MD08G1149000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0014086
Physical Location & Seq
Reverse (-)
14986003 .. 14986881
879 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1149000.v1.1.491

Sequence Viewer

Length: 879 bp
ATGTCCTCCTCCGTTGTAATTAGCCAAGAGGCGCTCCACATTATCAACACCACCAATCGAGAAATTTTTTCTCAATTGGTACTCGCTCTCCATCGTGACTTAGCAGAATCCTTGATGATTATGGCGTTGTGGCTCTTTCTCCAGGAGAAGGGCTACAACAAATTCGTGTACAAGATGGTGAGGCTTTCAAACGCTGTACTCAATGGTTTAGCTAATGAAGCTGTCCAATGCTTGAAGTGCTTAGAGTCTACCAATTGCCCTAGAACCATTTCAAGAACTGCACCCAATTGTGGTAGCCTGCTCCTCACTAAAAGGCTCCTGGGGAAGGAAATTTCACTCCAAATCGTCAGCATAAACCGATACACATTAATCAGTGGGGTAAAAAATTTCGTTACCAATGTCTGTGCTAGAATTTTTACTGACATTTTGCAGCGGATTTTGTTTCCGCGCTCCTACGTACATTTTAGTCCGGAGGATACTTTTGTAATCCCATTATTTCCTAACCGGCTGTTTGGTAGCCTGAAAATAGTTAACCCCAACAGCTCCATCAACCACGGTGTTCCTACCGGAGGGATATGGGGTTGGAGCCCTTTCCTTGAACTGTCAGTGGATGATAGGACTATGTTCCTAACATTTTCGAGAGGCTTCCCTGTGTCACAGGATGAGGTCAAGGAGTTATTCATGGAACTTTTGGGATCTGAAACCTGTGTGGAACGTGTGCAAATGGCAAATGTTCCTCCTAATAAACAACCTTTGTATGCTAAACTGGTGTTGAGTACCGTCGTTTATGTTGATCGTGTTCTGAGAGGGACTCGAGTTTCGAAGTTCAGGATCAACAGAAAACACATTTGGGCGAGGAAGTATGAGCGAAGGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

293

Amino Acids

33.34

Weight (kDa)

9.69

Isoelectric Point (pI)

42.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014086)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G64870 AT3G45200 AT5G11220 AT5G24355
fragaria_vesca FvH4_2g29280
malus_domestica MD08G1149000.v1.1 MD15G1124100.v1.1
prunus_persica Prupe.1G479500_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0298091
rosa_laevigata RLG00000011535
rosa_multiflora Rmu_sc0002800.1_g000005
rosa_rugosa Rorug06G0278800
rosa_samantha Rh6AG388900 Rh6BG397200 Rh6CG403100 Rh6DG389700
rosa_wichuraiana Rw6G033960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 248
AccII CGCG 1 cut(s) 448
AccIII TCCGGA 1 cut(s) 469
AciI CCGC 2 cut(s) 433, 446
AclWI GGATC 2 cut(s) 703, 839
AcsI RAATTY 5 cut(s) 63, 161, 330, 385, 411
AfaI GTAC 5 cut(s) 81, 170, 198, 459, 778
AfiI CCNNNNNNNGG 4 cut(s) 148, 290, 325, 569
AflIII ACRYGT 1 cut(s) 715
AgsI TTSAA 4 cut(s) 189, 235, 273, 599
AjnI CCWGG 2 cut(s) 141, 318
AjuI GAANNNNNNNTTGG 2 cut(s) 832, 864
AluBI AGCT 3 cut(s) 212, 221, 543
AluI AGCT 3 cut(s) 212, 221, 543
AlwI GGATC 2 cut(s) 703, 839
Ama87I CYCGRG 1 cut(s) 813
Aor13HI TCCGGA 1 cut(s) 469
ApeKI GCWGC 1 cut(s) 430
ApoI RAATTY 5 cut(s) 63, 161, 330, 385, 411
ArsI GACNNNNNNTTYG 2 cut(s) 802, 834
AseI ATTAAT 1 cut(s) 368
Asp700I GAANNNNTTC 1 cut(s) 268
AspLEI GCGC 2 cut(s) 34, 450
AsuHPI GGTGA 1 cut(s) 190
AsuII TTCGAA 1 cut(s) 821
AvaI CYCGRG 1 cut(s) 813
BaeI ACNNNNGTAYC 2 cut(s) 468, 501
BanII GRGCYC 1 cut(s) 590
BbvI GCAGC 1 cut(s) 442
BccI CCATC 3 cut(s) 99, 169, 554
BciT130I CCWGG 2 cut(s) 143, 320
BciVI GTATCC 1 cut(s) 469
BfaI CTAG 2 cut(s) 261, 408
BfoI RGCGCY 1 cut(s) 35
BfuI GTATCC 1 cut(s) 469
BisI GCNGC 1 cut(s) 431
BlsI GCNGC 1 cut(s) 432
Bme1390I CCNGG 2 cut(s) 143, 320
BmeT110I CYCGRG 1 cut(s) 813
BmiI GGNNCC 2 cut(s) 317, 587
BmrFI CCNGG 2 cut(s) 143, 320
BplI GAGNNNNNCTC 2 cut(s) 796, 828
BpmI CTGGAG 1 cut(s) 125
Bpu14I TTCGAA 1 cut(s) 821
BsaAI YACGTR 1 cut(s) 457
BsaJI CCNNGG 2 cut(s) 319, 553
BsaWI WCCGGW 2 cut(s) 469, 566
BsaXI ACNNNNNCTCC 2 cut(s) 72, 102
Bsc4I CCNNNNNNNGG 4 cut(s) 148, 290, 325, 569
Bse118I RCCGGY 1 cut(s) 504
Bse1I ACTGG 1 cut(s) 771
BseAI TCCGGA 1 cut(s) 469
BseBI CCWGG 2 cut(s) 143, 320
BseDI CCNNGG 2 cut(s) 319, 553
BseGI GGATG 2 cut(s) 616, 667
BseLI CCNNNNNNNGG 4 cut(s) 148, 290, 325, 569
BseMII CTCAG 1 cut(s) 794
BseNI ACTGG 1 cut(s) 771
BseRI GAGGAG 1 cut(s) 293
BseXI GCAGC 1 cut(s) 442
BsgI GTGCAG 1 cut(s) 264
Bsh1236I CGCG 1 cut(s) 448
BsiHKCI CYCGRG 1 cut(s) 813
BsiSI CCGG 3 cut(s) 470, 505, 567
BslFI GGGAC 1 cut(s) 823
BslI CCNNNNNNNGG 4 cut(s) 148, 290, 325, 569
BsmFI GGGAC 1 cut(s) 823
BsoBI CYCGRG 1 cut(s) 813
Bsp119I TTCGAA 1 cut(s) 821
Bsp1286I GDGCHC 1 cut(s) 590
Bsp13I TCCGGA 1 cut(s) 469
Bsp1407I TGTACA 1 cut(s) 168
Bsp143I GATC 3 cut(s) 695, 793, 831
BspACI CCGC 2 cut(s) 433, 446
BspCNI CTCAG 1 cut(s) 795
BspEI TCCGGA 1 cut(s) 469
BspFNI CGCG 1 cut(s) 448
BspLI GGNNCC 2 cut(s) 317, 587
BspPI GGATC 2 cut(s) 703, 839
BspT104I TTCGAA 1 cut(s) 821
BsrFI RCCGGY 1 cut(s) 504
BsrGI TGTACA 1 cut(s) 168
BsrI ACTGG 1 cut(s) 771
BssAI RCCGGY 1 cut(s) 504
BssECI CCNNGG 2 cut(s) 319, 553
BssMI GATC 3 cut(s) 695, 793, 831
Bst2UI CCWGG 2 cut(s) 143, 320
Bst4CI ACNGT 3 cut(s) 557, 603, 781
BstAUI TGTACA 1 cut(s) 168
BstBAI YACGTR 1 cut(s) 457
BstBI TTCGAA 1 cut(s) 821
BstC8I GCNNGC 1 cut(s) 299
BstDEI CTNAG 3 cut(s) 100, 241, 803
BstDSI CCRYGG 1 cut(s) 553
BstENI CCTNNNNNAGG 2 cut(s) 323, 567
BstF5I GGATG 2 cut(s) 616, 667
BstFNI CGCG 1 cut(s) 448
BstH2I RGCGCY 1 cut(s) 35
BstHHI GCGC 2 cut(s) 34, 450
BstKTI GATC 3 cut(s) 698, 796, 834
BstMBI GATC 3 cut(s) 695, 793, 831
BstMWI GCNNNNNNNGC 2 cut(s) 218, 237
BstNI CCWGG 2 cut(s) 143, 320
BstSCI CCNGG 2 cut(s) 141, 318
BstSNI TACGTA 1 cut(s) 457
BstUI CGCG 1 cut(s) 448
BstV1I GCAGC 1 cut(s) 442
BstX2I RGATCY 1 cut(s) 695
BstYI RGATCY 1 cut(s) 695
BsuI GTATCC 1 cut(s) 469
BtgI CCRYGG 1 cut(s) 553
BtsCI GGATG 2 cut(s) 616, 667
BtsIMutI CAGTG 2 cut(s) 379, 612
Cac8I GCNNGC 1 cut(s) 299
CfoI GCGC 2 cut(s) 34, 450
Cfr10I RCCGGY 1 cut(s) 504
Csp6I GTAC 5 cut(s) 80, 169, 197, 458, 777
CviAII CATG 1 cut(s) 682
CviQI GTAC 5 cut(s) 80, 169, 197, 458, 777
DdeI CTNAG 3 cut(s) 100, 241, 803
DpnI GATC 3 cut(s) 697, 795, 833
DpnII GATC 3 cut(s) 695, 793, 831
Eco105I TACGTA 1 cut(s) 457
Eco24I GRGCYC 1 cut(s) 590
Eco88I CYCGRG 1 cut(s) 813
EcoNI CCTNNNNNAGG 2 cut(s) 323, 567
EcoRII CCWGG 2 cut(s) 141, 318
EcoT38I GRGCYC 1 cut(s) 590
FaeI CATG 1 cut(s) 685
FaiI YATR 8 cut(s) 122, 353, 577, 623, 683, 759, 789, 864
FaqI GGGAC 1 cut(s) 823
FatI CATG 1 cut(s) 681
FblI GTMKAC 1 cut(s) 248
Fnu4HI GCNGC 1 cut(s) 431
FokI GGATG 2 cut(s) 623, 674
FriOI GRGCYC 1 cut(s) 590
Fsp4HI GCNGC 1 cut(s) 431
FspBI CTAG 2 cut(s) 261, 408
GlaI GCGC 2 cut(s) 33, 449
GluI GCNGC 1 cut(s) 431
GsuI CTGGAG 1 cut(s) 125
HaeII RGCGCY 1 cut(s) 35
HapII CCGG 3 cut(s) 470, 505, 567
HhaI GCGC 2 cut(s) 34, 450
Hin1II CATG 1 cut(s) 685
Hin6I GCGC 2 cut(s) 32, 448
HinP1I GCGC 2 cut(s) 32, 448
HincII GTYRAC 1 cut(s) 532
HindII GTYRAC 1 cut(s) 532
HinfI GANTC 3 cut(s) 107, 245, 811
HpaI GTTAAC 1 cut(s) 532
HpaII CCGG 3 cut(s) 470, 505, 567
HphI GGTGA 1 cut(s) 190
Hpy166II GTNNAC 3 cut(s) 169, 249, 532
Hpy188I TCNGA 2 cut(s) 700, 804
Hpy188III TCNNGA 6 cut(s) 59, 95, 273, 470, 639, 829
Hpy8I GTNNAC 3 cut(s) 169, 249, 532
Hpy99I CGWCG 1 cut(s) 785
HpyAV CCTTC 3 cut(s) 142, 319, 864
HpyCH4III ACNGT 3 cut(s) 557, 603, 781
HpyCH4IV ACGT 2 cut(s) 456, 715
HpyCH4V TGCA 3 cut(s) 281, 430, 721
HpyF10VI GCNNNNNNNGC 2 cut(s) 218, 237
HpyF3I CTNAG 3 cut(s) 100, 241, 803
HpySE526I ACGT 2 cut(s) 456, 715
Hsp92II CATG 1 cut(s) 685
HspAI GCGC 2 cut(s) 32, 448
Kpn2I TCCGGA 1 cut(s) 469
KspAI GTTAAC 1 cut(s) 532
Kzo9I GATC 3 cut(s) 695, 793, 831
LmnI GCTCC 6 cut(s) 39, 306, 321, 455, 548, 585
Lsp1109I GCAGC 1 cut(s) 442
MaeI CTAG 2 cut(s) 261, 408
MaeII ACGT 2 cut(s) 456, 715
MaeIII GTNAC 3 cut(s) 95, 391, 654
MalI GATC 3 cut(s) 697, 795, 833
MboI GATC 3 cut(s) 695, 793, 831
MfeI CAATTG 3 cut(s) 74, 253, 286
MflI RGATCY 1 cut(s) 695
MhlI GDGCHC 1 cut(s) 590
MluCI AATT 9 cut(s) 18, 63, 74, 161, 253, 286, 330, 385, 411
MlyI GAGTC 2 cut(s) 254, 805
MmeI TCCRAC 1 cut(s) 563
MroI TCCGGA 1 cut(s) 469
MroXI GAANNNNTTC 1 cut(s) 268
MseI TTAA 2 cut(s) 368, 531
MspA1I CMGCKG 1 cut(s) 433
MspI CCGG 3 cut(s) 470, 505, 567
MspR9I CCNGG 2 cut(s) 143, 320
MunI CAATTG 3 cut(s) 74, 253, 286
MvaI CCWGG 2 cut(s) 143, 320
MvnI CGCG 1 cut(s) 448
MwoI GCNNNNNNNGC 2 cut(s) 218, 237
NdeII GATC 3 cut(s) 695, 793, 831
NlaIII CATG 1 cut(s) 685
NlaIV GGNNCC 2 cut(s) 317, 587
NmuCI GTSAC 2 cut(s) 95, 654
NspV TTCGAA 1 cut(s) 821
PaeR7I CTCGAG 1 cut(s) 813
PdmI GAANNNNTTC 1 cut(s) 268
PfeI GAWTC 1 cut(s) 107
PfoI TCCNGGA 1 cut(s) 141
PkrI GCNGC 1 cut(s) 432
PleI GAGTC 2 cut(s) 253, 805
PpsI GAGTC 2 cut(s) 253, 805
Ppu21I YACGTR 1 cut(s) 457
PshBI ATTAAT 1 cut(s) 368
Psp6I CCWGG 2 cut(s) 141, 318
PspGI CCWGG 2 cut(s) 141, 318
PspN4I GGNNCC 2 cut(s) 317, 587
PspXI VCTCGAGB 1 cut(s) 813
PsuI RGATCY 1 cut(s) 695
RsaI GTAC 5 cut(s) 81, 170, 198, 459, 778
RsaNI GTAC 5 cut(s) 80, 169, 197, 458, 777
SaqAI TTAA 2 cut(s) 368, 531
SatI GCNGC 1 cut(s) 431
Sau3AI GATC 3 cut(s) 695, 793, 831
SchI GAGTC 2 cut(s) 254, 805
ScrFI CCNGG 2 cut(s) 143, 320
SduI GDGCHC 1 cut(s) 590
SetI ASST 8 cut(s) 214, 223, 459, 545, 669, 707, 718, 754
Sfr274I CTCGAG 1 cut(s) 813
SfuI TTCGAA 1 cut(s) 821
SlaI CTCGAG 1 cut(s) 813
SmlI CTYRAG 1 cut(s) 813
SmoI CTYRAG 1 cut(s) 813
SnaBI TACGTA 1 cut(s) 457
Sse9I AATT 9 cut(s) 18, 63, 74, 161, 253, 286, 330, 385, 411
SsiI CCGC 2 cut(s) 433, 446
SspMI CTAG 2 cut(s) 261, 408
StyD4I CCNGG 2 cut(s) 141, 318
TaaI ACNGT 3 cut(s) 557, 603, 781
TaiI ACGT 2 cut(s) 459, 718
TaqI TCGA 4 cut(s) 58, 638, 814, 821
TasI AATT 9 cut(s) 18, 63, 74, 161, 253, 286, 330, 385, 411
TatI WGTACW 2 cut(s) 168, 196
TfiI GAWTC 1 cut(s) 107
Tru1I TTAA 2 cut(s) 368, 531
Tru9I TTAA 2 cut(s) 368, 531
TscAI CASTG 2 cut(s) 379, 612
TseFI GTSAC 2 cut(s) 95, 654
TseI GCWGC 1 cut(s) 430
Tsp45I GTSAC 2 cut(s) 95, 654
TspDTI ATGAA 2 cut(s) 231, 670
TspRI CASTG 2 cut(s) 379, 612
VspI ATTAAT 1 cut(s) 368
XagI CCTNNNNNAGG 2 cut(s) 323, 567
XapI RAATTY 5 cut(s) 63, 161, 330, 385, 411
XhoI CTCGAG 1 cut(s) 813
XmiI GTMKAC 1 cut(s) 248
XmnI GAANNNNTTC 1 cut(s) 268
XspI CTAG 2 cut(s) 261, 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.