MD08G1162300.v1.1

TLC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0016678
Physical Location & Seq
Forward (+)
19052563 .. 19054789
2227 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1162300.v1.1.491

Sequence Viewer

Length: 594 bp
ATGGGTTATCGACACTCGGAGTTGGTTGGGCATACTTGGCCATGGGCATACCCAGCTCTGTGCTTCTCATGCGGTTACTTTGCATATGGTCAGTGGGATATGCTGCATTATGGGTTATATAGTGGTTGGATCCCTTCCATCCTGATGCATCATCTGGTTCTCCTCATTTGCTTTACTCTTGCTTTGTATCGGAAGGTCACCATCAATTACCTTATTCTCACTCTCATTTGCGAGCTGCATTCTATCTTTCTGCATGTGAGGAAAGCGCAGCGGATGGCTGGCATTCGTGACGCTAGAAACCAAATTGTAAGGGCAGAGTGGGTTCTTAATTGGGTAACCTTCACTTTTGCAAGGACGGCAGCTCACCTTCTCGTCACAGCCAAGCTCTTCTGCGATACTCCAAAATTCGGAAAGGGTATAGAGCTGCCGCTTGCACTGTTTGGGATGTTCAGAATGAACTTTCTGAATGCTTCTCTTGGCATTGACCTCTTCCATGCCTTCAAGAGAGAGAGGAATTCCCGGCATGCTAATGCTTATCATGAATTACAAGGCCAAAACAGAGAGTTGCAAATAATTTTGGTTTCTATTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
TRAM_LAG1_CLN8

Protein Analysis

198

Amino Acids

22.9

Weight (kDa)

9.45

Isoelectric Point (pI)

23.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRAM_LAG1_CLN8 PF03798 9 - 149 2.4e-14 TLC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016678)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21790
fragaria_vesca FvH4_2g26990
malus_domestica MD08G1162300.v1.1 MD15G1347700.v1.1
prunus_persica Prupe.1G495500_v2.0.a1
pyrus_communis pycom15g31170
rosa_chinensis RchiOBHm_Chr6g0295871
rosa_laevigata RLG00000011761
rosa_multiflora Rmu_ssc0000277.1_g000001
rosa_roxburghii Rroxscaffold_7G00172100
rosa_rugosa Rorug06G0254900
rosa_samantha Rh6BG376200 Rh6CG382300
rosa_wichuraiana Rw6G032120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 72, 271, 428
AclWI GGATC 2 cut(s) 124, 137
AcoI YGGCCR 1 cut(s) 38
AcsI RAATTY 2 cut(s) 404, 514
AfiI CCNNNNNNNGG 1 cut(s) 407
AgsI TTSAA 1 cut(s) 502
AluBI AGCT 5 cut(s) 56, 235, 362, 385, 424
AluI AGCT 5 cut(s) 56, 235, 362, 385, 424
AlwI GGATC 2 cut(s) 124, 137
AoxI GGCC 2 cut(s) 38, 550
ApeKI GCWGC 5 cut(s) 103, 235, 268, 359, 424
ApoI RAATTY 2 cut(s) 404, 514
AspLEI GCGC 1 cut(s) 268
AsuC2I CCSGG 1 cut(s) 520
AsuHPI GGTGA 2 cut(s) 190, 356
BalI TGGCCA 1 cut(s) 40
BamHI GGATCC 1 cut(s) 129
BbvI GCAGC 5 cut(s) 90, 222, 280, 371, 411
BccI CCATC 3 cut(s) 146, 209, 268
BceAI ACGGC 1 cut(s) 372
BcnI CCSGG 1 cut(s) 520
BfaI CTAG 1 cut(s) 294
BisI GCNGC 6 cut(s) 104, 236, 269, 360, 425, 428
BlsI GCNGC 6 cut(s) 105, 237, 270, 361, 426, 429
Bme1390I CCNGG 1 cut(s) 520
BmiI GGNNCC 1 cut(s) 131
BmrFI CCNGG 1 cut(s) 520
BmsI GCATC 2 cut(s) 135, 157
BpuMI CCSGG 1 cut(s) 520
BsaJI CCNNGG 1 cut(s) 41
Bsc4I CCNNNNNNNGG 1 cut(s) 407
BseDI CCNNGG 1 cut(s) 41
BseGI GGATG 3 cut(s) 138, 279, 450
BseLI CCNNNNNNNGG 1 cut(s) 407
BseRI GAGGAG 1 cut(s) 152
BseXI GCAGC 5 cut(s) 90, 222, 280, 371, 411
BseYI CCCAGC 1 cut(s) 52
BshFI GGCC 2 cut(s) 40, 552
BsiSI CCGG 1 cut(s) 520
BslI CCNNNNNNNGG 1 cut(s) 407
BsmI GAATGC 3 cut(s) 238, 282, 472
BsnI GGCC 2 cut(s) 40, 552
Bsp143I GATC 1 cut(s) 129
Bsp19I CCATGG 1 cut(s) 41
BspACI CCGC 3 cut(s) 72, 271, 428
BspANI GGCC 2 cut(s) 40, 552
BspHI TCATGA 1 cut(s) 538
BspLI GGNNCC 1 cut(s) 131
BspPI GGATC 2 cut(s) 124, 137
BspQI GCTCTTC 1 cut(s) 392
BssECI CCNNGG 1 cut(s) 41
BssMI GATC 1 cut(s) 129
BssT1I CCWWGG 1 cut(s) 41
Bst4CI ACNGT 1 cut(s) 438
Bst6I CTCTTC 2 cut(s) 392, 494
BstC8I GCNNGC 4 cut(s) 233, 280, 432, 525
BstDSI CCRYGG 1 cut(s) 41
BstEII GGTNACC 2 cut(s) 196, 334
BstF5I GGATG 3 cut(s) 138, 279, 450
BstHHI GCGC 1 cut(s) 268
BstKTI GATC 1 cut(s) 132
BstMBI GATC 1 cut(s) 129
BstMWI GCNNNNNNNGC 4 cut(s) 37, 53, 69, 356
BstNSI RCATGY 2 cut(s) 257, 527
BstPI GGTNACC 2 cut(s) 196, 334
BstSCI CCNGG 1 cut(s) 518
BstV1I GCAGC 5 cut(s) 90, 222, 280, 371, 411
BstX2I RGATCY 1 cut(s) 129
BstYI RGATCY 1 cut(s) 129
BsuRI GGCC 2 cut(s) 40, 552
BtgI CCRYGG 1 cut(s) 41
BtsCI GGATG 3 cut(s) 138, 279, 450
BtsIMutI CAGTG 2 cut(s) 98, 434
Cac8I GCNNGC 4 cut(s) 233, 280, 432, 525
CciI TCATGA 1 cut(s) 538
CfoI GCGC 1 cut(s) 268
CseI GACGC 1 cut(s) 299
CviAII CATG 6 cut(s) 42, 69, 254, 494, 524, 539
CviJI RGCY 9 cut(s) 40, 56, 235, 278, 362, 380, 385, 424, 552
CviKI_1 RGCY 9 cut(s) 40, 56, 235, 278, 362, 380, 385, 424, 552
DpnI GATC 1 cut(s) 131
DpnII GATC 1 cut(s) 129
EaeI YGGCCR 1 cut(s) 38
Eam1104I CTCTTC 2 cut(s) 392, 494
EarI CTCTTC 2 cut(s) 392, 494
Eco130I CCWWGG 1 cut(s) 41
Eco91I GGTNACC 2 cut(s) 196, 334
EcoO65I GGTNACC 2 cut(s) 196, 334
EcoRI GAATTC 1 cut(s) 514
EcoT14I CCWWGG 1 cut(s) 41
EcoT22I ATGCAT 1 cut(s) 150
ErhI CCWWGG 1 cut(s) 41
FaeI CATG 6 cut(s) 45, 72, 257, 497, 527, 542
FatI CATG 6 cut(s) 41, 68, 253, 493, 523, 538
FauNDI CATATG 1 cut(s) 85
Fnu4HI GCNGC 6 cut(s) 104, 236, 269, 360, 425, 428
FokI GGATG 3 cut(s) 125, 286, 457
Fsp4HI GCNGC 6 cut(s) 104, 236, 269, 360, 425, 428
FspBI CTAG 1 cut(s) 294
GlaI GCGC 1 cut(s) 267
GluI GCNGC 6 cut(s) 104, 236, 269, 360, 425, 428
GsaI CCCAGC 1 cut(s) 56
HaeIII GGCC 2 cut(s) 40, 552
HapII CCGG 1 cut(s) 520
HgaI GACGC 1 cut(s) 299
HhaI GCGC 1 cut(s) 268
Hin1II CATG 6 cut(s) 45, 72, 257, 497, 527, 542
Hin6I GCGC 1 cut(s) 266
HinP1I GCGC 1 cut(s) 266
HpaII CCGG 1 cut(s) 520
HphI GGTGA 2 cut(s) 190, 356
Hpy188I TCNGA 5 cut(s) 19, 192, 410, 452, 465
Hpy188III TCNNGA 4 cut(s) 142, 287, 502, 539
HpyAV CCTTC 5 cut(s) 144, 187, 349, 377, 508
HpyCH4III ACNGT 1 cut(s) 438
HpyCH4V TGCA 8 cut(s) 83, 106, 148, 238, 253, 350, 434, 568
HpyF10VI GCNNNNNNNGC 4 cut(s) 37, 53, 69, 356
Hsp92II CATG 6 cut(s) 45, 72, 257, 497, 527, 542
HspAI GCGC 1 cut(s) 266
Kzo9I GATC 1 cut(s) 129
LguI GCTCTTC 1 cut(s) 392
LpnPI CCDG 5 cut(s) 66, 140, 155, 264, 533
Lsp1109I GCAGC 5 cut(s) 90, 222, 280, 371, 411
LweI GCATC 2 cut(s) 135, 157
MaeI CTAG 1 cut(s) 294
MaeIII GTNAC 5 cut(s) 74, 196, 287, 334, 373
MalI GATC 1 cut(s) 131
MboI GATC 1 cut(s) 129
MboII GAAGA 2 cut(s) 379, 481
MflI RGATCY 1 cut(s) 129
MlsI TGGCCA 1 cut(s) 40
MluCI AATT 7 cut(s) 205, 303, 328, 404, 514, 542, 573
MluNI TGGCCA 1 cut(s) 40
MmeI TCCRAC 1 cut(s) 107
MnlI CCTC 4 cut(s) 173, 252, 497, 504
Mox20I TGGCCA 1 cut(s) 40
Mph1103I ATGCAT 1 cut(s) 150
MscI TGGCCA 1 cut(s) 40
MseI TTAA 2 cut(s) 327, 592
MslI CAYNNNNRTG 2 cut(s) 143, 528
Msp20I TGGCCA 1 cut(s) 40
MspA1I CMGCKG 1 cut(s) 271
MspI CCGG 1 cut(s) 520
MspR9I CCNGG 1 cut(s) 520
Mva1269I GAATGC 3 cut(s) 238, 282, 472
MwoI GCNNNNNNNGC 4 cut(s) 37, 53, 69, 356
NciI CCSGG 1 cut(s) 520
NcoI CCATGG 1 cut(s) 41
NdeI CATATG 1 cut(s) 85
NdeII GATC 1 cut(s) 129
NlaIII CATG 6 cut(s) 45, 72, 257, 497, 527, 542
NlaIV GGNNCC 1 cut(s) 131
NmuCI GTSAC 3 cut(s) 196, 287, 373
NsiI ATGCAT 1 cut(s) 150
NspI RCATGY 2 cut(s) 257, 527
PaeI GCATGC 1 cut(s) 527
PagI TCATGA 1 cut(s) 538
PciSI GCTCTTC 1 cut(s) 392
PctI GAATGC 3 cut(s) 238, 282, 472
PkrI GCNGC 6 cut(s) 105, 237, 270, 361, 426, 429
PspEI GGTNACC 2 cut(s) 196, 334
PspFI CCCAGC 1 cut(s) 52
PspN4I GGNNCC 1 cut(s) 131
PsuI RGATCY 1 cut(s) 129
RseI CAYNNNNRTG 2 cut(s) 143, 528
SapI GCTCTTC 1 cut(s) 392
SaqAI TTAA 2 cut(s) 327, 592
SatI GCNGC 6 cut(s) 104, 236, 269, 360, 425, 428
Sau3AI GATC 1 cut(s) 129
ScrFI CCNGG 1 cut(s) 520
SfaNI GCATC 2 cut(s) 135, 157
SmiMI CAYNNNNRTG 2 cut(s) 143, 528
SphI GCATGC 1 cut(s) 527
Sse9I AATT 7 cut(s) 205, 303, 328, 404, 514, 542, 573
SsiI CCGC 3 cut(s) 72, 271, 428
SspMI CTAG 1 cut(s) 294
StyD4I CCNGG 1 cut(s) 518
StyI CCWWGG 1 cut(s) 41
TaaI ACNGT 1 cut(s) 438
TaqI TCGA 1 cut(s) 10
TasI AATT 7 cut(s) 205, 303, 328, 404, 514, 542, 573
TauI GCSGC 1 cut(s) 430
Tru1I TTAA 2 cut(s) 327, 592
Tru9I TTAA 2 cut(s) 327, 592
TscAI CASTG 2 cut(s) 98, 441
TseFI GTSAC 3 cut(s) 196, 287, 373
TseI GCWGC 5 cut(s) 103, 235, 268, 359, 424
Tsp45I GTSAC 3 cut(s) 196, 287, 373
TspDTI ATGAA 2 cut(s) 470, 555
TspRI CASTG 2 cut(s) 98, 441
XapI RAATTY 2 cut(s) 404, 514
XceI RCATGY 2 cut(s) 257, 527
XspI CTAG 1 cut(s) 294
Zsp2I ATGCAT 1 cut(s) 150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.