MD15G1133700.v1.1

Histone-lysine N-methyltransferase ASHH2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0007450
Physical Location & Seq
Reverse (-)
9701529 .. 9713384
11856 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1133700.v1.1.491

Sequence Viewer

Length: 6369 bp
ATGGGCTCAGAGATCTTGGTGCAATCTGTTTCTGAGCAGCCATCCTGCTCGGAGCCTTTACTTGATTCTGATGTTGACCATGCCAGTGTGCCAGATGGGGCTCTGGGATTGTCTAGGAATGATAATGCTGGTTTTGTGATCTCTGGCGAGGTTACAGAGTCATGCAAGGTTGATAGAGATGGTTTCGTTGGTGAGCATGAGAATGCCAATGATTTGGATCTGCGAAGTAGGTTGAACGGAGATTGGAGGCTCAATAGGTGTTGCTCGACTGAGAGTCAGAGTAATGTTGATGGTTCCAATAGGGAAAGTGATGGGACATGCTTGAAGGAAGGTGGGCCTGAACTTGAGGATGGATCTGCAGCTTCAGTTGGGGACTGTGACATCCCTTTGGAAGTCATATGTCAGATTGGTTCATCAGGAGACGGTGTCCAACAGGATGAGCTGAGAGATGATAAGAGTGTTAGTTGTCTCTATTCTAAAGAGGTTATGGAAGTTACGGAAGAGAAAGGTGACATTTCTCTTGAGATTAAGAATGATAATTGTGAACCAGTATCTCCTTTGCAGGGTGGTGAAATTCCCTCAGAACTAGCAGAGATGGAAGCTTGCAGTAGTCCCCATACAGAAGAGAAGGGTTTCAACATCTTAGCGGGTCCCTCTTTGGAAGCTGCATATGAGGAGAGGGTTGTTTCTGGTGGGATAGAAGCTGATTTGTGCAACAAGACATCACCTGTACGGGATGTTGGGATGCATTCAGAAGTATCATACGCATGTGGTTTAGTGACCAATTGTGACAATAAGAATGAACAGATGGATGATAATGGTGTTAATGGTCCAAGCAGCCTCTCTACTGAAAGGACTACAGAGGTTGTTGAGATGAAAAGTAATGTTGATACATGCACTCAAATATTTCCTTCGCAAGGTTTGGAGAGAGCCTTGGAAGATTTACATATGCCTGATTTACCGAGTAGTTGTGCCCAACAGAATGATCAGAGTAGTGATAAAATTGCTGATGGTTCCTTGGTAGAGAGGTCTATAGATATTCTGGAAAAAAACAGTGATTCTATGACAGATTTAGAGGCTGTCATTCTCACCCAGATGTCTCCTGTAAAAGTTAATGTCTACTATTCTAAGGAAGGTCCCTCCGAAATAGCCTCCAGCTGCATTATTGAGAAGTCTGTTTCTATGCAGTCATGTCAAACCTTAGGGGTTGCCAATAACAGCGTCAGTGGGCTCTCTACTGATATGGTTGTTGATATGAAAAGCAATACTAATCAAATATGGCCTCCAGAGGGTTGTGAGAGGGCCTTTGAAGGTTCAGATGTGTCAGATTCACTGAGGGTTTGCAGCCAAGAGAATGGTCAGAGGAACGATAAGGTTATCGATTTTGCGTTTGCTGAAAGGGTAACAGACATTTTGGAAAAGAAAAGTGATGCTACAACCGACACGAAGGTTGAAATTGGCACCCAGATCTCAGTAATGGAGGAAAAAGTCTCCAATCTGAATGAAGGTTCTGGTGGACTATCCCCCAACAATATTCATGAGAAGTCCGTTTCTTTGAAACCGTTTCAACCTTTTGATATTGTCAAAAATGGCCCTTCTGAGAGTGTGGATGTGCCAGATAATAATGATTCCCCTGGTAATATTGATTCTAGCATTTCGTTGGACTGTTCTGGAGAGAAATATCATGAAGGAATTGATAATGTCAAAGTTGGTTGTGTTTCTAAAACCAAATTTCCTGACATTGTAGCATTGTCTTCTCGAAGGAGTGGCCGAAGCCGCAAGACTAACACAAAAAGGGCTCCAAGGAAAGGCAGAAGCACATCTAAAGAGTTGCAACCACTTGGAAGCCTTGAAACTGTCTTCAAGGCTGCTGGAAGGAAGAGAAGCTGCCTTTCCAAACCAGCTCGATCTTCTGTATGGGGATTATTAGGGAGTGTAACACAATCTTTTGAAGAGAGTAACAGGCTTGAGGTCAGTCAAGGTAAGAGTCAAGTATCACAGAAACGAAGGAGTGGACAAAGAAGTGGACAACGGAATCAGAGTGGTGCTGGTGGAAATTCACAAGGGTCAAGGGGCAAGAACCGTGCGTCAACTAACCGTGTTCGTTTGAAGGTTAAAGTGGGGAAAGAATTTTGTAATAGTTCTCTCTACATCACAGTACCTGAGGATGTTGATACCTCAGCATCTGCAAATTCTGTTAAAAAAGGGGATGGAAATGAGGGCAATTGGAGAAAAGAAGCTACTGTTGGGGAGGATAGAACTTATCTGGATGCTCCTGTTCTGGATGTTGATCTGGCTAATAAGGACTTTGAGAGTGTTGTTCTCACAGAGAATTCAGCTGACAATGTGATTGATAATTATCGTACGGTTCCATCTCATACAGTTGCTGTATCTTCTGGAGGATCATTTGGGACTAGCTTTACAGATCCTGGAACTTCACCTGATTCTGAAATTATCAATATCGTTCCAGATGCAGATGTTGAGGCAAGACAGCAAGAAGATTCTCATGGTGATGTTTTAACTTCTGACAAGGTTTTGGCTGCTTCTGGAGACTTTATTAGCACTAAGAGAGGGAATGAGAAGCATAAAGTCCCTCATGCAAAAAATTATGTTTGGGAAGGTGGTAAACTTTGTCCAGCAAGCTTGAATAAAGAAATGCAATCAGAACGAGATGGATGCAGGCAAAGTCTGGATTTTTCTTCTAGTGAGATGCGCACTTCATCCACCTGTGCAAATGCTTCGAGCAACTCATCAAGTGACAAGGAATCATCTTTGGAGGCACTGCACTTGTCAGGAGAAACTGACCCTGGAATTTCTGGAGATGTTCTTAAAGTGGAAGTGGGTGCAGATAATCTTGACGTGGGCTTAGGATTATCAAAATCACAATCCTCAAAATCTAAGGGACTGAAGCCTCCCAAGGGCAGGTCCAGAGCCTGTGGCTCAGCGAGCAAGAAGGGTAATGCTCATAGACTGACGGAAAACCGGAAAAAGTCCGCTAACAAGAAGAAAGTCATGGAAAAGGCTGATGGTGATCAGGTTTCATGCGAAGTGGCAAATCTCCCAGAATCAGGCAATCACTTAGTGGACAACAATAGAAAAACCAACTCTGTTAATGATGCTGAATGTGTAGGCGTTCCTAACTCGGACATGGTACCTGTTAACATAGATAAGCAGTATTTACCACCGCGTAATGCTTGGGTGCTCTGTGATGATTGTCATAAATGGAGGCGTATACCAGCTGAACTTGCAGACATTATAGATGAACAAAAGTGCACATGGACCTGTAAAGATAACAAGGATGAAGCCTTTGCTGATTGCTCAATCCGTCAAGAGAAGTCAAATTCAGAGATTAATGCGGAGTTGGATATATCAGATGCCTCAGGTGATGAAGATGCATCCGTCACCCGATCAAAGTACAAAGAATTGGAATGTCGACGTCCAACAGTTTCCCAACAGAATGTTGCAAGCATAAAAATTAATCAGTTCCATCACCGTAGCCGTAAAACTCAGAATATTGATGAGATAATGGTTTGCCATTGCAAACCGCCTTCAGATGGTCAGTTGGGTTGTGGAGATGATTGCCTGAATCGAATACTTAATATTGAATGTGTTAGAGGAGCCTGTCCATGTAGAGACCTCTGTTCAAATCAGCAGTTCCAAAAACGCCGATACGCCAAACTAGAGAAGTTTCGAAGTGGAAAGAAGGGTTTTGGCCTCAGGTTGCTTGAGGATATATGTAAAGGGCAATTTCTTATTGAATATGTTGGAGAGGTGCTTGATACAAATGCTTACGAGGCACGACAAAAGGAGTATGCTGTGAAGGGTCACAGACATTTCTACTTCATGACATTGAATGGCAGTGAGGTAATAGATGCATGTGCAAAGGGGAATTTGGGGCGTTTCATTAACCATAGTTGTGATCCGAATTGTCGCACAGAAAAGTGGATGGTGAATGGAGAGATTTGTATAGGACTATTTGCATTGAGAGATATAAAGAAGGGTGAAGAGGTTACATTTGACTACAACTATGTAAGGGTTTTCGGAGCTGCTGCCAAAAAATGTCATTGTGGTTCATCTCAATGTCGGGGTTACATAGGTGGTGATCCTCTTGATACCGAAGTCATTGTTCAAGATGATTCAGATGAAGAATATATGGAGCCTGTGATGATCCCAGAAGATGGTGTATCTGAAGATAGGTCTGACAATACGTTGCCTGCAAATAAAGTAATTGATAATACAACAGTTTCTATTGGGGAATTGGAGTTCACCACACAAAGAGAGGAGTCCATGAATCGATCTGATTCACTTGTTTCTCACTTACACGATTCATTGGAATTGAAGCACCCAAGACAGAAACCATCTTCTGTCCCACCAGTTGAAACAGAAGATGTAGCTAGTATACCCATACCTGCTATTGAGCAGGAAATTTTTGGGGAAAAGGAGACTACAGAAAAATCCTCAAACTCCTCTGAAAGACTAGAGACTACTCCAGTAAAACTGCTTGGCAAATCGTTATCTGATGGCACTGACAGTAACAGGAAGTCCAAGTCTGGTACTATTGAAGTTGGACAGGTTCCTTCCAAAGTGTGTTCTAATGTGAAAACTTCCAAATCAACCAGTTTTGTGAAGAAAAGCAAAGTGAAGATTACCCCAAGTGGAAACAAAATTCAAATGGCAGCCACCAAATCTCTCGTGCTATCTATCAAGCCCAAACGATTAACAGAAGGTTCAGTTGAGGAGAAGCTTAACGAGCTGTTGGATGGCGACGGAGGGATAAATAAACGGAAAGATTCCACTAAAGGCTACTTGAAGCTTCTGATCCTTACCGCTGTATCTGGTGATAGTGGCAGTGGTGAAGTAATTCAGAGCAATCGAGATCTTTCTATGATCCTTGATGCGCTTTTGAAAACAAAGTCACGGATGGTTTTGATTGATGTAATAAATAAAAACGGTTTGAGAATGCTACACAACATAATGAAAAAATACAGAGAAGACTTCAAAAAGATACCAATCCTCCGGAAGCTTCTGAAGGTCCTAGAGTATTTAGCTATGAGGCATATACTTACATCAGAGCATATTACTGGAGGTCCTCCCTGCGCTGGAATGGAGAGCTTTATGGAGTCAATGCTGTCACTGACAGAACACGAAGACAGACAGGTCCACCAAATAGCACGGAATTTTCGAGACAAGTGGATTCCCAGACCTTTCAGAAGACATGGCTATGTAGACAGGGTTGATAGCAAGATGGAATTTAACAGAGGTTCAAACTCCAACAGATTTTCCCCATCACATGATAATTGGCGTGATCAAAGTGGAAGGTCTACTGAAGCTATAGATAGTGCCAAACAATCAATGGTTTCAACTTCTGTGAGTACTGGCGTCCAGGAGGTCTCTTCTGCACCTTGTACGGGTGGTGGCCCTACCAGTGAGACAAAAGTTCGCAAGCGTAAAAGCCGATGGGATCAACCAGCAGACCCAGATTCAAGTTTACTTCAGAACAAAGAACAGAAGAGCGAGTCTGGATTGCATAGACAGTTAGCACCTAGTCCATTGCCTGGGATAGGTGAAATGGCATTGCATCTGAAGAGGGTGAGTGGAGAAGATGGAACTTGTTCCAGCAGTGTGCATCGTAACTGTCAGCAAAATGATGGAACAGAGATAAACCTCGACGATCTTCCTCCTGGGTTTGATGCCCCTGCAATTCCATCTTTTGCTTCGTCATCATTTTGTCCTTTGAAGTGTCCTGCTGCTCCAGTGATAGGGCATCCGCAGGAGAAATTTGTTTCTCGCTTACCCATCTCGTACGGAATTCCACTGTCTGTCATGCAGCAATATGGGACACCCCATCTTGCTCCTGGGATACCTTTTCTTCCTTTCCCACCATTACCCTTGGTGCCCCGTCACAAGAAAGGCCCTTCACCTTCTCATCCCATCAATCATGTGGCAGTTAGTCAACCTGCAGAAGGACAACATGACAGGTGTGTTCCTGCAACTTCTCACTCTAATGATAGCAGTCCTAGCACAACTGGTGCCAATCAGGCAGAGTTTAACATTCCATGTGTAAACAACCAATGTGCATCTAAGCGGCCGAGAGAATTCTCGAATGATTTGGGAAGGAGATACTTCAAGCAGCAGAAGTACTGGAATCATAATAATGATAATAATATTAATACAAAATTTGGACCCCCATCTTTCGGGGATAGAAACGGGTGGGGGTGCAACGGAAACAACTCCCAGGGTGGGGCGAACAGCAGCATAGGCGTAGGAAATGTAGCAAATGAGCATACAAGTTCATTTTATGCAGAGGATTTAAGCTATAGGGTTGAGAGAGCTGGAAATAATGCCAGTCAGCATTCACAACACCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001763 GO:0002682 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006464 GO:0006479 GO:0006629 GO:0006720 GO:0006721 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008213 GO:0008276 GO:0008757 GO:0009553 GO:0009555 GO:0009653 GO:0009791 GO:0009893 GO:0009908 GO:0009909 GO:0009910 GO:0009987 GO:0010016 GO:0010223 GO:0010346 GO:0010363 GO:0010452 GO:0010468 GO:0010604 GO:0010638 GO:0010941 GO:0016043 GO:0016108 GO:0016116 GO:0016278 GO:0016279 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018022 GO:0018024 GO:0018193 GO:0018205 GO:0019222 GO:0019538 GO:0022414 GO:0031056 GO:0031058 GO:0031060 GO:0031062 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0032259 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033043 GO:0033044 GO:0034968 GO:0035670 GO:0036211 GO:0040029 GO:0042054 GO:0042800 GO:0043067 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044255 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045088 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048443 GO:0048466 GO:0048467 GO:0048481 GO:0048518 GO:0048519 GO:0048522 GO:0048580 GO:0048581 GO:0048583 GO:0048608 GO:0048646 GO:0048653 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0050776 GO:0050789 GO:0050793 GO:0050794 GO:0051093 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051239 GO:0051241 GO:0051246 GO:0051247 GO:0051276 GO:0051568 GO:0060255 GO:0061458 GO:0065007 GO:0071704 GO:0071840 GO:0080090 GO:0080134 GO:0080135 GO:0090567 GO:0099402 GO:0140096 GO:1901564 GO:1902275 GO:1905269 GO:1905393 GO:2000026 GO:2000241 GO:2000242 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko00310 map00310
Pfam Domains
Protein Families
SET zf-CW

Protein Analysis

2123

Amino Acids

231.56

Weight (kDa)

5.59

Isoelectric Point (pI)

51.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CW PF07496 1066 - 1112 1.3e-11 CW-type Zinc Finger
AWS PF17907 1187 - 1222 7.9e-12 AWS domain
SET PF00856 1236 - 1342 3.9e-25 SET domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 5156
AatII GACGTC 1 cut(s) 3439
Acc16I TGCGCA 1 cut(s) 2714
Acc36I ACCTGC 3 cut(s) 2913, 4417, 5965
Acc65I GGTACC 1 cut(s) 3151
AccB1I GGYRCC 4 cut(s) 1460, 3151, 5893, 6029
AccB7I CCANNNNNTGG 2 cut(s) 4178, 5555
AccI GTMKAC 6 cut(s) 1119, 3232, 3433, 4399, 5226, 5321
AccII CGCG 1 cut(s) 3187
AccIII TCCGGA 1 cut(s) 5016
AciI CCGC 9 cut(s) 647, 1777, 2994, 3185, 3356, 3545, 4827, 5768, 6085
AcoI YGGCCR 2 cut(s) 1768, 6086
AcuI CTGAAG 8 cut(s) 348, 2927, 3534, 4209, 5048, 5346, 5477, 5603
AcyI GRCGYC 2 cut(s) 3436, 5379
AdeI CACNNNGTG 1 cut(s) 3082
AhdI GACNNNNNGTC 1 cut(s) 273
AjiI CACGTC 1 cut(s) 2860
AjnI CCWGG 8 cut(s) 1633, 2428, 2806, 5382, 5554, 5680, 5854, 6234
AjuI GAANNNNNNNTTGG 2 cut(s) 2229, 2261
AloI GAACNNNNNNTCC 2 cut(s) 4247, 4279
Alw21I GWGCWC 2 cut(s) 3204, 3275
Alw44I GTGCAC 1 cut(s) 3271
AlwNI CAGNNNCTG 5 cut(s) 2162, 2186, 2387, 2429, 2934
Aor13HI TCCGGA 1 cut(s) 5016
AoxI GGCC 9 cut(s) 335, 1280, 1302, 1591, 1768, 3712, 5416, 5911, 6086
ApaLI GTGCAC 1 cut(s) 3271
AseI ATTAAT 3 cut(s) 3351, 3477, 6168
Asp700I GAANNNNTTC 3 cut(s) 632, 4860, 5611
Asp718I GGTACC 1 cut(s) 3151
AspLEI GCGC 3 cut(s) 2715, 4900, 5099
AsuII TTCGAA 1 cut(s) 3691
AvaII GGWCC 9 cut(s) 650, 830, 1136, 2925, 3279, 5032, 5087, 5158, 6182
AxyI CCTNAGG 4 cut(s) 1201, 2163, 3379, 3716
BaeGI GKGCMC 3 cut(s) 976, 3275, 5898
BaeI ACNNNNGTAYC 4 cut(s) 4104, 4137, 4815, 4848
BanI GGYRCC 4 cut(s) 1460, 3151, 5893, 6029
BanII GRGCYC 4 cut(s) 8, 103, 1233, 1801
BauI CACGAG 1 cut(s) 4691
BbsI GAAGAC 5 cut(s) 1746, 1852, 4998, 5154, 5218
Bbv12I GWGCWC 2 cut(s) 3204, 3275
BbvCI CCTCAGC 1 cut(s) 2179
BceAI ACGGC 1 cut(s) 3483
BcgI CGANNNNNNTGC 4 cut(s) 2658, 2692, 2721, 2755
BciT130I CCWGG 8 cut(s) 1635, 2430, 2808, 5384, 5556, 5682, 5856, 6236
BciVI GTATCC 1 cut(s) 5853
BclI TGATCA 3 cut(s) 985, 3031, 5305
BfmI CTRYAG 7 cut(s) 357, 858, 1032, 4446, 5331, 5958, 6316
BfuAI ACCTGC 3 cut(s) 2913, 4417, 5965
BfuI GTATCC 1 cut(s) 5853
BglI GCCNNNNNGGC 1 cut(s) 6038
BglII AGATCT 3 cut(s) 12, 1467, 4876
BlpI GCTNAGC 1 cut(s) 2941
BmcAI AGTACT 2 cut(s) 5374, 6140
Bme1390I CCNGG 8 cut(s) 1635, 2430, 2808, 5384, 5556, 5682, 5856, 6236
Bme18I GGWCC 9 cut(s) 650, 830, 1136, 2925, 3279, 5032, 5087, 5158, 6182
BmeRI GACNNNNNGTC 1 cut(s) 273
BmgBI CACGTC 1 cut(s) 2860
BmrFI CCNGG 8 cut(s) 1635, 2430, 2808, 5384, 5556, 5682, 5856, 6236
BpiI GAAGAC 5 cut(s) 1746, 1852, 4998, 5154, 5218
BpmI CTGGAG 9 cut(s) 1138, 1269, 1692, 2418, 2568, 2838, 4473, 5103, 5736
Bpu10I CCTNAGC 2 cut(s) 2179, 2866
Bpu1102I GCTNAGC 1 cut(s) 2941
Bpu14I TTCGAA 1 cut(s) 3691
BpuEI CTTGAG 4 cut(s) 365, 542, 1988, 3746
Bsa29I ATCGAT 2 cut(s) 1380, 4294
BsaBI GATNNNNATC 5 cut(s) 216, 2289, 2358, 3030, 5009
BsaHI GRCGYC 2 cut(s) 3436, 5379
BsaI GGTCTC 2 cut(s) 3627, 5395
BsaWI WCCGGW 2 cut(s) 2982, 5016
BsaXI ACNNNNNCTCC 4 cut(s) 411, 441, 4998, 5028
Bse21I CCTNAGG 4 cut(s) 1201, 2163, 3379, 3716
Bse3DI GCAATG 3 cut(s) 3535, 5549, 5573
Bse8I GATNNNNATC 5 cut(s) 216, 2289, 2358, 3030, 5009
BseAI TCCGGA 1 cut(s) 5016
BseBI CCWGG 8 cut(s) 1635, 2430, 2808, 5384, 5556, 5682, 5856, 6236
BseCI ATCGAT 2 cut(s) 1380, 4294
BseJI GATNNNNATC 5 cut(s) 216, 2289, 2358, 3030, 5009
BseMI GCAATG 3 cut(s) 3535, 5549, 5573
BseRI GAGGAG 5 cut(s) 689, 3630, 4295, 4456, 4751
BseSI GKGCMC 3 cut(s) 976, 3275, 5898
BseX3I CGGCCG 1 cut(s) 6086
BsgI GTGCAG 3 cut(s) 2768, 2865, 5382
Bsh1236I CGCG 1 cut(s) 3187
Bsh1285I CGRYCG 1 cut(s) 6089
BshFI GGCC 9 cut(s) 337, 1282, 1304, 1593, 1770, 3714, 5418, 5913, 6088
BshNI GGYRCC 4 cut(s) 1460, 3151, 5893, 6029
BshVI ATCGAT 2 cut(s) 1380, 4294
BsiEI CGRYCG 1 cut(s) 6089
BsiHKAI GWGCWC 2 cut(s) 3204, 3275
BsiSI CCGG 2 cut(s) 2983, 5017
BsiWI CGTACG 2 cut(s) 2363, 5802
BsmBI CGTCTC 1 cut(s) 414
BsmI GAATGC 4 cut(s) 208, 748, 4965, 6352
BsnI GGCC 9 cut(s) 337, 1282, 1304, 1593, 1770, 3714, 5418, 5913, 6088
Bso31I GGTCTC 2 cut(s) 3627, 5395
Bsp119I TTCGAA 1 cut(s) 3691
Bsp1286I GDGCHC 8 cut(s) 8, 103, 976, 1233, 1801, 3204, 3275, 5898
Bsp13I TCCGGA 1 cut(s) 5016
Bsp1720I GCTNAGC 1 cut(s) 2941
BspACI CCGC 9 cut(s) 647, 1777, 2994, 3185, 3356, 3545, 4827, 5768, 6085
BspANI GGCC 9 cut(s) 337, 1282, 1304, 1593, 1770, 3714, 5418, 5913, 6088
BspDI ATCGAT 2 cut(s) 1380, 4294
BspEI TCCGGA 1 cut(s) 5016
BspFNI CGCG 1 cut(s) 3187
BspHI TCATGA 3 cut(s) 1537, 1684, 3843
BspMAI CTGCAG 2 cut(s) 361, 5962
BspMI ACCTGC 3 cut(s) 2913, 4417, 5965
BspQI GCTCTTC 1 cut(s) 5504
BspT104I TTCGAA 1 cut(s) 3691
BspT107I GGYRCC 4 cut(s) 1460, 3151, 5893, 6029
BspTNI GGTCTC 2 cut(s) 3627, 5395
BsrDI GCAATG 3 cut(s) 3535, 5549, 5573
BssNAI GTATAC 2 cut(s) 3233, 4400
BssNI GRCGYC 2 cut(s) 3436, 5379
BssSI CACGAG 1 cut(s) 4691
BssT1I CCWWGG 5 cut(s) 933, 1017, 1802, 2916, 5889
Bst1107I GTATAC 2 cut(s) 3233, 4400
Bst2BI CACGAG 1 cut(s) 4691
Bst2UI CCWGG 8 cut(s) 1635, 2430, 2808, 5384, 5556, 5682, 5856, 6236
Bst6I CTCTTC 8 cut(s) 495, 618, 1874, 1947, 3999, 5398, 5504, 5579
BstACI GRCGYC 2 cut(s) 3436, 5379
BstBI TTCGAA 1 cut(s) 3691
BstC8I GCNNGC 7 cut(s) 604, 2641, 2681, 2947, 3466, 4215, 5444
BstENI CCTNNNNNAGG 3 cut(s) 915, 5559, 5961
BstFNI CGCG 1 cut(s) 3187
BstHHI GCGC 3 cut(s) 2715, 4900, 5099
BstMCI CGRYCG 1 cut(s) 6089
BstMWI GCNNNNNNNGC 8 cut(s) 1776, 2946, 3245, 3794, 4750, 6018, 6038, 6258
BstNI CCWGG 8 cut(s) 1635, 2430, 2808, 5384, 5556, 5682, 5856, 6236
BstNSI RCATGY 4 cut(s) 321, 771, 897, 3879
BstSCI CCNGG 8 cut(s) 1633, 2428, 2806, 5382, 5554, 5680, 5854, 6234
BstSFI CTRYAG 7 cut(s) 357, 858, 1032, 4446, 5331, 5958, 6316
BstSLI GKGCMC 3 cut(s) 976, 3275, 5898
BstUI CGCG 1 cut(s) 3187
BstV2I GAAGAC 5 cut(s) 1746, 1852, 4998, 5154, 5218
BstX2I RGATCY 6 cut(s) 12, 217, 353, 1467, 2425, 4876
BstXI CCANNNNNNTGG 2 cut(s) 214, 1355
BstYI RGATCY 6 cut(s) 12, 217, 353, 1467, 2425, 4876
BstZ17I GTATAC 2 cut(s) 3233, 4400
BstZI CGGCCG 1 cut(s) 6086
Bsu15I ATCGAT 2 cut(s) 1380, 4294
Bsu36I CCTNAGG 4 cut(s) 1201, 2163, 3379, 3716
BsuI GTATCC 1 cut(s) 5853
BsuRI GGCC 9 cut(s) 337, 1282, 1304, 1593, 1770, 3714, 5418, 5913, 6088
BsuTUI ATCGAT 2 cut(s) 1380, 4294
BtrI CACGTC 1 cut(s) 2860
BtsI GCAGTG 4 cut(s) 2780, 3865, 4855, 5626
BveI ACCTGC 3 cut(s) 2913, 4417, 5965
Cac8I GCNNGC 7 cut(s) 604, 2641, 2681, 2947, 3466, 4215, 5444
CaiI CAGNNNCTG 5 cut(s) 2162, 2186, 2387, 2429, 2934
CciI TCATGA 3 cut(s) 1537, 1684, 3843
CfoI GCGC 3 cut(s) 2715, 4900, 5099
ClaI ATCGAT 2 cut(s) 1380, 4294
CseI GACGC 3 cut(s) 1210, 2076, 5368
DraIII CACNNNGTG 1 cut(s) 3082
DrdI GACNNNNNNGTC 1 cut(s) 5156
DriI GACNNNNNGTC 1 cut(s) 273
DseDI GACNNNNNNGTC 1 cut(s) 5156
EaeI YGGCCR 2 cut(s) 1768, 6086
EagI CGGCCG 1 cut(s) 6086
Eam1104I CTCTTC 8 cut(s) 495, 618, 1874, 1947, 3999, 5398, 5504, 5579
Eam1105I GACNNNNNGTC 1 cut(s) 273
EarI CTCTTC 8 cut(s) 495, 618, 1874, 1947, 3999, 5398, 5504, 5579
EclXI CGGCCG 1 cut(s) 6086
Eco130I CCWWGG 5 cut(s) 933, 1017, 1802, 2916, 5889
Eco24I GRGCYC 4 cut(s) 8, 103, 1233, 1801
Eco31I GGTCTC 2 cut(s) 3627, 5395
Eco47I GGWCC 9 cut(s) 650, 830, 1136, 2925, 3279, 5032, 5087, 5158, 6182
Eco52I CGGCCG 1 cut(s) 6086
Eco57I CTGAAG 8 cut(s) 348, 2927, 3534, 4209, 5048, 5346, 5477, 5603
Eco81I CCTNAGG 4 cut(s) 1201, 2163, 3379, 3716
EcoNI CCTNNNNNAGG 3 cut(s) 915, 5559, 5961
EcoO109I RGGNCCY 6 cut(s) 650, 1136, 1302, 5032, 5087, 5912
EcoRI GAATTC 3 cut(s) 2332, 5808, 6095
EcoRII CCWGG 8 cut(s) 1633, 2428, 2806, 5382, 5554, 5680, 5854, 6234
EcoT14I CCWWGG 5 cut(s) 933, 1017, 1802, 2916, 5889
EcoT22I ATGCAT 3 cut(s) 750, 3397, 3877
EcoT38I GRGCYC 4 cut(s) 8, 103, 1233, 1801
ErhI CCWWGG 5 cut(s) 933, 1017, 1802, 2916, 5889
Esp3I CGTCTC 1 cut(s) 414
FauI CCCGC 1 cut(s) 640
FauNDI CATATG 3 cut(s) 398, 670, 948
FbaI TGATCA 3 cut(s) 985, 3031, 5305
FblI GTMKAC 6 cut(s) 1119, 3232, 3433, 4399, 5226, 5321
FriOI GRGCYC 4 cut(s) 8, 103, 1233, 1801
FspAI RTGCGCAY 1 cut(s) 2714
FspI TGCGCA 1 cut(s) 2714
GlaI GCGC 3 cut(s) 2714, 4899, 5098
GsuI CTGGAG 9 cut(s) 1138, 1269, 1692, 2418, 2568, 2838, 4473, 5103, 5736
HaeIII GGCC 9 cut(s) 337, 1282, 1304, 1593, 1770, 3714, 5418, 5913, 6088
HapII CCGG 2 cut(s) 2983, 5017
HgaI GACGC 3 cut(s) 1210, 2076, 5368
HhaI GCGC 3 cut(s) 2715, 4900, 5099
Hin1I GRCGYC 2 cut(s) 3436, 5379
Hin6I GCGC 3 cut(s) 2713, 4898, 5097
HinP1I GCGC 3 cut(s) 2713, 4898, 5097
HincII GTYRAC 5 cut(s) 76, 2091, 3160, 3434, 5954
HindII GTYRAC 5 cut(s) 76, 2091, 3160, 3434, 5954
HindIII AAGCTT 5 cut(s) 600, 2641, 4742, 4811, 5021
HpaI GTTAAC 1 cut(s) 3160
HpaII CCGG 2 cut(s) 2983, 5017
Hpy99I CGWCG 3 cut(s) 3438, 4769, 5672
HpyCH4IV ACGT 3 cut(s) 2859, 3436, 4208
HpyF10VI GCNNNNNNNGC 8 cut(s) 1776, 2946, 3245, 3794, 4750, 6018, 6038, 6258
HpySE526I ACGT 3 cut(s) 2859, 3436, 4208
Hsp92I GRCGYC 2 cut(s) 3436, 5379
HspAI GCGC 3 cut(s) 2713, 4898, 5097
KflI GGGWCCC 1 cut(s) 650
Kpn2I TCCGGA 1 cut(s) 5016
KpnI GGTACC 1 cut(s) 3155
Ksp22I TGATCA 3 cut(s) 985, 3031, 5305
KspAI GTTAAC 1 cut(s) 3160
LguI GCTCTTC 1 cut(s) 5504
LmnI GCTCC 8 cut(s) 52, 1804, 2278, 3617, 4043, 4156, 5755, 5857
MaeII ACGT 3 cut(s) 2859, 3436, 4208
MfeI CAATTG 2 cut(s) 784, 2224
MflI RGATCY 6 cut(s) 12, 217, 353, 1467, 2425, 4876
MhlI GDGCHC 8 cut(s) 8, 103, 976, 1233, 1801, 3204, 3275, 5898
MlyI GAGTC 6 cut(s) 167, 283, 1996, 4292, 5129, 5525
MmeI TCCRAC 8 cut(s) 454, 1641, 3342, 3464, 3745, 4546, 4737, 5295
Mph1103I ATGCAT 3 cut(s) 750, 3397, 3877
MroI TCCGGA 1 cut(s) 5016
MroXI GAANNNNTTC 3 cut(s) 632, 4860, 5611
MspA1I CMGCKG 4 cut(s) 1158, 2339, 3239, 4829
MspI CCGG 2 cut(s) 2983, 5017
MspR9I CCNGG 8 cut(s) 1635, 2430, 2808, 5384, 5556, 5682, 5856, 6236
MunI CAATTG 2 cut(s) 784, 2224
Mva1269I GAATGC 4 cut(s) 208, 748, 4965, 6352
MvaI CCWGG 8 cut(s) 1635, 2430, 2808, 5384, 5556, 5682, 5856, 6236
MvnI CGCG 1 cut(s) 3187
MwoI GCNNNNNNNGC 8 cut(s) 1776, 2946, 3245, 3794, 4750, 6018, 6038, 6258
NdeI CATATG 3 cut(s) 398, 670, 948
NmeAIII GCCGAG 1 cut(s) 6114
NsbI TGCGCA 1 cut(s) 2714
NsiI ATGCAT 3 cut(s) 750, 3397, 3877
NspI RCATGY 4 cut(s) 321, 771, 897, 3879
NspV TTCGAA 1 cut(s) 3691
PagI TCATGA 3 cut(s) 1537, 1684, 3843
PasI CCCWGGG 1 cut(s) 6235
PciSI GCTCTTC 1 cut(s) 5504
PcsI WCGNNNNNNNCGW 1 cut(s) 5179
PctI GAATGC 4 cut(s) 208, 748, 4965, 6352
PdmI GAANNNNTTC 3 cut(s) 632, 4860, 5611
Pfl23II CGTACG 2 cut(s) 2363, 5802
PflFI GACNNNGTC 1 cut(s) 425
PflMI CCANNNNNTGG 2 cut(s) 4178, 5555
PfoI TCCNGGA 2 cut(s) 2428, 5382
PleI GAGTC 6 cut(s) 166, 282, 1995, 4291, 5128, 5524
PpsI GAGTC 6 cut(s) 166, 282, 1995, 4291, 5128, 5524
PpuMI RGGWCCY 4 cut(s) 650, 1136, 5032, 5087
PshBI ATTAAT 3 cut(s) 3351, 3477, 6168
Psp5II RGGWCCY 4 cut(s) 650, 1136, 5032, 5087
Psp6I CCWGG 8 cut(s) 1633, 2428, 2806, 5382, 5554, 5680, 5854, 6234
PspGI CCWGG 8 cut(s) 1633, 2428, 2806, 5382, 5554, 5680, 5854, 6234
PspLI CGTACG 2 cut(s) 2363, 5802
PspPPI RGGWCCY 4 cut(s) 650, 1136, 5032, 5087
PstI CTGCAG 2 cut(s) 361, 5962
PstNI CAGNNNCTG 5 cut(s) 2162, 2186, 2387, 2429, 2934
PsuI RGATCY 6 cut(s) 12, 217, 353, 1467, 2425, 4876
PsyI GACNNNGTC 1 cut(s) 425
PvuII CAGCTG 3 cut(s) 1158, 2339, 3239
SalI GTCGAC 1 cut(s) 3432
SapI GCTCTTC 1 cut(s) 5504
ScaI AGTACT 2 cut(s) 5374, 6140
SchI GAGTC 6 cut(s) 167, 283, 1996, 4292, 5129, 5525
ScrFI CCNGG 8 cut(s) 1635, 2430, 2808, 5384, 5556, 5682, 5856, 6236
SduI GDGCHC 8 cut(s) 8, 103, 976, 1233, 1801, 3204, 3275, 5898
SfcI CTRYAG 7 cut(s) 357, 858, 1032, 4446, 5331, 5958, 6316
SfuI TTCGAA 1 cut(s) 3691
SinI GGWCC 9 cut(s) 650, 830, 1136, 2925, 3279, 5032, 5087, 5158, 6182
SmlI CTYRAG 4 cut(s) 344, 521, 1967, 3725
SmoI CTYRAG 4 cut(s) 344, 521, 1967, 3725
SsiI CCGC 9 cut(s) 647, 1777, 2994, 3185, 3356, 3545, 4827, 5768, 6085
SspI AATATT 6 cut(s) 906, 1534, 1642, 3514, 3601, 6166
StyD4I CCNGG 8 cut(s) 1633, 2428, 2806, 5382, 5554, 5680, 5854, 6234
StyI CCWWGG 5 cut(s) 933, 1017, 1802, 2916, 5889
TaiI ACGT 3 cut(s) 2862, 3439, 4211
TatI WGTACW 3 cut(s) 3414, 5372, 6138
TauI GCSGC 2 cut(s) 1779, 6088
Tth111I GACNNNGTC 1 cut(s) 425
Van91I CCANNNNNTGG 2 cut(s) 4178, 5555
VneI GTGCAC 1 cut(s) 3271
VpaK11BI GGWCC 9 cut(s) 650, 830, 1136, 2925, 3279, 5032, 5087, 5158, 6182
VspI ATTAAT 3 cut(s) 3351, 3477, 6168
XagI CCTNNNNNAGG 3 cut(s) 915, 5559, 5961
XceI RCATGY 4 cut(s) 321, 771, 897, 3879
XcmI CCANNNNNNNNNTGG 3 cut(s) 5350, 5852, 5938
XmiI GTMKAC 6 cut(s) 1119, 3232, 3433, 4399, 5226, 5321
XmnI GAANNNNTTC 3 cut(s) 632, 4860, 5611
ZraI GACGTC 1 cut(s) 3437
ZrmI AGTACT 2 cut(s) 5374, 6140
Zsp2I ATGCAT 3 cut(s) 750, 3397, 3877
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.