MD08G1159600.v1.1

Histone-lysine N-methyltransferase ASHH2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0007450
Physical Location & Seq
Forward (+)
18359822 .. 18371676
11855 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1159600.v1.1.491

Sequence Viewer

Length: 6348 bp
ATGGCTTGTGGAAATTCGATTTTAGTTGAGGGTCTTTTGTGTGAGCATGTCACCGAGCAGCAGATGGGCTCAGAGATCTTGGTGCAATCTGTTCCTGAGCAGCCATCCTGCTTAGAGCCTTTACTTGATTCTGATGTTCAACATGCCAGTGTACCAGATGGGGCTCCGGGATTGTCTAGGAATGACAATGTTGGTTGTGTGAGCTCTGGTGAGGTTATAGAGTCGTGCAAGGTTGATAGAGATGGTTTGGTGGGTGAGTGTGAGAATGCGGATGATTTGGAATTGCGAAGTACGTTGAGCGGAGAATGGAGGCTCAATAGGTGTTGCTCAACTGAGAGTCAGAGTAATGTTGATGGTTCCAATAGTGAAAGTGACCGGACGTGCTTGAAGGAAGGTGGGCCTCAACTTGAGGATGGATCTGCTGCTTTAGTTGGGGACTGTGAAATCCCTTTGGAAGTCATATGTCAGACTGGTTCATCAGGAGATGGTGTCCAACAGGATGAGCAGAGAGATGATAAGAGTTTTAGTTCTCTCTATTCTAAAGAGGTTATGGAAGTTACGGAAGAGAAAGGTGACATTTATCTTGAGATAAAAAATGATATCTGTGAACCAGTATCTCCTTTGCAGGGTGTTGAAATTCCCTCAGAACCAGCAGAAATGGAGGCTTGCAGTAGTCCCCATAAAGAAGAGAAGGGTTTCAACATCTTAGCTGGTCCCTCTTTGGAAGCTGCGTATAAGGAGAGTGTTGTTTCCAGTGGGATAGAAGATGATTTGTGCAACAGGACATCACCTGCACGAGATGTTGAAATGCATTCAGAAGTATCATTTGCATGTGATTTAGTGCCTAATTGTGACTGTAAGAATGAGCAGATGGATGATAGTGGTGTTAGTGGTCTGAGCAGCCTCTCTACTGAAAGAACTACAGAGGTTGTTGAGATGAAAAGTAATGTTGATACATGCACTCAAATATTTCCTTCGCAAGGTTTTGAGAGGGCCTTGGAAGATTTACATATGCCTGATTTACTGAGTAGTTGTGCCCAACAGAATGATCAGATTAGTGATAAAATTATTGAAGGTTTCTTGGTAGAAAGGTCTATAGAGATTCTGGAAAAAAACAGTGATGTTATGACAGATGTAGAGGCTGTAATTCTCACGCAAATATCTCCTGTAAAAGTTGATGTCCACTATTCTAAGGAAGGTCCCTCCGAAATAGCTTCCAACTGCATTATTGAGAACTCTGTTTCTATGCAGTCATGTCAAACCTTAGGGATTGCCGAGAACAGTGTCAGTGGGCTCTCTACTGATATGGTGGTTGTTATGAAAAGCAATATTAATGAAATATTGCCTCCAGAGGGTTGTCAGGGGGCCTTTGAAGGTTCACATGTGTCAGATTCACCGAGTGTTTGCACTCAAGAGAATGGTCTGAGGAACGATAAGGTTATTGATTTTGCGTCTTCTGAAAGGGTAACAGAATTTTTGGAACAGAAAAGTGATGCTACAGCCAACATGAGGTTTGAAATTGGCACCCAGATATCACTAATGGAGGAAAAGGTCTCCAATCTGAAGGAATGTTCTTGTGGACTATCCCCCTACGGTATTCATGAGAAGTCTGTTTCTCTGAAATTGTTTCAATCTTTTGATACTGTCAACAATGGCTCTTCTGAGAGTGTGGATGTGCCAGATAAGAATGATTCCCCTGGTAATGTTGATTCAAGCATTTCATTTGACCGTTCTGGAGAGAAATACCATGAAGGAAATGATAATGTCAAAGTTGGTTGTGTTTCTAAACCCAAATATCCTGACATTGTAGCATTGTCTTCTCGAAGGAGTGGCCGAAGTCGCAAGACTCAGACAAAAAGGGCCCCAAGGAAAGGCAGAAGCACATCTAAAGAGTTGCAACCACTTGGAAGCCTTGAAACTGTCTTCAAGGCTGCTGGAAGGAAGAGAAGCTGCCTTTCCAAACCAGCTCGTTCTTCTGTATGGGGATTATTAGGGAGTGTAACACAATCTTTTGAAGAGAGTAACAGGCTTGAGGTCAGTCAAGGTAAGAATCAAGGATCACAGAAACGAAGGAGTGGACAAAGAAGTGGACAACGGAATCAGAGTGGTGCTGGTGGAAATTCACAAGGGTCTAGGGGTACAAGCAGTGCTTCAACTAATCGTGTTCGCTTGAAGGTTAAAGTGGGGAAAGAAGTGTGTAAAAGTTCTCTCTACATCACAGTATCTGAGGTTGTTGATACCTCAGCATCAACAAATTCTGTTAAAAAAGGGAATGGCAATGAGGGTAATTGGAGAAATGCGACTGTTGGGGAGGATAGAACTTATCTGGATGCTCCTGTTATGGATGTTGATCTGGCTAATAAGGACTTGGAGAGTGTTGTTCTCACAGAGAATTCAGCTGAAGATGTTATTGATAATTTTCGTGGGGTTCCATCTCATTCAGTTGCTGTATCTTCAGGAGGATCAGTTGGGACTAGCTTTACAGATCCTGGAACTTCACCTGATTCAGAAATTATCAATATGGTTCCAGATGCAGATGTTGAGGCAAGACTGCAAGAAGATTCTCATGGTAATGTTTTAACTTCTGACAAGGTTTTTGCTGCTTCTGGAGACTTTAAGAGAGGAAATAAGAAGCATAAAGTCCCTCATGTGGAAAAATGTGTTTGGGAAGGTGATAAACTGTATTCAGCAAGCATGAATAAAGAAATGCAATCAGAACCAGATGCGTGCAGGCAAAGTCAGGATTTTTTCCCTAGTGAGATACTCACTTCATACACCTGTGCAAATGCTTCAAGCAACTCATCAAGTGATAAGGAATCATCTTTGGAAGCACTGCGCTTGTCAGGAGAAACTGACCATGGAATTTCTAGAGATGTTCTTAGAGTGGAACTGGGTGCAGATAATCTTGATGTGGGCTTAGGATTACCAAAATCACAATCCTCAAAATCTAAGGGACTGAAGCCTCCCAAGGTCAGGTCCAGAGGCTGTGACTCAGCAAGCAAGAAGGGTAATGCTCATAGACTGACCGAAAACCGGAAACAGTCTGCTAACAAGAAGAAAGTCATGATAAAGGCTGATGGGGATCAGGTTTCATGCAAAGTGGAAAGTCTCCCAGAATCAGGTGTTCCTAACTTGGACATGGTGCCTGTTGACTTAGATAAGCATTATTTACCACCACGTAATGCTTGGGTGCTCTGTGATGATTGTCATAAATGGAGGCATATACCAGCTGAACTTGCAGATGTTATAGATGAACAAAAGTGCACATGGACCTGTAAAGATAACAAGGATGAAGCCTTTGCTGATTGCTCAATCCCTCAAGAGAAATCGAATTCTGAGATTAACGCAGAGTTGGATATATCAGATGCCTCAGGTGATGAAGATGCATCTGGCACCCGATCAAAGTACAAAGAATTGGAATGTTGGCGTCCAACAGTTTCTCAACAGAATGTTGCAAGCATAAAAACTAATCAGTTCCTTCACCGTGGCCGTAAAACTCAGAATATTGACGAGATAATGGTTTGCCATTGCAAACCACCTTCAGATGGCCAGTTGGGTTGCGGAGATGATTGCCTGAATCGAATGCTTAATATTGAATGTGTTCGAGGAGCCTGTCCATGTAGAGACCTTTGTTCAAATCAGCAGTTCCAAAAACGCCGATATGCCAAACTAGAGAAGTTTCGAAGTGGAAAGAAGGGTTTCGGGCTCAGGTTGCTTGAGGATATATTTAAAGGGCATTTTCTTATTGAATATGTTGGAGAGGTGCTTGATACAAATGCTTATGAGGCACGACAAAAAGAGTATGCTGTGAAGGGTCACAGACATTTCTACTTCATGACATTGAATGGCAGTGAGGTAATAGATGCATGTGCAAAGGGAAATTTGGGGCGTTTCATTAACCATAGTTGTGATCCTAATTGTCGCACAGAAAAGTGGATGGTGAATGGAGAGATTTGTATAGGACTATTTGCATTGAGAGATATAAAGAAGGGTGAAGAGGTTACATTTGACTACAACTATGTAAGGGTTTTCGGAGCTGCTGCCAAAAAATGTCATTGTGGTTCATCTGTATGTCGGGGTTACATAGGTGGTGATCTTCTTGATAGTGAAGTCATTGTTCAAGATGATTCAGATGAAGAATATATGGAGCCCGTGATGATTCCAGAAGATGCTGTATCTGAAGATAGGTCTGACAATACGTTGCCTGCAAATAAAGTAATTGATGATTCAATAATTGCTATTGGGGAATTGGAGTATACCACACAAAGAGAGGAGTCTGTGAATCGATCTGATTCACTTGTTTCTCACATACACGCTCCATTGGAATTGAAGCACTCAAGACAGAAATTACCATCTTCTGTCCAACCAGTTGAAGCTTCCCAACAAACAGAAGATGTAGCTAGTATACCCATGCCTGTTATTGAACAGGAAATTTTTGGAGAAGGGGAGCCTACAGAAAAATCCTCAAACTCCTTTGAAAGACTAGAGACTACTACGACAAAAGTGCTTACCAAATCATTATCTGATGGGACTGACGGTGACAGGAAGTCCAAGTCTGGTACTATTGAAGCTGGACAGTTTCCTTCCAAAGTGCGTTCTAATGTGAAAACTTCCAAATCATCAAGTTTTGTGAAGAAAAGCAAAGTGAAGATTACCCCAAATGGTAACAAAATTCAAGTGACAGCTACCAAATCTCACGTGCTATCTATCAAGCCTCAACGATTAACAGAAGGCTCAGTTGAGGAGAAGCTTAACGAGCTGTTGGATGGCGAAGGAGGGATAAATAAACGGAAAGATTCCACTAAAGGCTACTTGAAGCTTCTGATCCTTACTGCAGTATCAGGTGATAGTGGCAGTGGGGAAGTAATTCAGAGCAATCGAGATCTTTCTATGATCCTTGATGCACTTTTGAAAACAAAGTCACGGATGGTTTTGATTGATGTAATAAATAAAAACGGTTTGAGAATGCTACACAACGTAATGAAAAAATACAGAGAAGACTTCAAAAAGATACCAATCCTCCGGAAGCTTCTGAAGGTCCTAGAGTATTTAGCTATGAAGCAGATACTTACATCGGATCATATTACTGGAGGTCCTCCCTGTGCTGGAATGGAGAGCTTTATGGAGTCAATGCTGTCACTGACAGAACACGAAGACAGACAGGTCCACCAAATAGCACGGAATTTTCGAGACAAGTGGATTCCCAGACCTTTCAGAAGACATGGCTATGTAGACAGGATTGATAGCAAGATGGAATTTAACAGAGGTTCAGACTGCAATAGATTTTCTGCATCACATGATAATTGGCGTGATCAAAGTGGAAGGTCTACTGAAGCTATAGATAGTGCCAAACAATCAATGGTCTCAACAACTTCTGTGAGTACAGGTGTCCAGGAGGTCTCTTCCGCACCTTGTATGGGTGGTTGCCCTACCAGTGAGACAAAAGTTCGCAAGCGTAAAAGCCGATGGGATCAACCTGCAGACCCAGATTCAAGTTTACTTCAGAACAGAGAACAGAAGAGTGAGTCTGGATTGAGTAGACAGTTGGCACCTAGTCCATCTCTGGTGATAGGTGAAATGGCATTGCATCTGGAGAGGGTGAGTGGAGAAGATGGAACTTGTTCCAGCAGTGTGCATGGTAACTGTCAGCAAAATGATGGAACAGAGATGAACCTTGACGATATTCCTCCTGGGTTTGATGCCCCTGCGATTTCATCTATGGCTTCGTCATCACTTTGTCCTTTGAAGTGTGCTGCCCCAGCGGTAGGGCATCCACAGGAGAAATTTGTTTCTCGCTTACCCGTCTCATACGGAATTCCCGTGTCTGTCATGCAGCAATATGGGACACTACATGCTGAAACTGTAGAGACTTGGACTGTTGCTCCTGGGATACCTTTTCATCCTTTTCCACCATTGCCCTCGTTTCCCCGTCAGAAGAAGGGCCCTTCACCTTCTCATACCGTCAATCATGTGGCAGTTAGTCAACCTGCAGAAGGAAAACATGACAGGTGCGTTCCTGCAACATCTCTCTCTAATGAAAGCATTCCTGGCACAACAGGTGCCAATCAGGCAGAGTTTAGCATTCCATGTGCAAACAACCAATATACATCTAAGCGGCCGAGAGGATTCTCAAATGATTTGGGAAGGAGATACTTTAAGCAGCAGAAGTACTGGAATAATAATAATACAAAATTTGGACCGCCATCTTCCGGCGACAGAAATGGGTTGGGGTGCAACGGAAACCACTCTGGTGGTGGAACAAACGGCGTCATAGGTGTAGGAAATGTAGTAAATGAGCATAGGAGTTTATATTATGCCAATGTCAATCAGCATCCACAACACCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001763 GO:0002682 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006464 GO:0006479 GO:0006629 GO:0006720 GO:0006721 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008213 GO:0008276 GO:0008757 GO:0009553 GO:0009555 GO:0009653 GO:0009791 GO:0009893 GO:0009908 GO:0009909 GO:0009910 GO:0009987 GO:0010016 GO:0010223 GO:0010346 GO:0010363 GO:0010452 GO:0010468 GO:0010604 GO:0010638 GO:0010941 GO:0016043 GO:0016108 GO:0016116 GO:0016278 GO:0016279 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018022 GO:0018024 GO:0018193 GO:0018205 GO:0019222 GO:0019538 GO:0022414 GO:0031056 GO:0031058 GO:0031060 GO:0031062 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0032259 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033043 GO:0033044 GO:0034968 GO:0035670 GO:0036211 GO:0040029 GO:0042054 GO:0042800 GO:0043067 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044255 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045088 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048443 GO:0048466 GO:0048467 GO:0048481 GO:0048518 GO:0048519 GO:0048522 GO:0048580 GO:0048581 GO:0048583 GO:0048608 GO:0048646 GO:0048653 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0050776 GO:0050789 GO:0050793 GO:0050794 GO:0051093 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051239 GO:0051241 GO:0051246 GO:0051247 GO:0051276 GO:0051568 GO:0060255 GO:0061458 GO:0065007 GO:0071704 GO:0071840 GO:0080090 GO:0080134 GO:0080135 GO:0090567 GO:0099402 GO:0140096 GO:1901564 GO:1902275 GO:1905269 GO:1905393 GO:2000026 GO:2000241 GO:2000242 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko00310 map00310
Pfam Domains
Protein Families
SET zf-CW

Protein Analysis

2116

Amino Acids

231.16

Weight (kDa)

5.44

Isoelectric Point (pI)

51.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CW PF07496 1063 - 1109 3.1e-12 CW-type Zinc Finger
AWS PF17907 1184 - 1219 2.1e-12 AWS domain
SET PF00856 1233 - 1339 7.5e-25 SET domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 799
AasI GACNNNNNNGTC 1 cut(s) 5162
Acc36I ACCTGC 3 cut(s) 799, 5485, 5995
AccB1I GGYRCC 5 cut(s) 1523, 3142, 3392, 5548, 6059
AccBSI CCGCTC 1 cut(s) 300
AccI GTMKAC 5 cut(s) 4256, 4405, 5232, 5327, 5539
AccIII TCCGGA 1 cut(s) 5022
AciI CCGC 7 cut(s) 269, 300, 3561, 5406, 5762, 6115, 6200
AcoI YGGCCR 4 cut(s) 1831, 3487, 3547, 6116
AcuI CTGAAG 9 cut(s) 1583, 2421, 2439, 2978, 3525, 4200, 5054, 5352, 5486
AcvI CACGTG 1 cut(s) 4699
AcyI GRCGYC 2 cut(s) 3427, 6267
AdeI CACNNNGTG 1 cut(s) 1400
AfaI GTAC 7 cut(s) 153, 292, 2140, 3407, 4561, 5383, 6170
AflIII ACRYGT 1 cut(s) 1381
AhdI GACNNNNNGTC 1 cut(s) 4546
AjiI CACGTC 1 cut(s) 381
AjnI CCWGG 6 cut(s) 1696, 2488, 5391, 5689, 5884, 6046
AleI CACNNNNGTG 1 cut(s) 6249
Alw21I GWGCWC 3 cut(s) 206, 3195, 3266
Alw44I GTGCAC 1 cut(s) 3262
AlwNI CAGNNNCTG 4 cut(s) 2225, 2447, 2489, 2985
Aor13HI TCCGGA 1 cut(s) 5022
AoxI GGCC 9 cut(s) 398, 993, 1365, 1831, 1860, 3487, 3547, 5941, 6116
ApaI GGGCCC 2 cut(s) 1864, 5945
ApaLI GTGCAC 1 cut(s) 3262
ArsI GACNNNNNNTTYG 2 cut(s) 2579, 2611
AseI ATTAAT 1 cut(s) 1332
Asp700I GAANNNNTTC 6 cut(s) 695, 3600, 3698, 4866, 5620, 6042
AspLEI GCGC 1 cut(s) 2838
AsuC2I CCSGG 1 cut(s) 168
AsuII TTCGAA 1 cut(s) 3682
AvaII GGWCC 8 cut(s) 713, 1199, 2976, 3270, 5038, 5093, 5164, 6197
AxyI CCTNAGG 2 cut(s) 1264, 3370
BaeGI GKGCMC 4 cut(s) 1039, 1864, 3266, 5945
BaeI ACNNNNGTAYC 2 cut(s) 4821, 4854
BalI TGGCCA 1 cut(s) 3549
BanI GGYRCC 5 cut(s) 1523, 3142, 3392, 5548, 6059
BanII GRGCYC 8 cut(s) 71, 166, 206, 1296, 1864, 3708, 4152, 5945
BauI CACGAG 1 cut(s) 795
BbrPI CACGTG 1 cut(s) 4699
BbsI GAAGAC 6 cut(s) 1446, 1809, 1915, 5004, 5160, 5224
Bbv12I GWGCWC 3 cut(s) 206, 3195, 3266
BbvCI CCTCAGC 1 cut(s) 2242
BceAI ACGGC 2 cut(s) 3474, 6280
BcgI CGANNNNNNTGC 2 cut(s) 4486, 4520
BciT130I CCWGG 6 cut(s) 1698, 2490, 5393, 5691, 5886, 6048
BciVI GTATCC 1 cut(s) 5883
BclI TGATCA 2 cut(s) 1048, 5311
BcnI CCSGG 1 cut(s) 168
BfmI CTRYAG 9 cut(s) 921, 1095, 1497, 4452, 4833, 5337, 5478, 5862, 5988
BfuAI ACCTGC 3 cut(s) 799, 5485, 5995
BfuI GTATCC 1 cut(s) 5883
BglI GCCNNNNNGGC 1 cut(s) 6068
BglII AGATCT 2 cut(s) 75, 4882
BmcAI AGTACT 1 cut(s) 6170
Bme1390I CCNGG 7 cut(s) 168, 1698, 2490, 5393, 5691, 5886, 6048
Bme18I GGWCC 8 cut(s) 713, 1199, 2976, 3270, 5038, 5093, 5164, 6197
BmeRI GACNNNNNGTC 1 cut(s) 4546
BmgBI CACGTC 1 cut(s) 381
BmrFI CCNGG 7 cut(s) 168, 1698, 2490, 5393, 5691, 5886, 6048
BmrI ACTGGG 1 cut(s) 2900
BmuI ACTGGG 1 cut(s) 2900
BpiI GAAGAC 6 cut(s) 1446, 1809, 1915, 5004, 5160, 5224
BpmI CTGGAG 5 cut(s) 1332, 1755, 2628, 5109, 5612
Bpu10I CCTNAGC 4 cut(s) 96, 2242, 2917, 3707
Bpu14I TTCGAA 1 cut(s) 3682
BpuEI CTTGAG 7 cut(s) 428, 605, 1395, 2051, 3303, 3737, 4321
BpuMI CCSGG 1 cut(s) 168
Bsa29I ATCGAT 1 cut(s) 4285
BsaAI YACGTR 2 cut(s) 3179, 4699
BsaBI GATNNNNATC 3 cut(s) 2349, 3081, 5015
BsaHI GRCGYC 2 cut(s) 3427, 6267
BsaI GGTCTC 4 cut(s) 1558, 3618, 5368, 5404
BsaJI CCNNGG 8 cut(s) 996, 1696, 1865, 2857, 2967, 3484, 5690, 5885
BsaWI WCCGGW 3 cut(s) 375, 3033, 5022
BsaXI ACNNNNNCTCC 8 cut(s) 474, 504, 2451, 2481, 5004, 5034, 5866, 5896
Bse21I CCTNAGG 2 cut(s) 1264, 3370
Bse3DI GCAATG 4 cut(s) 2284, 3526, 5582, 5912
Bse8I GATNNNNATC 3 cut(s) 2349, 3081, 5015
BseAI TCCGGA 1 cut(s) 5022
BseBI CCWGG 6 cut(s) 1698, 2490, 5393, 5691, 5886, 6048
BseCI ATCGAT 1 cut(s) 4285
BseDI CCNNGG 8 cut(s) 996, 1696, 1865, 2857, 2967, 3484, 5690, 5885
BseJI GATNNNNATC 3 cut(s) 2349, 3081, 5015
BseMI GCAATG 4 cut(s) 2284, 3526, 5582, 5912
BseRI GAGGAG 3 cut(s) 3621, 4286, 4757
BseSI GKGCMC 4 cut(s) 1039, 1864, 3266, 5945
BseX3I CGGCCG 1 cut(s) 6116
BseYI CCCAGC 1 cut(s) 5758
BsgI GTGCAG 3 cut(s) 777, 2749, 2916
Bsh1285I CGRYCG 1 cut(s) 6119
BshFI GGCC 9 cut(s) 400, 995, 1367, 1833, 1862, 3489, 3549, 5943, 6118
BshNI GGYRCC 5 cut(s) 1523, 3142, 3392, 5548, 6059
BshVI ATCGAT 1 cut(s) 4285
BsiEI CGRYCG 1 cut(s) 6119
BsiHKAI GWGCWC 3 cut(s) 206, 3195, 3266
BsiSI CCGG 5 cut(s) 167, 376, 3034, 5023, 6210
BslFI GGGAC 9 cut(s) 449, 660, 699, 1185, 2485, 2627, 2967, 4543, 5857
BsmBI CGTCTC 1 cut(s) 5809
BsmFI GGGAC 9 cut(s) 449, 660, 699, 1185, 2485, 2627, 2967, 4543, 5857
BsmI GAATGC 6 cut(s) 271, 811, 3588, 4971, 6042, 6081
BsnI GGCC 9 cut(s) 400, 995, 1367, 1833, 1862, 3489, 3549, 5943, 6118
Bso31I GGTCTC 4 cut(s) 1558, 3618, 5368, 5404
Bsp119I TTCGAA 1 cut(s) 3682
Bsp120I GGGCCC 2 cut(s) 1860, 5941
Bsp13I TCCGGA 1 cut(s) 5022
Bsp19I CCATGG 1 cut(s) 2857
BspACI CCGC 7 cut(s) 269, 300, 3561, 5406, 5762, 6115, 6200
BspANI GGCC 9 cut(s) 400, 995, 1367, 1833, 1862, 3489, 3549, 5943, 6118
BspDI ATCGAT 1 cut(s) 4285
BspEI TCCGGA 1 cut(s) 5022
BspHI TCATGA 3 cut(s) 1600, 3063, 3834
BspMAI CTGCAG 3 cut(s) 4837, 5482, 5992
BspMI ACCTGC 3 cut(s) 799, 5485, 5995
BspQI GCTCTTC 1 cut(s) 1663
BspT104I TTCGAA 1 cut(s) 3682
BspT107I GGYRCC 5 cut(s) 1523, 3142, 3392, 5548, 6059
BspTNI GGTCTC 4 cut(s) 1558, 3618, 5368, 5404
BsrBI CCGCTC 1 cut(s) 300
BsrDI GCAATG 4 cut(s) 2284, 3526, 5582, 5912
BssECI CCNNGG 8 cut(s) 996, 1696, 1865, 2857, 2967, 3484, 5690, 5885
BssNAI GTATAC 2 cut(s) 4257, 4406
BssNI GRCGYC 2 cut(s) 3427, 6267
BssSI CACGAG 1 cut(s) 795
BssT1I CCWWGG 4 cut(s) 996, 1865, 2857, 2967
Bst1107I GTATAC 2 cut(s) 4257, 4406
Bst2BI CACGAG 1 cut(s) 795
Bst2UI CCWGG 6 cut(s) 1698, 2490, 5393, 5691, 5886, 6048
Bst6I CTCTTC 8 cut(s) 558, 681, 1663, 1937, 2010, 3990, 5407, 5513
BstACI GRCGYC 2 cut(s) 3427, 6267
BstBAI YACGTR 2 cut(s) 3179, 4699
BstBI TTCGAA 1 cut(s) 3682
BstC8I GCNNGC 8 cut(s) 667, 2692, 2728, 2732, 2998, 3457, 4206, 5453
BstDSI CCRYGG 2 cut(s) 2857, 3484
BstENI CCTNNNNNAGG 2 cut(s) 978, 5991
BstHHI GCGC 1 cut(s) 2838
BstMCI CGRYCG 1 cut(s) 6119
BstMWI GCNNNNNNNGC 8 cut(s) 1839, 3236, 3712, 3785, 4756, 5759, 6048, 6068
BstNI CCWGG 6 cut(s) 1698, 2490, 5393, 5691, 5886, 6048
BstNSI RCATGY 7 cut(s) 50, 146, 834, 960, 1385, 3870, 5855
BstSCI CCNGG 7 cut(s) 166, 1696, 2488, 5391, 5689, 5884, 6046
BstSFI CTRYAG 9 cut(s) 921, 1095, 1497, 4452, 4833, 5337, 5478, 5862, 5988
BstSLI GKGCMC 4 cut(s) 1039, 1864, 3266, 5945
BstV2I GAAGAC 6 cut(s) 1446, 1809, 1915, 5004, 5160, 5224
BstX2I RGATCY 4 cut(s) 75, 416, 2485, 4882
BstXI CCANNNNNNTGG 1 cut(s) 6251
BstYI RGATCY 4 cut(s) 75, 416, 2485, 4882
BstZ17I GTATAC 2 cut(s) 4257, 4406
BstZI CGGCCG 1 cut(s) 6116
Bsu15I ATCGAT 1 cut(s) 4285
Bsu36I CCTNAGG 2 cut(s) 1264, 3370
BsuI GTATCC 1 cut(s) 5883
BsuRI GGCC 9 cut(s) 400, 995, 1367, 1833, 1862, 3489, 3549, 5943, 6118
BsuTUI ATCGAT 1 cut(s) 4285
BtgI CCRYGG 2 cut(s) 2857, 3484
BtrI CACGTC 1 cut(s) 381
BtsI GCAGTG 5 cut(s) 2152, 2831, 3856, 4861, 5635
BveI ACCTGC 3 cut(s) 799, 5485, 5995
Cac8I GCNNGC 8 cut(s) 667, 2692, 2728, 2732, 2998, 3457, 4206, 5453
CaiI CAGNNNCTG 4 cut(s) 2225, 2447, 2489, 2985
CciI TCATGA 3 cut(s) 1600, 3063, 3834
CfoI GCGC 1 cut(s) 2838
ClaI ATCGAT 1 cut(s) 4285
CseI GACGC 3 cut(s) 1440, 3416, 6256
Csp6I GTAC 7 cut(s) 152, 291, 2139, 3406, 4560, 5382, 6169
CviQI GTAC 7 cut(s) 152, 291, 2139, 3406, 4560, 5382, 6169
DraI TTTAAA 1 cut(s) 3730
DraIII CACNNNGTG 1 cut(s) 1400
DrdI GACNNNNNNGTC 1 cut(s) 5162
DriI GACNNNNNGTC 1 cut(s) 4546
DseDI GACNNNNNNGTC 1 cut(s) 5162
EaeI YGGCCR 4 cut(s) 1831, 3487, 3547, 6116
EagI CGGCCG 1 cut(s) 6116
Eam1104I CTCTTC 8 cut(s) 558, 681, 1663, 1937, 2010, 3990, 5407, 5513
Eam1105I GACNNNNNGTC 1 cut(s) 4546
EarI CTCTTC 8 cut(s) 558, 681, 1663, 1937, 2010, 3990, 5407, 5513
Ecl136II GAGCTC 1 cut(s) 204
EclXI CGGCCG 1 cut(s) 6116
Eco130I CCWWGG 4 cut(s) 996, 1865, 2857, 2967
Eco24I GRGCYC 8 cut(s) 71, 166, 206, 1296, 1864, 3708, 4152, 5945
Eco31I GGTCTC 4 cut(s) 1558, 3618, 5368, 5404
Eco32I GATATC 2 cut(s) 601, 1533
Eco47I GGWCC 8 cut(s) 713, 1199, 2976, 3270, 5038, 5093, 5164, 6197
Eco52I CGGCCG 1 cut(s) 6116
Eco53kI GAGCTC 1 cut(s) 204
Eco57I CTGAAG 9 cut(s) 1583, 2421, 2439, 2978, 3525, 4200, 5054, 5352, 5486
Eco72I CACGTG 1 cut(s) 4699
Eco81I CCTNAGG 2 cut(s) 1264, 3370
EcoICRI GAGCTC 1 cut(s) 204
EcoNI CCTNNNNNAGG 2 cut(s) 978, 5991
EcoO109I RGGNCCY 9 cut(s) 993, 1199, 1365, 1860, 1861, 5038, 5093, 5941, 5942
EcoRI GAATTC 3 cut(s) 2392, 3331, 5814
EcoRII CCWGG 6 cut(s) 1696, 2488, 5391, 5689, 5884, 6046
EcoRV GATATC 2 cut(s) 601, 1533
EcoT14I CCWWGG 4 cut(s) 996, 1865, 2857, 2967
EcoT22I ATGCAT 3 cut(s) 813, 3388, 3868
EcoT38I GRGCYC 8 cut(s) 71, 166, 206, 1296, 1864, 3708, 4152, 5945
ErhI CCWWGG 4 cut(s) 996, 1865, 2857, 2967
Esp3I CGTCTC 1 cut(s) 5809
FalI AAGNNNNNCTT 2 cut(s) 2134, 2166
FaqI GGGAC 9 cut(s) 449, 660, 699, 1185, 2485, 2627, 2967, 4543, 5857
FauNDI CATATG 2 cut(s) 461, 1011
FbaI TGATCA 2 cut(s) 1048, 5311
FblI GTMKAC 5 cut(s) 4256, 4405, 5232, 5327, 5539
FriOI GRGCYC 8 cut(s) 71, 166, 206, 1296, 1864, 3708, 4152, 5945
GlaI GCGC 1 cut(s) 2837
GsaI CCCAGC 1 cut(s) 5762
GsuI CTGGAG 5 cut(s) 1332, 1755, 2628, 5109, 5612
HaeIII GGCC 9 cut(s) 400, 995, 1367, 1833, 1862, 3489, 3549, 5943, 6118
HapII CCGG 5 cut(s) 167, 376, 3034, 5023, 6210
HgaI GACGC 3 cut(s) 1440, 3416, 6256
HhaI GCGC 1 cut(s) 2838
Hin1I GRCGYC 2 cut(s) 3427, 6267
Hin6I GCGC 1 cut(s) 2836
HinP1I GCGC 1 cut(s) 2836
HincII GTYRAC 3 cut(s) 1648, 3151, 5984
HindII GTYRAC 3 cut(s) 1648, 3151, 5984
HindIII AAGCTT 4 cut(s) 4374, 4748, 4817, 5027
HpaII CCGG 5 cut(s) 167, 376, 3034, 5023, 6210
HpyCH4IV ACGT 6 cut(s) 293, 380, 3178, 4199, 4698, 4977
HpyF10VI GCNNNNNNNGC 8 cut(s) 1839, 3236, 3712, 3785, 4756, 5759, 6048, 6068
HpySE526I ACGT 6 cut(s) 293, 380, 3178, 4199, 4698, 4977
Hsp92I GRCGYC 2 cut(s) 3427, 6267
HspAI GCGC 1 cut(s) 2836
Kpn2I TCCGGA 1 cut(s) 5022
Ksp22I TGATCA 2 cut(s) 1048, 5311
LguI GCTCTTC 1 cut(s) 1663
LmnI GCTCC 8 cut(s) 169, 2338, 3608, 4034, 4147, 4321, 4447, 5887
MaeII ACGT 6 cut(s) 293, 380, 3178, 4199, 4698, 4977
MbiI CCGCTC 1 cut(s) 300
MflI RGATCY 4 cut(s) 75, 416, 2485, 4882
MlsI TGGCCA 1 cut(s) 3549
MluNI TGGCCA 1 cut(s) 3549
MlyI GAGTC 7 cut(s) 230, 346, 1840, 2984, 4283, 5135, 5534
MmeI TCCRAC 7 cut(s) 517, 1242, 3333, 3455, 3736, 4387, 4743
Mox20I TGGCCA 1 cut(s) 3549
Mph1103I ATGCAT 3 cut(s) 813, 3388, 3868
MroI TCCGGA 1 cut(s) 5022
MroXI GAANNNNTTC 6 cut(s) 695, 3600, 3698, 4866, 5620, 6042
MscI TGGCCA 1 cut(s) 3549
MslI CAYNNNNRTG 7 cut(s) 147, 829, 2571, 3906, 4069, 5226, 6249
Msp20I TGGCCA 1 cut(s) 3549
MspA1I CMGCKG 3 cut(s) 2399, 3230, 5762
MspI CCGG 5 cut(s) 167, 376, 3034, 5023, 6210
MspR9I CCNGG 7 cut(s) 168, 1698, 2490, 5393, 5691, 5886, 6048
Mva1269I GAATGC 6 cut(s) 271, 811, 3588, 4971, 6042, 6081
MvaI CCWGG 6 cut(s) 1698, 2490, 5393, 5691, 5886, 6048
MwoI GCNNNNNNNGC 8 cut(s) 1839, 3236, 3712, 3785, 4756, 5759, 6048, 6068
NciI CCSGG 1 cut(s) 168
NcoI CCATGG 1 cut(s) 2857
NdeI CATATG 2 cut(s) 461, 1011
NmeAIII GCCGAG 2 cut(s) 1300, 6144
NsiI ATGCAT 3 cut(s) 813, 3388, 3868
NspI RCATGY 7 cut(s) 50, 146, 834, 960, 1385, 3870, 5855
NspV TTCGAA 1 cut(s) 3682
OliI CACNNNNGTG 1 cut(s) 6249
PagI TCATGA 3 cut(s) 1600, 3063, 3834
PaqCI CACCTGC 1 cut(s) 799
PciI ACATGT 1 cut(s) 1381
PciSI GCTCTTC 1 cut(s) 1663
PcsI WCGNNNNNNNCGW 2 cut(s) 5185, 5817
PctI GAATGC 6 cut(s) 271, 811, 3588, 4971, 6042, 6081
PdmI GAANNNNTTC 6 cut(s) 695, 3600, 3698, 4866, 5620, 6042
PfoI TCCNGGA 3 cut(s) 166, 2488, 5391
PleI GAGTC 7 cut(s) 229, 345, 1840, 2984, 4282, 5134, 5533
PmaCI CACGTG 1 cut(s) 4699
PmlI CACGTG 1 cut(s) 4699
PpsI GAGTC 7 cut(s) 229, 345, 1840, 2984, 4282, 5134, 5533
Ppu21I YACGTR 2 cut(s) 3179, 4699
PpuMI RGGWCCY 3 cut(s) 1199, 5038, 5093
PscI ACATGT 1 cut(s) 1381
PshBI ATTAAT 1 cut(s) 1332
Psp124BI GAGCTC 1 cut(s) 206
Psp5II RGGWCCY 3 cut(s) 1199, 5038, 5093
Psp6I CCWGG 6 cut(s) 1696, 2488, 5391, 5689, 5884, 6046
PspCI CACGTG 1 cut(s) 4699
PspFI CCCAGC 1 cut(s) 5758
PspGI CCWGG 6 cut(s) 1696, 2488, 5391, 5689, 5884, 6046
PspOMI GGGCCC 2 cut(s) 1860, 5941
PspPPI RGGWCCY 3 cut(s) 1199, 5038, 5093
PstI CTGCAG 3 cut(s) 4837, 5482, 5992
PstNI CAGNNNCTG 4 cut(s) 2225, 2447, 2489, 2985
PsuI RGATCY 4 cut(s) 75, 416, 2485, 4882
PvuII CAGCTG 2 cut(s) 2399, 3230
RsaI GTAC 7 cut(s) 153, 292, 2140, 3407, 4561, 5383, 6170
RsaNI GTAC 7 cut(s) 152, 291, 2139, 3406, 4560, 5382, 6169
RseI CAYNNNNRTG 7 cut(s) 147, 829, 2571, 3906, 4069, 5226, 6249
SacI GAGCTC 1 cut(s) 206
SapI GCTCTTC 1 cut(s) 1663
ScaI AGTACT 1 cut(s) 6170
SchI GAGTC 7 cut(s) 230, 346, 1840, 2984, 4283, 5135, 5534
ScrFI CCNGG 7 cut(s) 168, 1698, 2490, 5393, 5691, 5886, 6048
SfcI CTRYAG 9 cut(s) 921, 1095, 1497, 4452, 4833, 5337, 5478, 5862, 5988
SfuI TTCGAA 1 cut(s) 3682
SinI GGWCC 8 cut(s) 713, 1199, 2976, 3270, 5038, 5093, 5164, 6197
SmiMI CAYNNNNRTG 7 cut(s) 147, 829, 2571, 3906, 4069, 5226, 6249
SmlI CTYRAG 7 cut(s) 407, 584, 1410, 2030, 3318, 3716, 4336
SmoI CTYRAG 7 cut(s) 407, 584, 1410, 2030, 3318, 3716, 4336
SsiI CCGC 7 cut(s) 269, 300, 3561, 5406, 5762, 6115, 6200
SspI AATATT 5 cut(s) 969, 1330, 1341, 3505, 3592
SstI GAGCTC 1 cut(s) 206
StyD4I CCNGG 7 cut(s) 166, 1696, 2488, 5391, 5689, 5884, 6046
StyI CCWWGG 4 cut(s) 996, 1865, 2857, 2967
TaiI ACGT 6 cut(s) 296, 383, 3181, 4202, 4701, 4980
TaqI TCGA 9 cut(s) 17, 1822, 3329, 3580, 3604, 3682, 4285, 4879, 5188
TaqII GACCGA 1 cut(s) 3041
TatI WGTACW 3 cut(s) 3405, 5381, 6168
TauI GCSGC 1 cut(s) 6118
TspGWI ACGGA 7 cut(s) 575, 2110, 4804, 4939, 5194, 5826, 6252
VneI GTGCAC 1 cut(s) 3262
VpaK11BI GGWCC 8 cut(s) 713, 1199, 2976, 3270, 5038, 5093, 5164, 6197
VspI ATTAAT 1 cut(s) 1332
XagI CCTNNNNNAGG 2 cut(s) 978, 5991
XbaI TCTAGA 1 cut(s) 2867
XceI RCATGY 7 cut(s) 50, 146, 834, 960, 1385, 3870, 5855
XcmI CCANNNNNNNNNTGG 3 cut(s) 3183, 5356, 6251
XmiI GTMKAC 5 cut(s) 4256, 4405, 5232, 5327, 5539
XmnI GAANNNNTTC 6 cut(s) 695, 3600, 3698, 4866, 5620, 6042
ZrmI AGTACT 1 cut(s) 6170
Zsp2I ATGCAT 3 cut(s) 813, 3388, 3868
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.