Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0008122

Physical Location & Seq

Genomic Coordinates Reverse (-)

8704899 .. 8709933

Gene Structure 5035 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1121200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2694 bp

ATGGATTCACCTGAAATTCCCAAAATCAAAGACAACACTTCACCTTCCTCAGATTCTCCTCCAGTTCAAGAGTCACCAGTCTTCAGTTACATAAGCAATTTATCACCAATACAGCCTGTGAAGGCTTCACATGCAGTGCAAGGGTTCCCGGGACTTAGCTCTCCTCCTCTTGTGTTCACGTCACCACGCATAAATACACTCCGGGAAACAAGTTTCCTGAAAAGGCCTCAATACCCGCGATTATCCAGTGTAGAAAAAACGAAATCACATGATGAAGCCAGGAAGTTTGTTGATGGTACAGTTGATTCCAGGAAACATATCACCCAACTGCAGATGGGATTGATTACTGATACTCAGGACTGTGAGACCAACAGTTCCTCGGAGGGTGTCGATGAATACTTAGCTGATCCCATGGAGATGGACAGTACGAATTCTTGTCAATCAGTCAATCCATGTTTGAAAGAATCTATCAATGCATCTGAATCTGAGGCACCACCAGAGAAGGCTAAAGAGGACCTTGACGGAAAGCAATTTGATGCAAAACCTGTGAAAAATAAAGAGCATAGTGATGGTGAGTTGCCATCTTCTGAGTGCCCAAAGGTTGGATCCGGCTTATCCATCGATAATGCTTTCAATGGGGAGTATCACCAAGGTTTTCATAATCAGGGTATCGGAGGTAGACACCAGGATGATTGTGATCATAACCCTCCATCTCCTCCTGGACGTTTGCAGATTGGTCAGGTGTATGAGGATTGTACAGAGAAGGTTGGTGGAACCTCAAAGGGAATGATTGGAAACATGATATTGCATGCTCCTAATAAGGCCAAAAATGACCAAGGTGGCATGCATAGACGTTGCCTTCAGTTTGAAGAGGCACCTCCTTGTGCGACTGGAAAAGGAGACAGCTCTTCTGTAGAGAAAGTTAACAATTCAGAACCACCTGCCAGCACAGCAGAATTGGAGATTGTGCAAGTACCTTATGCAGCAACTTCCAAGAGACAGATGGGAGCCTCATTGCCACCTCGGTATGGTGGAAGCTCTCCTTTAACTGTTCCCAAACCATCAGGTATTGGCTTGCATCTAAATAGCATAGTCAATGCTGCACCCGTTGTTCGTGGTGCAACAACCATAAAATTAGCAGATCACTACATTGGTGTGCAAGTTATGAAATCATCATCTGTTGTGAACCACCATTTACCTGAGAATGTGAGGGATGAAACTGAGAATTCTATTGCCACAAGCTCCTCCATCACCCAGTCTCCCCATACTGTGGAATTTGAGCGTCATGGATCTCCACCCGAGAAGAGCAAATTAGATTCTCAGAATCTTGATAGTTATCTGGAGTTGAACCAATCTAGCTCACAAAAGAAAAGGAAAAAAACACCAAGCTCTAAAGATAGTGACGGGTGCAAGCGCTGCAACTGTAAGAAGTCCAAGTGCTTGAAACTTTATTGCGATTGTTTTGCTGCTGGAGTCTATTGTGCCGAAACTTGTGCTTGCCAAGGATGCTTTAATATACCCGATTATGAAGATACGGTTCTTGAGACGAGACAACAAATTGAAGCCCGTAATCCACTTGCATTTGCTCCTAAGATTGTACAGCTAGAAGAAGAAATTCAATTTACACCAGCCTCAGCAAGACACAAAAGGGGATGCAATTGCAAAAAGTCAATGTGTCTGAAAAAATATTGTGAATGCTATCAGGCCAATGTTGGATGCTCCAGTGGATGCCGGTGTGATGGATGTAAAAATGTTTATGGCAGAAAGGGAGATTATGTTCCAATAGAACATGGTGTGCTTAAAGACACGATTAGTGACAAAGCCGGTAAGGAAACATTTGATGAGAAGCTGGAAATGGTGGCAACAAAGAAAGATATTTTGTCTACTGATTTGTATGATTCTCACAACCACACTCCATTGACGCCATCATTTCACTGCTCAGATCATGCAAATAATATACCAAAATCCCCGTGCCTTCCTGCAAGTTACCTTCGATCACCTGAATCTGATCTTACCATCATATCATCTTATGAAAAATCAACAAGATCCCCTCTGAGAAATTCAGAGACAGGTGGCATTCTTCTGGAGACAAGTAAAGAATTATCGGATATGGGTTATTACCACTGGCGAGAGGACTATGACAACATTGGAGTAGCGGACACTTTTTCAACAAGATACGACGCTGCCCCCACTACATGCCATATTACCCCATTATCAGATCTTTACTCAAAGGCCTCTGCTTCTTCCACATCATCAAGAAGTGATTGGAAAAATGCTTCACAAGCTCAGCTGTGTCCTGGAATTCAAGGTCTGTCATCTAGTAGTTCTCTTCATTGGCGTAGTTCACCAGTCACCCCAATGGCTCGGTTAGGTGGGACGAAATCTTCTCAGGGTCTTGACTTTGACAACGGGCTTTATGACATTCTGCAAGAAGAAACGCCTGAGATACTGAAGGACAGTTCTAATCCTATTAAGTCTGTGAAAGTTAGTTCCCCCAATAAGAAACGGGTTTCTCCTCCTCACAACCACAATCATGAGCTTGGTGCAAGCTCTTCAGGGAGCTTGAGAAGTGGCCGCAAGTTCATATTGAAGGCTGTCCCTTCGTTCCCACCCCTTACCCCTTGTAGCAATTCCGAAGGTAGTAATATCATTCAGAACACTAGTAATCTTCACGATAAGGGCAGAAAGAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04218 map04218

Pfam Domains

Protein Families

TCR

Protein Analysis

898

Amino Acids

97.76

Weight (kDa)

6.58

Isoelectric Point (pI)

62.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TCR	PF03638	471 - 505	2.2e-11	Tesmin/TSO1-like CXC domain, cysteine-rich domain
TCR	PF03638	549 - 584	3.6e-11	Tesmin/TSO1-like CXC domain, cysteine-rich domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008122)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g29570 FvH4_2g29570 FvH4_2g29570
malus_domestica	MD08G1145800.v1.1 MD15G1121200.v1.1
prunus_persica	Prupe.1G473800_v2.0.a1 Prupe.1G473800_v2.0.a1 Prupe.1G473800_v2.0.a1
pyrus_communis	pycom08g12270 pycom15g10940
rosa_chinensis	RchiOBHm_Chr6g0298531
rosa_multiflora	Rmu_co8315197.1_g000001 Rmu_co8448729.1_g000001 Rmu_sc0004500.1_g000005 Rmu_ssc0000289.1_g000056
rosa_roxburghii	Rroxscaffold_7G00169560
rosa_rugosa	Rorug06G0281000 Rorug06G0281000
rosa_samantha	Rh6AG392300 Rh6BG400100 Rh6CG406000 Rh6DG392400 Rh6DG392500
rosa_wichuraiana	Rw6G034290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	949
Acc36I	ACCTGC	1 cut(s)	949
AccB1I	GGYRCC	2 cut(s)	490, 874
AccB7I	CCANNNNNTGG	2 cut(s)	602, 1270
AccI	GTMKAC	2 cut(s)	679, 1883
AccII	CGCG	1 cut(s)	238
AciI	CCGC	3 cut(s)	236, 2156, 2575
AclWI	GGATC	5 cut(s)	401, 600, 613, 1297, 2040
AcoI	YGGCCR	1 cut(s)	2572
AcsI	RAATTY	7 cut(s)	15, 430, 1225, 1274, 1614, 2059, 2301
AcuI	CTGAAG	4 cut(s)	67, 845, 2471, 2538
AcyI	GRCGYC	1 cut(s)	1922
AfaI	GTAC	5 cut(s)	298, 427, 757, 975, 1599
AfeI	AGCGCT	1 cut(s)	1415
AfiI	CCNNNNNNNGG	3 cut(s)	602, 1028, 1270
AhlI	ACTAGT	1 cut(s)	2660
AjiI	CACGTC	1 cut(s)	180
AjnI	CCWGG	5 cut(s)	278, 308, 684, 718, 2296
AloI	GAACNNNNNNTCC	2 cut(s)	1761, 1793
Alw26I	GTCTC	8 cut(s)	359, 894, 991, 1263, 1538, 1543, 2060, 2081
AlwI	GGATC	5 cut(s)	401, 600, 613, 1297, 2040
Ama87I	CYCGRG	2 cut(s)	148, 1298
Aor51HI	AGCGCT	1 cut(s)	1415
AoxI	GGCC	5 cut(s)	224, 822, 1704, 2232, 2572
ApeKI	GCWGC	5 cut(s)	983, 1100, 1416, 1466, 2183
ApoI	RAATTY	7 cut(s)	15, 430, 1225, 1274, 1614, 2059, 2301
Asp700I	GAANNNNTTC	1 cut(s)	1614
AspLEI	GCGC	1 cut(s)	1416
AspS9I	GGNCC	1 cut(s)	514
AsuC2I	CCSGG	3 cut(s)	149, 150, 203
AvaI	CYCGRG	2 cut(s)	148, 1298
AvaII	GGWCC	1 cut(s)	514
BaeGI	GKGCMC	1 cut(s)	596
BamHI	GGATCC	1 cut(s)	605
BanI	GGYRCC	2 cut(s)	490, 874
BbsI	GAAGAC	1 cut(s)	73
BbvCI	CCTCAGC	1 cut(s)	1633
BbvI	GCAGC	5 cut(s)	995, 1087, 1403, 1453, 2170
BcgI	CGANNNNNNTGC	4 cut(s)	1445, 1475, 1479, 1509
BciT130I	CCWGG	5 cut(s)	280, 310, 686, 720, 2298
BclI	TGATCA	1 cut(s)	697
BcnI	CCSGG	3 cut(s)	149, 150, 203
BcoDI	GTCTC	8 cut(s)	359, 894, 991, 1263, 1538, 1543, 2060, 2081
BcuI	ACTAGT	1 cut(s)	2660
BfaI	CTAG	4 cut(s)	1356, 1604, 2319, 2661
BfmI	CTRYAG	2 cut(s)	329, 912
BfoI	RGCGCY	1 cut(s)	1417
BfuAI	ACCTGC	1 cut(s)	949
BglII	AGATCT	1 cut(s)	2218
BisI	GCNGC	6 cut(s)	984, 1101, 1417, 1467, 2184, 2575
BlpI	GCTNAGC	1 cut(s)	2286
BlsI	GCNGC	6 cut(s)	985, 1102, 1418, 1468, 2185, 2576
Bme1390I	CCNGG	8 cut(s)	149, 150, 203, 280, 310, 686, 720, 2298
Bme18I	GGWCC	1 cut(s)	514
BmeT110I	CYCGRG	2 cut(s)	148, 1298
BmgBI	CACGTC	1 cut(s)	180
BmgT120I	GGNCC	1 cut(s)	514
BmiI	GGNNCC	6 cut(s)	146, 492, 607, 775, 876, 1009
BmrFI	CCNGG	8 cut(s)	149, 150, 203, 280, 310, 686, 720, 2298
BmrI	ACTGGG	1 cut(s)	1249
BmsI	GCATC	7 cut(s)	485, 526, 1087, 1496, 1643, 1706, 1718
BmuI	ACTGGG	1 cut(s)	1249
BpiI	GAAGAC	1 cut(s)	73
BpmI	CTGGAG	5 cut(s)	45, 1361, 1491, 1705, 2105
Bpu10I	CCTNAGC	1 cut(s)	1633
Bpu1102I	GCTNAGC	1 cut(s)	2286
BpuEI	CTTGAG	2 cut(s)	1562, 2584
BpuMI	CCSGG	3 cut(s)	149, 150, 203
Bsa29I	ATCGAT	1 cut(s)	621
BsaBI	GATNNNNATC	2 cut(s)	696, 1335
BsaHI	GRCGYC	1 cut(s)	1922
BsaI	GGTCTC	1 cut(s)	359
BsaJI	CCNNGG	7 cut(s)	148, 378, 411, 649, 835, 1022, 1501
BsaXI	ACNNNNNCTCC	4 cut(s)	1243, 1273, 1761, 1791
Bsc4I	CCNNNNNNNGG	3 cut(s)	602, 1028, 1270
Bse118I	RCCGGY	2 cut(s)	1731, 1823
Bse1I	ACTGG	8 cut(s)	62, 77, 246, 895, 1255, 1722, 2129, 2348
Bse3DI	GCAATG	1 cut(s)	1013
Bse8I	GATNNNNATC	2 cut(s)	696, 1335
BseBI	CCWGG	5 cut(s)	280, 310, 686, 720, 2298
BseCI	ATCGAT	1 cut(s)	621
BseDI	CCNNGG	7 cut(s)	148, 378, 411, 649, 835, 1022, 1501
BseGI	GGATG	7 cut(s)	694, 1219, 1511, 1658, 1721, 1733, 1748
BseJI	GATNNNNATC	2 cut(s)	696, 1335
BseLI	CCNNNNNNNGG	3 cut(s)	602, 1028, 1270
BseMI	GCAATG	1 cut(s)	1013
BseNI	ACTGG	8 cut(s)	62, 77, 246, 895, 1255, 1722, 2129, 2348
BseRI	GAGGAG	7 cut(s)	48, 153, 156, 705, 1234, 2505, 2508
BseSI	GKGCMC	1 cut(s)	596
BseXI	GCAGC	5 cut(s)	995, 1087, 1403, 1453, 2170
BsgI	GTGCAG	1 cut(s)	1086
Bsh1236I	CGCG	1 cut(s)	238
BshFI	GGCC	5 cut(s)	226, 824, 1706, 2234, 2574
BshNI	GGYRCC	2 cut(s)	490, 874
BshVI	ATCGAT	1 cut(s)	621
BsiHKCI	CYCGRG	2 cut(s)	148, 1298
BsiSI	CCGG	5 cut(s)	149, 202, 609, 1732, 1824
BslFI	GGGAC	3 cut(s)	165, 2389, 2582
BslI	CCNNNNNNNGG	3 cut(s)	602, 1028, 1270
BsmAI	GTCTC	8 cut(s)	359, 894, 991, 1263, 1538, 1543, 2060, 2081
BsmBI	CGTCTC	1 cut(s)	1538
BsmFI	GGGAC	3 cut(s)	165, 2389, 2582
BsmI	GAATGC	2 cut(s)	1700, 2076
BsnI	GGCC	5 cut(s)	226, 824, 1706, 2234, 2574
Bso31I	GGTCTC	1 cut(s)	359
BsoBI	CYCGRG	2 cut(s)	148, 1298
Bsp1286I	GDGCHC	1 cut(s)	596
Bsp1407I	TGTACA	2 cut(s)	755, 1597
Bsp1720I	GCTNAGC	1 cut(s)	2286
Bsp19I	CCATGG	1 cut(s)	411
BspACI	CCGC	3 cut(s)	236, 2156, 2575
BspANI	GGCC	5 cut(s)	226, 824, 1706, 2234, 2574
BspDI	ATCGAT	1 cut(s)	621
BspFNI	CGCG	1 cut(s)	238
BspHI	TCATGA	1 cut(s)	2533
BspLI	GGNNCC	6 cut(s)	146, 492, 607, 775, 876, 1009
BspMAI	CTGCAG	1 cut(s)	333
BspMI	ACCTGC	1 cut(s)	949
BspPI	GGATC	5 cut(s)	401, 600, 613, 1297, 2040
BspQI	GCTCTTC	3 cut(s)	913, 1298, 2557
BspT107I	GGYRCC	2 cut(s)	490, 874
BspTNI	GGTCTC	1 cut(s)	359
BsrDI	GCAATG	1 cut(s)	1013
BsrFI	RCCGGY	2 cut(s)	1731, 1823
BsrGI	TGTACA	2 cut(s)	755, 1597
BsrI	ACTGG	8 cut(s)	62, 77, 246, 895, 1255, 1722, 2129, 2348
BssAI	RCCGGY	2 cut(s)	1731, 1823
BssECI	CCNNGG	7 cut(s)	148, 378, 411, 649, 835, 1022, 1501
BssNI	GRCGYC	1 cut(s)	1922
BssT1I	CCWWGG	4 cut(s)	411, 649, 835, 1501
Bst2UI	CCWGG	5 cut(s)	280, 310, 686, 720, 2298
Bst4CI	ACNGT	9 cut(s)	301, 362, 374, 425, 1051, 1270, 1424, 1537, 2459
Bst6I	CTCTTC	5 cut(s)	864, 913, 1298, 2334, 2557
BstACI	GRCGYC	1 cut(s)	1922
BstAPI	GCANNNNNTGC	1 cut(s)	1416
BstAUI	TGTACA	2 cut(s)	755, 1597
BstC8I	GCNNGC	7 cut(s)	810, 845, 946, 1076, 1412, 1498, 2548
BstDSI	CCRYGG	1 cut(s)	411
BstF5I	GGATG	7 cut(s)	694, 1219, 1511, 1658, 1721, 1733, 1748
BstFNI	CGCG	1 cut(s)	238
BstH2I	RGCGCY	1 cut(s)	1417
BstHHI	GCGC	1 cut(s)	1416
BstMAI	GTCTC	8 cut(s)	359, 894, 991, 1263, 1538, 1543, 2060, 2081
BstMWI	GCNNNNNNNGC	5 cut(s)	131, 950, 1416, 1506, 2282
BstNI	CCWGG	5 cut(s)	280, 310, 686, 720, 2298
BstNSI	RCATGY	4 cut(s)	134, 812, 847, 2199
BstSCI	CCNGG	8 cut(s)	147, 148, 201, 278, 308, 684, 718, 2296
BstSFI	CTRYAG	2 cut(s)	329, 912
BstSLI	GKGCMC	1 cut(s)	596
BstUI	CGCG	1 cut(s)	238
BstV1I	GCAGC	5 cut(s)	995, 1087, 1403, 1453, 2170
BstV2I	GAAGAC	1 cut(s)	73
BstX2I	RGATCY	4 cut(s)	605, 1289, 2045, 2218
BstXI	CCANNNNNNTGG	1 cut(s)	418
BstYI	RGATCY	4 cut(s)	605, 1289, 2045, 2218
Bsu15I	ATCGAT	1 cut(s)	621
BsuRI	GGCC	5 cut(s)	226, 824, 1706, 2234, 2574
BsuTUI	ATCGAT	1 cut(s)	621
BtgI	CCRYGG	1 cut(s)	411
BtrI	CACGTC	1 cut(s)	180
BtsCI	GGATG	7 cut(s)	694, 1219, 1511, 1658, 1721, 1733, 1748
BtsI	GCAGTG	2 cut(s)	141, 1933
BtsIMutI	CAGTG	5 cut(s)	141, 253, 1729, 1933, 2122
BveI	ACCTGC	1 cut(s)	949
Cac8I	GCNNGC	7 cut(s)	810, 845, 946, 1076, 1412, 1498, 2548
CciI	TCATGA	1 cut(s)	2533
CfoI	GCGC	1 cut(s)	1416
Cfr10I	RCCGGY	2 cut(s)	1731, 1823
Cfr13I	GGNCC	1 cut(s)	514
Cfr9I	CCCGGG	1 cut(s)	148
ClaI	ATCGAT	1 cut(s)	621
CseI	GACGC	3 cut(s)	1271, 1930, 2189
Csp6I	GTAC	5 cut(s)	297, 426, 756, 974, 1598
CviQI	GTAC	5 cut(s)	297, 426, 756, 974, 1598
EaeI	YGGCCR	1 cut(s)	2572
Eam1104I	CTCTTC	5 cut(s)	864, 913, 1298, 2334, 2557
EarI	CTCTTC	5 cut(s)	864, 913, 1298, 2334, 2557
Eco130I	CCWWGG	4 cut(s)	411, 649, 835, 1501
Eco147I	AGGCCT	2 cut(s)	226, 2234
Eco31I	GGTCTC	1 cut(s)	359
Eco47I	GGWCC	1 cut(s)	514
Eco47III	AGCGCT	1 cut(s)	1415
Eco57I	CTGAAG	4 cut(s)	67, 845, 2471, 2538
Eco88I	CYCGRG	2 cut(s)	148, 1298
EcoO109I	RGGNCCY	1 cut(s)	514
EcoRI	GAATTC	3 cut(s)	430, 1225, 2301
EcoRII	CCWGG	5 cut(s)	278, 308, 684, 718, 2296
EcoT14I	CCWWGG	4 cut(s)	411, 649, 835, 1501
EcoT22I	ATGCAT	2 cut(s)	478, 849
ErhI	CCWWGG	4 cut(s)	411, 649, 835, 1501
Esp3I	CGTCTC	1 cut(s)	1538
FalI	AAGNNNNNCTT	4 cut(s)	501, 533, 1027, 1059
FaqI	GGGAC	3 cut(s)	165, 2389, 2582
FauI	CCCGC	1 cut(s)	243
FbaI	TGATCA	1 cut(s)	697
FblI	GTMKAC	2 cut(s)	679, 1883
Fnu4HI	GCNGC	6 cut(s)	984, 1101, 1417, 1467, 2184, 2575
FokI	GGATG	7 cut(s)	701, 1226, 1518, 1665, 1728, 1740, 1755
Fsp4HI	GCNGC	6 cut(s)	984, 1101, 1417, 1467, 2184, 2575
FspBI	CTAG	4 cut(s)	1356, 1604, 2319, 2661
GlaI	GCGC	1 cut(s)	1415
GluI	GCNGC	6 cut(s)	984, 1101, 1417, 1467, 2184, 2575
GsuI	CTGGAG	5 cut(s)	45, 1361, 1491, 1705, 2105
HaeII	RGCGCY	1 cut(s)	1417
HaeIII	GGCC	5 cut(s)	226, 824, 1706, 2234, 2574
HapII	CCGG	5 cut(s)	149, 202, 609, 1732, 1824
HgaI	GACGC	3 cut(s)	1271, 1930, 2189
HhaI	GCGC	1 cut(s)	1416
Hin1I	GRCGYC	1 cut(s)	1922
Hin6I	GCGC	1 cut(s)	1414
HinP1I	GCGC	1 cut(s)	1414
HincII	GTYRAC	1 cut(s)	925
HindII	GTYRAC	1 cut(s)	925
HpaI	GTTAAC	1 cut(s)	925
HpaII	CCGG	5 cut(s)	149, 202, 609, 1732, 1824
Hpy166II	GTNNAC	6 cut(s)	177, 680, 925, 1186, 1884, 2345
Hpy8I	GTNNAC	6 cut(s)	177, 680, 925, 1186, 1884, 2345
Hpy99I	CGWCG	1 cut(s)	2183
HpyCH4III	ACNGT	9 cut(s)	301, 362, 374, 425, 1051, 1270, 1424, 1537, 2459
HpyCH4IV	ACGT	3 cut(s)	179, 724, 853
HpyF10VI	GCNNNNNNNGC	5 cut(s)	131, 950, 1416, 1506, 2282
HpySE526I	ACGT	3 cut(s)	179, 724, 853
Hsp92I	GRCGYC	1 cut(s)	1922
HspAI	GCGC	1 cut(s)	1414
Ksp22I	TGATCA	1 cut(s)	697
KspAI	GTTAAC	1 cut(s)	925
LguI	GCTCTTC	3 cut(s)	913, 1298, 2557
LmnI	GCTCC	6 cut(s)	817, 1007, 1247, 1591, 1724, 2559
Lsp1109I	GCAGC	5 cut(s)	995, 1087, 1403, 1453, 2170
LweI	GCATC	7 cut(s)	485, 526, 1087, 1496, 1643, 1706, 1718
MaeI	CTAG	4 cut(s)	1356, 1604, 2319, 2661
MaeII	ACGT	3 cut(s)	179, 724, 853
MaeIII	GTNAC	7 cut(s)	72, 86, 180, 1400, 1814, 1985, 2350
MfeI	CAATTG	1 cut(s)	1657
MflI	RGATCY	4 cut(s)	605, 1289, 2045, 2218
MhlI	GDGCHC	1 cut(s)	596
MlyI	GAGTC	2 cut(s)	80, 1482
MmeI	TCCRAC	2 cut(s)	583, 1693
Mph1103I	ATGCAT	2 cut(s)	478, 849
MroXI	GAANNNNTTC	1 cut(s)	1614
MseI	TTAA	5 cut(s)	924, 1046, 1512, 1800, 2472
MslI	CAYNNNNRTG	6 cut(s)	416, 567, 687, 1154, 2357, 2532
MspA1I	CMGCKG	1 cut(s)	2290
MspI	CCGG	5 cut(s)	149, 202, 609, 1732, 1824
MspR9I	CCNGG	8 cut(s)	149, 150, 203, 280, 310, 686, 720, 2298
MunI	CAATTG	1 cut(s)	1657
Mva1269I	GAATGC	2 cut(s)	1700, 2076
MvaI	CCWGG	5 cut(s)	280, 310, 686, 720, 2298
MvnI	CGCG	1 cut(s)	238
MwoI	GCNNNNNNNGC	5 cut(s)	131, 950, 1416, 1506, 2282
NciI	CCSGG	3 cut(s)	149, 150, 203
NcoI	CCATGG	1 cut(s)	411
NlaIV	GGNNCC	6 cut(s)	146, 492, 607, 775, 876, 1009
NmuCI	GTSAC	5 cut(s)	72, 180, 1400, 1814, 2350
NsiI	ATGCAT	2 cut(s)	478, 849
NspI	RCATGY	4 cut(s)	134, 812, 847, 2199
PaeI	GCATGC	2 cut(s)	812, 847
PagI	TCATGA	1 cut(s)	2533
PaqCI	CACCTGC	1 cut(s)	949
PceI	AGGCCT	2 cut(s)	226, 2234
PciSI	GCTCTTC	3 cut(s)	913, 1298, 2557
PctI	GAATGC	2 cut(s)	1700, 2076
PdmI	GAANNNNTTC	1 cut(s)	1614
PfeI	GAWTC	9 cut(s)	5, 53, 305, 464, 482, 1316, 1324, 1898, 2003
PflMI	CCANNNNNTGG	2 cut(s)	602, 1270
PfoI	TCCNGGA	4 cut(s)	201, 308, 718, 2296
PkrI	GCNGC	6 cut(s)	985, 1102, 1418, 1468, 2185, 2576
PleI	GAGTC	2 cut(s)	79, 1481
PpsI	GAGTC	2 cut(s)	79, 1481
PpuMI	RGGWCCY	1 cut(s)	514
Psp5II	RGGWCCY	1 cut(s)	514
Psp6I	CCWGG	5 cut(s)	278, 308, 684, 718, 2296
PspGI	CCWGG	5 cut(s)	278, 308, 684, 718, 2296
PspN4I	GGNNCC	6 cut(s)	146, 492, 607, 775, 876, 1009
PspPI	GGNCC	1 cut(s)	514
PspPPI	RGGWCCY	1 cut(s)	514
PstI	CTGCAG	1 cut(s)	333
PsuI	RGATCY	4 cut(s)	605, 1289, 2045, 2218
PvuII	CAGCTG	1 cut(s)	2290
RsaI	GTAC	5 cut(s)	298, 427, 757, 975, 1599
RsaNI	GTAC	5 cut(s)	297, 426, 756, 974, 1598
RseI	CAYNNNNRTG	6 cut(s)	416, 567, 687, 1154, 2357, 2532
SapI	GCTCTTC	3 cut(s)	913, 1298, 2557
SaqAI	TTAA	5 cut(s)	924, 1046, 1512, 1800, 2472
SatI	GCNGC	6 cut(s)	984, 1101, 1417, 1467, 2184, 2575
Sau96I	GGNCC	1 cut(s)	514
SchI	GAGTC	2 cut(s)	80, 1482
ScrFI	CCNGG	8 cut(s)	149, 150, 203, 280, 310, 686, 720, 2298
SduI	GDGCHC	1 cut(s)	596
SfaNI	GCATC	7 cut(s)	485, 526, 1087, 1496, 1643, 1706, 1718
SfcI	CTRYAG	2 cut(s)	329, 912
SinI	GGWCC	1 cut(s)	514
SmaI	CCCGGG	1 cut(s)	150
SmiMI	CAYNNNNRTG	6 cut(s)	416, 567, 687, 1154, 2357, 2532
SmlI	CTYRAG	2 cut(s)	1541, 2563
SmoI	CTYRAG	2 cut(s)	1541, 2563
SpeI	ACTAGT	1 cut(s)	2660
SphI	GCATGC	2 cut(s)	812, 847
SseBI	AGGCCT	2 cut(s)	226, 2234
SsiI	CCGC	3 cut(s)	236, 2156, 2575
SspI	AATATT	1 cut(s)	1688
SspMI	CTAG	4 cut(s)	1356, 1604, 2319, 2661
StuI	AGGCCT	2 cut(s)	226, 2234
StyD4I	CCNGG	8 cut(s)	147, 148, 201, 278, 308, 684, 718, 2296
StyI	CCWWGG	4 cut(s)	411, 649, 835, 1501
TaaI	ACNGT	9 cut(s)	301, 362, 374, 425, 1051, 1270, 1424, 1537, 2459
TaiI	ACGT	3 cut(s)	182, 727, 856
TaqI	TCGA	3 cut(s)	390, 621, 1993
TatI	WGTACW	2 cut(s)	755, 1597
TauI	GCSGC	1 cut(s)	2577
TfiI	GAWTC	9 cut(s)	5, 53, 305, 464, 482, 1316, 1324, 1898, 2003
Tru1I	TTAA	5 cut(s)	924, 1046, 1512, 1800, 2472
Tru9I	TTAA	5 cut(s)	924, 1046, 1512, 1800, 2472
TscAI	CASTG	5 cut(s)	141, 253, 1729, 1940, 2129
TseFI	GTSAC	5 cut(s)	72, 180, 1400, 1814, 2350
TseI	GCWGC	5 cut(s)	983, 1100, 1416, 1466, 2183
Tsp45I	GTSAC	5 cut(s)	72, 180, 1400, 1814, 2350
TspDTI	ATGAA	9 cut(s)	288, 408, 647, 1181, 1230, 1542, 2046, 2321, 2572
TspGWI	ACGGA	1 cut(s)	537
TspMI	CCCGGG	1 cut(s)	148
TspRI	CASTG	5 cut(s)	141, 253, 1729, 1940, 2129
Van91I	CCANNNNNTGG	2 cut(s)	602, 1270
VpaK11BI	GGWCC	1 cut(s)	514
XapI	RAATTY	7 cut(s)	15, 430, 1225, 1274, 1614, 2059, 2301
XceI	RCATGY	4 cut(s)	134, 812, 847, 2199
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1000
XmaI	CCCGGG	1 cut(s)	148
XmiI	GTMKAC	2 cut(s)	679, 1883
XmnI	GAANNNNTTC	1 cut(s)	1614
XspI	CTAG	4 cut(s)	1356, 1604, 2319, 2661
Zsp2I	ATGCAT	2 cut(s)	478, 849

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.