Rh6DG377300

Histone-lysine N-methyltransferase ASHH2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0007450
Physical Location & Seq
Forward (+)
57167297 .. 57180649
13353 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG377300.1

Sequence Viewer

Length: 6231 bp
ATGGCATCGTATGGAAACTTGATGTTCGTGGACGAGCCTCGGTGCGAGTCGTTTGGTACCGAGCAGCAGATGGGCTCGGAGATCTTGGTGGAAAGTGTTTCTGAGCAGAACAACTGCTTGGAGGCTCAGCCTTTACTTGATCGTAATGTGGACCTTACCAGTGAGGCGGACGTGTCTCCGGGGTTTTGTAGGAATGACAATGCTGGTTGTGTGAGCTGCAGTGAGGTGACTGAGTTGGGTACTGGTGATAGAGATGCTTTGGTGGGTGGGGGTGAAAGTACGAGTGTTCTTGAGCCGGAAGATTTTTTGAACAGTAATAGGTGTGGCTTGGATGAGAATCAGAATGATGGGGATGGTTGTTCTAATATTGGGATGCAGTTGAAGGAAGGCTGGTGTCGTGAGGATGGCTTTGCCGGTTCATTTGATGATTGTGAAGTGCCCTCGAAAGTCATACCTTCCACAGGGTCACCAGGAAATGGTATTCAACTGGATGAGCACAGGGATGATAAAAGTGCTAATCGTCTGTCTTCTGAAGGGATGATGGAAGTTATGGAAGAGCAAGGTGAACTGCCCTTGGAATTTGTAGACATTGATGTTTGTAGGGATTACAACATCTTTTCTGGTAATTCTTTGGAAGCTGCTTATGAAAAAAGTGTTGCTTTGACTGGGATAGAAGTTGGTGGTTTGTGCAACGGGATATCACCTGCACGGGATGGTGGAATGCCTTCAGAAGTATCAAACACAGGTGATCTAGTGACTAACTGTAACCGGAATAAAGAACATATTGACGACAATGGTGTCAGTGGTGTAAGCGGCCTTTCTACTGAAAAGTCTACAGAGGTTTCTGAGGCATGCAACCAAATGTTGCCATCACTGAGTTTTCAAAGGTCTTTGGAAGATTTGCACATGCCTAATTCACCGAGTAAGTTTGCCCAGCAGAGGGATTCAAGGAGTAATAATACAGTTGATGGTTCCTTGGCTGATAGGGCTACAGAATTTATGGAAGAGAAAAGTAATGTTATGACAGAGAAGGAGGCTGAAATTCACACCCAGATATCATTTGTAAAAGTTAATGTCTCCTGTTTGAAGGAAAATTCCTCCAATGTAGCTCCCAACTGCATTATTGAGAATTCCGTTCCTCCTCAGTCGTGTGATGCCTTCAGTATTGCTAATAACAGTGTTGATGGACTTTATGCTGATAGTGTTACAGTGGTTGTTGACATGAAAAGCAATATTGTTGATGCATGCAATCAAACATTGCCTCCAGATTTACTGAGTGTTTGCACCCAAGAGAATGATCAGGGTAGTGATAAGGTTAAAGAATGTGTGGAACAGAAAGTTGATGGTACAACTGATATGAGGGTTGAAACTGGCACTCAGATATTACTAAAAGAGGAAAAGACCTCCAATCTCACCAAAGATTCTGCTGGATTATTTCCCAACAGTACTATTGAGAATTCTGTTCCTTTGCAACTGCATCAACCTTTTGATATTGTCAGTAATGACTCTTCTAAGACGGTCGATGTTCCAGATATCAATGATTCTCCTGGTCATGTTGAATCTAGTACTTCATTTGACCATCCTGGACTGATGGACCGTGAAGGAAACGATAATTTCAGAGTTGAGTTTTTTTCTAAAACTGATTTTTCTGAAATTATTGCATTGCCTGCTCAAAGGAGTGGCCGAAGCCGCAAGACTCCGACAAAAAAGGCTCCAAGGAAACGCAGGAATGCATCTAAAGTGTTACAACCTCTTGGAAGTGTTGAAAGTGTCTTCAAAGGTGCTGGAAGGAAGAGAAGCTGCCTTTCCAAACCACCTCGTTCTTCTACCTGGGGATTATTGGGAAGTGTCACTCAATCCTTTGAGGAGAGTAATGGGCTTCAGGTCCATCAGGTTTGTCAAGGTCAGAACGAAGGATCACAGAAACGAAGGGGTGGTCGAAGAAGTGGAAAACAGAAGCAAAGTGTGGCTAGTGGAAATCTTGAAAGGTCTAGGGGAACTTCAACCAATCATGTTCGTTTGAAGGTTAAATTTGGGAAAGAAGTTGATAAAAATTTACTGTATAACAAAGCAGCTGAGTTTGTTGATACCTCAGCATGTGCAAATTCTATTCAAATAGTGAATGTTGTTGAAGGTAATTGGAGACCAGAAGCGACTGTTAGAAAGTGTCAGCATACAAACAAGAAACTAGAGAAGGATGAAACTTGCCAAGATGGAGAACTGGCTGATAAGGACTTGGAGGCTGTTTCTGTTACAGATATTTCAGCTGAAGATGAGATACAGAATTGTCTTGGGGTTCCCTCTCATGCAATTGCTATATCATCAGGAGGATCAGTTGGGAGTAGCTATAGAGACCCTGGAACTTCACCTGATTCTGAAGTTATCAATTTAATTCCAGAAGCACATGTGGAGGCAAGGCCACAAGAAGATTCTCATGGTACTGATTTGACTTCAGACAAAGTTTTATCTGCTTCTGGAGACTTTATTAGCAGTAAGAGAGGAAAGAAGAAACATAAACTCCATTGTGAGGGAAATTGTGTTCCGGATGATGGTTCACCGTGTCCATGCCCAGCTAGCACAATGAGAGCAAAACCATCAACACGTGATGTATGTAGACAGAATGGTAGTCAAGATATTTGCTCTGGCGAGACTTTTACTTCATCCCCAGGTGCCAAGGCGTCAACTAACTCATCAAGTGACAGGGACTTGTCTGTGGAACCCCTGTGCTTGTCAGGAGGAAGTGACCATGGAGTGGAGAGGGGTGCAGAAGCTGAGACAGATTGCATTCTAGATGTGGGCTTAGGTTTACATAGTTCCCAGAATATGCTTCCTTCCTCTAACACCAAGGGGCAGAAGCCTCCCAAGGGCAAATCCAGAGGTTCTGACTCAGCGAGTAAGAGAAGTAATACTCGTAAACCAAAGGAAAAAGAGCAGAAGTCTGTTAATAAGAAAAAAGTCAAGGAGGACAAACAGCTTGCCTGCAAGGTGGAAAGTCTCCCTGAATCAGGAGATCTCTTAGCAGATGCTAACACTTCCCATGTTGCTGAGTGCATGGGTGTTCCTAACTTGGACGTGGTACCTGTTGACTTAGATAAGCAGTATGTACCCCCTCGGAATGCGTGGGTGCTCTGTGATGCTTGTAATAAATGGAGGCGTATACCAGCTGAACTTGCAGATTTTATAGATGAAACAAAGTGCACATGGACCTGTAGAGAGAATCAGGACAGAAACTTTGCTGATTGCTCAATTCGCCAAGAGAAGTCAAATGCAGAAATTAATGCAGAGTTGGAAATATCTGATGCTTCAGGCGAAGAAGATGCTTCTGGCACCCGATTACATTATAAAACATTGGAATGCCGTCGTCCGTCAGTTTCCCAACAGAATGTTGCATCTATTAAAACCAATCAGTTCCTTCACCGTAACCGTAAAAATCAGAGTATTGATGAGATAATGGTTTGCCATTGCAAACCACCTAAAGATGGCCAGTTGGGTTGTGGAGAAGATTGCCTGAATCGAATGCTTAACATCGAATGTGTTCGAGGAACCTGTCCGTGTGGAGATCTTTGTTCAAATCAACAGTTCCAAAAACGCCGATATTCCAAACTAGAGAAGTTTCGGAGTGGGAAGAAGGGGTTTGGGCTCAGATCGCTCGAGTATATATGTAAAGGGCAGTTTCTTATTGAATATGTTGGAGAGGTGCTTGATACGCATGCTTATGAGGCACGACAAAAAGAGTACGCTGTAAAGGGTCACAGGCATTTCTACTTCATGACATTGAATACCAGTGAGGTAATAGATGCATGTGCAAAGGGAAATTTGGGGCGCTTCATCAACCATAGTTGCGTTCCGAACTGTCGCACCGAGAAGTGGATGGTGAATGGAGAGGTCTGTATAGGACTATTTGCACTAAGAGATATAAAGAAGGGTGAAGAGGTGACATTTGACTATAACTTCGTAAGGGTGATTGGAGCTGCTGCAAAGAAATATGAAGAATATGTGGAGCCTGTGATGATCCCAGAAGATGGTGGAGCGGAAGATAGTAGAGGCAATGTGGTGGCCAGACTCGATTCCTTAGATGGTGCGATAATTCAACGTGAGGAGAGTGCATCTACAAATAAAGACATTGATAAATCAACAATTTCTGTTGGTAAATTGGAGATTACCACACAGAGAGAGGAGTCTCTGGAATTGCAGCACTTGTTGCCATCTTTTGTCCAACCAGTTGAAGCGTTCCAACAAACAGAAGATGTAACTAGTAGATCCACGCCTGTTGTTCGGCAGGAGGTTTCCAGGGAAAAGGGGACTACAGAAAAATCCTCAGACTCCTTTGAAAGACCAGAGATTACTTCTCCAACAAAAGTGATTAGCAAACCTTTATCTGATGATATTGATGCTAACAAAAACTCCAAGTTTAACACTATTGAAGATGAGCAGCTTTCCTCTAAAGTGCATCGGAATGTGAAAACTTCTCGTTCATCCAGTTCTGTGAAGAAAGGCAAAGTTAGGAGTACTCCTCTAAACACAAACAAAATTCAGGTGGTAGCCAATAAATCTCATGTGCTACCTTTTAAACCGAAAAGATCAATCGAAGGATCAGTTGAAGAAAAGCTTAACGAGCTGTTGGATGTTGATGGAGGGATAAGTAAACGCAAAGATTCCACTAAAGGCTACTTAAAGCTTCTGTTCCTTACCGCACAATCTGGTGATAGTGGCAGTGGGGAAGCAATTAAAAGCAATCGAGATCTTTCGATTATTCTTGATGCGCTTTTGAAGACAAAGTCGCGTACGGTTTTGATTGATATTATTAACAAAAATGGTTTGAGAATGCTACACAACATTATGAAGATGTGCAGGAGAGACTTTAACAAGATTCCGATCCTCCGGAAGCTTTTGAAGGTCCTAGAATATTTGGCCGAAAAGCCGCAGATTCTTACACAGGAACATATTACTGGAGGTCCTCCCTGCCCGGGAATGGAGAGCTTTACAGAGTCGATACTATCACTGACGGAGCATGGTGACAAAAGGGTCCATGACATAGCACGCAACTTCCGAAATAGGTGGATTCCAAAAGCTTTCAGAAGACATAGTTTTGTAGACAGGGATGACGGAAAGATGGAATTTAACAGGAGTTCAAACTACAATAGGTTTTCCACAACACATGATACATGGCGTGATCAAAGTGGAAGATCTACTGAAGTTGCAGATAATGCCAAGCAATCAGTGGTTAAAACACCAACTTCTGCGAGTACGGTTATCCAGGATGGCTCTTCTACACCTTCTACAGCTGGTTGTGCAACCAGTGAGACAAAAGTTCGCAAGCGTAAAAGCCGATGGGATCAGCCAGCAGTGACAGTCCTTGATCCAAGTTCACTTCCAAACAAGGAACAGAAGATCGAGTCTAAACAGCTAGAATCTAGTCCAGTACCTCGGATAGGTGATGTGACATCGCACCCTGAAAATGGGAGCAGAGAGGGCGGAAATCGCACCAGCACTTTGCATGATTTATGTCAGCAAGTTGGTGCTGATGTGGTTTATGATGGAGAGCAAAACATTCCTGAGGATGTTCCTCCTGGATTTTCATCTTGCCTTAATACCCCTGTGGTTTCGTCAACATCTTCAGTTATAGGGCATCCGCAGGCCAAGTTTGTTTCTCGCTTACCTGTCTCGTATGGTATTCCACTGTCTATCATGCAGCAATATGGGACACCCCATGCTGAAAGTGTTGATACTTGGGTTGTTGCTCCTGGCATGCCTTTCCATCCTTTTCCACCATTGCCCCCATGTCCCCGTCGTAAGAAAGACCCTTCTCATGATGTCAATCATGTGTCAGTTAACCAAGCTTCAGAAGGACAACAAGCCAGCTGCGTTGCTACGAATTGTCACTCTGAAGAAAGCAGTCCAAGCACAACCGGTGCCACTCAAGCAGACTTTGGCACACCATGTGCAAATAATCAATTGGGTACTAAACGACAGAGGGAGTCCTCATATGAGGCCCCCTTGGGGAGGAGATACTTCAAGCAGCAGAAGTGGAATCATGCAAAACCTAGGCTCCCATGGGTTAGGGATAGGAATGGTTGGGGATGTAATGGGAACAACTTCAGAGGTGGGACAAACAGCATAGGCGTAGGAACTGTAGGAAATGAGCTTAGAACTCCATATTGTTCAGAGGACATGAGCTATAGGGTGGAGAAAGCTGGCAATAATGTCAATCAGCATTCACATCACCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001763 GO:0002682 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006464 GO:0006479 GO:0006629 GO:0006720 GO:0006721 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008213 GO:0008276 GO:0008757 GO:0009553 GO:0009555 GO:0009653 GO:0009791 GO:0009893 GO:0009908 GO:0009909 GO:0009910 GO:0009987 GO:0010016 GO:0010223 GO:0010346 GO:0010363 GO:0010452 GO:0010468 GO:0010604 GO:0010638 GO:0010941 GO:0016043 GO:0016108 GO:0016116 GO:0016278 GO:0016279 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018022 GO:0018024 GO:0018193 GO:0018205 GO:0019222 GO:0019538 GO:0022414 GO:0031056 GO:0031058 GO:0031060 GO:0031062 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0032259 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033043 GO:0033044 GO:0034968 GO:0035670 GO:0036211 GO:0040029 GO:0042054 GO:0042800 GO:0043067 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044255 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045088 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048443 GO:0048466 GO:0048467 GO:0048481 GO:0048518 GO:0048519 GO:0048522 GO:0048580 GO:0048581 GO:0048583 GO:0048608 GO:0048646 GO:0048653 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0050776 GO:0050789 GO:0050793 GO:0050794 GO:0051093 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051239 GO:0051241 GO:0051246 GO:0051247 GO:0051276 GO:0051568 GO:0060255 GO:0061458 GO:0065007 GO:0071704 GO:0071840 GO:0080090 GO:0080134 GO:0080135 GO:0090567 GO:0099402 GO:0140096 GO:1901564 GO:1902275 GO:1905269 GO:1905393 GO:2000026 GO:2000241 GO:2000242 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko00310 map00310
Pfam Domains
Protein Families
SET zf-CW

Protein Analysis

2076

Amino Acids

227.83

Weight (kDa)

5.85

Isoelectric Point (pI)

48.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CW PF07496 1041 - 1087 3.9e-10 CW-type Zinc Finger
AWS PF17907 1162 - 1197 1.8e-14 AWS domain
SET PF00856 1211 - 1317 1.3e-24 SET domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3342
AarI CACCTGC 1 cut(s) 712
AasI GACNNNNNNGTC 2 cut(s) 797, 5024
Acc36I ACCTGC 1 cut(s) 712
Acc65I GGTACC 2 cut(s) 56, 3076
AccB1I GGYRCC 5 cut(s) 56, 2669, 3076, 3326, 5912
AccB7I CCANNNNNTGG 3 cut(s) 476, 2752, 4022
AccBSI CCGCTC 1 cut(s) 4031
AccI GTMKAC 5 cut(s) 585, 833, 2614, 3157, 5094
AccII CGCG 1 cut(s) 4783
AccIII TCCGGA 2 cut(s) 2542, 4881
AciI CCGC 8 cut(s) 167, 813, 1690, 4031, 4692, 4922, 5475, 5633
AclWI GGATC 8 cut(s) 1924, 2338, 4006, 4254, 4600, 4870, 5343, 5354
AcoI YGGCCR 4 cut(s) 1681, 3481, 4056, 4911
AcvI CACGTG 1 cut(s) 2603
AcyI GRCGYC 1 cut(s) 2678
AdeI CACNNNGTG 1 cut(s) 5942
AflIII ACRYGT 3 cut(s) 171, 2404, 2600
AgeI ACCGGT 1 cut(s) 5909
AhlI ACTAGT 1 cut(s) 4253
AjiI CACGTC 2 cut(s) 172, 3073
AjuI GAANNNNNNNTTGG 2 cut(s) 101, 133
AloI GAACNNNNNNTCC 2 cut(s) 2523, 2555
Alw21I GWGCWC 3 cut(s) 498, 3129, 3200
Alw44I GTGCAC 1 cut(s) 3196
AlwI GGATC 8 cut(s) 1924, 2338, 4006, 4254, 4600, 4870, 5343, 5354
AlwNI CAGNNNCTG 1 cut(s) 2771
Ama87I CYCGRG 2 cut(s) 3650, 4966
Aor13HI TCCGGA 2 cut(s) 2542, 4881
AoxI GGCC 8 cut(s) 814, 1681, 2417, 3481, 4056, 4911, 5637, 5991
ApaLI GTGCAC 1 cut(s) 3196
ArsI GACNNNNNNTTYG 6 cut(s) 1921, 1953, 3215, 3247, 3935, 3967
AseI ATTAAT 1 cut(s) 3276
AsiGI ACCGGT 1 cut(s) 5909
Asp700I GAANNNNTTC 3 cut(s) 724, 3534, 6095
Asp718I GGTACC 2 cut(s) 56, 3076
AspA2I CCTAGG 1 cut(s) 6044
AspLEI GCGC 2 cut(s) 3825, 4765
AspS9I GGNCC 8 cut(s) 151, 1594, 1885, 3204, 4897, 4955, 5026, 5992
AsuC2I CCSGG 3 cut(s) 180, 4967, 4968
AsuNHI GCTAGC 1 cut(s) 2573
AvaI CYCGRG 2 cut(s) 3650, 4966
AvaII GGWCC 7 cut(s) 151, 1594, 1885, 3204, 4897, 4955, 5026
AvrII CCTAGG 1 cut(s) 6044
AxyI CCTNAGG 1 cut(s) 5556
BaeGI GKGCMC 2 cut(s) 441, 3200
BaeI ACNNNNGTAYC 2 cut(s) 5718, 5751
BalI TGGCCA 2 cut(s) 3483, 4058
BanI GGYRCC 5 cut(s) 56, 2669, 3076, 3326, 5912
BanII GRGCYC 2 cut(s) 77, 3642
BarI GAAGNNNNNNTAC 4 cut(s) 1492, 1524, 2262, 2294
BbrPI CACGTG 1 cut(s) 2603
BbsI GAAGAC 4 cut(s) 519, 1765, 4778, 5086
Bbv12I GWGCWC 3 cut(s) 498, 3129, 3200
BbvCI CCTCAGC 1 cut(s) 2092
BceAI ACGGC 1 cut(s) 3342
BcgI CGANNNNNNTGC 2 cut(s) 3299, 3333
BclI TGATCA 2 cut(s) 1297, 5173
BcnI CCSGG 3 cut(s) 180, 4967, 4968
BcuI ACTAGT 1 cut(s) 4253
BfmI CTRYAG 9 cut(s) 217, 834, 990, 2347, 3208, 4305, 5280, 6132, 6178
BfoI RGCGCY 1 cut(s) 3826
BfuAI ACCTGC 1 cut(s) 712
BglII AGATCT 5 cut(s) 81, 3010, 3559, 4741, 5186
BlnI CCTAGG 1 cut(s) 6044
BlpI GCTNAGC 1 cut(s) 126
BmcAI AGTACT 3 cut(s) 1447, 1567, 4510
Bme18I GGWCC 7 cut(s) 151, 1594, 1885, 3204, 4897, 4955, 5026
BmeT110I CYCGRG 2 cut(s) 3650, 4966
BmgBI CACGTC 2 cut(s) 172, 3073
BmgT120I GGNCC 8 cut(s) 151, 1594, 1885, 3204, 4897, 4955, 5026, 5992
BmrI ACTGGG 1 cut(s) 675
BmtI GCTAGC 1 cut(s) 2577
BmuI ACTGGG 1 cut(s) 675
BpiI GAAGAC 4 cut(s) 519, 1765, 4778, 5086
BplI GAGNNNNNCTC 2 cut(s) 4498, 4530
BpmI CTGGAG 3 cut(s) 1248, 2496, 4971
Bpu10I CCTNAGC 2 cut(s) 2092, 2800
Bpu1102I GCTNAGC 1 cut(s) 126
BpuEI CTTGAG 2 cut(s) 311, 5904
BpuMI CCSGG 3 cut(s) 180, 4967, 4968
BsaAI YACGTR 1 cut(s) 2603
BsaBI GATNNNNATC 4 cut(s) 336, 4874, 5578, 5817
BsaHI GRCGYC 1 cut(s) 2678
BsaI GGTCTC 2 cut(s) 2137, 2346
BsaWI WCCGGW 4 cut(s) 768, 2542, 4881, 5909
Bse118I RCCGGY 2 cut(s) 413, 5909
Bse21I CCTNAGG 1 cut(s) 5556
Bse3DI GCAATG 5 cut(s) 1256, 1661, 3460, 4054, 5771
Bse8I GATNNNNATC 4 cut(s) 336, 4874, 5578, 5817
BseAI TCCGGA 2 cut(s) 2542, 4881
BseJI GATNNNNATC 4 cut(s) 336, 4874, 5578, 5817
BseMI GCAATG 5 cut(s) 1256, 1661, 3460, 4054, 5771
BseRI GAGGAG 6 cut(s) 1131, 1880, 4112, 4190, 4503, 6019
BseSI GKGCMC 2 cut(s) 441, 3200
BseYI CCCAGC 2 cut(s) 933, 2569
BsgI GTGCAG 3 cut(s) 690, 2784, 4870
Bsh1236I CGCG 1 cut(s) 4783
Bsh1285I CGRYCG 1 cut(s) 1521
BshFI GGCC 8 cut(s) 816, 1683, 2419, 3483, 4058, 4913, 5639, 5993
BshNI GGYRCC 5 cut(s) 56, 2669, 3076, 3326, 5912
BshTI ACCGGT 1 cut(s) 5909
BsiEI CGRYCG 1 cut(s) 1521
BsiHKAI GWGCWC 3 cut(s) 498, 3129, 3200
BsiHKCI CYCGRG 2 cut(s) 3650, 4966
BsiSI CCGG 8 cut(s) 179, 296, 414, 769, 2543, 4882, 4967, 5910
BsiWI CGTACG 1 cut(s) 4784
BslFI GGGAC 5 cut(s) 2717, 4315, 5716, 5769, 6121
BsmFI GGGAC 5 cut(s) 2717, 4315, 5716, 5769, 6121
BsmI GAATGC 8 cut(s) 726, 1735, 2784, 3121, 3359, 3522, 4830, 6214
BsnI GGCC 8 cut(s) 816, 1683, 2419, 3483, 4058, 4913, 5639, 5993
Bso31I GGTCTC 2 cut(s) 2137, 2346
BsoBI CYCGRG 2 cut(s) 3650, 4966
Bsp1286I GDGCHC 6 cut(s) 77, 441, 498, 3129, 3200, 3642
Bsp13I TCCGGA 2 cut(s) 2542, 4881
Bsp1720I GCTNAGC 1 cut(s) 126
Bsp19I CCATGG 2 cut(s) 2746, 6053
BspACI CCGC 8 cut(s) 167, 813, 1690, 4031, 4692, 4922, 5475, 5633
BspANI GGCC 8 cut(s) 816, 1683, 2419, 3483, 4058, 4913, 5639, 5993
BspEI TCCGGA 2 cut(s) 2542, 4881
BspFNI CGCG 1 cut(s) 4783
BspHI TCATGA 2 cut(s) 3768, 5809
BspMAI CTGCAG 1 cut(s) 221
BspMI ACCTGC 1 cut(s) 712
BspOI GCTAGC 1 cut(s) 2577
BspPI GGATC 8 cut(s) 1924, 2338, 4006, 4254, 4600, 4870, 5343, 5354
BspQI GCTCTTC 2 cut(s) 549, 5272
BspT107I GGYRCC 5 cut(s) 56, 2669, 3076, 3326, 5912
BspTNI GGTCTC 2 cut(s) 2137, 2346
BsrBI CCGCTC 1 cut(s) 4031
BsrDI GCAATG 5 cut(s) 1256, 1661, 3460, 4054, 5771
BsrFI RCCGGY 2 cut(s) 413, 5909
BssAI RCCGGY 2 cut(s) 413, 5909
BssNAI GTATAC 1 cut(s) 3158
BssNI GRCGYC 1 cut(s) 2678
Bst1107I GTATAC 1 cut(s) 3158
Bst6I CTCTTC 6 cut(s) 549, 999, 1513, 1787, 3924, 5272
BstACI GRCGYC 1 cut(s) 2678
BstAPI GCANNNNNTGC 3 cut(s) 1667, 4201, 5207
BstBAI YACGTR 1 cut(s) 2603
BstDSI CCRYGG 2 cut(s) 2746, 6053
BstEII GGTNACC 1 cut(s) 465
BstENI CCTNNNNNAGG 4 cut(s) 459, 3003, 5430, 6001
BstFNI CGCG 1 cut(s) 4783
BstH2I RGCGCY 1 cut(s) 3826
BstHHI GCGC 2 cut(s) 3825, 4765
BstMCI CGRYCG 1 cut(s) 1521
BstNSI RCATGY 8 cut(s) 855, 910, 1248, 2100, 2408, 3713, 3804, 5752
BstPI GGTNACC 1 cut(s) 465
BstSFI CTRYAG 9 cut(s) 217, 834, 990, 2347, 3208, 4305, 5280, 6132, 6178
BstSLI GKGCMC 2 cut(s) 441, 3200
BstUI CGCG 1 cut(s) 4783
BstV2I GAAGAC 4 cut(s) 519, 1765, 4778, 5086
BstX2I RGATCY 6 cut(s) 81, 3010, 3559, 4259, 4741, 5186
BstYI RGATCY 6 cut(s) 81, 3010, 3559, 4259, 4741, 5186
BstZ17I GTATAC 1 cut(s) 3158
Bsu36I CCTNAGG 1 cut(s) 5556
BsuRI GGCC 8 cut(s) 816, 1683, 2419, 3483, 4058, 4913, 5639, 5993
BtgI CCRYGG 2 cut(s) 2746, 6053
BtgZI GCGATG 1 cut(s) 5430
BtrI CACGTC 2 cut(s) 172, 3073
BtsI GCAGTG 3 cut(s) 226, 4720, 5352
BveI ACCTGC 1 cut(s) 712
CaiI CAGNNNCTG 1 cut(s) 2771
CciI TCATGA 2 cut(s) 3768, 5809
CfoI GCGC 2 cut(s) 3825, 4765
Cfr10I RCCGGY 2 cut(s) 413, 5909
Cfr13I GGNCC 8 cut(s) 151, 1594, 1885, 3204, 4897, 4955, 5026, 5992
Cfr9I CCCGGG 1 cut(s) 4966
CseI GACGC 1 cut(s) 2667
CspAI ACCGGT 1 cut(s) 5909
CspCI CAANNNNNGTGG 2 cut(s) 4252, 4287
DraI TTTAAA 1 cut(s) 4570
DraIII CACNNNGTG 1 cut(s) 5942
DrdI GACNNNNNNGTC 2 cut(s) 797, 5024
DseDI GACNNNNNNGTC 2 cut(s) 797, 5024
EaeI YGGCCR 4 cut(s) 1681, 3481, 4056, 4911
Eam1104I CTCTTC 6 cut(s) 549, 999, 1513, 1787, 3924, 5272
EarI CTCTTC 6 cut(s) 549, 999, 1513, 1787, 3924, 5272
EciI GGCGGA 2 cut(s) 182, 5490
Eco24I GRGCYC 2 cut(s) 77, 3642
Eco31I GGTCTC 2 cut(s) 2137, 2346
Eco32I GATATC 3 cut(s) 699, 1056, 1534
Eco47I GGWCC 7 cut(s) 151, 1594, 1885, 3204, 4897, 4955, 5026
Eco72I CACGTG 1 cut(s) 2603
Eco81I CCTNAGG 1 cut(s) 5556
Eco88I CYCGRG 2 cut(s) 3650, 4966
Eco91I GGTNACC 1 cut(s) 465
EcoNI CCTNNNNNAGG 4 cut(s) 459, 3003, 5430, 6001
EcoO109I RGGNCCY 3 cut(s) 4897, 4955, 5992
EcoO65I GGTNACC 1 cut(s) 465
EcoRI GAATTC 2 cut(s) 1129, 1456
EcoRV GATATC 3 cut(s) 699, 1056, 1534
EcoT22I ATGCAT 3 cut(s) 1246, 1735, 3802
EcoT38I GRGCYC 2 cut(s) 77, 3642
FalI AAGNNNNNCTT 4 cut(s) 643, 675, 4593, 4625
FaqI GGGAC 5 cut(s) 2717, 4315, 5716, 5769, 6121
FauNDI CATATG 1 cut(s) 5986
FbaI TGATCA 2 cut(s) 1297, 5173
FblI GTMKAC 5 cut(s) 585, 833, 2614, 3157, 5094
FriOI GRGCYC 2 cut(s) 77, 3642
GlaI GCGC 2 cut(s) 3824, 4764
GsaI CCCAGC 2 cut(s) 937, 2573
GsuI CTGGAG 3 cut(s) 1248, 2496, 4971
HaeII RGCGCY 1 cut(s) 3826
HaeIII GGCC 8 cut(s) 816, 1683, 2419, 3483, 4058, 4913, 5639, 5993
HapII CCGG 8 cut(s) 179, 296, 414, 769, 2543, 4882, 4967, 5910
HgaI GACGC 1 cut(s) 2667
HhaI GCGC 2 cut(s) 3825, 4765
Hin1I GRCGYC 1 cut(s) 2678
Hin6I GCGC 2 cut(s) 3823, 4763
HinP1I GCGC 2 cut(s) 3823, 4763
HincII GTYRAC 5 cut(s) 1219, 2682, 3085, 5610, 5833
HindII GTYRAC 5 cut(s) 1219, 2682, 3085, 5610, 5833
HindIII AAGCTT 5 cut(s) 4607, 4676, 4886, 5070, 5838
HpaI GTTAAC 1 cut(s) 5833
HpaII CCGG 8 cut(s) 179, 296, 414, 769, 2543, 4882, 4967, 5910
Hpy99I CGWCG 2 cut(s) 3363, 5793
HpyCH4IV ACGT 4 cut(s) 171, 2602, 3072, 4093
HpySE526I ACGT 4 cut(s) 171, 2602, 3072, 4093
Hsp92I GRCGYC 1 cut(s) 2678
HspAI GCGC 2 cut(s) 3823, 4763
Kpn2I TCCGGA 2 cut(s) 2542, 4881
KpnI GGTACC 2 cut(s) 60, 3080
Ksp22I TGATCA 2 cut(s) 1297, 5173
KspAI GTTAAC 1 cut(s) 5833
LguI GCTCTTC 2 cut(s) 549, 5272
LmnI GCTCC 9 cut(s) 1114, 1717, 3968, 4000, 4028, 5008, 5463, 5746, 6054
MaeII ACGT 4 cut(s) 171, 2602, 3072, 4093
MbiI CCGCTC 1 cut(s) 4031
MfeI CAATTG 2 cut(s) 2310, 5954
MflI RGATCY 6 cut(s) 81, 3010, 3559, 4259, 4741, 5186
MhlI GDGCHC 6 cut(s) 77, 441, 498, 3129, 3200, 3642
MlsI TGGCCA 2 cut(s) 3483, 4058
MluNI TGGCCA 2 cut(s) 3483, 4058
MmeI TCCRAC 7 cut(s) 1724, 3267, 3670, 4240, 4258, 4376, 4602
Mox20I TGGCCA 2 cut(s) 3483, 4058
Mph1103I ATGCAT 3 cut(s) 1246, 1735, 3802
MroI TCCGGA 2 cut(s) 2542, 4881
MroXI GAANNNNTTC 3 cut(s) 724, 3534, 6095
MscI TGGCCA 2 cut(s) 3483, 4058
MslI CAYNNNNRTG 4 cut(s) 501, 3352, 3714, 4455
Msp20I TGGCCA 2 cut(s) 3483, 4058
MspA1I CMGCKG 5 cut(s) 2075, 2267, 3164, 5285, 5862
MspI CCGG 8 cut(s) 179, 296, 414, 769, 2543, 4882, 4967, 5910
MunI CAATTG 2 cut(s) 2310, 5954
Mva1269I GAATGC 8 cut(s) 726, 1735, 2784, 3121, 3359, 3522, 4830, 6214
MvnI CGCG 1 cut(s) 4783
NciI CCSGG 3 cut(s) 180, 4967, 4968
NcoI CCATGG 2 cut(s) 2746, 6053
NdeI CATATG 1 cut(s) 5986
NheI GCTAGC 1 cut(s) 2573
NsiI ATGCAT 3 cut(s) 1246, 1735, 3802
NspI RCATGY 8 cut(s) 855, 910, 1248, 2100, 2408, 3713, 3804, 5752
PaeI GCATGC 4 cut(s) 855, 1248, 3713, 5752
PaeR7I CTCGAG 1 cut(s) 3650
PagI TCATGA 2 cut(s) 3768, 5809
PaqCI CACCTGC 1 cut(s) 712
PciI ACATGT 1 cut(s) 2404
PciSI GCTCTTC 2 cut(s) 549, 5272
PcsI WCGNNNNNNNCGW 1 cut(s) 5047
PctI GAATGC 8 cut(s) 726, 1735, 2784, 3121, 3359, 3522, 4830, 6214
PdmI GAANNNNTTC 3 cut(s) 724, 3534, 6095
Pfl23II CGTACG 1 cut(s) 4784
PflFI GACNNNGTC 1 cut(s) 4777
PflMI CCANNNNNTGG 3 cut(s) 476, 2752, 4022
PfoI TCCNGGA 3 cut(s) 1582, 5256, 5569
PinAI ACCGGT 1 cut(s) 5909
PmaCI CACGTG 1 cut(s) 2603
PmlI CACGTG 1 cut(s) 2603
Ppu21I YACGTR 1 cut(s) 2603
PpuMI RGGWCCY 2 cut(s) 4897, 4955
PscI ACATGT 1 cut(s) 2404
PshBI ATTAAT 1 cut(s) 3276
PsiI TTATAA 1 cut(s) 3342
Psp5II RGGWCCY 2 cut(s) 4897, 4955
PspCI CACGTG 1 cut(s) 2603
PspEI GGTNACC 1 cut(s) 465
PspFI CCCAGC 2 cut(s) 933, 2569
PspLI CGTACG 1 cut(s) 4784
PspPI GGNCC 8 cut(s) 151, 1594, 1885, 3204, 4897, 4955, 5026, 5992
PspPPI RGGWCCY 2 cut(s) 4897, 4955
PspXI VCTCGAGB 1 cut(s) 3650
PstI CTGCAG 1 cut(s) 221
PstNI CAGNNNCTG 1 cut(s) 2771
PsuI RGATCY 6 cut(s) 81, 3010, 3559, 4259, 4741, 5186
PsyI GACNNNGTC 1 cut(s) 4777
PvuII CAGCTG 5 cut(s) 2075, 2267, 3164, 5285, 5862
RseI CAYNNNNRTG 4 cut(s) 501, 3352, 3714, 4455
SapI GCTCTTC 2 cut(s) 549, 5272
Sau96I GGNCC 8 cut(s) 151, 1594, 1885, 3204, 4897, 4955, 5026, 5992
ScaI AGTACT 3 cut(s) 1447, 1567, 4510
SduI GDGCHC 6 cut(s) 77, 441, 498, 3129, 3200, 3642
SfcI CTRYAG 9 cut(s) 217, 834, 990, 2347, 3208, 4305, 5280, 6132, 6178
Sfr274I CTCGAG 1 cut(s) 3650
SinI GGWCC 7 cut(s) 151, 1594, 1885, 3204, 4897, 4955, 5026
SlaI CTCGAG 1 cut(s) 3650
SmaI CCCGGG 1 cut(s) 4968
SmiMI CAYNNNNRTG 4 cut(s) 501, 3352, 3714, 4455
SmlI CTYRAG 3 cut(s) 290, 3650, 5919
SmoI CTYRAG 3 cut(s) 290, 3650, 5919
SpeI ACTAGT 1 cut(s) 4253
SphI GCATGC 4 cut(s) 855, 1248, 3713, 5752
SsiI CCGC 8 cut(s) 167, 813, 1690, 4031, 4692, 4922, 5475, 5633
SspI AATATT 3 cut(s) 367, 1234, 4907
TaiI ACGT 4 cut(s) 174, 2605, 3075, 4096
TatI WGTACW 3 cut(s) 1445, 1565, 4508
TauI GCSGC 3 cut(s) 816, 1692, 4924
TspGWI ACGGA 5 cut(s) 1123, 3354, 3540, 5021, 5121
TspMI CCCGGG 1 cut(s) 4966
Tth111I GACNNNGTC 1 cut(s) 4777
Van91I CCANNNNNTGG 3 cut(s) 476, 2752, 4022
VneI GTGCAC 1 cut(s) 3196
VpaK11BI GGWCC 7 cut(s) 151, 1594, 1885, 3204, 4897, 4955, 5026
VspI ATTAAT 1 cut(s) 3276
XagI CCTNNNNNAGG 4 cut(s) 459, 3003, 5430, 6001
XbaI TCTAGA 1 cut(s) 2788
XceI RCATGY 8 cut(s) 855, 910, 1248, 2100, 2408, 3713, 3804, 5752
XcmI CCANNNNNNNNNTGG 2 cut(s) 3491, 5218
XhoI CTCGAG 1 cut(s) 3650
XmaI CCCGGG 1 cut(s) 4966
XmaJI CCTAGG 1 cut(s) 6044
XmiI GTMKAC 5 cut(s) 585, 833, 2614, 3157, 5094
XmnI GAANNNNTTC 3 cut(s) 724, 3534, 6095
ZrmI AGTACT 3 cut(s) 1447, 1567, 4510
Zsp2I ATGCAT 3 cut(s) 1246, 1735, 3802
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.