 Rmu_sc0004500.1_g000005 Rmu_ssc0000289.1_g000056
rosa_roxburghii	Rroxscaffold_7G00169560
rosa_rugosa	Rorug06G0281000 Rorug06G0281000
rosa_samantha	Rh6AG392300 Rh6BG400100 Rh6CG406000 Rh6DG392400 Rh6DG392500
rosa_wichuraiana	Rw6G034290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1347
AarI	CACCTGC	1 cut(s)	952
Acc36I	ACCTGC	1 cut(s)	952
AccB1I	GGYRCC	2 cut(s)	490, 1010
AccB7I	CCANNNNNTGG	2 cut(s)	605, 1279
AccI	GTMKAC	1 cut(s)	1892
AccIII	TCCGGA	1 cut(s)	611
AciI	CCGC	2 cut(s)	1423, 2584
AclWI	GGATC	7 cut(s)	346, 401, 603, 616, 1306, 1946, 2049
AcoI	YGGCCR	1 cut(s)	2581
AcsI	RAATTY	7 cut(s)	15, 284, 430, 471, 1283, 1623, 2068
AcuI	CTGAAG	2 cut(s)	848, 2547
AcyI	GRCGYC	1 cut(s)	1931
AdeI	CACNNNGTG	1 cut(s)	1279
AfaI	GTAC	5 cut(s)	427, 760, 1107, 1608, 1968
AfiI	CCNNNNNNNGG	4 cut(s)	605, 1031, 1204, 1279
AflII	CTTAAG	1 cut(s)	2459
AgeI	ACCGGT	1 cut(s)	2669
AjiI	CACGTC	1 cut(s)	180
AjnI	CCWGG	2 cut(s)	687, 721
AloI	GAACNNNNNNTCC	2 cut(s)	2377, 2409
Alw21I	GWGCWC	2 cut(s)	1949, 2669
Alw26I	GTCTC	7 cut(s)	359, 897, 994, 1272, 1547, 2069, 2090
AlwI	GGATC	7 cut(s)	346, 401, 603, 616, 1306, 1946, 2049
AlwNI	CAGNNNCTG	1 cut(s)	1955
Ama87I	CYCGRG	1 cut(s)	148
Aor13HI	TCCGGA	1 cut(s)	611
AoxI	GGCC	4 cut(s)	224, 1713, 2241, 2581
ApeKI	GCWGC	5 cut(s)	134, 986, 1244, 1475, 2192
ApoI	RAATTY	7 cut(s)	15, 284, 430, 471, 1283, 1623, 2068
AsiGI	ACCGGT	1 cut(s)	2669
Asp700I	GAANNNNTTC	1 cut(s)	1623
AspLEI	GCGC	2 cut(s)	139, 597
AspS9I	GGNCC	3 cut(s)	296, 514, 1787
AsuC2I	CCSGG	3 cut(s)	149, 150, 203
AvaI	CYCGRG	1 cut(s)	148
AvaII	GGWCC	3 cut(s)	296, 514, 1787
BaeGI	GKGCMC	1 cut(s)	1059
BamHI	GGATCC	1 cut(s)	608
BanI	GGYRCC	2 cut(s)	490, 1010
BbsI	GAAGAC	2 cut(s)	854, 1445
Bbv12I	GWGCWC	2 cut(s)	1949, 2669
BbvCI	CCTCAGC	1 cut(s)	2245
BbvI	GCAGC	5 cut(s)	146, 998, 1231, 1462, 2179
BcgI	CGANNNNNNTGC	4 cut(s)	1454, 1484, 1488, 1518
BciT130I	CCWGG	2 cut(s)	689, 723
BclI	TGATCA	1 cut(s)	700
BcnI	CCSGG	3 cut(s)	149, 150, 203
BcoDI	GTCTC	7 cut(s)	359, 897, 994, 1272, 1547, 2069, 2090
BfaI	CTAG	1 cut(s)	1365
BfmI	CTRYAG	5 cut(s)	329, 507, 915, 954, 1956
BfoI	RGCGCY	1 cut(s)	598
BfrI	CTTAAG	1 cut(s)	2459
BfuAI	ACCTGC	1 cut(s)	952
BglII	AGATCT	1 cut(s)	2227
BisI	GCNGC	6 cut(s)	135, 987, 1245, 1476, 2193, 2584
BlsI	GCNGC	6 cut(s)	136, 988, 1246, 1477, 2194, 2585
Bme1390I	CCNGG	5 cut(s)	149, 150, 203, 689, 723
Bme18I	GGWCC	3 cut(s)	296, 514, 1787
BmeT110I	CYCGRG	1 cut(s)	148
BmgBI	CACGTC	1 cut(s)	180
BmgT120I	GGNCC	3 cut(s)	296, 514, 1787
BmiI	GGNNCC	6 cut(s)	146, 492, 610, 778, 879, 1012
BmrFI	CCNGG	5 cut(s)	149, 150, 203, 689, 723
BmrI	ACTGGG	1 cut(s)	1258
BmsI	GCATC	5 cut(s)	529, 1300, 1505, 1715, 1873
BmuI	ACTGGG	1 cut(s)	1258
BpiI	GAAGAC	2 cut(s)	854, 1445
BpmI	CTGGAG	4 cut(s)	45, 1500, 1714, 2114
Bpu10I	CCTNAGC	1 cut(s)	2245
BpuEI	CTTGAG	4 cut(s)	1328, 1571, 2382, 2593
BpuMI	CCSGG	3 cut(s)	149, 150, 203
Bsa29I	ATCGAT	1 cut(s)	341
BsaBI	GATNNNNATC	1 cut(s)	699
BsaHI	GRCGYC	1 cut(s)	1931
BsaI	GGTCTC	2 cut(s)	359, 2069
BsaJI	CCNNGG	7 cut(s)	148, 411, 652, 838, 1510, 1641, 2416
BsaWI	WCCGGW	2 cut(s)	611, 2669
BsaXI	ACNNNNNCTCC	2 cut(s)	1252, 1282
Bsc4I	CCNNNNNNNGG	4 cut(s)	605, 1031, 1204, 1279
Bse118I	RCCGGY	2 cut(s)	1832, 2669
Bse1I	ACTGG	8 cut(s)	62, 77, 246, 299, 898, 1264, 1731, 2079
Bse3DI	GCAATG	1 cut(s)	1016
Bse8I	GATNNNNATC	1 cut(s)	699
BseAI	TCCGGA	1 cut(s)	611
BseBI	CCWGG	2 cut(s)	689, 723
BseCI	ATCGAT	1 cut(s)	341
BseDI	CCNNGG	7 cut(s)	148, 411, 652, 838, 1510, 1641, 2416
BseGI	GGATG	7 cut(s)	697, 1225, 1520, 1730, 1742, 1757, 2324
BseJI	GATNNNNATC	1 cut(s)	699
BseLI	CCNNNNNNNGG	4 cut(s)	605, 1031, 1204, 1279
BseMI	GCAATG	1 cut(s)	1016
BseNI	ACTGG	8 cut(s)	62, 77, 246, 299, 898, 1264, 1731, 2079
BseRI	GAGGAG	6 cut(s)	48, 153, 156, 708, 870, 1325
BseSI	GKGCMC	1 cut(s)	1059
BseXI	GCAGC	5 cut(s)	146, 998, 1231, 1462, 2179
BsgI	GTGCAG	1 cut(s)	270
BshFI	GGCC	4 cut(s)	226, 1715, 2243, 2583
BshNI	GGYRCC	2 cut(s)	490, 1010
BshTI	ACCGGT	1 cut(s)	2669
BshVI	ATCGAT	1 cut(s)	341
BsiHKAI	GWGCWC	2 cut(s)	1949, 2669
BsiHKCI	CYCGRG	1 cut(s)	148
BsiSI	CCGG	5 cut(s)	149, 202, 612, 1833, 2670
BslFI	GGGAC	3 cut(s)	165, 2398, 2591
BslI	CCNNNNNNNGG	4 cut(s)	605, 1031, 1204, 1279
BsmAI	GTCTC	7 cut(s)	359, 897, 994, 1272, 1547, 2069, 2090
BsmBI	CGTCTC	1 cut(s)	1547
BsmFI	GGGAC	3 cut(s)	165, 2398, 2591
BsmI	GAATGC	1 cut(s)	1709
BsnI	GGCC	4 cut(s)	226, 1715, 2243, 2583
Bso31I	GGTCTC	2 cut(s)	359, 2069
BsoBI	CYCGRG	1 cut(s)	148
Bsp1286I	GDGCHC	3 cut(s)	1059, 1949, 2669
Bsp13I	TCCGGA	1 cut(s)	611
Bsp1407I	TGTACA	2 cut(s)	758, 1606
Bsp19I	CCATGG	3 cut(s)	411, 652, 2416
BspACI	CCGC	2 cut(s)	1423, 2584
BspANI	GGCC	4 cut(s)	226, 1715, 2243, 2583
BspDI	ATCGAT	1 cut(s)	341
BspEI	TCCGGA	1 cut(s)	611
BspHI	TCATGA	1 cut(s)	2542
BspLI	GGNNCC	6 cut(s)	146, 492, 610, 778, 879, 1012
BspMAI	CTGCAG	3 cut(s)	333, 958, 1960
BspMI	ACCTGC	1 cut(s)	952
BspPI	GGATC	7 cut(s)	346, 401, 603, 616, 1306, 1946, 2049
BspQI	GCTCTTC	1 cut(s)	2566
BspT107I	GGYRCC	2 cut(s)	490, 1010
BspTI	CTTAAG	1 cut(s)	2459
BspTNI	GGTCTC	2 cut(s)	359, 2069
BsrDI	GCAATG	1 cut(s)	1016
BsrFI	RCCGGY	2 cut(s)	1832, 2669
BsrGI	TGTACA	2 cut(s)	758, 1606
BsrI	ACTGG	8 cut(s)	62, 77, 246, 299, 898, 1264, 1731, 2079
BssAI	RCCGGY	2 cut(s)	1832, 2669
BssECI	CCNNGG	7 cut(s)	148, 411, 652, 838, 1510, 1641, 2416
BssNI	GRCGYC	1 cut(s)	1931
BssT1I	CCWWGG	5 cut(s)	411, 652, 838, 1510, 2416
Bst2UI	CCWGG	2 cut(s)	689, 723
Bst6I	CTCTTC	3 cut(s)	867, 2343, 2566
BstACI	GRCGYC	1 cut(s)	1931
BstAFI	CTTAAG	1 cut(s)	2459
BstAPI	GCANNNNNTGC	1 cut(s)	1425
BstAUI	TGTACA	2 cut(s)	758, 1606
BstDSI	CCRYGG	3 cut(s)	411, 652, 2416
BstF5I	GGATG	7 cut(s)	697, 1225, 1520, 1730, 1742, 1757, 2324
BstH2I	RGCGCY	1 cut(s)	598
BstHHI	GCGC	2 cut(s)	139, 597
BstMAI	GTCTC	7 cut(s)	359, 897, 994, 1272, 1547, 2069, 2090
BstMWI	GCNNNNNNNGC	4 cut(s)	131, 953, 1425, 1515
BstNI	CCWGG	2 cut(s)	689, 723
BstNSI	RCATGY	3 cut(s)	134, 815, 850
BstSCI	CCNGG	5 cut(s)	147, 148, 201, 687, 721
BstSFI	CTRYAG	5 cut(s)	329, 507, 915, 954, 1956
BstSLI	GKGCMC	1 cut(s)	1059
BstV1I	GCAGC	5 cut(s)	146, 998, 1231, 1462, 2179
BstV2I	GAAGAC	2 cut(s)	854, 1445
BstX2I	RGATCY	5 cut(s)	608, 1298, 1951, 2054, 2227
BstXI	CCANNNNNNTGG	1 cut(s)	418
BstYI	RGATCY	5 cut(s)	608, 1298, 1951, 2054, 2227
Bsu15I	ATCGAT	1 cut(s)	341
BsuRI	GGCC	4 cut(s)	226, 1715, 2243, 2583
BsuTUI	ATCGAT	1 cut(s)	341
BtgI	CCRYGG	3 cut(s)	411, 652, 2416
BtrI	CACGTC	1 cut(s)	180
BtsCI	GGATG	7 cut(s)	697, 1225, 1520, 1730, 1742, 1757, 2324
BtsI	GCAGTG	1 cut(s)	951
BtsIMutI	CAGTG	8 cut(s)	253, 891, 951, 1224, 1275, 1738, 1949, 2086
BveI	ACCTGC	1 cut(s)	952
CaiI	CAGNNNCTG	1 cut(s)	1955
CciI	TCATGA	1 cut(s)	2542
CfoI	GCGC	2 cut(s)	139, 597
Cfr10I	RCCGGY	2 cut(s)	1832, 2669
Cfr13I	GGNCC	3 cut(s)	296, 514, 1787
Cfr9I	CCCGGG	1 cut(s)	148
ClaI	ATCGAT	1 cut(s)	341
CseI	GACGC	2 cut(s)	1939, 2198
Csp6I	GTAC	5 cut(s)	426, 759, 1106, 1607, 1967
CspAI	ACCGGT	1 cut(s)	2669
CviQI	GTAC	5 cut(s)	426, 759, 1106, 1607, 1967
DraIII	CACNNNGTG	1 cut(s)	1279
EaeI	YGGCCR	1 cut(s)	2581
Eam1104I	CTCTTC	3 cut(s)	867, 2343, 2566
EarI	CTCTTC	3 cut(s)	867, 2343, 2566
Eco130I	CCWWGG	5 cut(s)	411, 652, 838, 1510, 2416
Eco147I	AGGCCT	2 cut(s)	226, 2243
Eco31I	GGTCTC	2 cut(s)	359, 2069
Eco47I	GGWCC	3 cut(s)	296, 514, 1787
Eco57I	CTGAAG	2 cut(s)	848, 2547
Eco88I	CYCGRG	1 cut(s)	148
EcoO109I	RGGNCCY	1 cut(s)	514
EcoRI	GAATTC	1 cut(s)	430
EcoRII	CCWGG	2 cut(s)	687, 721
EcoT14I	CCWWGG	5 cut(s)	411, 652, 838, 1510, 2416
EcoT22I	ATGCAT	1 cut(s)	852
ErhI	CCWWGG	5 cut(s)	411, 652, 838, 1510, 2416
Esp3I	CGTCTC	1 cut(s)	1547
FalI	AAGNNNNNCTT	2 cut(s)	1030, 1062
FaqI	GGGAC	3 cut(s)	165, 2398, 2591
FbaI	TGATCA	1 cut(s)	700
FblI	GTMKAC	1 cut(s)	1892
Fnu4HI	GCNGC	6 cut(s)	135, 987, 1245, 1476, 2193, 2584
FokI	GGATG	7 cut(s)	704, 1232, 1527, 1737, 1749, 1764, 2311
Fsp4HI	GCNGC	6 cut(s)	135, 987, 1245, 1476, 2193, 2584
FspBI	CTAG	1 cut(s)	1365
GlaI	GCGC	2 cut(s)	138, 596
GluI	GCNGC	6 cut(s)	135, 987, 1245, 1476, 2193, 2584
GsuI	CTGGAG	4 cut(s)	45, 1500, 1714, 2114
HaeII	RGCGCY	1 cut(s)	598
HaeIII	GGCC	4 cut(s)	226, 1715, 2243, 2583
HapII	CCGG	5 cut(s)	149, 202, 612, 1833, 2670
HgaI	GACGC	2 cut(s)	1939, 2198
HhaI	GCGC	2 cut(s)	139, 597
Hin1I	GRCGYC	1 cut(s)	1931
Hin6I	GCGC	2 cut(s)	137, 595
HinP1I	GCGC	2 cut(s)	137, 595
HincII	GTYRAC	3 cut(s)	683, 928, 1339
HindII	GTYRAC	3 cut(s)	683, 928, 1339
HpaI	GTTAAC	1 cut(s)	928
HpaII	CCGG	5 cut(s)	149, 202, 612, 1833, 2670
Hpy166II	GTNNAC	8 cut(s)	177, 683, 928, 1339, 1370, 1632, 1893, 2354
Hpy8I	GTNNAC	8 cut(s)	177, 683, 928, 1339, 1370, 1632, 1893, 2354
Hpy99I	CGWCG	2 cut(s)	773, 2192
HpyAV	CCTTC	8 cut(s)	54, 115, 527, 1768, 1994, 2009, 2593, 2619
HpyCH4IV	ACGT	4 cut(s)	179, 727, 768, 856
HpyF10VI	GCNNNNNNNGC	4 cut(s)	131, 953, 1425, 1515
HpySE526I	ACGT	4 cut(s)	179, 727, 768, 856
Hsp92I	GRCGYC	1 cut(s)	1931
HspAI	GCGC	2 cut(s)	137, 595
Kpn2I	TCCGGA	1 cut(s)	611
KroI	GCCGGC	1 cut(s)	1832
KroNI	GCCGGC	1 cut(s)	1834
Ksp22I	TGATCA	1 cut(s)	700
KspAI	GTTAAC	1 cut(s)	928
LguI	GCTCTTC	1 cut(s)	2566
LmnI	GCTCC	4 cut(s)	820, 883, 1733, 2568
Lsp1109I	GCAGC	5 cut(s)	146, 998, 1231, 1462, 2179
LweI	GCATC	5 cut(s)	529, 1300, 1505, 1715, 1873
MaeI	CTAG	1 cut(s)	1365
MaeII	ACGT	4 cut(s)	179, 727, 768, 856
MaeIII	GTNAC	6 cut(s)	72, 86, 180, 1804, 1823, 1994
MfeI	CAATTG	2 cut(s)	1239, 1666
MflI	RGATCY	5 cut(s)	608, 1298, 1951, 2054, 2227
MhlI	GDGCHC	3 cut(s)	1059, 1949, 2669
MlyI	GAGTC	2 cut(s)	80, 2365
MmeI	TCCRAC	2 cut(s)	586, 1702
Mph1103I	ATGCAT	1 cut(s)	852
MroI	TCCGGA	1 cut(s)	611
MroNI	GCCGGC	1 cut(s)	1832
MroXI	GAANNNNTTC	1 cut(s)	1623
MseI	TTAA	5 cut(s)	558, 927, 1049, 1521, 2460
MslI	CAYNNNNRTG	5 cut(s)	416, 690, 1942, 2366, 2541
MspA1I	CMGCKG	1 cut(s)	1184
MspCI	CTTAAG	1 cut(s)	2459
MspI	CCGG	5 cut(s)	149, 202, 612, 1833, 2670
MspR9I	CCNGG	5 cut(s)	149, 150, 203, 689, 723
MunI	CAATTG	2 cut(s)	1239, 1666
Mva1269I	GAATGC	1 cut(s)	1709
MvaI	CCWGG	2 cut(s)	689, 723
MwoI	GCNNNNNNNGC	4 cut(s)	131, 953, 1425, 1515
NaeI	GCCGGC	1 cut(s)	1834
NciI	CCSGG	3 cut(s)	149, 150, 203
NcoI	CCATGG	3 cut(s)	411, 652, 2416
NgoMIV	GCCGGC	1 cut(s)	1832
NlaIV	GGNNCC	6 cut(s)	146, 492, 610, 778, 879, 1012
NmeAIII	GCCGAG	1 cut(s)	1622
NmuCI	GTSAC	4 cut(s)	72, 180, 1804, 1823
NsiI	ATGCAT	1 cut(s)	852
NspI	RCATGY	3 cut(s)	134, 815, 850
PaeI	GCATGC	2 cut(s)	815, 850
PagI	TCATGA	1 cut(s)	2542
PaqCI	CACCTGC	1 cut(s)	952
PceI	AGGCCT	2 cut(s)	226, 2243
PciSI	GCTCTTC	1 cut(s)	2566
PctI	GAATGC	1 cut(s)	1709
PdiI	GCCGGC	1 cut(s)	1834
PdmI	GAANNNNTTC	1 cut(s)	1623
PflFI	GACNNNGTC	1 cut(s)	858
PflMI	CCANNNNNTGG	2 cut(s)	605, 1279
PfoI	TCCNGGA	2 cut(s)	201, 721
PinAI	ACCGGT	1 cut(s)	2669
PkrI	GCNGC	6 cut(s)	136, 988, 1246, 1477, 2194, 2585
PleI	GAGTC	2 cut(s)	79, 2365
PpsI	GAGTC	2 cut(s)	79, 2365
PpuMI	RGGWCCY	1 cut(s)	514
PsiI	TTATAA	1 cut(s)	1347
Psp5II	RGGWCCY	1 cut(s)	514
Psp6I	CCWGG	2 cut(s)	687, 721
PspGI	CCWGG	2 cut(s)	687, 721
PspN4I	GGNNCC	6 cut(s)	146, 492, 610, 778, 879, 1012
PspPI	GGNCC	3 cut(s)	296, 514, 1787
PspPPI	RGGWCCY	1 cut(s)	514
PsrI	GAACNNNNNNTAC	2 cut(s)	1098, 1130
PstI	CTGCAG	3 cut(s)	333, 958, 1960
PstNI	CAGNNNCTG	1 cut(s)	1955
PsuI	RGATCY	5 cut(s)	608, 1298, 1951, 2054, 2227
PsyI	GACNNNGTC	1 cut(s)	858
PvuII	CAGCTG	1 cut(s)	1184
RsaI	GTAC	5 cut(s)	427, 760, 1107, 1608, 1968
RsaNI	GTAC	5 cut(s)	426, 759, 1106, 1607, 1967
RseI	CAYNNNNRTG	5 cut(s)	416, 690, 1942, 2366, 2541
SapI	GCTCTTC	1 cut(s)	2566
SaqAI	TTAA	5 cut(s)	558, 927, 1049, 1521, 2460
SatI	GCNGC	6 cut(s)	135, 987, 1245, 1476, 2193, 2584
Sau96I	GGNCC	3 cut(s)	296, 514, 1787
SchI	GAGTC	2 cut(s)	80, 2365
ScrFI	CCNGG	5 cut(s)	149, 150, 203, 689, 723
SduI	GDGCHC	3 cut(s)	1059, 1949, 2669
SfaNI	GCATC	5 cut(s)	529, 1300, 1505, 1715, 1873
SfcI	CTRYAG	5 cut(s)	329, 507, 915, 954, 1956
SinI	GGWCC	3 cut(s)	296, 514, 1787
SmaI	CCCGGG	1 cut(s)	150
SmiMI	CAYNNNNRTG	5 cut(s)	416, 690, 1942, 2366, 2541
SmlI	CTYRAG	5 cut(s)	1307, 1550, 2397, 2459, 2572
SmoI	CTYRAG	5 cut(s)	1307, 1550, 2397, 2459, 2572
SphI	GCATGC	2 cut(s)	815, 850
SseBI	AGGCCT	2 cut(s)	226, 2243
SsiI	CCGC	2 cut(s)	1423, 2584
SspI	AATATT	1 cut(s)	1697
SspMI	CTAG	1 cut(s)	1365
StuI	AGGCCT	2 cut(s)	226, 2243
StyD4I	CCNGG	5 cut(s)	147, 148, 201, 687, 721
StyI	CCWWGG	5 cut(s)	411, 652, 838, 1510, 2416
TaiI	ACGT	4 cut(s)	182, 730, 771, 859
TaqI	TCGA	3 cut(s)	341, 624, 2002
TatI	WGTACW	2 cut(s)	758, 1606
TauI	GCSGC	1 cut(s)	2586
Tru1I	TTAA	5 cut(s)	558, 927, 1049, 1521, 2460
Tru9I	TTAA	5 cut(s)	558, 927, 1049, 1521, 2460
TscAI	CASTG	8 cut(s)	253, 898, 958, 1231, 1282, 1738, 1949, 2086
TseFI	GTSAC	4 cut(s)	72, 180, 1804, 1823
TseI	GCWGC	5 cut(s)	134, 986, 1244, 1475, 2192
Tsp45I	GTSAC	4 cut(s)	72, 180, 1804, 1823
TspDTI	ATGAA	6 cut(s)	288, 408, 1187, 1551, 2055, 2581
TspGWI	ACGGA	1 cut(s)	2402
TspMI	CCCGGG	1 cut(s)	148
TspRI	CASTG	8 cut(s)	253, 898, 958, 1231, 1282, 1738, 1949, 2086
Tth111I	GACNNNGTC	1 cut(s)	858
Van91I	CCANNNNNTGG	2 cut(s)	605, 1279
Vha464I	CTTAAG	1 cut(s)	2459
VpaK11BI	GGWCC	3 cut(s)	296, 514, 1787
XapI	RAATTY	7 cut(s)	15, 284, 430, 471, 1283, 1623, 2068
XceI	RCATGY	3 cut(s)	134, 815, 850
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1003
XmaI	CCCGGG	1 cut(s)	148
XmiI	GTMKAC	1 cut(s)	1892
XmnI	GAANNNNTTC	1 cut(s)	1623
XspI	CTAG	1 cut(s)	1365
Zsp2I	ATGCAT	1 cut(s)	852

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.