Prupe.1G489300_v2.0.a1

Histone-lysine N-methyltransferase ASHH2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0007450
Physical Location & Seq
Reverse (-)
40735339 .. 40743395
8057 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G489300.1

Sequence Viewer

Length: 3993 bp
ATGGCTTCTTGTGAAAATTCGGCTTTTGTTGACGGTCTTTTGTGTGAGTCTGTTACAGAGCAGCAGATGGGCTCAGAGATTTTGGTGCAATCTATTTCTGAGCTGCCCTCCTGCTCGGACGATAAGCCTTTACTTGATTCTAATGTGGACCCTACCAGTATATCAGGTAGGTCTCTGGGATTGTCTAGGAATGACAATGCTGATGCTGGTTGTATGAGCTGGGGTGAGATTGTAGATGTTGATATAGATGGTTTGATGGGTGAGCATGAAAATGCTAATGTTTCGGAACCGGGAAATATTTTGACCAGGGAATGGGGGCTGAACAGATGTTGCTTGACTGAGAGTCAGAGTAATGTTGATGACTCAAATAGGGAAGGTGGGCCCCAAGGTGAGGATAGATCTGCTGGTTCATTTGGGGACTGTGAAATCCCTTTGGAAGTCATACCTCAGACTGTTTCACCAGGACATGGTATCCAACAGGATGAGCAAAGGTATGATAAGAGTGTTAGTTATCTGTTTTCTGAAGGGGTTAGGGAAGTTACGGAAGAGATGAAGAATGATATCCATGAACTAGTCTCTCCTTTACAGGGTTGTGAAATTCCTTCAGAATTAGCTGACATGGAAGCTTGTGGAAGCTCCCAACAAGAAGAGAAGGGTTTCAACATCATATCTGGTTCCTCTTTTGAAGCTGCTTGTGAGGAAAGTGTTGTTTTGGCCAGGATAGAAGCAGATTTGTGCAACAGGACATCACCTAAACGGGATGGTGGAATGCCTTCAGAAGTATTATATGCATGTGATTTAGTAAGTAATTGTGTCTGGAAGAATGAAGAGATGGAGGACAATGGTTTTAGTGGTCTGAATAGCCTCTCTACAGAAAGGACTACAGAGGTTGTTGAGACGAGGTGCAACCAAATCTTGCCTTCACAAGGTTTCCGGAGGACCTTGGAAGATTTACATATGCCTGATTCACCCAGTAGTTGCGCTCAACAGAATGATCTGAGGAGTGATAAGGTTAATGGCGGTTCCTTTGTTGAAAGGGCTACAGAATATTTGGAAGAGACAAGTGATGCTCTGCCAGATGAAAGGGCTGTAATTCACACCCAGATATTACCTGTAAAAGTTAATGTTTACTATTCTAAGGAAGGTTCTTCCAAAGTAGCCCCTGACTGCGTTATTGAGAACTCTGTTTCCATGCAGTCATGTCAAACCTTTGGGACTGCCAATAACAGTGTCAATGGGCCCTCTGCTGATAGTGTTACAGAGGTTGTTGAGATGGAAAGCCATATTGATGTGCACAATCAAATAGTGCCTCCAGAGTGTTGCCAGAGGGCCTTTGAAGGTTCGCATGTGTCAGATTCACCGAGTGTTTGCACCCAAGAGAATGGTGAGAAGAGTAATGATTTTCTCTCTGCCAAAAGGGTAACAGAGTTTGAAGAACAGAAAAGTGATGCTACAACCGACATAAAGGTTGAAATTGGCGCCCAGATATTACTACTGGAGGAAAAAGCCTCCAATCTGAAGGAAGTTTCTTCTGAACTAGCCCCCAAAAGTATTCATGAGAAATCTGTTTCTATGCAATCGTCTCAACCTTTTGATATTGTCAACAGTGGTTCTTCTGAGAGGCTGGATGTACCAGATAAGGATTCTCCTGCTCATGTTGATTCTAGCACTTCATTTGACCGTTATGGAGAGATGGATCATGAAGGAAATGATAATGTCAGAGTTGATTGTGTTTCTAACACCAAATGTGTTGCATTGTCTTCTCGAAGGAGCGGCCGAAGCCGCAAGACTCAGACAAAAAGGGCTCCAAGGAAAGGCAGGAACACATCTAAAGTGTTAGACCCGTTTGGAAGTGTTGAAATTGTCTTCAAGGCTGCTGGAAGGAAGAGAAGCTGCCTGTCCAAACCAGCTCGTTCTTCTATCTGGGGATTATTGGGGAATGTTACACAATCTTTTGAAGAGAGTAACAGGCTTGAGGTCAGTCAAGGTCTGATTCAAGGATCCCAGAAAGGAAGGGGTGGCCAAAGAAGTGGAAAACGGAATCCAAGTGGGGCTAGTGGAAACTCACGAGGGTCAAGGGGGAAATGCCGTGCTTCAACTAATCGTGTTCGTTTGAAGGTTAAATTGGGGAAAGAAGTGGGTAAAAGTTCTTTCTATATCACGGTACCTGAGGTTGTTGATAACACAGCATATGAAAATTCTGTTGAAAAAGAGAATGGCATTGAGGGTAATTGGAATAAAGAAGCCACTCTTAGGGAGGATAAAACTTGTCCAGATGCTCCTGTTCTGGATGGAGATCTGGCTAATAAGGACTTGGAGAGTGTTGTTCTTACAGAGAATTCAGCTGAAGATGTGATAGAGAATTTTCCTGGGGGTTCATCTCATACAATTGCTGTATCATCAGGAGGATCTGTTGGGACTAACTATAGAGATCCTGGAACTTCACCTGATTCAGAAGTAACCAATTTAGTTCCGGATGCAGATGTTGAGGCAAGACCACTGGAAGATTCTAATGGTATTGTTTTGACTTCTGACAAGGCTTTTTCTGCTTCTGGAGACTTTATTGGCACTAAGAGAGGAAAGAAGAAACATAAAGTACCTCATGCGGAAAATTGTGTTCGGGAAGATGGTATACCGTGTCCCGCAAGCATAAATAAAGAAAAACCATCAAAACAAGATGGACGCAGACAGAATGTTAGTCAGGATTTTTGCCCCAGTGAGACGTTCACTTCATCCACCTGTGCAAATGCTTCAAGCAATTCATCAAGCGACATGGAATCATCTTTGGAGCCCTTGCGTTTGTCAGGAGAAACTGACCATGGAATTTCTAGAGATGTTCTCAAAGTTGAAATTGGTGCAGAAGCTAAAACACATTGCAATCTAGATGTAGGCTTAGGGTTATCAAAATCACAATCCTCAAAAACTAAGGGACTGAAGCCTCCGAAGGGCAGGTCCAGAGGCTGTGGCTCAGCGAGCAAGAAAGGTAATTCTCATAGAGTGAGGGAAAACCAAAAGAAGTCTGTTAATCAGAAGAATGCTATGGAGAAGGCTGTTGGTGATCAGGTTGCATGCAAAGTGGAAAGTCTTCCAGAATCAGATGATCACTTAGTGGATGGAATTAGAAAAGCCAACTCTGTCAAGGATGCTGTATGCATAGGTGTTCCTAACTTGGACACTGTACCTGTTGACTTAGATAAGCAGTATGTACCGCCACGTAATGCTTGGGTGCTCTGTGATGATTGTCATAAATGGAGGCGTATACCAGCTGAGCTTGCAGATGTTATAGATGAAATAAAGTGCACATGGACCTGTAGAGATAATAAGGATAAAGCCTTTGCTGATTGTTCAATCCCTCAAGAGAAGTCAAATTCAGAGATTAATGCAGAGTTGGATATATCAGATGCCTCAGGTGACGAAGATGCTTCCGTCACCCGATTAAATTATAAAGAATTGGAACGTCGGCGCCCAACAGTTTCCCAACAGAATGTTGCAAGCATCAAAACTAATCAGTTCCTTCATCGTAACCGTAAAACTCAGACTATTGACGAGATAATGGTTTGTCATTGCAAACCACCCTCAGATGGCCAGTTGGGTTGTGGAGATGATTGTCTTAATCGAATGCTTAACATTGAGTGTATTCGAGGAGCCTGTCCTTGTAGAGACCTTTGTTCAAATCAACAGTTCCAAAAACGCCGATATGCCAAACTGGAGAAGTTTCGATGTGGGAAGAAGGGTTATGGGCTCAGGTTGCTTGATGATATATTTAAAGGGCAATTTCTCATTGAATATGTTGGAGAGGTGCTTGATACACATGCTTATGAGGCACGGCAAAAAGAGTATGCTTTAAAGGCTCACAGACATTTCTACTTCATGACATTGAATGGCAGTGAGGTAATTGATGCATGTGCAAAGGGAAATTTGGGGCGTTTCATTAACCATAGTTGTGATCCTAATTGTCGCACAGAAAAGATTTCCCTCTCCCTGCCTCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001763 GO:0002682 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006464 GO:0006479 GO:0006629 GO:0006720 GO:0006721 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008213 GO:0008276 GO:0008757 GO:0009553 GO:0009555 GO:0009653 GO:0009791 GO:0009893 GO:0009908 GO:0009909 GO:0009910 GO:0009987 GO:0010016 GO:0010223 GO:0010346 GO:0010363 GO:0010452 GO:0010468 GO:0010604 GO:0010638 GO:0010941 GO:0016043 GO:0016108 GO:0016116 GO:0016278 GO:0016279 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018022 GO:0018024 GO:0018193 GO:0018205 GO:0019222 GO:0019538 GO:0022414 GO:0031056 GO:0031058 GO:0031060 GO:0031062 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0032259 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033043 GO:0033044 GO:0034968 GO:0035670 GO:0036211 GO:0040029 GO:0042054 GO:0042800 GO:0043067 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044255 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045088 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048443 GO:0048466 GO:0048467 GO:0048481 GO:0048518 GO:0048519 GO:0048522 GO:0048580 GO:0048581 GO:0048583 GO:0048608 GO:0048646 GO:0048653 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0050776 GO:0050789 GO:0050793 GO:0050794 GO:0051093 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051239 GO:0051241 GO:0051246 GO:0051247 GO:0051276 GO:0051568 GO:0060255 GO:0061458 GO:0065007 GO:0071704 GO:0071840 GO:0080090 GO:0080134 GO:0080135 GO:0090567 GO:0099402 GO:0140096 GO:1901564 GO:1902275 GO:1905269 GO:1905393 GO:2000026 GO:2000241 GO:2000242 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko00310 map00310
Pfam Domains
Protein Families
SET zf-CW

Protein Analysis

1331

Amino Acids

145.35

Weight (kDa)

5.14

Isoelectric Point (pI)

50.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3444
Acc36I ACCTGC 1 cut(s) 2940
Acc65I GGTACC 1 cut(s) 2164
AccB1I GGYRCC 3 cut(s) 1478, 2164, 3462
AccB7I CCANNNNNTGG 2 cut(s) 312, 467
AccBSI CCGCTC 1 cut(s) 1773
AccI GTMKAC 2 cut(s) 2632, 3259
AccIII TCCGGA 2 cut(s) 933, 2473
AciI CCGC 6 cut(s) 1020, 1773, 1783, 2606, 2643, 3209
AclWI GGATC 6 cut(s) 1704, 1995, 2008, 2416, 2426, 3941
AcoI YGGCCR 4 cut(s) 714, 1774, 2020, 3583
AcsI RAATTY 8 cut(s) 16, 597, 2197, 2338, 2362, 2823, 3367, 3916
AcuI CTGAAG 6 cut(s) 543, 588, 759, 1538, 2367, 2954
AcyI GRCGYC 2 cut(s) 1479, 3463
AdeI CACNNNGTG 2 cut(s) 1364, 3109
AfaI GTAC 5 cut(s) 1632, 2166, 2598, 3180, 3207
AfiI CCNNNNNNNGG 9 cut(s) 312, 391, 467, 587, 926, 1814, 2253, 2945, 3581
AhdI GACNNNNNGTC 1 cut(s) 342
AhlI ACTAGT 1 cut(s) 571
AjnI CCWGG 5 cut(s) 305, 460, 716, 2368, 2434
AjuI GAANNNNNNNTTGG 2 cut(s) 2108, 2140
AloI GAACNNNNNNTCC 2 cut(s) 1172, 1204
Alw21I GWGCWC 3 cut(s) 1296, 3231, 3302
Alw26I GTCTC 8 cut(s) 177, 580, 890, 1052, 1587, 2550, 2714, 3654
Alw44I GTGCAC 2 cut(s) 1292, 3298
AlwI GGATC 6 cut(s) 1704, 1995, 2008, 2416, 2426, 3941
AlwNI CAGNNNCTG 1 cut(s) 2961
Aor13HI TCCGGA 2 cut(s) 933, 2473
AoxI GGCC 7 cut(s) 380, 714, 1238, 1329, 1774, 2020, 3583
ApaI GGGCCC 2 cut(s) 384, 1242
ApaLI GTGCAC 2 cut(s) 1292, 3298
ApeKI GCWGC 5 cut(s) 61, 103, 689, 1874, 1893
ApoI RAATTY 8 cut(s) 16, 597, 2197, 2338, 2362, 2823, 3367, 3916
ArsI GACNNNNNNTTYG 2 cut(s) 2936, 2968
AseI ATTAAT 1 cut(s) 3378
Asp700I GAANNNNTTC 3 cut(s) 656, 772, 3084
Asp718I GGTACC 1 cut(s) 2164
AspLEI GCGC 3 cut(s) 983, 1481, 3465
AspS9I GGNCC 9 cut(s) 148, 380, 381, 939, 1238, 1239, 1329, 2952, 3306
AsuC2I CCSGG 1 cut(s) 291
AvaII GGWCC 4 cut(s) 148, 939, 2952, 3306
AxyI CCTNAGG 2 cut(s) 2169, 3406
BaeGI GKGCMC 4 cut(s) 384, 1242, 1296, 3302
BalI TGGCCA 3 cut(s) 716, 2022, 3585
BamHI GGATCC 1 cut(s) 2000
BanI GGYRCC 3 cut(s) 1478, 2164, 3462
BanII GRGCYC 6 cut(s) 74, 384, 1242, 1807, 2793, 3744
BarI GAAGNNNNNNTAC 2 cut(s) 2616, 2648
BauI CACGAG 1 cut(s) 2067
BbsI GAAGAC 3 cut(s) 1752, 1858, 3077
Bbv12I GWGCWC 3 cut(s) 1296, 3231, 3302
BbvI GCAGC 5 cut(s) 73, 90, 676, 1861, 1880
BceAI ACGGC 2 cut(s) 2073, 3842
BciT130I CCWGG 5 cut(s) 307, 462, 718, 2370, 2436
BciVI GTATCC 1 cut(s) 482
BclI TGATCA 2 cut(s) 3058, 3100
BcnI CCSGG 1 cut(s) 291
BcoDI GTCTC 8 cut(s) 177, 580, 890, 1052, 1587, 2550, 2714, 3654
BcuI ACTAGT 1 cut(s) 571
BfaI CTAG 7 cut(s) 186, 572, 1538, 1665, 2055, 2829, 2882
BfmI CTRYAG 5 cut(s) 870, 882, 1041, 2425, 3310
BfoI RGCGCY 2 cut(s) 1482, 3466
BfuAI ACCTGC 1 cut(s) 2940
BfuI GTATCC 1 cut(s) 482
BglII AGATCT 2 cut(s) 398, 2296
BisI GCNGC 7 cut(s) 62, 104, 690, 1774, 1783, 1875, 1894
BlpI GCTNAGC 2 cut(s) 2968, 3267
BlsI GCNGC 7 cut(s) 63, 105, 691, 1775, 1784, 1876, 1895
Bme1390I CCNGG 6 cut(s) 291, 307, 462, 718, 2370, 2436
Bme18I GGWCC 4 cut(s) 148, 939, 2952, 3306
BmeRI GACNNNNNGTC 1 cut(s) 342
BmgT120I GGNCC 9 cut(s) 148, 380, 381, 939, 1238, 1239, 1329, 2952, 3306
BmrFI CCNGG 6 cut(s) 291, 307, 462, 718, 2370, 2436
BmrI ACTGGG 2 cut(s) 966, 2709
BmuI ACTGGG 2 cut(s) 966, 2709
BpiI GAAGAC 3 cut(s) 1752, 1858, 3077
BplI GAGNNNNNCTC 4 cut(s) 92, 124, 2823, 2855
BpmI CTGGAG 4 cut(s) 1296, 1517, 2574, 3728
Bpu10I CCTNAGC 2 cut(s) 2893, 3743
Bpu1102I GCTNAGC 2 cut(s) 2968, 3267
BpuEI CTTGAG 2 cut(s) 1994, 3339
BpuMI CCSGG 1 cut(s) 291
BsaAI YACGTR 1 cut(s) 3215
BsaBI GATNNNNATC 1 cut(s) 2295
BsaHI GRCGYC 2 cut(s) 1479, 3463
BsaI GGTCTC 2 cut(s) 177, 3654
BsaJI CCNNGG 6 cut(s) 306, 385, 942, 1808, 2369, 2818
BsaWI WCCGGW 2 cut(s) 933, 2473
BsaXI ACNNNNNCTCC 2 cut(s) 2397, 2427
Bsc4I CCNNNNNNNGG 9 cut(s) 312, 391, 467, 587, 926, 1814, 2253, 2945, 3581
Bse1I ACTGG 7 cut(s) 156, 972, 1500, 2505, 2715, 3586, 3711
Bse21I CCTNAGG 2 cut(s) 2169, 3406
Bse3DI GCAATG 2 cut(s) 2872, 3562
Bse8I GATNNNNATC 1 cut(s) 2295
BseAI TCCGGA 2 cut(s) 933, 2473
BseBI CCWGG 5 cut(s) 307, 462, 718, 2370, 2436
BseDI CCNNGG 6 cut(s) 306, 385, 942, 1808, 2369, 2818
BseGI GGATG 8 cut(s) 487, 766, 1633, 2296, 2482, 2732, 3118, 3148
BseJI GATNNNNATC 1 cut(s) 2295
BseLI CCNNNNNNNGG 9 cut(s) 312, 391, 467, 587, 926, 1814, 2253, 2945, 3581
BseMI GCAATG 2 cut(s) 2872, 3562
BseNI ACTGG 7 cut(s) 156, 972, 1500, 2505, 2715, 3586, 3711
BseRI GAGGAG 2 cut(s) 1015, 3657
BseSI GKGCMC 4 cut(s) 384, 1242, 1296, 3302
BseX3I CGGCCG 1 cut(s) 1774
BseXI GCAGC 5 cut(s) 73, 90, 676, 1861, 1880
BseYI CCCAGC 1 cut(s) 219
BsgI GTGCAG 1 cut(s) 2877
Bsh1285I CGRYCG 1 cut(s) 1777
BshFI GGCC 7 cut(s) 382, 716, 1240, 1331, 1776, 2022, 3585
BshNI GGYRCC 3 cut(s) 1478, 2164, 3462
BsiEI CGRYCG 1 cut(s) 1777
BsiHKAI GWGCWC 3 cut(s) 1296, 3231, 3302
BsiSI CCGG 3 cut(s) 290, 934, 2474
BslFI GGGAC 5 cut(s) 431, 1228, 2431, 2625, 2943
BslI CCNNNNNNNGG 9 cut(s) 312, 391, 467, 587, 926, 1814, 2253, 2945, 3581
BsmAI GTCTC 8 cut(s) 177, 580, 890, 1052, 1587, 2550, 2714, 3654
BsmBI CGTCTC 3 cut(s) 890, 1587, 2714
BsmFI GGGAC 5 cut(s) 431, 1228, 2431, 2625, 2943
BsmI GAATGC 3 cut(s) 774, 3040, 3624
BsnI GGCC 7 cut(s) 382, 716, 1240, 1331, 1776, 2022, 3585
Bso31I GGTCTC 2 cut(s) 177, 3654
Bsp120I GGGCCC 2 cut(s) 380, 1238
Bsp1286I GDGCHC 9 cut(s) 74, 384, 1242, 1296, 1807, 2793, 3231, 3302, 3744
Bsp13I TCCGGA 2 cut(s) 933, 2473
Bsp1720I GCTNAGC 2 cut(s) 2968, 3267
Bsp19I CCATGG 1 cut(s) 2818
BspACI CCGC 6 cut(s) 1020, 1773, 1783, 2606, 2643, 3209
BspANI GGCC 7 cut(s) 382, 716, 1240, 1331, 1776, 2022, 3585
BspEI TCCGGA 2 cut(s) 933, 2473
BspHI TCATGA 3 cut(s) 1555, 1699, 3870
BspMI ACCTGC 1 cut(s) 2940
BspPI GGATC 6 cut(s) 1704, 1995, 2008, 2416, 2426, 3941
BspT107I GGYRCC 3 cut(s) 1478, 2164, 3462
BspTNI GGTCTC 2 cut(s) 177, 3654
BsrBI CCGCTC 1 cut(s) 1773
BsrDI GCAATG 2 cut(s) 2872, 3562
BsrI ACTGG 7 cut(s) 156, 972, 1500, 2505, 2715, 3586, 3711
BssECI CCNNGG 6 cut(s) 306, 385, 942, 1808, 2369, 2818
BssNAI GTATAC 2 cut(s) 2633, 3260
BssNI GRCGYC 2 cut(s) 1479, 3463
BssSI CACGAG 1 cut(s) 2067
BssT1I CCWWGG 4 cut(s) 385, 942, 1808, 2818
Bst1107I GTATAC 2 cut(s) 2633, 3260
Bst2BI CACGAG 1 cut(s) 2067
Bst2UI CCWGG 5 cut(s) 307, 462, 718, 2370, 2436
Bst6I CTCTTC 7 cut(s) 540, 642, 822, 1050, 1385, 1880, 1953
BstACI GRCGYC 2 cut(s) 1479, 3463
BstBAI YACGTR 1 cut(s) 3215
BstC8I GCNNGC 5 cut(s) 2647, 2974, 3070, 3273, 3493
BstDSI CCRYGG 1 cut(s) 2818
BstENI CCTNNNNNAGG 1 cut(s) 924
BstF5I GGATG 8 cut(s) 487, 766, 1633, 2296, 2482, 2732, 3118, 3148
BstH2I RGCGCY 2 cut(s) 1482, 3466
BstHHI GCGC 3 cut(s) 983, 1481, 3465
BstMAI GTCTC 8 cut(s) 177, 580, 890, 1052, 1587, 2550, 2714, 3654
BstMCI CGRYCG 1 cut(s) 1777
BstMWI GCNNNNNNNGC 8 cut(s) 1779, 1782, 2546, 2973, 3272, 3748, 3821, 3848
BstNI CCWGG 5 cut(s) 307, 462, 718, 2370, 2436
BstNSI RCATGY 5 cut(s) 795, 1349, 3072, 3815, 3906
BstSCI CCNGG 6 cut(s) 289, 305, 460, 716, 2368, 2434
BstSFI CTRYAG 5 cut(s) 870, 882, 1041, 2425, 3310
BstSLI GKGCMC 4 cut(s) 384, 1242, 1296, 3302
BstV1I GCAGC 5 cut(s) 73, 90, 676, 1861, 1880
BstV2I GAAGAC 3 cut(s) 1752, 1858, 3077
BstX2I RGATCY 5 cut(s) 398, 2000, 2296, 2408, 2431
BstXI CCANNNNNNTGG 2 cut(s) 1382, 2030
BstYI RGATCY 5 cut(s) 398, 2000, 2296, 2408, 2431
BstZ17I GTATAC 2 cut(s) 2633, 3260
BstZI CGGCCG 1 cut(s) 1774
Bsu36I CCTNAGG 2 cut(s) 2169, 3406
BsuI GTATCC 1 cut(s) 482
BsuRI GGCC 7 cut(s) 382, 716, 1240, 1331, 1776, 2022, 3585
BtgI CCRYGG 1 cut(s) 2818
BtsCI GGATG 8 cut(s) 487, 766, 1633, 2296, 2482, 2732, 3118, 3148
BtsI GCAGTG 1 cut(s) 3892
BtsIMutI CAGTG 6 cut(s) 1234, 1612, 2498, 2722, 3174, 3892
BveI ACCTGC 1 cut(s) 2940
Cac8I GCNNGC 5 cut(s) 2647, 2974, 3070, 3273, 3493
CaiI CAGNNNCTG 1 cut(s) 2961
CciI TCATGA 3 cut(s) 1555, 1699, 3870
CfoI GCGC 3 cut(s) 983, 1481, 3465
Cfr13I GGNCC 9 cut(s) 148, 380, 381, 939, 1238, 1239, 1329, 2952, 3306
CseI GACGC 1 cut(s) 2691
Csp6I GTAC 5 cut(s) 1631, 2165, 2597, 3179, 3206
CviQI GTAC 5 cut(s) 1631, 2165, 2597, 3179, 3206
DinI GGCGCC 2 cut(s) 1480, 3464
DraI TTTAAA 2 cut(s) 3766, 3846
DraIII CACNNNGTG 2 cut(s) 1364, 3109
DriI GACNNNNNGTC 1 cut(s) 342
EaeI YGGCCR 4 cut(s) 714, 1774, 2020, 3583
EagI CGGCCG 1 cut(s) 1774
Eam1104I CTCTTC 7 cut(s) 540, 642, 822, 1050, 1385, 1880, 1953
Eam1105I GACNNNNNGTC 1 cut(s) 342
EarI CTCTTC 7 cut(s) 540, 642, 822, 1050, 1385, 1880, 1953
EclXI CGGCCG 1 cut(s) 1774
Eco130I CCWWGG 4 cut(s) 385, 942, 1808, 2818
Eco24I GRGCYC 6 cut(s) 74, 384, 1242, 1807, 2793, 3744
Eco31I GGTCTC 2 cut(s) 177, 3654
Eco32I GATATC 1 cut(s) 562
Eco47I GGWCC 4 cut(s) 148, 939, 2952, 3306
Eco52I CGGCCG 1 cut(s) 1774
Eco57I CTGAAG 6 cut(s) 543, 588, 759, 1538, 2367, 2954
Eco81I CCTNAGG 2 cut(s) 2169, 3406
EcoNI CCTNNNNNAGG 1 cut(s) 924
EcoO109I RGGNCCY 4 cut(s) 381, 939, 1239, 1329
EcoRI GAATTC 1 cut(s) 2338
EcoRII CCWGG 5 cut(s) 305, 460, 716, 2368, 2434
EcoRV GATATC 1 cut(s) 562
EcoT14I CCWWGG 4 cut(s) 385, 942, 1808, 2818
EcoT22I ATGCAT 3 cut(s) 793, 3155, 3904
EcoT38I GRGCYC 6 cut(s) 74, 384, 1242, 1807, 2793, 3744
EgeI GGCGCC 2 cut(s) 1480, 3464
EheI GGCGCC 2 cut(s) 1480, 3464
ErhI CCWWGG 4 cut(s) 385, 942, 1808, 2818
Esp3I CGTCTC 3 cut(s) 890, 1587, 2714
FalI AAGNNNNNCTT 2 cut(s) 2235, 2267
FaqI GGGAC 5 cut(s) 431, 1228, 2431, 2625, 2943
FauI CCCGC 1 cut(s) 2650
FauNDI CATATG 2 cut(s) 957, 2191
FbaI TGATCA 2 cut(s) 3058, 3100
FblI GTMKAC 2 cut(s) 2632, 3259
Fnu4HI GCNGC 7 cut(s) 62, 104, 690, 1774, 1783, 1875, 1894
FokI GGATG 8 cut(s) 494, 773, 1640, 2303, 2489, 2719, 3125, 3155
FriOI GRGCYC 6 cut(s) 74, 384, 1242, 1807, 2793, 3744
Fsp4HI GCNGC 7 cut(s) 62, 104, 690, 1774, 1783, 1875, 1894
FspBI CTAG 7 cut(s) 186, 572, 1538, 1665, 2055, 2829, 2882
GlaI GCGC 3 cut(s) 982, 1480, 3464
GluI GCNGC 7 cut(s) 62, 104, 690, 1774, 1783, 1875, 1894
GsaI CCCAGC 1 cut(s) 223
GsuI CTGGAG 4 cut(s) 1296, 1517, 2574, 3728
HaeII RGCGCY 2 cut(s) 1482, 3466
HaeIII GGCC 7 cut(s) 382, 716, 1240, 1331, 1776, 2022, 3585
HapII CCGG 3 cut(s) 290, 934, 2474
HgaI GACGC 1 cut(s) 2691
HhaI GCGC 3 cut(s) 983, 1481, 3465
Hin1I GRCGYC 2 cut(s) 1479, 3463
Hin6I GCGC 3 cut(s) 981, 1479, 3463
HinP1I GCGC 3 cut(s) 981, 1479, 3463
HincII GTYRAC 3 cut(s) 31, 1603, 3187
HindII GTYRAC 3 cut(s) 31, 1603, 3187
HindIII AAGCTT 1 cut(s) 624
HpaII CCGG 3 cut(s) 290, 934, 2474
Hpy99I CGWCG 1 cut(s) 3462
HpyCH4IV ACGT 3 cut(s) 2723, 3214, 3457
HpyF10VI GCNNNNNNNGC 8 cut(s) 1779, 1782, 2546, 2973, 3272, 3748, 3821, 3848
HpySE526I ACGT 3 cut(s) 2723, 3214, 3457
Hsp92I GRCGYC 2 cut(s) 1479, 3463
HspAI GCGC 3 cut(s) 981, 1479, 3463
KasI GGCGCC 2 cut(s) 1478, 3462
Kpn2I TCCGGA 2 cut(s) 933, 2473
KpnI GGTACC 1 cut(s) 2168
Ksp22I TGATCA 2 cut(s) 3058, 3100
LmnI GCTCC 6 cut(s) 641, 1770, 1810, 2284, 2788, 3644
Lsp1109I GCAGC 5 cut(s) 73, 90, 676, 1861, 1880
MaeI CTAG 7 cut(s) 186, 572, 1538, 1665, 2055, 2829, 2882
MaeII ACGT 3 cut(s) 2723, 3214, 3457
MbiI CCGCTC 1 cut(s) 1773
MfeI CAATTG 1 cut(s) 2388
MflI RGATCY 5 cut(s) 398, 2000, 2296, 2408, 2431
MhlI GDGCHC 9 cut(s) 74, 384, 1242, 1296, 1807, 2793, 3231, 3302, 3744
MlsI TGGCCA 3 cut(s) 716, 2022, 3585
MluNI TGGCCA 3 cut(s) 716, 2022, 3585
Mly113I GGCGCC 2 cut(s) 1479, 3463
MlyI GAGTC 4 cut(s) 56, 352, 356, 1783
MmeI TCCRAC 3 cut(s) 499, 3369, 3772
Mox20I TGGCCA 3 cut(s) 716, 2022, 3585
Mph1103I ATGCAT 3 cut(s) 793, 3155, 3904
MroI TCCGGA 2 cut(s) 933, 2473
MroXI GAANNNNTTC 3 cut(s) 656, 772, 3084
MscI TGGCCA 3 cut(s) 716, 2022, 3585
MslI CAYNNNNRTG 4 cut(s) 270, 1287, 3816, 3942
Msp20I TGGCCA 3 cut(s) 716, 2022, 3585
MspA1I CMGCKG 2 cut(s) 2345, 3266
MspI CCGG 3 cut(s) 290, 934, 2474
MspR9I CCNGG 6 cut(s) 291, 307, 462, 718, 2370, 2436
MunI CAATTG 1 cut(s) 2388
Mva1269I GAATGC 3 cut(s) 774, 3040, 3624
MvaI CCWGG 5 cut(s) 307, 462, 718, 2370, 2436
MwoI GCNNNNNNNGC 8 cut(s) 1779, 1782, 2546, 2973, 3272, 3748, 3821, 3848
NarI GGCGCC 2 cut(s) 1479, 3463
NciI CCSGG 1 cut(s) 291
NcoI CCATGG 1 cut(s) 2818
NdeI CATATG 2 cut(s) 957, 2191
NmuCI GTSAC 2 cut(s) 3410, 3427
NsiI ATGCAT 3 cut(s) 793, 3155, 3904
NspI RCATGY 5 cut(s) 795, 1349, 3072, 3815, 3906
PaeI GCATGC 1 cut(s) 3072
PagI TCATGA 3 cut(s) 1555, 1699, 3870
PctI GAATGC 3 cut(s) 774, 3040, 3624
PdmI GAANNNNTTC 3 cut(s) 656, 772, 3084
PflMI CCANNNNNTGG 2 cut(s) 312, 467
PfoI TCCNGGA 1 cut(s) 2434
PkrI GCNGC 7 cut(s) 63, 105, 691, 1775, 1784, 1876, 1895
PleI GAGTC 4 cut(s) 55, 351, 356, 1783
PluTI GGCGCC 2 cut(s) 1482, 3466
PpsI GAGTC 4 cut(s) 55, 351, 356, 1783
Ppu21I YACGTR 1 cut(s) 3215
PpuMI RGGWCCY 1 cut(s) 939
PshBI ATTAAT 1 cut(s) 3378
PsiI TTATAA 1 cut(s) 3444
Psp5II RGGWCCY 1 cut(s) 939
Psp6I CCWGG 5 cut(s) 305, 460, 716, 2368, 2434
PspFI CCCAGC 1 cut(s) 219
PspGI CCWGG 5 cut(s) 305, 460, 716, 2368, 2434
PspOMI GGGCCC 2 cut(s) 380, 1238
PspPI GGNCC 9 cut(s) 148, 380, 381, 939, 1238, 1239, 1329, 2952, 3306
PspPPI RGGWCCY 1 cut(s) 939
PstNI CAGNNNCTG 1 cut(s) 2961
PsuI RGATCY 5 cut(s) 398, 2000, 2296, 2408, 2431
PvuII CAGCTG 2 cut(s) 2345, 3266
RsaI GTAC 5 cut(s) 1632, 2166, 2598, 3180, 3207
RsaNI GTAC 5 cut(s) 1631, 2165, 2597, 3179, 3206
RseI CAYNNNNRTG 4 cut(s) 270, 1287, 3816, 3942
SatI GCNGC 7 cut(s) 62, 104, 690, 1774, 1783, 1875, 1894
Sau96I GGNCC 9 cut(s) 148, 380, 381, 939, 1238, 1239, 1329, 2952, 3306
SchI GAGTC 4 cut(s) 56, 352, 356, 1783
ScrFI CCNGG 6 cut(s) 291, 307, 462, 718, 2370, 2436
SduI GDGCHC 9 cut(s) 74, 384, 1242, 1296, 1807, 2793, 3231, 3302, 3744
SfcI CTRYAG 5 cut(s) 870, 882, 1041, 2425, 3310
SfoI GGCGCC 2 cut(s) 1480, 3464
SinI GGWCC 4 cut(s) 148, 939, 2952, 3306
SmiMI CAYNNNNRTG 4 cut(s) 270, 1287, 3816, 3942
SmlI CTYRAG 2 cut(s) 1973, 3354
SmoI CTYRAG 2 cut(s) 1973, 3354
SpeI ACTAGT 1 cut(s) 571
SphI GCATGC 1 cut(s) 3072
SsiI CCGC 6 cut(s) 1020, 1773, 1783, 2606, 2643, 3209
SspDI GGCGCC 2 cut(s) 1478, 3462
SspI AATATT 2 cut(s) 298, 1049
SspMI CTAG 7 cut(s) 186, 572, 1538, 1665, 2055, 2829, 2882
StyD4I CCNGG 6 cut(s) 289, 305, 460, 716, 2368, 2434
StyI CCWWGG 4 cut(s) 385, 942, 1808, 2818
TaiI ACGT 3 cut(s) 2726, 3217, 3460
TaqI TCGA 4 cut(s) 1765, 3616, 3640, 3718
TauI GCSGC 2 cut(s) 1776, 1785
TscAI CASTG 6 cut(s) 1234, 1612, 2505, 2722, 3181, 3892
TseFI GTSAC 2 cut(s) 3410, 3427
TseI GCWGC 5 cut(s) 61, 103, 689, 1874, 1893
Tsp45I GTSAC 2 cut(s) 3410, 3427
TspGWI ACGGA 3 cut(s) 557, 2053, 3415
TspRI CASTG 6 cut(s) 1234, 1612, 2505, 2722, 3181, 3892
Van91I CCANNNNNTGG 2 cut(s) 312, 467
VneI GTGCAC 2 cut(s) 1292, 3298
VpaK11BI GGWCC 4 cut(s) 148, 939, 2952, 3306
VspI ATTAAT 1 cut(s) 3378
XagI CCTNNNNNAGG 1 cut(s) 924
XapI RAATTY 8 cut(s) 16, 597, 2197, 2338, 2362, 2823, 3367, 3916
XbaI TCTAGA 2 cut(s) 2828, 2881
XceI RCATGY 5 cut(s) 795, 1349, 3072, 3815, 3906
XcmI CCANNNNNNNNNTGG 2 cut(s) 3219, 3593
XmiI GTMKAC 2 cut(s) 2632, 3259
XmnI GAANNNNTTC 3 cut(s) 656, 772, 3084
XspI CTAG 7 cut(s) 186, 572, 1538, 1665, 2055, 2829, 2882
Zsp2I ATGCAT 3 cut(s) 793, 3155, 3904
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.