MD08G1173800.v1.1
NAC Family

Inactive rhomboid protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0014538
Physical Location & Seq
Reverse (-)
21128936 .. 21132174
3239 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1173800.v1.1.491

Sequence Viewer

Length: 1173 bp
ATGGCCGCAGACCCATCAAAGCTTCAGACCCAGATCGACATCAAGCCTCCTCCGCTTCCATTCCACACTGAATCAGCTCCCGAAACTCTTCAAGAACCCAAGCTCTCCTTCTTCGCCTCCCGCTACCGTCGCACTCGCCGCCGTCGAGCCGACACATGGCTCATATCTCTCTTCGTGATCCTACACATCGTGGCCTTCACGGCGACTATGGTCGTCAACGACTGCTGGCACAAGTCTCGTGGCGACTGCGCCCTCAAAGCCTTTGGCCGGCTGTCGTTTCAGCCACTCTCCGAGAACCCGCTGCTTGGCCCTTCCGCTTCCACGCTTGATGAAATGGGAGCTCTTCGACGGACCTTTTTGACAGAGAATCGCCAAACATGGCGTCTTTTCACATTTCCATGTCTGCATGCCGGAGCCATCCACCTTGTGATCAACCTTTGCAGTGTCGTTTTTGTTGGAATTCACTTGGAGCAAGAGTTTGGTCCATTAATGGCAGGGTTAATCTATATACTCTCTGCTTTTGTTGGTACGTTGGTGGCTGCACTCTTTGTTGAGAAACACCCAGCAGTTGGTGCTTCTGGAGCTCTATATGGGTTACTTGGAGCTACACTTGCAGCGCTGATTCAGAACTGGGAAATGTACACCAATAAGTTCACAGCTCTAGTATCAGTTCTATTTGTCTCCATTGGGAATTTTCTCCTCGGCTTGCTACCTTATGTGGACAACTTTTCGAGCATTGGAGGCTTTATATCAGGGTTCCTACTTGGATCTGTGCTTTTATCCAGTCCTAAGGTCAGAGAACTGGCTCAAGAGAAAGGAGGCCTCCTTGATTATGATCTCAAAAGTTCTATCAAGTTGAGGATGAGGCTGAAGCTGGACAGACCAGTTTATAGGAGTTTGGCTTTTGTTCTCTTTGGTGTTATGCTGGCTGGATGTCTTGTTGCAGTTCTGCAAGGCATTGACCTGAATCAGTACTGCAGGTGGTGCCGGCATTTTGATTGTATCCCTTTCAAAGGATGGAGCTGCAGGGACATGGAAACTTTTTGTGAGACCATGGTGAGCAATGAACAGCTGACTTTGACCTGTATGAGCAATGGGAACTTCAGGGTTCTTCCATACACAAATGTCTCTCAAGATCGGGTGAATGACTTGTGCAGTGTGATATGCTCATAG

Protein Analysis

391

Amino Acids

43.46

Weight (kDa)

8.66

Isoelectric Point (pI)

40.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhomboid PF01694 124 - 261 2.2e-32 Rhomboid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014538)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G77860 AT1G77860
fragaria_vesca FvH4_5g38430
malus_domestica MD08G1173800.v1.1
prunus_persica Prupe.1G507800_v2.0.a1 Prupe.1G507800_v2.0.a1 Prupe.1G507800_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0240461
rosa_laevigata RLG00000000677
rosa_multiflora Rmu_sc0005327.1_g000015
rosa_roxburghii Rroxscaffold_3G00219970
rosa_rugosa Rorug07G0336300
rosa_samantha Rh7AG490600 Rh7BG463300 Rh7CG506800 Rh7DG474400
rosa_wichuraiana Rw7G041120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 969
Acc36I ACCTGC 1 cut(s) 969
AccB1I GGYRCC 1 cut(s) 984
AccB7I CCANNNNNTGG 1 cut(s) 569
AciI CCGC 6 cut(s) 6, 53, 121, 139, 299, 315
AclWI GGATC 2 cut(s) 172, 775
AcoI YGGCCR 2 cut(s) 3, 265
AcsI RAATTY 2 cut(s) 459, 691
AcuI CTGAAG 3 cut(s) 8, 890, 1087
AcyI GRCGYC 1 cut(s) 382
AdeI CACNNNGTG 2 cut(s) 190, 427
AfaI GTAC 3 cut(s) 529, 641, 974
AfeI AGCGCT 1 cut(s) 618
AfiI CCNNNNNNNGG 5 cut(s) 156, 267, 305, 569, 1013
AgsI TTSAA 2 cut(s) 92, 1012
AjuI GAANNNNNNNTTGG 2 cut(s) 92, 124
Alw21I GWGCWC 2 cut(s) 343, 586
Alw26I GTCTC 4 cut(s) 240, 685, 1043, 1132
AlwI GGATC 2 cut(s) 172, 775
Aor51HI AGCGCT 1 cut(s) 618
AoxI GGCC 5 cut(s) 3, 192, 265, 307, 820
ApeKI GCWGC 4 cut(s) 301, 539, 614, 1023
ApoI RAATTY 2 cut(s) 459, 691
ArsI GACNNNNNNTTYG 2 cut(s) 367, 399
AseI ATTAAT 1 cut(s) 488
Asp700I GAANNNNTTC 1 cut(s) 87
AspLEI GCGC 2 cut(s) 251, 619
AspS9I GGNCC 3 cut(s) 308, 351, 482
AsuHPI GGTGA 2 cut(s) 1069, 1153
AvaII GGWCC 2 cut(s) 351, 482
AxyI CCTNAGG 1 cut(s) 789
BaeI ACNNNNGTAYC 2 cut(s) 519, 552
BanI GGYRCC 1 cut(s) 984
BanII GRGCYC 2 cut(s) 343, 586
BauI CACGAG 1 cut(s) 237
Bbv12I GWGCWC 2 cut(s) 343, 586
BbvI GCAGC 4 cut(s) 288, 526, 626, 1010
BccI CCATC 3 cut(s) 22, 425, 1011
BceAI ACGGC 2 cut(s) 126, 216
BcgI CGANNNNNNTGC 2 cut(s) 218, 252
BciVI GTATCC 1 cut(s) 1013
BclI TGATCA 1 cut(s) 429
BcoDI GTCTC 4 cut(s) 240, 685, 1043, 1132
BfaI CTAG 1 cut(s) 662
BfmI CTRYAG 2 cut(s) 976, 1024
BfoI RGCGCY 1 cut(s) 620
BfuAI ACCTGC 1 cut(s) 969
BfuI GTATCC 1 cut(s) 1013
BglI GCCNNNNNGGC 1 cut(s) 200
BisI GCNGC 6 cut(s) 6, 139, 302, 540, 615, 1024
BlsI GCNGC 6 cut(s) 7, 140, 303, 541, 616, 1025
BmcAI AGTACT 1 cut(s) 974
Bme18I GGWCC 2 cut(s) 351, 482
BmgT120I GGNCC 3 cut(s) 308, 351, 482
BmiI GGNNCC 3 cut(s) 415, 758, 986
BmrI ACTGGG 1 cut(s) 640
BmuI ACTGGG 1 cut(s) 640
BoxI GACNNNNGTC 1 cut(s) 209
BpmI CTGGAG 1 cut(s) 600
BpuEI CTTGAG 2 cut(s) 792, 1116
BsaBI GATNNNNATC 2 cut(s) 38, 834
BsaHI GRCGYC 1 cut(s) 382
BsaI GGTCTC 1 cut(s) 1043
BsaJI CCNNGG 2 cut(s) 700, 1053
BsaXI ACNNNNNCTCC 2 cut(s) 461, 491
Bsc4I CCNNNNNNNGG 5 cut(s) 156, 267, 305, 569, 1013
Bse118I RCCGGY 2 cut(s) 267, 987
Bse1I ACTGG 4 cut(s) 635, 783, 807, 884
Bse21I CCTNAGG 1 cut(s) 789
Bse3DI GCAATG 2 cut(s) 1069, 1099
Bse8I GATNNNNATC 2 cut(s) 38, 834
BseDI CCNNGG 2 cut(s) 700, 1053
BseGI GGATG 4 cut(s) 417, 867, 938, 1022
BseJI GATNNNNATC 2 cut(s) 38, 834
BseLI CCNNNNNNNGG 5 cut(s) 156, 267, 305, 569, 1013
BseMI GCAATG 2 cut(s) 1069, 1099
BseNI ACTGG 4 cut(s) 635, 783, 807, 884
BseRI GAGGAG 2 cut(s) 39, 689
BseXI GCAGC 4 cut(s) 288, 526, 626, 1010
BseYI CCCAGC 1 cut(s) 562
BsgI GTGCAG 1 cut(s) 525
BshFI GGCC 5 cut(s) 5, 194, 267, 309, 822
BshNI GGYRCC 1 cut(s) 984
BsiHKAI GWGCWC 2 cut(s) 343, 586
BsiSI CCGG 3 cut(s) 268, 411, 988
BslFI GGGAC 1 cut(s) 1043
BslI CCNNNNNNNGG 5 cut(s) 156, 267, 305, 569, 1013
BsmAI GTCTC 4 cut(s) 240, 685, 1043, 1132
BsmFI GGGAC 1 cut(s) 1043
BsnI GGCC 5 cut(s) 5, 194, 267, 309, 822
Bso31I GGTCTC 1 cut(s) 1043
Bsp1286I GDGCHC 2 cut(s) 343, 586
Bsp1407I TGTACA 1 cut(s) 639
Bsp143I GATC 6 cut(s) 33, 177, 429, 767, 835, 1135
Bsp19I CCATGG 1 cut(s) 1053
BspACI CCGC 6 cut(s) 6, 53, 121, 139, 299, 315
BspANI GGCC 5 cut(s) 5, 194, 267, 309, 822
BspLI GGNNCC 3 cut(s) 415, 758, 986
BspMAI CTGCAG 2 cut(s) 980, 1028
BspMI ACCTGC 1 cut(s) 969
BspPI GGATC 2 cut(s) 172, 775
BspQI GCTCTTC 1 cut(s) 348
BspT107I GGYRCC 1 cut(s) 984
BspTNI GGTCTC 1 cut(s) 1043
BsrDI GCAATG 2 cut(s) 1069, 1099
BsrFI RCCGGY 2 cut(s) 267, 987
BsrGI TGTACA 1 cut(s) 639
BsrI ACTGG 4 cut(s) 635, 783, 807, 884
BssAI RCCGGY 2 cut(s) 267, 987
BssECI CCNNGG 2 cut(s) 700, 1053
BssMI GATC 6 cut(s) 33, 177, 429, 767, 835, 1135
BssNI GRCGYC 1 cut(s) 382
BssSI CACGAG 1 cut(s) 237
BssT1I CCWWGG 1 cut(s) 1053
Bst2BI CACGAG 1 cut(s) 237
Bst4CI ACNGT 1 cut(s) 128
Bst6I CTCTTC 3 cut(s) 93, 176, 348
BstACI GRCGYC 1 cut(s) 382
BstAPI GCANNNNNTGC 2 cut(s) 572, 984
BstAUI TGTACA 1 cut(s) 639
BstC8I GCNNGC 6 cut(s) 227, 269, 408, 707, 927, 989
BstDEI CTNAG 1 cut(s) 789
BstDSI CCRYGG 1 cut(s) 1053
BstENI CCTNNNNNAGG 1 cut(s) 1011
BstF5I GGATG 4 cut(s) 417, 867, 938, 1022
BstH2I RGCGCY 1 cut(s) 620
BstHHI GCGC 2 cut(s) 251, 619
BstKTI GATC 6 cut(s) 36, 180, 432, 770, 838, 1138
BstMAI GTCTC 4 cut(s) 240, 685, 1043, 1132
BstMBI GATC 6 cut(s) 33, 177, 429, 767, 835, 1135
BstNSI RCATGY 1 cut(s) 410
BstPAI GACNNNNGTC 1 cut(s) 209
BstSFI CTRYAG 2 cut(s) 976, 1024
BstV1I GCAGC 4 cut(s) 288, 526, 626, 1010
BstX2I RGATCY 1 cut(s) 767
BstYI RGATCY 1 cut(s) 767
Bsu36I CCTNAGG 1 cut(s) 789
BsuI GTATCC 1 cut(s) 1013
BsuRI GGCC 5 cut(s) 5, 194, 267, 309, 822
BtgI CCRYGG 1 cut(s) 1053
BtsCI GGATG 4 cut(s) 417, 867, 938, 1022
BtsI GCAGTG 2 cut(s) 448, 1162
BtsIMutI CAGTG 3 cut(s) 66, 448, 1162
BveI ACCTGC 1 cut(s) 969
Cac8I GCNNGC 6 cut(s) 227, 269, 408, 707, 927, 989
CfoI GCGC 2 cut(s) 251, 619
Cfr10I RCCGGY 2 cut(s) 267, 987
Cfr13I GGNCC 3 cut(s) 308, 351, 482
CseI GACGC 1 cut(s) 371
Csp6I GTAC 3 cut(s) 528, 640, 973
CspCI CAANNNNNGTGG 2 cut(s) 220, 255
CviAII CATG 6 cut(s) 156, 378, 399, 407, 1033, 1054
CviQI GTAC 3 cut(s) 528, 640, 973
DdeI CTNAG 1 cut(s) 789
DpnI GATC 6 cut(s) 35, 179, 431, 769, 837, 1137
DpnII GATC 6 cut(s) 33, 177, 429, 767, 835, 1135
DraIII CACNNNGTG 2 cut(s) 190, 427
EaeI YGGCCR 2 cut(s) 3, 265
Eam1104I CTCTTC 3 cut(s) 93, 176, 348
EarI CTCTTC 3 cut(s) 93, 176, 348
Ecl136II GAGCTC 2 cut(s) 341, 584
Eco130I CCWWGG 1 cut(s) 1053
Eco147I AGGCCT 1 cut(s) 822
Eco24I GRGCYC 2 cut(s) 343, 586
Eco31I GGTCTC 1 cut(s) 1043
Eco47I GGWCC 2 cut(s) 351, 482
Eco47III AGCGCT 1 cut(s) 618
Eco53kI GAGCTC 2 cut(s) 341, 584
Eco57I CTGAAG 3 cut(s) 8, 890, 1087
Eco81I CCTNAGG 1 cut(s) 789
EcoICRI GAGCTC 2 cut(s) 341, 584
EcoNI CCTNNNNNAGG 1 cut(s) 1011
EcoRI GAATTC 1 cut(s) 459
EcoT14I CCWWGG 1 cut(s) 1053
EcoT38I GRGCYC 2 cut(s) 343, 586
ErhI CCWWGG 1 cut(s) 1053
FaeI CATG 6 cut(s) 159, 381, 402, 410, 1036, 1057
FalI AAGNNNNNCTT 2 cut(s) 92, 124
FaqI GGGAC 1 cut(s) 1043
FatI CATG 6 cut(s) 155, 377, 398, 406, 1032, 1053
FauI CCCGC 2 cut(s) 128, 306
FbaI TGATCA 1 cut(s) 429
Fnu4HI GCNGC 6 cut(s) 6, 139, 302, 540, 615, 1024
FokI GGATG 4 cut(s) 404, 874, 945, 1029
FriOI GRGCYC 2 cut(s) 343, 586
Fsp4HI GCNGC 6 cut(s) 6, 139, 302, 540, 615, 1024
FspBI CTAG 1 cut(s) 662
GlaI GCGC 2 cut(s) 250, 618
GluI GCNGC 6 cut(s) 6, 139, 302, 540, 615, 1024
GsaI CCCAGC 1 cut(s) 566
GsuI CTGGAG 1 cut(s) 600
HaeII RGCGCY 1 cut(s) 620
HaeIII GGCC 5 cut(s) 5, 194, 267, 309, 822
HapII CCGG 3 cut(s) 268, 411, 988
HgaI GACGC 1 cut(s) 371
HhaI GCGC 2 cut(s) 251, 619
Hin1I GRCGYC 1 cut(s) 382
Hin1II CATG 6 cut(s) 159, 381, 402, 410, 1036, 1057
Hin6I GCGC 2 cut(s) 249, 617
HinP1I GCGC 2 cut(s) 249, 617
HincII GTYRAC 1 cut(s) 217
HindII GTYRAC 1 cut(s) 217
HindIII AAGCTT 1 cut(s) 20
HinfI GANTC 4 cut(s) 71, 367, 622, 967
HpaII CCGG 3 cut(s) 268, 411, 988
HphI GGTGA 2 cut(s) 1069, 1153
Hpy166II GTNNAC 4 cut(s) 217, 642, 654, 721
Hpy188I TCNGA 4 cut(s) 27, 292, 627, 797
Hpy188III TCNNGA 6 cut(s) 80, 92, 175, 579, 809, 1133
Hpy8I GTNNAC 4 cut(s) 217, 642, 654, 721
Hpy99I CGWCG 3 cut(s) 132, 147, 351
HpyAV CCTTC 3 cut(s) 118, 205, 321
HpyCH4III ACNGT 1 cut(s) 128
HpyCH4IV ACGT 1 cut(s) 530
HpyCH4V TGCA 9 cut(s) 406, 441, 542, 614, 944, 952, 978, 1026, 1155
HpyF3I CTNAG 1 cut(s) 789
HpySE526I ACGT 1 cut(s) 530
Hsp92I GRCGYC 1 cut(s) 382
Hsp92II CATG 6 cut(s) 159, 381, 402, 410, 1036, 1057
HspAI GCGC 2 cut(s) 249, 617
KroI GCCGGC 2 cut(s) 267, 987
KroNI GCCGGC 2 cut(s) 269, 989
Ksp22I TGATCA 1 cut(s) 429
Kzo9I GATC 6 cut(s) 33, 177, 429, 767, 835, 1135
LguI GCTCTTC 1 cut(s) 348
LmnI GCTCC 7 cut(s) 82, 338, 413, 469, 581, 602, 1020
Lsp1109I GCAGC 4 cut(s) 288, 526, 626, 1010
MaeI CTAG 1 cut(s) 662
MaeII ACGT 1 cut(s) 530
MaeIII GTNAC 1 cut(s) 594
MalI GATC 6 cut(s) 35, 179, 431, 769, 837, 1137
MboI GATC 6 cut(s) 33, 177, 429, 767, 835, 1135
MboII GAAGA 5 cut(s) 80, 103, 163, 335, 1103
MflI RGATCY 1 cut(s) 767
MhlI GDGCHC 2 cut(s) 343, 586
MluCI AATT 2 cut(s) 459, 691
MmeI TCCRAC 1 cut(s) 436
MroNI GCCGGC 2 cut(s) 267, 987
MroXI GAANNNNTTC 1 cut(s) 87
MseI TTAA 2 cut(s) 488, 500
MslI CAYNNNNRTG 1 cut(s) 397
MspA1I CMGCKG 2 cut(s) 301, 1072
MspI CCGG 3 cut(s) 268, 411, 988
NaeI GCCGGC 2 cut(s) 269, 989
NcoI CCATGG 1 cut(s) 1053
NdeII GATC 6 cut(s) 33, 177, 429, 767, 835, 1135
NgoMIV GCCGGC 2 cut(s) 267, 987
NlaIII CATG 6 cut(s) 159, 381, 402, 410, 1036, 1057
NlaIV GGNNCC 3 cut(s) 415, 758, 986
NmeAIII GCCGAG 1 cut(s) 681
NspI RCATGY 1 cut(s) 410
PaeI GCATGC 1 cut(s) 410
PaqCI CACCTGC 1 cut(s) 969
PceI AGGCCT 1 cut(s) 822
PciSI GCTCTTC 1 cut(s) 348
PcsI WCGNNNNNNNCGW 1 cut(s) 142
PdiI GCCGGC 2 cut(s) 269, 989
PdmI GAANNNNTTC 1 cut(s) 87
PfeI GAWTC 4 cut(s) 71, 367, 622, 967
PflMI CCANNNNNTGG 1 cut(s) 569
PkrI GCNGC 6 cut(s) 7, 140, 303, 541, 616, 1025
PshAI GACNNNNGTC 1 cut(s) 209
PshBI ATTAAT 1 cut(s) 488
Psp124BI GAGCTC 2 cut(s) 343, 586
PspFI CCCAGC 1 cut(s) 562
PspN4I GGNNCC 3 cut(s) 415, 758, 986
PspPI GGNCC 3 cut(s) 308, 351, 482
PstI CTGCAG 2 cut(s) 980, 1028
PsuI RGATCY 1 cut(s) 767
PvuII CAGCTG 1 cut(s) 1072
RsaI GTAC 3 cut(s) 529, 641, 974
RsaNI GTAC 3 cut(s) 528, 640, 973
RseI CAYNNNNRTG 1 cut(s) 397
SacI GAGCTC 2 cut(s) 343, 586
SapI GCTCTTC 1 cut(s) 348
SaqAI TTAA 2 cut(s) 488, 500
SatI GCNGC 6 cut(s) 6, 139, 302, 540, 615, 1024
Sau3AI GATC 6 cut(s) 33, 177, 429, 767, 835, 1135
Sau96I GGNCC 3 cut(s) 308, 351, 482
ScaI AGTACT 1 cut(s) 974
SduI GDGCHC 2 cut(s) 343, 586
SfcI CTRYAG 2 cut(s) 976, 1024
SinI GGWCC 2 cut(s) 351, 482
SmiMI CAYNNNNRTG 1 cut(s) 397
SmlI CTYRAG 2 cut(s) 807, 1131
SmoI CTYRAG 2 cut(s) 807, 1131
SphI GCATGC 1 cut(s) 410
Sse9I AATT 2 cut(s) 459, 691
SseBI AGGCCT 1 cut(s) 822
SsiI CCGC 6 cut(s) 6, 53, 121, 139, 299, 315
SspMI CTAG 1 cut(s) 662
SstI GAGCTC 2 cut(s) 343, 586
StuI AGGCCT 1 cut(s) 822
StyI CCWWGG 1 cut(s) 1053
TaaI ACNGT 1 cut(s) 128
TaiI ACGT 1 cut(s) 533
TaqI TCGA 4 cut(s) 36, 145, 346, 731
TasI AATT 2 cut(s) 459, 691
TatI WGTACW 2 cut(s) 639, 972
TauI GCSGC 2 cut(s) 8, 141
TfiI GAWTC 4 cut(s) 71, 367, 622, 967
Tru1I TTAA 2 cut(s) 488, 500
Tru9I TTAA 2 cut(s) 488, 500
TscAI CASTG 3 cut(s) 73, 448, 1162
TseI GCWGC 4 cut(s) 301, 539, 614, 1023
TspDTI ATGAA 2 cut(s) 345, 1080
TspGWI ACGGA 1 cut(s) 364
TspRI CASTG 3 cut(s) 73, 448, 1162
Van91I CCANNNNNTGG 1 cut(s) 569
VpaK11BI GGWCC 2 cut(s) 351, 482
VspI ATTAAT 1 cut(s) 488
XagI CCTNNNNNAGG 1 cut(s) 1011
XapI RAATTY 2 cut(s) 459, 691
XceI RCATGY 1 cut(s) 410
XmnI GAANNNNTTC 1 cut(s) 87
XspI CTAG 1 cut(s) 662
ZrmI AGTACT 1 cut(s) 974
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.