MD15G1347700.v1.1

TLC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0016678
Physical Location & Seq
Forward (+)
41174250 .. 41176761
2512 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1347700.v1.1.491

Sequence Viewer

Length: 843 bp
ATGATGAGCAGGACAACGAGATGTCCGAAAGAGGACAAGGCCGGGCCTTTCTTCCTGGCCACTCTGGTGCTCTGGTTCGTCTCCGTTCTGTTCGAAATACTCTTCCACCGGCGCTCGGAGCTGCTCGCCGTTGTTGCCGGCTGCTTCTTTTACCAATTCGCCAACTGGGTTGTCCGCAGTTTCGTCTCCCGTGACCCTCTCTTCGTCAACACCTCCGTTTCGCTCCTCCACTCCTCCATAACCTCCGCTTCAGTGGTATTCATTTTGGTTAATCGGTGGTTAGAAAATGGCTCGGTTGGAATGTTTGAGCATGCGGAGTTGGTTGGACGTACTTGGCCATGGGCATACCCGGCTTTGTGCTTCTCATGCGGTTACTTTGCATATGATCAGTGGGATATGCTGCAGTATGGGTTATATAGCGGTTGGATCCCTTCCATCTTGATGCATCATCTGGTTCTCCTAATTTGTTTTACTCTTGCTTTGTATCGGAATGTCACCATCAATTACCTTATTCTCACTCTCATTTGTGAGCTGCACTCTATCTTTCTGCATGTGAGGAAAGTGCGGCGGATGGCAGGCATTCGTGACGCTACAAGCAAAATTGTAAAAGTTGAGTGGGTTCTTAATTGGGTAACCTTCACTCTGGCAAGGACGGCACCTCACATTCTCATCACAGCCAAGCTCTTCTGCGATGCTCCCAAATTCGGAAAGGGTATAGAGCTGCCACTTGCGCTGTTTGGAATGTTCGGAATGAACTTTCTGAATGCTTCTCTTGGCATTGATCTCTTCCATGCTTTCAAGAGAGAGAGGAATCCTCAGCATGCTAATGCTCATCATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
TRAM_LAG1_CLN8

Protein Analysis

281

Amino Acids

32.19

Weight (kDa)

9.12

Isoelectric Point (pI)

36.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRAM_LAG1_CLN8 PF03798 68 - 256 3.9e-19 TLC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016678)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21790
fragaria_vesca FvH4_2g26990
malus_domestica MD08G1162300.v1.1 MD15G1347700.v1.1
prunus_persica Prupe.1G495500_v2.0.a1
pyrus_communis pycom15g31170
rosa_chinensis RchiOBHm_Chr6g0295871
rosa_laevigata RLG00000011761
rosa_multiflora Rmu_ssc0000277.1_g000001
rosa_roxburghii Rroxscaffold_7G00172100
rosa_rugosa Rorug06G0254900
rosa_samantha Rh6BG376200 Rh6CG382300
rosa_wichuraiana Rw6G032120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 655
AciI CCGC 7 cut(s) 175, 246, 314, 369, 420, 565, 568
AclWI GGATC 2 cut(s) 421, 434
AcoI YGGCCR 2 cut(s) 57, 335
AcsI RAATTY 1 cut(s) 701
AcuI CTGAAG 1 cut(s) 234
AfaI GTAC 1 cut(s) 331
AfiI CCNNNNNNNGG 2 cut(s) 115, 704
AgsI TTSAA 1 cut(s) 799
AjnI CCWGG 1 cut(s) 54
AleI CACNNNNGTG 1 cut(s) 65
AluBI AGCT 4 cut(s) 121, 532, 682, 721
AluI AGCT 4 cut(s) 121, 532, 682, 721
Alw21I GWGCWC 1 cut(s) 72
Alw26I GTCTC 2 cut(s) 85, 190
AlwI GGATC 2 cut(s) 421, 434
AoxI GGCC 4 cut(s) 39, 44, 57, 335
ApeKI GCWGC 5 cut(s) 121, 141, 400, 532, 721
ApoI RAATTY 1 cut(s) 701
ArsI GACNNNNNNTTYG 2 cut(s) 185, 217
AspLEI GCGC 2 cut(s) 114, 733
AspS9I GGNCC 1 cut(s) 44
AsuC2I CCSGG 2 cut(s) 43, 350
AsuHPI GGTGA 1 cut(s) 487
AsuII TTCGAA 1 cut(s) 93
BalI TGGCCA 2 cut(s) 59, 337
BamHI GGATCC 1 cut(s) 426
BanI GGYRCC 1 cut(s) 655
Bbv12I GWGCWC 1 cut(s) 72
BbvCI CCTCAGC 1 cut(s) 816
BbvI GCAGC 5 cut(s) 108, 128, 387, 519, 708
BccI CCATC 3 cut(s) 443, 506, 565
BceAI ACGGC 2 cut(s) 113, 669
BciT130I CCWGG 1 cut(s) 56
BclI TGATCA 1 cut(s) 385
BcnI CCSGG 2 cut(s) 43, 350
BcoDI GTCTC 2 cut(s) 85, 190
BfmI CTRYAG 1 cut(s) 401
BfoI RGCGCY 1 cut(s) 115
BisI GCNGC 6 cut(s) 122, 142, 401, 533, 566, 722
BlsI GCNGC 6 cut(s) 123, 143, 402, 534, 567, 723
Bme1390I CCNGG 3 cut(s) 43, 56, 350
BmgT120I GGNCC 1 cut(s) 44
BmiI GGNNCC 2 cut(s) 428, 657
BmrFI CCNGG 3 cut(s) 43, 56, 350
BmrI ACTGGG 1 cut(s) 175
BmsI GCATC 3 cut(s) 432, 454, 682
BmuI ACTGGG 1 cut(s) 175
BplI GAGNNNNNCTC 4 cut(s) 521, 553, 799, 831
Bpu10I CCTNAGC 1 cut(s) 816
Bpu14I TTCGAA 1 cut(s) 93
BpuMI CCSGG 2 cut(s) 43, 350
BsaJI CCNNGG 1 cut(s) 338
Bsc4I CCNNNNNNNGG 2 cut(s) 115, 704
Bse118I RCCGGY 2 cut(s) 108, 137
Bse1I ACTGG 1 cut(s) 170
BseBI CCWGG 1 cut(s) 56
BseDI CCNNGG 1 cut(s) 338
BseGI GGATG 1 cut(s) 576
BseLI CCNNNNNNNGG 2 cut(s) 115, 704
BseMII CTCAG 1 cut(s) 830
BseNI ACTGG 1 cut(s) 170
BseRI GAGGAG 2 cut(s) 215, 223
BseXI GCAGC 5 cut(s) 108, 128, 387, 519, 708
BsgI GTGCAG 1 cut(s) 518
BshFI GGCC 4 cut(s) 41, 46, 59, 337
BshNI GGYRCC 1 cut(s) 655
BsiHKAI GWGCWC 1 cut(s) 72
BsiSI CCGG 4 cut(s) 42, 109, 138, 350
BslI CCNNNNNNNGG 2 cut(s) 115, 704
BsmAI GTCTC 2 cut(s) 85, 190
BsmBI CGTCTC 2 cut(s) 85, 190
BsmI GAATGC 2 cut(s) 579, 769
BsnI GGCC 4 cut(s) 41, 46, 59, 337
Bsp119I TTCGAA 1 cut(s) 93
Bsp1286I GDGCHC 1 cut(s) 72
Bsp143I GATC 3 cut(s) 385, 426, 781
Bsp19I CCATGG 1 cut(s) 338
BspACI CCGC 7 cut(s) 175, 246, 314, 369, 420, 565, 568
BspANI GGCC 4 cut(s) 41, 46, 59, 337
BspCNI CTCAG 1 cut(s) 829
BspHI TCATGA 1 cut(s) 835
BspLI GGNNCC 2 cut(s) 428, 657
BspMAI CTGCAG 1 cut(s) 405
BspPI GGATC 2 cut(s) 421, 434
BspQI GCTCTTC 1 cut(s) 689
BspT104I TTCGAA 1 cut(s) 93
BspT107I GGYRCC 1 cut(s) 655
BsrFI RCCGGY 2 cut(s) 108, 137
BsrI ACTGG 1 cut(s) 170
BssAI RCCGGY 2 cut(s) 108, 137
BssECI CCNNGG 1 cut(s) 338
BssMI GATC 3 cut(s) 385, 426, 781
BssT1I CCWWGG 1 cut(s) 338
Bst2UI CCWGG 1 cut(s) 56
Bst6I CTCTTC 4 cut(s) 107, 206, 689, 791
BstBI TTCGAA 1 cut(s) 93
BstC8I GCNNGC 5 cut(s) 126, 139, 312, 577, 822
BstDEI CTNAG 1 cut(s) 816
BstDSI CCRYGG 1 cut(s) 338
BstEII GGTNACC 1 cut(s) 631
BstF5I GGATG 1 cut(s) 576
BstH2I RGCGCY 1 cut(s) 115
BstHHI GCGC 2 cut(s) 114, 733
BstKTI GATC 3 cut(s) 388, 429, 784
BstMAI GTCTC 2 cut(s) 85, 190
BstMBI GATC 3 cut(s) 385, 426, 781
BstMWI GCNNNNNNNGC 6 cut(s) 118, 134, 350, 366, 653, 730
BstNI CCWGG 1 cut(s) 56
BstNSI RCATGY 3 cut(s) 314, 554, 824
BstPI GGTNACC 1 cut(s) 631
BstSCI CCNGG 3 cut(s) 41, 54, 348
BstSFI CTRYAG 1 cut(s) 401
BstV1I GCAGC 5 cut(s) 108, 128, 387, 519, 708
BstX2I RGATCY 1 cut(s) 426
BstYI RGATCY 1 cut(s) 426
BsuRI GGCC 4 cut(s) 41, 46, 59, 337
BtgI CCRYGG 1 cut(s) 338
BtgZI GCGATG 1 cut(s) 705
BtsCI GGATG 1 cut(s) 576
BtsIMutI CAGTG 2 cut(s) 258, 395
Cac8I GCNNGC 5 cut(s) 126, 139, 312, 577, 822
CciI TCATGA 1 cut(s) 835
CfoI GCGC 2 cut(s) 114, 733
Cfr10I RCCGGY 2 cut(s) 108, 137
Cfr13I GGNCC 1 cut(s) 44
CseI GACGC 1 cut(s) 596
Csp6I GTAC 1 cut(s) 330
CviAII CATG 7 cut(s) 311, 339, 366, 551, 791, 821, 836
CviQI GTAC 1 cut(s) 330
DdeI CTNAG 1 cut(s) 816
DpnI GATC 3 cut(s) 387, 428, 783
DpnII GATC 3 cut(s) 385, 426, 781
EaeI YGGCCR 2 cut(s) 57, 335
Eam1104I CTCTTC 4 cut(s) 107, 206, 689, 791
EarI CTCTTC 4 cut(s) 107, 206, 689, 791
EciI GGCGGA 1 cut(s) 583
Eco130I CCWWGG 1 cut(s) 338
Eco57I CTGAAG 1 cut(s) 234
Eco91I GGTNACC 1 cut(s) 631
EcoO65I GGTNACC 1 cut(s) 631
EcoRII CCWGG 1 cut(s) 54
EcoT14I CCWWGG 1 cut(s) 338
EcoT22I ATGCAT 1 cut(s) 447
ErhI CCWWGG 1 cut(s) 338
Esp3I CGTCTC 2 cut(s) 85, 190
FaeI CATG 7 cut(s) 314, 342, 369, 554, 794, 824, 839
FatI CATG 7 cut(s) 310, 338, 365, 550, 790, 820, 835
FauNDI CATATG 1 cut(s) 382
FbaI TGATCA 1 cut(s) 385
Fnu4HI GCNGC 6 cut(s) 122, 142, 401, 533, 566, 722
FokI GGATG 1 cut(s) 583
Fsp4HI GCNGC 6 cut(s) 122, 142, 401, 533, 566, 722
GlaI GCGC 2 cut(s) 113, 732
GluI GCNGC 6 cut(s) 122, 142, 401, 533, 566, 722
HaeII RGCGCY 1 cut(s) 115
HaeIII GGCC 4 cut(s) 41, 46, 59, 337
HapII CCGG 4 cut(s) 42, 109, 138, 350
HgaI GACGC 1 cut(s) 596
HhaI GCGC 2 cut(s) 114, 733
Hin1II CATG 7 cut(s) 314, 342, 369, 554, 794, 824, 839
Hin6I GCGC 2 cut(s) 112, 731
HinP1I GCGC 2 cut(s) 112, 731
HincII GTYRAC 1 cut(s) 208
HindII GTYRAC 1 cut(s) 208
HinfI GANTC 1 cut(s) 811
HpaII CCGG 4 cut(s) 42, 109, 138, 350
HphI GGTGA 1 cut(s) 487
Hpy166II GTNNAC 1 cut(s) 208
Hpy188I TCNGA 6 cut(s) 27, 118, 489, 707, 749, 762
Hpy188III TCNNGA 4 cut(s) 439, 584, 799, 836
Hpy8I GTNNAC 1 cut(s) 208
HpyAV CCTTC 2 cut(s) 441, 646
HpyCH4IV ACGT 1 cut(s) 328
HpyCH4V TGCA 5 cut(s) 380, 403, 445, 535, 550
HpyF10VI GCNNNNNNNGC 6 cut(s) 118, 134, 350, 366, 653, 730
HpyF3I CTNAG 1 cut(s) 816
HpySE526I ACGT 1 cut(s) 328
Hsp92II CATG 7 cut(s) 314, 342, 369, 554, 794, 824, 839
HspAI GCGC 2 cut(s) 112, 731
KroI GCCGGC 1 cut(s) 137
KroNI GCCGGC 1 cut(s) 139
Ksp22I TGATCA 1 cut(s) 385
Kzo9I GATC 3 cut(s) 385, 426, 781
LguI GCTCTTC 1 cut(s) 689
LmnI GCTCC 3 cut(s) 118, 228, 700
Lsp1109I GCAGC 5 cut(s) 108, 128, 387, 519, 708
LweI GCATC 3 cut(s) 432, 454, 682
MaeII ACGT 1 cut(s) 328
MaeIII GTNAC 5 cut(s) 191, 371, 493, 584, 631
MalI GATC 3 cut(s) 387, 428, 783
MboI GATC 3 cut(s) 385, 426, 781
MboII GAAGA 5 cut(s) 43, 94, 193, 676, 778
MflI RGATCY 1 cut(s) 426
MhlI GDGCHC 1 cut(s) 72
MlsI TGGCCA 2 cut(s) 59, 337
MluCI AATT 6 cut(s) 155, 462, 502, 600, 625, 701
MluNI TGGCCA 2 cut(s) 59, 337
MmeI TCCRAC 3 cut(s) 277, 304, 404
Mox20I TGGCCA 2 cut(s) 59, 337
Mph1103I ATGCAT 1 cut(s) 447
MroNI GCCGGC 1 cut(s) 137
MscI TGGCCA 2 cut(s) 59, 337
MseI TTAA 2 cut(s) 270, 624
MslI CAYNNNNRTG 3 cut(s) 65, 440, 825
Msp20I TGGCCA 2 cut(s) 59, 337
MspI CCGG 4 cut(s) 42, 109, 138, 350
MspR9I CCNGG 3 cut(s) 43, 56, 350
Mva1269I GAATGC 2 cut(s) 579, 769
MvaI CCWGG 1 cut(s) 56
MwoI GCNNNNNNNGC 6 cut(s) 118, 134, 350, 366, 653, 730
NaeI GCCGGC 1 cut(s) 139
NciI CCSGG 2 cut(s) 43, 350
NcoI CCATGG 1 cut(s) 338
NdeI CATATG 1 cut(s) 382
NdeII GATC 3 cut(s) 385, 426, 781
NgoMIV GCCGGC 1 cut(s) 137
NlaIII CATG 7 cut(s) 314, 342, 369, 554, 794, 824, 839
NlaIV GGNNCC 2 cut(s) 428, 657
NmuCI GTSAC 3 cut(s) 191, 493, 584
NsiI ATGCAT 1 cut(s) 447
NspI RCATGY 3 cut(s) 314, 554, 824
NspV TTCGAA 1 cut(s) 93
OliI CACNNNNGTG 1 cut(s) 65
PaeI GCATGC 2 cut(s) 314, 824
PagI TCATGA 1 cut(s) 835
PciSI GCTCTTC 1 cut(s) 689
PcsI WCGNNNNNNNCGW 1 cut(s) 23
PctI GAATGC 2 cut(s) 579, 769
PdiI GCCGGC 1 cut(s) 139
PfeI GAWTC 1 cut(s) 811
PkrI GCNGC 6 cut(s) 123, 143, 402, 534, 567, 723
Psp6I CCWGG 1 cut(s) 54
PspEI GGTNACC 1 cut(s) 631
PspGI CCWGG 1 cut(s) 54
PspN4I GGNNCC 2 cut(s) 428, 657
PspPI GGNCC 1 cut(s) 44
PstI CTGCAG 1 cut(s) 405
PsuI RGATCY 1 cut(s) 426
RsaI GTAC 1 cut(s) 331
RsaNI GTAC 1 cut(s) 330
RseI CAYNNNNRTG 3 cut(s) 65, 440, 825
SapI GCTCTTC 1 cut(s) 689
SaqAI TTAA 2 cut(s) 270, 624
SatI GCNGC 6 cut(s) 122, 142, 401, 533, 566, 722
Sau3AI GATC 3 cut(s) 385, 426, 781
Sau96I GGNCC 1 cut(s) 44
ScrFI CCNGG 3 cut(s) 43, 56, 350
SduI GDGCHC 1 cut(s) 72
SfaNI GCATC 3 cut(s) 432, 454, 682
SfcI CTRYAG 1 cut(s) 401
SfuI TTCGAA 1 cut(s) 93
SgrAI CRCCGGYG 1 cut(s) 108
SmiMI CAYNNNNRTG 3 cut(s) 65, 440, 825
SphI GCATGC 2 cut(s) 314, 824
Sse9I AATT 6 cut(s) 155, 462, 502, 600, 625, 701
SsiI CCGC 7 cut(s) 175, 246, 314, 369, 420, 565, 568
StyD4I CCNGG 3 cut(s) 41, 54, 348
StyI CCWWGG 1 cut(s) 338
TaiI ACGT 1 cut(s) 331
TaqI TCGA 1 cut(s) 93
TasI AATT 6 cut(s) 155, 462, 502, 600, 625, 701
TauI GCSGC 1 cut(s) 568
TfiI GAWTC 1 cut(s) 811
Tru1I TTAA 2 cut(s) 270, 624
Tru9I TTAA 2 cut(s) 270, 624
TscAI CASTG 2 cut(s) 258, 395
TseFI GTSAC 3 cut(s) 191, 493, 584
TseI GCWGC 5 cut(s) 121, 141, 400, 532, 721
Tsp45I GTSAC 3 cut(s) 191, 493, 584
TspDTI ATGAA 2 cut(s) 250, 767
TspGWI ACGGA 2 cut(s) 73, 205
TspRI CASTG 2 cut(s) 258, 395
XapI RAATTY 1 cut(s) 701
XceI RCATGY 3 cut(s) 314, 554, 824
Zsp2I ATGCAT 1 cut(s) 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.