Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0015200

Physical Location & Seq

Genomic Coordinates Reverse (-)

19831740 .. 19833281

Gene Structure 1542 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1167600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1542 bp

ATGACCCAGTTGCTGTTGAACTCAGTTATGGCTATGGCCATTGATGGGCATGATGACCCTGATCTTGATTTATCCGGCTGCAGCCCCACAACCACAAGCACCAACACCGTTTCCACCGATCACTGGTACGCCTGGTCTCCGCTGGTCGACTGGGAAGCGCTGGCACCGATAGACCAGCTCGCCGGAGACGACTTTCATGGGCTCATTGAGTCGATGGTGGAAGACCAAGATGGCAGCACTGAGCTGAACTCGTCGTCGGCCCAGGACGAGCCTCTAGAGTTAGAAGCGTCAAACGATACGACGACGTTGGGAGAAATGATGACGTTGACGGATGAAGACGGCTGCGCTAACGGTGAGGACTTGAAGGGGTTGAGGCTGGTCCATTTATTGATTGCGGCGGCGGAGGCGCTGACGGGCGCATACAAGAGCCGAGATCTGGCTCGGGTGATATTGGTTCGGCTCAAGGAGTTGGTCTCTCCAACTGACGGTTCTAACATGGAGAGGCTGGCGGCGTATTATACCGAGGCCCTCCAGGGTTTGCTAGAAGGCACCGGGGGTTTGCTTGGTAAGCATTTGATTGGGAGTGGGGCGCACCGCAATCACGGCCACCATCCAATGGACGTGACCGTAGCGTTTCAACTGCTCCAGGACATGTCGCCTTACGTTAAATTTGGGCACTTCACAGCCAACCAGGCGATTTTAGAGGCCGTGGTCCATGAGAGGCGGATCCACGTCGTAGATTATGACATCATGGAGGGTATCCAATGGGCTTCCTTGATGCAGGCCTTGGTTTCCAGGAAGGAGGGACCACCTACCCCACTTCTTAGGATCACCGCCTTGTCGAGGGGCGGCAGCAGAAGGCAGTCGATTGGGACCGTCCAAGAGACCGGCCGTCGTTTGACTGAATTTGCCGCGTCAATTGGTCAGCCATTTTCTTTTCACCAATGTAGGTTGGGTTCTGATGAGACATTTCAACCGTCCGCCTTGAAGTTGATAAAAGGGGAGACCTTGGTGATCAATTGTATGCTAAACCTCCCACATTTTAGTTACCGGTCTCCAGATTCAATTGCTTCGTTTTTATCCGGAGCCAAGACTCTTAGCCCGAGACTAGTCACTTTGGTCGAGGAAGATGTACGGTCCACATGGGATGGAGGCTTCGTGGCCCGCTTCATGGACTCATTGTACCATTACTCGGCAGTATATGACTCACTCGAGGCCGGCTTCCCAATGCAAAGCCGGGCTAGAGCTCTAGTGGAGAGAGTATTCTTGGGGCCTCGAATAGTAGGGTCATTGGCTCATATCTACCGCGCCCATGGAGAAGTTGGCCACTCTTGGAGGGAGAGGTTGGGTGCGGTAGGGTTTAAGCCTATTCCAATAAGCTTTGCCAATCATTGTCAAGCAAAACTTTTGTTGGGTCTTTTTAATGACGGTTATAGGGTGGAGGAGGTGACCAATCATCGGCTGGTTTTAGGTTGGAAGTCTCGTTGTTTACTCTCAGCTTCCATTTGGACGTCACCCTTAGAATCTGATGTTGTCAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0005783 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0012505 GO:0016020 GO:0019219 GO:0019222 GO:0031090 GO:0031323 GO:0031326 GO:0031965 GO:0031967 GO:0031975 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GRAS

Protein Analysis

514

Amino Acids

56.34

Weight (kDa)

5.36

Isoelectric Point (pI)

34.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GRAS	PF03514	126 - 504	8.4e-102	GRAS domain family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015200)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G08250
fragaria_vesca	FvH4_2g26540
malus_domestica	MD08G1167600.v1.1 MD15G1353200.v1.1
prunus_persica	Prupe.1G501500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr6g0295411
rosa_laevigata	RLG00000011806
rosa_multiflora	Rmu_co8465007.1_g000001 Rmu_sc0003058.1_g000004
rosa_roxburghii	Rroxscaffold_7G00172520
rosa_rugosa	Rorug06G0250400
rosa_samantha	Rh6AG363800 Rh6BG372000 Rh6CG378400 Rh6DG364800
rosa_wichuraiana	Rw6G031760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1514
AccB1I	GGYRCC	2 cut(s)	163, 548
AccB7I	CCANNNNNTGG	1 cut(s)	616
AccI	GTMKAC	1 cut(s)	147
AccII	CGCG	2 cut(s)	914, 1308
AccIII	TCCGGA	1 cut(s)	1082
AclWI	GGATC	3 cut(s)	721, 734, 836
AcoI	YGGCCR	4 cut(s)	36, 604, 889, 1324
AcsI	RAATTY	2 cut(s)	668, 905
AcyI	GRCGYC	1 cut(s)	1511
AfaI	GTAC	3 cut(s)	128, 1134, 1184
AfeI	AGCGCT	1 cut(s)	159
AfiI	CCNNNNNNNGG	9 cut(s)	45, 123, 436, 485, 616, 843, 1171, 1192, 1458
AflIII	ACRYGT	1 cut(s)	651
AgeI	ACCGGT	1 cut(s)	1050
AgsI	TTSAA	6 cut(s)	19, 364, 638, 974, 988, 1065
AhdI	GACNNNNNGTC	1 cut(s)	891
AhlI	ACTAGT	1 cut(s)	1108
AjiI	CACGTC	2 cut(s)	622, 733
AjnI	CCWGG	6 cut(s)	131, 261, 531, 645, 690, 794
AjuI	GAANNNNNNNTTGG	2 cut(s)	472, 504
AluBI	AGCT	5 cut(s)	178, 244, 1247, 1380, 1499
AluI	AGCT	5 cut(s)	178, 244, 1247, 1380, 1499
Alw21I	GWGCWC	1 cut(s)	1249
Alw26I	GTCTC	9 cut(s)	141, 180, 478, 878, 959, 998, 1059, 1099, 1485
AlwI	GGATC	3 cut(s)	721, 734, 836
AlwNI	CAGNNNCTG	1 cut(s)	13
Ama87I	CYCGRG	3 cut(s)	441, 1102, 1211
Aor13HI	TCCGGA	1 cut(s)	1082
Aor51HI	AGCGCT	1 cut(s)	159
ApeKI	GCWGC	5 cut(s)	78, 81, 234, 342, 852
ApoI	RAATTY	2 cut(s)	668, 905
AsiGI	ACCGGT	1 cut(s)	1050
AspLEI	GCGC	6 cut(s)	160, 347, 409, 419, 592, 1310
AspS9I	GGNCC	9 cut(s)	259, 379, 526, 712, 806, 873, 1137, 1162, 1271
AsuC2I	CCSGG	2 cut(s)	553, 1238
AsuHPI	GGTGA	7 cut(s)	365, 457, 823, 932, 1024, 1459, 1506
AvaI	CYCGRG	3 cut(s)	441, 1102, 1211
AvaII	GGWCC	5 cut(s)	379, 712, 806, 873, 1137
BaeGI	GKGCMC	1 cut(s)	678
BalI	TGGCCA	2 cut(s)	38, 1326
BamHI	GGATCC	1 cut(s)	726
BanI	GGYRCC	2 cut(s)	163, 548
BanII	GRGCYC	2 cut(s)	204, 1249
BbsI	GAAGAC	2 cut(s)	228, 342
Bbv12I	GWGCWC	1 cut(s)	1249
BbvI	GCAGC	5 cut(s)	65, 93, 246, 329, 864
BccI	CCATC	5 cut(s)	38, 208, 224, 618, 1142
BceAI	ACGGC	4 cut(s)	355, 619, 692, 876
BciT130I	CCWGG	6 cut(s)	133, 263, 533, 647, 692, 796
BciVI	GTATCC	1 cut(s)	770
BclI	TGATCA	1 cut(s)	1014
BcnI	CCSGG	2 cut(s)	553, 1238
BcoDI	GTCTC	9 cut(s)	141, 180, 478, 878, 959, 998, 1059, 1099, 1485
BcuI	ACTAGT	1 cut(s)	1108
BfaI	CTAG	5 cut(s)	275, 542, 1109, 1242, 1250
BfmI	CTRYAG	1 cut(s)	79
BfoI	RGCGCY	2 cut(s)	161, 410
BfuI	GTATCC	1 cut(s)	770
BglI	GCCNNNNNGGC	1 cut(s)	692
BglII	AGATCT	1 cut(s)	433
Bme1390I	CCNGG	8 cut(s)	133, 263, 533, 553, 647, 692, 796, 1238
Bme18I	GGWCC	5 cut(s)	379, 712, 806, 873, 1137
BmeRI	GACNNNNNGTC	1 cut(s)	891
BmeT110I	CYCGRG	3 cut(s)	441, 1102, 1211
BmgBI	CACGTC	2 cut(s)	622, 733
BmgT120I	GGNCC	9 cut(s)	259, 379, 526, 712, 806, 873, 1137, 1162, 1271
BmiI	GGNNCC	7 cut(s)	165, 550, 728, 807, 874, 1087, 1272
BmrFI	CCNGG	8 cut(s)	133, 263, 533, 553, 647, 692, 796, 1238
BmrI	ACTGGG	1 cut(s)	160
BmsI	GCATC	1 cut(s)	768
BmuI	ACTGGG	1 cut(s)	160
BpiI	GAAGAC	2 cut(s)	228, 342
BplI	GAGNNNNNCTC	4 cut(s)	233, 265, 458, 490
BpmI	CTGGAG	3 cut(s)	515, 629, 1041
BpuEI	CTTGAG	1 cut(s)	446
BpuMI	CCSGG	2 cut(s)	553, 1238
BsaHI	GRCGYC	1 cut(s)	1511
BsaI	GGTCTC	5 cut(s)	141, 478, 878, 998, 1059
BsaJI	CCNNGG	8 cut(s)	261, 522, 532, 552, 708, 786, 1008, 1312
BsaWI	WCCGGW	2 cut(s)	1050, 1082
BsaXI	ACNNNNNCTCC	2 cut(s)	1143, 1173
Bsc4I	CCNNNNNNNGG	9 cut(s)	45, 123, 436, 485, 616, 843, 1171, 1192, 1458
Bse118I	RCCGGY	3 cut(s)	887, 1050, 1217
Bse1I	ACTGG	3 cut(s)	7, 128, 155
BseAI	TCCGGA	1 cut(s)	1082
BseBI	CCWGG	6 cut(s)	133, 263, 533, 647, 692, 796
BseDI	CCNNGG	8 cut(s)	261, 522, 532, 552, 708, 786, 1008, 1312
BseGI	GGATG	3 cut(s)	337, 610, 1153
BseLI	CCNNNNNNNGG	9 cut(s)	45, 123, 436, 485, 616, 843, 1171, 1192, 1458
BseMII	CTCAG	3 cut(s)	36, 231, 1509
BseNI	ACTGG	3 cut(s)	7, 128, 155
BseRI	GAGGAG	1 cut(s)	1457
BseSI	GKGCMC	1 cut(s)	678
BseX3I	CGGCCG	1 cut(s)	889
BseXI	GCAGC	5 cut(s)	65, 93, 246, 329, 864
Bsh1236I	CGCG	2 cut(s)	914, 1308
Bsh1285I	CGRYCG	1 cut(s)	892
BshNI	GGYRCC	2 cut(s)	163, 548
BshTI	ACCGGT	1 cut(s)	1050
BsiEI	CGRYCG	1 cut(s)	892
BsiHKAI	GWGCWC	1 cut(s)	1249
BsiHKCI	CYCGRG	3 cut(s)	441, 1102, 1211
BsiSI	CCGG	8 cut(s)	75, 183, 552, 888, 1051, 1083, 1218, 1237
BslFI	GGGAC	2 cut(s)	819, 886
BslI	CCNNNNNNNGG	9 cut(s)	45, 123, 436, 485, 616, 843, 1171, 1192, 1458
BsmAI	GTCTC	9 cut(s)	141, 180, 478, 878, 959, 998, 1059, 1099, 1485
BsmBI	CGTCTC	1 cut(s)	180
BsmFI	GGGAC	2 cut(s)	819, 886
Bso31I	GGTCTC	5 cut(s)	141, 478, 878, 998, 1059
BsoBI	CYCGRG	3 cut(s)	441, 1102, 1211
Bsp1286I	GDGCHC	3 cut(s)	204, 678, 1249
Bsp13I	TCCGGA	1 cut(s)	1082
Bsp143I	GATC	6 cut(s)	61, 118, 433, 726, 828, 1014
Bsp19I	CCATGG	1 cut(s)	1312
BspCNI	CTCAG	3 cut(s)	35, 232, 1508
BspEI	TCCGGA	1 cut(s)	1082
BspFNI	CGCG	2 cut(s)	914, 1308
BspLI	GGNNCC	7 cut(s)	165, 550, 728, 807, 874, 1087, 1272
BspMAI	CTGCAG	1 cut(s)	83
BspPI	GGATC	3 cut(s)	721, 734, 836
BspT107I	GGYRCC	2 cut(s)	163, 548
BspTNI	GGTCTC	5 cut(s)	141, 478, 878, 998, 1059
BsrFI	RCCGGY	3 cut(s)	887, 1050, 1217
BsrI	ACTGG	3 cut(s)	7, 128, 155
BssAI	RCCGGY	3 cut(s)	887, 1050, 1217
BssECI	CCNNGG	8 cut(s)	261, 522, 532, 552, 708, 786, 1008, 1312
BssMI	GATC	6 cut(s)	61, 118, 433, 726, 828, 1014
BssNI	GRCGYC	1 cut(s)	1511
BssT1I	CCWWGG	3 cut(s)	786, 1008, 1312
Bst2UI	CCWGG	6 cut(s)	133, 263, 533, 647, 692, 796
Bst4CI	ACNGT	8 cut(s)	109, 353, 488, 628, 877, 978, 1137, 1430
BstACI	GRCGYC	1 cut(s)	1511
BstC8I	GCNNGC	6 cut(s)	162, 180, 507, 783, 1165, 1219
BstDEI	CTNAG	6 cut(s)	22, 240, 824, 1097, 1495, 1519
BstDSI	CCRYGG	2 cut(s)	708, 1312
BstEII	GGTNACC	1 cut(s)	1447
BstENI	CCTNNNNNAGG	1 cut(s)	841
BstF5I	GGATG	3 cut(s)	337, 610, 1153
BstFNI	CGCG	2 cut(s)	914, 1308
BstH2I	RGCGCY	2 cut(s)	161, 410
BstHHI	GCGC	6 cut(s)	160, 347, 409, 419, 592, 1310
BstKTI	GATC	6 cut(s)	64, 121, 436, 729, 831, 1017
BstMAI	GTCTC	9 cut(s)	141, 180, 478, 878, 959, 998, 1059, 1099, 1485
BstMBI	GATC	6 cut(s)	61, 118, 433, 726, 828, 1014
BstMCI	CGRYCG	1 cut(s)	892
BstMWI	GCNNNNNNNGC	4 cut(s)	404, 568, 603, 692
BstNI	CCWGG	6 cut(s)	133, 263, 533, 647, 692, 796
BstNSI	RCATGY	1 cut(s)	655
BstPI	GGTNACC	1 cut(s)	1447
BstSCI	CCNGG	8 cut(s)	131, 261, 531, 551, 645, 690, 794, 1236
BstSFI	CTRYAG	1 cut(s)	79
BstSLI	GKGCMC	1 cut(s)	678
BstUI	CGCG	2 cut(s)	914, 1308
BstV1I	GCAGC	5 cut(s)	65, 93, 246, 329, 864
BstV2I	GAAGAC	2 cut(s)	228, 342
BstX2I	RGATCY	2 cut(s)	433, 726
BstYI	RGATCY	2 cut(s)	433, 726
BstZI	CGGCCG	1 cut(s)	889
BsuI	GTATCC	1 cut(s)	770
BtgI	CCRYGG	2 cut(s)	708, 1312
BtrI	CACGTC	2 cut(s)	622, 733
BtsCI	GGATG	3 cut(s)	337, 610, 1153
BtsIMutI	CAGTG	2 cut(s)	121, 237
Cac8I	GCNNGC	6 cut(s)	162, 180, 507, 783, 1165, 1219
CaiI	CAGNNNCTG	1 cut(s)	13
CfoI	GCGC	6 cut(s)	160, 347, 409, 419, 592, 1310
Cfr10I	RCCGGY	3 cut(s)	887, 1050, 1217
Cfr13I	GGNCC	9 cut(s)	259, 379, 526, 712, 806, 873, 1137, 1162, 1271
CseI	GACGC	2 cut(s)	276, 903
Csp6I	GTAC	3 cut(s)	127, 1133, 1183
CspAI	ACCGGT	1 cut(s)	1050
CviAII	CATG	9 cut(s)	50, 197, 496, 652, 716, 751, 1143, 1171, 1313
CviQI	GTAC	3 cut(s)	127, 1133, 1183
DdeI	CTNAG	6 cut(s)	22, 240, 824, 1097, 1495, 1519
DpnI	GATC	6 cut(s)	63, 120, 435, 728, 830, 1016
DpnII	GATC	6 cut(s)	61, 118, 433, 726, 828, 1014
DriI	GACNNNNNGTC	1 cut(s)	891
EaeI	YGGCCR	4 cut(s)	36, 604, 889, 1324
EagI	CGGCCG	1 cut(s)	889
Eam1105I	GACNNNNNGTC	1 cut(s)	891
EciI	GGCGGA	3 cut(s)	416, 739, 970
Ecl136II	GAGCTC	1 cut(s)	1247
EclXI	CGGCCG	1 cut(s)	889
Eco130I	CCWWGG	3 cut(s)	786, 1008, 1312
Eco147I	AGGCCT	1 cut(s)	785
Eco24I	GRGCYC	2 cut(s)	204, 1249
Eco31I	GGTCTC	5 cut(s)	141, 478, 878, 998, 1059
Eco47I	GGWCC	5 cut(s)	379, 712, 806, 873, 1137
Eco47III	AGCGCT	1 cut(s)	159
Eco52I	CGGCCG	1 cut(s)	889
Eco53kI	GAGCTC	1 cut(s)	1247
Eco88I	CYCGRG	3 cut(s)	441, 1102, 1211
Eco91I	GGTNACC	1 cut(s)	1447
EcoICRI	GAGCTC	1 cut(s)	1247
EcoNI	CCTNNNNNAGG	1 cut(s)	841
EcoO109I	RGGNCCY	2 cut(s)	526, 1271
EcoO65I	GGTNACC	1 cut(s)	1447
EcoRII	CCWGG	6 cut(s)	131, 261, 531, 645, 690, 794
EcoT14I	CCWWGG	3 cut(s)	786, 1008, 1312
EcoT38I	GRGCYC	2 cut(s)	204, 1249
ErhI	CCWWGG	3 cut(s)	786, 1008, 1312
Esp3I	CGTCTC	1 cut(s)	180
FaeI	CATG	9 cut(s)	53, 200, 499, 655, 719, 754, 1146, 1174, 1316
FalI	AAGNNNNNCTT	2 cut(s)	1389, 1421
FaqI	GGGAC	2 cut(s)	819, 886
FatI	CATG	9 cut(s)	49, 196, 495, 651, 715, 750, 1142, 1170, 1312
FauI	CCCGC	1 cut(s)	1172
FbaI	TGATCA	1 cut(s)	1014
FblI	GTMKAC	1 cut(s)	147
FokI	GGATG	3 cut(s)	344, 597, 1160
FriOI	GRGCYC	2 cut(s)	204, 1249
FspBI	CTAG	5 cut(s)	275, 542, 1109, 1242, 1250
GlaI	GCGC	6 cut(s)	159, 346, 408, 418, 591, 1309
GsuI	CTGGAG	3 cut(s)	515, 629, 1041
HaeII	RGCGCY	2 cut(s)	161, 410
HapII	CCGG	8 cut(s)	75, 183, 552, 888, 1051, 1083, 1218, 1237
HgaI	GACGC	2 cut(s)	276, 903
HhaI	GCGC	6 cut(s)	160, 347, 409, 419, 592, 1310
Hin1I	GRCGYC	1 cut(s)	1511
Hin1II	CATG	9 cut(s)	53, 200, 499, 655, 719, 754, 1146, 1174, 1316
Hin6I	GCGC	6 cut(s)	158, 345, 407, 417, 590, 1308
HinP1I	GCGC	6 cut(s)	158, 345, 407, 417, 590, 1308
HincII	GTYRAC	2 cut(s)	148, 327
HindII	GTYRAC	2 cut(s)	148, 327
HindIII	AAGCTT	1 cut(s)	1378
HinfI	GANTC	6 cut(s)	209, 1061, 1093, 1175, 1205, 1523
HpaII	CCGG	8 cut(s)	75, 183, 552, 888, 1051, 1083, 1218, 1237
HphI	GGTGA	7 cut(s)	365, 457, 823, 932, 1024, 1459, 1506
Hpy166II	GTNNAC	4 cut(s)	148, 327, 1140, 1490
Hpy188I	TCNGA	2 cut(s)	961, 1528
Hpy188III	TCNNGA	4 cut(s)	65, 275, 1058, 1083
Hpy8I	GTNNAC	4 cut(s)	148, 327, 1140, 1490
Hpy99I	CGWCG	6 cut(s)	256, 259, 304, 307, 737, 897
HpyAV	CCTTC	4 cut(s)	358, 539, 793, 852
HpyCH4III	ACNGT	8 cut(s)	109, 353, 488, 628, 877, 978, 1137, 1430
HpyCH4IV	ACGT	6 cut(s)	305, 323, 621, 663, 732, 1511
HpyCH4V	TGCA	3 cut(s)	81, 781, 1231
HpyF10VI	GCNNNNNNNGC	4 cut(s)	404, 568, 603, 692
HpyF3I	CTNAG	6 cut(s)	22, 240, 824, 1097, 1495, 1519
HpySE526I	ACGT	6 cut(s)	305, 323, 621, 663, 732, 1511
Hsp92I	GRCGYC	1 cut(s)	1511
Hsp92II	CATG	9 cut(s)	53, 200, 499, 655, 719, 754, 1146, 1174, 1316
HspAI	GCGC	6 cut(s)	158, 345, 407, 417, 590, 1308
Kpn2I	TCCGGA	1 cut(s)	1082
KroI	GCCGGC	1 cut(s)	1217
KroNI	GCCGGC	1 cut(s)	1219
Ksp22I	TGATCA	1 cut(s)	1014
Kzo9I	GATC	6 cut(s)	61, 118, 433, 726, 828, 1014
LmnI	GCTCC	2 cut(s)	648, 1085
Lsp1109I	GCAGC	5 cut(s)	65, 93, 246, 329, 864
LweI	GCATC	1 cut(s)	768
MaeI	CTAG	5 cut(s)	275, 542, 1109, 1242, 1250
MaeII	ACGT	6 cut(s)	305, 323, 621, 663, 732, 1511
MaeIII	GTNAC	5 cut(s)	622, 1046, 1111, 1447, 1512
MalI	GATC	6 cut(s)	63, 120, 435, 728, 830, 1016
MboI	GATC	6 cut(s)	61, 118, 433, 726, 828, 1014
MboII	GAAGA	3 cut(s)	233, 347, 1139
MfeI	CAATTG	3 cut(s)	918, 1018, 1065
MflI	RGATCY	2 cut(s)	433, 726
MhlI	GDGCHC	3 cut(s)	204, 678, 1249
MlsI	TGGCCA	2 cut(s)	38, 1326
MluCI	AATT	6 cut(s)	668, 905, 918, 1018, 1065, 1537
MluNI	TGGCCA	2 cut(s)	38, 1326
MlyI	GAGTC	4 cut(s)	218, 1087, 1169, 1199
MmeI	TCCRAC	2 cut(s)	503, 1454
Mox20I	TGGCCA	2 cut(s)	38, 1326
MroI	TCCGGA	1 cut(s)	1082
MroNI	GCCGGC	1 cut(s)	1217
MscI	TGGCCA	2 cut(s)	38, 1326
MseI	TTAA	3 cut(s)	666, 1362, 1422
Msp20I	TGGCCA	2 cut(s)	38, 1326
MspA1I	CMGCKG	1 cut(s)	142
MspI	CCGG	8 cut(s)	75, 183, 552, 888, 1051, 1083, 1218, 1237
MspR9I	CCNGG	8 cut(s)	133, 263, 533, 553, 647, 692, 796, 1238
MunI	CAATTG	3 cut(s)	918, 1018, 1065
MvaI	CCWGG	6 cut(s)	133, 263, 533, 647, 692, 796
MvnI	CGCG	2 cut(s)	914, 1308
MwoI	GCNNNNNNNGC	4 cut(s)	404, 568, 603, 692
NaeI	GCCGGC	1 cut(s)	1219
NciI	CCSGG	2 cut(s)	553, 1238
NcoI	CCATGG	1 cut(s)	1312
NdeII	GATC	6 cut(s)	61, 118, 433, 726, 828, 1014
NgoMIV	GCCGGC	1 cut(s)	1217
NlaIII	CATG	9 cut(s)	53, 200, 499, 655, 719, 754, 1146, 1174, 1316
NlaIV	GGNNCC	7 cut(s)	165, 550, 728, 807, 874, 1087, 1272
NmeAIII	GCCGAG	2 cut(s)	455, 1172
NmuCI	GTSAC	4 cut(s)	622, 1111, 1447, 1512
NspI	RCATGY	1 cut(s)	655
PaeR7I	CTCGAG	1 cut(s)	1211
PceI	AGGCCT	1 cut(s)	785
PciI	ACATGT	1 cut(s)	651
PcsI	WCGNNNNNNNCGW	1 cut(s)	186
PdiI	GCCGGC	1 cut(s)	1219
PfeI	GAWTC	2 cut(s)	1061, 1523
PflMI	CCANNNNNTGG	1 cut(s)	616
PfoI	TCCNGGA	2 cut(s)	645, 794
PinAI	ACCGGT	1 cut(s)	1050
PleI	GAGTC	4 cut(s)	217, 1087, 1169, 1199
PpsI	GAGTC	4 cut(s)	217, 1087, 1169, 1199
PscI	ACATGT	1 cut(s)	651
Psp124BI	GAGCTC	1 cut(s)	1249
Psp6I	CCWGG	6 cut(s)	131, 261, 531, 645, 690, 794
PspEI	GGTNACC	1 cut(s)	1447
PspGI	CCWGG	6 cut(s)	131, 261, 531, 645, 690, 794
PspN4I	GGNNCC	7 cut(s)	165, 550, 728, 807, 874, 1087, 1272
PspPI	GGNCC	9 cut(s)	259, 379, 526, 712, 806, 873, 1137, 1162, 1271
PspXI	VCTCGAGB	1 cut(s)	1211
PsrI	GAACNNNNNNTAC	2 cut(s)	940, 972
PstI	CTGCAG	1 cut(s)	83
PstNI	CAGNNNCTG	1 cut(s)	13
PsuI	RGATCY	2 cut(s)	433, 726
RsaI	GTAC	3 cut(s)	128, 1134, 1184
RsaNI	GTAC	3 cut(s)	127, 1133, 1183
SacI	GAGCTC	1 cut(s)	1249
SalI	GTCGAC	1 cut(s)	146
SaqAI	TTAA	3 cut(s)	666, 1362, 1422
Sau3AI	GATC	6 cut(s)	61, 118, 433, 726, 828, 1014
Sau96I	GGNCC	9 cut(s)	259, 379, 526, 712, 806, 873, 1137, 1162, 1271
SchI	GAGTC	4 cut(s)	218, 1087, 1169, 1199
ScrFI	CCNGG	8 cut(s)	133, 263, 533, 553, 647, 692, 796, 1238
SduI	GDGCHC	3 cut(s)	204, 678, 1249
SfaNI	GCATC	1 cut(s)	768
SfcI	CTRYAG	1 cut(s)	79
Sfr274I	CTCGAG	1 cut(s)	1211
SinI	GGWCC	5 cut(s)	379, 712, 806, 873, 1137
SlaI	CTCGAG	1 cut(s)	1211
SmlI	CTYRAG	2 cut(s)	461, 1211
SmoI	CTYRAG	2 cut(s)	461, 1211
SpeI	ACTAGT	1 cut(s)	1108
Sse9I	AATT	6 cut(s)	668, 905, 918, 1018, 1065, 1537
SseBI	AGGCCT	1 cut(s)	785
SspMI	CTAG	5 cut(s)	275, 542, 1109, 1242, 1250
SstI	GAGCTC	1 cut(s)	1249
StuI	AGGCCT	1 cut(s)	785
StyD4I	CCNGG	8 cut(s)	131, 261, 531, 551, 645, 690, 794, 1236
StyI	CCWWGG	3 cut(s)	786, 1008, 1312
TaaI	ACNGT	8 cut(s)	109, 353, 488, 628, 877, 978, 1137, 1430
TaiI	ACGT	6 cut(s)	308, 326, 624, 666, 735, 1514
TaqI	TCGA	7 cut(s)	147, 212, 842, 866, 1122, 1212, 1276
TasI	AATT	6 cut(s)	668, 905, 918, 1018, 1065, 1537
TauI	GCSGC	5 cut(s)	398, 401, 512, 852, 914
TfiI	GAWTC	2 cut(s)	1061, 1523
Tru1I	TTAA	3 cut(s)	666, 1362, 1422
Tru9I	TTAA	3 cut(s)	666, 1362, 1422
TscAI	CASTG	2 cut(s)	128, 244
TseFI	GTSAC	4 cut(s)	622, 1111, 1447, 1512
TseI	GCWGC	5 cut(s)	78, 81, 234, 342, 852
Tsp45I	GTSAC	4 cut(s)	622, 1111, 1447, 1512
TspDTI	ATGAA	3 cut(s)	185, 348, 1159
TspGWI	ACGGA	1 cut(s)	344
TspRI	CASTG	2 cut(s)	128, 244
Van91I	CCANNNNNTGG	1 cut(s)	616
VpaK11BI	GGWCC	5 cut(s)	379, 712, 806, 873, 1137
XagI	CCTNNNNNAGG	1 cut(s)	841
XapI	RAATTY	2 cut(s)	668, 905
XbaI	TCTAGA	1 cut(s)	274
XceI	RCATGY	1 cut(s)	655
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1319, 1459
XhoI	CTCGAG	1 cut(s)	1211
XmiI	GTMKAC	1 cut(s)	147
XspI	CTAG	5 cut(s)	275, 542, 1109, 1242, 1250
ZraI	GACGTC	1 cut(s)	1512

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.