Rmu_ssc0000192.1_g000006

Histone-lysine N-methyltransferase ASHH2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000192.1
OG0007450
Physical Location & Seq
Forward (+)
22770 .. 36222
13453 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000192.1_g000006.1.cds

Sequence Viewer

Length: 6321 bp
atggcatcgtatggaaacttgatgttcgtggacgagcctcggtgcgagtcgtttggtaccgagcagcagatgggctcggagatcttggtcgaaagtgtttctgagcagaacaactgcttggaggctcagcctttacctggtcgtaatgtggaccttaccagtgaggcggacgtgtctccggggttttgtaggaatgacaatgctggttgtgtgagctgcagtgaggtgacggagttgggtactggcgatagagatggtttggcgggtggtggtgaaagtacgagtgttatggagccggaagattttttgaacagtgagtatgggatcaataggtgtggcttggatgagaatcagaatgatgtggatggttgttctagtattggaagtggcgggatgcagttgaaggaaggctggtgtcgtgaggatggctttgctggttcatttgatgattgtgaagtgcccttgaaagtcataccttccacagggtcaccaggaaatggtattcaactggatgagcacagggatgatgataaaggtgctaatcgtctgtcttctgaagggatgatggaagttatggaagagcaaggtgaattgcccttggaatttgtagacattgatgtttgtagggattacaacatcttttctggtaattcattggaagctgctaatgaaaaaaatgttgctttgactgggatagaagctggtggtttgtgccacgggacatcacctgcacaggatggtggaatgccttcagaagtatcaaacacaggtgatctagtgactaactgtaactggaataaagaacatattgacgataatggtgtcagtggtgtaagcgggctctctactgaaaagtccacagaggtttctgaggcatgcaaccaaatgttgccgtcactgagttttcaaggatctttggaagatttgcacatgcctaattcactgagtaattttgcccagcagagtgatccaaggggtaataatgcagttgatggttccttggctgacagggttacagaatttatggaagagaaaagtaatgttatgacagacaaggaggctgaaattcacacccagatatcaactgtaaaagttaacgtctcctgtttgaaggaaaattcctccaatgtagctcccaactgcattattgagaattctgttcctcctcagtcatgcgatgccttcagtattgctaataatggtgtcaatggacattatgctgatagtgttacagaggttgttgacatgaaaagcaatattgttgatgcatgcaatcaaacattgcctccagatttactgagtgtttgcacccaagagaatgatcagagtagtgataaggttaaagaatgtgtggaacagaaagttgacggtatgactgatatgagggttgaaactggcactcagatgttactaaaagaggaaaaggcctccaatctcaccaaggattctgctggattatttcccaacagtactattgagaattctgttcctctgcaactgcatcaaccttttgatattgtcagtaatgactcttctaagacggtcgatgttccagatatcaatgattctcctggtcatgttgaatctagtacttcatttgaccattctggactgatggatcatgaaggaaatgataatttcagagttgagttttttcctaaaactaatttttctgaaattattgcattgcctgctcaaaggagtggccgaagccgcaagactccgacaaaaaaggctccaaggaaacgcaggaatgcatctaaagtgttacaacctcttggaagtgttgaaagtgtcttcaaaggtgctggaaggaagagaagctgcctttccaaacctcctcgttcttctacctggggattattgggaagtgtcactcaatcctttgaggagagtaatgggcttcaggtccatcaggtttgtcaaggtcagaatgaaggatcacagaaacgaaggggtggtcgaagaagtggaaaacagaagcaaagtgtggctagtggaaattttcaagggtctaggggaacttcaactaatcatgttcgtttgaaggttaaatttgggaaagaagtcgatagaaatttactgtatgacaaagcagctgagtttgttgatacctcagcatgtgcaaattctatgcaaatagtgaatgctgttgaaggtaattggagacaagaagcaactgttaggaagtgtcagcatacaaacaagaaactagagaaggatggagaactggctaataaggacttggagactgtttctgttacagagaattcagctgaagatgagatacagaattgtcttgtggttccctctcatgcaattgctatatcatcaggaggatcagttgggagtagctatagagaccctggaacttcacctgattctgaagttatcaatttaattccagaagcacatgtggaggcaaggccacaagaagattctcatggtactgatttgacttcagacaaagttttatctgcttttggagactttattagcagtaagagaggaaagaagaaacataaactcccttatgcgggaaattgtgttccggaagatggttcaccgtgtccatgcccagctagcacaatgagagcaaaaccatcaacacgtgatggatgtagacagaatggtagtcaagatatttgctctggtgagacttttacttcatccccaggtgcaaaggcttcaactaactcatcaagtgacagggacttgtctgtggaacccctgtgcttgtcaggaggaagtgaccatggagtggagaggggtgcagaagctgagacggattgcattctagatgtgggcttaggtctgcataattcccagaatatgcttccttcctctaacaccaaggggcagaagcctcccaagggcaaattgagaggttctgacttagcgagtaagagaagtaatactcgtaaaccaagggaaaaagagcagaagtctgttaataagaaaaaagtcaaggaggacaaacagcttgcctgcaaggtggaaagtctccctgaatcaggagatcccttagcagatgccaacacttcccatgttgctgagtgcataggtgttcctaacttggacgtggtacctgttgacttagataagcagtatgtaccccctcggaatgcgtgggtgctctgtgatgcttgtaataaatggaggcgtataccagctgaacttgcagattttatagatgaaacaaagtgcacatggacctgtagagagaatcaggacagaaactttgctgattgctcaattcctcaagagaagtcaaatgcagagattaatgcagagttggaaatatctgatgcttcaggcgaagaagatgcttctggcacccgattacattataaaacattggaatgccgtcgtccgtcagtttcccaacagaatgttgcatctattaaaaccaatcagttccttcaccgtaatcgtaaaaatcagagtattgatgagataatggtttgccattgcaaactatctaaagatggccagttgggttgtggagaagattgcctgaatcgaatgcttaacatcgaatgtgttcgaggaacctgtccgtgtggagatctttgttcaaatcaacagttccaaaaacgccgatattccaaactagagaagtttcgaagtgggaagaagggatttggtctcagatcgcttgagcatatatgtaaagggcagtttcttattgaatatgttggagaggtgcttgatacacatgcttatgaggcacgacaaaaagagtacgctgtaaagggtcacagacatttctacttcatgacattgaataccagtgaggtaatagatgcatgtgcaaagggaaatttggggcgattcatcaaccatagttgtgatccgaactgtcgcaccgagaagtggatggtgaatggagaggtctgtataggactatttgcactaagagatataaagaagggtgaagaggtgacatttgactacaactttgtaagggtgattggagctgctgcaaagaaatgttattgtggttcacctcagtgtcagggttacataggtggtgatcctcttaatactgaaatcattgttcaagatgattcagatgaagaatatgtggagcctgtgatgatcccagaagattgtgtagcggaagatagtagaggcaatgcggtggccagactcgattccttagatggtgcgataatccaacgtgaggagagtgcatctgcaaataaagacattgataaatcagcaatttctgttggtaaattggagattaccacacagagagaggagtccgtggaattgcagcacttgttgccatcttttgtccaaccagttgaagtgttccaacaaacagaagatgtaactagtagatccacgcctgttgttcagcaggaggttttcagggaaaaggagactacagaaaaatcctcagactcctttgaaagaccagagattacttctccaacaaaagtgattagcaaacctttatctgatgatattgatgctaacaaaaactccaagtttaacactattgaagatgagcagctttcctctaaagtgcatcggaatgtgaaaacttctcgttcatccagttctgtgaagaaaggcaaagttaggagtactcctctaaacacaaacaaaattcaggtggtagccaataaatctcatgtgctaccttttaaaccgaaaagatcaatcgaaggatcagttgaagaaaagcttaatgagctgttggatgttgatggagggataagtaaacgcaaagattcgactaaaggctacttaaagcttctgttccttaccgcacaatcaggtgatagtggtagtggggaagcaattaaaagcaatcgagatctttctattattcttgatgcacttttgaagacaaagtcgcgtacggttttgattgatattattaacaaaaatgggttgagaatgctacacaacattatgaagatgtgcaggagagactttaacaagattccgatcctccggaagcttttgaaggtcctagaatatttggccgaaaagccacagattcttacacaggaacatattactggaggtcctccctgcccgggaatggagagctttacagagtcgatactatcactgacggagcatggtgacaaaagggtccatgacatagcacgcaacttccgaaataggtggattccaaaagctttcagaagacatagttttgtagacagggatgatggaaagatggaatttaacaggagttcaaactacaataggttttccacaacacatgataattggcgtgatcaaagtggaagatctactgaagttgcagataatgtgaagcaatcagtggttaaaacaccaacttctgcgagtacggttatccaggatggctcttctacaccttctacaggtggttgtgcaaccagtgagacaaaagttcgcaagcgtaaaagccgatgggatcagccagcagtggcagtccctgatccaagttcacttccaaacaaggaacagaagatagagtctaaacagctagaatctagtccattacctcggataggtgatgtgacatcgcaccctgaaaaggggagcagagagggcggaaattgcaccagcactgtgcatgatttatgtcagcaagttggtgctgatgtggttaatgatggaaagcaaaacattcctgaggatgttcctcctggattttcatcttgcattaatacccctgtggtttcgtcaacatcttcagttatagggcatccgcaggccaagtttgtttctcgcttacctgtctcgtatggaattccactgtctatcatgcagcaatatgggacaccccatgctgaaagtgttgatacttgggttgttgctcctggcatgcctttccatccttttccaccattgcccccatgtccccgtcataagaaagacccttctcatgatgtcaatcatttgtcagttaaccaagcttcagaaggacaacaagccagctgtgttgctacgaattgtcactctgaagaaagcagtccaagcacaactggtgccgctcaagcagactttggcacacaatctgcaaataatcaattgggtactaaacgacagagggagtcctcatatgaggcccccttggggaggagatacttcaagcagcagaagtggaatcatccaaaaccgaggctcccatgggttagggataggaatggttggggatgtaatgggaacaacttcagaggtgggacaaacagcataggcgtaggaactgtaggaaatgagcttagaactccatattgttcagaggacatcaactatagggtggagaaagctggcaataatgtcaatcagcattcacatcaccattaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001763 GO:0002682 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006464 GO:0006479 GO:0006629 GO:0006720 GO:0006721 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008213 GO:0008276 GO:0008757 GO:0009553 GO:0009555 GO:0009653 GO:0009791 GO:0009893 GO:0009908 GO:0009909 GO:0009910 GO:0009987 GO:0010016 GO:0010223 GO:0010346 GO:0010363 GO:0010452 GO:0010468 GO:0010604 GO:0010638 GO:0010941 GO:0016043 GO:0016108 GO:0016116 GO:0016278 GO:0016279 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018022 GO:0018024 GO:0018193 GO:0018205 GO:0019222 GO:0019538 GO:0022414 GO:0031056 GO:0031058 GO:0031060 GO:0031062 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0032259 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033043 GO:0033044 GO:0034968 GO:0035670 GO:0036211 GO:0040029 GO:0042054 GO:0042800 GO:0043067 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044255 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045088 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048443 GO:0048466 GO:0048467 GO:0048481 GO:0048518 GO:0048519 GO:0048522 GO:0048580 GO:0048581 GO:0048583 GO:0048608 GO:0048646 GO:0048653 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0050776 GO:0050789 GO:0050793 GO:0050794 GO:0051093 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051239 GO:0051241 GO:0051246 GO:0051247 GO:0051276 GO:0051568 GO:0060255 GO:0061458 GO:0065007 GO:0071704 GO:0071840 GO:0080090 GO:0080134 GO:0080135 GO:0090567 GO:0099402 GO:0140096 GO:1901564 GO:1902275 GO:1905269 GO:1905393 GO:2000026 GO:2000241 GO:2000242 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko00310 map00310
Pfam Domains
Protein Families
SET zf-CW

Protein Analysis

2106

Amino Acids

230.62

Weight (kDa)

5.68

Isoelectric Point (pI)

48.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3351
AarI CACCTGC 1 cut(s) 736
AasI GACNNNNNNGTC 1 cut(s) 5114
Acc36I ACCTGC 1 cut(s) 736
Acc65I GGTACC 2 cut(s) 56, 3085
AccB1I GGYRCC 4 cut(s) 56, 3085, 3335, 6002
AccB7I CCANNNNNTGG 2 cut(s) 497, 2761
AccBSI CCGCTC 1 cut(s) 6008
AccI GTMKAC 4 cut(s) 609, 2623, 3166, 5184
AccII CGCG 1 cut(s) 4873
AccIII TCCGGA 2 cut(s) 2551, 4971
AcoI YGGCCR 4 cut(s) 1705, 3490, 4146, 5001
AcvI CACGTG 1 cut(s) 2612
AflIII ACRYGT 3 cut(s) 171, 2413, 2609
AhlI ACTAGT 1 cut(s) 4343
AjiI CACGTC 2 cut(s) 172, 3082
AjnI CCWGG 9 cut(s) 136, 490, 1570, 1853, 2365, 2674, 5346, 5659, 5833
AjuI GAANNNNNNNTTGG 2 cut(s) 101, 133
AleI CACNNNNGTG 1 cut(s) 4012
AloI GAACNNNNNNTCC 2 cut(s) 2532, 2564
Alw21I GWGCWC 3 cut(s) 519, 3138, 3209
Alw44I GTGCAC 1 cut(s) 3205
AlwNI CAGNNNCTG 2 cut(s) 2780, 5447
Ama87I CYCGRG 1 cut(s) 5056
Aor13HI TCCGGA 2 cut(s) 2551, 4971
AoxI GGCC 8 cut(s) 1425, 1705, 2426, 3490, 4146, 5001, 5727, 6081
ApaLI GTGCAC 1 cut(s) 3205
ArsI GACNNNNNNTTYG 6 cut(s) 1945, 1977, 3224, 3256, 3944, 3976
AseI ATTAAT 2 cut(s) 3285, 5679
Asp700I GAANNNNTTC 3 cut(s) 748, 3543, 6185
Asp718I GGTACC 2 cut(s) 56, 3085
AspS9I GGNCC 7 cut(s) 151, 1909, 3213, 4987, 5045, 5116, 6082
AsuC2I CCSGG 3 cut(s) 180, 5057, 5058
AsuII TTCGAA 1 cut(s) 3625
AsuNHI GCTAGC 1 cut(s) 2582
AvaI CYCGRG 1 cut(s) 5056
AvaII GGWCC 6 cut(s) 151, 1909, 3213, 4987, 5045, 5116
AxyI CCTNAGG 1 cut(s) 5646
BaeGI GKGCMC 2 cut(s) 462, 3209
BaeI ACNNNNGTAYC 2 cut(s) 5808, 5841
BalI TGGCCA 2 cut(s) 3492, 4148
BanI GGYRCC 4 cut(s) 56, 3085, 3335, 6002
BanII GRGCYC 2 cut(s) 77, 843
BarI GAAGNNNNNNTAC 4 cut(s) 1516, 1548, 2271, 2303
BbrPI CACGTG 1 cut(s) 2612
BbsI GAAGAC 4 cut(s) 543, 1789, 4868, 5176
Bbv12I GWGCWC 3 cut(s) 519, 3138, 3209
BbvCI CCTCAGC 1 cut(s) 2116
BceAI ACGGC 2 cut(s) 877, 3351
BcgI CGANNNNNNTGC 2 cut(s) 3308, 3342
BciT130I CCWGG 9 cut(s) 138, 492, 1572, 1855, 2367, 2676, 5348, 5661, 5835
BclI TGATCA 2 cut(s) 1321, 5263
BcnI CCSGG 3 cut(s) 180, 5057, 5058
BcuI ACTAGT 1 cut(s) 4343
BfmI CTRYAG 7 cut(s) 217, 2356, 3217, 4395, 5370, 6222, 6268
BfuAI ACCTGC 1 cut(s) 736
BglII AGATCT 4 cut(s) 81, 3568, 4831, 5276
BlpI GCTNAGC 1 cut(s) 126
BmcAI AGTACT 3 cut(s) 1471, 1591, 4600
Bme18I GGWCC 6 cut(s) 151, 1909, 3213, 4987, 5045, 5116
BmeT110I CYCGRG 1 cut(s) 5056
BmgBI CACGTC 2 cut(s) 172, 3082
BmgT120I GGNCC 7 cut(s) 151, 1909, 3213, 4987, 5045, 5116, 6082
BmrI ACTGGG 1 cut(s) 699
BmtI GCTAGC 1 cut(s) 2586
BmuI ACTGGG 1 cut(s) 699
BpiI GAAGAC 4 cut(s) 543, 1789, 4868, 5176
BplI GAGNNNNNCTC 2 cut(s) 4588, 4620
BpmI CTGGAG 2 cut(s) 1272, 5061
Bpu10I CCTNAGC 3 cut(s) 2116, 2809, 3025
Bpu1102I GCTNAGC 1 cut(s) 126
Bpu14I TTCGAA 1 cut(s) 3625
BpuEI CTTGAG 3 cut(s) 3246, 3680, 5994
BpuMI CCSGG 3 cut(s) 180, 5057, 5058
BsaAI YACGTR 1 cut(s) 2612
BsaBI GATNNNNATC 4 cut(s) 348, 4964, 5668, 5907
BsaI GGTCTC 2 cut(s) 2355, 3653
BsaWI WCCGGW 2 cut(s) 2551, 4971
Bse21I CCTNAGG 1 cut(s) 5646
Bse3DI GCAATG 5 cut(s) 1280, 1685, 3469, 4144, 5861
Bse8I GATNNNNATC 4 cut(s) 348, 4964, 5668, 5907
BseAI TCCGGA 2 cut(s) 2551, 4971
BseBI CCWGG 9 cut(s) 138, 492, 1572, 1855, 2367, 2676, 5348, 5661, 5835
BseJI GATNNNNATC 4 cut(s) 348, 4964, 5668, 5907
BseMI GCAATG 5 cut(s) 1280, 1685, 3469, 4144, 5861
BseRI GAGGAG 7 cut(s) 1155, 1830, 1904, 4202, 4280, 4593, 6109
BseSI GKGCMC 2 cut(s) 462, 3209
BseYI CCCAGC 2 cut(s) 957, 2578
BsgI GTGCAG 3 cut(s) 714, 2793, 4960
Bsh1236I CGCG 1 cut(s) 4873
Bsh1285I CGRYCG 1 cut(s) 1545
BshFI GGCC 8 cut(s) 1427, 1707, 2428, 3492, 4148, 5003, 5729, 6083
BshNI GGYRCC 4 cut(s) 56, 3085, 3335, 6002
BsiEI CGRYCG 1 cut(s) 1545
BsiHKAI GWGCWC 3 cut(s) 519, 3138, 3209
BsiHKCI CYCGRG 1 cut(s) 5056
BsiSI CCGG 5 cut(s) 179, 296, 2552, 4972, 5057
BsiWI CGTACG 1 cut(s) 4874
BslFI GGGAC 6 cut(s) 733, 2726, 5429, 5806, 5859, 6211
BsmBI CGTCTC 2 cut(s) 1105, 2777
BsmFI GGGAC 6 cut(s) 733, 2726, 5429, 5806, 5859, 6211
BsmI GAATGC 9 cut(s) 750, 1759, 2152, 2793, 3130, 3368, 3531, 4920, 6304
BsnI GGCC 8 cut(s) 1427, 1707, 2428, 3492, 4148, 5003, 5729, 6083
Bso31I GGTCTC 2 cut(s) 2355, 3653
BsoBI CYCGRG 1 cut(s) 5056
Bsp119I TTCGAA 1 cut(s) 3625
Bsp1286I GDGCHC 6 cut(s) 77, 462, 519, 843, 3138, 3209
Bsp13I TCCGGA 2 cut(s) 2551, 4971
Bsp1720I GCTNAGC 1 cut(s) 126
Bsp19I CCATGG 2 cut(s) 2755, 6143
BspANI GGCC 8 cut(s) 1427, 1707, 2428, 3492, 4148, 5003, 5729, 6083
BspEI TCCGGA 2 cut(s) 2551, 4971
BspFNI CGCG 1 cut(s) 4873
BspHI TCATGA 3 cut(s) 1621, 3777, 5899
BspMAI CTGCAG 1 cut(s) 221
BspMI ACCTGC 1 cut(s) 736
BspOI GCTAGC 1 cut(s) 2586
BspQI GCTCTTC 2 cut(s) 573, 5362
BspT104I TTCGAA 1 cut(s) 3625
BspT107I GGYRCC 4 cut(s) 56, 3085, 3335, 6002
BspTNI GGTCTC 2 cut(s) 2355, 3653
BsrBI CCGCTC 1 cut(s) 6008
BsrDI GCAATG 5 cut(s) 1280, 1685, 3469, 4144, 5861
BssNAI GTATAC 1 cut(s) 3167
Bst1107I GTATAC 1 cut(s) 3167
Bst2UI CCWGG 9 cut(s) 138, 492, 1572, 1855, 2367, 2676, 5348, 5661, 5835
Bst6I CTCTTC 6 cut(s) 573, 1023, 1537, 1811, 3933, 5362
BstAPI GCANNNNNTGC 2 cut(s) 1691, 4291
BstBAI YACGTR 1 cut(s) 2612
BstBI TTCGAA 1 cut(s) 3625
BstDSI CCRYGG 4 cut(s) 715, 2755, 4272, 6143
BstEII GGTNACC 1 cut(s) 486
BstENI CCTNNNNNAGG 5 cut(s) 480, 3012, 5370, 5520, 6091
BstFNI CGCG 1 cut(s) 4873
BstMCI CGRYCG 1 cut(s) 1545
BstNI CCWGG 9 cut(s) 138, 492, 1572, 1855, 2367, 2676, 5348, 5661, 5835
BstNSI RCATGY 8 cut(s) 879, 934, 1272, 2124, 2417, 3722, 3813, 5842
BstPI GGTNACC 1 cut(s) 486
BstSFI CTRYAG 7 cut(s) 217, 2356, 3217, 4395, 5370, 6222, 6268
BstSLI GKGCMC 2 cut(s) 462, 3209
BstUI CGCG 1 cut(s) 4873
BstV2I GAAGAC 4 cut(s) 543, 1789, 4868, 5176
BstX2I RGATCY 7 cut(s) 81, 911, 3019, 3568, 4349, 4831, 5276
BstYI RGATCY 7 cut(s) 81, 911, 3019, 3568, 4349, 4831, 5276
BstZ17I GTATAC 1 cut(s) 3167
Bsu36I CCTNAGG 1 cut(s) 5646
BsuRI GGCC 8 cut(s) 1427, 1707, 2428, 3492, 4148, 5003, 5729, 6083
BtgI CCRYGG 4 cut(s) 715, 2755, 4272, 6143
BtgZI GCGATG 2 cut(s) 1191, 5520
BtrI CACGTC 2 cut(s) 172, 3082
BtsI GCAGTG 2 cut(s) 226, 5442
BveI ACCTGC 1 cut(s) 736
CaiI CAGNNNCTG 2 cut(s) 2780, 5447
CciI TCATGA 3 cut(s) 1621, 3777, 5899
Cfr13I GGNCC 7 cut(s) 151, 1909, 3213, 4987, 5045, 5116, 6082
Cfr9I CCCGGG 1 cut(s) 5056
CsiI ACCWGGT 1 cut(s) 136
CspCI CAANNNNNGTGG 4 cut(s) 316, 351, 4342, 4377
DraI TTTAAA 1 cut(s) 4660
DrdI GACNNNNNNGTC 1 cut(s) 5114
DseDI GACNNNNNNGTC 1 cut(s) 5114
EaeI YGGCCR 4 cut(s) 1705, 3490, 4146, 5001
Eam1104I CTCTTC 6 cut(s) 573, 1023, 1537, 1811, 3933, 5362
EarI CTCTTC 6 cut(s) 573, 1023, 1537, 1811, 3933, 5362
EciI GGCGGA 2 cut(s) 182, 5580
Eco147I AGGCCT 1 cut(s) 1427
Eco24I GRGCYC 2 cut(s) 77, 843
Eco31I GGTCTC 2 cut(s) 2355, 3653
Eco32I GATATC 2 cut(s) 1080, 1558
Eco47I GGWCC 6 cut(s) 151, 1909, 3213, 4987, 5045, 5116
Eco72I CACGTG 1 cut(s) 2612
Eco81I CCTNAGG 1 cut(s) 5646
Eco88I CYCGRG 1 cut(s) 5056
Eco91I GGTNACC 1 cut(s) 486
EcoNI CCTNNNNNAGG 5 cut(s) 480, 3012, 5370, 5520, 6091
EcoO109I RGGNCCY 3 cut(s) 4987, 5045, 6082
EcoO65I GGTNACC 1 cut(s) 486
EcoRI GAATTC 4 cut(s) 1153, 1480, 2269, 5763
EcoRII CCWGG 9 cut(s) 136, 490, 1570, 1853, 2365, 2674, 5346, 5659, 5833
EcoRV GATATC 2 cut(s) 1080, 1558
EcoT22I ATGCAT 3 cut(s) 1270, 1759, 3811
EcoT38I GRGCYC 2 cut(s) 77, 843
Esp3I CGTCTC 2 cut(s) 1105, 2777
FalI AAGNNNNNCTT 2 cut(s) 4683, 4715
FaqI GGGAC 6 cut(s) 733, 2726, 5429, 5806, 5859, 6211
FauI CCCGC 4 cut(s) 256, 383, 830, 2530
FauNDI CATATG 1 cut(s) 6076
FbaI TGATCA 2 cut(s) 1321, 5263
FblI GTMKAC 4 cut(s) 609, 2623, 3166, 5184
FriOI GRGCYC 2 cut(s) 77, 843
GsaI CCCAGC 2 cut(s) 961, 2582
GsuI CTGGAG 2 cut(s) 1272, 5061
HaeIII GGCC 8 cut(s) 1427, 1707, 2428, 3492, 4148, 5003, 5729, 6083
HapII CCGG 5 cut(s) 179, 296, 2552, 4972, 5057
HincII GTYRAC 6 cut(s) 1096, 1243, 1366, 3094, 5700, 5923
HindII GTYRAC 6 cut(s) 1096, 1243, 1366, 3094, 5700, 5923
HindIII AAGCTT 5 cut(s) 4697, 4766, 4976, 5160, 5928
HpaI GTTAAC 2 cut(s) 1096, 5923
HpaII CCGG 5 cut(s) 179, 296, 2552, 4972, 5057
Hpy99I CGWCG 1 cut(s) 3372
HpyCH4IV ACGT 5 cut(s) 171, 1098, 2611, 3081, 4183
HpySE526I ACGT 5 cut(s) 171, 1098, 2611, 3081, 4183
Kpn2I TCCGGA 2 cut(s) 2551, 4971
KpnI GGTACC 2 cut(s) 60, 3089
Ksp22I TGATCA 2 cut(s) 1321, 5263
KspAI GTTAAC 2 cut(s) 1096, 5923
LguI GCTCTTC 2 cut(s) 573, 5362
LmnI GCTCC 9 cut(s) 292, 1138, 1741, 3977, 4090, 5098, 5553, 5836, 6144
MabI ACCWGGT 1 cut(s) 136
MaeII ACGT 5 cut(s) 171, 1098, 2611, 3081, 4183
MbiI CCGCTC 1 cut(s) 6008
MfeI CAATTG 2 cut(s) 2319, 6044
MflI RGATCY 7 cut(s) 81, 911, 3019, 3568, 4349, 4831, 5276
MhlI GDGCHC 6 cut(s) 77, 462, 519, 843, 3138, 3209
MlsI TGGCCA 2 cut(s) 3492, 4148
MluNI TGGCCA 2 cut(s) 3492, 4148
MlyI GAGTC 9 cut(s) 56, 1523, 1714, 4146, 4277, 4406, 5087, 5495, 6077
MmeI TCCRAC 8 cut(s) 1748, 3276, 3679, 4204, 4330, 4348, 4466, 4692
Mox20I TGGCCA 2 cut(s) 3492, 4148
Mph1103I ATGCAT 3 cut(s) 1270, 1759, 3811
MroI TCCGGA 2 cut(s) 2551, 4971
MroXI GAANNNNTTC 3 cut(s) 748, 3543, 6185
MscI TGGCCA 2 cut(s) 3492, 4148
MslI CAYNNNNRTG 7 cut(s) 522, 1403, 3361, 3723, 3849, 4012, 4545
Msp20I TGGCCA 2 cut(s) 3492, 4148
MspA1I CMGCKG 4 cut(s) 2099, 2276, 3173, 5952
MspI CCGG 5 cut(s) 179, 296, 2552, 4972, 5057
MunI CAATTG 2 cut(s) 2319, 6044
Mva1269I GAATGC 9 cut(s) 750, 1759, 2152, 2793, 3130, 3368, 3531, 4920, 6304
MvaI CCWGG 9 cut(s) 138, 492, 1572, 1855, 2367, 2676, 5348, 5661, 5835
MvnI CGCG 1 cut(s) 4873
NciI CCSGG 3 cut(s) 180, 5057, 5058
NcoI CCATGG 2 cut(s) 2755, 6143
NdeI CATATG 1 cut(s) 6076
NheI GCTAGC 1 cut(s) 2582
NsiI ATGCAT 3 cut(s) 1270, 1759, 3811
NspI RCATGY 8 cut(s) 879, 934, 1272, 2124, 2417, 3722, 3813, 5842
NspV TTCGAA 1 cut(s) 3625
OliI CACNNNNGTG 1 cut(s) 4012
PaeI GCATGC 3 cut(s) 879, 1272, 5842
PagI TCATGA 3 cut(s) 1621, 3777, 5899
PaqCI CACCTGC 1 cut(s) 736
PceI AGGCCT 1 cut(s) 1427
PciI ACATGT 1 cut(s) 2413
PciSI GCTCTTC 2 cut(s) 573, 5362
PcsI WCGNNNNNNNCGW 1 cut(s) 5137
PctI GAATGC 9 cut(s) 750, 1759, 2152, 2793, 3130, 3368, 3531, 4920, 6304
PdmI GAANNNNTTC 3 cut(s) 748, 3543, 6185
Pfl23II CGTACG 1 cut(s) 4874
PflFI GACNNNGTC 1 cut(s) 4867
PflMI CCANNNNNTGG 2 cut(s) 497, 2761
PfoI TCCNGGA 2 cut(s) 5346, 5659
PleI GAGTC 9 cut(s) 55, 1523, 1714, 4146, 4276, 4406, 5086, 5494, 6076
PmaCI CACGTG 1 cut(s) 2612
PmlI CACGTG 1 cut(s) 2612
PpsI GAGTC 9 cut(s) 55, 1523, 1714, 4146, 4276, 4406, 5086, 5494, 6076
Ppu21I YACGTR 1 cut(s) 2612
PpuMI RGGWCCY 2 cut(s) 4987, 5045
PscI ACATGT 1 cut(s) 2413
PshBI ATTAAT 2 cut(s) 3285, 5679
PsiI TTATAA 1 cut(s) 3351
Psp5II RGGWCCY 2 cut(s) 4987, 5045
Psp6I CCWGG 9 cut(s) 136, 490, 1570, 1853, 2365, 2674, 5346, 5659, 5833
PspCI CACGTG 1 cut(s) 2612
PspEI GGTNACC 1 cut(s) 486
PspFI CCCAGC 2 cut(s) 957, 2578
PspGI CCWGG 9 cut(s) 136, 490, 1570, 1853, 2365, 2674, 5346, 5659, 5833
PspLI CGTACG 1 cut(s) 4874
PspPI GGNCC 7 cut(s) 151, 1909, 3213, 4987, 5045, 5116, 6082
PspPPI RGGWCCY 2 cut(s) 4987, 5045
PstI CTGCAG 1 cut(s) 221
PstNI CAGNNNCTG 2 cut(s) 2780, 5447
PsuI RGATCY 7 cut(s) 81, 911, 3019, 3568, 4349, 4831, 5276
PsyI GACNNNGTC 1 cut(s) 4867
PvuII CAGCTG 4 cut(s) 2099, 2276, 3173, 5952
RseI CAYNNNNRTG 7 cut(s) 522, 1403, 3361, 3723, 3849, 4012, 4545
SapI GCTCTTC 2 cut(s) 573, 5362
Sau96I GGNCC 7 cut(s) 151, 1909, 3213, 4987, 5045, 5116, 6082
ScaI AGTACT 3 cut(s) 1471, 1591, 4600
SchI GAGTC 9 cut(s) 56, 1523, 1714, 4146, 4277, 4406, 5087, 5495, 6077
SduI GDGCHC 6 cut(s) 77, 462, 519, 843, 3138, 3209
SexAI ACCWGGT 1 cut(s) 136
SfcI CTRYAG 7 cut(s) 217, 2356, 3217, 4395, 5370, 6222, 6268
SfuI TTCGAA 1 cut(s) 3625
SinI GGWCC 6 cut(s) 151, 1909, 3213, 4987, 5045, 5116
SmaI CCCGGG 1 cut(s) 5058
SmiMI CAYNNNNRTG 7 cut(s) 522, 1403, 3361, 3723, 3849, 4012, 4545
SmlI CTYRAG 3 cut(s) 3261, 3659, 6009
SmoI CTYRAG 3 cut(s) 3261, 3659, 6009
SpeI ACTAGT 1 cut(s) 4343
SphI GCATGC 3 cut(s) 879, 1272, 5842
SseBI AGGCCT 1 cut(s) 1427
SspI AATATT 2 cut(s) 1258, 4997
StuI AGGCCT 1 cut(s) 1427
TaiI ACGT 5 cut(s) 174, 1101, 2614, 3084, 4186
TatI WGTACW 3 cut(s) 1469, 1589, 4598
TauI GCSGC 2 cut(s) 1716, 6008
TspGWI ACGGA 6 cut(s) 245, 2801, 3363, 3549, 4261, 5111
TspMI CCCGGG 1 cut(s) 5056
Tth111I GACNNNGTC 1 cut(s) 4867
Van91I CCANNNNNTGG 2 cut(s) 497, 2761
VneI GTGCAC 1 cut(s) 3205
VpaK11BI GGWCC 6 cut(s) 151, 1909, 3213, 4987, 5045, 5116
VspI ATTAAT 2 cut(s) 3285, 5679
XagI CCTNNNNNAGG 5 cut(s) 480, 3012, 5370, 5520, 6091
XbaI TCTAGA 1 cut(s) 2797
XceI RCATGY 8 cut(s) 879, 934, 1272, 2124, 2417, 3722, 3813, 5842
XcmI CCANNNNNNNNNTGG 1 cut(s) 3500
XmaI CCCGGG 1 cut(s) 5056
XmiI GTMKAC 4 cut(s) 609, 2623, 3166, 5184
XmnI GAANNNNTTC 3 cut(s) 748, 3543, 6185
ZrmI AGTACT 3 cut(s) 1471, 1591, 4600
Zsp2I ATGCAT 3 cut(s) 1270, 1759, 3811
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.